Claims
- 1. A process for producing the antibiotic complex Bu-2183 which comprises cultivating Pseudomonas sp. strain D946-B83, A.T.C.C. 31086, in an aqueous nutrient medium containing assimilable sources of nitrogen and carbon under submerged aerobic conditions until a substantial amount of Bu-2183 is produced by said organism in said culture medium.
- 2. The process of claim 1 which includes the further step of recovering the Bu-2183 complex from the culture medium.
- 3. The process of claim 1 which includes the separation of the Bu-2183 complex into the individual components Bu-2183A, Bu-2183B, Bu-2183A.sub.2 and Bu-2183D by the steps of
- (a) separating the Bu-2183 complex from the mycelium;
- (b) adsorbing the complex on a cationic ion-exchange resin;
- (c) fractionally eluting the Bu-2183 components from the adsorbent; and
- (d) recovering the fractions of the desired components Bu-2183A, B, A.sub.2 and D.
CROSS-REFERENCE TO RELATED APPLICATION
This application is a divisional of co-pending U.S. application Ser. No. 627,391 filed Oct. 30, 1975, now U.S. Pat. No. 4,012,576, issued Mar. 15, 1977, which in turn is a continuation-in-part of U.S. application Ser. No. 532,137 filed Dec. 12, 1974 and now abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
3136704 |
Charney |
Jun 1964 |
|
Divisions (1)
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Number |
Date |
Country |
Parent |
627391 |
Oct 1975 |
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Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
532137 |
Dec 1974 |
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