Patent Application
20140178854
The disclosure relates to methods of detecting MLV related viruses, including retroviral replicating vectors, and detecting immune responses to such viruses using purified whole viral particles as the target antigen by ELISA, competitive binding, Western blot or other serological detection methods. The purified particles can also be used for stimulation of T cells to detect cellular immune response.
There are several needs and advantages that come from the ability to detect the presence of MLV or MLV related viruses in human or mammalian subjects. Firstly therapies are currently being developed that employ retroviral replicating vectors (RRV) in human therapies, mainly for cancer (see WO2010036986 and (www).clinicaltrials.gov NCT01156584) and there is a need to follow the RRV replication in the blood stream in case this reaches levels considered potentially pathogenic and warrant pharmacological intervention (e.g., around several hundred thousand copies/ml for HIV). Quantitative or semi-quantitative seropositivity has been a useful way to follow ongoing viral infections in general, and retroviral infection in particular, and provides information on infection and active replication of such agents. However the specifics of individual RRV's may vary and it is possible that changes in the virus may occur after prolonged replication in humans or animals. Therefore it is important that an assay for monitoring such events be as broad-based as possible and not focus on one or a few antigenic epitopes. Secondly, it seems very likely that some individuals may be or have been infected with MLV related viruses. This is based on the observation that high level seropositivity to MLV related viruse has been clearly shown to exist in a small percentage of individuals (around 1%). Recently various publications suggest that several exogenous gammaretroviruses have been isolated from humans, and these are known as XMRV or MLV-related viruses (MLVRV, Urisman A, Molinaro R J, et al. (2006) PLoS Pathog 2(3): e25., Lombardi V. c. et al. Science 2009, Lo et al PNAS 2010 (www).pnas.org/cgi/doi/10.1073/pnas.1006901107).
The disclosure provides a method for detecting anti-viral antibodies for amphotropic MLV or MLV-like virus in a biological sample compromising (a) incubating a biological sample with a capture reagent immobilized on a solid support to bind multiple anti-viral antibodies to amphotropic MLV to the capture reagent, wherein the capture reagent comprises a purified viral preparation; and (b) detecting bound anti-viral antibodies bound to immobilized capture reagent by contacting the bound anti-viral antibodies with a labeled detecting secondary agent. In one embodiment, the biological sample is isolated from a mammal. In further embodiment, the mammal is an autoimmune, virally infected, immune suppressed or cancer patient. In yet another embodiment, the biological sample is isolated from a human. In a further embodiment, the human is an autoimmune, virally infected immune suppression or cancer patient. In one embodiment, the method further comprises measuring the amount of anti-viral antibody, wherein the amount of antibody is titred using a dilution series; wherein the amount of antibody determined is IgG or IgM by use of specific detecting secondary reagents. In one embodiment, the biological sample is plasma, serum, urine, saliva, bucchal swab, nasal swab, fine needle aspirate, semen, vaginal secretion or milk. In yet another embodiment, the capture reagent is immobilized at about 1.5 μg/mL. In one embodiment, the solid support is a microtiter plate, a glass slide, a nylon slide, a nitrocellulose membrane or a PVDF membrane. In another embodiment, the solid support is a latex bead, a glass bead or a microsphere. In a further embodiment, the solid support is a pipette tip, microfluidic card or semiconductor chip. In yet another embodiment, the secondary agent is a secondary antibody that is detectably labeled. In certain embodiment, the method further comprises a control comprising the monoclonal 83A25 mAb. In a further embodiment, the method further comprises a control comprising human serum or plasma from an individual who is strongly seropositive for MLV, identified by screening multiple human sera.
The disclosure also provides an immunoassay kit for detecting anti-viral antibodies comprising: (a) a monoclonal antibody that specifically binds to amphotropic envelop as a control; and (b) a capture reagent comprising a purified viral particle. In one embodiment, the solid support is a microtiter plate. In a further embodiment, the solid support is a microsphere. In yet another embodiment, the capture reagent is an MLV-like viral particle. In yet another embodiment, the capture reagent is a purified protein of MLV origin. In yet another embodiment, the capture reagent is a purified recombinant protein of MLV origin or MLV-like sequence. In another embodiment, the positive control is human serum or plasma from an individual who is strongly seropositive to the capture antigen, and such serum has been identified by screening multiple human sera. In one embodiment, the detectable antibody is a monoclonal antibody. In yet a further embodiment, the detectable monoclonal antibody is a murine monoclonal antibody. In yet another embodiment, the detectable antibody is a polyclonal antibody. In a further embodiment, the detectable polyclonal antibody is a rabbit or goat or sheep or chicken polyclonal antibody. In any of the foregoing embodiments, the kit is used in a clinical or diagnostic setting. In one embodiment, the kit is used to monitor the immune responses of MLV-infected or MLV-like infected or treated individuals.
In any of the foregoing methods or kits the purified viral particle is produced by a method comprising transforming a 293 cell line with a plasmid encoding a retroviral MLV or MVL-like virus; culturing the 293 cell to produce viral particles; isolating the viral particles; infecting an HT1080 cell line with the viral particles thereby producing the viral particle producing cell line, producing the viral particles and purifying the viral particles.
The disclosure provides retrovirus producing cell lines for the production of a replication competent retrovirus particles, the cell line comprising a mammalian cell line such as a prostate cancer cell, a T cell line, a neural tumor line, a fibrosarcoma, an osteosarcoma a thymoma cell line, a fibroblast line or endothelial cell line, said cell line stably expressing a retroviral genome comprising a gag gene, pol gene, env gene, and retroviral psi (Ψ) factor for the assembly of the recombinant retroviral genome. In one embodiment, the replication competent retrovirus particle is stably expressed. In another embodiment the immortalized cell line is a human cell line. In another embodiment the cell line is selected from one of: the prostate tumor cell lines LNCAP (ATCC#CRL-1740, DU145 (ATCC#HTB-81), or PC-3 (ATCC#CRL-1435); the human fibrosarcoma cell line HT1080 (ATCC#CCL-121); the Human glioma cell line U87-MG (ATCC#HTB-14); the human T cell line SupT1 cell line (ATCC#CRL-1942); the human cervical carcinoma line Hela, the african green monkey cell line CV1 or derivatives such as COS cells; HEK 293 and 293 derived cells; or CHO and CHO derived cells. In another embodiment the cell line was isolated and already expressed the retrovirus. In a related embodiment the cell line is 222Rv1 (ATCC CRL-2505, Knouf et al. J. Virol 83: 7353-7356, 2009) which makes the artifactual recombinant XMRV (T. Paprotka, et al. 2011, op.cit.). In another embodiment, the half life is greater than 7 days at 2-8° C. In one embodiment, the retroviral polynucleotide sequence is derived from one of murine leukemia virus (MLV), Moloney murine leukemia virus (MoMLV), amphotropic MoMLV, Xenotropic MLV related retrovirus (XMRV), MLV related virus (MLVRV) found in humans, xenotropic MLV, Polytropic MLV, amphotropic MLV, Gibbon ape leukemia virus (GALV), murine mammary tumor virus (MuMTV), Rous Sarcoma Virus (RSV), Gibbon ape leukemia virus (GALV), baboon endogenous virus (BEV), feline virus RD114, T5.0002 (WO2010045002A2) or other gamma retrovirus, or hybrids of the above. The genome of the retrovirus produced by the producer cell line or introduced into a cell line to make a producer cell line comprises various domains. For example, the promoter may comprise a CMV promoter having a sequence as set forth in SEQ ID NO:7, 8 or 9 from nucleotide 1 to about nucleotide 582 and may include modification to one or more nucleic acid bases and which is capable of directing and initiating transcription; a CMV-R-U5 domain polynucleotide comprises a sequence as set forth in SEQ ID NO: 7, 8 or 9 from about nucleotide 1 to about nucleotide 1202 or sequences that are at least 95% identical to a sequence as set forth in SEQ ID NO: 7, 8 or 9, wherein the polynucleotide promotes transcription of a nucleic acid molecule operably linked thereto; the gag nucleic acid domain comprises a sequence from about nucleotide number 1203 to about nucleotide 2819 of SEQ ID NO: 7 or 9 or a sequence having at least 60%, 70% 80%, 90%, 95%, 98%, 99% or 99.8% identity thereto; the pol domain comprises a sequence from about nucleotide number 2820 to about nucleotide 6358 of SEQ ID NO:7 or 9 or a sequence having at least 60%, 70% 80%, 90%, 95%, 98%, 99% or 99.8% identity thereto; the env domain comprises a sequence from about nucleotide number 6359 to about nucleotide 8323 of SEQ ID NO:7 or 9 or a sequence having at least 60%, 70% 80%, 90%, 95%, 98%, 99% or 99.8% identity thereto; the IRES and the heterologous nucleic acid are optional extra sequences that are used when the virus is being used as a vector.
The disclosure also provides a cell free preparation comprising purified viral particles obtained from the retrovirus producing cell line described herein. In some embodiments a preparation the isolated viral particles is used to make an ELISA or other antibody binding assay for testing human or animal blood, plasma, serum, urine, saliva, bucchal swab, nasal swab, fine needle aspirate, semen, vaginal secretion, milk or other bodily fluid or extract. Antibodies detected can be IgM, IgG, IgA, IgE or IgD, depending on the type of secondary antibody or other secondary binding reagent used in the assay.
The disclosure also provides a method of constructing a vector producing cell line described herein comprising transforming a 293 cell line with a plasmid encoding a retroviral vector comprising from 5′ to 3′: a CMV-R-U5 fusion of the immediate early promoter from human cytomegalovirus to an MLV R-U5 region; a PBS, primer binding site for reverse transcriptase; a 5′ splice site; ψ packaging signal; a gag coding sequence for MLV group specific antigen; a pol coding sequence for MLV polymerase polyprotein; a 3′ splice site; an env coding sequence for envelope protein; culturing the 293 cell to produce viral particles; isolating the viral particles; infecting an cell line with the viral particles thereby producing the viral particle producing cell line. The disclosure also provides plasmids where the promoter is not a CMV-R-U5 fusion of the immediate early promoter from human cytomegalovirus to an MLV R-U5 region but is a fusion of the MLV r-U5 region with and alternate promoter such as the RSV promoter or the actin promoter, or the intact MLV LTR with U3, R and U5 regions. The disclosure also provides cell lines generated by the foregoing methods. The invention also provides virus producing lines, made by these methods with HT1080 cells, PC3 cells, LNCaPcells, SupT1 cells, U87 cells, D17 cells, CF2 cells, 293 cells, Hela cells, CV1 cells, CHO cells or cell lines derived from any of these.
