Antiviral methods

Description

BACKGROUND OF THE INVENTION
The present invention relates to novel forms and multimeric configurations of intercellular adhesion molecule (ICAM), including both full-length and truncated forms of these proteins, that effectively bind to human rhinovirus and can effectively reduce HRV infectivity, and to methods of making and using same.
Full-length ICAM, also known as human rhinovirus receptor (HRR), is termed transmembrane ICAM (tmICAM-1); non-transmembrane ICAM forms, also known as truncated ICAM (tICAM), are less than full length. When in a multimeric configuration, preferably as dimers, these proteins display enhanced binding of human rhinovirus (HRV) and are able to reduce HRV infectivity. In addition, these multimerized proteins may also be used to reduce infectivity of other viruses that are known to bind to the `major` group human rhinovirus receptor (HRR), such as Coxsackie A virus, and may also be used to block transmembrane intercellular adhesion molecule (tmICAM) interaction with lymphocyte function-associated antigen-1 (LFA-1), which is critical to many cell adhesion processes involved in the immunological response. Lastly, these multimerized proteins may be used to study the ICAM-1/HRV interaction especially with respect to designing other drugs directed at affecting this interaction.
Human rhinoviruses are the major causative agent of the common cold. They belong to the picornavirus family and can be classified based on the host cell receptor to which they bind. Tomassini, et al., J. Virol., 58: 290 (1986) reported the isolation of a receptor protein involved in the cell attachment of human rhinovirus. Approximately 90% of the more than 115 serotypes of rhinoviruses, as well as several types of Coxsackie A virus, bind to a single common receptor termed the "major" human rhinovirus receptor (HRR); the remaining 10% bind to one or more other cell receptors.
Recently, Greve, J. et al., Cell, 56:839 (1989), co-authored by the co-inventors herein, identified the major HRR as a glycoprotein with an apparent molecular mass of 95,000 daltons and having an amino acid sequence essentially identical to that deduced from the nucleotide sequence of a previously described cell surface protein named intercellular adhesion molecule (ICAM-1) [Simmons, D. et al., Nature, 331:624 (1988); Staunton, et al., Cell, 52:925-933 (1988)]. Subsequently, Staunton, D. E., et al., Cell, 56:849 (1989), confirmed that ICAM-1 is the major surface receptor for HRV. See also, Staunton, et al., Cell, 61:243-254 (1990).
ICAM-1 is an integral membrane (numbered acording to Staunton et al., 1988) protein 505 amino acids long [SEQ ID NO:19] and has: i) five immunoglobulin-like extracellular domains at the amino-terminal end (amino acid residues 1-453), ii) a hydrophobic transmembrane domain (454-477), and iii) a short cytoplasmic domain at the carboxy-terminal end (478-505). See FIG. 1. ICAM-1 is a member of the immunoglobulin supergene family and functions as a ligand for the leukocyte molecule, lymphocyte function associated molecule-1 (LFA-1), a member of the integrin family. Heterotypic binding of LFA-1 to ICAM-1 mediates cellular adhesion of diverse cell types and is important in a broad range of immune interactions; induction of ICAM-1 expression by cytokines during the inflammatory response may regulate leukocyte localization to inflammatory sites. The primary structure of ICAM-1 has been found to be homologous to two cellular adhesion molecules, i.e., neural cell adhesion molecule (NCAM) and myelin-associated glycoprotein (MAG).
Several approaches to decreasing infectivity of viruses in general, and of rhinovirus in particular, have been pursued including: i) developing antibody to the cell surface receptor for use in blocking viral binding to the cell, ii) using interferon to promote an anti-viral state in host cells; iii) developing various agents to inhibit viral replication; iv) developing antibodies to viral capsid proteins/peptides; and v) blocking viral infection with isolated cell surface receptor protein that specifically blocks the viral binding domain of the cell surface receptor.
Using this last approach, Greve, et al., Cell, 56:879 (1989), supra, reported that purified tmICAM-1 could bind to rhinovirus HRV3 in vitro. Unpublished results with HRV2, HRV3, and HRV14 demonstrate a positive correlation between the ability to bind to rhinovirus and the ability to neutralize rhinovirus particularly if the binding studies are carried out under conditions where ICAM-1 is presented in a particular form and configuration as discussed further, infra. Results (unpublished) using HRV14 and HRV2 demonstrate a positive correlation between the receptor class of the virus and the ability to bind to tmICAM-1 in vitro. That is, ICAM-1, being the major receptor, can bind to HRV3, HRV14, and other "major" receptor serotypes and neutralize them, while it does not bind or neutralize HRV2, a "minor" receptor serotype. Further studies (unpublished), using purified tmICAM-1, demonstrate that it effectively inhibits rhinovirus infectivity in a plaque-reduction assay when the rhinovirus is pretreated with tmICAM-1 (50% reduction of titer at 10 nM receptor and one log reduction of titer at 100 nM receptor protein). These data were consistent with the affinity of rhinovirus for ICAM-1 of Hela cells, which had an apparent dissociation constant of 10 nM, and indicated a direct relationship between the ability of the receptor to bind to the virus and to neutralize the virus.
Because large-scale production of tmICAM-1 is not presently economically feasible, and because maintenance of tmICAM-1 in an active form requires the use of detergents, alternate means of producing a receptor protein for use as a rhinovirus inhibitor are desirable. Forms of the tmICAM-1 cDNA gene have been developed (as well as cell lines that produce the expression products; U.S. Ser. No. 07/390,662, now abandoned) that have been genetically altered to produce truncated ICAM-1 molecules. See FIG. 1. These truncated forms of ICAM-1 (tICAM(453) and tICAM(185)) lack the transmembrane region and are secreted into the cell culture medium. They bind to rhinovirus in the assay described in Greve, et al., Cell, 56:879 (1989), supra, although at substantially reduced levels relative to tmICAM-1. Thus, their effectiveness as inhibitors of rhinoviral infectivity appeared to be less than that of tmICAM-1. See generally co-pending applications U.S. Ser. No. 07/239,571, now abandoned; U.S. Ser. No. 07/262,428, now abandoned; U.S. Ser. No. 07/678,909, now abandoned; U.S. Ser. No. 07/631,313, now abandoned; U.S. Ser. No. 07/301,192, now U.S. Pat. No. 5,235,049; U.S. Ser. No. 07/449,356, now abandoned; U.S. Ser. No. 07/798,267, now abandoned; U.S. Ser. No. 07/556,238, now abandoned; U.S. Ser. No. 07/704,996, now abandoned; and U.S. Ser. No. 07/704,984, now abandoned.
U.S. Ser. No. 07/239,571, now abandoned, filed Sep. 1, 1988, and its CIP applications U.S. Ser. No. 07/262,428, now abandoned, U.S. Ser. No. 07/390,662 (abandoned in favor of continuation U.S. Ser. No. 07/678,909), U.S. Ser. No. 07/631,313, now abandoned, and U.S. Ser. No. 07/704,996, now abandoned, are directed to the use of transmembrane rhinovirus receptor as an inhibitor of rhinovirus infectivity using non-ionic detergent to maintain the transmembrane protein in solution, and directed to truncated intercellular adhesion molecules (tICAM) comprising one or more of the extracellular domains I, II, III, IV, and V of tmICAM, which truncated forms do not require the presence of non-ionic detergent for solubilization (see FIG. 1).
U.S. Ser. No. 07/130,378 filed Dec. 8, 1987 (abandoned in favor of continuation application U.S. Ser. No. 07/798,267), and CIP application U.S. Ser. No. 07/262,570 (now abandoned) are directed to transfected non-human mammalian cell lines which express the major rhinovirus receptor (HRR) and to the identification of HRR as intercellular adhesion molecule.
U.S. Ser. No. 07/301,192, now U.S. Pat. No. 5,235,049, filed Jan. 24, 1989, and its CIP application U.S. Ser. No. 07/449,356, now abandoned, are directed to a naturally-occurring soluble ICAM (sICAM) related to but distinct from tmICAM in that this sICAM lacks the amino acids spanning the transmembrane region and the cytoplasmic region; in addition this sICAM has a novel sequence of 11 amino acids at the C-terminus.
Subsequently, Marlin, S. D., et al., Nature, 344:70 (1990), reported the construction and purification of a truncated soluble form of the normally membrane-bound ICAM-1 molecule which they termed sICAM-1. It has both the transmembrane domain and the cytoplasmic domain of the protein deleted and differs from the wild-type amino acid sequence by a single conservative substitution at its carboxyl end. It is composed of residues 1-452 of ICAM-1 plus a novel phenylalanine residue at the C-terminus. These workers demonstrated that sICAM-1 was required at levels >50 .mu.g/ml to prevent the binding of HRV14 virus to cells. However, they also found that sICAM-1 at 1 .mu.g/ml (18 nM), when continually present in the culture medium, was able to inhibit by 50% the progression of an infection by HRV54. The inhibitory activity was correlated with the receptor class of the virus, in that Coxsackie A13 but not poliovirus or HRV2 was inhibited; infectivity data for HRV14 was not reported, however. Thus, they did not demonstrate a direct correlation between binding and inhibition of infectivity. Further, as discussed in greater detail, infra, attempts to reproduce the results obtained by Marlin, et al. have not been successful.
To date, no one has been able to demonstrate an agent that binds to and effectively reduces infectivity of human rhinovirus (by blocking viral infection with isolated cell surface receptor protein) as effectively as tmICAM-1; accordingly there continues to exist a need in the art for a form of ICAM-1 that can effectively bind to human rhinovirus and can effectively reduce HRV infectivity.
BRIEF SUMMARY OF THE INVENTION
Provided by the invention are multimeric configurations of transmembrane ICAM (tmICAM-1) and multimeric configurations of non-transmembrane ICAMs (tICAMs), having improved rhinovirus binding and inhibition activity.
As noted, supra, tmICAM-1 isolated from mammalian cells has the capacity to neutralize human rhinoviruses belonging to the major receptor group, but only if maintained in solution with detergent. Certain soluble fragments of ICAM-1 have been found to have a reduced capacity for binding virus and do not reduce infectivity as effectively as tmICAM-1. To date, no one has been able to ascertain the reason for this reduced capacity.
It has been proposed by others that the rhinovirus receptor exists on cells in a pentameric form [Tomassini, J., and Colonno, R., J. Virol., 58:290-295 (1986)]. However, quantitation (unpublished results of the co-inventors herein) of the rhinovirus and anti-ICAM-1 monoclonal antibody (Mab) binding to HeLa cells has revealed a maximum of 30,000 virions bound per cell (determined by the binding of [.sup.35 S]methionine-labeled HRV) and 50,000-60,000 ICAM-1 molecules per cell (determined by the binding of radio-labeled Mab to ICAM-1). These results prompted further studies to examine the possibility that rather than five, only between one and two ICAM-1 molecules on the surface of cells are bound per HRV particle bound to the cell.
