Patent Application
20160002180
The present invention provides compounds of Formula I useful as inhibitors of hepatitis C virus (HCV), as inhibitors of HCV infection, and for the prevention and treatment of hepatitis C infection.
Hepatitis C virus (HCV) infection is a major health problem that affects 170 million people worldwide and 3-4 million people in the United States (Armstrong, G. L., et al., Ann. Intern. Med. 2006, 144:705-714; Lauer, G. M., et al., N. Eng. J. Med. 2001, 345:41-52). HCV infection leads to chronic liver disease, such as cirrhosis and hepatocellular carcinoma in a substantial number of infected individuals. Chronic HCV infection associated liver cirrhosis and hepatocellular carcinoma are also the leading cause of liver transplantation in the United States. Current treatments for HCV infection include immunotherapy with pegylated interferon-α in combination with the nucleoside-analog ribavirin. Pegylated interferon-a in combination with ribavirin and one of the two recently approved HCV NS3 protease inhibitors Incivek or Victrelis is the current standard of care for the treatment of genotype 1 HCV infected patients, the most difficult to treat patient population. However, current HCV treatments are compromised by suboptimal sustained virological response rates and associated with severe side effects, as well as resistance to the protease inhibitors. Therefore there is a clear need for improved antiviral drugs with better efficacy, safety, and resistance profiles.
The infection of human hepatocytes by HCV, also known as HCV entry, is mediated by the functional interactions of virally-encoded envelope glycoproteins E1 and E2 and host cell co-receptors, followed by a receptor-mediated endocytosis processes. This HCV entry step is a putative target for therapeutic intervention. Several virally-encoded enzymes are also putative targets for therapeutic intervention, including a metalloprotease (NS2-3), a serine protease (NS3, amino acid residues 1-180), a helicase (NS3, full length), an NS3 protease cofactor (NS4A), a membrane protein (NS4B), a zinc metalloprotein (NSSA) and an RNA-dependent RNA polymerase (NS5B).
Systems have been developed to study the biology of HCV entry into host cells. Pseudotyping systems where the E1 and E2 glycoproteins are used to functionally replace the glycoproteins of retroviruses have been developed (Bartosch, B., Dubuisson, J. and Cosset, F.-L. J. Exp. Med. 2003, 197:633-642; Hsu, M. et al. Proc. Natl. Acad. Sci. USA. 2003, 100:7271-7276). These systems yield HCV pseudoparticles that bind to and enter host cells in a manner which is believed to be analogous to the natural virus, thus making them a convenient tool to study the viral entry steps as well as to identify inhibitors blocking this process.
There is a clear and long-felt need to develop effective therapeutics for treatment of HCV infection. Specifically, there is a need to develop compounds that selectively inhibit HCV viral entry and replication and that are useful for treating HCV-infected patients and protecting liver transplant patients from HCV re-infection. This application discloses novel compounds that are effective in prevention of HCV infection. Additionally, the disclosed compounds provide advantages for pharmaceutical uses, for example, with respect to their mechanism of action, binding, prevention of infection, inhibition efficacy, and target selectivity.
The application provides compound of formula I
wherein:
R1 is H, halo, lower alkyl, phenyl, lower alkoxy, lower alkyl sulfonyl, heterocycloalkyl, benzyl, amino, alkyl amino, dialkyl amino, or halo lower alkyl;
R2 and R3 are each independently H, halo, amino, or halo lower alkyl;
R4 and R5 are each independently absent, H or benzyl;
X is CX′ or N; and
X′ is H, halo, or cyano;
or a pharmaceutically acceptable salt thereof.
The application provides a method for preventing a Hepatitis C Virus (HCV) infection comprising administering to a patient in need thereof a therapeutically effective amount of a compound of Formula I.
The application provides a method for treating a Hepatitis C Virus (HCV) infection comprising administering to a patient in need thereof a therapeutically effective amount of a compound of Formula I.
The application provides a composition comprising a compound of Formula I and a pharmaceutically acceptable excipient.
Definitions
The phrase “a” or “an” entity as used herein refers to one or more of that entity; for example, a compound refers to one or more compounds or at least one compound. As such, the terms “a” (or “an”), “one or more”, and “at least one” can be used interchangeably herein.
As used in this specification, whether in a transitional phrase or in the body of the claim, the terms “comprise(s)” and “comprising” are to be interpreted as having an open-ended meaning.
That is, the terms are to be interpreted synonymously with the phrases “having at least” or “including at least”. When used in the context of a process, the term “comprising” means that the process includes at least the recited steps, but may include additional steps. When used in the context of a compound or composition, the term “comprising” means that the compound or composition includes at least the recited features or components, but may also include additional features or components.
As used herein, unless specifically indicated otherwise, the word “or” is used in the “inclusive” sense of “and/or” and not the “exclusive” sense of “either/or”.
The term “independently” is used herein to indicate that a variable is applied in any one instance without regard to the presence or absence of a variable having that same or a different definition within the same compound. Thus, in a compound in which R″ appears twice and is defined as “independently carbon or nitrogen”, both R″s can be carbon, both R″s can be nitrogen, or one R″ can be carbon and the other nitrogen.
When any variable occurs more than one time in any moiety or formula depicting and describing compounds employed or claimed in the present invention, its definition on each occurrence is independent of its definition at every other occurrence. Also, combinations of substituents and/or variables are permissible only if such compounds result in stable compounds.
The symbols “*” at the end of a bond or “ - - - ” drawn through a bond each refer to the point of attachment of a functional group or other chemical moiety to the rest of the molecule of which it is a part. Thus, for example:
A bond drawn into ring system (as opposed to connected at a distinct vertex) indicates that the bond may be attached to any of the suitable ring atoms.
The term “optional” or “optionally” as used herein means that a subsequently described event or circumstance may, but need not, occur, and that the description includes instances where the event or circumstance occurs and instances in which it does not. For example, “optionally substituted” means that the optionally substituted moiety may incorporate a hydrogen atom or a substituent.
If a substituent is designated to be “absent”, the substituent is not present.
The term “about” is used herein to mean approximately, in the region of, roughly, or around. When the term “about” is used in conjunction with a numerical range, it modifies that range by extending the boundaries above and below the numerical values set forth. In general, the term “about” is used herein to modify a numerical value above and below the stated value by a variance of 20%.
Certain compounds may exhibit tautomerism. Tautomeric compounds can exist as two or more interconvertable species. Prototropic tautomers result from the migration of a covalently bonded hydrogen atom between two atoms. Tautomers generally exist in equilibrium and attempts to isolate an individual tautomers usually produce a mixture whose chemical and physical properties are consistent with a mixture of compounds. The position of the equilibrium is dependent on chemical features within the molecule. For example, in many aliphatic aldehydes and ketones, such as acetaldehyde, the keto form predominates while; in phenols, the enol form predominates. Common prototropic tautomers include keto/enol (—C(═O)—CH— —C(—OH)═CH—), amide/imidic acid (—C(═O)—NH—
—C(—OH)═N—) and amidine (—C(═NR)—NH—
—C(—NHR)═N—) tautomers. The latter two are particularly common in heteroaryl and heterocyclic rings and the present invention encompasses all tautomeric forms of the compounds.
Technical and scientific terms used herein have the meaning commonly understood by one of skill in the art to which the present invention pertains, unless otherwise defined. Reference is made herein to various methodologies and materials known to those of skill in the art. Standard reference works setting forth the general principles of pharmacology include Goodman and Gilman's The Pharmacological Basis of Therapeutics, 10th Ed., McGraw Hill Companies Inc.,
New York (2001). Any suitable materials and/or methods known to those of skill can be utilized in carrying out the present invention. However, preferred materials and methods are described. Materials, reagents and the like to which reference are made in the following description and examples are obtainable from commercial sources, unless otherwise noted.
The definitions described herein may be appended to form chemically-relevant combinations, such as “heteroalkylaryl,” “haloalkylheteroaryl,” “arylalkylheterocyclyl,” “alkylcarbonyl,” “alkoxyalkyl,” and the like. When the term “alkyl” is used as a suffix following another term, as in “phenylalkyl,” or “hydroxyalkyl,” this is intended to refer to an alkyl group, as defined above, being substituted with one to two substituents selected from the other specifically-named group. Thus, for example, “phenylalkyl” refers to an alkyl group having one to two phenyl substituents, and thus includes benzyl, phenylethyl, and biphenyl. An “alkylaminoalkyl” is an alkyl group having one to two alkylamino substituents. “Hydroxyalkyl” includes 2-hydroxyethyl, 2-hydroxypropyl, 1-(hydroxymethyl)-2-methylpropyl, 2-hydroxybutyl, 2,3-dihydroxybutyl, 2-(hydroxymethyl), 3-hydroxypropyl, and so forth. Accordingly, as used herein, the term “hydroxyalkyl” is used to define a subset of heteroalkyl groups defined below. The term -(ar)alkyl refers to either an unsubstituted alkyl or an aralkyl group. The term (hetero)aryl or (het)aryl refers to either an aryl or a heteroaryl group.
The term “carbonyl” or “acyl” as used herein denotes a group of formula —C(═O)R wherein R is hydrogen or lower alkyl as defined herein.
The term “ester” as used herein denotes a group of formula —C(═O)OR wherein R is lower alkyl as defined herein.
The term “alkyl” as used herein denotes an unbranched or branched chain, saturated, monovalent hydrocarbon residue containing 1 to 10 carbon atoms. The term “lower alkyl” denotes a straight or branched chain hydrocarbon residue containing 1 to 6 carbon atoms. “C1-10 alkyl” as used herein refers to an alkyl composed of 1 to 10 carbons. Examples of alkyl groups include, but are not limited to, lower alkyl groups include methyl, ethyl, propyl, i-propyl, n-butyl, i-butyl, t-butyl or pentyl, isopentyl, neopentyl, hexyl, heptyl, and octyl.
When the term “alkyl” is used as a suffix following another term, as in “phenylalkyl,” or “hydroxyalkyl,” this is intended to refer to an alkyl group, as defined above, being substituted with one to two substituents selected from the other specifically-named group. Thus, for example, “phenylalkyl” denotes the radical R′R″—, wherein R′ is a phenyl radical, and R″ is an alkylene radical as defined herein with the understanding that the attachment point of the phenylalkyl moiety will be on the alkylene radical. Examples of arylalkyl radicals include, but are not limited to, benzyl, phenylethyl, 3-phenylpropyl. The terms “arylalkyl” or “aralkyl” are interpreted similarly except R′ is an aryl radical. The terms “(het)arylalkyl” or “(het)aralkyl” are interpreted similarly except R′ is optionally an aryl or a heteroaryl radical.
The terms “haloalkyl” or “halo lower alkyl” or “lower haloalkyl” refers to a straight or branched chain hydrocarbon residue containing 1 to 6 carbon atoms wherein one or more carbon atoms are substituted with one or more halogen atoms.
The term “alkylene” or “alkylenyl” as used herein denotes a divalent saturated linear hydrocarbon radical of 1 to 10 carbon atoms (e.g., (CH2)n) or a branched saturated divalent hydrocarbon radical of 2 to 10 carbon atoms (e.g., —CHMe- or —CH2CH(i-Pr)CH2—), unless otherwise indicated. Except in the case of methylene, the open valences of an alkylene group are not attached to the same atom. Examples of alkylene radicals include, but are not limited to, methylene, ethylene, propylene, 2-methyl-propylene, 1,1-dimethyl-ethylene, butylene, 2-ethylbutylene.
The term “alkoxy” as used herein means an —O-alkyl group, wherein alkyl is as defined above such as methoxy, ethoxy, n-propyloxy, i-propyloxy, n-butyloxy, i-butyloxy, t-butyloxy, pentyloxy, hexyloxy, including their isomers. “Lower alkoxy” as used herein denotes an alkoxy group with a “lower alkyl” group as previously defined. “C1-10 alkoxy” as used herein refers to an-O-alkyl wherein alkyl is C1-10.
The terms “haloalkoxy” or “halo lower alkoxy” or “lower haloalkoxy” refers to a lower alkoxy group, wherein one or more carbon atoms are substituted with one or more halogen atoms.
The term “hydroxyalkyl” as used herein denotes an alkyl radical as herein defined wherein one to three hydrogen atoms on different carbon atoms is/are replaced by hydroxyl groups. The term “sulfinyl” as used herein denotes a —SO— group.
The term “sulfonyl” as used herein denotes a —SO2— group.
The terms “alkylsulfonyl” and “arylsulfonyl” as used herein refers to a group of formula —S(═O)2R wherein R is alkyl or aryl respectively and alkyl and aryl are as defined herein. The term “heteroalkylsulfonyl” as used herein refers herein denotes a group of formula —S(═O)2R wherein R is “heteroalkyl” as defined herein.
The term “lower alkyl sulfonylamido” as used herein refers to a group of formula —S(═O)2NR2 wherein each R is independently hydrogen or C1-3 alkyl, and lower alkyl is as defined herein.
The term “trifluoromethyl sulfonyl” as used herein refers to a group of formula —S(═O)2CF3.
The term “trifluoromethyl sulfinyl” as used herein refers to a group of formula —S(═O)CF3.
The term “trifluoromethyl sulfanyl” as used herein refers to a group of formula —SCF3.
The term “nitro” as used herein refers to a group of formula —N+(═O)O−.
The term “carboxyl” as used herein refers to a group of formula —C(═O)R2 wherein each R is independently hydrogen or C1-3 alkyl, and lower alkyl is as defined herein.
The term “cycloalkyl” denotes a monovalent saturated monocyclic or bicyclic hydrocarbon group of 3 to 10 ring carbon atoms. In particular embodiments cycloalkyl denotes a monovalent saturated monocyclic hydrocarbon group of 3 to 8 ring carbon atoms. Bicyclic means consisting of two saturated carbocycles having one or more carbon atoms in common. Particular cycloalkyl groups are monocyclic. Examples for monocyclic cycloalkyl are cyclopropyl, cyclobutanyl, cyclopentyl, cyclohexyl or cycloheptyl. Examples for bicyclic cycloalkyl are bicyclo[2.2.1]heptanyl, or bicyclo[2.2.2]octanyl.
The term “amino” as used herein denotes a group of the formula —NR′R″ wherein R′ and R″ are independently hydrogen, alkyl, alkoxy, cycloalkyl, heterocycloalkyl, aryl or heteroaryl. Alternatively, R′ and R″, together with the nitrogen to which they are attached, can form a heterocycloalkyl. The term “primary amino” denotes a group wherein both R′ and R″ are hydrogen. The term “secondary amino” denotes a group wherein R′ is hydrogen and R″ is not. The term “tertiary amino” denotes a group wherein both R′ and R″ are not hydrogen. Particular secondary and tertiary amines are methylamine, ethylamine, propylamine, isopropylamine, phenylamine, benzylamine dimethylamine, diethylamine, dipropylamine and diisopropylamine.
The term “amido” as used herein denotes a group of the formula —C(═O)NR′R″ or —NR′C(′O)R″ wherein R′ and R″ are independently hydrogen, alkyl, alkoxy, cycloalkyl, heterocycloalkyl, aryl or heteroaryl.
The term “heteroaryl” denotes a monovalent aromatic heterocyclic mono- or bicyclic ring system of 5 to 12 ring atoms, comprising 1, 2, 3 or 4 heteroatoms selected from N, O and S, the remaining ring atoms being carbon. Examples of heteroaryl moieties include pyrrolyl, furanyl, thienyl, imidazolyl, oxazolyl, thiazolyl, triazolyl, oxadiazolyl, thiadiazolyl, tetrazolyl, pyridinyl, pyrazinyl, pyrazolyl, pyridazinyl, pyrimidinyl, triazinyl, azepinyl, diazepinyl, isoxazolyl, benzofuranyl, isothiazolyl, benzothienyl, indolyl, isoindolyl, isobenzofuranyl, benzimidazolyl, benzoxazolyl, benzoisoxazolyl, benzothiazolyl, benzoisothiazolyl, benzooxadiazolyl, benzothiadiazolyl, benzotriazolyl, purinyl, quinolinyl, isoquinolinyl, quinazolinyl, or quinoxalinyl.
