Antibiotic C-11924 F-1

This invention relates to a novel antibiotic C-11924 F-1 and a method for the production thereof.
In search of novel antibiotics, we isolated microorganisms from a large number of soil samples and separated and examined the antibiotics elaborated by such microorganisms. As a result, we found that certain of those microorganisms elaborate a novel antibiotic, that those microorganisms belong to the genus Streptoverticillium and that, when cultivated in an appropriate medium, such microorganisms accumulate said antibiotic in the cultured broth. We designated this antibiotic "Antibiotic C-11924 F-1".
Antibiotic C-11924 F-1 (hereinafter referred to briefly as C-11924 F-1) producing strain, as aforesaid, belongs to the genus Streptoverticillium. Streptoverticillium cinnamoneum No. C-11924 (hereinafter referred to briefly as Strain No. C-11924) which we isolated from a soil sample obtained at Gero, Gifu Prefecture, Japan is one of the microorganisms which can be most advantageously utilized in the method of this invention. The above strain of microorganism has been deposited at the Institute for Fermentation, Osaka under the accession number of IFO 13713, at the Fermentation Research Institute of the Agency of Industrial Technology under receipt number 3837 of written request for the deposition, and at the American Type Culture Collection, U.S.A. under the accession number of ATCC-31364. The microbiological characteristics of Strain No. C-11924 as investigated by Sherling & Gottlieb's method (International Journal of Systematic Bacteriology, Vol. 16, p. 313-340, 1966) for 3 weeks are as follows.
(1) Morphological characters
Sporulated hyphae in whorls. Cylindrical or phalanx-shaped spores (0.5-0.7.times.0.9-1.1.mu.) are produced; smooth surfaces.
(2) Cultural characteristics
This strain produces a cottony or velvety aerial mycelium, whitish-yellow, pinkish gray to pinkish purple gray. The substrate mycelium is yellow to yellowish tan, and substantially no soluble pigments are produced. No production of melanoid pigments.
The cultural characters, physiological characters and carbon assimilation spectrum of this strain are shown in Tables 1 to 3, respectively.
Table 1__________________________________________________________________________Cultural characteristics on various media Reverse color of Surface color of Growth on surface ofMedium substrate mycelium substrate mycelium colony, and its color Soluble pigment__________________________________________________________________________Sucrose nitrate agar Colorless Colorless Sparse, light pink None (4 ca)Glucose asparagine Light yellow(2 ea)- Light yellow (2 ea)- Abundant, white with Noneagar dull yellow orange dull yellow orange a tinge of bright (3 ic) (3 ic) brownish gray(3 ec)Glycerol-asparagine Light yellow(2 ga)- Light yellow(2 ga)- Moderate, limited Noneagar dull yellow(2 ic) dull yellow(2 ic) growth, beige with a tinge of pink(4 ec)Inorganic salts- Light yellow(2 ga) Light yellow(2 ga) Velvety abundant Nonestarch agar growth, beige with a tinge of pink(4 ec)Tyrosine agar Light yellow(2 ca)- Light yellow(2 ca)- Moderate growth, None yellowish tan(3 ne) yellowish tan(3 ne) creased and limited; whitish to yellowNutrient agar Brown(4 ni)-dark tan Brown (4 ni) (Moderate, bright None brownish gray(3 ec)Yeast extract-malt Yellowish orange Yellowish orange Velvety, good growth, Noneextract agar (3 nc) to yellowish (3 nc) to yellowish bright brownish brown (3 ne) brown (3 ne) gray (3 ec)Oatmeal-agar Dull yellow(2 lc) Dull yellow(2 lc) Velvety, good growth, None or very bright brownish gray light yellowish (3 ec)(3 cb) brown__________________________________________________________________________ The parenthesized symbols indicate the color codes according to Color Harmony Manual, 4th ed. (Container Corporation of America, 1958).