Genetically engineered forms of truncated ICAM-1 that lack the C-terminal transmembrane domain are secreted into the culture medium of mammalian cells transfected with the recombinant gene. The purification of such secreted ICAM molecules from spent culture medium of cells stably transfected with the genes therefor is described herein. In a solution-HRV binding assay and in an HRV neutralization assay, it was found that the monomeric forms tend to have substantially reduced avidity for HRV relative to tmICAM-1. However, it has now been discovered that when such tICAMs are presented in multimeric form and then incubated with HRV, the virus-binding activity of the multimeric tICAMs becomes comparable to that of tmICAM-1. This binding of multimeric tICAMs to HRV has the same properties as the binding of HRV to ICAM-1 on HeLa cells: it is inhibited by anti-ICAM-1 Mabs, it is specific for rhinoviruses of the major receptor group, and has the same temperature dependence as the binding of rhinovirus to cells (i.e., binds well at 37.degree. C. and undetectably at 4.degree. C.). It is postulated that tmICAM exists in nature in a multimeric, possibly dimeric form, and that such constructs more closely resemble the native configuration, with its attendant high avidity for the human rhinovirus. Such dimerization may conveniently be achieved in vitro by, e.g., crosslinking two ICAM monomers by chemical means or by crosslinking with appropriate antibodies, or by binding monomers to appropriate inert substrates. Multimerization can also be achieved in vivo by modification of the gene sequence coding for the select ICAM to provide appropriate binding sites in the corresponding peptide sequence. For example, muteins can be engineered which contain appropriate cysteine residues to allow in vivo multimerization via interchain disulfide bonding. Alternatively, a DNA sequence coding for an ICAM may be fused with a DNA sequence coding for an appropriate immunoglobulin or fragment thereof, such that the fusion gene product possesses at least one site suitable for interchain bonding. The resulting fusion peptide monomer can then be expressed by the cell in multimeric form. Under certain circumstances, the benefits of multimerization may also be achieved by construction of ICAM muteins containing multiple rhinovirus binding sites.
Also provided by the invention are methods for enhancing binding of ICAM and functional derivatives thereof to a ligand, i.e., human rhinovirus, and "major" group receptor viruses, lymphocyte function-associated antigen-1 (LFA-1), Plasmodium falciparum (malaria) and the like, wherein the ICAM is presented in a multimeric configuration to the ligand to facilitate binding of the ICAM to the ligand.
The invention further comprises a method for inducing irreversible uncoating of human rhinovirus, said method comprising contacting said human rhinovirus with ICAM-1 or a fragment thereof.
This invention also provides a novel method of irreversibly inhibiting infectivity of a mammalian cell by a human rhinovirus, said method comprising contacting said human rhinovirus with ICAM-1 or a fragment thereof under conditions which allow the ICAM-1 or fragment thereof to bind to said rhinovirus; thereby stimulating irreversible uncoating of said rhinovirus.
Also provided by the invention are novel pharmaceutical compositions comprising a pharmaceutically acceptable solvent, diluent, adjuvant or carrier, and as the active ingredient, an effective amount of a polypeptide characterized by having human rhinovirus binding activity and reduction of virus infectivity. Dimeric configurations of ICAM and fragments thereof are presently preferred.
Other aspects and advantages of the present invention will be apparent upon consideration of the following detailed description thereof which includes numerous illustrative examples of the practice of the invention.

DESCRIPTION OF THE FIGURES
FIG. 1 is a schematic rendition of a) tmICAM-1, b) tICAM(453), c) tICAM(283), d) tICAM(185), and e) tICAM(88).
FIG. 2 is a schematic diagram of the constructs of Example 12: a) the heavy chain of human IgG; b) the fragment of the heavy chain used in making the immunoadhesin; c) the fragment of ICAM; d) the completed IgG/ICAM immunoadhesin.
FIG. 3 shows crosslinking of tICAM(453) into dimers by water-soluble carbodiimide/N-hydroxysuccinimide. tICAM(453) at the indicated concentrations was crosslinked with 100 mM EDC/5 mM NHS at pH 7.5 for 18 hr at 20 C. The samples were analyzed by SDS-PAGE followed by western blotting with anti-ICAM-1 antisera. a) Western blot of crosslinked ICAM(453) showing monomer and dimer species; b) dependence of crosslinking upon tICAM(453) concentration; c) the crosslinking of tICAM(453) is not inhibited by an excess of third-party proteins.
FIG. 4 is a schematic showing construction of tICAM(1-451)/LFA-3(210-237) chimera: a) tmICAM-1; b) tICAM(1-451); c) LFA-3; d) LFA-3(210-237); e) tICAM(1-451)/LFA-3(210-237) chimera; structure of tmICAM-1 shown for comparison.
FIG. 5 shows uncoating of HRV by tICAM(453) over 24 hours. a) shift from native 148S form to uncoated 42S form by tICAM(453); b) shift from native 148S to uncoated 42S form by tICAM(185); c)SDS-PAGE of [.sup.35 S]-methionine-labelled HRV-3 showing loss of VP4; d) dot-blot hybridization of RNA recovered from HRV3 species with an oligonucleotide probe for HRV. 50 ng of purified HRV3 RNA and RNA extracted from 8 ng of HRV3 species were applied to the blot.
FIG. 6 (SEQ ID NO:19) shows the predicted alignment of ICAM-1 amino acid sequence in domains IV and V onto the [SEQ ID NO:18] immunoglobulin fold motif. Arrows indicate beta strands, pointing from the N- to the C-terminus; italicized letters in bold indicate the beta strands, and numbered residues indicate cysteine residues with disulfide bonds indicated by lines. The dotted line divides the "B" and "F" faces of the domains. Residues indicated with an * are among those replaced with cysteine residues.

DETAILED DESCRIPTION OF THE INVENTION
As used herein, the following abbreviations and terms include, but are not necessarily limited to, the following definitions.
______________________________________Abbreviation Definition______________________________________ICAM Intercellular adhesion molecule may be used to denote both full length (trans- membrane) and truncated (non-trans- membrane) forms of the protein.ICAM-1 Intercellular adhesion molecule-1, also known as tmICAM-1 and HRR; denoting the full-length transmembrane proteintmICAM-1 Transmembrane intercellular adhesion molecule-1, also known as ICAM-1 and HRR; requires, e.g., detergent conditions to be solubilizedHRR Human rhinovirus receptor, also known as ICAM-1 and tmICAM-1sICAM-1 A naturally-occurring soluble truncated form of ICAM-1 having both the hydrophobic transmembrane domain and the carboxy-terminal cytoplasmic domain of ICAM-1 deleted; consists of amino acids 1-442 of ICAM-1 plus 11 novel amino acids; distinguishable from Staunton, et al. tICAM453 which consists of amino acids 1-453 with the terminal tyrosine replaced with phenylalanine.tICAMs Truncated intercellular adhesion molecules; soluble non-transmembrane ICAMs lacking the hydrophobic trans- membrane domain and the carboxyl- terminal cytoplasmic domain of ICAM-1.tICAM(1-453) Truncated form of ICAM comprising thetICAM-453 entire extracellular amino-terminaltICAM(453) domain of tmICAM (domains I-V, amino acid residues 1-453)tICAM(1-283) Truncated form of ICAM comprising domainstICAM-283 I, II, and III (amino acid residuestICAM(283) 1-283tICAM(1-185) Truncated form of ICAM comprising domainstICAM-185 I and II (amino acid residues 1-185)tICAM(185)tICAM(1-88) Truncated form of ICAM comprising domaintICAM-88 I (amino acid residues 1-88)tICAM(88)tICAM(89-185) Truncated form of ICAM comprising domain II (amino acid residues 89-185)tICAM(186-283) Truncated form of ICAM comprising domain III (amino acid residues 186-283)tICAM(284-385) Truncated form of ICAM comprising domain IV (amino acid residues 284-385)tICAM(386-453) Truncated form of ICAM comprising domain V (amino acid residues 386-453)tICAM(75-77) Truncated form of ICAM comprising amino acid residues 75-77)tICAM(70-72) Truncated form of ICAM comprising amino acid residues 70-72tICAM(64-66) Truncated form of ICAM comprising amino acid residues 64-66tICAM(40-43) Truncated form of ICAM comprising amino acid residues 40-43tICAM(36-38) Truncated form of ICAM comprising amino acid residues 36-38)tICAM(30-33) Truncated form of ICAM comprising amino acid residues 30-33)tICAM(26-29) Truncated form of ICAM comprising amino acid residues 26-29______________________________________
The foregoing terms defining specific fragments are intended to include functional derivatives and analogs thereof. Persons skilled in the art will understand that the given boundaries may vary by a few amino acid residues without affecting the function of the given fragment.
"Multimerization" and "multimeric" include, but are not limited to dimerization and dimeric, and include any multimeric configuration of the ICAM-1 molecule, or fragment thereof, that is effective in reducing viral binding and infectivity.
"Transmembrane" generally means forms of the ICAM-1 protein molecule which possess a hydrophobic membrane-spanning sequence and which are membrane-bound.
"Non-transmembrane" generally means soluble forms of the ICAM-1 protein including truncated forms of the protein that, rather than being membrane-bound, are secreted into the cell culture medium as soluble proteins, as well as transmembrane forms that have been solubilized from cell membranes by lysing cells in non-ionic detergent.
"Truncated" generally includes any protein form that is less than the full length transmembrane form of ICAM.
"Immunoadhesin" means a construct comprising all or a part of a protein or peptide fused to an immunoglobulin fragment, preferably a fragment comprising at least one constant region of an immunoglobulin heavy chain.
"Form" is generally used herein to distinguish among full length and partial length ICAM forms; whereas "configuration" is generally used to distinguish among monomeric, dimeric, and multimeric configurations of possible ICAM forms.
All forms and configurations of the ICAM-1 molecule, whether full length or a fragment thereof, including muteins and immunoadhesins, whether monomeric or multimeric, may be fully or partially glycosylated, or completely unglycosylated, as long as the molecule remains effective in reducing viral binding and infectivity.
"Ligand" is generally used herein to include anything capable of binding to at least one of any of the forms and configurations of ICAM and includes, but is not limited to, human rhinovirus, other viruses that bind to the "major" group human rhinovirus receptor, lymphocyte function-associated antigen-1, and Plasmodium falciparum (malaria).
"Human rhinovirus" generally includes all human serotypes of human rhinovirus as catalogued in Hamparian, V., et al., Virol., 159:191-192 (1987).
The sequence of amino acid residues in a peptide is designated in accordance with standard nomenclature such as that given in Lehninger's Biochemistry (Worth Publishers, New York, 1970).
Full-length ICAM-1, also known as human rhinovirus receptor (HRR), is termed transmembrane ICAM(tmICAM-1). Non-transmembrane ICAMs are also known as truncated ICAMS, i.e, ICAMs substantially without the carboxyl intracellular domain and without the hydrophobic membrane domain of tmICAM, which are soluble without the addition of detergent. tICAMs may conveniently comprise one or more domains selected substantially from domains I, II, III, IV, and V of the extracellular region of tmICAM. tICAMs may also comprise functional analogs of tmICAM or fragments thereof, and may also comprise one or more fragments of tmICAM spliced together, with or without intervening non-tmICAM linking sequences, and not necessarily in the same order found in native tmICAM. Presently preferred tICAMs include but are not limited to forms tICAM(453), tICAM(185), tICAM(88), tICAM(283), and tICAMs comprising one or more sequences selected from tICAM(89-185), tICAM(186-283), tICAM(284-385), tICAM(386-453), tICAM(75-77), tICAM(70-72), tICAM(64-66), tICAM(40-43), tICAM(36-38), tICAM(30-33), and tICAM(26-29). See U.S. Ser. No. 07/631,313, U.S. Ser. No. 07/678,909, and U.S. Ser. No. 07/704,996. Non-transmembrane forms of ICAM can include functional derivatives of ICAM, mutein forms of tICAM to facilitate coupling, and tICAM immunoadhesins. When the tICAMs are in a multimeric configuration, preferably as dimers, they display enhanced binding of human rhinovirus and are able to reduce viral infectivity.
Multimerization can be achieved by crosslinking a first ICAM to a second ICAM, using suitable crosslinking agents, e.g. heterobifunctional and homobifunctional cross-linking reagents such as bifunctional N-hydroxysuccinimide esters, imidoesters, or bis-maleimidohexanes.
The different forms of ICAM, transmembrane and non-transmembrane, can be multimerized by adsorption to a support. This support can be made of materials such as nitrocellulose, PVDF, DEAE, lipid polymers, as well as amino dextran, or a variety of inert polymers that can adsorb or can be coupled to ICAM, either with or without a spacer or linker.