The term “heterocycloalkyl” denotes a monovalent saturated or partly unsaturated mono- or bicyclic ring system of 3 to 9 ring atoms, comprising 1, 2, or 3 ring heteroatoms selected from N, O and S, the remaining ring atoms being carbon. In particular embodiments, heterocycloalkyl is a monovalent saturated monocyclic ring system of 4 to 7 ring atoms, comprising 1, 2, or 3 ring heteroatoms selected from N, O and S, the remaining ring atoms being carbon. Examples for monocyclic saturated heterocycloalkyl are aziridinyl, oxiranyl, azetidinyl, oxetanyl, pyrrolidinyl, tetrahydrofuranyl, tetrahydro-thienyl, pyrazolidinyl, imidazolidinyl, oxazolidinyl, isoxazolidinyl, thiazolidinyl, piperidinyl, tetrahydropyranyl, tetrahydrothiopyranyl, piperazinyl, morpholinyl, thiomorpholinyl, 1,1-dioxo-thiomorpholin-4-yl, azepanyl, diazepanyl, homopiperazinyl, or oxazepanyl. Examples for bicyclic saturated heterocycloalkyl are 8-aza-bicyclo[3.2.1]octyl, quinuclidinyl, 8-oxa-3-aza-bicyclo[3.2.11octyl, 9-aza-bicyclo[3.3.11nonyl, 3-oxa-9-aza-bicyclo[3.3.1]nonyl, or 3-thia-9-aza-bicyclo[3.3.1]nonyl. Examples for partly unsaturated heterocycloalkyl are dihydrofuryl, imidazolinyl, dihydro-oxazolyl, tetrahydro-pyridinyl, or dihydropyranyl.
Inhibitors of HCV Entry
The application provides a compound of formula I
wherein:
R1 is H, halo, lower alkyl, phenyl, lower alkoxy, lower alkyl sulfonyl, heterocycloalkyl, benzyl, amino, alkyl amino, dialkyl amino, or halo lower alkyl;
R2 and R3 are each independently H, halo, amino, or halo lower alkyl;
R4 and R5 are each independently absent, H or benzyl;
X is CX′ or N; and
X′ is H, halo, or cyano;
or a pharmaceutically acceptable salt thereof.
The application provides a compound of formula I, wherein R4 is absent.
The application provides the above compound of formula I, wherein R5 is H.
The application provides the above compound of formula I, wherein R2 and R3 are Cl.
The application provides any of the above compounds of formula I, wherein X is N.
The application alternatively provides any of the above compounds of formula I, wherein X is CX′ and X′ is H.
The application provides any of the above compounds of formula I, wherein X is CX′ and X′ is halo or cyano.
The application provides any of the above compounds of formula I, wherein R1 is H, halo, lower alkyl, phenyl, lower alkoxy, lower alkyl sulfonyl, heterocycloalkyl, benzyl, amino, alkyl amino, dialkyl amino, or halo lower alkyl
The application provides a compound of formula I, wherein R5 is benzyl.
The application provides a compound of formula I, wherein R4 is absent R5 is benzyl.
The application provides the above compound of formula I, wherein R2 is amino or Cl, R3 is H or Cl, and X is CX′ and X′ is H or F.
The application provides a compound of formula I, wherein R4 is benzyl.
The application provides a compound of formula I, wherein R5 is absent and R4 is benzyl.
The application provides the above compound of formula I, wherein R2 is amino or Cl, R3 is H or Cl, and X is CX′ and X′ is H or F.
The application provides a compound selected from the group consisting of:
(3,5-Dichloro-phenyl)-(5-methyl-1H-[1,2,4]triazol-3-yl)-amine;
(3,5-Dichloro-phenyl)-(5-phenyl-1H-[1,2,4]triazol-3-yl)-amine;
(5-Aminomethyl-1H-[1,2,4]triazol-3-yl)-(3,5-dichloro-phenyl)-amine;
(3,5-Dichloro-phenyl)-(5-fluoro-2H-[1,2,4]triazol-3-yl)-amine;
(3,5-Dichloro-phenyl)-(5-methoxymethyl-2H-[1,2,4]triazol-3-yl)-amine;
(2,6-Dichloro-pyridin-4-yl)-(5-methoxymethyl-2H-[1,2,4]triazol-3-yl)-amine;
(5-Bromo-2H-[1,2,4]triazol-3-yl)-(3,5-dichloro-phenyl)-amine;
(3,5-Dichloro-phenyl)-(5-methoxy-2H-[1,2,4]triazol-3-yl)-amine;
(2,6-Dichloro-pyridin-4-yl)-(5-methoxy-2H-[1,2,4]triazol-3-yl)-amine;
4-(5-Methoxy-2H-[1,2,4]triazol-3-ylamino)-2-trifluoromethyl-benzonitrile;
(4-Bromo-3,5-dichloro-phenyl)-(5-methoxy-2H-[1,2,4]triazol-3-yl)-amine;
(5-Chloro-2H-[1,2,4]triazol-3-yl)-(3,5-dichloro-phenyl)-amine;
(3,5-Dichloro-phenyl)-(5-methanesulfonyl-2H-[1,2,4]triazol-3-yl)-amine;
(4-Bromo-3,5-dichloro-phenyl)-(5-methanesulfonyl-2H-[1,2,4]triazol-3-yl)-amine
N3-(3,5-Dichloro-phenyl)-N5,N5-dimethyl-1H-[1,2,4]triazole-3,5-diamine
N3-(4-Bromo-3,5-dichloro-phenyl)-N5,N5-dimethyl-1H-[1,2,4]triazole-3,5-diamine;
(2H-[1,2,4]Triazol-3-yl)-(3,4,5-trichloro-phenyl)-amine;
(4-Bromo-3,5-dichloro-phenyl)-(2H-[1,2,4]triazol-3-yl)-amine;
N3-(3,5-Dichloro-phenyl)-N5-methyl-1H-[1,2,4]triazole-3,5-diamine;
2,6-Dichloro-4-(2H-[1,2,4]triazol-3-ylamino)-benzonitrile;
4-(5-Methylamino-1H-[1,2,4]triazol-3-ylamino)-2-trifluoromethyl-benzonitrile;
(3,5-Dichloro-phenyl)-(5-trifluoromethyl-1H-[1,2,4]triazol-3-yl)-amine;
(1-Benzyl-1H-[1,2,4]triazol-3-yl)-(3,5-dichloro-phenyl)-amine;
(2-Benzyl-2H-[1,2,4]triazol-3-yl)-(3,5-dichloro-phenyl)-amine;
(3,5-Dichloro-phenyl)-(1H-[1,2,4]triazol-3-yl)-amine;
(2-Benzyl-2H-[1,2,4]triazol-3-yl)-(3,5-dichloro-4-fluoro-phenyl)-amine;
(1-Benzyl-1H-[1,2,4]triazol-3-yl)-(3,5-dichloro-4-fluoro-phenyl)-amine;
(3,5-Dichloro-4-fluoro-phenyl)-(1H-[1,2,4]triazol-3-yl)-amine;
N-(1-Benzyl-1H-[1,2,4]triazol-3-yl)-benzene-1,3-diamine;
(2,6-Dichloro-pyridin-4-yl)-(1H-[1,2,4]triazol-3-yl)-amine;
(3,5-Dichloro-4-fluoro-phenyl)-(5-morpholin-4-yl-1H-[1,2,4]triazol-3-yl)-amine;
(3,5-Dichloro-phenyl)-(5-isopropyl-1H-[1,2,4]triazol-3-yl)-amine;
(3,5-Dichloro-phenyl)-(5-isobutyl-1H-[1,2,4]triazol-3-yl)-amine; and
(5-Benzyl-1H-[1,2,4]triazol-3-yl)-(3,5-dichloro-phenyl)-amine.
The application provides a method for preventing a Hepatitis C Virus (HCV) infection comprising administering to a patient in need thereof a therapeutically effective amount of a compound of Formula I.
The application provides the above method, further comprising administering to a patient in need thereof a therapeutically effective amount of an immune system suppressant.
The application provides a method for treating a Hepatitis C Virus (HCV) infection comprising administering to a patient in need thereof a therapeutically effective amount of a compound of Formula I.
The application provides any of the above methods, further comprising administering a combination of antiviral agents that inhibits replication of HCV.
The application provides any of the above methods, further comprising administering an immune system modulator or an antiviral agent that inhibits replication of HCV, or a combination thereof.
The application provides the above method, wherein the immune system modulator is an interferon or a chemically derivatized interferon.
The application provides any of the above methods, further comprising administering an immune system modulator or an antiviral agent that inhibits replication of HCV, or a combination thereof, wherein the antiviral agent is selected from the group consisting of a HCV protease inhibitor, a HCV polymerase inhibitor, a HCV helicase inhibitor, a HCV NS5A inhibitor, or any combination thereof.
The application provides a composition comprising a compound of Formula I and a pharmaceutically acceptable excipient.
The application provides the use of the compound of Formula I in the preparation of a medicament for the prevention of HCV.
The application provides the use of the compound of Formula I in the preparation of a medicament for the treatment of HCV.
The application provides any compound, composition, method or use as described herein.
Compounds
Examples of representative compounds encompassed by the present invention and within the scope of the invention are provided in the following Table. These examples and preparations which follow are provided to enable those skilled in the art to more clearly understand and to practice the present invention. They should not be considered as limiting the scope of the invention, but merely as being illustrative and representative thereof.
In general, the nomenclature used in this Application is based on AUTONOMTM v.4.0, a Beilstein Institute computerized system for the generation of IUPAC systematic nomenclature. If there is a discrepancy between a depicted structure and a name given that structure, the depicted structure is to be accorded more weight. In addition, if the stereochemistry of a structure or a portion of a structure is not indicated with, for example, bold or dashed lines, the structure or portion of the structure is to be interpreted as encompassing all stereoisomers of it.
TABLE I depicts examples of compounds according to generic Formula I:
Synthesis
General Schemes
The following schemes depict general methods for obtaining compounds of Formula I.
Dosage and Administration:
The compounds of the present invention may be formulated in a wide variety of oral administration dosage forms and carriers. Oral administration can be in the form of tablets, coated tablets, dragées, hard and soft gelatin capsules, solutions, emulsions, syrups, or suspensions. Compounds of the present invention are efficacious when administered by other routes of administration including continuous (intravenous drip) topical parenteral, intramuscular, intravenous, subcutaneous, transdermal (which may include a penetration enhancement agent), buccal, nasal, inhalation and suppository administration, among other routes of administration. The preferred manner of administration is generally oral using a convenient daily dosing regimen which can be adjusted according to the degree of affliction and the patient's response to the active ingredient.
A compound or compounds of the present invention, as well as their pharmaceutically useable salts, together with one or more conventional excipients, carriers, or diluents, may be placed into the form of pharmaceutical compositions and unit dosages. The pharmaceutical compositions and unit dosage forms may be comprised of conventional ingredients in conventional proportions, with or without additional active compounds or principles, and the unit dosage forms may contain any suitable effective amount of the active ingredient commensurate with the intended daily dosage range to be employed. The pharmaceutical compositions may be employed as solids, such as tablets or filled capsules, semisolids, powders, sustained release formulations, or liquids such as solutions, suspensions, emulsions, elixirs, or filled capsules for oral use; or in the form of suppositories for rectal or vaginal administration; or in the form of sterile injectable solutions for parenteral use. A typical preparation will contain from about 5% to about 95% active compound or compounds (w/w). The term “preparation” or “dosage form” is intended to include both solid and liquid formulations of the active compound and one skilled in the art will appreciate that an active ingredient can exist in different preparations depending on the target organ or tissue and on the desired dose and pharmacokinetic parameters.
The term “excipient” as used herein refers to a compound that is useful in preparing a pharmaceutical composition, generally safe, non-toxic and neither biologically nor otherwise undesirable, and includes excipients that are acceptable for veterinary use as well as human pharmaceutical use. The compounds of this invention can be administered alone but will generally be administered in admixture with one or more suitable pharmaceutical excipients, diluents or carriers selected with regard to the intended route of administration and standard pharmaceutical practice.
“Pharmaceutically acceptable” means that which is useful in preparing a pharmaceutical composition that is generally safe, non-toxic, and neither biologically nor otherwise undesirable and includes that which is acceptable for veterinary as well as human pharmaceutical use.
A “pharmaceutically acceptable salt” form of an active ingredient may also initially confer a desirable pharmacokinetic property on the active ingredient which were absent in the non-salt form, and may even positively affect the pharmacodynamics of the active ingredient with respect to its therapeutic activity in the body. The phrase “pharmaceutically acceptable salt” of a compound means a salt that is pharmaceutically acceptable and that possesses the desired pharmacological activity of the parent compound. Such salts include: (1) acid addition salts, formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like; or formed with organic acids such as acetic acid, propionic acid, hexanoic acid, cyclopentanepropionic acid, glycolic acid, pyruvic acid, lactic acid, malonic acid, succinic acid, malic acid, maleic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, 3-(4-hydroxybenzoyl)benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, 1,2-ethane-disulfonic acid, 2-hydroxyethanesulfonic acid, benzenesulfonic acid, 4-chlorobenzenesulfonic acid, 2-naphthalenesulfonic acid, 4-toluenesulfonic acid, camphorsulfonic acid, 4-methylbicyclo[2.2.2]-oct-2-ene-l-carboxylic acid, glucoheptonic acid, 3-phenylpropionic acid, trimethylacetic acid, tertiary butylacetic acid, lauryl sulfuric acid, gluconic acid, glutamic acid, hydroxynaphthoic acid, salicylic acid, stearic acid, muconic acid, and the like; or (2) salts formed when an acidic proton present in the parent compound either is replaced by a metal ion, e.g., an alkali metal ion, an alkaline earth ion, or an aluminum ion; or coordinates with an organic base such as ethanolamine, diethanolamine, triethanolamine, tromethamine, N-methylglucamine, and the like.
Solid form preparations include powders, tablets, pills, capsules, cachets, suppositories, and dispersible granules. A solid carrier may be one or more substances which may also act as diluents, flavoring agents, solubilizers, lubricants, suspending agents, binders, preservatives, tablet disintegrating agents, or an encapsulating material. In powders, the carrier generally is a finely divided solid which is a mixture with the finely divided active component. In tablets, the active component generally is mixed with the carrier having the necessary binding capacity in suitable proportions and compacted in the shape and size desired. Suitable carriers include but are not limited to magnesium carbonate, magnesium stearate, talc, sugar, lactose, pectin, dextrin, starch, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose, a low melting wax, cocoa butter, and the like. Solid form preparations may contain, in addition to the active component, colorants, flavors, stabilizers, buffers, artificial and natural sweeteners, dispersants, thickeners, solubilizing agents, and the like.
Liquid formulations also are suitable for oral administration include liquid formulation including emulsions, syrups, elixirs, aqueous solutions, aqueous suspensions. These include solid form preparations which are intended to be converted to liquid form preparations shortly before use. Emulsions may be prepared in solutions, for example, in aqueous propylene glycol solutions or may contain emulsifying agents such as lecithin, sorbitan monooleate, or acacia. Aqueous solutions can be prepared by dissolving the active component in water and adding suitable colorants, flavors, stabilizing, and thickening agents. Aqueous suspensions can be prepared by dispersing the finely divided active component in water with viscous material, such as natural or synthetic gums, resins, methylcellulose, sodium carboxymethylcellulose, and other well-known suspending agents.