Table 2______________________________________Physiological characteristics______________________________________Temperature range for growth 10.degree.-40.degree. C.Liquefaction of gelatin PositiveHydrolysis of starch PositiveCoagulation of skimmed milk NegativePeptonization of skimmed milk PositiveProduction of melanoid pigments I) Tyrosine agar Negative II) Peptone-yeast extract iron agar NegativeReduction of nitrates Negative______________________________________
Table 3______________________________________Assimilation of carbon sources Growth______________________________________L-arabinose .+-.D-xylose .+-.D-glucose +++D-fructose + to ++D-galactose +D-trehalose +++Sucrose .+-.L-rhamnose .+-.Raffinose .+-.i-Inositol +++D-mannitol .+-.Control (not added) .+-.______________________________________ +++: Luxuriant growth +: Growth ++: Good growth .+-.: Poor growth -: No growth
Comparison of the above-described characteristics with the corresponding descriptions in S.A. Waksman's The Actinomycetes, Vol. 2, 1961, The Williams and Wilkins Company; E.B. Scherling and D. Gottlieb's reports in International Journal of Systematic Bacteriology 18, 69 (1968), do. 18, 279 (1968), do. 19, 391 (1969) and do. 22, 265 (1972); R. Locci et al's report in Giornale di Microbiologia 17, 1-60 (1969); and Bergey's Manual of Determinative Bacteriology, 8 ed., (1974), The Williams and Wilkins Company shows that Strain No. C-11924 is similar to Streptoverticillium cinnamoneum and Streptoverticillium hachijoense. And, for comparison's sake, we cultivated on various media the present strain along with the known strains available from the Institute for Fermentation, Osaka, namely Streptoverticillium cinnamoneum IFO-12852 (ISP 5005), Streptomyces cinnamomeus f. azacoluta IFO-12363 (Streptoverticillium cinnamoneum f. azacoluta ) and Streptomyces hachijoensis, IFO-12782 (ISP 5114) (Streptoverticillium hachijoense). As will be seen from Table 4, Strain No. C-11924 bears a close resemblance with these three strains of microorganisms in the color of aerial mycelium, physiological characters, carbon utilization spectrum, etc. However, Streptomyces hachijoensis differentiates itself from Strain No. C-11924 in that the former cannot grow on glycerol-asparagine agar or tyrosine agar medium. Strain No. C-11924 appears to resemble Streptomyces cinnamomeus f. azacoluta more closely in that they have a light yellow to dull yellow substrate mycelium and peptonize milk but this latter strain differentiates itself from Strain No. C-11924 in that it cannot grow on tyrosine agar. Furthermore, Strain No. C-11924 differs from those known organisms in that it gives a light olive aerial mycelium on glycerol nitrate agar. Based on the above findings, we have identified Strain No. C-11924 as a strain of Streptoverticillium cinnamoneum and designated it Streptoverticillium cinnamoneum No. C-11924.