Multimeric ICAM can also be multimerized by coupling the ICAM to a member, e.g., an antibody that does not interfere with HRV binding, or fragments thereof; or to a protein carrier. An example of an antibody includes anti-ICAM antibody CL 203 or a fragment thereof; suitable protein carriers include albumin and proteoglycans.
To facilitate coupling, the ICAM can be modified with at least one reactive amino acid residue such as lysine, cysteine, or other amino acid residue(s) to provide a site(s) to facilitate coupling. These types of modified ICAM are referred to as muteins. The nucleotide sequence for the ICAM of the method can be contained in a vector, such as a plasmid, and the vector can be introduced into a host cell, for example eukaryotic or prokaryotic cells. The preferred eukaryotic cell is a mammalian cell, e.g. Chinese hamster ovary cells or HEK293S cells; the preferred prokaryotic cell is E. coli. In addition, the ICAM can be modified at either terminus to comprise a lipid capable of promoting formation of oligomer micelles. The ICAM comprising the multimeric ICAM can be either fully glycosylated, partially glycosylated, or non- glycosylated.
A preferred manner of making multimeric forms of ICAM-1 is by engineering of cysteine residues into the tICAM sequence (tICAM(453) is particularly preferred) in a position at or close to the natural site of self-association on ICAM-1 monomers. Muteins with cysteine residues placed at appropriate positions form covalent bonds (disulfide bonds) that stabilize an interaction which is noncovalent in vivo. Such muteins are assembled intracellularly and are expressed as a disulfide-linked dimer; alternatively, monomeric muteins may be crosslinked in vitro by incubation at high protein concentration in mildly reducing conditions to encourage disulfide exchange, or by crosslinking with bifunctional chemical crosslinking reagents which react with free sulfhydryl groups. Another advantage of such proteins is that any novel amino acids engineered into ICAM-1 are hidden on the dimer interface and would be less likely to be immunogenic.
In another preferred embodiment, ICAM can also be multimerized by fusion with fragments of immunoglobulins to form ICAM immunoadhesins. For example, an ICAM or fragment thereof can be fused with a heavy or light chain immunoglobulin or fragment thereof, in particular with the constant region of the heavy chain of IgG, IgA, or IgM. Preferably, the constant region contains the hinge region and one or more of CH2 and CH3, but does not contain CH1. The variable region (Fab) of the immunoglobulin is thus replaced by the ICAM or fragment thereof. Such constructs are conveniently produced by construction and expression of a suitable fusion gene in a suitable expression system [see, e.g., Bebbington, C. R. and C. C. G. Hentschel, "The use of vectors based on gene amplification for the expression of cloned genes in mammalian cells," in DNA Cloning. Vol. III, D. Glover, ed.(1987)] and are secreted in a dimerized configuration.
Also provided by the invention are methods for enhancing binding of ICAM and functional derivatives thereof to a ligand, i.e., human rhinovirus, and "major" group receptor viruses, lymphocyte function-associated antigen-1 (LFA-1), Plasmodium falciparum (malaria) and the like, wherein the ICAM is presented in a multimeric configuration to the ligand to facilitate binding of the ICAM to the ligand.
The invention further comprises a method for inducing irreversible uncoating of human rhinovirus, said method comprising contacting said human rhinovirus with ICAM-1 or a fragment thereof, e.g. a tICAM as defined above.
This invention also provides a novel method of irreversibly inhibiting infectivity of a mammalian cell by a human rhinovirus, said method comprising contacting said human rhinovirus with ICAM-1 or a fragment thereof under conditions which allow the ICAM-1 or fragment thereof (e.g. a tICAM as defined above) to bind to said rhinovirus; thereby stimulating irreversible uncoating of said rhinovirus.
Also provided by the invention are novel pharmaceutical compositions comprising a pharmaceutically acceptable solvent, diluent, adjuvant or carrier, and as the active ingredient, an effective amount of a polypeptide characterized by having human rhinovirus binding activity and reduction of virus infectivity. Dimeric configurations of ICAM and fragments thereof are presently preferred.
The following examples illustrate practice of the invention.
Example 1 relates to growth, purification and assay of rhinoviruses;
Example 2 relates to production and isolation of monoclonal antibodies to ICAM-1;
Example 3 relates to construction of non-transmembrane truncated forms of ICAM cDNA from full length ICAM-1 cDNA;
Example 4 relates to transfection of mammalian-cells and expression of non-transmembrane truncated forms of ICAM cDNA;
Example 5 relates to isolation and purification of non-transmembrane truncated forms of ICAM-1;
Example 6 relates to radioactive labeling of tmICAM-1, tICAM(185), and tICAM(453) and demonstration of retained capacity for binding to monoclonal antibodies;
Example 7 relates to human rhinovirus binding assays of transmembrane and of non-transmembrane truncated forms of ICAM-1;
Example 8 relates to CL203 IgG antibody-mediated cross-linking of tICAM(453);
Example 9 relates to multimerization of trans-membrane and of non-transmembrane truncated forms of ICAM-1;
Example 10 relates to infectivity-neutralization assay of multimeric transmembrane and of multimeric non-transmembrane truncated forms of ICAM-1; and
Example 11 relates to use of multimeric forms of transmembrane and truncated forms of ICAM-1, as effective inhibitors of ICAM/LFA-1 interaction.
Example 12 relates to construction of tICAM(185)/IgG and tICAM(453)/IgG immunoadhesins.
Example 13 relates to rhinovirus binding and neutralization by a tICAM/IgG immunoadhesins.
Example 14 relates to in vitro dimerization of ICAM-1.
Example 15 relates to a tICAM(1-451)/LFA-3(210-237) chimera.
Example 16 relates to irreversible inactivation of HRV by ICAM.
Example 17 relates to cysteine muteins.
EXAMPLE 1
GROWTH, PURIFICATION AND ASSAY OF RHINOVIRUSES
Rhinoviruses were grown, purified, and assayed essentially as described in Abraham, G., et al., J. Virol., 51:340 (1984) and Greve, et al., Cell, 56:839 (1989). The serotypes chosen for these studies include HRV14, the standard in the field, and HRV3, which has an approximately 10-fold higher affinity for ICAM than does HRV14. HRV2, which binds to the "minor" receptor rather than the "major" receptor, was used as a negative control.
Rhinoviruses HRV2, HRV3, and HRV14 were obtained from the American Type Culture Collection, plaque purified, and isolated from lysates of infected HeLa-S3 cells. Purified rhinovirus was prepared by polyethylene glycol precipitation and sucrose gradient sedimentation. Viral purity was assessed by SDS-PAGE analysis of capsid proteins and by electron microscopy. Infectivity was quantitated by a limiting dilution infectivity assay scoring for cytopathic effect, essentially as described by Minor, P. D., Growth, assay and purification of picornaviruses, in Virology:A Practical Approach, B. W. J. Mahy, ed (Oxford:IRL Press), pp. 25-41.
EXAMPLE 2
PRODUCTION AND ISOLATION OF MONOCLONAL ANTIBODIES TO ICAM-1
BALB/cByJ female mice were immunized by intraperitoneal injection of 107 intact HeLa cells in 0.5 ml of phosphate-buffered saline (PBS) three times at 3-week intervals. Two weeks later the mice were bled and aliquots of serum were tested for protective effects against HRV14 infection of HeLa cells. Positive mice were boosted by a final injection of 10.sup.7 HeLa cells, and 3 days later spleen cells were fused to P3X63-Ag8.653 myeloma cells (Galfre, et al., Nature, 266:550-552 (1977)) to produce a total of approximately 700 hybridoma-containing wells. Each well was tested by incubating 3.times.10.sup.4 HeLa cells in 96-well plates with 100 .mu.l of supernatant for 1 hr at 37 C; the cells were then washed with PBS, and a sufficient amount of HRV14 was added to give complete cytopathic effect in 24-36 hr. Wells that were positive (protected from infection) were scored at 36 hr.
Cells were removed from wells which scored positive in the first screen and cloned by limiting dilution in 96-well microtiter plates. Supernatants from these wells were tested in the cell protection assay and positive wells were again identified. Further clonings were performed until all of the hybridoma containing wells were positive indicating a clonal population had been obtained. Four cloned cell lines, and their corresponding antibodies, were obtained and were designated c78.1A, c78.2A, c78.4A, c78.5A, c92.1A and c92.5A, respectively.
C92.1A was deposited on Nov. 19, 1987 with the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852 and was designated HB 9594.
EXAMPLE 3
CONSTRUCTION OF tICAM cDNAs FROM FULL LENGTH ICAM-1 cDNA
A. Preparation of ICAM-1 cDNA
Randomly-primed cDNA was synthesized from poly A+ RNA from HE1 cells using an Amersham(TM) cDNA synthesis kit under conditions recommended by the supplier. PCR amplification was performed using 100 ng of cDNA for 25 cycles using primers PCR 5.1: (ggaattcATGGCTCCCAGCAGCCCCCGGCCC) (SEQ ID NO:1) and PCR 3.1: (ggaattcTCAGGGAGGCGTGGCTTGTGTGTT) (SEQ ID NO:2). Amplification cycles consisted of 94 C 1 min, 55 C 2 min, and 72 C 4 min. The product of the PCR reaction was digested with EcoR1 and cloned with EcoR1 digested phage vector lambdaGT10 (Stratagene (TM)). Recombinant phage clones were screened by plaque hybridization using ICAM-1 specific oligonucleotides GAGGTGTTCTCAAACAGCTCCAGCCCTTGGGGCCGCAGGTCCAGTTC (SEQ ID NO:3) (ICAM1) and CGCTGGCAGGACAAAGGTCTGGAGCTGGTAGGGGGCCGAGGTGTTCT (SEQ ID NO:4) (ICAM3).
A positive clone designated lambdaHRR4 was selected and purified. The insert was removed by EcoR1 digestion and subcloned into the EcoR1 site of Bluescript KS+. This clone was designated pHRR2. The entire insert was sequenced and found to contain the entire ICAM-1 coding sequence beginning with the initiator ATG codon and ending with the TGA stop codon as specified by the PCR ICAM-1 sequence (Simmons, et al., Nature, 331:624 (1988); Staunton, et al., Cell, 52:925-933 (1988)[SEQ ID NO:20]) except for a single substitution of A-1462 for G. This same change was identified in several independent clones and thus represents a polymorphism of the ICAM-1 gene.
B. Construction of tICAM(453) and tICAM(185)
Modified forms of the ICAM-1 cDNA were created by PCR amplification reactions (Saiki, et al., Science, 230:1350-1354 (1985)) using the full length ICAM-1 cDNA clone pHRR-2 as template. The plasmid DNA was digested with EcoR1 to excise the ICAM-1 insert and treated with alkaline phosphatase to prevent re-circularization of the vector in subsequent ligation steps. Ten ng of template DNA was subjected to 10 cycles of PCR amplification using oligonucleotide primers PCR5.5 and PCR3.3 for tICAM-453 and PCR5.5 and 3.10 for tICAM-185 under the following conditions:
______________________________________Temperature (.degree. C.) Time (mins)______________________________________94 155 272 1.571 4 (final extension)______________________________________
PCR5.5 has the sequence: GGAATTCAAGCTTCTCAGCCTCGCTATGGCTCCCAGCAGCCCCCGGCCC (SEQ ID NO:5) which consists of EcoR1 and HindIII sites, 12bp ICAM-1 5' untranslated sequence, and the first 24 bp encoding the signal peptide.
PCR3.3 has the sequence: GGAATTCCTGCAGTCACTCATACCGGGGGGAGAGCACATT (SEQ ID NO:6) which consists of EcoR1 and Pst1 sites, a stop codon, and 24 bp complementary to the bases encoding the last 8 extracellular amino acids of ICAM-1 (residues 446-453).
PCR3.10 has the sequence: TTCTAGAGGATCCTCAAAAGGTCTGGAGCTGGTAGGGGG (SEQ ID NO:7) which consists of Xba1 and BamH1 sites, a stop codon, and 23 bp complementary to the bases encoding residues 178-185 of ICAM-1.