The compounds of the present invention may be formulated for parenteral administration (e.g., by injection, for example bolus injection or continuous infusion) and may be presented in unit dose form in ampoules, pre-filled syringes, small volume infusion or in multi-dose containers with an added preservative. The compositions may take such forms as suspensions, solutions, or emulsions in oily or aqueous vehicles, for example solutions in aqueous polyethylene glycol. Examples of oily or nonaqueous carriers, diluents, solvents or vehicles include propylene glycol, polyethylene glycol, vegetable oils (e.g., olive oil), and injectable organic esters (e.g., ethyl oleate), and may contain formulatory agents such as preserving, wetting, emulsifying or suspending, stabilizing and/or dispersing agents. Alternatively, the active ingredient may be in powder form, obtained by aseptic isolation of sterile solid or by lyophilisation from solution for constitution before use with a suitable vehicle, e.g., sterile, pyrogen-free water.
The compounds of the present invention may be formulated for topical administration to the epidermis as ointments, creams or lotions, or as a transdermal patch. Ointments and creams may, for example, be formulated with an aqueous or oily base with the addition of suitable thickening and/or gelling agents. Lotions may be formulated with an aqueous or oily base and will in general also containing one or more emulsifying agents, stabilizing agents, dispersing agents, suspending agents, thickening agents, or coloring agents. Formulations suitable for topical administration in the mouth include lozenges comprising active agents in a flavored base, usually sucrose and acacia or tragacanth; pastilles comprising the active ingredient in an inert base such as gelatin and glycerin or sucrose and acacia; and mouthwashes comprising the active ingredient in a suitable liquid carrier.
The compounds of the present invention may be formulated for administration as suppositories.
A low melting wax, such as a mixture of fatty acid glycerides or cocoa butter is first melted and the active component is dispersed homogeneously, for example, by stirring. The molten homogeneous mixture is then poured into convenient sized molds, allowed to cool, and to solidify.
The compounds of the present invention may be formulated for vaginal administration. Pessaries, tampons, creams, gels, pastes, foams or sprays containing in addition to the active ingredient such carriers as are known in the art to be appropriate.
The compounds of the present invention may be formulated for nasal administration. The solutions or suspensions are applied directly to the nasal cavity by conventional means, for example, with a dropper, pipette or spray. The formulations may be provided in a single or multidose form. In the latter case of a dropper or pipette, this may be achieved by the patient administering an appropriate, predetermined volume of the solution or suspension. In the case of a spray, this may be achieved for example by means of a metering atomizing spray pump.
The compounds of the present invention may be formulated for aerosol administration, particularly to the respiratory tract and including intranasal administration. The compound will generally have a small particle size for example of the order of five (5) microns or less. Such a particle size may be obtained by means known in the art, for example by micronization. The active ingredient is provided in a pressurized pack with a suitable propellant such as a chlorofluorocarbon (CFC), for example, dichlorodifluoromethane, trichlorofluoromethane, or dichlorotetrafluoroethane, or carbon dioxide or other suitable gas. The aerosol may conveniently also contain a surfactant such as lecithin. The dose of drug may be controlled by a metered valve. Alternatively the active ingredients may be provided in a form of a dry powder, for example a powder mix of the compound in a suitable powder base such as lactose, starch, starch derivatives such as hydroxypropylmethyl cellulose and polyvinylpyrrolidine (PVP). The powder carrier will form a gel in the nasal cavity. The powder composition may be presented in unit dose form for example in capsules or cartridges of e.g., gelatin or blister packs from which the powder may be administered by means of an inhaler.
When desired, formulations can be prepared with enteric coatings adapted for sustained or controlled release administration of the active ingredient. For example, the compounds of the present invention can be formulated in transdermal or subcutaneous drug delivery devices. These delivery systems are advantageous when sustained release of the compound is necessary and when patient compliance with a treatment regimen is crucial. Compounds in transdermal delivery systems are frequently attached to a skin-adhesive solid support. The compound of interest can also be combined with a penetration enhancer, e.g., Azone (1-dodecylaza-cycloheptan-2-one). Sustained release delivery systems are inserted subcutaneously into to the subdermal layer by surgery or injection. The subdermal implants encapsulate the compound in a lipid soluble membrane, e.g., silicone rubber, or a biodegradable polymer, e.g., polylactic acid.
Suitable formulations along with pharmaceutical carriers, diluents and excipients are described in Remington: The Science and Practice of Pharmacy 1995, edited by E. W. Martin, Mack Publishing Company, 19th edition, Easton, Pa. A skilled formulation scientist may modify the formulations within the teachings of the specification to provide numerous formulations for a particular route of administration without rendering the compositions of the present invention unstable or compromising their therapeutic activity.
The modification of the present compounds to render them more soluble in water or other vehicle, for example, may be easily accomplished by minor modifications (salt formulation, esterification, etc.), which are well within the ordinary skill in the art. It is also well within the ordinary skill of the art to modify the route of administration and dosage regimen of a particular compound in order to manage the pharmacokinetics of the present compounds for maximum beneficial effect in patients.
The term “therapeutically effective amount” as used herein means an amount required to reduce symptoms of the disease in an individual. The dose will be adjusted to the individual requirements in each particular case. That dosage can vary within wide limits depending upon numerous factors such as the severity of the disease to be treated, the age and general health condition of the patient, other medicaments with which the patient is being treated, the route and form of administration and the preferences and experience of the medical practitioner involved. For oral administration, a daily dosage of between about 0.01 and about 1000 mg/kg body weight per day should be appropriate in monotherapy and/or in combination therapy. A preferred daily dosage is between about 0.1 and about 500 mg/kg body weight, more preferred 0.1 and about 100 mg/kg body weight and most preferred 1.0 and about 10 mg/kg body weight per day. Thus, for administration to a 70 kg person, the dosage range would be about 7 mg to 0.7 g per day. The daily dosage can be administered as a single dosage or in divided dosages, typically between 1 and 5 dosages per day. Generally, treatment is initiated with smaller dosages which are less than the optimum dose of the compound. Thereafter, the dosage is increased by small increments until the optimum effect for the individual patient is reached. One of ordinary skill in treating diseases described herein will be able, without undue experimentation and in reliance on personal knowledge, experience and the disclosures of this application, to ascertain a therapeutically effective amount of the compounds of the present invention for a given disease and patient.
The pharmaceutical preparations are preferably in unit dosage forms. In such form, the preparation is subdivided into unit doses containing appropriate quantities of the active component. The unit dosage form can be a packaged preparation, the package containing discrete quantities of preparation, such as packeted tablets, capsules, and powders in vials or ampoules. Also, the unit dosage form can be a capsule, tablet, cachet, or lozenge itself, or it can be the appropriate number of any of these in packaged form.
Indications and Method of Treatment
Indications
The application provides a method for preventing a Hepatitis C Virus (HCV) infection comprising administering to a patient in need thereof a therapeutically effective amount of a compound of Formula I.
The application provides the above method, further comprising administering to a patient in need thereof a therapeutically effective amount of an immune system suppressant.
The application provides a method for treating a Hepatitis C Virus (HCV) infection comprising administering to a patient in need thereof a therapeutically effective amount of a compound of Formula I.
The application provides any of the above methods, further comprising administering an immune system modulator or an antiviral agent that inhibits replication of HCV, or a combination thereof.
The application provides the above method, wherein the immune system modulator is an interferon or a chemically derivatized interferon.
The application provides any of the above methods, further comprising administering an immune system modulator or an antiviral agent that inhibits replication of HCV, or a combination thereof, wherein the antiviral agent is selected from the group consisting of a HCV protease inhibitor, a HCV polymerase inhibitor, a HCV helicase inhibitor, a HCV NSSA inhibitor, or any combination thereof.
Combination Therapy
The compounds of the invention and their isomeric forms and pharmaceutically acceptable salts thereof are useful in treating and preventing HCV infection alone or when used in combination with other compounds targeting viral or cellular elements or functions involved in the HCV lifecycle. Classes of compounds useful in the invention include, without limitation, all classes of HCV antivirals.
For combination therapies, mechanistic classes of agents that can be useful when combined with the compounds of the invention include, for example, nucleoside and non-nucleoside inhibitors of the HCV polymerase, protease inhibitors, helicase inhibitors, NS4B inhibitors, NSSA inhibitors and medicinal agents that functionally inhibit the internal ribosomal entry site (IRES) and other medicaments that inhibit HCV cell attachment or virus entry, HCV RNA translation, HCV RNA transcription, replication or HCV maturation, assembly or virus release. Specific compounds in these classes and useful in the invention include, but are not limited to, macrocyclic, heterocyclic and linear HCV protease inhibitors such as telaprevir (VX-950), boceprevir (SCH-503034), narlaprevir (SCH-9005 18), ITMN-191 (R-7227), TMC-435350 (a.k.a. TMC-435), MK-7009, BI-201335, BI-2061 (ciluprevir), BMS-650032, ACH-1625, ACH-1095 (HCV NS4A protease co-factor inhibitor), VX-500, VX-813, PHX-1766, PHX2054, IDX-136, IDX-316, ABT-450 EP-0 13420 (and congeners) and VBY-376; the Nucleosidic HCV polymerase (replicase) inhibitors useful in the invention include, but are not limited to, R7128, PSI-785 1, IDX-184, IDX-102, R1479, UNX-08 189, PSI-6130, PSI-938 and PSI-879 and various other nucleoside and nucleotide analogs and HCV inhibitors including (but not limited to) those derived as 2′-C-methyl modified nucleos(t)ides, 4′-aza modified nucleos(t)ides, and 7′-deaza modified nucleos(t)ides. Non-nucleosidic HCV polymerase (replicase) inhibitors useful in the invention, include, but are not limited to, HCV-796, HCV-371, VCH-759, VCH-916, VCH-222, ANA-598, MK-3281, ABT-333, ABT-072, PF-00868554, BI-207127, GS-9190, A-837093, JKT-109, GL-59728 and GL-60667.
In addition, compounds of the invention can be used in combination with cyclophyllin and immunophyllin antagonists (e.g., without limitation, DEBIO compounds, NM-811 as well as cyclosporine and its derivatives), kinase inhibitors, inhibitors of heat shock proteins (e.g., HSP90 and HSP70), other immunomodulatory agents that can include, without limitation, interferons (-alpha, -beta, -omega, -gamma, -lambda or synthetic) such as Intron A, Roferon-A, Canferon-A300, Advaferon, Infergen, Humoferon, Sumiferon MP, Alfaferone, IFN-β, Feron and the like; polyethylene glycol derivatized (pegylated) interferon compounds, such as PEG interferon-α-2a (Pegasys), PEG interferon-α-2b (PEGlntron), pegylated IFN-α-con1 and the like; long acting formulations and derivatizations of interferon compounds such as the albumin-fused interferon, Albuferon, Locteron, and the like; interferons with various types of controlled delivery systems (e.g., ITCA-638, omega-interferon delivered by the DUROS subcutaneous delivery system); compounds that stimulate the synthesis of interferon in cells, such as resiquimod and the like; interleukins; compounds that enhance the development of type 1 helper T cell response, such as SCV-07 and the like; TOLL-like receptor agonists such as CpG-10101 (actilon), isotorabine, ANA773 and the like; thymosin α-1; ANA-245 and ANA-246; histamine dihydrochloride; propagermanium; tetrachlorodecaoxide; ampligen; IMP-321; KRN-7000; antibodies, such as civacir, XTL-6865 and the like and prophylactic and therapeutic vaccines such as InnoVac C, HCV E1E2/MF59 and the like. In addition, any of the above-described methods involving administering an NS5A inhibitor, a Type I interferon receptor agonist (e.g., an IFN-α) and a Type II interferon receptor agonist (e.g., an IFN-γ) can be augmented by administration of an effective amount of a TNF-α antagonist. Exemplary, non-limiting TNF-α antagonists that are suitable for use in such combination therapies include ENBREL, REMICADE, and HUMIRA.
In addition, compounds of the invention can be used in combination with antiprotozoans and other antivirals thought to be effective in the treatment of HCV infection such as, without limitation, the prodrug nitazoxanide. Nitazoxanide can be used as an agent in combination with the compounds disclosed in this invention as well as in combination with other agents useful in treating HCV infection such as peginterferon α-2a and ribavirin.
Compounds of the invention can also be used with alternative forms of interferons and pegylated interferons, ribavirin or its analogs (e.g., tarabavarin, levoviron), microRNA, small interfering RNA compounds (e.g., SIRPLEX-140-N and the like), nucleotide or nucleoside analogs, immunoglobulins, hepatoprotectants, anti-inflammatory agents and other inhibitors of NS5A. Inhibitors of other targets in the HCV lifecycle include NS3 helicase inhibitors; NS4A co-factor inhibitors; antisense oligonucleotide inhibitors, such as ISIS-14803, AVI-4065 and the like; vector-encoded short hairpin RNA (shRNA); HCV specific ribozymes such as heptazyme, RPI, 13919 and the like; entry inhibitors such as HepeX-C, HuMax-HepC and the like; alpha glucosidase inhibitors such as celgosivir, UT-231B and the like; KPE-02003002 and BIVN 401 and IMPDH inhibitors. Other illustrative HCV inhibitor compounds include those disclosed in the following publications: U.S. Pat. Nos. 5,807,876; 6,498,178; 6,344,465; and 6,054,472; PCT Patent Application Publication Nos. WO97/40028; WO98/4038 1; WO00/56331, WO02/04425; WO03/007945; WO03/010141; WO03/000254; WO01/32153; WO00/06529; WO00/18231; WO00/10573; WO00/13708; WO01/85172; WO03/037893; WO03/037894; WO03/037895; WO02/100851; WO02/100846; WO99/01582; WO00/09543; WO02/18369; WO98/17679, WO00/056331; WO98/22496; WO99/07734; WO05/073216, WO05/073195 and WO08/021927.
Additionally, combinations of, for example, ribavirin and interferon, may be administered as multiple combination therapy with at least one of the compounds of the invention. The present invention is not limited to the aforementioned classes or compounds and contemplates known and new compounds and combinations of biologically active agents. It is intended that combination therapies of the present invention include any chemically compatible combination of a compound of this inventive group with other compounds of the inventive group or other compounds outside of the inventive group, as long as the combination does not eliminate the anti-viral activity of the compound of this inventive group or the anti-viral activity of the pharmaceutical composition itself.
Combination therapy can be sequential, that is treatment with one agent first and then a second agent (for example, where each treatment comprises a different compound of the invention or where one treatment comprises a compound of the invention and the other comprises one or more biologically active agents) or it can be treatment with both agents at the same time (concurrently). Sequential therapy can include a reasonable time after the completion of the first therapy before beginning the second therapy. Treatment with both agents at the same time can be in the same daily dose or in separate doses. Combination therapy need not be limited to two agents and may include three or more agents. The dosages for both concurrent and sequential combination therapy will depend on absorption, distribution, metabolism and excretion rates of the components of the combination therapy as well as other factors known to one of skill in the art. Dosage values will also vary with the severity of the condition to be alleviated. It is to be further understood that for any particular subject, specific dosage regimens and schedules may be adjusted over time according to the individual's need and the judgment of the one skilled in the art administering or supervising the administration of the combination therapy.
The application provides a method for preventing a Hepatitis C Virus (HCV) infection comprising administering to a patient in need thereof a therapeutically effective amount of a compound of Formula I.
The application provides the above method, further comprising administering to a patient in need thereof a therapeutically effective amount of an immune system suppressant.
The application provides a method for treating a Hepatitis C Virus (HCV) infection comprising administering to a patient in need thereof a therapeutically effective amount of a compound of Formula I.
The application provides any of the above methods, further comprising administering an immune system modulator or an antiviral agent that inhibits replication of HCV, or a combination thereof.
The application provides the above method, wherein the immune system modulator is an interferon or a chemically derivatized interferon.