The disclosure also provides a method for producing a composition for testing for anti-retrovirus antibodies comprising culturing the cell lines described herein to produce viral particles and substantially purifying the viral particles.
The disclosure also provides cell banks comprising the cell lines of the disclosure. In some embodiments, the cell lines of the disclosure are grown in suspension. In some embodiment, the cell lines of the disclosure are grown in serum free medium. In some embodiment, the cell line is grown in suspension in serum free medium.
The details of one or more embodiments of the disclosure are set forth in the accompanying drawings and the description below. Other features, objects, and advantages of the disclosure will be apparent from the description and drawings, and from the claims.
As used herein and in the appended claims, the singular forms “a,” “and,” and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to “a cell” includes a plurality of such cells and reference to “the vector” includes reference to one or more vectors, and so forth.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although any methods and reagents similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, the exemplary methods and materials are now described.
Also, the use of “or” means “and/or” unless stated otherwise. Similarly, “comprise,” “comprises,” “comprising” “include,” “includes,” and “including” are interchangeable and not intended to be limiting.
It is to be further understood that where descriptions of various embodiments use the term “comprising,” those skilled in the art would understand that in some specific instances, an embodiment can be alternatively described using language “consisting essentially of” or “consisting of.”
All publications mentioned herein are incorporated herein by reference in full for the purpose of describing and disclosing the methodologies, which are described in the publications, which might be used in connection with the description herein. The publications discussed above and throughout the text are provided solely for their disclosure prior to the filing date of the present application. Nothing herein is to be construed as an admission that the inventors are not entitled to antedate such disclosure by virtue of prior disclosure.
Retroviruses have been classified in various ways but the nomenclature has been standardized in the last decade (see ICTVdB—The Universal Virus Database, v 4 on the World Wide Web (www) at ncbi.nlm.nih.gov/ICTVdb/ICTVdB/ and the text book “Retroviruses,” Eds. Coffin, Hughs and Varmus, Cold Spring Harbor Press 1997; the disclosure of which are incorporated herein by reference). A replication competent retrovirus is derived from the Retroviridae family of viruses and can comprise a member of the Orthoretrovirinae sub-family, or more typically comprises a retrovirus from the gammaretrovirus genus. In one embodiment, the virus comprises a replication competent retrovirus which is a retroviral replicating vector (RRV), which, in turn, comprises an internal ribosomal entry site (IRES) 5′ to a polynucleotide encoding a cytosine deaminase. In one embodiment, the polynucleotide encoding a cytosine deaminase is 3′ to an env polynucleotide of a retroviral vector.
The disclosure provides retroviruses of many kinds as immune reactivity reagents. The retrovirus used in the disclosure can be wild-type or modified retroviruses derived from various members of family. The classification of the retroviridae family has changed several times over the last ten to fifteen years. Currently the Retroviridae family consists of two sub-families: the Spumaretrovirinae-which has a single genus, the spumavirus (or foamy viruses) such as the human and simian foamy virus (HFV) and the Orthoretroviriniae sub-family which has 6 genus-betaretrovirus (e.g., MMTV), gammaretrovirus (e.g., MLV), alpharetrovirus (e.g., ALV) deltaretrovirus (e.g., BLV and HTLV-1) lentivirus (e.g., HIV 1) and epsilon retrovirus (e.g., wall eye dermal sarcoma virus). These classifications are made on the basis of common molecular features such as the relative reading frames for gag, pol and env, the processing of the polyproteins, the individual tRNAS used for priming reverse transcription, and the nature of the LTR structures. The original method of classification of retroviruses was into groups A, B, C and D on the basis of particle morphology, as seen under the electron microscope during viral maturation. A-type particles represent the immature particles of the B- and D-type viruses seen in the cytoplasm of infected cells. These particles are not infectious. B-type particles bud as mature virion from the plasma membrane by the enveloping of intracytoplasmic A-type particles. At the membrane they possess a toroidal core of 75 nm, from which long glycoprotein spikes project. After budding, B-type particles contain an eccentrically located, electron-dense core. The betaretrovirus, Mouse mammary tumor virus (MMTV) has a B-type morphology, but betaretroviruses can also have a D-type structure. D-type particles resemble B-type particles in that they show as ring-like structures in the infected cell cytoplasm, which bud from the cell surface, but the virion incorporate short surface glycoprotein spikes. The electron-dense cores are also eccentrically located within the particles. Mason Pfizer monkey virus (MPMV), also a betaretrovirus, is the prototype D-type virus. No intracytoplasmic particles can be observed in cells infected by C-type viruses. Instead, mature particles bud directly from the cell surface via a crescent ‘C’-shaped condensation which then closes on itself and is enclosed by the plasma membrane. Envelope glycoprotein spikes may be visible, along with a uniformly electron-dense core. Budding may occur from the surface plasma membrane or directly into intracellular vacuoles. Alpharetroviruses, gammaretroviruses, deltaretroviruses and epsilonretroviruses all have the C-type structural appearance.
Retroviruses are defined by the way in which they replicate their genetic material. During replication the RNA is converted into DNA. Following infection of the cell a double-stranded molecule of DNA is generated from the two molecules of RNA which are carried in the viral particle by the molecular process known as reverse transcription. The DNA form becomes covalently integrated in the host cell genome as a provirus, from which viral RNAs are expressed with the aid of cellular and/or viral factors. The expressed viral RNAs are packaged into particles and released as infectious virion.
The retrovirus particle is composed of two identical RNA molecules. Each wild-type genome has a positive sense, single-stranded RNA molecule, which is capped at the 5′ end and polyadenylated at the 3′ tail. The diploid virus particle contains the two RNA strands complexed with gag proteins, viral enzymes (pol gene products) and host tRNA molecules within a ‘core’ structure of gag proteins. Surrounding and protecting this capsid is a lipid bilayer, derived from host cell membranes and containing viral envelope (env) proteins. The env proteins bind to a cellular receptor for the virus and the particle typically enters the host cell via receptor-mediated endocytosis and/or membrane fusion.
After the outer envelope is shed, the viral RNA is copied into DNA by reverse transcription. This is catalyzed by the reverse transcriptase enzyme encoded by the pol region and uses the host cell tRNA packaged into the virion as a primer for DNA synthesis. In this way the RNA genome is converted into the more complex DNA genome.
The double-stranded linear DNA produced by reverse transcription may, or may not, have to be circularized in the nucleus. The provirus now has two identical repeats at either end, known as the long terminal repeats (LTR). The termini of the two LTR sequences produces the site recognized by a pol product—the integrase protein—which catalyzes integration, such that the provirus is always joined to host DNA two base pairs (bp) from the ends of the LTRs. A duplication of cellular sequences is seen at the ends of both LTRs, reminiscent of the integration pattern of transposable genetic elements. Integration occurs at a large number of sites within the target cell genome, with biases that depend on the type of retrovirus and the type of cell. However, by modifying the integrase component (int) of the pol gene (see below) it is possible to control or partially direct the integration of a retroviral genome.
Transcription, RNA splicing and translation of the integrated viral DNA is mediated by host cell proteins. Variously spliced transcripts are generated. In the case of the human retroviruses HIV-1/2 and HTLV-I/II viral proteins are also used to regulate gene expression. The interplay between cellular and viral factors is a factor in the control of virus latency and the temporal sequence in which viral genes are expressed.
Retroviruses can be transmitted horizontally and vertically. Efficient infectious transmission of retroviruses requires the expression on the target cell of receptors which specifically recognize the viral envelope proteins, although viruses may use receptor-independent, nonspecific routes of entry at low efficiency. In addition, the target cell type must be able to support all stages of the replication cycle after virus has bound and penetrated. Vertical transmission occurs when the viral genome becomes integrated in the germ line of the host. The provirus will then be passed from generation to generation as though it were a cellular gene. Hence endogenous proviruses become established which frequently lie latent, but which can become activated when the host is exposed to appropriate agents.
As mentioned above, the integrated DNA intermediate is referred to as a provirus. As described below, a helper virus is not required for the production of the recombinant retrovirus of the disclosure, since the sequences for encapsidation are provided in the genome thus providing a replication competent retroviral vector for gene delivery or therapy.
The retroviral genome and the proviral DNA of the disclosure have at least three genes: the gag, the pol, and the env, these genes may be flanked by one or two long terminal (LTR) repeat, or in the provirus are flanked by two long terminal repeat (LTR) and sequences containing cis-acting sequences such as psi. The gag gene encodes the internal structural (matrix, capsid, and nucleocapsid) proteins; the pol gene encodes the RNA-directed DNA polymerase (reverse transcriptase), protease and integrase; and the env gene encodes viral envelope glycoproteins. The 5′ and/or 3′ LTRs serve to promote transcription and polyadenylation of the virion RNAs. The LTR contains all other cis-acting sequences necessary for viral replication. All of these components are present in the viral particles of the current disclosure plus a tRNA molecule and viral membrane derived from the host cell that produced the virus. The env gene product is embedded in the viral envelope membrane, along with some cell surface molecules from the producer cell. The envelope gene is quite heavily glycosylated in a characteristic pattern that is fairly standardized but will depend on the producer cell. For these reasons viral particles produced to detect antibodies in humans are usually best made in human cell lines, but it can be advantageous to make the virus in cells of another species such as dog (e.g. D17 or Cf2 cells) to rule out detection of such self proteins, for example in human subjects with cross-reactive auto-immunity. Lentiviruses have additional genes including vif, vpr, tat, rev, vpu, nef, and vpx (in HIV-1, HIV-2 and/or SIV), and some of these can be incorporated into the viral particles (e.g vif and nef).