The PCR reaction products were digested with EcoR1 (tICAM(453)) or EcoR1 and BamH1 (tICAM(185)) and cloned into the polylinker site of Bluescript SK+(Stratagene). Clones containing the desired inserts were verified by restriction analysis and DNA sequencing. The inserts were excised from Bluescript by digestion with HindIII and XbaI and inserted into the expression vector CDM8 (Seed, Nature, 239:840 (1987) at the HindIII and XbaI sites. A clone containing the tICAM(453) insert designated pHRR-8.2 and a clone containing the tICAM(185) insert designated pHRR23-13 were selected and subjected to extensive sequence analysis. This verified the existence of the desired stop codons, and the integrity of the selected regions of ICAM-1 coding sequence.
These plasmids were transfected into COS cells using the DEAE-dextran techniques and the cells were cultured 72 hr. before assay. Surface expression was monitored by FACS using indirect immunofluorescence and a monoclonal antibody specific for ICAM-1. Transient expression in COS cells and immunoprecipitation of metabolically labelled ([.sup.35 S]cysteine) cell supernatants with c78.4A Mab (monoclonal antibody) demonstrated the production of soluble ICAM-1 fragments of 45 kd and 80 kd from pHRR23-13 and pHRR8.2, respectively. The preparation of stable Chinese hamster ovary cell transfectants is described below in Example 4.
C. Modified Non-glycosylated tICAM-1
A modified full length ICAM-1 was made by simultaneous mutagenesis of Asn at positions 103, 118, 156 and 175 each to Gln. This removes all four Asn-linked glycosylation sites from extracellular domain II of the ICAM-1 molecule. The resultant molecule, referred to as non-glycosylated transmembrane ICAM, was expressed on the surface of COS cells and was able to bind radio-labeled HRV3 at levels comparable to unmodified ICAM-1. This result demonstrated that glycosylation of domain II (the first 185 amino acids) is not required for virus binding to ICAM-1.
It is expected that non-transmembrane ICAM can be similarly modified to yield modified non-glycosylated non-transmembrane ICAM-1 molecules.
D. Construction of Genetically Engineered Forms of tICAM Containing Reactive Residues Suitable for Cross-Linking to Form Multimers.
A molecule consisting of the 453 amino acid extracellular domain of ICAM-1 with the addition of a novel lysine residue at the C-terminus was constructed by PCR modification of the pHRR-2 cDNA described in Example 3B. The primers used were PCR5.5 (Example 3B) and PCR 3.19 which has the sequence: TTCTAGAGGATCCTCACTTCTCATACCGGGGGGAGAGCACATT (SEQ ID NO:8) and consists of XbaI and BamHI sites, a stop codon, a Lys codon, and 24 bases complementary to the sequence encoding amino acid residues 446 to 453. Following cloning into the CDM8 vector, production of tICAM having a Lys at position 453 was confirmed by transient expression in COS cells. Stable CHO cell lines were generated by co-transfection with pSV2-DHFR as described in Example 4. The same strategy was used to add a Lys residue to the C-terminus of tICAM(185) using PCR5.5 and PCR3.20 which has the sequence: TTCTAGAGGATCCTCACTTAAAGGTCTGGAGCTGGTAGGGGGC (SEQ ID NO:9) and consists of XbaI and BamHI sites, a stop codon, a Lys codon, and 24 bases complementary to the sequence encoding residues 178 to 185. Transient COS cell expression confirmed the production of tICAM-185 and stable CHO cell lines were derived as described in Example 4.
Three modified forms of tICAM(452) that each contain an additional Cys residue were constructed by site-directed mutagenesis of the full-length ICAM-1 cDNA. In each construct a stop codon was introduced by changing the Glu residue at position 453 from GAG to TAG. The C-terminus is thus Tyr-452. Residues Asn-338, Thr-360, and Gln-387 were each separately mutated to Cys using a second site directed mutagenesis. The presence of the desired mutations were confirmed by DNA sequencing.
The residues selected for mutation to Cys were selected based on a computer generated plot of surface probability which predicts surface exposure of these regions. Also, Thr-360 is in close proximity to Asn-358 which is a site of potential Asn-linked glycosylation. Each of the three Cys mutants was expressed and secreted into the medium of transfected COS cells. Examination of the proteins under reducing and non-reducing conditions showed no indication of the presence of dimers. It is anticipated that cross-linking reagents reactive with sulfhydryl groups can be used to cross-link the Cys-modified tICAM forms to obtain multimeric forms.
EXAMPLE 4
TRANSFECTION OF CELLS AND EXPRESSION OF tICAM cDNA
A. Transfection of Eukaryotic Cells
Chinese hamster ovary (CHO) cells deficient in dihydrofolate reductase (DHFR) were obtained from Cutter Labs (Berkeley, Calif.). DHFR- cells cannot synthesize nucleosides and therefore require a nucleoside-supplemented medium. The cells were co-transfected with the plasmid pSV2-DHFR which contains the mouse dihydrofolate reductase (DHFR) gene under control of the SV40 promoter, and with tICAM(453), or tICAM(184) constructs in the CDM8 vector (Seed and Aruffo, PNAS, 84:3365-3369 (1987)).
Transfections were done using both electroporation and calcium phosphate methods. Bebbington, supra. Transfected DHFR-positive cells were selected by growth on nucleoside-free media, and pools of transfectants were cloned by limiting dilution.
Cell lines that secrete tICAM were identified by testing culture supernatants with a two-site radioimmune assay (RIA) for ICAM using Mabs c78.4A and c78.5A as follows. A monoclonal antibody against one epitope on ICAM (for example, Mab c78.4A) was adsorbed to plastic 96-well plates (Immunlon plates, Dynatech Inc.), excess binding sites on the plates were blocked with bovine serum albumin (BSA), and then culture supernatants were incubated with the plates. The plates were washed and incubated with [.sup.125 I]-Mab (directed against a second epitope on ICAM, e.g. c78.5A), and, after washing, the amount of bound [.sup.125 ]I-IgG determined. The concentration of tICAM was determined by comparing RIA data from unknowns against a standard curve of tmICAM at known concentrations. Positive clones were expanded and expression of tICAM forms was confirmed by immunoprecipitation of metabolically labeled cell supernatants with Mab c78.4A.
Cell lines CT0.2A (tICAM(453)) and CD12.1A (tICAM(185)) were selected for further study and were subjected to gene amplification in methotrexate containing media as described by Bebbington, et al., supra. A clone derived from CT0.2A resistant to 100 nM methotrexate and a CD12.1A clone resistant to 1 .mu.M methotrexate were used for purification of soluble truncated ICAM-1 proteins.
B. Transfection of Prokaryotic Cells
Because glycosylation of the viral binding domain of ICAM is not required to retain viral binding (as demonstrated in Example 3C), it is anticipated that prokaryotic cells, such as E. coli, can be successfully transfected to produce functional proteins.
EXAMPLE 5
ISOLATION AND PURIFICATION OF tICAM-1
Monoclonal antibody affinity chromatography with c78.4A-Sepharose(TM) has been previously described in co-pending U.S. Ser. No. 07/130,378 and Greve, et al., Cell, 56:839-847 (1989). tICAM secreted into serum-containing media required additional purification steps due to the high level of contaminating protein in the serum. Before elution from the Mab-affinity column, the column was washed with 1 M NaCl to remove loosely-bound proteins. For tICAM(453), the partially purified tICAM(453) eluted from the c78.4-Sepharose(TM) column was dialyzed into 10 mM Tris (pH 6.0), absorbed onto a mono-Q(TM) column (Pharmacia), and eluted with a 0-0.3 M NaCl gradient. tICAM184 was further purified by gel filtration on a Superose-12(TM) column.
It is also recognized that non-transmembrane truncated forms of ICAM-1 may be purified using standard ion exchange methodology without using monoclonal antibody affinity chromatography.
EXAMPLE 6
RADIOACTIVE LABELING OF tmICAM-1, tICAM(185), AND tICAM(453) AND DEMONSTRATION OF RETAINED CAPACITY FOR BINDING TO
MONOCLONAL ANTIBODIES
The epitopes reactive with monoclonal antibodies c78.4A and c78.5A are conformationally-dependent epitopes and thus can be used as analytical probes for confirming retention of the native ICAM structure. Known amounts of purified ICAM were incubated with c78.4A or c78.5A IgG-Sepharose(TM) and the fraction of the radioactivity bound determined. These experiments showed that the purified tmICAM-1, tICAM(185), and tICAM(453) completely retained the ability to bind to these monoclonal antibodies.
Transfectants were metabolically labeled with [.sup.35 S]cysteine, and cell lysates (for transmembrane ICAM) or culture supernatants (for truncated ICAM) were prepared and incubated with c78.4A IgG-Sepharose(TM) beads. The beads were washed and adsorbed proteins were eluted with sodium dodecyl sulfate (SDS) and analysed by SDS-PAGE; see Greve, et al., Cell, 56:839-847 (1989)). It was found that the isolated proteins were quantitatively bound to the c78.4A and c78.5A Mabs.
Accordingly, the tICAM(185) and tICAM(453) both have retained native ICAM structure.
EXAMPLE 7
HUMAN RHINOVIRUS BINDING ASSAYS OF tmICAM AND tICAMs
Described below are three binding assays used to assess binding activity of the various forms of ICAM.
A. Pelleting Assay
[.sup.35 S]cysteine-labeled tmICAM-1 or tICAM was mixed with HRV3 in 100 .mu.l of 10 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM MgCl.sub.2, 1 mM CaCl.sub.2, 0.1% Triton X-100. The mixture was incubated for 30 min. at 37 C, cooled on ice, layered on top of a cushion of 200 .mu.l of 10% glycerol, 0.2 M triethanolamine (pH 7.5), and centrifuged in a Beckman air-driven centrifuge at 134,000.times.g for 30 min. at 4 C. The top 275 .mu.l was removed, and the pellet was analyzed by SDS-PAGE and scintillation counting. Silver-staining of SDS gels of control experiments indicated that essentially all of the HRV3 is pelleted under these conditions and essentially all of the ICAM remains in the supernatant. The results are shown in Table 1.
TABLE 1______________________________________ICAM % ICAM Pelleted*______________________________________tmICAM-1 11.6%tICAM(453) 1.0%tICAM(185) 4.3%______________________________________ *average of 4 experiments; these numbers cannot be directly converted int relative affinities
These data show that both truncated forms of ICAM bind to rhinovirus, but at substantially reduced levels relative to tmICAM.
B. Solution Binding Assay
To obtain quantitative information on the relative affinity of tmICAM and tICAM fragments in solution, a solution competition assay was developed in which soluble tmICAM or soluble tICAM fragments were used to inhibit the binding of [.sup.35 S]HRV3 to previously immobilized ICAM-1; nonionic detergent (Triton X-100) was included in the buffers so that the different proteins could be compared under identical conditions. First, tmICAM-1 (isolated in the presence of 0.1% octylglucoside instead of Triton X-100) was diluted 10-fold into a Tris/NaCl buffer and allowed to adsorb to the walls of a microtiter plate (Immunlon-4, Dynatech) overnight. Nonspecific binding sites on the plate were then blocked with 10 mg/ml BSA and binding experiments performed in 0.1% Triton X-100/1 mg/ml BSA/10 mM Tris/200 mM NaCl. Approximately 20,000 cpm of [.sup.35 S]HRV3 were mixed with varying amounts of ICAM [tmICAM, tICAM(453) or tICAM(185)], incubated for 1 hour at 37 C, and then added to wells of the microtiter plates and incubated for 3 hr at 37 C. The plates were washed and the bound radioactivity determined.
