Antibody Encapsulated Electrochemical Biosensor for GABA

Information

  • Research Project
  • 6643093
  • ApplicationId
    6643093
  • Core Project Number
    R43NS046088
  • Full Project Number
    1R43NS046088-01
  • Serial Number
    46088
  • FOA Number
    PA-02-25
  • Sub Project Id
  • Project Start Date
    6/1/2003 - 21 years ago
  • Project End Date
    5/31/2005 - 19 years ago
  • Program Officer Name
    GRABB, MARGARET C.
  • Budget Start Date
    6/1/2003 - 21 years ago
  • Budget End Date
    5/31/2004 - 20 years ago
  • Fiscal Year
    2003
  • Support Year
    1
  • Suffix
  • Award Notice Date
    5/26/2003 - 21 years ago
Organizations

Antibody Encapsulated Electrochemical Biosensor for GABA

[unreadable] DESCRIPTION (provided by applicant): Gamma-aminobutyric acid ("GABA") is a main mammalian nervous system inhibitory neurotransmitter, playing important roles in neural function and dysfunction. Accurate real time measurement of GABA will greatly accelerate discoveries on the key role of GABA in motor disorders including Huntington's disease and Parkinson's, seizures, myoclonic discharges, and alcohol addiction. Current technology for extracellular measurement of GABA focuses on microdialysis of the cerebro-spinal fluid, followed by liquid chromatography combined with pre-/post column derivatization. Liquid chromatography-based measurements are not continuous, while microdialysis is an invasive procedure causing neuronal death and reactive gliosis with poor spatial-temporal resolution. INFRAMAT proposes to demonstrate feasibility of exploiting functionalized biomaterials to dramatically improve biosensor performance in high sensitivity GABA detection, via a "competitive enzyme immunoassay" strategy, electrochemically measuring non-electroactive GABA neurotransmitters. The proposed technology will adopt IMC's wet chemical synthesis to produce antibody-linked nanoparticles, immobilize functionalized nanoparticles onto transducers to obtain high sensitivity biosensors for real time monitoring GABA concentrations, and enable in-vitro testing of the biosensor based functionalized nanoparticles in artificial cerebro-spinal fluid. This nano-engineered approach will enable biosensors for continuous and direct measurement of GABA concentrations, and can be extended to multiple channels to obtain a spatial-temporal distribution in brain slice and cell culture preparations. [unreadable] [unreadable] [unreadable]

IC Name
NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
  • Activity
    R43
  • Administering IC
    NS
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
    293730
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    853
  • Ed Inst. Type
  • Funding ICs
    NINDS:293730\
  • Funding Mechanism
  • Study Section
    ZRG1
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    INFRAMAT CORPORATION
  • Organization Department
  • Organization DUNS
  • Organization City
    FARMINGTON
  • Organization State
    CT
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    060322597
  • Organization District
    UNITED STATES