Adjacent to the 5′ LTR are sequences necessary for reverse transcription of the genome (the tRNA primer binding site) and for efficient encapsidation of viral RNA into particles (the Psi site). If the sequences necessary for encapsidation (or packaging of retroviral RNA into infectious virion) are missing from the viral genome, the result is a cis defect which prevents encapsidation of genomic viral RNA. In a first embodiment, the disclosure provides a recombinant retrovirus capable of infecting a non-dividing cell, a dividing cell, or a cell having a cell proliferative disorder. The recombinant replication competent retrovirus of the disclosure comprises a polynucleotide sequence encoding a viral GAG, a viral POL, a viral ENV, and optionally a heterologous polynucleotide that is expressed after the viral vector infects a target cell, encapsulated within a virion.
Depending upon the intended use of the retroviral vector of the disclosure any number of heterologous polynucleotide or nucleic acid sequences may be inserted into the retroviral vector. Some examples are given in International Application Publication No. WO2010/036986, incorporated herein by reference.
As mentioned elsewhere herein, XMRV itself is thought to be an artifact caused by contamination of samples by mouse DNA and recombination of laboratory Xenotropic isolates (T. Paprotka, et al. “Recombinant Origin of the Retrovirus XMRV” Science 333, 97 (2011); J. Cohen & M. Enserink “False Postive” Science 333: 1694-1701 (2011); G. Simmons et al. “Failure to Confirm XMRV/MLVs in the Blood of Patients with Chronic Fatigue Syndrome: A Multi-Laboratory Study,” (www).sciencexpress.org/10.1126/science.1213841). However, as demonstrated here (see, e.g.,
In addition, antigens synthesized alone, even in mammalian cells, will not incorporate the three dimensional structures of the virus produced in vivo, and can be inappropriately modified or lack appropriate modification. This can lead to false negatives. Recently specific peptide assays have been designed for XMRV (Qiu et al., Retrovirology, 7:68, 2010) and are unlikely to detect all MLV-like variants. Also assays using cell lysates of retrovirally infected cells have also been designed but these use crude unpurified preparations and, unlike the present disclosure, positive samples were not detected (Switzer et al. Retrovirology, 7:57, 2010) probably due to interference and high background.
The life cycle of retroviruses involves a stage when the virus' genetic material is inserted into the genome of a host cell. This step is essential because the inserted viral nucleic acid, the provirus, is replicated through the host cell machinery. As part of this process the RNA genome of the retrovirus is replicated through a double-stranded DNA intermediate prior to insertion into the genome of the host cell. The initial conversion of the viral RNA molecule into a double-stranded DNA (dsDNA) molecule is performed by a reverse-transcriptase. The dsDNA is then integrated into the hose cell genome by an integrase to further be replicated by the host cellular machinery. The reverse transcriptase and the integrase required for the conversion of the RNA into dsDNA and for the integration into the host genome are carried within the viral particle during infection. The proviral DNA is finally transcribed using the host machinery into multiple RNA copies. These RNA molecules are then translated into viral peptides or proteins or integrated into viral particles which are released from the cell into the medium or extracellular milieu.
A retroviral RNA genome usually comprises 6 typical regions leading to the expression of multiple proteins. These region include the gag, pol and env gene sequences associated with a packaging signal, a psi (ψ) signal and flanked by 5′ and/or 3′ long terminal repeats (LTR) regions. The gag gene leads to the expression of the protein components of the nucleoprotein core of the virus, while the pol gene products are involved in the synthesis polynucleotides and recombination. The env gene codes for the envelope components of the retrovirus particle. 5′ and 3′ LTR regions include promoters and assist in the integration of the viral genome into the chromosomal DNA of the host cell. The psi signal refers to the retroviral packaging signal that controls the efficient packaging of the RNA into the viral particle. Because of their ability to form proviruses, retroviruses are also useful to modify the genome of a target or host cell and various modifications have been made to retroviruses for use in gene therapy. Gene therapy using retroviral vectors is generally performed by adding a heterologous polynucleotide to the viral genome which encodes or produces a polypeptide or transcript of interest, packaging the recombinant genome into a viral particle and infecting a target host cell. The target cell will then incorporate the exogenous gene as being a part of a provirus.
Previously defective retroviral vectors have been used as target antigens to look for immune response after administration of non replicative retroviral vectors (Martineau et al. Human Gene Ther. 8:1231-1241, 1997). The use of individual antigens as described above can be useful, but given the variation in types of MLV with which individuals may be infected, the single antigen target approach risks being overly specific, and not detecting a true positive. However, little is reported about the development of replication competent retroviral vector systems. The methods described here allow immune monitoring of replication competent viral vectors and their persistence.
The term “RCR” or “RRV” as used herein is intended to mean a replication-competent retrovirus (RCR) or replicating retrovirus (RRV). A replication-competent virus is a viral particle that has the capacity to replicate by itself in a host cell. Some RRV/RCR can carrying a heterologous gene.
As used herein, the term “RCR plasmid vector” means a plasmid which includes all or part of a retroviral genome including 5′ and 3′ retroviral long-term repeat (LTR) sequences, a packaging signal (ψ), and may include one or more polynucleotides encoding a protein(s) or polypeptide(s) of interest, such as a therapeutic agent or a selectable marker. The term “therapeutic” is used in a generic sense and includes treating agents, prophylactic agents, and replacement agents.
The terms “vector”, “vector construct” and “expression vector” mean the vehicle by which a DNA or RNA sequence (e.g., a foreign gene) can be introduced into a host cell, so as to transform the host and promote expression (e.g., transcription and translation) of the introduced sequence. Vectors typically comprise the DNA of a transmissible agent, into which foreign DNA encoding a protein is inserted by restriction enzyme technology. A common type of vector is a “plasmid”, which generally is a self-contained molecule of double-stranded DNA that can readily accept additional (foreign) DNA and which can readily introduced into a suitable host cell. A large number of vectors, including plasmid and fungal vectors, have been described for replication and/or expression in a variety of eukaryotic and prokaryotic hosts. Non-limiting examples include pKK plasmids (Clonetech), pUC plasmids, pET plasmids (Novagen, Inc., Madison, Wis.), pRSET or pREP plasmids (Invitrogen, San Diego, Calif.), or pMAL plasmids (New England Biolabs, Beverly, Mass.), and many appropriate host cells, using methods disclosed or cited herein or otherwise known to those skilled in the relevant art. Recombinant cloning vectors will often include one or more replication systems for cloning or expression, one or more markers for selection in the host, e.g., antibiotic resistance, and one or more expression cassettes.
The terms “transfecting” or “transfection” as used herein are intended to mean the transfer of at least one exogenous nucleic acid into a cell. The nucleic acid may be RNA, DNA or a combination of both. The exogenous nucleic acid refers to nucleic that is not found as a result of host cell division or host cell multiplication.
The term “virus” as used herein is intended to mean the physical virus or retrovirus particle.
The term “cell line” as used herein refers to cultured cells that can be passed (divided) more than once. The disclosure relates to cell lines that can be passed more than 2 times, up to 200 times, or more and includes any integer therebetween.
The terms “express” and “expression” mean allowing or causing the information in a gene or DNA sequence to become manifest, for example producing a protein by activating the cellular functions involved in transcription and translation of a corresponding gene or DNA sequence. A DNA sequence is expressed in or by a cell to form an “expression product” such as a protein. The expression product itself, e.g. the resulting protein, may also be said to be “expressed” by the cell. A polynucleotide or polypeptide is expressed recombinantly, for example, when it is expressed or produced in a foreign host cell under the control of a foreign or native promoter, or in a native host cell under the control of a foreign promoter.
The terms “stable expression” and “stably expressing” as used herein are intended to mean that the genetic material that is being stably expressed and/or is integrated permanently and stably in the genome of the host cell, and thus has the same expression potential over time as the native genetic material of the host cell.
The terms “transient expression” and “transiently expressing” as used herein are intended to mean that the genetic material temporal expression period and/or is not integrated permanently and stably in the genome of the host cell, and thus does not have the same expression potential over time as the native genetic material of the host cell.
As used herein, the term “heterologous” nucleic acid sequence or transgene refers to (i) a sequence that does not normally exist in a wild-type retrovirus, (ii) a sequence that originates from a foreign species, or (iii) if from the same species, it may be substantially modified from its original form. Alternatively, an unchanged nucleic acid sequence that is not normally expressed in a cell is a heterologous nucleic acid sequence.
The term “therapeutic” as used herein refers to an action that prevents, reverses, or slows the natural course of a disease or disorder, or its symptoms. A therapeutic action can be preventive, curative or merely palliative, and does not mean that the affected human or animal patient will not die from the disease.
The term MLV related virus (MLVRV) refers to a retrovirus that infects either by natural transmission or by intentional administration a species other than rodents and has at least 60% homology to at least one of the LTR, Gag, pol, integrase, envelope or non-coding regions of canonical MLV's (NCBI ((http://www).ncbi.nlm.nih.gov/) listed MLV's such as ecotropic MLV NC—001501.1, amphotropic MLV (AF010170.1), XMRV (FN692043.1), xenotropic MLV, or polytropic MLV).
An antigenic viral particle or antibody-binding viral particle is produced by a producer cell of the disclosure. In one embodiment, the producer cell lines of the disclosure are capable of growth as an adherent culture, in suspension or in a serum-free medium. The producer cell lines can also be grown both in serum-free medium and suspension simultaneously, or as an adherent line in serum free media. Although serum-free medium and the capacity to grow in suspension are the typical conditions, cells of the disclosure (e.g., 293, 293T, HT1080 cells, PC3 cells, LNCaP cells, SupT1 cells, U87 cells, D17 cells or CF2 cells) can be cultured in an adherent manner with regular serum-containing medium to achieve particular purposes. Such purposes can be, for example, to facilitate transfection of cells or to select cell clones.