As shown in Table 2, tmICAM-1 inhibits virus binding half-maximally at low concentrations (0.008 .mu.M) while tICAM(453) and tICAM(185) inhibit at much higher concentrations (2.8 .mu.M and 7.9 .mu.M, respectively; or 350 to almost 1000-fold higher than tmICAM.
TABLE 2______________________________________ICAM IC50*______________________________________tmICAM 8.0 .+-. 3.3 nM (N = 3)tICAM(453) 2.8 .+-. 0.6 .mu.M (N = 3)tICAM(185) 7.9 .+-. 2.8 .mu.M (N = 3)______________________________________ *IC50 is the concentration of soluble ICAM needed to inhibit HRV3 binding by 50%.
These data confirm and extend the earlier observations that tICAM(453) and tICAM(185) do bind to rhinovirus but with lower affinities than does tmICAM-1 and provide evidence that the virus binding site is encompassed within the two N-terminal domains (185 residues) of ICAM-1.
Subsequent experiments performed at 34 C (the temperature at which rhinovirus normally replicates) have yielded similar results.
C. Dot-Blot Assay
An alternative method of measuring binding activity was utilized in which tmICAM-1, tICAM(453), or tICAM(185) was adsorbed to nitrocellulose filters, the non-specific binding sites on the filters blocked with 10 mg/ml bovine serum albumin (BSA), and radioactive virus or [.sup.125 I]Mab to ICAM-1 incubated with the filter for 60 min at 37 C. The filters were washed with buffer and the filters exposed to X-ray film.
The amount of radioactivity bound to the filters was determined by densitometry of the autoradiograms, and the data is expressed as HRV3 binding (in arbitrary units) normalized to the amount of ICAM bound to the blot by a parallel determination of the amount of [.sup.125 I]Mab c78.4A or c78.5A bound to the ICAM (bound to the blot). The results are shown in Table 3.
TABLE 3______________________________________Binding of [.sup.35 S]HRV3 to Immobilized ICAM*ICAM tICAM(453) ratio ICAM/tICAM453______________________________________1.2 .+-. 1.1 0.52 .+-. 0.45 2.3______________________________________ *Average of 5 experiments. Data is expressed in arbitrary densitometric units of [.sup.35 S]HRV3 binding/[.sup.125 ]IantiICAM Mab binding.
Additional studies with tICAM 185 have been performed. Binding experiments have demonstrated equivocal results. It is anticipated that steric hindrance may play a role. The size of the virus is approximately 30 nanometers. The length of tICAM(185) is less than 10 nanometers. The use of a spacer or linker would provide better accessibility for binding.
The results from this experiment indicate that under these assay conditions tICAM(453) is capable of binding rhinovirus at levels comparable to those of tmICAM-1 when the amount of virus bound was normalized to the amount of [.sup.125 I]MAb bound. Further, these results indicate that the tICAM forms are capable of binding to rhinovirus, but that the binding avidity is dependent upon the configuration of the tICAM. tmICAM-1 is believed to be a small multimer (probably a dimer) and presentation of tICAM in a multimeric form mimics this multimeric configuration.
Evidence supporting this hypothesis comes from quantitative binding studies (unpublished), in which the ratio of the maximum number of rhinovirus particles and the maximum number of antibody molecules that can be bound to cells is approximately 1.5, as discussed supra. This is in contrast to the earlier work of Tomassini, J., et al., J. Virol., 58:290 (1986), which suggested a complex of five molecules needed for binding. Their conclusion was based on an erroneous interpretation of gel filtration data that failed to take into account bound detergent molecules.
EXAMPLE 8
CL203 IgG ANTIBODY-MEDIATED CROSS-LINKING OF tICAM(453)
To provide additional evidence that the higher relative binding activity of tmICAM-1 is due to a multimeric form of the protein, the tICAM(453) protein was pre-incubated with CL203, a monoclonal antibody to ICAM-1 that does not inhibit virus binding to ICAM-1 and binds to a site C-terminal to residue 184 (Staunton, et al., Cell, 56:849 (1989) and Cell, 61:243 (1990)). Thus, the antibody can effectively "cross-link" two molecules of tICAM(453), to create "dimers" of tICAM(453), yet without blocking the virus-binding site on each of the two molecules of tICAM(453). When a mixture of CL203 IgG and tICAM(453) at a 4:1 weight ratio was tested in the competition assay, it was found that the antibody cross-linked tICAM(453) inhibited HRV3 binding at a concentration 7.4-fold lower than tICAM(453) alone consistent with the idea that tmICAM-1 binds with higher affinity to rhinovirus because it is a dimer or a small multimer.
To create alternative multimeric forms of tICAM, several further modified truncated forms of ICAM were constructed as described, supra, in Example 3.
These forms can then be multimerized as described in Example 9, below.
EXAMPLE 9
MULTIMERIZATION OF tmICAM AND tICAMs
There are several ways that tICAM can be converted to a multimeric form having enhanced viral binding and neutralization activity over the monomeric form. For example, a first tICAM can be coupled to a second tICAM(which may be the same or different), or to an inert polymer, such as amino-dextran (MW 40,000), using homobifunctional (such as N-hydroxysuccinimide (NHS) esters) or heterobifunctional (such as those containing NHS-ester and photoactivatable or sulfhydryl-reactive groups) cross-linking reagents utilizing the amino group on the amino-dextran and an amino or other group on the tICAM. A number of examples of appropriate cross-linking reagents can be found in the Pierce Chemical Company catalog (Rockford, Ill.). Similarly, the tICAMs can also be bound to other suitable inert polymers, such as nitrocellulose, PVDF, DEAE, lipid polymer, and other inert polymers that can adsorb or be coupled to tICAM with or without a spacer or linker.
As tICAM is poorly reactive with NHS-ester-based compounds, a tICAM with a genetically-engineered C-terminal lysine residue (see Example 3) would have improved coupling efficiency to supports with homobifunctional reagents whereas genetically-engineered C-terminal cysteine residues would facilitate coupling by heterobifunctional reagents, such as sulfo-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS).
ICAMs can also be multimerized by coupling with an antibody (e.g. CL203) or fragment thereof, or with a suitable protein carrier, e.g. albumin or proteoglycan.
ICAMs may also be multimerized by fusion with fragments of immunoglobulins to form ICAM immunoadhesins.
Alternatively, soluble tICAM multimers can be created by genetically engineering reactive residues into tICAM. For example, free cysteine residues can be created in relatively hydrophilic sequences in the C-terminal region of tICAM (which would have a greater tendency to be solvent-exposed). This will allow the creation of dimers in situ; alternatively, monomers can be purified and dimers created in vitro by disulfide bonding, either directly or via suitable linkers.
Another approach requires the placement of lysine residues at similar positions and cross-linking purified protein in vitro with homobifunctional NHS-esters. Examples of such lysine residues are residues 338, 360, 387.
Crosslinking cysteine residues to each other can be accomplished by reaction of tICAM with free cysteine groups with bis-maleimidohexane (Pierce Chemical Co.) or other bis-maleimido-analogs. Cross-linking free cysteine residues on tICAM to amino groups on carrier molecules can be accomplished by reaction with m-maleimidobenzoyl-N-hydroxy-succinimide ester.
Crosslinking amino groups on tICAM molecules can be accomplished with homobifunctional N-hydroxysuccinimide esters (for examples, see Pierce Chemical Co. catalog). Alternatively, the carbohydrate groups on tICAM can be oxidized to aldehydes and coupled to hydrazine-activated amino groups on a carrier molecule.
EXAMPLE 10
INFECTIVITY-NEUTRALIZATION ASSAY OF tmICAM AND tICAMs
Three different assays for virus infectivity have been used. These different assays take into account the differences in transmembrane ICAM and non-transmembrane solubilities.
A. Plaque-Reduction Assay in the Presence of Detergent
The results of this assay indicate the highest dilution of virus that will still be effective in killing cells. Virus is pre-incubated with transmembrane ICAM protein in the presence of 0.1% Triton X100, serially diluted into culture medium, incubated for 30 min with HeLa cells at 10.sup.6 cells/ml, diluted 10-fold, and plated out into multiple wells of a 96-well microtiter plate having varying dilutions of virus.
0.1% Triton X100 was used as positive control. After 5 days, the wells are scored as either being infected or not by the presence of cytopathic effect (CPE) and the titer expressed as plaque-forming units/ml (PFU/ml) of the original virus. This assay was described in U.S. Ser. No. 07/239,571 and was used to demonstrate the antiviral activity of tmICAM-1 (which required the presence of detergent to remain in solution). The concentration of ICAM protein used is the initial concentration in the pre-incubation mixture; however, the ICAM protein is not present continually during the infection in that the protein is serially diluted. While the presence of detergent is required to solubilize the tmICAM, detergent kills the cells; thus, the need for the serial dilutions of the tmICAM-1/detergent to permit infection of cells.
B. Plaque-Reduction Assay in the Absence of Detergent
In this plaque-reduction assay, a more traditional assay, HeLa cells are infected with serial dilution of rhinovirus as above, but detergent is not present; thus, this assay cannot be used to assay tmICAM. In this assay the tICAM is present continually in the culture medium at the indicated concentration. tmICAM-1 (which requires the presence of detergent) cannot be assayed in this system because the addition of the required detergent would kill the HeLa cells.
C. Plaque-Reduction Assay in Continual Presence of Virus and ICAM
This assay is similar to that utilized by Marlin, et al. (Nature 1990) in which a culture of HeLa cells is infected with 100 PFU of virus in the presence or absence of ICAM protein and cultured approximately 4 days until cytopathic effect (CPE) is apparent. The cultures are then scored for CPE visually. The assay conditions were the same as Marlin, supra. Scoring was done visually rather than by a staining procedure using crystal violet.
In this assay, there is no detergent present, the ICAM is present continually, and this assay measures a reduction in virus replication/propagation at an arbitrary point in time.
The data from these three different assays for virus infectivity is summarized in Table 4.
TABLE 4______________________________________ IC50% (.mu.M)*ICAM Assay: A B C______________________________________tmICAM-1 0.03 NDtICAM(453) >20 0.2 0.2tICAM(185) >20 8 ND______________________________________ *IC50% is defined as the concentration of ICAM protein needed to inhibit HRV3 infectivity by 50%.
These data indicate that tmICAM-1 is significantly more active in reducing viral infectivity than the truncated ICAM proteins, even when compared in different assay systems. The differences in neutralization activity of tICAM(453) in assay (A) and assay (B) indicate that the neutralization mediated by tICAM(453) requires the continual presence of tICAM(453) in the culture medium and is reversible. That the neutralization is reversible is indicated by the lack of significant neutralization observed in assay (A). In contrast, the neutralization activity of tmICAM-1 is >667-fold higher than tICAM(453) and than tICAM(185) in assay (A) and could be even greater in assay (B) if it were possible to have the tmICAM-1 present continually in the culture medium in the absence of detergent. The conditions in assays B-D more closely reflect the in vivo situation in which soluble ICAM could be used as an antiviral agent.
To compare these results with those of Marlin et al., an attempt was made to reproduce their assay conditions. As shown in Table 4, there is a good correlation between the results in assay (B) and assay (C), although the IC50% for tICAM(453) is 10-fold greater than that seen by Marlin et al. To determine if this is due to a difference in the serotype of rhinovirus used, the assay was repeated with HRV14 and HRV54 (the serotype used by Marlin, et al.). The IC50% for both of these serotypes was 0.2 .mu.M tICAM(453), indicating that there is no difference in serotype sensitivity between HRV14, HRV54, and HRV3.
To attempt to resolve this discrepancy, the same buffers that Marlin, et al. used were used to see if they affected the infectivity of rhinovirus in assay (C). Marlin, et al. prepared their sICAM-1 protein in a buffer containing 50 mM triethanolamine (TEA)/20 mM Tris. When this buffer alone was added to control infections (1/10th volume, final concentration 5 mM TEA/2 mM Tris) of HRV3 and HRV14, virtually complete inhibition of CPE was observed. Thus, it is possible that there could be buffer effects on virus replication unrelated to the presence of any form of ICAM.