Table 4__________________________________________________________________________Comparison of Strain No. C-11924 with known species Streptomyces Strain No. cinnamomeus f. Streptomyces StreptoverticilliumMedium C-11924 azacoluta hachijoensis cinnamoneum__________________________________________________________________________Sucrose Growth Poor, colorless Poor, colorless Moderate Poor, colorlessnitrateagar Aerial Sparse, light pink Sparse, light pink Moderate, light beige Sparse, light pink mycelium Reverse Colorless Colorless Light beige Colorless Soluble pigment None None None NoneGlycerol Growth Good, creased Good Good, creased Good, limited growthnitrateagar Aerial Moderate, light Abundant, velvety, Moderate, white with Moderate, bright mycelium olive white with a tinge a tinge of beige brownish gray of pink Reverse Light yellow Light yellow Yellowish brown Brown to dark tan Soluble None, or very None or very light pigment light yellow yellow None NoneGlycerol Growth Moderate, limited Good, creased and No growth Moderate, limitedasparagine growth raised growthagar Aerial Abundant, beige Abundant, beige Moderate, beige with(ISP-5) mycelium with a tinge of with a tinge of a tinge of pink pink pink Reverse Light yellow to Dull yellow Dark brown dull yellow Soluble pigment None None NoneInorganic Growth Good Good Good Moderate to goodsalts-starch Aerial Abundant, velvety; Abundant, velvety; Abundant, velvety, Abundant, beige withagar mycelium beige with a tinge beige with a tinge beige with a tinge a tinge of pink(ISP-4) of pink of pink of pink Reverse Light yellow Light yellow Light yellow Brown to dark tan Soluble pigment None None None NoneTyrosine Growth Moderate, limitedagar and creased growth No growth No growth No growth(ISP-7) Aerial Sparse, whitish to mycelium yellow Reverse Yellowish brown Soluble pigment NoneLiquefactionof gelatin +++ +++ +++ +Peptonizationof milk +++ +++ +++ -Hydrolysisof starch +++ +++ +++ +++Reduction ofnitrates - - - -Utilization ofcarbohydratesMannitol .+-. .+-. .+-. -Inositol +++ +++ +++ +Glycerol +++ +++ +++ ++ to +++Glucose +++ +++ +++ -Arabinose .+-. .+-. .+-. -Xylose .+-. .+-. .+-. -Rhamnose .+-. .+-. .+-. -Fructose + to ++ ++ + to ++ +Raffinose .+-. .+-. .+-. .+-.Mannose +++ +++ +++ ++Starch +++ +++ +++ +++Control .+-. .+-. .+-. -__________________________________________________________________________ +++: Luxuriant growth ++: Good growth +: Growth .+-.: Poor growth -: No growth
On the other hand, in view of the fact that the novel antibiotic C-11924 produced by Strain No. C-11924 belongs to the phleomycin-bleomycin group of antibiotics, Strain No. C-11924 was compared with the microorganisms known to produce antibiotics of this group.
Thus, Strain No. C-11924 differentiates from Streptomyces verticillus, the phleomycin-bleomycin-producing strain described in Journal of Antibiotics 12 A, 111 (1959), 19 A, 200-209 (1966), in that the latter has an aerial mycelium white to gray or gray with a tinge of green. Strain No. C-11924 differs also clearly from the following strains in sporulated hyphae and other morphological features. Streptomyces flavoviridis (Japanese Patent Application Laidopen No. 22687/1973); Streptomyces bikiniensis var. zorbonensis, the zorbamycin-producing strain described in Journal of Bacteriology 105, 880-885 (1971); Streptomyces humidus var. antitumoris, YA-56X and Y-producer as described in Journal of Antibiotics 26, 70-76 (1973); Streptosporangium violaceochromogenes, an XK-49-1B-2-producer as described in Japanese Patent Application Laid-open No. 42896/1974; and Streptomyces olivogriseus, the SS-70A and B-producing microorganism described in Japanese Patent Application Laidopen No. 15693/1976.
Like other microorganisms of the genus Streptoverticillium, Strain No. C-11924 is liable to change its characters, being ready to undergo mutation when subjected to an artificial mutagenic treatment such as irradiation with ultraviolet light, X-rays or other radiation or with an artificial mutagen. It should be understood than even such mutants can invariably be utilized in the method of this invention only if they are capable of producing C-11924 F-1.