The type of producer cells used to generate the retrovirus (described more fully below) is useful for the production of replication competent viral particles for use as targets to detect antibody responses to the vector particle or components thereof. Such methods are useful for monitoring infection, tissue sample and gene delivery and gene therapy using retroviral particles.
The disclosure provides a method of generating a producer cell line comprising transforming or transfecting a first mammalian cell type with an RCR plasmid vector of the disclosure, culturing the first cell type to produce retroviral particles, obtaining a cell free media from the first cell type producing the retroviral particles, wherein the cell free media comprises retroviral particles, contacting a second mammalian cell type with the media to infect the second cell type and culturing the second cell type to produce a producer cell line that produces a replication competent retroviral vector for use in transforming mammalian cells. The first cell type can be almost any mammalian cell type that is capable of producing virus after transfection and may include HeLa, COS, Chinese Hamster Ovary (CHO), and HT1080 cells, and the transfection can be with calcium phosphate or other agents such as lipid formulations known to those skilled in the art as useful for transfection.
In one embodiment, the first cell type is a human embryonic kidney cell. In another embodiment, the human embryonic kidney cell is a 293 cell (also often referred to as HEK 293 cells, 293 cells, or less precisely as HEK cells), which are a cell line originally derived from human embryonic kidney cells grown in tissue culture. HEK 293 cells were generated by transformation of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA. HEK 293 cells are easy to grow and transfect very readily and have been widely-used in cell biology research for many years. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy.
In another embodiment, the first cell type is a mammalian cell transformed with an SV40 Large T-antigen. In a particular embodiment, 293T HEK cells are used. An important variant of this cell line is the 293T cell line which contains the SV40 Large T-antigen allowing for episomal replication of transfected plasmids containing the SV40 origin of replication. This allows for amplification of transfected plasmids and extended temporal expression of the desired gene products.
The term “human 293 cell” as used herein includes the HEK 293T cell line, the human 293 cell line (ATCC No. CRL 1573) (Graham, et al., J. Gen. Virol., Vol. 36, pgs. 59-72 (1977)), or a cell line formed by transfecting 293 cells with one or more expression vehicles (e.g., plasmid vectors) including polynucleotides encoding various gag, pol, and env proteins. The envelope may be an amphotropic envelope, an ecotropic envelope, a xenotropic envelope, an XMRV envelope, a polytropic envelope, a GALV envelope, an RD114 envelope, an FeLV envelope or other retroviral envelope. Such cells also may include other polynucleotides such as, for example, polynucleotides encoding selectable markers.
The first cell type (e.g., HEK 293T cells) may be transformed with an RCR plasmid vector of the disclosure in any number of means including calcium phosphate and the like. Typical culture conditions for mammalian cells, particularly human 293 cells are known in the art.
Once transformed the first cell type is cultured under conditions for production of viral particles. Such conditions typically include refeeding cells in appropriate media, CO2, and humidity. The culture conditions may also include the addition of antibiotics, anti-fungals, growth factors and the like. Typically the refed medium is harvested after 24, 48, 72, or 96 hours, and such a procedure is known as a transient expression transfection procedure.
The media from the cultured cells above may be used directly in further culturing. Alternatively, the viral particles in the media of cultured cells may be isolated using any number of techniques known in the art including centrifugation, size exclusion techniques, anion exchange chromatography and the like.
Where the media is used directly, the media can be added to media used in the culture of the second cell type. Where the viral particles are first substantially purified, the particles may be washed or resuspended in an appropriate buffer or media or at particular concentration for infectivity before addition to the second cell type, leading to the generation of a stable expression producer cell line.
In one embodiment, the second cell type is a human fibroscarcoma cell line. In a specific embodiment, the cell line is an HT1080 cell line or a derivative thereof. HT1080 human fibrosarcoma cell line (ATCC, Catalog#CCL-121) can be obtained directly from the American Type Culture Collection (P.O. Box 1549, Manassas, Va.). The method includes infecting the HT1080 cells with an RCR of the disclosure to provide a stably transfected host cell. The stably transfected host cell may be cultured to produce viral particles for use in gene therapy or gene delivery or may be “banked” for later use, and may be a pool of transfected cells, or a cloned cell line. The banked cells may be frozen and stored using techniques known in the art.
In a further embodiment the cells infected with the virus generated by transient transfection or by other means, can be any mammalian cell line known to be infectable with the corresponding virus. Infectability by a virus can be tested by methods described in Example 1 and shown in
In one embodiment, the cells are cultured in the presence of fetal calf serum or other non-defined additives. In another embodiment, the cells will be cultured in serum-free media. In one embodiment, the cells are culture in an animal free media or a defined media. RCR viral particles (e.g., MLV related virus) can be substantially purified from the media of the producer cells. The purified virus can be washed, diluted and resuspended in an appropriate pharmaceutically acceptable carrier. Alternatively, the purified vector may be stored either by freezing of lypholization.
In one embodiment, the virus will be supplied as an aqueous sterile solution containing the following formulation excipients (in mg/mL): sucrose 10.0, mannitol 10.0, and NaCl 5.3. The solution may also contain Human Serum Albumin (HSA, Baxter) 1.0 and ascorbic acid 0.10.
As described further herein, any number of retroviruses of the disclosure may be used with the producer cell lines and process described herein.
In specific examples provided herein, Toca 511 an amphotropic MLV that carries a heterologous polynucleotide encoding a polypeptide with cytosine deaminase (see, e.g., WO2010036986 A2, incorporated herein by reference) is used to demonstrate the methods and compositions of the disclosure. In another specific example, amphotropic MLV (ATCC VR-1450) is used to demonstrate the methods and compositions of the disclosure. As described herein, Toca 511 refers to a replication competent retroviral vector encoded in a plasmid designated as pAC3-yCD2 and Ampho MLV refers to a retrovirus encoded in a plasmid designated pAMS, ATCC #45167. The virus is comprised of a replication-competent retrovirus derived from a murine leukemia virus (MLV) encoding all retroviral components (gag, pol and env) required for viral replication, with the original ecotropic envelope replaced with the amphotropic envelope from the 4070A virus. Alternatively, other virus encoding plasmids or viral preparations can be used, e.g., XMRV, or MV related virus.
The methods and compositions of the disclosure are applicable to other viruses, viral vectors and recombinant retroviral vectors. The disclosure describes various modification and recombinant vectors that can be produced by the cell lines and methods of the disclosure.
The retrovirus and methods of the disclosure provide a replication competent retrovirus that does not require helper virus or additional nucleic acid sequence or proteins in order to propagate and produce virion. For example, the nucleic acid sequences of the retrovirus of the disclosure encode, for example, a group specific antigen and reverse transcriptase, (and integrase and protease-enzymes necessary for maturation and reverse transcription), respectively, as discussed above. The viral gag and pol can be derived from a lentivirus, such as HIV or a gammaretrovirus such as MoMLV. In addition, the nucleic acid genome of the retrovirus of the disclosure includes a sequence encoding a viral envelope (ENV) protein. The env gene can be derived from any retroviruses. The env may be an amphotropic envelope protein which allows transduction of cells of human and other species, murine leukemia virus (MLV), Moloney murine leukemia virus (MoMLV), amphotropic MoMLV, Xenotropic MLV related retrovirus (XMRV), MLV related virus found in humans, xenotropic MLV, Polytropic MLV, amphotropic MLV, Gibbon ape leukemia virus (GALV), murine mammary tumor virus (MuMTV), Rous Sarcoma Virus (RSV), Gibbon ape leukemia virus (GALV), baboon endogenous virus (BEV), feline virus RD114, other gamma retrovirus, or hybrids of the above.
In addition, the disclosure provides polynucleotide sequence encoding a recombinant retroviral vector of the disclosure. The polynucleotide sequence can be incorporated into various viral particles. For example, various viral vectors which can be utilized for gene delivery include adenovirus, herpes virus, vaccinia, and baculovirus. Retroviruses can be prepared using such vectors (replicative or non-replicative) to deliver the retroviral genes to cells to make producer cells for the retrovirus. The retrovirus can then be harvested and purified according to the methods described in this specification. In yet another embodiment, the disclosure provides plasmids comprising a recombinant retroviral derived construct. The plasmid can be directly introduced into a target cell or a cell culture such as HT1080 or other tissue culture cells. The resulting cells release the retroviral vector into the culture medium.
The disclosure provides a polynucleotide construct comprising from 5′ to 3′: a promoter or regulatory region useful for initiating transcription; a psi packaging signal; a gag encoding nucleic acid sequence, a pol encoding nucleic acid sequence; an env encoding nucleic acid sequence. For example, the promoter can comprise a CMV promoter having a sequence as set forth in SEQ ID NO: 7, 8 or 9 from nucleotide 1 to about nucleotide 582 and may include modification to one or more (e.g., 2-5, 5-10, 10-20, 20-30, 30-50, 50-100 or more nucleic acid bases) so long as the modified promoter is capable of directing and initiating transcription. In one embodiment, the promoter or regulatory region comprises a CMV-R-U5 domain polynucleotide. The CMV-R-U5 domain comprises the immediately early promoter from human cytomegalovirus to the MLV R-U5 region. In one embodiment, the CMV-R-U5 domain polynucleotide comprises a sequence as set forth in SEQ ID NO: 7, 8 or 9 from about nucleotide 1 to about nucleotide 1202 or sequences that are at least 95% identical to a sequence as set forth in SEQ ID NO: 7, 8 or 9 wherein the polynucleotide promotes transcription of a nucleic acid molecule operably linked thereto. The gag domain of the polynucleotide may be derived from any number of retroviruses, but will typically be derived from murine leukemia virus (MLV), Moloney murine leukemia virus (MoMLV), amphotropic MoMLV, Xenotropic MLV related retrovirus (XMRV), MLV related virus found in humans, xenotropic MLV, Polytropic MLV, amphotropic MLV, Gibbon ape leukemia virus (GALV), murine mammary tumor virus (MuMTV), Rous Sarcoma Virus (RSV), Gibbon ape leukemia virus (GALV), baboon endogenous virus (BEV), feline virus RD114, other gamma retrovirus, or hybrids of the above.
