Claims
- 1. A biologically pure culture of Antinomadura Q473-8 which produces the compound BMY-46164, which compound has an empirical formula of C.sub.40 H.sub.43 N.sub.2 O.sub.2 Cl and a molecular weight of 778, and the following spectral properties:
- (a) Mass Spectrum: FABMS: 778.2527, with prominent fragment ions at 294.1339 and 149.0603;
- (b) Ultraviolet Spectrum: A neutral 1.0 mg/100 ml methanol solution had absorption maximum .lambda..sub.max.sup.nm (E.sup.1%.sub.1cm):278 (635)
- (c) Infrared Spectrum: KBr pellet, cm.sup.-1 : 3424, 3076, 2934, 2838, 2170, 1640, 1578, 1520, 1490, 1462, 1424, 1376, 1292, 1248, 1184, 1156, 1112, 1072, 1046, 952, 900, 880, 832, 810, 754, 682, 666, 648, 576, 524;
- (d) 125 MHz .sup.13 C NMR: Bruker Model AM-500 Spectrometer. Proton decoupled spectrum. Dual carbon-proton probe, 5 mm. Solvent d.sub.6 DMSO. Observed chemical shifts (ppm): 168.4, 168.1, 153.3, 152.8, 139.3, 138.5, 133.0, 131.4, 131.3, 131.0, 130.8, 126.8, 124.7, 124.3, 122.5, 122.1, 121.0, 109.2, 97.5, 95.7, 94.3, 94.2, 93.3, 90.1, 74.0, 73.2, 70.9, 69.8, 69.6, 66.3, 62.5, 55.4, 54.4, 52.3, 43.2, 37.5, 33.6, 22.7, 19.5, 16.1; and
- (e) 500 MHz.sup.1 H-NMR: Bruker Model AM-500 Spectrometer. Dual carbon-proton probe, 5 mm. Solvent d.sub.6 -DMSO. Observed chemical shifts (ppm): 9.18 (br. 5, 1H), 8.09 (dd, 1H), 7.40 (d, 1H), 7.25 (s, 1H), 6.97 (d, 1H), 6.81 (d, 1H), 6.58 (d, 1H), 6.37 (t, 1H), 5.41 (s, 1H), 5.37 (dd, 1H), 5.27 (m, 1H), 5.13 (br.s, 1H), 4.91 (s, 1H), 4.89 (m, 1H), 4.65 (d, 1H), 4.22 (br.d, 1H), 4.13 (ddd, 1H), 3.94 (m, 1H), 3.80 (dd, 1H), 3.54 (s, 3H), 3.46 (dd, 1H), 3.21 (d, 1H), 3.12 (s, 3H), 2.94 (ddd, 1H), 2.73 (m, 1H), 2.71 (dd, 1H), 2.58 (dd, 1H), 2.31 (dd, 1H), 2.19 (s, 3H), 2.10 (s, 3H), 1.26 (s, 3H).
- 2. A biologically pure concentrate containing the compound BMY-46164 isolated from a fermentation product of Actinomadura strain Q473-8, which compound has an empirical formula of C.sub.40 H.sub.43 N.sub.2 O.sub.12 Cl and a molecular weight of 778, and the following spectral properties:
- (a) Mass Spectrum: FABMS: 778.2527, with prominent fragment ions at 294.1339 and 149.0603;
- (b) Ultraviolet Spectrum: A neutral 1.0 mg/100 ml methanol solution had absorption maximum .lambda..sub.max.sup.nm (E.sup.1%.sub.1cm):278 (635);
- (c) Infrared Spectrum: KBr pellet, cm.sup.-1 : 3424, 3076, 2934, 2838, 2170, 1640, 1578, 1520, 1490, 1462, 1424, 1376, 1292, 1248, 1184, 1156, 1112, 1072, 1046, 952, 900, 880, 832, 810, 754, 682, 666, 648, 576, 524; and
- (d) 125 MHz .sup.13 C NMR: Bruker Model AM-500 Spectrometer. Proton decoupled spectrum. Dual carbon-proton probe, 5 mm. Solvent d.sub.6 -DMSO. Observed chemical shifts (ppm: 168.4, 168.1 153.3, 152.8, 139.3, 138.5, 133.0, 131.4, 131.3, 131.0, 130.8, 126.8, 124.7, 124.3, 122.5, 122.1, 121.0, 109.2, 97.5, 95.7, 94.3, 94.2, 93.3, 90.1, 74.0, 73.2, 70.9, 69.8, 69.6, 66.3, 62.5, 55.4, 54.4, 52.3, 43.2, 37.5, 33.6, 22.7, 19.5, 16.1; and
- (e) 500 MHz.sup.1 H-HMR: Bruker Model AM-500 Spectrometer. Dual carbon-proton probe, 5 mm. solvent d.sub.6 -DMSO. Observed chemical shifts (ppm): 918 (br. 5,1H), 809 (dd, 1H), 7.40 (d, 1H), 7.25 (d, 1H), 6.97 (d, 1H), 6.81 (d, 1H), 6.58 (d, 1H), 6.37 (t, 1H), 5.41 (s, 1H), 5.37 (dd, 1H), 5.27 (m, 1H), 5.13 (br.s, 1H), 4.91 (s, 1H), 4.89 (m, 1H), 4.65 (d, 1H), 4.22 (br.d, 1H, 4.13 (ddd, 1H), 3.94 (m, 1H), 3.80 (dd, 1H), 3.54 (s, 3H), 3.46 (dd, 1H), 3.21 (d, 1H), 3.12 (s, 3H), 2.94 (ddd, 1H), 2.73 (m, 1H), 2.71 (dd, 1H), 2.58 (dd, 1H), 2.31 (dd, 1H), 2.19 (s, 3H), 2.10 (s, 3H), 1.26 (s, 3H).