In the method of this invention, the medium for cultivation of Strain No. C-11924 may be whichever of a fluid medium and a solid medium, although fluid media are more convenient. Either surface culture or shaking culture may be carried out, submerged culture being more advantageous. In the medium are incorporated carbon sources which Strain No. C-11924 is able to assimilate, such as starch, glucose, dextrin, glycerin, sucrose, n-paraffin, alcohols (e.g. methanol), etc. and nitrogen sources such as corn steep liquor, soybean flour, cottonseed flour, peptone, meat extract, urea, etc. by way of organic nitrogen sources, or ammonium chloride, ammonium sulfate, ammonium nitrate, etc. by way of inorganic nitrogen sources. If necessary, there may be incorporated appropriate amounts of inorganic salts such as salts including sodium, potassium, magnesium, calcium or phosphorus salts heavy metal salts such as salts of iron, manganese, zinc, cobalt, copper, nickel, etc.; antifoams such as soybean oil, lard oil, chicken oil, silicone oil, Actocol (Takeda Chemical Industries Ltd., Japan), etc. In carrying out a fluid culture, the pH of the medium is preferably maintained near neutral and, particularly, at pH 6 to 8. The incubation temperature and time are preferably 24.degree. C. to 30.degree. C. and 90 to 140 hours, respectively. The change in the titer of activity with the time of cultivation can be monitored by the paper disc method using Salmonella typhimurium IFO 12529 as the assay organism (assay medium: Trypticase soy agar, BBL).
C-11924 F-1 as obtainable by this invention is a copper-containing basic peptide antibiotic and can be separated, recovered and purified from the cultured broth by procedures commonly employed. Thus, concentration under reduced pressure, lyophilization, solvent extraction, dialysis, adsorption on activated carbon, silica gel, alumina or the like, or on a synthetic adsorbent of the macroporous nonionic type, e.g. Amberlite XAD-2, Diaion HP-10 or the like, weakly acidic ion exchange resin, gel-filtration agent, ion exchange-gel filtration agent, etc. may be employed in a suitable and optional combination or in repetition.
An exemplary purification procedure may be as follows.
Because C-11924 F-1 occurs in the liquid phase of the cultured broth, the cells are first removed from the cultured broth by means of a filtration aid such as diatomaceous earth and the active component of the filtrate is adsorbed on a column of Diaion HP-10 (Mitsubishi Kasei K.K. in Japan). After a thorough aqueous rinse, the active component is eluted with a mixture of water and one or more hydrophilic solvents such as lower ketones, e.g. acetone, methyl ethyl ketone, or lower alcohols, e.g. methanol, ethanol, isopropanol, n-propanol, n-butanol, etc. The active fractions are concentrated under reduced pressure to remove the organic solvent and the concentrate is subjected to molecular-sieve chromatography on a gel filtration agent. First, using a column of Sephadex LH-20, (Pharmacia Fine Chemicals AB, Sweden), solvent-soluble impurities are removed by development with aqueous alcohol and the active fractions are concentrated under reduced pressure and lyophilized to obtain crude powders. To purify the crude powders, column chromatography with an ion exchange-gel filtration agent is most effective but, because of the basic nature of the substance, CM-Sephadex C-25 will prove most effective. The crude powders are dissolved in a dilute solution of formic acid in ammonia and the solution is adsorbed on CM-Sephadex C-25, elution being carried out with formic acid-ammonia by the gradient method. The active fractions emerging as a single peak at 0.32 to 0.37 mol concentration of formic acid-ammonia are collected and adsorbed on a column of Diaion HP-10. Elution is carried out with aqueous alcohol and the desalted eluate is lyophilized. By this procedure is obtained C-11924F-1 which is paper chromatographically a single substance.
The physical and chemical properties of C-11924F-1 obtained in Example 1 are as follows.
1. Appearance: Blue amorphous powder
______________________________________2. Elemental analysis:______________________________________ C: 40.81; 40.87; 40.55 H: 5.72; 5.02; 5.55 N: 16.35; 16.58; 16.40 S: 3.95; 3.97; 3.50 Cu: 3.64; 3.46; 3.29______________________________________
3. Melting point (decomp.): not lower than 195.degree. C. (no definite decomposition point)
4. Molecular weight:
(I) Vapor-pressure osmosis: n.multidot.(1.0.times.10.sup.3)(n is an integer) (distilled water) It is quite within the bounds of possibility that n is 2.
