Claims
- 1. A process for production of antibiotic BMG162-aF2, which comprises cultivating a BMG162-aF2-producing strain of Bacillus laterosporus having the identifying characteristics of ATCC 31932 or a BMG162-aF2-producing mutant thereof in an aqueous nutrient medium to produce and accumulate BMG162-aF2 and then recovering from the culture medium the antibiotic BMG162-aF2 having the formula ##STR10##
- 2. The process according to claim 1 which includes the additional step of reacting the antibiotic BMG162-aF2 with a pharmaceutically acceptable acid to form a pharmaceutically acceptable acid addition salt of BMG162-aF2.
- 3. The process according to claim 1 or 2 wherein the culture medium is one containing nitrogen sources, carbon sources, and inorganic salts.
- 4. The process according to claim 1 wherein the cultivation is carried out aerobically at 15.degree. to 40.degree. C., at pH 5.0 to 8.2.
- 5. The process according to claim 1 wherein the recovery of the BMG162-aF2 from the culture medium is carried out by means of column chromatography using a weak cationic ion exchange resin having carboxylic acid as an active group.
- 6. The process according to claim 5, wherein the BMG162-aF2 adsorbed on the resin is eluted with an aqueous acid solution or an aqueous salt solution.
- 7. A biologically pure culture of the microorganism Bacillus laterosporus BMG162-aF2, ATCC 31932, said culture being capable of producing the antibiotic BMG162-aF2 of the formula ##STR11## in a recoverable quantity upon cultivation in an aqueous nutrient medium.
Priority Claims (1)
Number |
Date |
Country |
Kind |
55-123585 |
Sep 1980 |
JPX |
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Parent Case Info
This is a division of application Ser. No. 297,458, filed Aug. 28, 1981 now U.S. Pat. No. 4,416,899.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
2998438 |
Charney et al. |
Aug 1961 |
|
3617448 |
Mann |
Nov 1971 |
|
3743580 |
Umezawa et al. |
Jul 1973 |
|
Non-Patent Literature Citations (2)
Entry |
Shoji et al., J. Antibiotics, vol. 29, No. 4, 1976, pp. 390-393. |
Kido et al., J. Antibiotics, vol. 33, No. 8, 1980, pp. 791-795. |
Divisions (1)
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Number |
Date |
Country |
Parent |
297458 |
Aug 1981 |
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