Antibiotic rubradirin C

Abstract

Antibiotic rubradirin C producible by the controlled fermentation of the known microorganism Streptomyces achromogenes var. rubradiris, NRRL 3061. This antibiotic and its base-addition salts are active against Streptococcus pyogenes and Bacillus cereus. Accordingly, they can be used in various environments to eradicate or control such microorganisms. SuBACKGROUND OF THE INVENTIONAntibiotic rubradirin, and a microbiological process for its preparation, are disclosed in U.S. Pat. No. 3,335,057. Rubradirin B is disclosed in U.S. application Ser. No. 787,833, filed Apr. 15, 1977. Degradation products of rubradirin and rubradirin B, and processes for their preparation are disclosed in U.S. application Ser. No. 793,785, filed May 5, 1977.BRIEF SUMMARY OF THE INVENTIONThe novel antibiotic of the invention, rubradirin C, is obtained by culturing Streptomyces achromogenes var. rubradiris, NRRL 3061, in an aqueous nutrient medium under aerobic conditions. The fermentation conditions disclosed in U.S. Pat. No. 3,335,057, referred to above, can be used to prepare rubradirin C. However, the fermentation procedures disclosed herein are preferred for the preparation of rubradirin C.

Description

Claims

1. Essentially pure rubradirin C, a compound which

(a) is effective in inhibiting the growth of Streptococcus pyogenes and Bacillus cereus;

(b) is soluble in dimethylformamide, dimethylsulfoxide and aqueous base, and is insoluble in aqueous acid and hydrocarbon solvents;

(c) has the following elemental analysis: C, 60.77; H, 4.45; N, 5.10;

(d) has a molecular weight of 779 (determined by field desorption mass spectroscopy);

(e) has a characteristic infrared absorption spectrum as shown in FIG. 1 of the accompanying drawings;

(f) has a characteristic ultraviolet absorption spectrum as shown in FIG. 2 of the accompanying drawings;

(g) has a melting point >300.degree. Dec.; and,

(h) has a molecular formula C.sub.40 H.sub.33 N.sub.3 O.sub.14.

2. Rubradirin C, as defined in claim 1, in its crystalline form.

3. A compound selected from the group consisting of rubradirin C, according to claim 1, and salts thereof with alkali metal or alkaline earth metals.

4. Sodium salt of rubradirin C, said rubradirin C defined in claim 1.