(II) Molecular weight as calculated on the assumption that each molecule contains one atom of copper: 1800.+-.50
5. Ultraviolet absorption spectrum:
As shown in FIG. 1, there are absorption maxima in aqueous solution at 243 nm.+-.2(E.sub.1 cm.sup.1% 167.+-.16) and 297 nm.+-.2 (E.sub.1 cm.sup.1% 55.+-.6).
6. Infrared absorption spectrum:
The spectrum obtained by the KBr method is shown in FIG. 2. Principal peaks in the infrared absorption spectrum are as follows (cm.sup.-1).
3350, 2920, 1715, 1650, 1625, 1600, 1550, 1520, 1450, 1370, 1345, 1090, 1060, 1005, 980
7. Solubility:
Readily soluble in water; soluble in methanol; sparingly soluble in ethanol; insoluble in ethyl acetate, butyl acetate, chloroform, benzene, cyclohexane, ethyl ether and petroleum ether.
8. Color reactions:
Sakaguchi, ninhydrin, Ehrlich, Dragendorff, potassium permanganate and Greig-Leaback reactions are positive.
9. Stability:
Stable under neutral conditions; slightly unstable under alkaline conditions; unstable under acidic conditions
10. Acidity, neturality or basicity: Basic
______________________________________ 11. Paper chromatography (ascending method)Rf:Solvent system C-11924 F-1______________________________________(1) 50% Acetone water 0.20(2) 75% Phenol 0.95(3) n-Butanol-acetic acid-water (2:1:1) 0.37(4) n-Butanol-pyridine-acetic acid-water (15:10:3:12) 0.18(5) n-Butanol-acetic acid-water (upper layer of 4:1:5 mixture) 0.13(6) 3% Ammonium chloride 0.82______________________________________
For reference's sake, the corresponding paper chromatographic Rf values of YA-56-X, YA-56-Y, SS-70-A and SS-70-B are given below.
______________________________________Solvent YA-56-X YA-56-Y SS-70-A SS-70-Bsystem (Note 1) (Note 1) (Note 2) (Note 2)______________________________________(1) 0.05 0.05 0.90 0.90(2) 0.93 0.93 0.06 0.06(4) 0.30 0.45(5) 0.23 0.31(6) 0.87 0.91______________________________________ Note 1: According to J. Antibiotics 26, 77(1973). Note 2: According to Japanese Pat. Appl. Laidopen Nos. 15693/76 and 115694/76.
The present antibiotic is compared with the known antibiotics as follows.
Among the known water-soluble copper-containing basic peptide antibiotics are antibiotics of the phleomycin group (Ikekawa et al, Journal of Antibiotics 17 A, 194(1964)), antibiotics of the bleomycin group (Umezawa et al, Journal of Antibiotics 19 A, 200(1966), Zorbamycin, Zorbmycin B, Zorbonomycin C(A.D. Algoudelis et al. Journal of Antibiotics 24, 543(1971); YA-56-X and YA-56-Y(Itoh et al, Journal of Antibiotics 24, 727(1971)); XK-49-1-B-2(Jap. Pat. Appl. Laid-open No. 42896/1974); and SS-70-A and SS-70-B(Jap. Pat. Appl. Laid-open No. 15693/1976).
In view of the fact that whereas the absorbance ratio of C-11924F-1 in its ultraviolet absorption spectrum at 243 nm versus 297 nm is 3.04, the corresponding ratios for bleomycins, phleomycin C, D.sub.2 and F, zorbonomycin B and XK-49-1B-2 are in the range of 1.1 to 1.3, C-11924-F-1 is clearly distinguishable from those known antibiotics.
Based on paper chromatographic data as well, C-11924F-1 is found to be different from the known antibiotics.
