ANTIBODY CHIP, ANTIGEN MEASURING APPARATUS AND LIQUID DISCHARGING METHOD

Abstract

It is an object to provide an antibody chip and a light source measuring apparatus which are excellent in a reproducibility and a workability of a measurement and can easily be utilized. A light source measuring apparatus (1) includes, as a main structure, a concentration measuring device (2), an antibody chip (3) and a cell washing device (4). The concentration measuring device (2) serves to measure a concentration of an antigen in a specimen solution taken by the antibody chip (3) The cell washing device (4) has solution injecting means constituted by an injecting pump (44), an injecting tube (45), a first solution vessel (46), a second solution vessel (47) and a solution selecting valve (48) and solution discharging means constituted by a plurality of discharging pumps (51), a discharging tube (52), an intake end (52a) and the like. In a state in which a container cover (8) is opened, an inner part of a cell (39) of the antibody chip (3) having an antibody fixing layer formed on a main surface of a substrate (37) on a chip carrier (7) is washed to stabilize the antibody fixing layer without the solution remaining in the cell.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a schematic view showing a light source measuring apparatus according to an embodiment of the present invention,

FIG. 2 is a top view showing an antibody chip according to the embodiment of the present invention,

FIG. 3 is a view taken along a Y-Y line in FIG. 2,

FIGS. 4A to 4D are top views showing a cell of the antibody chip according to the embodiment of the present invention,

FIGS. 5A to 5D are top views showing another cell of the antibody chip according to the embodiment of the present invention,

FIGS. 6A to 6D are longitudinal sectional views showing a tip of a discharging tube according to the embodiment of the present invention,

FIGS. 7A to 7D are longitudinal sectional views showing a tip of another discharging tube according to the embodiment of the present invention,

FIGS. 8A to 8D are longitudinal sectional views showing a tip of a further discharging tube according to the embodiment of the present invention,

FIG. 9 is a flowchart showing a process for an antigen measuring method using an antibody chip according to the embodiment of the present invention,

FIGS. 10A to 10D are explanatory views showing the antigen measuring method using an antibody chip according to the embodiment of the present invention, and

FIGS. 11A and 11B are sectional views showing a method of injecting/discharging a solution in the cell of the antibody chip according to the embodiment of the present invention.

Claims

1. An antibody chip comprising: a substrate having a light transmitting property;an antibody fixing layer formed on a main surface of the substrate;a pair of optical elements for causing a light to be incident in the substrate, irradiating the light on the underneath of the antibody fixing layer and then emitting the light to an outside of the substrate;a water-repellent layer formed on the main surface of the substrate in order to surround the antibody fixing layer; anda frame having one of ends which is fixed to the main surface of the substrate and serving to form a cell in order to surround the water-repellent layer,wherein a surface of the substrate surrounded by the frame is constituted by the antibody fixing layer and the water-repellent layer region.

2. An antibody chip comprising: a substrate having a light transmitting property;an antibody fixing layer formed on a main surface of the substrate;a pair of optical elements for causing a light to be incident in the substrate, irradiating the light on the underneath of the antibody fixing layer and then emitting the light to an outside of the substrate;a water-repellent layer formed on the main surface of the substrate in order to surround the antibody fixing layer; anda frame having one of ends which is fixed to the main surface of the substrate and serving to form a cell in order to surround the water-repellent layer, an internal wall of the cell formed by the frame being constituted by a cylindrical portion and a corner portion or a curved portion having a smaller radius of curvature than a radius of curvature of the cylindrical portion,wherein a surface of the substrate surrounded by the frame is constituted by the antibody fixing layer and the water-repellent layer region.

3. An antibody chip comprising: a substrate having a light transmitting property;an antibody fixing layer formed on a main surface of the substrate;a pair of optical elements for causing a light to be incident in the substrate, irradiating the light on the underneath of the antibody fixing layer and then emitting the light to an outside of the substrate;a water-repellent layer formed on the main surface of the substrate in order to surround the antibody fixing layer; anda frame having one of ends which is fixed to the main surface of the substrate and serving to form a cell in order to surround the water-repellent layer, an internal wall of the cell formed by the frame being constituted by a plane portion and a curved portion,wherein a surface of the substrate surrounded by the frame is constituted by the antibody fixing layer and the water-repellent layer region.

4. The antibody chip according to claim 1, wherein the frame is constituted by a light shielding acrylonitrile-butadiene-styrene resin or an acrylic resin.

5. The antibody chip according to claim 1, wherein the water-repellent layer is constituted by a fluorinated resin material having a light shielding property.

6. An antigen measuring apparatus comprising an antibody chip including a substrate having a light transmitting property, an antibody fixing layer formed on one of main surfaces of the substrate, a pair of optical elements for causing a light to be incident in the substrate, irradiating the light on the underneath of the antibody fixing layer and then emitting the light to an outside of the substrate, and a frame having one of ends which is fixed to one of the main surfaces of the substrate and surrounding the antibody fixing layer to form a cell; a light emitting element for causing the light to be incident toward a pair of the optical elements which is provided on an incident side from the other main surface of the substrate;a light receiving element for detecting the light emitted from the other main surface of the substrate through the pair of the optical elements on an emitting side; andsolution injecting means for injecting a predetermined solution into the cell of the antibody chip and solution discharging means for discharging the solution to an outside of the cell,wherein a discharging tube constituting the solution discharging means and serving to discharge the solution in the cell is formed to freely move into/out of the cell.

7. The antigen measuring apparatus according to claim 6, wherein a tip surface of the discharging tube is planar and is disposed in parallel with one of the main surfaces of the substrate when it is caused to enter the cell and is fixed.

8. The antigen measuring apparatus according to claim 6, wherein a tip of the discharging tube is cylindrical and has a smaller outside diameter than a diameter of an internal wall of the cell in a range of 0.02 mm to 10 mm.

9. A method of discharging a liquid from a cell of an antibody chip, the antibody chip comprising: a substrate having a light transmitting property;an antibody fixing layer formed on a main surface of the substrate;a pair of optical elements for causing a light to be incident in the substrate, irradiating the light on the underneath of the antibody fixing layer and then emitting the light to an outside of the substrate;a water-repellent layer formed on the main surface of the substrate in order to surround the antibody fixing layer; anda frame having one of ends which is fixed to the main surface of the substrate and serving to form the cylindrical cell in order to surround the water-repellent layer,a surface of the substrate surrounded by the frame being constituted by the antibody fixing layer and the water-repellent layer region,wherein a discharging tube is disposed in a central part of the cylindrical cell in order not to come in contact with the substrate and a pressure reducing apparatus connected to the discharging tube is driven to discharge the liquid present in the cell.

10. A method of discharging a liquid from a cell of an antibody chip, the antibody chip comprising: a substrate having a light transmitting property;an antibody fixing layer formed on a main surface of the substrate;a pair of optical elements for causing a light to be incident in the substrate, irradiating the light on the underneath of the antibody fixing layer and then emitting the light to an outside of the substrate;a water-repellent layer formed on the main surface of the substrate in order to surround the antibody fixing layer; anda frame having one of ends which is fixed to the main surface of the substrate and serving to form a cell wall surface by a plane portion or a first curved portion and a second curved portion having a smaller radius of curvature than the curved portion or a corner portion in order to surround the water-repellent layer,a surface of the substrate surrounded by the frame being constituted by the antibody fixing layer and the water-repellent layer region,wherein a discharging tube is disposed in the second curved portion or the corner portion of the cell in order not to come in contact with the substrate and a pressure reducing apparatus connected to the discharging tube is driven to discharge the liquid present in the cell.