The application provides any of the above methods, further comprising administering an immune system modulator or an antiviral agent that inhibits replication of HCV, or a combination thereof, wherein the antiviral agent is selected from the group consisting of a HCV protease inhibitor, a HCV polymerase inhibitor, a HCV helicase inhibitor, a HCV NS5A inhibitor, or any combination thereof.
Commonly used abbreviations include: acetyl (Ac), azo-bis-isobutyrylnitrile (AIBN), atmospheres (Atm), 9-borabicyclo[3.3.1]nonane (9-BBN or BBN), 2,2′-bis(diphenylphosphino)-1,1′-binaphthyl (BINAP), tert-butoxycarbonyl (Boc), di-tert-butyl pyrocarbonate or boc anhydride (BOC2O), benzyl (Bn), butyl (Bu), Chemical Abstracts Registration Number (CASRN), benzyloxycarbonyl (CBZ or Z), carbonyl diimidazole (CDI), 1,4-diazabicyclo[2.2.2]octane (DABCO), diethylaminosulfur trifluoride (DAST), dibenzylideneacetone (dba), 1,5-diazabicyclo[4.3.0]non-5-ene (DBN), 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), N,N′-dicyclohexylcarbodiimide (DCC), 1,2-dichloroethane (DCE), dichloromethane (DCM), 2,3-Dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), diethyl azodicarboxylate (DEAD), di-iso-propylazodicarboxylate (DIAD), di-iso-butylaluminumhydride (DIBAL or DIBAL-H), di-iso-propylethylamine (DIPEA), N,N-dimethyl acetamide (DMA), 4-N,N-dimethylaminopyridine (DMAP), N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,1′-bis-(diphenylphosphino)ethane (dppe), 1,1′-bis-(diphenylphosphino)ferrocene (dppf), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCI), 2-ethoxy-1-ethoxycarbonyl-1,2-dihydroquinoline (EEDQ), ethyl (Et), ethyl acetate (EtOAc), ethanol (EtOH), 2-ethoxy-2H-quinoline-1-carboxylic acid ethyl ester (EEDQ), diethyl ether (Et2O), ethyl isopropyl ether (EtOiPr), O-(7-azabenzotriazole-1-yl)-N, N,N′N′-tetramethyluronium hexafluorophosphate acetic acid (HATU), acetic acid (HOAc), 1-N-hydroxybenzotriazole (HOBt), high pressure liquid chromatography (HPLC), iso-propanol (IPA), isopropylmagnesium chloride (iPrMgCl), hexamethyl disilazane (HMDS), liquid chromatography mass spectrometry (LCMS), lithium hexamethyl disilazane (LiHMDS), meta-chloroperoxybenzoic acid (m-CPBA), methanol (MeOH), melting point (mp), MeSO2— (mesyl or Ms), methyl (Me), acetonitrile (MeCN), m-chloroperbenzoic acid (MCPBA), mass spectrum (ms), methyl t-butyl ether (MTBE), methyl tetrahydrofuran (MeTHF), N-bromosuccinimide (NBS), n-Butyllithium (nBuLi), N-carboxyanhydride (NCA), N-chlorosuccinimide (NCS), N-methylmorpholine (NMM), N-methylpyrrolidone (NMP), pyridinium chlorochromate (PCC), Dichloro-((bis-diphenylphosphino)ferrocenyl) palladium(II) (Pd(dppf)Cl2), palladium(II) acetate (Pd(OAc)2), tris(dibenzylideneacetone)dipalladium(0) (Pd2(dba)3), pyridinium dichromate (PDC), phenyl (Ph), propyl (Pr), iso-propyl (i-Pr), pounds per square inch (psi), pyridine (pyr), 1,2,3,4,5-Pentaphenyl-1′-(di-tert-butylphosphino)ferrocene (Q-Phos), room temperature (ambient temperature, rt or RT), sec-Butyllithium (sBuLi), tert-butyldimethylsilyl or t-BuMe2Si (TBDMS), tetra-n-butylammonium fluoride (TBAF), triethylamine (TEA or Et3N), 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO), triflate or CF3SO2— (Tf), trifluoroacetic acid (TFA), 1,1′-bis-2,2,6,6-tetramethylheptane-2,6-dione (TMHD), O-benzotriazol-1-yl-N,N,N′,N′-tetramethyluronium tetrafluoroborate (TBTU), thin layer chromatography (TLC), tetrahydrofuran (THF), trimethylsilyl or Me3Si (TMS), p-toluenesulfonic acid monohydrate (TsOH or pTsOH), 4-Me-C6H4SO2— or tosyl (Ts), and N-urethane-N-carboxyanhydride (UNCA). Conventional nomenclature including the prefixes normal (n), iso (i-), secondary (sec-), tertiary (tert-) and neo have their customary meaning when used with an alkyl moiety. (J. Rigaudy and D. P. Klesney, Nomenclature in Organic Chemistry, IUPAC 1979 Pergamon Press, Oxford.).
Compounds of the invention can be made by a variety of methods depicted in the illustrative synthetic reactions described below in the Examples section.
The starting materials and reagents used in preparing these compounds generally are either available from commercial suppliers, such as Aldrich Chemical Co., or are prepared by methods known to those skilled in the art following procedures set forth in references such as Fieser and Fieser's Reagents for Organic Synthesis; Wiley & Sons: New York, 1991, Volumes 1-15; Rodd's Chemistry of Carbon Compounds, Elsevier Science Publishers, 1989, Volumes 1-5 and Supplementals; and Organic Reactions, Wiley & Sons: New York, 1991, Volumes 1-40. It should be appreciated that the synthetic reaction schemes shown in the Examples section are merely illustrative of some methods by which the compounds of the invention can be synthesized, and various modifications to these synthetic reaction schemes can be made and will be suggested to one skilled in the art having referred to the disclosure contained in this application.
The starting materials and the intermediates of the synthetic reaction schemes can be isolated and purified if desired using conventional techniques, including but not limited to, filtration, distillation, crystallization, chromatography, and the like. Such materials can be characterized using conventional means, including physical constants and spectral data.
Unless specified to the contrary, the reactions described herein are typically conducted under an inert atmosphere at atmospheric pressure at a reaction temperature range of from about −78° C. to about 150° C., often from about 0° C. to about 125° C., and more often and conveniently at about room (or ambient) temperature, e.g., about 20° C.
Various substituents on the compounds of the invention can be present in the starting compounds, added to any one of the intermediates or added after formation of the final products by known methods of substitution or conversion reactions. If the substituents themselves are reactive, then the substituents can themselves be protected according to the techniques known in the art. A variety of protecting groups are known in the art, and can be employed. Examples of many of the possible groups can be found in “Protective Groups in Organic Synthesis” by Green et al., John Wiley and Sons, 1999. For example, nitro groups can be added by nitration and the nitro group can be converted to other groups, such as amino by reduction, and halogen by diazotization of the amino group and replacement of the diazo group with halogen. Acyl groups can be added by Friedel-Crafts acylation. The acyl groups can then be transformed to the corresponding alkyl groups by various methods, including the Wolff-Kishner reduction and Clemmenson reduction. Amino groups can be alkylated to form mono- and di-alkylamino groups; and mercapto and hydroxy groups can be alkylated to form corresponding ethers. Primary alcohols can be oxidized by oxidizing agents known in the art to form carboxylic acids or aldehydes, and secondary alcohols can be oxidized to form ketones. Thus, substitution or alteration reactions can be employed to provide a variety of substituents throughout the molecule of the starting material, intermediates, or the final product, including isolated products.
To a solution of ammonium thiocyanate (587 mg, 7.72 mmol, Eq: 1.25) in acetone (5 mL), was added acetyl chloride (607 mg, 550 μl, 7.74 mmol, Eq: 1.25). The reaction mixture was stirred for 1 hr, and the solid was filtered off. The filtrate was added to a solution of 3,5-dichloroaniline (1 g, 6.17 mmol, Eq: 1.00) in acetone (5 mL) and heated at reflux for 6 hr, then cooled to room temperature and stirred o/n. The resulting suspension was concentrated and chromatographed (40 g Analogix, 100 hexane to 10% ethyl acetate/hexane) to give 1.128 g (70%) of desired product as a white solid.
A solution of N-(3,5-dichlorophenylcarbamothioyl)acetamide (300 mg, 1.14 mmol, Eq: 1.00), diiodomethane (916 mg, 276 μl, 3.42 mmol, Eq: 3), and triethylamine (346 mg, 477 μl, 3.42 mmol, Eq: 3) in acetone (5 mL) was heated at reflux overnight. The resulting suspension was filtered. The filtrate was concentrated and chromatographed (25 g Thomson, 100% hexane to 10% ethyl acetate/hexane) to give 159 mg (51%) of desired product as a white solid, containing ˜1.7:1 ratio of starting material and product, which was carried onto the next step as a mixture.
A solution of (Z)-1-(2-(3,5-dichlorophenylimino)-1,3-thiazetidin-3-yl)ethanone (159 mg, 578 μmol, Eq: 0.956) and hydrazine (194 mg, 190 μL, 6.04 mmol, Eq: 10) in acetonitrile (5 mL) was heated at reflux. The reaction mixture was concentrated and chromatographed (11 g Supelco 100% CH2Cl2 to 5% methanol/CH2Cl2) to give 40 mg of solid containing desired product and other impurities. Further purification by preparative plate chromatography gave 20 mg (14%) of desired product as an off-white solid.
1H NMR (300MHz, DMSO) δ: 13.04 (s, 1H), 9.56 (s, 1H), 7.57 (d, J=2 Hz, 2H), 6.90 (t, J=2 Hz, 1H) ppm
To a solution of benzoyl isothiocyanate (1.01 g, 6.17 mmol, Eq: 1.00) in acetone (15 mL), was added 3,5-dichloroaniline (1 g, 6.17 mmol, Eq: 1.00). The reaction mixture was heated at 40° C. for 3 hr. TLC shows starting material. The reaction was heated to 50° C. overnight. The reaction was cooled to room temperature, and carefully poured into ice water (pH 5). The resulting sold was filtered, washed with water, and dried to give 1.85 g (92%) of desired product as an off-white solid.
A solution of N-(3,5-dichlorophenylcarbamothioyl)benzamide (1 g, 3.07 mmol, Eq: 1.00), diiodomethane (2.49 g, 750 μl, 9.3 mmol, Eq: 3.02), and triethylamine (944 mg, 1.3 ml, 9.33 mmol, Eq: 3.03) in acetone (15 mL) was heated at reflux overnight. The resulting suspension was filtered and the filtrate was concentrated and chromatographed (40 g Analogix 100% hexane to 10% ethyl acetate/hexane) to give 255 mg (25%) of desired product as a white solid.
A solution of (Z)-N-(3-(3,5-dichlorophenyl)-1,3-thiazetidin-2-ylidene)benzamide (318 mg, 943 μmol, Eq: 1.00) and hydrazine (302 mg, 296 μL, 9.43 mmol, Eq: 10) in acetonitrile (10 mL) was heated at 70° o/n. The reaction mixture was concentrated and chromatographed (23 g Supelco, 0 to 3% methanol/CH2Cl2) to give 81 mg (28%) of desired product as an off white solid. 1H NMR (300MHz, DMSO) δ: 13.90 (s, 1H), 9.81 (s, 1H), 7.95 (m, 2H), 7.64 (d, J=2 Hz, 2H), 7.53 (m, 3H), 6.93 (t, J=2 Hz, 1H) ppm
N-(3,5-dichlorophenylcarbamothioyl)-2-(1,3-dioxoisoindolin-2-yl)acetamide
Ammonium thiocyanate (705 mg, 9.26 mmol, Eq: 1.25) and 3,5-dichloroaniline (1.2 g, 7.41 mmol, Eq: 1.00) were dissolved in acetone (5 ml). 2-(1,3-dioxoisoindolin-2-yl)acetyl chloride (2.12 g, 9.11 mmol, Eq: 1.23) was added as solution in acetone (8 ml). The suspension was stirred for 50 min. The reaction mixture was concentrated in vacuo to afford 3.5 g of the desired product as an off white solid.
MS +m/z: 409.8 (M+1)
1H NMR (300 MHz, DMSO-d6) δ ppm 4.58 (s, 2H) 7.49 (t, J=1.89 Hz, 1H) 7.73 (d, J=1.89 Hz, 2H) 7.86-7.96 (m, 4H) 11.95 (s, 1H) 12.05 (s, 1H)
N-(3,5-dichlorophenylcarbamothioyl)-2-(1,3-dioxoisoindolin-2-yl)acetamide (500 mg, 1.1 mmol, Eq: 1.00), diiodomethane (886 mg, 267 μl, 3.31 mmol, Eq: 3) and triethylamine (335 mg, 461 μl, 3.31 mmol, Eq: 3) were dissolved in acetone (8 ml). The reaction mixture was heated to 80° C. for 17 hrs. The reaction was filtered and the filtrate was concentrated in vacuo to to afford 512 mg (55%) of the desired product as a light brown solid (512 mg) which was used without further purification.
MS +m/z: 421.8 (M+1)
2-(2-{2-[3,5-Dichloro-phenylimino]-[1,3]thiazetidin-3-yl}-2-oxo-ethyl)-isoindole-1,3-dione (512 mg, 609 μmol, Eq: 1.00) was suspended in acetonitrile (4 ml). Hydrazine (452 mg, 14.1 mmol, Eq: 23.2) was added and the reaction mixture was stirred at 90° C. for 90 min. The mixture was filtered and the solid washed with acetonitrile (20 ml). The resulting filter cake (419 mg) contains a by-product 2,3-Dihydro-phthalazine-1,4-dione. The crude material was purified by preparative HPLC (0.1%TFA in water/0.1% TFA in AcCN) 95% to 10% over 16 minutes to afford 9 mg (6%) of the desired product as a white solid.
MS +m/z: 258.0/260.0. (M+1)
1H NMR (300 MHz, MeOD) δ ppm 3.32 (dt, J=3.30, 1.56 Hz, 2H) 4.18 (s, 2 H) 5.51 (s, 1H) 6.94-7.01 (m, 1H) 7.58 (d, J=1.89 Hz, 2H)
In a 25 mL round-bottomed flask, 1-benzyl-3-bromo-5-fluoro-1H-1,2,4-triazole (940 mg, 3.67 mmol, Eq: 1.00) and N-bromosuccinimide (784 mg, 4.4 mmol, Eq: 1.20) were combined with CCl4 (15 ml) to give a white suspension. The reaction was heated to reflux under stirring by illumination with 250 W lamp. After 6 hours, (the reaction was closely monitored by FNMR) the mixture was cooled, filtered and concentrated. The residue was immediately dissolved in THF (9 mL), water (3 mL) was added and the mixture was stirred at room temperature overnight. The solution was diluted with Et2O (20 mL) and extracted with 2.5 N NaOH (10 mL×3). The combined aqueous phases were acidified with concentrate HCl solution to PH around 3, and extracted with EtOAc (3×20 mL), the EtOAc extractions were washed with brine and dried over anhydrous Na2SO4, filtered and concentrated to afford the product 550 mg (90%) FNMR (DMSO) 114.8.
In a 50 mL round-bottomed flask, sodium hydride (133 mg, 3.31 mmol, Eq: 1.00) was combined with DMF (10 mL) to give a white suspension. 1-(Chloromethyl)-4-methoxybenzene (675 mg, 4.31 mmol, Eq: 1.3) in DMF (5 ML) was added dropwise at 0° C. After the addition, reaction was stirred for 30 min at 0° C. 1-(Chloromethyl)-4-methoxybenzene (675 mg, 4.31 mmol, Eq: 1.3) in DMF (5 mL) was added dropwise, the resulting mixture was stirred at room temperature for overnight. The solution was added EtOAc (50 mL), washed with H2O (50 mL) and brine (50 mL), organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by column chromatography (hexanes/EtOAc =80/20) to give an oil 300 mg (32%).