However, subsequent assays using a broad panel of HRV serotypes indicates that the IC50% for HRV54 may in fact be significantly lower than for other HRV serotypes, e.g. HRV3.
EXAMPLE 11
USE OF MULTIMERIC FORMS OF tmICAM AND tICAMs AS EFFECTIVE INHIBITORS OF ICAM/LFA-1 INTERACTION
The normal function of ICAM-1 is to serve as a ligand of the leukocyte integrin LFA-1; interaction between these two molecules leads to adhesion between leukocytes and a variety of other cells. The ability of tICAMs to inhibit adhesion between ICAM-1 and LFA-1 on cells was examined as follows. ICAM-1 was adsorbed to microtiter plates as described in Example 7C. JY cells, which express LFA-1, adhere to ICAM-expressing cells or to ICAM-1-coated culture dishes (Staunton, et al., JCB). JY cells (10.sup.7 cell/ml in 10 mM HEPES pH 7.5/150 mM NaCl/1 mM CaCl.sub.2 /1 mM MgCl.sub.2 containing 1 mg/ml BSA) labeled with 10 .mu.Ci/ml [.sup.36 S]-cysteine for 18 hours) were pre-incubated in the presence or absence of tICAM(453) or tICAM(185) for 30 min at 37 C, and then added to the ICAM-1-coated plates and incubated for 60 min at 37 C. The microtiter plates were then washed three times with media, and the number of cells bound to the plates were quantified by scintillation counting.
As shown in Table 5, tICAM(185) and tICAM(453) both inhibited JY cell binding at identical concentrations of between 5 and 20 .mu.M.
TABLE 5______________________________________% JY Cell Binding.mu.M ICAM-1 tICAM(453) tICAM(185)______________________________________20 1006 52 47 500.6 83 720.02 86 800.006 89 97______________________________________ *Binding to ICAM1-coated microtiter plates; 10 .mu.g/ml antiLFA-1 or antiICAM-1 MAb inhibited binding to <1%.
EXAMPLE 12
CONSTRUCTION OF tICAM/IgG IMMUNOADHESINS
A soluble derivative of ICAM-1 was constructed by a cDNA fusion which linked the first two domains of ICAM-1 (residues 1-185) to a segment of human immunoglobulin heavy chain cDNA. This approach has been described previously for the CD4 molecule [Zettlmeissl, G., J-P Gregersen, J. M. Duport, S. Mehdi, G. Reiner, and B. Seed, "Expression and Characterization of Human CD4: Immunoglobulin Fusion Proteins", DNA and Cell Biology (1990) 9(5):347-353; Capon, D. J., S. M. Chamow, J. Mordenti, S. A. Marsters, T. Gregory, H. Mitsuya, R. A. Bryn, C. Lucas, F. M. Wurm, J. E. Groopman, S. Broder, and D. H. Smith, "Designing CD4 immunoadhesins for AIDS therapy", Nature (1989) 337:525-531; Traunecker, A. J. Schneider, H. Kiefer and K. Karjalainen, "Highly efficient neutralization of HIV with recombinant CD4-immunoglobulin molecules", Nature (1989) 339:68-70] and resulted in the expression of disulfide-linked dimers.
The cDNA fusion was accomplished by a two-stage polymerase chain reaction (PCR) strategy. [ee, e.g., Horton, R. M., Z. Cai, S. N. Ho, and L. R. Pease, "Gene Splicing by Overlap Extension: Tailor-Made Genes Using the Polymerase Chain Reaction", BioTechniques (1990) 8(5):528-535]. The first step involved the separate amplification of a fragment coding for residues 1-185 of ICAM-1 and an IgG heavy chain fragment beginning at residue 216 in the hinge region and ending at the C-terminus of the molecule (see FIG. 2). The PCR primer used at the 3' end of the ICAM-1 fragment contained an additional 24 bases complementary to the first 24 bases of the IgG fragment: CGG TGG GCA TGT GTG AGT TTT GTC AAA GGT CTG GAG CTG GTA GGG GGC (SEQ ID NO:10). The 5' ICAM-1 primer (5' noncoding and signal sequence) had the sequence:
HindIIIGGA ATT CAA GCT TCT CAG CCT CGC TAT GGC TCC CAG CAG CCC CCG (SEQ ID NO: 5)GCC C
The 5' IgG primer had the following sequence: GAC AAA ACT CAC ACA TGC CCA CGG (SEQ ID NO:11); the 3' primer from the end of the IgG coding sequence was:
XbaIG GGA TTC TCT AGA TCA TTT ACC CGG AGA CAG GGA GAG GCT (SEQ ID NO: 12)
Amplifications were performed using 10 ng of cloned ICAM-1 or IgG1 heavy chain cDNA for 10 cycles with 1 min at 94 C, 2 min at 55 C and 1.5 min extensions at 72 C. The resulting amplified fragments were mixed in approximately equimolar amounts and used as template for the second step PCR reaction. This reaction used the 5' ICAM primer and the 3' IgG primer above. Amplification for 25 cycles under the same conditions as in the first step produced a predominant band of approximately 1200 bp consistent with the desired product (see FIG. 2). The fragment was digested with HindIII and XbaI (restriction sites incorporated into the 5' and 3' primers respectively), purified and ligated into HindIII/XbaI-cleaved CDM8 vector.
Clones containing the desired insert were identified by restriction analysis and two clones designated pHRR72 and pHRR73 were selected for sequence analysis. Sequencing of the junction region between ICAM-1 and the IgG hinge confirmed that both clones had the correct structure. The plasmids were transfected into COS cells which were labelled with [.sup.35 S]cysteine overnight at 48 hours post-transfection as in Example 6. The supernatants were immunoprecipitated with anti-ICAM-1 monoclonal antibody c78.4A and analyzed by SDS gel electrophoresis as in Example 6. Under reducing conditions a band with an apparent molecular weight of 68 kD was specifically immunoprecipitated, corresponding to the ICAM-1/IgG fusion. Expression of clone pHRR72 was consistently higher than pHRR73 so this clone was selected for further study.
COS cells were transfected with pHRR72 according to the method of Example 3 and at 48 hours after transfection the media was replaced with serum-free media containing [.sup.35 S]cysteine and the cells were labelled overnight as above. The supernatants were incubated with protein A-Sepharose beads, and bound protein was eluted with 0.1 M acetic acid, neutralized and analyzed by gel electrophoresis under reducing and non-reducing conditions. A control was performed in which plasmids expressing heavy and light chains of a functional antibody were co-transfected. This experiment showed that the protein produced by pHRR72 is capable of binding protein A, showing that the pHRR72 protein contains the IgG constant region, and that the 68 kD band seen under reducing conditions shifts to a high molecular weight dimeric form under non-reducing conditions. Thus since only dimeric IgG binds protein A, and since the mobility under non-reducing conditions is at least twice that of the monomer, we conclude that the tICAM(185)/IgG immunoadhesin is a dimer. Correct folding of the ICAM-1 region is indicated by the specific immunoprecipitation with c78.4A as in Example 6, and by the quantitative detection of the fusion protein using two ICAM-1-specific antibodies in a radioimmune assay (RIA) as in Example 4.
pHRR72 was co-transfected with pSV2-DHFR into CHO cells by the calcium phosphate method of Example 4 and DHFR+cells were selected in nucleoside-free medium. Individual colonies were picked, expanded and tested by RIA for expression. The three highest-expressing colonies were selected for further study and were recloned by limiting dilution. Analysis of labelled cell supernatants by protein A binding and gel electrophoresis confirmed the expression of tICAM(185)/IgG dimers.
In a similar manner, domains I-V of ICAM-1 (residues 1-453) were linked to a segment of human immunoglobulin heavy chain cDNA. A fragment coding for residues 1-453 of ICAM-1 and a fragment coding for IgG heavy chain beginning at residue 216 in the hinge region and ending at the C-terminus of the molecule were each separately amplified. The PCR primer used at the 3' end of the ICAM-1 fragment contained an additional 24 bases complementary to the first 24 bases of the IgG fragment: CGG TGG GCA TGT GTG AGT TTT GTC CTC ATA CCG GGG GGA GAG CAC ATT (SEQ ID NO:13). The 5' ICAM-1 primer, 5' IgG primer, and 3' primer from the end of the IgG coding sequence were the same as for the tICAM(185)IgG fusion above. After PCR amplification, a band of approximately 2000 bp consistent with a tICAM(453)/IgG fusion was produced.
Clones containing the desired insert were identified by restriction analysis and the clone designated pHRR 95-9 was selected for sequence analysis. The cDNA sequence is as follows:
CAGACATCTG TGTCCCCCTC AAAAGTCATC CTGCCCCGGG GAGGCTCCGT (SEQ ID NO: 14)51 GCTGGTGACA TGCAGCACCT CCTGTGACCA GCCCAAGTTG TTGGGCATAG101 AGACCCCGTT GCCTAAAAAG GAGTTGCTCC TGCCTGGGAA CAACCGGAAG151 GTGTATGAAC TGAGCAATGT GCAAGAAGAT AGCCAACCAA TGTGCTATTC201 AAACTGCCCT GATGGGCAGT CAACAGCTAA AACCTTCCTC ACCGTGTACT251 GGACTCCAGA ACGGGTGGAA CTGGCACCCC TCCCCTCTTG GCAGCCAGTG301 GGCAAGAACC TTACCCTACG CTGCCAGGTG GAGGGTGGGG CACCCCGGGC351 CAACCTCACC GTGGTGCTGC TCCGTGGGGA GAAGGAGCTG AAACGGGAGC401 CAGCTGTGGG GGAGCCCGCT GAGGTCACGA CCACGGTGCT GGTGAGGAGA451 GATCACCATG GAGCCAATTT CTCGTGCCGC ACTGAACTGG ACCTGCGGCC501 CCAAGGGCTG GAGCTGTTTG AGAACACCTC GGCCCCCTAC CAGCTCCAGA551 CCTTTGTCCT GCCAGCGACT CCCCCACAAC TTGTCAGCCC CCGGGTCCTA601 GAGGTGGACA CGCAGGGGAC CGTGGTCTGT TCCCTGGACG GGCTGTTCCC651 AGTCTCGGAG GCCCAGGTCC ACCTGGCACT GGGGGACCAG AGGTTGAACC701 CCACAGTCAC CTATGGCAAC GACTCCTTCT CGGCCAAGGC CTCAGTCAGT751 GTGACCGCAG AGGACGAGGG CACCCAGCGG CTGACGTGTG CAGTAATACT801 GGGGAACCAG AGCCAGGAGA CACTGCAGAC AGTGACCATC TACAGCTTTC851 CGGCGCCCAA CGTGATTCTG ACGAAGCCAG AGGTCTCAGA AGGGACCGAG901 GTGACAGTGA AGTGTGAGGC CCACCCTAGA GCCAAGGTGA CGCTGAATGG951 GGTTCCAGCC CAGCCACTGG GCCCGAGGGC CCAGCTCCTG CTGAAGGCCA1001 CCCCAGAGGA CAACGGGCGC AGCTTCTCCT GCTCTGCAAC CCTGGAGGTG1051 GCCGGCCAGC TTATACACAA GAACCAGACC CGGGAGCTTC GTGTCCTGTA1101 TGGCCCCCGA CTGGACGAGA GGGATTGTCC GGGAAACTGG ACGTGGCCAG1151 AAAATTCCCA GCAGACTCCA ATGTGCCAGG CTTGGGGGAA CCCATTGCCC1201 GAGCTCAAGT GTCTAAAGGA TGGCACTTTC CCACTGCCCA TCGGGGAATC1251 AGTGACTGTC ACTCGAGATC TTGAGGGCAC CTACCTCTGT CGGGCCAGGA1301 GCACTCAAGG GGAGGTCACC CGCAAGGTGA CCGTGAATGT GCTCTCCCCC1351 CGGTATGAG g acaaaactca cacatgccca ccgtgcccag cacctgaact1401 cctgggggga ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc1451 tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc1501 cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt1551 gcataatgcc aagacaaagc cgcgggagga gcagtacaac agcacgtacc1601 gggtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag1651 gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa1701 aaccatctcc aaagccaaag ggcagccccg agaaccacag gtgtacaccc1751 tgcccccatc ccgggatgag ctgaccaaga accaggtcag cctgacctgc1801 ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa1851 tgggcagccg gagaacaact acaagaccac gcctcccgtg ctggactccg1901 'acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg1951 cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa2001 ccactacacg cagaagagcc tctccctgtc tccgggtaaa tga (wherein UPPERCASE indicates nucleotides coding for amino acid residues 1-453 of ICAM1, and lowercase indicates nucleotides coding from amino aci residues 216-442 of human heavy chain IgGl)
The corresponding amino acid sequence of the mature fusion polypeptide is as follows:
1 QTSVSPSKVI LPRGGSVLVT CSTSCDQPKL LGIETPLPKK ELLLPGNNRK (SEQ ID NO: 15)51 VYELSNVQED SQPMCYSNCP DGQSTAKTFL TVYWTPERVE LAPLPSWQPV101 GKNLTLRCQV EGGAPRANLT VVLLRGEKEL KREPAVGEPA EVTTTVLVRR151 DHHGANFSCR TELDLRPQGL ELFENTSAPY QLQTFVLPAT PPQLVSPRVL201 EVDTQGTVVC SLDGLFPVSE AQVHLALGDQ RLNPTVTYGN DSFSAKASVS251 VTAEDEGTQR LTCAVILGNQ SQETLQTVTI YSFPAPNVLL TKPEVSEGTE301 VTVKCEAHPR AKVTLNGVPA QPLGPRAQLL LKATPEDNGR SFSCSATLEV351 AGQLIHKNQT RELRVLYGPR LDERDCPGNW TWPENSQQTP MCQAWGNPLP401 ELKCLKDGTF PLPIGESVTV TRDLEGTYLC RARSTQGEVT RKVTVNVLSP451 RYEDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS501 HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK551 EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC601 LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW651 QQGNVFSCSV MHEALHNHYT QKSLSLSPGK *
The plasmids were transfected into COS cells which were labelled with [.sup.35 S]cysteine overnight at 48 hours post-transfection as in Example 6. The fusion polypeptide is expressed as a soluble secreted disulfide-linked dimer which binds protein A. The supernatants were immunoprecipitated with anti-ICAM-1 monoclonal antibody c78.4A and analyzed by SDS gel electrophoresis as in Example 6. Under reducing conditions a band with an apparent molecular weight of 100 kD was specifically immunoprecipitated, corresponding to the ICAM-1/IgG fusion, while under non-reducing conditions it migrates as a 200 kD dimer.