However, because the ratios for phleomycin D.sub.1, E, G, H and I, zorbamycin, YA-56-X, YA-56-Y, SS-70-A and SS-70-B are in the range of 2.7 to 2.9, there is a similarity in this respect. (Incidentally, it is reported in Journal of Antibiotics 26, 77(1973) that YA-56-X and zorbamycin are the same substance). FIG. 3 shows the elution pattern of a mixture of SS-70-B and C-11924F-1 from a chromatographic column of CM-Sephadex C-25. The two components give independent peaks, indicating clearly that they are different substances. As to differences from phleomycin antibiotics, YA-56-X and Y or SS-70-A, while those substances are all eluted in advance of SS-70-B according to the description in Japanese Patent Application Laid-open No. 15694/1976, C-11924 F-1 ermerges after SS-70-B as shown in FIG. 3, thus indicating that the present antibiotic is also distinguishable from those known antibiotics.
It is apparent from the above results that C-11924 F-1 is a novel antibiotic dissimilar to any of the known antibiotics of the bleomycin-phleomycin group.
ANTIMICROBIAL SPECTRUM
The antimicrobial spectrum of C-11924 F-1 as determined by the agar dilution method using Trypticase soy agar as the assay medium (Baltimore Biologicals Limited, U.S.A.) is given in Table 5.
Table 5______________________________________Antimicrobial Spectrum Minimal inhibitory concentration,Assay organism mcg/ml C-11924F-1______________________________________Staphylococcus aureus FDA 209 P 2.0Bacillus subtilis PCI 219 1.0Bacillus cereus IFO 3514 20.0Bacillus pumilus IFO 3813 >100Escherichia coli NIHJ 0.2Proteus vulgaris IFO 3045 >100Proteus mirabilis IFO 3849 >100Pseudomonas aeruginosa IFO 3080 >100Salmonella typhimurium IFO 12529 0.1Alcaligenes faecalis IFO 13111 >100Mycobacterium sp. 607 0.5Aspergillus niger IFO 4066 5.0Candida albicans IFO 0583 >100______________________________________
TOXICITY
The acute toxicity LD.sub.50 in mice as intravenously dosed is about 200 mg/kg.
As shown by the antimicrobial spectrum given above, C-11924 F-1 according to this invention is highly active against gram-negative bacteria, gram-positive bacteria, mycobacteria and fungi and is a useful substance displaying potent germicidal activity. For instance, the present antibiotic is also of value as germicides and disinfectants against pathological microorganisms similar to the above-mentioned assay organisms.
Since the present antibiotic has therapeutic effects in mice infected with Escherichia coli, it is also an effective agent for use in the management of mammalian cases (e.g. mouse, rat, man) infected with the abovementioned bacteria.
To employ C-11924F-1 as a germicide-disinfectant, it can be used, for example, as a liquid preparation containing 5 to 200 .mu.g per ml, the concentrations depending upon the intended application. C-11924 F-1 may also be used as an ointment, for example by admixing 50 mg of it thoroughly with 10 g of petrolatum album.
C-11924 F-1 is subcutaneously administered in a form of injection liquid which is prepared by dissolving C-11924 F-1 in water or physiological saline. The daily dosage as C-11924 F-1 is 5 to 30 mg/kg.
When administered to mice inoculated with Sarcoma 180 ascites-tumor cells, C-11924 F-1 strongly inhibits the multiplication of tumor cells and has potent life-span extending effects. Therefore, C-11924 F-1 is also useful as an antitumor agent for the treatment of Sarcoma 180 ascites tumor cells in mice.

The following examples are intended to describe this invention in further detail.
In the examples "part(s)" is based on weight unless otherwise noted and the relationship between "part(s)" and "part(s) by volume" corresponds to that between "gram(s)" and "milliliter(s)", and "%" is based on "weight/volume" unless otherwise noted.