In a 25 mL round bottle, 5-bromo-3-fluoro-1-(4-methoxybenzyl)-1H-1,2,4-triazole (200 mg, 699 μmol, Eq: 1.00) was combined with DMF (5.00 ml) to give a colorless solution. Sodium 2-methylpropan-2-olate (134 mg, 1.4 mmol, Eq: 2.00) and 3,5-dichloroaniline (136 mg, 839 μmol, Eq: 1.20) were added. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added EtOAc (30×2 mL) extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column chromatography (Hexanes/EtOAc=70/30) to afford the compound 50 mg (20%).
In a 10 mL round bottle, (3,5-dichloro-phenyl)-[5-fluoro-2-(4-methoxy-benzyl)-2H-[1,2,4]triazol-3-yThamine (50 mg, 136 μmol, Eq: 1.00) was combined with TFA (5.00 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 12 mg (36%). MH+ 247.1
In a 25 mL round bottle, 5-bromo-1-(4-methoxybenzyl)-3-(methoxymethyl)-1H-1,2,4-triazole (250 mg, 801 μmol, Eq: 1.00) was combined with DMF (5.00 ml) to give a colorless solution. 3,5-Dichloroaniline (156 mg, 961 μmol, Eq: 1.20) and sodium 2-methylpropan-2-olate (154 mg, 1.6 mmol, Eq: 2.00) were added. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added Ether (30×2 mL) extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column (Hexanes/EtOAc=70/30) to afford the compound 30 mg (9.5%). MH+ 393.0
In a 10 mL round bottle, (3,5-dichloro-phenyl)-[2-(4-methoxy-benzyl)-5-methoxymethyl-2H-[1,2,4]triazol-3-yThamine (30 mg, 76.3 μmol, Eq: 1.00) was combined with TFA (2.5 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 17 mg (82%). MH+ 273.0
In a 25 mL round bottle, 5-bromo-1-(4-methoxybenzyl)-3-(methoxymethyl)-1H-1,2,4-triazole (400 mg, 1.28 mmol, Eq: 1.00) was combined with DMF (8.00 ml) to give a colorless solution. 2,6-dichloropyridin-4-amine (251 mg, 1.54 mmol, Eq: 1.20) and sodium 2-methylpropan-2-olate (246 mg, 2.56 mmol, Eq: 2.00) were added. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added Ether (30×2 mL) extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column (Hexanes/EtOAc=70/30) to afford the compound 60 mg (12%). MH+ 394.0
In a 10 mL round bottle, (2,6-dichloro-pyridin-4-yl)-[2-(4-methoxy-benzyl)-5-methoxymethyl-2H-[1,2,4]triazol-3-yl]-amine (60 mg, 152 μmol, Eq: 1.00) was combined with TFA (2.5 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 28 mg (67%). MH+ 274.0
In a 25 mL round bottle, 3,5-dibromo-1-(4-methoxybenzyl)-1H-1,2,4-triazole (1 g, 2.88 mmol, Eq: 1.00) was combined with DMF (8.00 ml) to give a colorless solution. 3,5-Dibromo-1-(4-methoxybenzyl)-1H-1,2,4-triazole (1 g, 2.88 mmol, Eq: 1.00) and sodium 2-methylpropan-2-olate (554 mg, 5.76 mmol, Eq: 2.00) were added. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added Ether (30×2 mL) to extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column (Hexanes/EtOAc=70/30) to afford the compound 380 mg (31%). MH+ 428.8
In 10 mL round bottle, [5-bromo-2-(4-methoxy-benzyl)-2H-[1,2,4]triazol-3-yl]-(3,5-dichloro-phenyl)-amine (380 mg, 888 μmol, Eq: 1.00) was combined with TFA (2.5 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 230 mg (84%). MH+ 308.8
In a 100 mL round-bottomed flask, 3-bromo-5-methoxy-1H-1,2,4-triazole (650 mg, 2.19 mmol, Eq: 1.00), 1-(chloromethyl)-4-methoxybenzene (343 mg, 2.19 mmol, Eq: 1) and N-ethyl-N-isopropylpropan-2-amine (566 mg, 4.38 mmol, Eq: 2) were combined with acetonitrile (101 ml) to give a light yellow solution. Potassium iodide (182 mg, 1.1 mmol, Eq: 0.5) was added. The mixture was heated to reflux for 2 hours. The mixture was cooled down and poured into water (50 mL) and extracted with EtOAc (3×50 mL), the combined extracts were washed with water (50 mL) and brine (50 mL), dried over anhydrous Na2SO4, concentrate to afford the crude product. Purify the compound by column chromatography (hexanes/EtOAc=70/30) to afford the oil 0.13 g (20%).
In a 25 mL round bottle, 5-bromo-3-methoxy-1-(4-methoxybenzyl)-1H-1,2,4-triazole (130 mg, 436 μmol, Eq: 1.00) was combined with DMF (2.5 mL) to give a colorless solution. 3,5-Dichloroaniline (84.8 mg, 523 μmol, Eq: 1.20) and sodium 2-methylpropan-2-olate (83.8 mg, 872 μmol, Eq: 2.00) were added. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added Ether (30×2 mL) to extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column (Hexanes/EtOAc=70/30) to afford the compound 50 mg (30%). MH+ 378.9
In a 10 mL round bottle, 3,5-dichloro-phenyl)-[5-methoxy-2-(4-methoxy-benzyl)-2H-[1,2,4]triazol-3-yl]-amine (50 mg, 132 μmol, Eq: 1.00) was combined with TFA (5 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 18 mg (53%). MH+ 259.0
(2,6-Dichloro-pyridin-4-yl)-[5-methoxy-2-(4-methoxy-benzyl)-2H-[1,2,4]triazol-3-yl]-amine
In a 25 mL round bottle, 5-bromo-3-methoxy-1-(4-methoxybenzyl)-1H-1,2,4-triazole (170 mg, 570 μmol, Eq: 1.00) was combined with DMF (2.5 mL) to give a colorless solution. 2,6-Dichloro-pyridin-4-ylamine (93 mg, 570 μmol, Eq: 1.00) and sodium 2-methylpropan-2-olate (110 mg, 1.14 mmol, Eq: 2.00) were added. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added Ether (30×2 mL) to extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column (Hexanes/EtOAc=70/30) to afford the compound 120 mg (55%). MH+ 380.0
In 10 mL round bottle, (2,6-dichloro-pyridin-4-yl)-[5-methoxy-2-(4-methoxy-benzyl)-2H-[1,2,4]triazol-3-yl]-amine (120 mg, 316 μmol, Eq: 1.00) was combined with TFA (5.0 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 42 mg (51%). MH+ 259.8
4-[5-Methoxy-2-(4-methoxy-benzyl)-2H-[1,2,4]triazol-3-ylamino]-2-trifluoromethyl-benzonitrile
In a 25 mL round bottle, 5-bromo-3-methoxy-1-(4-methoxybenzyl)-1H-1,2,4-triazole (170 mg, 570 μmol, Eq: 1.00) was combined with DMF (2.95 ml) to give a colorless solution. 4-Amino-2-(trifluoromethyl)benzonitrile (106 mg, 570 μmol, Eq: 1.00) and sodium 2-methylpropan-2-olate (110 mg, 1.14 mmol, Eq: 2.00) were added. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added Ether (30×2 mL) to extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column (Hexanes/EtOAc=70/30) to afford the compound 100mg (44%). MH+ 404.1
In a 10 mL round bottle, 4-[5-methoxy-2-(4-methoxy-benzyl)-2H-[1,2,4]triazol-3-ylamino]-2-trifluoromethyl-benzonitrile (100 mg, 248 μmol, Eq: 1.00) was combined with TFA (5.0 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 32 mg (46%). MH+ 283.9
In a 25 mL round bottle, 5-bromo-3-methoxy-1-(4-methoxybenzyl)-1H-1,2,4-triazole (170 mg, 570 μmol, Eq: 1.00) was combined with DMF (2.95 ml) to give a colorless solution. 4-Bromo-3,5-dichloroaniline (137 mg, 570 μmol, Eq: 1.00) and sodium 2-methylpropan-2-olate (110 mg, 1.14 mmol, Eq: 2.00) were added. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added Ether (30×2 mL) to extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column (Hexanes/EtOAc=70/30) to afford the compound 90mg (35%). MH+ 459.0
In a 10 mL round bottle, (4-bromo-3,5-dichloro-phenyl)-[5-methoxy-2-(4-methoxy-benzyl)-2H-[1,2,4]triazol-3-yl]-amine (90 mg, 196 μmol, Eq: 1.00) was combined with TFA (5.0 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 35 mg (53%). MH+ 338.8
In a 25 mL round bottle, 3,5-dichloro-1-(4-methoxybenzyl)-1H-1,2,4-triazole (300 mg, 1.16 mmol, Eq: 1.00) was combined with DMF (2.6 ml) to give a colorless solution. 3,5-Dichloroaniline (188 mg, 1.16 mmol, Eq: 1.00) and sodium 2-methylpropan-2-olate (223 mg, 2.32 mmol, Eq: 2.00) were added. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added Ether (30×2 mL) to extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column (Hexanes/EtOAc=70/30) to afford the compound 360 mg (81%). MH+ 384.0
In a 10 mL round bottle, [5-chloro-2-(4-methoxy-benzyl)-2H-[1,2,4]triazol-3-yl]-(3,5-dichloro-phenyl)-amine (360 mg, 938 μmol, Eq: 1.00) was combined with TFA (5.0 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 183 mg (74%). MH+ 264.8
In a 100 mL round-bottomed flask, 3-chloro-5-(methylsulfonyl)-1H-1,2,4-triazole (1 g, 5.51 mmol, Eq: 1.00), 1-(chloromethyl)-4-methoxybenzene (862 mg, 5.51 mmol, Eq: 1) and N-ethyl-N-isopropylpropan-2-amine (1.42 g, 11.0 mmol, Eq: 2) were combined with acetonitrile (50 mL) to give a light yellow solution. Potassium iodide (457 mg, 2.75 mmol, Eq: 0.5) was added. The mixture was heated to reflux for 2 hours. The mixture was cooled down and poured into water (50 mL) and extracted with EtOAc (3×50 mL), the combined extracts were washed with water (50 mL) and brine (50 mL), dried over anhydrous Na2SO4, concentrate to afford the crude product. Purify the compound by column chromatography (hexanes/EtOAc=70/30) to afford the oil 0.98 g (59%).
In a 25 mL round bottle, 5-chloro-1-(4-methoxybenzyl)-3-(methylsulfonyl)-1H-1,2,4-triazole (200 mg, 663 μmol, Eq: 1.00) was combined with DMF (2.6 ml) to give a colorless solution. 3,5-Dichloroaniline (107 mg, 663 μmol, Eq: 1.00) and sodium 2-methylpropan-2-olate (127 mg, 1.33 mmol, Eq: 2.00) were added. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added Ether (30×2 mL) to extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column (Hexanes/EtOAc=70/30) to afford the compound 150 mg (53%). MH+ 427.0
In a 10 mL round bottle, (3,5-dichloro-phenyl)-[5-methanesulfonyl-2-(4-methoxy-benzyl)-2H-[1,2,4]triazol-3-yl]-amine (150 mg, 351 μmol, Eq: 1.00) was combined with TFA (5 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 25 mg (23%). MH+ 306.9
In a 25 mL round bottle, 5-chloro-1-(4-methoxybenzyl)-3-(methylsulfonyl)-1H-1,2,4-triazole (200 mg, 663 μmol, Eq: 1.00) was combined with DMF (3 mL) to give a colorless solution. 4-Bromo-3,5-dichloroaniline (160 mg, 663 μmol, Eq: 1.00) and sodium 2-methylpropan-2-olate (127 mg, 1.33 mmol, Eq: 2.00) were added. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added Ether (30×2 mL) to extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column (Hexanes/EtOAc=70/30) to afford the compound 180 mg (54%). MH+ 507.0
In a 10 mL round bottle, (4-Bromo-3,5-dichloro-phenyl)-[5-methanesulfonyl-2-(4-methoxy-benzyl)-2H-[1,2,4]triazol-3-yl]-amine (180 mg, 356 μmol, Eq: 1.00) was combined with TFA (5 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 80 mg (58%). MH+ 386.8
In a 100 mL round-bottomed flask, 3-bromo-N,N-dimethyl-1H-1,2,4-triazol-5-amine (1.5 g, 7.85 mmol, Eq: 1), 1-(chloromethyl)-4-methoxybenzene (1.23 g, 7.85 mmol, Eq: 1) and N-ethyl-N-isopropylpropan-2-amine (2.03 g, 15.7 mmol, Eq: 2) were combined with acetonitrile (50 mL) to give a light yellow solution. Potassium iodide (652 mg, 3.93 mmol, Eq: 0.5) was added. The mixture was heated to reflux for 2 hours. The mixture was cooled down and poured into water (50 mL) and extracted with EtOAc (3×50 mL), the combined extracts were washed with water (50 mL) and brine (50 mL), dried over anhydrous Na2SO4, concentrate to afford the crude product. Purify the compound by column chromatography (hexanes/EtOAc=70/30) to afford the oil 2.0 g (82%). MH+312.9
In a 25 mL round bottle, 5-bromo-1-(4-methoxybenzyl)-N,N-dimethyl-1H-1,2,4-triazol-3-amine (1 g, 3.21 mmol, Eq: 1.00) was combined with DMF (18.4 ml) to give a colorless solution. 3,5-dichloroaniline (521 mg, 3.21 mmol, Eq: 1.00) and sodium 2-methylpropan-2-olate (618 mg, 6.43 mmol, Eq: 2.00) were added. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added Ether (30×2 mL) to extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column (Hexanes/EtOAc=70/30) to afford the compound 40mg (3%). MH+ 392.1
In a 10 mL round bottle, N*5*-(3,5-dichloro-phenyl)-1-(4-methoxy-benzyl)-N*3*,N*3*-dimethyl-1H-[1,2,4]triazole-3,5-diamine (52 mg, 133 μmol, Eq: 1.00) was combined with TFA (5 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 23 mg (64%). MH+ 273.8
In a 25 mL round bottle, 5-bromo-1-(4-methoxybenzyl)-N,N-dimethyl-1H-1,2,4-triazol-3-amine (1 g, 3.21 mmol, Eq: 1.00) was combined with DMF (18.4 ml) to give a colorless solution. 4-bromo-3,5-dichloroaniline (774 mg, 3.21 mmol, Eq: 1.00) and sodium 2-methylpropan-2-olate (618 mg, 6.43 mmol, Eq: 2.00) were added. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added EtOAc (30×2 mL) to extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column (Hexanes/EtOAc=70/30) to afford the compound 40 mg (3%). MH+ 472.0
In a 10 mL round bottle, N*5*-(4-bromo-3,5-dichloro-phenyl)-1-(4-methoxy-benzyl)-N*3*,N*3*-dimethyl-1H-[1,2,4]triazole-3,5-diamine (55 mg, 117 μmol, Eq: 1.00) was combined with TFA (5 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 15 mg (37%). MH+ 351.9
In a 100 mL round-bottomed flask, 3-bromo-1H-1,2,4-triazole (3 g, 20.3 mmol, Eq: 1.00), 1-(chloromethyl)-4-methoxybenzene (3.18 g, 20.3 mmol, Eq: 1) and N-ethyl-N-isopropylpropan-2-amine (5.24 g, 40.6 mmol, Eq: 2) were combined with acetonitrile (101 ml) to give a light yellow solution. Potassium iodide (1.68 g, 10.1 mmol, Eq: 0.5) was added. The mixture was heated to reflux for 2 hours. The mixture was cooled down and poured into water (50 mL) and extracted with EtOAc (3×50 mL), the combined extracts were washed with water (50 mL) and brine (50 mL), dried over anhydrous Na2SO4, concentrate to afford the crude product. Purify the compound by column chromatography (hexanes/EtOAc=70/30) to afford the oil 1.1 g (20%).