EXAMPLE 13
RHINOVIRUS BINDING AND NEUTRALIZATION BY tICAM/IgG
IMMUNOADHESINS
The tICAM(185)/IgG immunoadhesin of Example 12 consists of residues 1-185 of ICAM-1 fused to residue 216 in the hinge region of an IgGl heavy chain. The molecule is a disulfide-linked dimer containing two rhinovirus binding sites. A CHO cell line CHO72.2 secreting the immunoadhesin was grown overnight in serum-free media containing [.sup.35 S]cysteine and the fusion protein was purified on protein A beads. The labelled protein was tested for rhinovirus binding in the pelleting assay as described in Example 7(A). The samples consisted of tICAM(185)/IgG (no virus), tICAM(185)/IgG+HRV3, tICAM(185)/IgG+HRV3+c78.4A, and tICAM(185)/IgG+HRV3+irrelevant antibody. Pelleting of labelled protein indicative of virus binding was seen with virus and virus+irrelevant antibody by analysis on SDS gels. No pelleting was seen in the absence of virus and significantly reduced pelleting was seen in the sample containing c78.4A. This result indicates that the tICAM(185)/IgG binds rhinovirus with a significantly higher affinity than the soluble monomers tICAM(185) and tICAM(453), which do not show levels of binding readily detectable under these conditions. See Example 7(A). While approximately 10% of tmICAM-1 pellets under these conditions, only 1% of tICAM(453) pellets, presumably because tmICAM-1 is in a dimeric state. The result with tICAM(185)/IgG is similar to that seen in this assay with tmICAM-1, suggesting that the two forms of ICAM may have similar affinities for the virus, and providing further evidence that tmICAM-1 is a dimer.
Cell supernatant from CHO72.2 cells containing unpurified tICAM(185)/IgG was tested for rhinovirus neutralization in a virus infectivity assay according to the method of Example 10(B). Serial dilutions of HRV3 were made in media containing 50% IgG supernatant or control supernatant from untransfected CHO cells. The virus dilutions were mixed with HeLa cells and plated in wells of a 96-well microtiter plate (10 wells per dilution). Virus titers were determined by scoring the number of infected wells at each dilution after 6 days. In addition a quantitative assessment of cytopathic effect at high virus input was made 2 days after infection. In experiment A the concentration of tICAM(185)/IgG estimated by RIA was 150 ng/ml and in experiment (B) the concentration was 325 ng/ml.
TABLE 6______________________________________ Experiment A Experiment B______________________________________HRV3 1 .times. 10.sup.7 PFU/ml 4 .times. 10.sup.6 PFU/mlHRV3 + 6 .times. 10.sup.5 PFU/ml 5 .times. 10.sup.5 PFU/mltICAM(185)/IgG______________________________________
Both experiments resulted in a ten-fold reduction in viral titer at a concentration of approximately 1 nM in experiment A and 2 nM in experiment B. For comparison, monomeric tICAM(453) in the same assay results in a 50% reduction in titer at 0.38 .mu.M or 30 .mu.g/ml. Thus the increase in activity resulting from dimerization of the rhinovirus binding site is at least 200-fold and probably greater.
Cell supernatant from CHO72.2 at a concentration of 650 ng/ml (4 nM) was also tested in a competitive binding assay measuring the binding of [.sup.35 S]HRV3 to ICAM-1-coated plastic microtiter wells. Specific binding is determined by comparing counts bound with or without pre-incubation of the ICAM-1 in the well with Mab c78.4A.
TABLE 7______________________________________ cpm bound* % binding______________________________________HRV3 4945 +/- 58 100HRV3 + CHO supernatant 5358 +/- 51 108HRV3 + CHO72.2 supernatant 3187 +/- 206 64______________________________________ *Mean values determined from triplicate wells. Standard errors were less than 10% of the mean.
The level of binding in the presence of tICAM(185)/IgG was 65% of the normal control binding and 54% of control binding in the presence of CHO cell supernatant, indicating close to a 50% inhibition of binding. For comparison, soluble monomeric tICAM(453) inhibits HRV3 binding by 50% in the same assay at 240 .mu.g/ml or 3.1 .mu.M. On a molar basis the ICAM-1 IgG immunoadhesin was thus almost a 1000-fold better competitor than the monomer. The above experiments were done with supernatants. Subsequent attempts to reproduce these results with highly purified tICAM(185)/IgG were unsuccessful.
The tICAM(453)/IgG immunoadhesin of Example 12 consists of residues 1-453 of ICAM-1 fused to residue 216 in the hinge region of an IgGl heavy chain. The molecule is a disulfide-linked dimer containing two rhinovirus binding sites. The fusion polypeptide was expressed in HeLa cells using the vaccinia/T7 system and purified from the supernatant by affinity chromatography using an anti-ICAM-1 monoclonal antibody. The activity of the protein was examined in a competitive binding assay which measures the binding of [.sup.35 S]-labelled HRV to plates coated with purified tmICAM-1. For comparison, soluble monomeric tICAM-453 was included in a parallel assay as a positive control. The binding values are documented in Table 8 below:
TABLE 8______________________________________ IC.sub.50 *______________________________________ tICAM(453) 44 nM t(453)/IgG Experiment 1 11 nM Experiment 2 10 nM______________________________________ *IC.sub.50 is the concentration required to inhibit binding by 50%
These values are per mol of tICAM(453) determined by RIA. Since each fusion polypeptide contains two tICAM(453) polypeptides, the values for the fusion polypeptide expressed per mol of dimer are 5.5 nM and 5 nM for Experiments 1 and 2, respectively. Therefore on a molar basis the activity of the fusion polypeptide in the competitive binding assay is ten-fold greater than the tICAM(453) monomer. In subsequent experiments the relative activity was 2- to 4-fold greater.
EXAMPLE 14
IN VITRO DIMERIZATION OF ICAM-1
Several lines of evidence indicate that tmICAM-1 exists as a noncovalent dimer at the cell surface: (i) the stoichiometry of HRV/ICAM-1 binding sites at the cell surface is approximately 2; (ii) tICAM(453), despite being properly folded, has a approximately 100-fold lower affinity for HRV than purified tmICAM-1; and (iii) tICAM(453) and tmICAM-1 absorbed to nitrocellulose filters at a high density bind rhinovirus at equivalent levels. See Example 7. In addition, Staunton et al. (Cell 61:243-254 (1990)) have reported that some mutants of ICAM-1 form covalent dimers at the cell surface, indicating that this protein has the capability to self-associate in vivo. Attempts to directly demonstrate the existence of dimers by chemical cross-linking with water-soluble carbodiimide/NHS, which is a heterobifunctional crosslinker which forms a covalent bond between a primary amine and a carboxyl group, did result in crosslinking of tICAM(453) into a 180 kD species, whose size is consistent with a dimer (FIG. 3A). This crosslinking is directly dependent upon the concentration of tICAM(453), with 50% crosslinking at 7 .mu.M protein (FIG. 3B). This concentration is consistent with the relatively high concentration of tmICAM-1 at the surface of a HeLa cell, which is approximately 2.5 .mu.M or 135 .mu.g/ml. The self-association detected by this crosslinking is specific, since it is not affected by high concentrations of third-party proteins (FIG. 3C). tICAM(185) appears to be poorly crosslinked under the same conditions, indicating that domains 3-5 are involved in self-association. Because of the extensive modification of the protein by this crosslinking procedure, the protein had no virus-binding activity. However, this data shows that soluble ICAM can self-associate in solution, and that this self-association is concentration-dependent and -specific.
EXAMPLE 15
A tICAM(1-451)/LFA-3(210-237) CHIMERA
In order to examine the role of the transmembrane and cytoplasmic domains of tmICAM-1 in high-affinity rhinovirus binding, we constructed a chimeric ICAM-1 which is anchored on the cell surface by a phospholipid tail and lacks these domains (see FIG. 4). This experiment was designed to test whether the cytoplasmic and transmembrane domains are necessary for the formation of dimeric ICAM-1 on the cell surface, which results in the high affinity binding of rhinovirus. In order to modify the ICAM-1 cDNA to express a phospholipid-anchored form, we first used site-directed mutagenesis to create a unique SacII site at residues 450/451 close to the end of the extracellular region. This allowed the isolation of a cDNA fragment coding for residues 1-451 of ICAM-1, by digestion of the modified plasmid with HindIII and SacII. We used PCR to generate a fragment coding for the C-terminal 28 amino acids of the phospholipid-anchored form of LFA-3 (Seed, B., Nature (1987) 329:840-842). By including a SacII site in the 5' primer this fragment was ligated to the ICAM-1 extracellular domain and cloned into the expression vector CDM8, resulting in the plasmid pHRR 70-19. This plasmid contains a cDNA coding for residues 1-451 of ICAM-1 fused to residues 210-237 of LFA-1, which should result in the expression of a phosphoplipid-anchored molecule containing the ICAM-1 extracellular region. See FIG. 4.