- 3. A process for treating a patient suffering from a microbial infection comprising the step of administering to said patient an antibiotic effective amount of the compound BMY-46164 isolated from a fermentation product of Actinomadura strain Q473-8, which compound has an empirical formula of C.sub.40 H.sub.43 N.sub.2 O.sub.12 Cl and a molecular weight of 778, and has the following spectral properties:
- (a) Mass Spectrum: FABMS: 778.2527, with prominent fragment ions at 294.1339 and 149.0603;
- (b) Ultraviolet Spectrum: A neutral 1.0 mg/100 ml methanol solution had absorption maximum .lambda..sub.max.sup.nm (E.sup.1%.sub.1cm): 278 (635);
- (c) Infrared Spectrum: KBr pellet, cm.sup.-1 : 3424, 3076, 2934, 2838, 2170, 1640, 1578, 1520, 1490, 1462, 1424, 1376, 1292, 1248, 1184, 1156, 1112, 1072, 1 046, 952, 900, 880, 832, 810, 754, 682, 666, 648, 576, 524;
- (d) 125 MHz .sup.13 C NMR: Bruker Model AM-500 Spectrometer. Proton decoupled spectrum. Dual carbon-proton probe, 5mm. Solvent d.sub.6 -DMSO. Observed chemical shifts (ppm): 168.4, 168.1, 153.3, 152.8, 139.3, 138.5, 133.0, 131.4, 131.3, 131.0, 130.8, 126.8, 124.7, 124.3, 122.5, 122.1, 121.0, 109.2, 97.5, 95.7, 94.3, 94.2, 93.3, 90.1, 74.0, 73.2, 70.9, 69.8, 69.6, 66.3, 62.5, 55.4, 54.4, 52.3, 43.2, 37.5, 33.6, 22.7, 19.5, 16.1; and
- (e) 500 MHz.sup.1 H-NMR: Bruker Model AM-500 Spectrometer. Dual carbon-proton probe, 5 mm. Solvent d.sub.6 -DMSO. Observed chemical shifts (ppm): 9.18 (br.5, 1H, 8.09 (dd, 1H), 7.40 (d, 1H), 7.25 (d, 1H), 6.97 (d, 1H), 6.81 (d, 1H), 6.58 (d, 1H), 6.37 (t, 1H), 5.41 (s, 1H), 5.37 (dd, 1H), 5.27 (m 1H), 5.13 (br.s, 1H), 4.91 (s, 1H), 4.89 (m, 1H), 4.65 (d, 1H), 4.22 (br.d, 1H), 4.13 (ddd, 1H), 3.94 (m, 1H), 3.80 (dd, 1H), 3.54 (s, 3H), 3.46 (dd, 1H), 3.21 (d, 1H), 3.12 (s, 3H), 2.94 (ddd, 1H), 2.73 (m, 1H), 2.71 (dd, 1H), 2.58 (dd, 1H), 2.31 (dd, 1H), 2.19 (s, 3H), 2.10 (s, 3H), 1.26 (s, 3H).
CROSS-REFERENCE TO RELATED APPLICATIONS
This is a division of copending application U.S. Ser. No. 08/017,223, filed Feb. 12, 1993, now U.S. Pat. No. 5,281,417 which was a division of 07/780,516, filed Oct. 12, 1991 now U.S. Pat. No. 5,087,567.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5281417 |
Schroeder et al. |
Jan 1994 |
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Divisions (2)
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Number |
Date |
Country |
Parent |
17223 |
Feb 1993 |
|
Parent |
780516 |
Oct 1991 |
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