In a 25 mL round bottle, 5-bromo-1-(4-methoxybenzyl)-1H-1,2,4-triazole (200 mg, 746 μmol, Eq: 1.00) was combined with DMF (9.18 ml) to give a colorless solution. 3,4,5-Trichloroaniline (147 mg, 746 μmol, Eq: 1.00) and sodium 2-methylpropan-2-olate (143 mg, 1.49 mmol, Eq: 2.00) were added. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added Ether (30×2 mL) to extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column (Hexanes/EtOAc=70/30) to afford the compound 60 mg (21%). MH+ 385.0
In a 10 mL round bottle, [2-(4-methoxy-benzyl)-2H-[1,2,4]triazol-3-yl]-(3,4,5-trichloro-phenyl)-amine (60 mg, 156 μmol, Eq: 1.00) was combined with TFA (5 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 18 mg (44%). MH+ 264.8
(4-Bromo-3,5-dichloro-phenyl)-[2-(4-methoxy-benzyl)-2H-[1,2,4]triazol-3-yl]-amine
In a 10 mL round-bottomed flask, sodium 2-methylpropan-2-olate (108 mg, 1.12 mmol, Eq: 1.5), 3-bromo-1-(4-methoxybenzyl)-1H-1,2,4-triazole (200 mg, 746 μmol, Eq: 1.00) and 4-bromo-3,5-dichloroaniline (180 mg, 746 μmol, Eq: 1.00) were combined with DMF (3 ml) to give a dark brown suspension. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added Ether (30×2 mL) to extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column (Hexanes/EtOAc=70/30) to afford the compound 58mg (18%). MH+ 429.0
In a 10 mL round bottle, (4-bromo-3,5-dichloro-phenyl)-[2-(4-methoxy-benzyl)-2H-[1,2,4]triazol-3-yThamine (58 mg, 135 μmol, Eq: 1.00) was combined with TFA (5.00 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 15 mg (36%). MH+ 308.8
In a 10 mL sealed tube, 5-bromo-1-(4-methoxybenzyl)-3-nitro-1H-1,2,4-triazole (1 g, 3.19 mmol, Eq: 1.00) and 1-(4-methoxyphenyl)-N-methylmethanamine (966 mg, 6.39 mmol, Eq: 2.00) were combined, the mixture was heated to 150° C. for overnight. Cool the reaction down, added CH2Cl2 (50 mL) washed with H2O (50 mL) and brine (50 mL), the organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure to afford the crude product 1.15 g (94% crude). MH+ 490.3
In a 100 mL round bottle, N,1-bis(4-methoxybenzyl)-N-methyl-3-nitro-1H-1,2,4-triazol-5-amine (1100 mg, 2.87 mmol, Eq: 1.00) and zinc (938 mg, 14.3 mmol, Eq: 5.00) were combined with the solution of saturated NH4Cl aqueous solution/THF (1:1) (60.0 ml), the mixture was stirred at room temperature for 1 hour. Filter out the solid, extracted the mixture with CH2Cl2 (50 mL×2), the organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by column chromatography to give a light yellow solid 0.95 g (94%). MH+ 354.1
In a 25 mL sealed tube, sodium tert-butoxide (97.9 mg, 1.02 mmol, Eq: 1.20), bis(dibenzylideneacetone)palladium (48.8 mg, 84.9 μmol, Eq: 0.1) and 2-di-tert-butylphosphino-2′,4′,6′-triisopropylbiphenyl (36.0 mg, 84.9 μmol, Eq: 0.1) were combined with toluene (5 mL) to give a dark brown suspension. N5,1-bis(4-methoxybenzyl)-N5-methyl-1H-1,2,4-triazole-3,5-diamine (300 mg, 849 μmol, Eq: 1.00) and 1-bromo-3,5-dichlorobenzene (230 mg, 1.02 mmol, Eq: 1.20) were added. The reaction mixture was degassed with argon for 15 min, and then heated to 110° C. for 3 hours. The reaction mixture was cooled and diluted with EtOAc (50 mL), washed with H2O (25 mL) and brine (25 mL). The organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure to yield an oil from which the compound was isolated by column chromatography (Hexanes/EtOAc=70/30) to give an off-white solid 140 mg (33%). MH+ 498.2
In a 10 mL round bottle, N*3*-(3,5-dichloro-phenyl)-1,N*5*-bis-(4-methoxy-benzyl)-N*5*-methyl-1H-[1,2,4]triazole-3,5-diamine (140 mg, 281 μmol, Eq: 1.00) was combined with TFA (5.00 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 30 mg (41%). MH+ 257.8
In a 25 mL round bottle, 5-bromo-1-(4-methoxybenzyl)-1H-1,2,4-triazole (250 mg, 932 μmol, Eq: 1.00) was combined with DMF (5 mL) to give a colorless solution. Sodium 2-methylpropan-2-olate (179 mg, 1.86 mmol, Eq: 2.00) and 4-amino-2,6-dichlorobenzonitrile (349 mg, 1.86 mmol, Eq: 2.00) were added. The reaction was degassed by nitrogen for 5 min. The resulting solution was heated to 85° C. overnight under nitrogen. The reaction mixture was cooled and diluted with 20 mL H2O, added Ether (30×2 mL) to extract the product, dried the organic layer over anhydrous Na2SO4, concentrate the solution, purify the compound by column (Hexanes/EtOAc=70/30) to afford the compound 70 mg (20%). MH+ 374.0
In a 10 mL round bottle, 2,6-dichloro-4-[2-(4-methoxy-benzyl)-2H-[1,2,4]triazol-3-ylamino]-benzonitrile (70 mg, 187 μmol, Eq: 1.00) was combined with TFA (5.00 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 35 mg (74%). MH+ 253.8
In a 25 mL sealed tube, sodium 2-methylpropan-2-olate (81.6 mg, 849 μmol, Eq: 1.00), bis(dibenzylideneacetone)palladium (48.8 mg, 84.9 μmol, Eq: 0.1) 2-di-tert-butylphosphino-2′,4′,6′-triisopropylbiphenyl (36.0 mg, 84.9 μmol, Eq: 0.1) were combined with toluene (5.00 ml) to give a dark brown suspension. N5,1-bis(4-methoxybenzyl)-N5-methyl-1H-1,2,4-triazole-3,5-diamine (300 mg, 849 μmol, Eq: 1.00) and 4-bromo-2-(trifluoromethyl)benzonitrile (212 mg, 849 μmol, Eq: 1.00) were added. The reaction mixture was degassed with argon for 15 min, and then heated to 110° C. for 3 hours. The reaction mixture was cooled and diluted with EtOAc (50 mL), washed with H2O (25 mL) and brine (25 mL). The organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure to yield an oil from which the compound was isolated by column chromatography (Hexanes/EtOAc=70/30) to give an off-white solid 110 mg (25%). MH+ 523.1
In a 10 mL round bottle, 4-{1-(4-methoxy-benzyl)-5-[(4-methoxy-benzyl)-methyl-amino]-1H-[1,2,4]triazol-3-ylamino}-2-trifluoromethyl-benzonitrile (110 mg, 211 μmol, Eq: 1.00) was combined with TFA (5.00 mL) to give a colorless solution. The resulting solution was heated to 65° C. for 3 hours; the reaction mixture was concentrated, and then diluted with EtOAc (30 mL). The solution was washed with saturated NaHCO3, organic layer was dried over anhydrous MgSO4, filtered and volatiles were removed under reduced pressure. The compound was isolated by preparative TLC to give an off-white solid 45 mg (76%). MH+ 282.9
To a solution of 1-(3,5-dichlorophenyl)thiourea (1 g, 4.52 mmol, Eq: 1.00) in dichloromethane (13.2 g, 10.0 ml, 155 mmol, Eq: 34.4) at 0° C., was added trifluoroacetic anhydride (9.37 g, 6.3 ml, 44.6 mmol, Eq: 9.86). The solution was gradually warmed to room temperature and stirred o/n. The reaction mixture was concentrated and chromatographed (80 g Analogix, 100% hex to 10% EtOAc/hex) to give 952 mg (64%) of desired product as a light brown oil.
A solution of N-(3,5-dichlorophenylcarbamothioyl)-2,2,2-trifluoroacetamide (399 mg, 1.26 mmol, Eq: 1.00), diiodomethane (996 mg, 300 μl, 3.72 mmol, Eq: 2.96), and triethylamine (381 mg, 525 μl, 3.77 mmol, Eq: 2.99) in acetone was heated at reflux overnight. The resulting suspension was filtered and the filtrate was concentrated and chromatographed (40 g Analogix 100% hex to 10% EtOAc/hex) to give 55 mg (13%) of desired product as a white solid.
A solution of (Z)-N-(3-(3,5-dichlorophenyl)-1,3-thiazetidin-2-ylidene)-2,2,2-trifluoroacetamide (135 mg, 410 μmol, Eq: 1.00) and hydrazine (131 mg, 129 μL, 4.1 mmol, Eq: 10) in acetonitrile (3.93 g, 5 mL, 95.7 mmol, Eq: 233) was heated at 70 o/n. The reaction mixture was concentrated and the crude residue was purified by preparative TLC (5% MeOH/DCM) to give 44 mg (36%) of desired product as a light yellow solid.
1H NMR (300 MHz, DMSO) δ: 13.80 (s, 1H), 10.15 (s, 1H), 7.56 (d, J=2 Hz, 2H), 7.10 (m, 1H) ppm
To a solution of methoxide [in MeOH] (24.0 ml, 12.0 mmol, Eq: 1.01), was added 1H-1,2,4-triazol-5-amine (1.002 g, 11.9 mmol, Eq: 1.00). The reaction mixture was stirred until triazole had completely dissolved. The reaction was concentrated to give an orange oil, which was redissolved in DMF (13 mL) and to this solution was added (bromomethyl)benzene (2.04 g, 1.42 ml, 11.9 mmol, Eq: 1.00). Stirred at room temperature under an argon atmosphere for 3 hr. The residue (solid/oil) was triturated with hot CHCl3 and filtered. Concentrated under a stream of nitrogen and purified by flash chromatography (reverse phase, 5% to 100% acetonitrile in water (each containing 0.1% TFA)) in multiple runs. Combined the fractions containing the major component that eluted from each run. Concentrated under a stream of nitrogen to give 0.987 g of desired product in a 40:60 ratio.
A mixture ofl-benzyl-1H-1,2,4-triazol-5-amine 2,2,2-trifluoroacetate (mixture of isomers) (292 mg, 1.01 mmol, Eq: 1.24), 1-bromo-3,5-dichlorobenzene (185 mg, 819 μmol, Eq: 1.00), sodium tert-butoxide (341 mg, 3.55 mmol, Eq: 4.33), tris(dibenzylideneacetone)dipalladium(0) (18.7 mg, 20.5 μmol, eq: 0.025) and 2-di-tert-butylphosphino-2′,4′,6′-triisopropylbiphenyl (34.8 mg, 81.9 μmol, Eq: 0.10) in a 2-5 mL microwave vessel. Sealed the vessel. Evacuated the system and re-charged with nitrogen (2×). Added toluene and heated in an oil bath at 110° C. overnight.
The reaction mixture was diluted with EtOAc, washed with water and brine. Dried (Na2SO4) and concentrated over celite. The crude material was purified by flash chromatography (silica gel, SF40-80 g, 25% to 50% EtOAc in hexanes) to give 2 major products. The more polar fraction was desired product 116 mg, which was triturated with methanol, filtered and rinsed with ether to give 42 mg (16%) of desired product as a white solid. The less polar fraction was determined to be isomeric product, (2-Benzyl-2H-[1,2,4]triazol-3-yl)-(3,5-dichloro-phenyl)-amine.
MS m/z 319 [M+H]
A mixture ofl-benzyl-1H-1,2,4-triazol-5-amine 2,2,2-trifluoroacetate (mixture of isomers) (292 mg, 1.01 mmol, Eq: 1.24), 1-bromo-3,5-dichlorobenzene (185 mg, 819 μmol, Eq: 1.00), sodium tert-butoxide (341 mg, 3.55 mmol, Eq: 4.33), tris(dibenzylideneacetone)dipalladium(0) (18.7 mg, 20.5 μmol, eq: 0.025) and 2-di-tert-butylphosphino-2′,4′,6′-triisopropylbiphenyl (34.8 mg, 81.9 μmol, Eq: 0.10) in a 2-5 mL microwave vessel. Sealed the vessel. Evacuated the system and re-charged with nitrogen (2×). Added toluene and heated in an oil bath at 110° C. overnight. The reaction mixture was diluted with EtOAc, washed with water and brine. Dried (Na2SO4) and concentrated over celite. The crude material was purified by flash chromatography (silica gel, SF40-80 g, 25% to 50% EtOAc in hexanes) to give 2 major products. The less polar fraction was desired product, 42 mg (16%) as a colorless oil (2-Benzyl-2H-[1,2,4]triazol-3-yl)-(3,5-dichloro-phenyl)-amine. The more polar fraction was isomeric product, (1-Benzyl-1H-[1,2,4]triazol-3-yl)-(3,5-dichloro-phenyl)-amine.
MS m/z 319 [M+H]
In a 200 mL round-bottomed flask, 1-benzyl-N-(3,5-dichlorophenyl)-1H-1,2,4-triazol-3-amine Compound 23 (0.208 g, 652 μmol, Eq: 1.00) was combined with MeOH (30 mL) to give a light yellow suspension. Hydrogen chloride (in dioxane) (6.5 mL, 26.0 mmol, Eq: 39.9) was added. Solid quickly went into solution, resulting in a light yellow solution.
Evacuated the system and recharged with nitrogen (2×). Palladium on carbon (340 mg, 319 μmol, Eq: 0.490) was added. Evacuated the system and recharged with nitrogen and then evacuated and recharged with hydrogen(2×). Stirred overnight at room temperature under hydrogen atmosphere. LCMS indicated imcomplete conversion after 16 hr. Added additional catalyst (0.215 g) and recharged with hydrogen and stirred overnight. LCMS after 38 hr total reaction time indicated nearly complete conversion. Filtered the entire reaction mixture through a bed of celite and washed with methanol. Concentrated the filtrate. Partitioned the residue between EtOAc and water and neutralized with saturated NaHCO3. The organic phase was washed with brine and concentrated over celite. The crude material was purified by flash chromatography (silica gel, SF25-40 g, 50% to 100% EtOAc in hexanes) to give 55 mg of desired product with small impurity. The chromatographed material was then triturated with ether/hexane. The solid was collected and dried under house vacuum with heat to give 43 mg (29%) of desired product as an off-white solid.
MS m/z 229 [M+H]
In a 50 mL round-bottomed flask, 5-bromo-1H-1,2,4-triazole (0.685 g, 4.63 mmol, Eq: 1.00) was combined with 0.5 M sodium methoxide in MeOH (9.26 ml, 4.63 mmol, Eq: 1.00) give a light yellow solution. The solution was stirred for 5-10 minutes and was then concentrated. The solid residue was dissolved in DMF (5.00 ml) and (bromomethyl)benzene (792 mg, 550 μl, 4.63 mmol, Eq: 1.00) was added. Stirred at room temperature under a nitrogen atmosphere for 6 hours. The reaction was diluted with EtOAc and washed with water (2×) and brine (1×). Each aqueous phase was backwashed with EtOAc. The two organic extracts were combined and concentrated over celite. The crude material was purified by flash chromatography (silica gel, SF25-40 g, 25% to 50% EtOAc in hexanes) to give 2 major components. The less polar fraction was determined to be desired product, 1-benzyl-5-bromo-1H-1,2,4-triazole (0.197 g, 827 μmol, 17.9% yield). The more polar fraction was determined to be isomer, 1-benzyl-3-bromo-1H-1,2,4-triazole (0.612 g, 2.57 mmol, 55.5% yield), described in the procedures for Compound 27.
