Claims
- 1. Seroreactive regions on the E1 protein of human papillomavirus (HPV) 16, with the following amino-acid sequences:
- 2. Seroreactive regions on the E2 protein of human papillomavirus (HPV) 16, with the following amino-acid sequences:
- 3. A peptide which contains one or more of the seroreactive regions as claimed in claim 1.
- 4. A peptide which contains one or more of the seroreactive regions as claimed in claim 2.
- 5. A vaccine which contains one or more of the peptides as claimed in claim 3.
- 6. A vaccine which contains one or more of the peptides as claimed in claim 4.
- 7. A composition for diagnostic purposes for identifying specific antibodies against HPV 16 E1 or E2 protein, which contains peptides as claimed in claim 3.
- 8. A composition for diagnostic purposes for identifying specific antibodies against HPV 16 E1 or E2 protein, which contains peptides as claimed in claim 4.
- 9. A monoclonal antibody which has affinity for the seroreactive regions of claim 1.
- 10. A monoclonal antibody which has affinity for the seroreactive regions of claim 2.
- 11. A composition for diagnostic purposes, which contains a monoclonal antibody as claimed in claim 9.
- 12. A composition for diagnostic purposes, which contains a monoclonal antibody as claimed in claim 10.
- 13. A composition for diagnostic purposes as claimed in claim 11 for identifying HPV 16 specific E1 or E2 proteins.
- 14. A composition for diagnostic purposes as claimed in claim 12 for identifying HPV 16 specific E1 or E2 proteins.
- 15. The use of peptides as claimed in claim 3 for producing vaccine or for compositions for diagnostic purposes.
- 16. The use of peptides as claimed in claim 4 for producing vaccine or for compositions for diagnostic purposes.
- 17. An antibody that binds to an amino acid sequence present in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, or SEQ ID NO: 22.
- 18. The antibody of claim 17, wherein the antibody detects or identifies the E1 or E2 protein of human papilloma virus 16 (HPV 16).
- 19. The antibody of claim 17, which is a monoclonal antibody.
- 20. The antibody of claim 17, which is a polyclonal antibody.
- 21. Polyclonal antisera comprising the antibody of claim 17.
- 22. A composition comprising the antibody of claim 17.
- 23. The composition of claim 22, which is a diagnostic composition.
- 24. The composition of claim 22, wherein the composition is used for identifying, diagnosing, or detecting HPV 16 infection.
- 25. A method of identifying, diagnosing, or detecting HPV 16 infection, said method comprising
contacting a sample from a human suspected of being infected with HPV 16 with an antibody that binds to an amino acid sequence present in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, or SEQ ID NO: 22, and detecting binding of said antibody to said amino acid sequence, wherein binding indicates an HPV 16 infection.
- 26. The method of claim 25, wherein said detecting is by enzyme linked immunosorbent assay (ELISA).
- 27. The method of claim 25, wherein said method detects binding of said antibody with the E1a, E1b, or E2 protein of HPV 16.
Priority Claims (1)
Number |
Date |
Country |
Kind |
P 41 23 760.9 |
Jul 1991 |
DE |
|
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a divisional of U.S. application Ser. No. 09/758,318, filed Jan. 12, 2001, now allowed; which is a divisional of U.S. application Ser. No. 08/468,337, filed Jun. 6, 1995, now U.S. Pat. No. 6,221,577; which is a divisional of U.S. application Ser. No. 08/237,418, filed May 3, 1994, now U.S. Pat. No. 5,601,973; which is a continuation of application Ser. No. 07/913,613, filed Jul. 16, 1992, now abandoned. Applicants rely on the disclosures, and claim the benefit of the filing date, of all of these applications.
Divisions (3)
|
Number |
Date |
Country |
Parent |
09758318 |
Jan 2001 |
US |
Child |
10340783 |
Jan 2003 |
US |
Parent |
08468337 |
Jun 1995 |
US |
Child |
09758318 |
Jan 2001 |
US |
Parent |
08237418 |
May 1994 |
US |
Child |
08468337 |
Jun 1995 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
07913613 |
Jul 1992 |
US |
Child |
08237418 |
May 1994 |
US |