Claims
- 1. A method for assaying for cancer in a mammal comprising:
attaching a trapping antibody to a surface, wherein the trapping antibody recognizes extracellular matrix (ECM) complexes; contacting a biological fluid sample with the trapping antibody attached to the surface to thereby bind any ECM complex present in the biological fluid to the trapping antibody to form bound ECM complex; preparing signaling antibody, wherein the signaling antibody recognizes ECM complex; binding signaling antibodies to bound ECM complex; and quantitating the amount of signaling antibody bound to the surface, wherein accumulation of signaling antibody on the surface, above a background accumulation, indicates a positive result for cancer.
- 2. A method for as recited in claim 1 wherein the antibodies are selected from the group consisting of monoclonal antibodies and polyclonal antibodies.
- 3. A method for as recited in claim 1 wherein the background accumulation is the accumulation obtained with the serum from a normal population.
- 4. A method as recited in claim 1 wherein the trapping antibody is prepared by a method comprising:
isolating an impure antibody composition; contacting the impure antibody composition with ECM-complex-chromatography-medium to bind antibodies to the ECM complex; and eluting the bound antibodies from the ECM-complex-chromatography-medium.
- 5. A method as recited in claim 1 wherein the signal antibody is prepared by a method comprising:
isolating an impure antibody composition; contacting the impure antibody composition with ECM-complex-chromatography-medium to bind antibodies to the ECM complex; eluting the bound antibodies from the ECM-complex-chromatography-medium; and binding a label to the antibodies eluted from the ECM-complex-chromatography-medium.
- 6. The method as recited in claim 5 wherein the label is selected from the group consisting of enzyme labels, biotin labels, fluorescent labels and radioactive labels.
- 7. The method as recited in claim 1 wherein biological fluids which indicates a positive result for cancer are further tested to identify the type of cancera present by reacting the body fluids with anti-collagen antibodies.
- 8. A method of purifying trapping antibodies comprising:
providing an impure antibody composition; contacting the impure antibody composition ECM-complex-chromatography-medium to bind antibodies to the ECM complex; and eluting the bound antibodies from the ECM-complex-chromatography-medium.
- 9. The method as recited in claim 8 wherein the method further comprises contacting the antibodies eluted from the ECM-complex-chromatography-medium with normal-serum-chromatography-medium to bind contaminants to the normal-serum-chromatography-medium.
- 10. The method as recited in claim 8 wherein the method further comprises contacting the antibodies eluted from the ECM-complex-chromatography-medium with normal-plasma-chromatography-medium to bind contaminants to the normal-plasma-chromatography-medium.
- 11. The method as recited in claim 8 wherein the method further comprises contacting the antibodies eluted from the ECM-complex-chromatography-medium with type-IV-collagen-chromatography-medium to bind contaminants to the type-IV-collagen-chromatography-medium.
- 12. The method as recited in claim 8 wherein the method further comprises contacting the impure antibody composition with type-VI-collagen-chromatography-medium to bind antibodies to the type VI collagen.
- 13. A method as recited in claim 8 wherein the antibodies are selected from the group consisting of polyclonal antibodies and monoclonal antibodies.
- 14. A method of purifying signaling antibodies comprising:
providing an impure antibody composition; contacting the impure antibody composition ECM-complex-chromatography-medium to bind antibodies to the ECM complex; and eluting the bound antibodies from the ECM-complex-chromatography-medium.
- 15. The method as recited in claim 14 wherein the method further comprises contacting the antibodies eluted from the ECM-complex-chromatography-medium with normal-serum-chromatography-medium to bind contaminants to the normal-serum-chromatography-medium.
- 16. The method as recited in claim 14 wherein the method further comprises contacting the antibodies eluted from the ECM-complex-chromatography-medium with normal-plasma-chromatography-medium to bind contaminants to the normal-plasma-chromatography-medium.
- 17. The method as recited in claim 14 wherein the method further comprises contacting the antibodies eluted from the ECM-complex-chromatography-medium with type-IV-collagen-chromatography-medium to bind contaminants to the type-IV-collagen-chromatography-medium.
- 18. A method as recited in claim 14 wherein the antibodies are selected from the group consisting of polyclonal antibodies and monoclonal antibodies.
- 19. A method as recited in claim 14 wherein the method further comprises labeling the antibodies.
- 20. The method as recited in claim 19 wherein the label is selected from the group consisting of enzyme labels, biotin labels, fluorescent labels and radioactive labels.
Parent Case Info
[0001] This is a continuation of pending patent application Ser. No. 08/229,290, filed Apr. 18, 1994.
Continuations (2)
|
Number |
Date |
Country |
Parent |
08325659 |
Oct 1994 |
US |
Child |
10243097 |
Sep 2002 |
US |
Parent |
08229290 |
Apr 1994 |
US |
Child |
08325659 |
Oct 1994 |
US |