In a 10-20 mL microwave vial, 1-benzyl-5-bromo-1H-1,2,4-triazole (170 mg, 714 μmol, Eq: 1.00), 3,5-dichloro-4-fluoroaniline (154 mg, 857 μmol, Eq: 1.20), sodium tert-butoxide (230 mg, 2.39 mmol, Eq: 3.35), tris(dibenzylideneacetone)dipalladium (0) (16.8 mg, 18.3 μmol, Eq: 0.0257) and di-tert-butyl(2′,4′,6′-triisopropylbiphenyl-2-yl)phosphine (32.9 mg, 77.5 μmol, Eq: 0.109) were combined. The system was evacuated and recharged with nitrogen. Toluene (2.0 ml) was added to give a dark red mixture. The reaction mixture was heated to 110° C. and stirred for 18 h. Cooled to room temperature. Partitioned the reaction mixture between EtOAc and water. The organic phase was removed, washed with brine and concentrated over celite. The crude material was purified by flash chromatography (silica gel, SF15-24 g, 20% to 50% EtOAc in hexanes over 9 minutes) to give a brown oily residue (47 mg) containing desired product and an impurity. This material was then re-purified by flash chromatography (silica gel, Sunfire Prep C18 OBD [5 uM; 30×100 mm], 40% to 95% acetonitrile in water (each containing 0.1% formic acid)) in multiple runs. Product containing fractions from each run were combined, concentrated and then freeze-dried. The freeze-dried material was dissolved in EtOAc and extracted with saturated sodium bicarbonate (to insure neutralization) and then water. The organic phase was dried (Na2SO4) and concentrated. The residue was dissolved in acetonitrile-water and freeze-dried to give 37 mg (15%) of desired product as an off-white solid.
MS m/z 337 [M+H]
In a 50 mL round-bottomed flask, 5-bromo-1H-1,2,4-triazole (0.685 g, 4.63 mmol, Eq: 1.00) was combined with 0.5 M sodium methoxide in MeOH (9.26 ml, 4.63 mmol, Eq: 1.00) give a light yellow solution. The solution was stirred for 5-10 minutes and was then concentrated. The solid residue was dissolved in DMF (5.00 ml) and (bromomethyl)benzene (792 mg, 550 μl, 4.63 mmol, Eq: 1.00) was added. Stirred at room temperature under a nitrogen atmosphere for 6 hours. The reaction was diluted with EtOAc and washed with water (2×) and brine (1×). Each aqueous phase was backwashed with EtOAc. The two organic extracts were combined and concentrated over celite. The crude material was purified by flash chromatography (silica gel, SF25-40 g, 25% to 50% EtOAc in hexanes) to give 2 major components. The more polar fraction was determined to be desired product, 1-benzyl-3-bromo-1H-1,2,4-triazole (0.612 g, 2.57 mmol, 55.5% yield). The less polar fractions were determined to be isomeric product, 1-benzyl-5-bromo-1H-1,2,4-triazole, as described in the procedure for Compound 26.
In a 10-20 mL microwave vial, 1-benzyl-3-bromo-1H-1,2,4-triazole (217 mg, 911 μmol, Eq: 1.00), 3,5-dichloro-4-fluoroaniline (228 mg, 1.27 mmol, Eq: 1.39), sodium tert-butoxide (308.6 mg, 3.21 mmol, Eq: 3.52), tris(dibenzylideneacetone)dipalladium (0) (21.1 mg, 23.0 μmol, Eq: 0.0253) and di-tert-butyl(2′,4′,6′-triisopropylbiphenyl-2-yl)phosphine (39.4 mg, 92.8 μmol, Eq: 0.102) were combined. The system was evacuated and recharged with nitrogen (2×). Toluene (2.6 ml) was then added. The reaction mixture was heated to 110° C. and stirred for 19 h. The reaction mixture was partitioned between EtOAc and water. The organic phase was separated, washed with brine and concentrated over celite. The crude material was purified by flash chromatography (silica gel, SF25-40 g, 25% to 50% EtOAc in hexanes) and the fractions were combined, concentrated and triturated to give 55 mg (18%) of desired product. A second crop of solid precipitated out of the filtrate to give 28 mg (9%) of desired product with impurities. The mother liquors were combined and purified by flash chromatography in multiple runs (reverse phase, Sunfire Prep C18 OBD [5 uM; 30×100 mm], 50% to 95% acetonitrile in water (each containing 0.1% TFA)). Product-containing fractions from each run were combined and concentrated. The reisdue was dissolved in EtOAc and washed with saturated NaHCO3 to ensure neutralization of the TFA. The organic phase was dried and concentrated. The solid residue was triturated with ether and the solid was filtered and dried to give 21 mg (7%) of desired product.
MS m/z 337 [M+H]
A 100 mL round-bottomed flask was charged with 1-benzyl-N-(3,5-dichloro-4-fluorophenyl)-1H-1,2,4-triazol-3-amine Compound 27 (113 mg, 335 μmol, Eq: 1.00). Methanol (15.0 ml) was added, resulting in a suspension. The hydrogen chloride (in dioxane) (3.35 ml, 13.4 mmol, Eq: 40) solution was added and the solid rapidly went into solution. This solution was evacuated and recharged with nitrogen (2×). Palladium on carbon (187 mg, 176 μmol, Eq: 0.524) was added. The mixture was evacuated and recharged with nitrogen and then with hydrogen. The mixture was stirred overnight under atmospheric hydrogen. LCMS indicated incomplete conversion. Additional catalyst (83 mg) was added. The mixture was evacuated and recharged with hydrogen and stirred overnight. LCMS indicated further conversion to product, but imcomplete reaction. Recharged with hydrogen and stirred for another 12 hours. LCMS showed the reaction to be complete. Filtered the entire reaction mixture through a bed of celite. The filtrate was concentrated. The residue was partitioned between EtOAc and water and then neutralized with saturated sodium bicarbonate. The organic phase was removed, washed with water, dried (Na2SO4) and concentrated over celite. The crude material was purified by flash chromatography (silica gel, SF15-24 g, 50% to 100% EtOAc in hexanes). The purified product was dissolved in acetonitrile-water and freeze-dried to give 26 mg (32%) of desired product as an off-white product.
MS m/z 247 [M+H]
In a 25 mL round-bottomed flask, 5-bromo-1H-1,2,4-triazole (0.207 g, 1.4 mmol, Eq: 1.00) was combined with sodium methoxide in MeOH (2.8 mL, 1.4 mmol, Eq: 1.00) give a light yellow solution. Stirred for 5 minutes and concentrated. The solid residue was dissolved in DMF (1.51 mL) and (bromomethyl)benzene (239 mg, 166 μL, 1.4 mmol, Eq: 1.00) was added. Stirred at room temperature under a nitrogen atmosphere. After ˜10 hours, diluted the reaction with EtOAc. This organic phase was washed with water (2×) and brine (1×) and then concentrated over celite. The crude material was purified by flash chromatography (silica gel, SF15-24 g, 25% to 50% EtOAc in hexanes) to give 209 mg (63%) of desired product.
In a 10-20 mL microwave vial, 1-benzyl-3-bromo-1H-1,2,4-triazole (145.7 mg, 612 μmol, Eq: 1.00), tert-butyl 3-aminophenylcarbamate (157.8 mg, 758 μmol, Eq: 1.24), sodium tert-butoxide (176.5 mg, 1.84 mmol, Eq: 3.00), tris(dibenzylideneacetone)dipalladium(0) (14.0 mg, 15.3 μmol, Eq: 0.0250) and 2-di-tert-butylphosphino-2′,4′,6′-triisopropylbiphenyl (26.7 mg, 62.8 μmol, Eq: 0.103) were combined with toluene (1.75 ml) to give a red mixture. The mixture was heated in an oil bath at 110° C. and quickly went to a brown color and thickened. After 15 hours, the reaction was cooled to room temperature. The reaction mixture was partitioned between EtOAc and water. Separated the layers. Washed the aqueous phase with additional EtOAc (2×). Each organic phase was backwashed with brine (1×). The organic phases were combined and concentrated over celite. The crude material was purified by flash chromatography (silica gel, SF25-40 g, 30% to 100% EtOAc in hexanes over 12 minutes) to give 48 mg of desired product.
In a 10 mL pear-shaped flask, tert-butyl 3-(1-benzyl-1H-1,2,4-triazol-3-ylamino)phenylcarbamate (75.6 mg, 207 μmol, Eq: 1.00) was combined with dioxane (2.0 ml) to give a light brown solution. Hydrogen chloride (in dioxane) (2.0 ml, 8.00 mmol, Eq: 38.7) was added and solution was stirred at room temperature under a nitrogen atmosphere. The reaction was initially a clear solution and then solid began to drop out of solution. After 4 hours, the reaction looked to be nearly complete and was concentrated under a stream of nitrogen. The residue was partitioned between EtOAc and water and then neutralized with saturated sodium bicarbonate. The aqueous phase was removed and extracted with EtOAc (2×). Each organic phase was washed with brine. The three organic phases were then combined and concentrated over celite. The crude material was purified by flash chromatography (silica gel, SF15-12 g, 30% to 100% EtOAc in hexanes over 7 minutes) to give 42 mg of desired product, containing an impurity. This material was purified a second time by reverse phase chromatography. The product-containing fractions were combined, concentrated and freeze-dried. The residue was dissolved in EtOAc, washed with sodium bicarbonate and then brine, dried (Na2SO4) and concentrated. The residue was then dissolved in acetonitrile-water and freeze-dried, giving 10 mg (18%) of desired product as an off-white solid.
MS m/z 266 [M+H]
In a 10-20 mL microwave vial, 5-bromo-1H-1,2,4-triazole (0.734 g, 4.96 mmol, Eq: 1.00), 1-(chloromethyl)-4-methoxybenzene (1.15 g, 1.00 mL, 7.37 mmol, Eq: 1.49) and N,N-diisopropylethylamine (1.29 g, 1.74 mL, 9.96 mmol, Eq: 2.01) were combined with acetonitrile (14.0 mL) to give a colorless solution. Potassium iodide (424 mg, 2.55 mmol, Eq: 0.515) was added. The reaction was heated in an oil bath at 80° C. The reaction went to a yellow color and then darkened to a brown color. Additional solid looked to precipitate out of solution. Cooled to room temperature after ˜3 hours. Purification by chromatography gave 575 mg (43%) of desired product, as the more polar component confirmed by NOE. The less polar component (267 mg, 20%) was determined to be the regioisomer, 5-bromo-1-(4-methoxy-benzyl)-1H-[1,2,4]triazole.
In a 2-5 mL microwave vial 2,6-dichloropyridin-4-amine (121 mg, 742 μmol, Eq: 1.24), sodium tert-butoxide (172 mg, 1.79 mmol, Eq: 3.00), tris(dibenzylideneacetone)dipalladium (0) (32.4 mg, 35.4 μmol, Eq: 0.0593) and di-tert-butyl(2′,4′,6′-triisopropylbiphenyl-2-yl)phosphine (62.2 mg, 146 μmol, Eq: 0.245) were combined. The system was evacuated and recharged with nitrogen (2×). A solution of 3-bromo-1-(4-methoxybenzyl)-1H-1,2,4-triazole (160 mg, 597 μmol, Eq: 1.00) in Toluene (1.66 ml) was then added. The mixture was stirred at room temperature for 5 minutes and then heated at 110° C. overnight. The reaction mixture was cooled to room temperature and partitioned between EtOAc and water. The organic phase was washed with brine, dried over Na2SO4 and concentrated in vacuo over celite. The crude material was purified by flash chromatography (silica gel, SF15-12 g, 20% to 100% EtOAc in hexanes) to give impure fractions. The product-containing fractions were combined and concentrated. The residue was dissolved in methylene chloride-methanol and the solution allowed to sit at room temperature open to the air. As solvent slowly evaporated solid started to precipitate out. The solid was collected and show to be the desired product contaminated with a small amount of impurity. The mother liquor was concentrated over celite and purified a second time by flash chromatography (silica gel, SF15-24 g, 2% to 5% MeOH in DCM) to give 150 mg (72%) of desired product as a light yellow solid.
In a 10-20 mL microwave vial, 2,6-dichloro-N-(1-(4-methoxybenzyl)-1H-1,2,4-triazol-3-yl)pyridin-4-amine (145 mg, 414 μmol, Eq: 1.00) was combined with TFA (8 mL) to give a yellow solution. The reaction mixture was heated in the microwave at 120° C. for 1 h. The reaction was concentrated. The residue was taken up in EtOAc and washed with saturated NaHCO3 and brine, dried (Na2SO4) and concentrated over celite. The crude material was first chromatographed by normal phase flash chromatography and then by reverse phase HPLC to give 2,6-dichloro-N-(1H-1,2,4-triazol-3-yl)pyridin-4-amine (50 mg, 217 μmol, 52.5% yield). The material was combined with another batch of product, prepared similarly. The combined lot was taken up in EtOAc and washed with bicarbonate (to ensure removal of TFA) and then water. The organic phase was dried (Na2SO4) and concentrated. The residue was dissolved in hot EtOAc and cooled to near room temperature. While still warm the solution was filtered through a cotton plug to remove any insoluble particles. The filtrate continued to cool down and solid began to crystallize out of solution. The mixture was concentrated to a small volume and diluted with hexanes. The solid was collected by filtration and washed with EtOAc-heaxane (˜1:1). Dried under vacuum at 80° C. overnight to give 58 mg (60%) of desired product as a white solid. MS m/z 230, 232 [M+H]
In a 500 mL round-bottomed flask, 5-bromo-3-nitro-1H-1,2,4-triazole (4.00 g, 20.7 mmol, Eq: 1.00), 1-(chloromethyl)-4-methoxybenzene (3.25 g, 2.81 ml, 20.7 mmol, Eq: 1.00) and N,N-diisopropylethylamine (5.36 g, 7.24 ml, 41.5 mmol, Eq: 2.00) were combined with acetonitrile (207 ml) to give a yellow solution. Added potassium iodide (1.72 g, 10.4 mmol, Eq: 0.50). The reaction mixture was heated to 85° C. and stirred for 3 h. Cooled to room temperature and concentrated. The residue was taken up in EtOAc (200 mL) and washed with water (100 mL) and brine (100 mL). The organic layer were dried over Na2SO4 and concentrated in vacuo over celite. The crude material was purified by flash chromatography (silica gel, Silicycle 330 g, 0% to 100% EtOAc in hexanes) to give 1.61 g (25%) of desired isomer as a less polar fraction, and 3.26 g (50%) of isomeric product (5-Bromo-1-(4-methoxy-benzyl)-3-nitro-1H-[1,2,4]triazole) as a more polar fraction.
In a 2-5 mL microwave vial, 3-bromo-1-(4-methoxybenzyl)-5-nitro-1H-1,2,4-triazole (199.1 mg, 636 μmol, Eq: 1.00) and morpholine (111 mg, 110 μl, 1.27 mmol, Eq: 2.00) were combined with THF (1.2 ml) to give a yellow suspension. Heated in an oil bath at 85° C. for 2-3 hours. Cooled to room temperature. Diluted the reaction solution with EtOAc. Washed with water (1×) and brine (3×). The crude material was purified by flash chromatography (silica gel, SF15-12 g, 20% to 100% EtOAc in hexanes) to give 120 mg (53%) of desired product.
