Claims
- 1. In antibiotic and steroid transformation processes, wherein an immobilized living microorganism is used as a catalyst to transform a substrate which is an antibiotic or a steroid, the method of activating said catalyst comprising adding to a reaction mixture containing said catalyst and substrate to be transformed thereby, peptone or a mixture of peptone and glucose.
- 2. The method according to claim 1 wherein said substrate is cortisol or a cortisol derivative selected from the group consisting of 6 alpha-, 9 alpha-difluoro derivative, 6 alpha fluoro-, 16 alpha-methyl derivative, 9 alpha fluoro-, 16 alpha-hydroxy derivative, and 6 alpha, 9 alpha difluoro 16 alpha-, 17 alpha-isopropylidenedioxy derivative, and is delta 1-dehydrogenated to the corresponding prednisolone compound in said reaction mixture.
- 3. The method according to claim 1 wherein said substrate is a steroid which is transformed by a 11- or 16-hydroxylation thereof.
- 4. The method according to claim 1 wherein said catalyst is a microorganism transforming the steroid by dehydrogenation transformation thereof.
- 5. The method according to claim 1 wherein a steroid transformation is catalyzed by Arthrobacter simplex.
- 6. The method according to claim 1 wherein the catalyst is a microorganism entrapped in polyacrylamide gel.
- 7. The method according to claim 1, wherein the amount of peptone or a mixture of peptone and glucose added to the reaction mixture is between 0.1-1.0% (weight/volume).
Parent Case Info
This is a continuation of application Ser. No. 812,996 filed July 5, 1977 and now abandoned.
US Referenced Citations (5)
Non-Patent Literature Citations (3)
Entry |
Biotechnology and Bioengineering vol. XIII, pp. 503-515, (1971). |
J. Gen. Appl. Microbiol. vol. 7, No. 2, 1961, pp. 113-117. |
Applied Microbiology vol. 27, pp. 878-885, (1974). |
Continuations (1)
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Number |
Date |
Country |
Parent |
812996 |
Jul 1977 |
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