In one embodiment the gag domain comprises a sequence from about nucleotide number 1203 to about nucleotide 2819 or a sequence having at least 60%, 70% 80%, 90%, 95%, 98%, 99% or 99.8% (rounded to the nearest 10th) identity thereto. The pol domain of the polynucleotide may be derived from any number of retroviruses, but will typically be derived from murine leukemia virus (MLV), Moloney murine leukemia virus (MoMLV), amphotropic MoMLV, Xenotropic MLV related retrovirus (XMRV), MLV related virus found in humans, xenotropic MLV, Polytropic MLV, amphotropic MLV, Gibbon ape leukemia virus (GALV), murine mammary tumor virus (MuMTV), Rous Sarcoma Virus (RSV), Gibbon ape leukemia virus (GALV), baboon endogenous virus (BEV), feline virus RD114, other gamma retrovirus, or hybrids of the above.
In one embodiment the pol domain comprises a sequence from about nucleotide number 2820 to about nucleotide 6358 or a sequence having at least 60%, 70% 80%, 90%, 95%, 98%, 99% or 99.9% (roundest to the nearest 10th) identity thereto. The env domain of the polynucleotide may be derived from any number of retroviruses, but will typically be derived from murine leukemia virus (MLV), Moloney murine leukemia virus (MoMLV), amphotropic MoMLV, Xenotropic MLV related retrovirus (XMRV), MLV related viruses found in humans, xenotropic MLV, Polytropic MLV, amphotropic MLV, Gibbon ape leukemia virus (GALV), murine mammary tumor virus (MuMTV), Rous Sarcoma Virus (RSV), Gibbon ape leukemia virus (GALV), baboon endogenous virus (BEV), feline virus RD114, other gamma retrovirus, or hybrids of the above.
In one embodiment, the env domain comprises a sequence from about nucleotide number 6359 to about nucleotide 8323 or a sequence having at least 60%, 70% 80%, 90%, 95%, 98%, 99% or 99.8% (roundest to the nearest 10th) identity thereto. The 3′ LTR can be derived from any number of retroviruses, typically murine leukemia virus (MLV), Moloney murine leukemia virus (MoMLV), amphotropic MoMLV, Xenotropic MLV related retrovirus (XMRV), MLV related virus found in humans, xenotropic MLV, Polytropic MLV, amphotropic MLV, Gibbon ape leukemia virus (GALV), murine mammary tumor virus (MuMTV), Rous Sarcoma Virus (RSV), Gibbon ape leukemia virus (GALV), baboon endogenous virus (BEV), feline virus RD114, other gamma retrovirus, or hybrids of the above.
In one embodiment, the 3′ LTR comprises a U3-R-U5 domain. In yet another embodiment, the LTR comprises a sequence as set forth in SEQ ID NO:7 or 9 from about nucleotide 9405 to about 9998 or a sequence that is at least 60%, 70% 80%, 90%, 95%, 98% or 99.5% (rounded to the nearest 10th) identical thereto.
The disclosure also provides a recombinant retroviral vector genome comprising from 5′ to 3′ a CMV-R-U5, fusion of the immediate early promoter from human cytomegalovirus to the MLV R-U5 region; a PBS, primer binding site for reverse transcriptase; a 5′ splice site; a ψ packaging signal; a gag, ORF for MLV group specific antigen; a pol, ORF for MLV polymerase polyprotein; a 3′ splice site; a 4070A env, ORF for envelope protein of MLV strain 4070A; a PPT, polypurine tract; and a U3-R-U5, MLV long terminal repeat. This structure is further depicted in
As described above, the disclosure provides a host cells (e.g., 293, 293T, HT1080 cells, PC3 cells, LNCaP cells, SupT1 cells, U87 cells, D17 cells, CF2 cells, HeLa, COS cell, Chinese Hamster Ovary (CHO)) that are transduced (transformed or transfected) with a virus or viral vector provided herein. The vector may be, for example, a plasmid (e.g., as used with 293T cells), a viral particle (as used with HT1080 cells), a phage, etc. The host cells can be cultured in conventional nutrient media modified as appropriate for activating promoters, selecting transformants, or amplifying a coding polynucleotide. Culture conditions, such as temperature, pH and the like, are those previously used with the host cell selected for expression, and will be apparent to those skilled in the art and in the references cited herein, including, e.g., Sambrook, Ausubel and Berger, as well as e.g., Freshney (1994) Culture of Animal Cells: A Manual of Basic Technique, 3rd ed. (Wiley-Liss, New York) and the references cited therein.
In one embodiment of the disclosure, a producer cell produces replication competent retroviral vectors having increased stability relative to retroviral vectors made by conventional transient transfection techniques. Such increased stability during production, infection and replication is important for the treatment of cell proliferative disorders. The combination of transduction efficiency, transgene stability and target selectivity is provided by the replication competent retrovirus. The compositions and methods provide insert stability and maintains transcription activity of the transgene and the translational viability of the encoded polypeptide.
Viral preparations of the current invention can be purified in several ways by centrifugation, ultrafitration, coprecipitation with ther agenst such as Polyethylene glycol (PEG) but optimally are highly purified by harvesting supernatants, filtering, treating with benzonase, and chromotography. Chromotograohy can be over various types of columns such sulphonated cellulose, OH-apatite or others, but is preferably on anion exchange columns (AEX) followed by size exclusion chromatography (SEC). These procedures allow concentration of the virus, followed by transition into the buffer in the SECF column. Preparations can then be ultrafiltered to sterilize and clarify the preparations before aliquotting for storage and use.
These viral preparations can be used to detect antibodies to viruses with some common epitopes, but viruses that are not necessarily identical to those used for detection. This is because of the related sequences and conformational epitopes in retroviruses in general (e.g., GB Mortuza et al. Nature 431:481-485 2004) and MLV related retroviruse in particular. The methods of the disclosure can use various protein and viral detection methods. In one embodiment, the methods comprise ELISA of whole virus bound on a plate, competitive binding assays, Western blots, and other methods known to those skilled in the art. These can be used on specialized commercial machines such as the Artitect System (Abbott) or in 96 well or 384 well microtiter formats for large sample number screening.
As used herein, a “biological sample” refers to a sample of tissue or fluid isolated from a subject, that commonly includes antibodies produced by the subject. Typical samples that include such antibodies are known in the art and include, but are not limited to, blood, plasma, serum, fecal matter, urine, bone marrow, bile, spinal fluid, lymph fluid, samples of the skin, secretions of the skin, respiratory, intestinal, and genitourinary tracts, tears, saliva, milk, blood cells, organs, biopsies and also samples of in vitro cell culture constituents including but not limited to conditioned media resulting from the growth of cells and tissues in culture medium, e.g., recombinant cells, and cell components. The fluids screened can be blood, plasma, serum, urine, saliva, bucchal swab, nasal swab, fine needle aspirate, semen, vaginal secretion, milk or other bodily fluid or extract, including feces.
A common solid support can be used in the assays and compositions of the disclosure. “Common solid support” intends a single solid matrix to which the retroviral particle used in the subject immunoassays are bound covalently or by noncovalent means such as hydrophobic adsorption.
“Immunologically reactive” means that an antigen will react specifically with anti-retroviral (e.g., anti-MLV) antibodies present in a biological sample from a subject or sample being tested.
“Immune complex” intends the combination formed when an antibody binds to an epitope on an antigen. Antibodies detected can be IgM, IgG, IgA, IgE or IgD, depending on the type of secondary antibody or other secondary binding reagent used in the assay. Detection methods for the secondary reagent can be horseradish peroxidase, alkaline phosphatase or any secondary detection system known to those skilled in the art.
As used herein, the terms “label” and “detectable label” refer to a molecule capable of detection, including, but not limited to, radioactive isotopes, fluorescers, chemiluminescers, chromophores, enzymes, enzyme substrates, enzyme cofactors, enzyme inhibitors, chromophores, dyes, metal ions, metal sols, ligands (e.g., biotin, avidin, strepavidin or haptens) and the like. The term “fluorescer” refers to a substance or a portion thereof which is capable of exhibiting fluorescence in the detectable range. Particular examples of labels which may be used under the invention include, but are not limited to, horse radish peroxidase (HRP), fluorescein, FITC, rhodamine, dansyl, umbelliferone, dimethyl acridinium ester (DMAE), Texas red, luminol, NADPH and α- or β-galactosidase.
In one embodiment, a viral particle produced by the methods of the disclosure is bound to a solid support. A sample from a subject is then contacted with the solid support under conditions wherein any antibodies to an epitope of the viral particle can for an immune complex with the viral particle. The solid support may be washed and then contacted with a secondary antibody (e.g., an anti-human antibody) labeled with a detectable label. The solid support can then be developed (as appropriate for the detectable label) and quantitated.
The following examples are meant to further illustrate the invention and are not meant to limit the broader disclosure above.
Replication of XMRV isolated from prostate cancer cells and CFS PBMCs has been reported and shows that the virus is capable of replicating in human cells. As noted above, XMRV has been suggested to be an artifact caused by contamination of samples by mouse DNA and recombination of laboratory Xenotropic isolates (T. Paprotka, et al., “Recombinant Origin of the Retrovirus XMRV” Science 333:97, 2011); J. Cohen & M. Enserink, “False Postive” Science 333:1694-1701, 2011; and G. Simmons et al., “Failure to Confirm XMRV/MLVs in the Blood of Patients with Chronic Fatigue Syndrome: A Multi-Laboratory Study” (www).sciencexpress.org/10.1126/science.1213841). However, as demonstrated here (see, e.g.,
22Rv1 (ATCC CRL-2505) is a human prostate cancer cell line derived from a primary prostatic carcinoma. Multiple integrated copies and high level production of XMRV was described by Knouf et al., J. virol 2009 op.cit). Quantitative PCR as described above revealed approximately 140 copy of XMRV in 22Rv1 genome with a titer of 1.5e7 TU/mL. This virus was used to determine whether a cell line will support XMRV infection. Similar methods can be used for other MLV related viruses.