In a 2-5 mL microwave vial, 4-(3-bromo-1-(4-methoxybenzyl)-1H-1,2,4-triazol-5-yl)morpholine (133.5 mg, 378 μmol, Eq: 1.00), 3,5-dichloro-4-fluoroaniline (81.6 mg, 454 μmol, Eq: 1.20), sodium tert-butoxide (109 mg, 1.13 mmol, Eq: 3.00), tris(dibenzylideneacetone)dipalladium (0) (20.8 mg, 22.7 μmol, Eq: 0.06) and di-tert-butyl(2′,4′,6′-triisopropylbiphenyl-2-yl)phosphine (38.5 mg, 90.7 μmol, Eq: 0.24) were combined. The system was evacuated and recharged with nitrogen (2×). Toluene (1.08 ml) was then added. The mixture was stirred at room temperature for 5 minutes and then heated at 85° C. for 15 h. After cooling to room temperature, the reaction mixture was partitioned between EtOAc and water. The organic layer was removed, washed with brine, dried (Na2SO4) and concentrated over celite. The crude material was purified by flash chromatography (silica gel, SF15-24 g, 20% to 100% EtOAc in hexanes) to gove 90 mg (53%) of desired product.
In a 10 mL round-bottomed flask, N-(3,5-dichloro-4-fluorophenyl)-1-(4-methoxybenzyl)-5-morpholino-1H-1,2,4-triazol-3-amine (85.1 mg, 188 μmol, Eq: 1.00) was combined with TFA (1.50 ml) to give a light brown solution. The reaction mixture was heated to 65° C. and stirred for 2 h. After cooling to room temperature, the crude reaction mixture was purified directly by flash chromatography (silica gel, Sunfire Prep C18 OBD [5 uM; 30×100 mm], 5% to 95% acetonitrile in water (each containing 0.1% TFA)). The product-containing fractions were combined, concentrated and freeze-dried overnight. The freeze-dried material was dissolved in EtOAc and washed with sat'd NaHCO3 (to ensure formation of the free base) and then brine. The organic phase was dried (Na2SO4) and concentrated to a film. Ether was added and the film on the walls of the flask quickly solidified. The solid was collected and dried in vacuo at 80° C. for 10 hours. The NMR showed an entire mole of ether to still be present. The solid was re-dissolved in acetonitrile-water and freeze-dried to give 24 mg (38%) of desired product as an off-white solid.
MS m/z 332, 334 [M+H]
In a 25 mL round-bottomed flask, ammonium thiocyanate (587 mg, 7.72 mmol, Eq: 1.25) was combined with acetone (5 mL) to give a colorless solution. Isobutyryl chloride (822 mg, 815 μL, 7.72 mmol, Eq: 1.25) was added. White solid immediately began to drop out of solution and mixture became very thick. Stirred at room temperature under nitrogen for 1 hour.
Filtered to remove the solid and washed with acetone (2 mL). The yellow filtrate was added to a colorless solution of 3,5-dichloroaniline (1.00 g, 6.17 mmol, Eq: 1.00) in acetone (3 mL). The resulting solution was light yellow in color and slightly cloudy. Heated to reflux for 6.5 hours. Cooled to room temperature and stirred overnight. Diluted the reaction mixture with EtOAc and then concentrated over celite. The crude material was purified by flash chromatography (silica gel, SF25-40 g, 0% to 20% EtOAc in hexanes) to give impure product, which was further purified using a 13% EtOAc in hexanes isocratic system. Pure product fractions from each chromatography were combined and concentrated to 1.53 g (85%) of desired product as a white solid.
In a 25 mL round-bottomed flask, N-(3,5-dichlorophenylcarbamothioyl)isobutyramide (0.512 g, 1.76 mmol, Eq: 1.00), diiodomethane (1.41 g, 426 μL, 5.27 mmol, Eq: 3.00) and triethylamine (534 mg, 735 μL, 5.27 mmol, Eq: 3.00) were combined with acetone (7.5 mL) to give a colorless solution. Heated to reflux and stirred overnight under a N2 atmosphere. The reaction was cooled to room temperature and filtered to remove the insoluble Et3N.HI salt. The filtrate was concentrated and purified by flash chromatography (silica gel, SF25-40 g, 10% EtOAc in hexanes) to give 193 mg (36%) of desired product.
In a 10-20 mL microwave vial, (Z)-1-(2-(3,5-dichlorophenylimino)-1,3-thiazetidin-3-yl)-2-methylpropan-1-one (190 mg, 627 μmol, Eq: 1.00) was combined with Acetonitrile (5.0 mL) to give a colorless suspension. Capped the vial and charged with nitrogen. Added hydrazine (204 mg, 200 μL, 6.37 mmol, Eq: 10.2) and stirred at room temperature for 5 minutes. Most of the solid went into solution. Heated in an oil bath at 85-90° C. overnight. The reaction mixture was concentrated. The crude material was initially purified by flash chromatography (silica gel, SF15-24 g, MeOH in DCM) but the purification was unsuccessful. A second chromatograpy was then performed by HPLC (reverse phase, Sunfire Prep C18 OBD [5 uM; 30×100 mm], 5% to 95% acetonitrile in water (each containing 0.1% TFA)) in multiple runs. The product-containing fractions were combined and freeze-dried. The freeze-dried material was dissolved and washed with saturated NaHCO3 to neutralize any TFA and ensure formation of the free base. The organic phase was dried (Na2SO4) and concentrated. The residue was triturated, the solid collected and dried to give 69 mg (40%) of desired product as a white solid.
MS m/z 271, 273 [M+H]
In a 25 mL round-bottomed flask, ammonium thiocyanate (587 mg, 7.72 mmol, Eq: 1.25) was combined with acetone (5 mL) to give a colorless solution. Added 3-methylbutanoyl chloride (930 mg, 940 μL, 7.71 mmol, Eq: 1.25). White solid immediately began to precipitate out of solution. After 1 hour, the suspension was filtered to remove the solid and washed with acetone (2 mL). Added the filtrate to a solution of 3,5-dichloroaniline (1.0 g, 6.17 mmol, Eq: 1.00) in acetone (5 mL). Heated to reflux (oil bath temperture was 70° C.) for 6 hours. Cooled to room temperature and stirred overnight. The reaction mixture was concentrated and purified by flash chromatography (silica gel, SF25-40 g, 10% EtOAc in hexanes) to give impure fractions. Further purification flash chromatography (40 g; 12% EtOAc in hexanes) gave 1.392 g (74%) of desired product as a white solid.
In a 10-20 mL microwave vial, N-(3,5-dichlorophenylcarbamothioyl)-3-methylbutanamide (0.500 g, 1.64 mmol, Eq: 1.00), diiodomethane (1.31 g, 395 μl, 4.9 mmol, Eq: 2.99) and triethylamine (497 mg, 685 μl, 4.91 mmol, Eq: 3.00) were combined with Acetone (7.1 ml) to give a colorless solution. The reaction mixture was heated to 65-70° C. and stirred for 18 h. The reaction was cooled to room temperature and filtered to remove the Et3N.HI. The filtrate was concentrated and purified by flash chromatography (silica gel, SF25-40 g, 15% EtOAc in hexanes) to give 235 mg (45%) of desired product as a white solid.
In a 10-20 mL microwave vial, (Z)-1-(2-(3,5-dichlorophenylimino)-1,3-thiazetidin-3-yl)-3-methylbutan-1-one (0.324 g, 1.02 mmol, Eq: 1.00) was combined with acetonitrile (8.0 mL) to give a suspension. Added hydrazine (327 mg, 320 μL, 10.2 mmol, Eq: 9.98). Capped the vial and charged the vessel with nitrogen. The solid went into solution. The reaction mixture was heated to 85-90° C. and stirred for 18 h. The color went from colorless to green and back to colorless. Upon cooling to room temperature, a white solid dropped out of solution. The solid was collected, washed with acetonitrile, and dried to give 228 mg (78%) of desired product as a white solid.
MS m/z 285, 287 [M+H]
In a 25 mL round-bottomed flask, ammonium thiocyanate (587 mg, 7.72 mmol, Eq: 1.25) was combined with acetone (5 mL) to give a colorless solution. 2-Phenylacetyl chloride (1.17 g, 1.0 ml, 7.56 mmol, Eq: 1.23) was added. A white solid immediately began to precipitate out of solution. The reaction mixture was stirred at room temperature for 50 minutes. The suspension was filtered to remove the solid and washed the solid with 3 mL acetone. The filtrate and washes were added to a solution of 3,5-dichloroaniline (1.00 g, 6.17 mmol, Eq: 1.00) in acetone (2 mL). Capped the vial. The reaction mixture was heated at 70° C. for 6 h, then stirred at room temperature overnight. The reaction mixture was concentrated and chromatographed to give impure fractions. Further purfication by flash chromatography gave 1.608 g (77%) of desired product as a light yellow solid.
In a 10-20 mL microwave vial, N-(3,5-dichlorophenylcarbamothioyl)-2-phenylacetamide (0.500 g, 1.47 mmol, Eq: 1.00) and diiodomethane (1.2 g, 360 μL, 4.46 mmol, Eq: 3.03) were combined with Acetone (6.4 mL) to give a colorless solution. Added triethylamine (450 mg, 620 μL, 4.45 mmol, Eq: 3.02). The orange reaction mixture was heated to 70° C. and stirred for 16 h. Solution lightened to a light yellow color and then solid began to precipitate out of solution. Cooled to room temperature and filtered to remove the Et3N. HI salt. The filtrate was concentrated over and purified by flash chromatography. The product was concentrated and triturated with ether. The resulting solid was filtered and air-dried under suction to give 154 mg (30%) of desired product as a white solid.
In a 10-20 mL microwave vial, (Z)-1-(2-(3,5-dichlorophenylimino)-1,3-thiazetidin-3-yl)-2-phenylethanone (152 mg, 433 μmol, Eq: 1.00) and hydrazine (139 mg, 136 μL, 4.33 mmol, Eq: 10.0) were combined with acetonitrile (3.3 mL). The mixture was heated in an oil bath at 90° C. overnight. The resulting suspension was cooled to room temperature and filtered The solid and the mother liquor were then purified by reverse phase HPLC in multiple runs. The product-containing fractions from each run were combined and freeze-dried.
The resulting solid, most likely the TFA salt was dissolved in EtOAc and washed with aqueous NaHCO3 to neutralize the TFA. The organic phase was then washed with water (3×) to neutral pH, dried (Na2SO4) and concentrated. The solid residue was triturated with acetontrile. The solid was collected and dried in a vacuum oven at 100° C. for 4 hours to give 84 mg (60%) of desired product as a white solid.
MS m/z 319, 321 [M+H]
Determination of compounds HCV GT1b and GT1a entry inhibitory activity using the pseudotyped HCV particle (HCVpp) reporter assay.
Mammalian expression plasmids for the generation of pseudotyped virus particles.
Plasmids expressing HCV El and E2 envelope proteins of GT1a H77 strain (Proc Natl Acad Sci USA 1997 94:8738-43) or GT1b Con1 strain (Science 1999 285:110-3) were constructed by cloning the nucleic acids encoding the last 60 amino acids of HCV core protein and all of the HCV E1 and E2 proteins into pcDNA3.1(+) vector. Plasmid pVSV-G expressing the glycoprotein G of the vesicular stomatitis virus (VSV G) is from Clontech (cat #631530). The HIV packaging construct expressing the firefly luciferase reporter gene was modified based on the envelope defective pNL.4.3.Luc-R−.E− vector (Virology 1995 206:935-44) by further deleting part of the HIV envelope protein.
Generation of pseudotyped virus particles in transiently transfected HEK-293T cells.
Pseudotyped HCV GT1a and GT1b particles (HCVpp) and the pseudotyped VSV G particles (VSVpp) were generated from transiently transfected HEK-293T cells (ATCC cat #CRL-573). For generating HCVpp, the HEK-293T cells were transfected with equal amounts of plasmids expressing the HCV envelope proteins and the HIV packaging genome by using polyethylenimine (Polysciences cat #23966) as transfection reagent. For generating VSVpp, the HEK-293T cells were transfected with equal amounts of plasmids expressing VSV G and the HIV packaging genome by using polyethylenimine. 24 hours after the transfection, the cell culture medium containing the transfection mixture was replaced with fresh Dulbecco's Modified Eagle Medium (DMEM-Glutamax™-I; Invitrogen cat #10569-010) supplemented with 10% Fetal Bovine Serum (Invitrogen cat #10082-147) and 2 mM L-glutamine (Invitrogen cat #25030-081). The supernatant was collected 48 hours after the transfection and filtered through a sterile 0.45 μm filter. Aliquots of the supernatant was frozen and stored at −80° C. until use.
Huh7-high CD81 cells with high CD81 expression level were enriched by flow cytometry sorting using FITC-labeled CD81 antibody JS-81 (BD Biosciences cat #561956) to allow more efficient HCV entry. The Huh7-high CD81 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM-Glutamax™-I; Invitrogen cat #10569-010). The medium was supplemented with 10% Fetal Bovine Serum (Invitrogen cat #10082-147) and 1% penicillin/streptomycin (Invitrogen cat #15070-063). Cells were maintained at 37° C. in a humidified 5% CO2 atmosphere.
Determination of compound HCVpp entry inhibitory activity in Huh7-high CD81 cells.
Huh7-high CD81 cells were plated at a cell density of 8000 cells per well in 96 well plates (Perkin Elmer, cat #6005660). Cells were plated in 100 μl of Dulbecco's Modified Eagle Medium (DMEM-Glutamax™-I, Invitrogen Cat #10569-010) supplemented with 10% Fetal Bovine Serum (Invitrogen Cat #10082-147) and 1% penicillin/streptomycin (Invitrogen cat #15070-063). Cells were allowed to equilibrate for 24 hours at 37° C. and 5% CO2 at which time compounds and pseudotyped viruses were added. On the day of the assay, HCVpp aliquots were thawed in 37° C. water bath and kept at 4° C. until use. Compounds (or medium as a control) were diluted in 3 fold dilution series in DMEM-Glutamax™-I with 2% DMSO and 2% penicillin/streptomycin. The 100 μl plating medium in each culture well was removed followed by the addition of 50 μl compound dilutions and 50 μl thawed HCVpp. Firefly luciferase reporter signal was read 72 hours after the addition of compounds and HCVpp using the Steady-Glo luciferase Assay System (Promega, cat #E2520) following the manufacturer's instruction. EC50 values were defined as the compound concentration at which a 50% reduction in the levels of firefly luciferase reporter was observed as compared to control samples in the absence of compound and was determined by non-linear fitting of compound dose-response data.
Determination of compound selectivity in Huh7-high CD81 cells.
Huh7 hCD81 cell assay plates and compound dilutions were set up in the same format as in the HCVpp assay. 24 hours after cell plating, thawed VSVpp was diluted by 800 fold in DMEM-Glutamax™-I supplemented with 10% fetal bovine serum. After removal of the cell plating medium from the culture wells, 50 μl compound dilutions and 50 μl diluted VSVpp were added to the wells. Firefly luciferase reporter signal was read 72 hours after the addition of compounds and VSVpp using the Steady-Glo luciferase Assay System (Promega, cat #E2520). EC50 values were defined as the compound concentration at which a 50% reduction in the levels of firefly luciferase reporter was observed as compared to control samples in the absence of compound and was determined by non-linear fitting of compound dose-response data. The EC50 was approximated if maximum percentage inhibition was less than 90% and more than 70%. Representative assay data can be found in Table II below:
The foregoing invention has been described in some detail by way of illustration and example, for purposes of clarity and understanding. It will be obvious to one of skill in the art that changes and modifications may be practiced within the scope of the appended claims.
Therefore, it is to be understood that the above description is intended to be illustrative and not restrictive. The scope of the invention should, therefore, be determined not with reference to the above description, but should instead be determined with reference to the following appended claims, along with the full scope of equivalents to which such claims are entitled.
All patents, patent applications and publications cited in this application are hereby incorporated by reference in their entirety for all purposes to the same extent as if each individual patent, patent application or publication were so individually denoted.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2014/054016 | 3/3/2014 | WO | 00 |
| Number | Date | Country | |
|---|---|---|---|
| 61772936 | Mar 2013 | US |
