Claims
- 1. A purified human antibody which binds to stem cell factor protein, said antibody comprising
a light chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOS: 19-24 or a heavy chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOS: 13-18, said antibody being optionally bound to a cytotoxic molecule or detectable label.
- 2. The antibody of claim 1 wherein said antibody comprises heavy and light chain variable regions comprising a pair of amino acid sequences selected from the group consisting of SEQ ID NOS: 13 and 19, SEQ ID NOS: 14 and 20, SEQ ID NOS: 15 and 24, SEQ ID NOS: 16 and 21, SEQ ID NOS: 17 and 22, and SEQ ID NOS: 18 and 23.
- 3. The antibody of claim 1 wherein said heavy chain variable region comprises human VH3 consensus framework residues.
- 4. The antibody of claim 1 wherein said light chain variable region comprises human Vκ1 consensus framework residues.
- 5. The antibody of claim 1 wherein said light chain variable region comprises human Vλ1 consensus framework residues.
- 6. The antibody of claim 2 wherein said antibody comprises heavy and light chain variable regions comprising the pair of amino acid sequences SEQ ID NOS: 14 and 20.
- 7. The antibody of claim 6 wherein said antibody comprises a light chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOS: 57, 59, 61, 63 or 65 and a light chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOS: 67, 69, 71, 73 and 75.
- 8. The antibody of claim 7 wherein said antibody comprises a heavy chain variable region comprising SEQ ID NO: 14, a light chain variable region comprising SEQ ID NO: 20, a light chain variable region comprising SEQ ID NO: 59, and a light chain variable region comprising SEQ ID NO: 69.
- 9. The antibody of claim 8 wherein said antibody comprises heavy chain variable and constant regions having the amino acid sequence of SEQ ID NO: 77, and light chain variable and constant regions having the amino acid sequence of SEQ ID NO: 79.
- 10. The antibody of claim 1 wherein said antibody is a full-length antibody.
- 11. The antibody of claim 1 wherein said antibody is an IgG.
- 12. The antibody of claim 1 wherein said antibody is an antibody fragment.
- 13. A preparation comprising the antibody of claim 1.
- 14. A pharmaceutical composition comprising the antibody of claim 1 and a pharmaceutically acceptable carrier.
- 15. An isolated polynucleotide or nucleotides encoding the antibody of claim 1.
- 16. An expression vector comprising the polynucleotide or polynucleotides of claim 15.
- 17. A host cell comprising the expression vector of claim 16.
- 18. A method of producing a human antibody, comprising the steps of: culturing a host cell of claim 17 under conditions whereby the antibody is expressed; and purifying the antibody from the host cell or host cell culture medium.
- 19. A method of treating asthma comprising the steps of administering to a human in need of such treatment an effective amount of a Human Stem Cell Factor antibody of claim 1.
- 20. A method of treating a human disorder in which stem cell factor protein is expressed in certain cells, comprising the steps of administering to a human in need of such treatment an effective amount of a human stem cell factor antibody of claim 1 wherein said antibody is bound to a cytotoxic molecule, which cytotoxic molecule is capable of inducing apoptosis in said stem cell factor expressing cells.
- 21. A method for identifying a disorder in which stem cell factor protein level is elevated, comprising the steps of: contacting a sample from a patient suspected of having the disorder with an antibody of claim 1, and detecting the presence of an antibody-stem cell factor protein complex, whereby the presence of an amount of the complex which is greater than the amount of the complex in a normal sample identifies the patient as likely to have the disorder.
- 22. The method of claim 21 wherein said antibody of claim 1 is bound to a detectable label.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional Application 60/342,174 filed Dec. 17, 2001, which is hereby incorporated herein in its entirety by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60342174 |
Dec 2001 |
US |