22Rv1 cell culture supernatant containing XMRV titer of 1.5e7 TU/mL is used to infect a panel of cultured human cell lines: HT1080 (fibrosarcoma), U87-MG (glioma), LNCaP (prostate), and Sup-T1 (T-lymphoblasts). Each cell line is infected with XMRV at a MOI of 0.1. Viral replication kinetic in these cell lines are determined by qPCR using normalized gDNA from each passages and XMRV specific primer set lies in the env region (XMRV 6252F: 5′-TTT GAT TCC TCA GTG GGC TC-3′ (SEQ ID NO:1); XMRV6391R: 5′-CGA TAC AGT CTT AGT CCC CAT G-3′ (SEQ ID NO:2); XMRV env probe: 5′-HEX-CCC TTT TAC CCG CGT CAG TGA ATT CT-3′-BHQ (SEQ ID NO:3)), (
The viral vector is encoded by a plasmid (pAC3-yCD2; a.k.a. T5.0002, see, e.g., WO2010045002A2, the disclosure of which is incorporated herein by reference) consisting of 11,893 base pairs of nucleotides. The vector producer cell line “HT1080+T5.0002”, was produced by transducing naïve HT1080 cells with AC3-yCD2 virus produced transiently in 293 T cells by transfection. The transient transfection used to produce AC3-yCD2 (viral particles) was performed using a sequenced “qualified plasmid stock”. More specifically, transiently produced AC3-yCD2 vector was harvested after 48 hours post transfection and filtered through a 0.45 μm filter with 0.8 mL of filtered supernatant used to transduce a 75% confluent culture of HT1080 cells containing 15 mL of media. This infection volume converted to an approximate transduction dose of about 0.1 transduction unit (TU) per cell. The transduction was allowed to spread throughout the culture for 9 days with cells refed or passaged every 2-4 days prior to freezing down the initial Pre-Bank stock consisting of 12 vials with each vial containing approximately 5×106 cells per vial. The freezing media included 10% DMSO, USP (Cryoserv, Bioniche Pharma USA, LLC, Lake Forest, Ill.) and 90% gamma-irradiated fetal bovine serum (Hyclone Laboratories. Inc, Logan Utah). Cells were frozen in a −80° C. freezer and then transferred to a liquid nitrogen freezer under vapor phase conditions the following day. The media used for growing HT1080+T5.0002 cells to produce the vector comprises a defined DMEM media, GlutaMax (L-glutamine substitute), non-essential amino acids (NEAA), and Defined Fetal Bovine Serum (FBS).
The 293T cell line was developed from HEK (Human Embryonic Kidney) 293 cells and was originally described in 1987 (Dubridge 1987). The cell line was developed by transfecting a temperature sensitive SV40 T-antigen mutant, tsA1609 (Dubridge 1987), into HEK 293 cells (Graham 1977). The 293T cells are more susceptible to transfection than the original HEK 293 cell line. Because of their higher transfectability, 293T cells have commonly been used to produce high titer vectors by transient transfection (Yang et al., Hum. Gene Ther. 10:123-132, 1999).
HT1080+T5.0002 cells are grown in disposable multilayered cell culture vessels (Cell Stack, Corning). Production of the crude Toca 511 retroviral vector is performed by harvesting the conditioned media from confluent cultures of HT1080+T5.0002 cells harvested every 10-24 hour period over 2-4 harvest cycles using a manual fed batch process using multiple Cell Stacks containing approximately 1.2 L of conditioned media each. Crude Toca 511 retroviral vector is harvested directly into 10-20 L process bags and stored at 2-8° C. until approximately 40 L of material is collected. Sample of the combined crude pool harvest used for PTC mycloplasma, in vitro viral testing, bioburden, informational PCR titering and retentions.
The crude vector material is clarified by passing through a 0.45 micron filter cartridge and treated with Benzonase to digest host cell genomic DNA. The clarified and DNA digested Toca 511 vector is then captured and concentrated using anion exchange (AEX) chromatography. The eluted concentrated bulk product then undergoes a buffer exchange and purification step using size exclusion (SEC) chromatography. The formulation buffer comprises Tris-based formulation buffer containing sodium chloride, sucrose, mannitol, and human serum albumin. The formulated bulk is 0.2 micron filtered to insure sterility, sampled for testing and then divided in multiple containers as bulk material stored frozen below (<) −65° C.
The functional vector concentration, or titer, is determined using a quantitative PCR-based (qPCR) method. In this method, vector is titered by infecting a transducible host cell line (e.g. PC-3 human prostatic carcinoma cells, ATCC Cat#CRL-1435) with a standard volume of vector and measuring the resulting amount of provirus present within the host cells after transduction. The cells and vector are incubated under standard culturing condition (37° C., 5% CO2) for 24 hr to allow for complete infection prior to the addition of the anti-retroviral AZT to stop vector replication. Next, the cells are harvested from the culture dish and the genomic DNA (gDNA) is purified using an Invitrogen Purelink gDNA purification kit and eluted from the purification column with sterile RNase-/DNase-free water. The A260/A280 absorbance ratio is measured on a spectrophotometer to determine the concentration and relative purity of the sample. The gDNA concentrations are normalized with additional RNase-/DNase-free water to the lowest concentration of any given set of gDNA preparations such that the input DNA for the qPCR is constant for all samples analyzed. Genomic DNA purity is further assessed by electrophoresis of an aliquot of each sample on an ethidium bromide stained 0.8% agarose gel. If the sample passes an A260/A280 absorbance range of 1.8-2.0 and shows a single band of gDNA, then the sample is ready for qPCR analysis of provirus copy number of the vector. Using primers that interrogate the LTR region of the provirus (reverse-transcribed vector DNA and vector DNA that is integrated into the host gDNA), qPCR is performed to estimate the total number of transduction events that occurred when the known volume of vector was used to transduce the known number of cells. The number of transduction events per reaction is calculated from a standard curve that utilizes a target-carrying plasmid of known copy-number that is serial diluted from 107 to 10 copies and measured under identical qPCR conditions as the samples. Knowing how many genomic equivalents were used for each qPCR reaction (from the concentration previously determined) and how many transduction events that occurred per reaction, we determine the total number of transduction events that occurred based on the total number of cells that were present at the time of transduction. This value is the titer of the vector after dilution into the medium containing the cells during the initial transduction. To calculate the corrected titer value, the dilution is corrected for by multiplying through by the volume of culture and the volume of titer divided by the volume of titer. These experiments are performed in replicate cultures and analyzed by qPCR using triplicate measurements for each condition to determine an average titer and with its associated standard deviation and coefficient of variance.
Viruses of the disclosure are manufactured by commonly known transient transfection procedures of 293 or HT1080 cells, or from a producer cell non-clonal pool, or from a cloned producer cell line. Depending on cell line adaptation, the medium can be with serum or serum free independent, and the cells can be grown as adherent cells or in suspension, with cell culture supernatant collected under perfusion mode. The culture supernatant is harvested, and stored for up to 2 weeks at 4° C. This bulk harvest is filtered through a 0.45 micron filter cartridge to remove large cellular debri, treated with benzonase to digest cellular DNA (L. Shastry et al. Hum Gene Ther., 15:221, 2004) and subjected further to chromatography purification. (see, e.g., U.S. Pat. No. 5,792,643; T. Rodriguez et al., J Gene Med. 9:233, 2007; P. Sheridan et al., Mol. Ther., 2:262-275, 2000). The benzonase treated viral preparation is loaded on an anion exchange column and the virus is eluted in a stepwise NaCl gradient (see, e.g.,
Dilution Seeding.
Pre-warm media and Multiple 96 well cell culture plates were labelled in order to identify the clone based on the plate and well position and the wells were filled with prewarmed media containing single cell suspension of the HT1080. An early passage of 100% infected HT1080 cells was harvested by trypsinizing and creating a single cell suspension consisting of 1 cell per 600 microL. 200 μL was delivered into each well of a 96 well plate in order to seed approximately 0.3 cells per well. In performing this procedure, a majority of the wells received either 0, 1 or 2 cells per well. Cells were allowed to attach for approximately 4 hours and each well examined to eliminate wells that have received more than 1 cell per well or are empty.
Clone Propagation.
The wells that initially contained 1 cell per well were cultured by replacing 1/2 of the media (approximately 100 μL) with fresh 100 μL every 3-4 days for every well. Accidental transfer of cells from one well to another was avoided by replacing the tip used to feed each well during media replacement. Full media replacement was required as cells started to approach confluence in the well. Once the cells reached confluence, each clonal candidate was passaged to a well of a 48 well plate to continue expansion. Each clone was propagated and passed to a well of a 6 well plate, followed by a T-25 flask, followed by T-75 flask each time the cells reached confluence. Once the clonal cells reached confluence in a T-75 flask, at least 2-3 vials of cryopreserved cells containing 1-2×106 cells per vial were prepared.
Clone Selection Based on Performance.