EXAMPLE 1
Fermentation tanks with 2000 part by volume capacity were each filled with 500 parts by volume of a medium made up of 3% of soluble starch, 2% of glucose, 1% of raw soybean meal, 1% of corn steep liquor, 0.5% of peptone, 0.3% of sodium chloride and 0.5% of calcium carbonate (pH 7.0). The tanks were sterilized and, then, inoculated with Streptoverticillium cinnamoneum No. C-11924(IFO 13713; FERM receipt number 3837; ATCC-31364), followed by cultivation on a reciprocating shaker at 28.degree. C. for 40 hours. One thousand five hundred parts by volume of the resultant seed culture was transferred to a tank with 200,000 part by volume capacity tank containing 100,000 parts by volume of a medium composed of 3% of glucose, 2% of soluble starch, 2% of Proflo (Traders Oil Mill Company), 0.5% of peptone, 0.3% of sodium chloride and 0.5% of calcium carbonate (pH 7.0), followed by cultivation with stirring under aerobic conditions and at 28.degree. C. for 90 hours (100 V/V % aeration, 200 r.p.m.).
To 80,000 parts by volume of the cultured broth obtained above was added 1,500 parts of diatomaceous earth as the filtration aid and the mixture was filtered under reduced pressure to obtain 66,000 parts by volume of solution. This solution (pH 7.5) was passed onto a column packed with 7,500 parts by volume of Diaion HP-10 (Mitsubishi Kasei Kogyo K.K., in Japan), whereby the active portion was adsorbed on the resin. After the column was rinsed with 30,000 parts by volume of water, it was washed with 30,000 parts by volume of 20 V/V % methanol. Elution was carried out by the gradient method using 25,000 parts by volume of 40 V/V % methanol and 25,000 parts by volume of 90 V/V % methanol, and the eluate was collected in fractions of 5,000 parts by volume. C-11924 F-1 was eluted in fractions Nos. 3 to 10. The active fractions were pooled and concentrated underreduced pressure to remove methanol and the residue was passed through a column packed with 500 parts by volume of Diaion HP-10 for a second time. The column was rinsed with 2,000 parts by volume of water and, then, elution was carried out by the gradient method using 1,500 parts by volume of 40% methanol and 1,500 parts by volume of 90 V/V % methanol. The eluate was collected in fractions of 300 parts by volume, the activity being contained in fractions Nos. 2 to 8. The active fractions were pooled, concentrated under reduced pressure and lyophilized. By this procedure was obtained 17 parts of crude powder.
The crude powder obtained above was purified on a column of Sephadex LH-20. Thus, 10 parts of the crude powder were dissolved in 20 parts by volume of 40 V/V % methanol and, after removal of insolubles, the filtrate was put on a well-rinsed column packed with 900 parts by volume of Sephadex LH-20 which has previously been well-rinsed by 40 V/V % methanol and developed with 40 V/V % methanol. The eluate was collected in 50 parts by volume fractions, the activity being contained in fractions Nos. 9 to 11. By lyophilization, 3.1 parts of crude powder was obtained. The crude powder was dissolved in 200 parts by volume of 0.05 M. ammonium formate and adsorbed on a column packed with 130 parts by volume of CM-Sephadex C-25. The column was washed with 200 parts by volume of 0.1 M. ammonium formate and elution was carried out by the gradient method using 1,000 parts by volume of 0.1 M. and 1,000 parts by volume of 1.0 M. ammonium formate. The active substance was eluted in fractions corresponding to 0.32 M. to 0.37 M ammonium formate. The eluate was adsorbed on a column packed with 30 parts by volume of Diaion HP-10, which was thoroughly rinsed with water. Then, elution was carried out with 20 V/V % methanol and, after this desalting, the eluate was lyophilized to recover 0.046 part of a pure blue product of C-11924F-1.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is an ultraviolet absorption spectrum of C-11924 F-1 (as measured in H.sub.2 O).
FIG. 2 is an infrared absorption spectrum of C-11924 F-1 (KBr).
FIG. 3 is a chromatogram of C-11924 F-1 and SS-70-B as determined by CM-Sephadex C-25(H.sup.+) and 0.1 M sodium chloride as the developer.

Antibiotic C-11924 F-1

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