Claims
- 1. A process for the production of an antibiotic which comprises cultivating Pseudomonas lindbergii (ATCC-31099) in a nutrient medium, containing sources of assimilable carbon and nitrogen, at temperatures ranging from about 15.degree. C to about 40.degree. C until substantial antiotic activity is imparted to said medium.
- 2. The process of claim 1 wherein the antibiotic is isolated and recovered from the nutrient medium.
- 3. The process of claim 2 wherein the nutrient medium is clarified and the antibiotic isolated and recovered by solvent extraction.
- 4. The process of claim 1 wherein the temperature of the nutrient medium ranges between about 25.degree. C to about 35.degree. C.
- 5. The process of claim 1 wherein the nutrient medium is potato, dextrose agar.
- 6. The process of claim 1 wherein the nutrient medium is maintained under aerobic conditions.
- 7. The process of claim 1 wherein a portion of the liquid of the nutrient medium is separated to provide an antibiotic concentrate which is strongly inhibitory or lethal to various fungi.
- 8. The process of claim 1 wherein an antibiotic concentrate is separated from the nutrient medium by extraction with chloroform, and subsequent evaporation of chloroform.
- 9. The antibiotic produced by the process of claim 1.
RELATED APPLICATIONS
This is a continuation-in-part of application Ser. No. 525,520, filed Nov. 20, 1974, now abandoned, and application Ser. No. 681,703, filed Apr. 29, 1976 now abandoned.
Non-Patent Literature Citations (1)
Entry |
miller, The Pfizer Handbook of Microbial Metabolite, McGraw-Hill Book Co., Inc., N.Y., N.Y., 1961, pp. 26-28. |
Related Publications (1)
|
Number |
Date |
Country |
|
681703 |
Apr 1976 |
|
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
525520 |
Nov 1974 |
|