Once the clone candidates were frozen down, cell culture experiments were performed to identify the best performing clone and back up clones based on titer production performance and ideal cell culture attributes. The best clone was chosen based on (1) the ability of the clone to provide the highest sustained titers over 2-4 subsequent days with daily media replacement (See
To produce D-17 (canine osteosarcoma; ATCC #CCL-183) and Cf2-Th (canine thymus; ATCC #CRL-1430) cell line vector producer pools and dilution clones that express MLV replication competent retrovirus, the exact same methods described above for HT-1080 cells were used to create D-17 and Cf2-Th cell lines. Results are shown in Table 2 below
The serum free adaptation process was performed after screening and identification of suitable dilution clone of HT-1080 replication competent virus producing cell line. The serum free adaptation process can also be performed with a non-clonal vector producing HT1080 cell line. The adaptation process was initiated by seeding approximately 2×107 cells into a 125 mL shaker flask containing 10 mL of 5% serum containing conditioned media and 10 mL of a selected serum free media of choice, resulting into a reduced serum concentration of 2.5%. In this case the serum free media was FreeStyle 293 Expression Media distributed through Invitrogen Corp, Carlsbad, Calif. The culture was placed on a shaking platform located in a tissue culture incubator with both temperature and CO2 gas control. The shaking platform was set to about 80 RPM and the incubator is set to about 37° C. and 5% CO2 conditions. Every 3-7 days, the culture was re-fed by collecting cells that are in suspension and reseeded into a new shaker flask containing 10 mL of the same initial conditioned media and 10 mL of fresh serum free media maintaining a level of serum of approximately 2.5%. The culture was examined at each re-feeding event with viable cell counts performed as needed to check for cell propagation. When the cells showed evidence of growth based on cell doubling or glucose consumption, a serum concentration of 1.67% was then targeted by adjusting the volume amount of condition media and fresh serum free media. The culture again was examined and refed every 3-7 days. When the cells show evidence of growth, a serum concentration of 1.25% was targeted by again adjusting the volume of conditioned media and fresh serum free media. This process was continued targeting subsequent serum conditions of 1.0%, 0.9%, 0.83% serum conditions until the cells were in 100% serum free conditions. During this adaptation process the cell culture was expanded to approximately 200 mL volume in a 1,000 mL shaking flask targeting a minimal viable culture of approximately 0.5 to 1.0×106 cell/mL. Once the cells reached 100% serum free conditions, the cells were continuously passaged under serum free conditions isolating single suspended cells by allowing heavier clumping cells to settle for short periods of time without agitation. Once the culture consists of approximately 95% population of the single cell suspension consistently, the culture could be frozen in cryopreservation media consisting of 10% DMSO and 90% serum free media using standard mammalian cell freezing conditions.
The example describes a cell culture technique for growing adherent cells producing murine leukemia virus (MLV) related viruses (MLVRV) for viral production and subsequent viral purification. Production of an amphotropic MLV virus (ATCC#VR-1450) was used as an example. However this process can also be used for all viruses described in this disclosure.
Wild type MuLV was obtained from ATCC but can also be generated from a plasmid construct (pAMS, ATCC #45167) encoding the entire MuLV genomic sequence with a genetically engineered amphotropic envelope gene replacing the original ecotropic envelope gene, and transiently transfected into cells using standard transfection techniques. Preferably, HT1080 cells (ATCC CCL-121) are infected with the virus and propagated under the preferred conditions of 37 C under 5% CO2 conditions. A newly infected T-75 flask containing MuLV infected HT1080 cells is expanded to two T-175's and then subsequently cultured to ten T-175 flasks with the following growth medium:
After reaching confluence, cells are harvested with TrpZean (Sigma) and neutralized with the same growth medium using standard cell culture methods. The cells are seeded into three 10-layer Cell Stacks (Corning) at a preferred seeding density of 3.1×10̂4 viable cells/cm2 in the same medium previously described above, to produce the virus. Each Cell Stack contained 1.1 L of the growth medium. The Cell Stacks were incubated at 37° C. and 5% CO2.
Two days after seeding, the Cell Stack cultures will reach confluence. The medium in each culture is replaced with fresh medium. Two days later, the medium, containing produced virus, is harvested (Harvest #1), and the cultures re-fed with the same volume (1.1 L) of fresh medium. Ten hours later, a second harvest (Harvest #2) is conducted and the cell cultures re-fed with same volume (1.1 L) of fresh growth medium. Sixteen hours following 2nd harvest, a 3rd harvest (Harvest #3) is performed. The 3 harvests are then pooled for purification. Viral titers for the 3 harvests and the pool are listed in the following table.
The same culturing technique described above are used to produce any of the disclosed viruses, in any of HT1080 cells, PC3 cells, LNCaPcells, SupT1 cells, U87 cells, D17 cells, CF2 cells, 293 cells, Hela cells, CV1 cells, CHO cells or cell lines derived from any of these. Three harvests, as described in Example 9, can be collected and pooled. The viral titer in the harvest pool using these cells is 105 TU/mL or greater, usually about 5×106 TU/mL.
A clonal or non clonal HT1080 viral producer cell line producing MLV related virus is adapted to zero-serum and suspension growth using a slow weaning process transitioning from 5% serum conditioned media to a defined serum free media. The adaptation process is initiated by seeding approximately 2×107 cells into a 125 mL shaker flask containing 10 mL of 5% serum containing conditioned media and 10 mL of a selected serum free media of choice, resulting in a reduced serum concentration of 2.5%. In this case the serum free media is FreeStyle 293 Expression Media distributed through Invitrogen Corp, Carlsbad, Calif., supplemented with 0.1% human serum albumin (with Baxter 25% human serum solution). The culture is placed on a shaking platform located in a tissue culture incubator with both temperature and CO2 gas control. The shaking platform is set to a about 80 RPM and the incubator is set to about 37° C. and 5% CO2 conditions. Every 3-7 days, the culture is re-fed by collecting cells that are in suspension and reseeded into a new shaker flask containing 10 mL of the same initial conditioned media and 10 mL of fresh serum free media maintaining a level of serum of approximately 2.5%. The culture is examined at each re-feeding event with cell counts taken as needed to check for cell propagation. When the cells show evidence of growth based on cell doubling or glucose consumption, a serum concentration of 1.67% is then targeted by adjusting the volume amount of condition media and fresh serum free media. The culture again is examined and refed every 3-7 days as before. When the cells show evidence of growth, a serum concentration of 1.25% is targeted by again adjusting the volume of conditioned media and fresh serum free media. This process is continued targeting subsequent serum conditions of 1.0%, 0.9%, 0.83% serum conditions until the cells are in 100% serum free conditions. During this adaptation process, the cell culture is expanded to approximately 200 mL volume in a 1,000 mL shaking flask targeting a minimal viable cell culture density of approximately 0.5 to 1.0×106 cell/mL. Once the cells reach 100% serum free conditions, the cells are continuously passaged under serum free conditions by isolating single suspended cells from the top half of the culture by allowing heavier clumping cells to settle for short periods of time without agitation. Once the culture consists of approximately 95% population of the single cell suspension consistently, the culture can be frozen in cryopreservation media consisting of 10% DMSO and 90% serum free media using standard mammalian cell freezing conditions.
The suspension cells are expanded in shake flasks—125-mL (20 mL culture), 250-mL (40 mL), 500-mL (100 mL), and 1-L (200 mL; all from Corning), in the fully defined serum-free medium (Gibco Cat#12338), supplemented with 0.1% human serum albumin (HSA, from Baxter). The cultures are incubated at 37° C. and 5% CO2 with shaker speed of 80 rpm (orbit ¾″). Five 1-L Shake Flask cultures are used to inoculate a WAVE bioreactor (WAVE 20/50 EHT, GE Healthcare) containing a 20-L Cellbag with 10 L working volume. The initial cell density in the bioreactor is a preferred 4×105 viable cells/mL (viability 91%). The temperature is a preferred 37° C. The initial operating conditions are: 5% CO2, rocker speed 15 rpm, angle 6°, air flow rate 0.2 L/min. The pH control is set at 7.2, and DO control at 40%. Both pH and DO controls are implemented by a WAVE POD console system (GE Healthcare).
After the cell density reaches about ˜1×106 viable cells/mL, a cell perfusion process is started using a hollow fiber cartridge (GE Healthcare, Part#CFP-6D-6A). The feed (and permeate) rate is initially set at 0.25 volume/day, and progressively increased with cell density for up to 3.8 volumes/day. A total of 180 L of permeate containing the virus is harvested within a 15 day period. The viral titer in the 180 L harvests is 1×105 TU/mL to 1×107 TU/mL.
A human anti-MLV IgG ELISA assay was developed for the detection of anti-MLV specific antibodies. The assay currently discriminates positive and negative samples and provides a titer for the antibody response. A monoclonal antibody, 83A25, specific for the gp70 protein of Moloney MLV was used as an assay positive control. 83A25 recognizes an epitope of gp70 env protein found universally in a variety of murine leukemia viruses including amphotropic leukemia virus (Evans et al., 1977, J. Virol., 24:865-874; Evans et al., 1990, J. Virol., 64(2):6176-6183). 83A25 is a rat IgG2a antibody. 83A25 mAb is derived from hybridoma cells (ATCC cat#HB-10392, Lot#100005) using standard antibody purification procedures. The assay is designed to detect the presence of anti-MLV antibodies in human samples. The assay positive control (83A25) is a rat antibody that is detected with an anti-rat Ig secondary. An anti-human IgG HRP conjugated antibodies is used for detection of human IgG.
The specific anti-MLV antibody capture ELISA was developed for antibody detection with species specific positive and negative controls. Briefly, 100 μL/well of capture antigen (1.56 μg/mL) was incubated overnight at 2-8° C. in ELISA 96-well microtiter plates (Costar). Plates were blocked with blocking buffer (1×PBS, 1% BSA, 0.05% TW20, 0.05% sodium azide). Human or canine sera collected at different time points across the studies was diluted 1:100 and was titred at 1:4 series in PBS added to the capture antigen coated plates and incubated for 60-90 min at room temperature with constant shaking at 150 RPM. Any bound antibody was detected with an HRP-conjugated goat anti-human IgG antibody or anti-canine IgG antibody (Southern Biotech). ELISAs were washed 5× with PBS and developed using a TMB substrate (Southern Biotech) for 10 min incubation and read on a SpectraMax 190 spectraphotometer at 450 nm. Positive controls were obtained from immunized animals, negative controls were obtained from healthy animals. Human donor panels were screened to identify suitable human positive and negative serum.
Human serum from 10 different healthy donors, tested negative for HBsAg, HIV ½ Ab, HIV-1 RNA, HCV Ab, HCV RNA, and STS and verbally screened to not have been in contact with any rodents (mice, rats, guinea pigs) including pets, as well as primates and to never have worked with any viruses, was purchased from BioReclamation Incorporation. All sera were screened using human anti-MLV ELISA protocol and serum tested negative for the presence of anti-amphotropic MLV antibodies were pooled and used as Negative Control Serum. Absorbance values (ODs) at 450 nm were generally recorded at below 0.400.
Reactivity of 83A25 is detected by an anti-rat Ig HRP conjugated antibody. As seen in
To evaluate the detection of a specific anti-MLV antibody within a serum matrix, the 83A25 antibody was spiked into human serum diluted 1:100 in dilution buffer or dilution buffer alone. The spikes were serial diluted and evaluated per the anti-MLV ELISA procedure.
Using plate bound MLV viral vector, detection of anti-MLV IgG antibodies in human sera or plasma is achieved using a direct assay. A competitive assay can be performed using the 83A25 antibody that recognizes gp70-env protein from amphotropic MLV virus. The titer of test samples is established by serially diluting 1:5 from a starting 1:100 dilution and compared to baseline samples; positive signals are defined as those above the cutoff established for that assay (greater than the mean negative control value+2 SD). Since 83A25 is a rat antibody, an assay threshold values based on the mean of the OD values+2SD of the dilution buffer is used.
Table 4 and Table 5 present inter-assay performance with replicate analysis of assay positive control and negative serum control.
Human plasma samples from individual donors were screened using the human anti-MLV ELISA assay. Samples that gave mean OD450 nm values below 0.200 were pooled and tested to generate a Negative Control (NC) plasma sample for use in future assays. By contrast, samples that gave high means OD450 nm values were selected as potential candidates for Positive Control (PC) plasma. These candidates were tested by Western blotting under non-reducing conditions to confirm the presence of anti-MLV specific antibodies. The sample that gave the best anti-MLV reactivity, and a high OD450 nm value as tested in ELISA, was selected as the Positive Control plasma for use in future assays.
Plasmas were obtained from Bioreclamation Inc. (Westbury, N.Y.) and collected in tubes containing K-2 EDTA as an anticoagulant. All samples tested negative for HBsAg, HIV 1 or 2 Ab, HIV-1 RNA, HCV Ab, hCV RNA and STS as reported by the supplier. Donors were classified as Black, Caucasian or Hispanic and varied from 20 to 78 years of age. Upon receipt the plasmas were stored at −80° C. until use. Plasmas from donors 866, 867, 871, 872 and 863 with mean OD450 nm values<0.200 (Table 6) were pooled to generate a Human Negative Control plasma (Lot#OT02DV-123-19Feb10). In the assays, the NC plasma is diluted 1:100 in Blocking/Dilution buffer with 8 well replicates per plate.
Plasma samples from donors that showed either high or intermediate mean OD450 nm values in the anti-MLV ELISA (Table 7) were selected to be analyzed further by Western blotting. This procedure would confirm anti-MLV specificity in the samples. Additionally, plasmas with high mean OD450 nm would serve as potential candidates for the Human Positive Control plasma for use in future assays. Further screening shows rare individuals (around 1/200) with ELISA readings in this assay of >3.0 OD units.
The example illustrates MLV viral antigen specificity of detected positive ELISA human samples. Plasma samples that showed high mean OD450 nm values in the anti-MLV ELISA were tested for antigen specificity in Western blotting. Selected plasmas were incubated with a PVDF membrane that contained MLV antigens that had been separated by electrophoresis under denaturing, non-reducing conditions. Lanes with HT1080 cell lysates and bovine serum albumin (BSA) were included as additional antigen samples. Antibodies in the plasma that recognized and bound to the different MLV antigens were then visualized using a horseradish peroxidase (HRP)-conjugated anti-human IgG antibody. Negative Control plasma (Lot#OT02DV-123-19Feb10) was used as a control in the assay.
Results of the Western blotting analysis are shown in
Two candidates were identified from the human plasma screen that gave the highest mean OD450 nm values as tested by ELISA (samples 131 and 194). To confirm the presence of antibodies to MLV in samples 131 and 194, Western blotting was performed using the same MLV antigen lot preparation used in the ELISA assay. Additional samples 856A and 183 that showed intermediate mean OD450 nm values were also included in the assay to compare the level of anti-MLV reactivity (
A human Positive Control plasma (PC) was generated from donor 131. The newly generated NC and PC plasma samples were with an example of PC titration curved presented in
This example illustrates direct detection of antibody against MLV in plasma from canines. Production of positive control serum for the anti-MLV IgG ELISA was achieved by challenging an experimental dog with the virus and collecting sera at specified time intervals. Ampho MLV (T5.0002) was used to immunize a beagle with adjuvant (1:1 dilution in complete freund's adjuvant), primed subcutaneously and at day 30, boosted w/vector (40 μg/dog) diluted 1:1 w/ Incomplete Freund's Adjuvant. Five subcutaneous injections (8 μg each) were given at day 15, 22 and 28 after priming; days 6 and 14 after boost (days 36 and 44 after priming).
A series of experiments were conducted to evaluate the anti-MLV response curves of sera from the immunized dog that had been collected at different timepoints following innoculation. The serum had been separated and stored at −80° C. until use. Sera collected on days 15, 22 and 28 gave weak, but progressively higher antibody response curves, culminating with a strong dose response on days 36 and 44. Similarity in the dose response curves of sera collected at these last two timepoints, and a comparison with d36+d44 pooled sera at a 1:5 dilution factor is shown in
The example illustrates the selectivity, sensitivity and reproducibility determined for the detection of MLV antibodies in canine samples.
Results generated with positive control serum (from dog immunized with viral vector) and negative control serum (from naïve dogs) suggests that signal in this assay is indeed a reflection of IgG binding to plate-bound viral vector. The titer of test samples will be established by serially diluting 1:4 from a starting 1:100 dilution and compared to pre-bleed samples; positive signals are defined as those above the cutoff established for that assay (greater than the mean negative control value+2 SD).
To assess inter-assay performance, data from several studies were analyzed, with replicate analysis of positive control and negative controls (Tables 8 and 9).
A four-parameter curve analysis for Positive Control sera was performed using data from several studies. The average of duplicates was used to generate the data in
The example illustrates direct detection of antibodies against MLV in sera of patients using direct assays. Two canine studies were conducted for evaluating immune responses to amphotropic MLV by intracranial and intravenous.
An immune response to MLV was observed in all the high dose groups E7 dogs (N=4) and E9 (N=2) (
The example illustrates the direct detection of amphotropic ENV viral protein in human samples by direct immunoblot approach.
An amphotropic MLV viral particle was serially diluted to give corresponding viral loads from 3.2e5 down to 1e4 and blotted onto a nitrocellulose membrane. Human plasma samples were diluted to 1:10, 1:20, 1:40 and 1:60 (final) and spiked with virus at 3.2e5, 1.6e5 and 8e4 (final). All samples were denatured/reduced in LDS buffer containing 2-ME and heated to 100° C. After blocking, the membrane was incubated with anti-gp70 antibody (clone: 83A25) at 1 μg/mL for 1 h at RT. The membranes were developed with a streptavidin:HRP system and visualized with a BioRad Imager (
The example illustrates the direct detection of XMRV proteins by immunoblotting using antibody clone 514.
Detection of viral gp70 expression in MLV and XMRV samples using the mouse monoclonal anti-gp70 antibody (clone 514) was tested by Western blotting. Purified virus TGFP6002-SEC and cell lysates from T5.0002-infected U87 cells were compared to cell lysates obtained from the 22Rv1 cell line. Samples were loaded on a 4-12% Bis-Tris gel (BioRad) at 1 μg/well total protein (TGFP6002-SEC) and 40 μg/well total proteins for the cell lysates. The gel was run at 200V for 35 min. The separated proteins were transferred to a PVDF membrane which was blocked overnight in 5% non-fat milk at 4° C. The membrane was then incubated with clone 514 (1 μg/mL) for 2 h at RT on a shaking plate. After washing with TBS-Tween, bound anti-gp70 was detected using a secondary HRP-labeled anti-mouse IgM antibody (1:2000, 1 h 45 min, RT) and a chemiluminescent substrate. A second anti-gp70 antibody (clone 83A25) was run as a control (
The example shows the detection of antibodies with the ELISA in high grade glioma (HGG) patients treated with Toca 511 by intracranial injection into the tumor followed by treatment with 5-fluorocytosine (5-FC). The trial is a multicenter, open-label, ascending-dose trial of the safety and tolerability of increasing doses of Toca 511 administered to subjects with recurrent HGG who have undergone surgery followed by adjuvant radiation and chemotherapy. The primary goal of the trial is to identify the highest, safe and well-tolerated dose of Toca 511 administered intratumorally via stereotactic, transcranial injection. Secondary goals are: to evaluate the safety and tolerability of treatment with 5-fluorocytosine (5-FC) at approximately 130 mg/kg/day for 6 days beginning approximately 3 weeks after administration of Toca 511 and repeated approximately every 4 weeks; to evaluate the objective response rate of the Toca 511/5-FC combination as assessed using the Macdonald criteria; to assess the percentage of subjects who have not progressed or died at 6 months (PFS-6); to assess the safety and tolerability of Toca 511 administered into residual tumor at the time of craniotomy and partial resection for recurrent high grade glioma (HGG) and followed by cyclic treatment with 5-FC.
Trial subjects are adults with recurrent HGG that has recurred following surgery, radiation and chemotherapy. Approximately three weeks after vector administration subjects underwent a baseline gadolinium-enhanced MRI (Gd-MRI) scan and then began treatment with oral 5-FC at approximately 130 mg/kg/day for 6 days. 6-day courses of 5-FC are repeated approximately every 4 weeks (±1 week) until institution of new antineoplastic treatment for tumor progression. Pts 101 and 102 had their tumors resected at the time frames shown in
This application claims priority under 35 U.S.C. §119 from Provisional Application Ser. No. 61/408,630, filed Oct. 31, 2010, the disclosure of which is incorporated herein by reference.
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/US2011/058457 | 10/28/2011 | WO | 00 | 3/3/2014 |
| Number | Date | Country | |
|---|---|---|---|
| 61408630 | Oct 2010 | US |