Transfection of COS cells with pHRR 70-19 according to the method of Example 4 and FACS analysis with anti-ICAM-1 antibodies confirmed the cell surface expression of the fusion protein. The binding of [.sup.35 S]-labelled cells to COS cells transfected with the fusion protein was determined.
TABLE 9______________________________________ICAM-1 cpm bound % virus input % control______________________________________tmICAM-1 2130 +/- 278 9.4 100tICAM(1-185)/ 2382 +/- 293 11.2 119LFA-3 (210-237) chimera______________________________________
This result shows that there is no significant difference between the ability of tmICAM-1 and the tICAM(1-451)/LFA-3(210-237) chimera to bind HRV. It can therefore be concluded that the transmembrane and cytoplasmic domains are not required for HRV binding, and that dimerization must depend on interactions between extracellular regions of the molecule.
Additional evidence that a form of ICAM-1 lacking the cytoplasmic and transmembrane domains functions efficiently as a receptor for rhinoviruses was obtained by transfection of the tICAM(1-451)/LFA-3(210-237) chimeric gene into HeLa 229 cells. We have determined that these cells do not express ICAM-1 on the surface and are resistant to HRV infection. Transfection of either tmICAM-1 or the tICAM(1-451)/LFA-3(210-237) chimera results in cells which are readily infectable with rhinovirus and produce virus at levels comparable to normal HeLa cells.
EXAMPLE 16
IRREVERSIBLE INACTIVATION OF HRV BY ICAM
We have demonstrated that tICAM(453) can, in addition to blocking the binding of HRV to cells, irreversibly inactivate HRV. Incubation of HRV with tICAM(453) at 34 C results in conversion of a fraction of the virus from the native 148S form to a 42S form (FIG. 5). The 42S form is non-infectious, lacks the viral subunit VP4, and lacks the RNA genome (empty capsid). This can be shown by SDS-PAGE analysis of [.sup.35 S]methionine-labelled viral particles and by quantitation of viral RNA content by hybridization with a [.sup.32 P]oligonucleotide probe for rhinovirus (5'-GCATTCAGGGGCCGGAG-3') (SEQ ID NO:16). Thus, tICAM(453) can uncoat rhinovirus, an event that normally occurs intracellularly during the course of infection. The uncoating is a slow process, occurring with a t1/2 of 6 hours at 34 C, in contrast with the inhibition of binding, which occurs with a t1/2 of <30 minutes. The uncoating is highly temperature-dependent, occurring 10 times faster at 37 C than at 34 C, the optimal temperature of rhinovirus growth. Enhancement of this uncoating activity by soluble forms of ICAM-1 including multimeric configurations of ICAM-1 will lead to improvement of antiviral activity by making neutralization irreversible.
Example 17
CYSTEINE MUTEINS
To identify the correct site to place cysteine residues for multimerization of ICAM-1, the region of the protein surface involved in self-association must be identified. Domains IV and V have been chosen because they are distal to the viral binding sites (domain I) and because domains II-V are implicated in self-association (see Example 14). Since the structure of ICAM-1 is not certain, we have attempted to align the sequence of domains IV and V at the C-terminus of the extracellular domain of ICAM-1 onto the immunoglobulin fold, as ICAM-1 has homology to numbers of the immunoglobulin supergene family. This alignment is shown diagrammatically in FIG. 6. Then, to identify probable sites involved in self-association, we have examined the three-dimensional structures of several members of the immunoglobulin supergene family, IgG and MHC1/beta-2 microglobulin. Immunoglobulin domains have two broad faces of beta sheet structure, here designated the "B" face and the "F" face. Inspection of the above structures revealed that different immunoglobulin-like domains interacted via one or the other of these faces of the domain. IgG variable regions associated via their F face, while IgG constant regions (CH1, CH2, and CH3) and MHC1/beta-2 microglobulin all interact via their B faces.
ICAM-1 domains have highest homology to constant region-like domains. Thus, the most likely sites of interaction are on the B face of the domains; the most likely sites on the B face to place cysteine residues are close to the center of the B face (adjacent to the cysteine on the B strand that forms the intrachain disulfide bond), where IgG CH3 domains self-associate, or on the N-terminal end of the B face, where IgG CH2 domains and MHC1/beta-2 microglobulin self-associate.
A number of mutants were prepared to identify appropriate sites of interaction. These mutants were prepared by standard site-directed mutagenesis methodology to mutate selected residues to cysteine on tICAM(453) and tmICAM. These cDNAs in the vector CDM8 were then transfected into COS cells and dimer formation accessed by biosynthetic labelling of ICAM-1 with [.sup.35 S]cysteine followed by immunoprecipitation and non-reducing SDS-PAGE analysis. As shown in Table 10, of 13 mutants tested, two have been found to form dimers at a small (about 5%) but significant level:
TABLE 10______________________________________Position of Cysteine Dimer Formation______________________________________(tmICAM-1)304 -306 -307 +309 +375 -377 -378 -380 -382 -429 -(tICAM(453))338 -360 -378 -______________________________________
These two muteins, Cys-307 and Cys-309, are both located on the N-terminal end of the B face of domain IV. The relatively low level of dimerization may reflect the low concentration of ICAM-1 on the cell surface (low expression), or imperfect orientation of the cysteine residues relative to the site of interaction. These data indicate that this region of the domain is a likely site of interaction. Other residues adjacent to residues 307 and 309, e.g. His-308, Arg-310, Glu-294, Arg-326, Gln-328, are likely to increase the efficiency of the dimer formation. Mutations that lead to dimer formation of tmICAM-1 are then be placed on tICAM(453) for the secretion of soluble ICAM-1 dimers.
A tICAM(452) cysteine mutant was prepared by substituting a cysteine for an alanine at position 307 in the ICAM-1 amino acid sequence and inserting a stop codon after amino acid residue 452. The mutein was constructed by site-directed mutagenesis using a full-length ICAM-1 cDNA and has the following DNA sequence:
CAGACATCTG TGTCCCCCTC AAAAGTCATC CTGCCCCGGG GAGGCTCCGT (SEQ ID NO: 17)51 GCTGGTGACA TGCAGCACCT CCTGTGACCA GCCCAAGTTG TTGGGCATAG101 AGACCCCGTT GCCTAAAAAG GAGTTGCTCC TGCCTGGGAA CAACCGGAAG151 GTGTATGAAC TGAGCAATGT GCAAGAAGAT AGCCAACCAA TGTGCTATTC201 AAACTGCCCT GATGGGCAGT CAACAGCTAA AACCTTCCTC ACCGTGTACT251 GGACTCCAGA ACGGGTGGAA CTGGCACCCC TCCCCTCTTG GCAGCCAGTG301 GGCAAGAACC TTACCCTACG CTGCCAGGTG GAGGGTGGGG CACCCCGGGC351 CAACCTCACC GTGGTGCTGC TCCGTGGGGA GAAGGAGCTG AAACGGGAGC401 CAGCTGTGGG GGAGCCCGCT GAGGTCACGA CCACGGTGCT GGTGAGGAGA451 GATCACCATG GAGCCAATTT CTCGTGCCGC ACTGAACTGG ACCTGCGGCC501 CCAAGGGCTG GAGCTGTTTG AGAACACCTC GGCCCCCTAC CAGCTCCAGA551 CCTTTGTCCT GCCAGCGACT CCCCCACAAC TTGTCAGCCC CCGGGTCCTA601 GAGGTGGACA CGCAGGGGAC CGTGGTCTGT TCCCTGGACG GGCTGTTCCC651 AGTCTCGGAG GCCCAGGTCC ACCTGGCACT GGGGGACCAG AGGTTGAACC701 CCACAGTCAC CTATGGCAAC GACTCCTTCT CGGCCAAGGC CTCAGTCAGT751 GTGACCGCAG AGGACGAGGG CACCCAGCGG CTGACGTGTG CAGTAATACT801 GGGGAACCAG AGCCAGGAGA CACTGCAGAC AGTGACCATC TACAGCTTTC851 CGGCGCCCAA CGTGATTCTG ACGAAGCCAG AGGTCTCAGA AGGGACCGAG901 GTGACAGTGA AGTGTGAGtg CCACccgcgg GCCAAGGTGA CGCTGAATGG951 GGTTCCAGCC CAGCCACTGG GCCCGAGGGC CCAGCTCCTG CTGAAGGCCA1001 CCCCAGAGGA CAACGGGCGC AGCTTCTCCT GCTCTGCAAC CCTGGAGGTG1051 GCCGGCCAGC TTATACACAA GAACCAGACC CGGGAGCTTC GTGTCCTGTA1101 TGGCCCCCGA CTGGACGAGA GGGATTGTCC GGGAAACTGG ACGTGGCCAG1151 AAAATTCCCA GCAGACTCCA ATGTGCCAGG CTTGGGGGAA CCCATTGCCC1201 GAGCTCAAGT GTCTAAAGGA TGGCACTTTC CCACTGCCCA TCGGGGAATC1251 AGTGACTGTC ACTCGAGATC TTGAGGGCAC CTACCTCTGT CGGGCCAGGA1301 GCACTCAAGG GGAGGTCACC CGCAAGGTGA CCGTGAATGT GCTCTCCCCC1351 CGGTATTAG
The foregoing examples describe the creation of soluble, multimeric forms of tICAM that substantially increase tICAM binding and neutralizing activity.
While the present invention has been described in terms of specific methods and compositions, it is understood that variations and modifications will occur to those skilled in the art upon consideration of the present invention.
For example, it is anticipated that smaller protein fragments and peptides derived from ICAM-1 that still contain the virus-binding site would also be effective in a multimeric configuration. It is also anticipated that multimeric ICAM may be effective inhibitors of the ICAM-1/LFA-1 interaction, as the affinity between these two molecules is quite low and the cell-cell binding mediated by these two molecules is highly cooperative.
Although the preferred form and configuration is a non-transmembrane (truncated) ICAM in dimeric configuration, it is not intended to preclude other forms and configurations effective in binding virus and effective in neutralizing viral activity from being included in the scope of the present invention.
Further, it is anticipated that the general method of the invention of preparing soluble protein forms from insoluble, normally membrane bound receptor proteins can be used to prepare soluble multimeric forms of other receptor proteins useful for binding to and decreasing infectivity of viruses other than those that bind to the "major group" receptor. Such other viruses include polio, Herpes simplex, and Epstein-Barr virus.
Numerous modifications and variations in the invention as described in the above illustrative examples are expected to occur to those skilled in the art and consequently only such limitations as appear in the appended claims should be placed thereon.
Accordingly it is intended in the appended claims to cover all such equivalent variations which come within the scope of the invention as claimed.
__________________________________________________________________________# SEQUENCE LISTING- (1) GENERAL INFORMATION:- (iii) NUMBER OF SEQUENCES: 20- (2) INFORMATION FOR SEQ ID NO:1:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 31 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: no- (ix) FEATURE:#(5' PCR primer)ME/KEY: PCR 5.1 (B) LOCATION: 5' end - # of ICAM-1 coding sequence#bp 1 = G; bp 2-7 = EcoRI site;:#= 24 bases coding for the first eight amino aci - #d residues of hICAM-1- (x) PUBLICATION INFORMATION: (A) AUTHORS: Greve, J.M - #., G. Davis, A.M. Meyer, C.P. Forte, S. - #C. Yost, C.W. Marlor, M.E. Kamarck, and A. McClel - #land#Human Rhinovirus Receptor isr ICAM-1 (C) JOURNAL: Cell (D) VOLUME: 56 (F) PAGES: 839-847#1989 (G) DATE: March 10, (K) RELEVANT RESIDUES I - #N SEQ ID NO:1: From 1 to 31- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:# 31 CC AGC AGC CCC CGG CCC#Arg ProMet Ala Pro Ser Ser Pro# 5- (2) INFORMATION FOR SEQ ID NO:2:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 31 bp (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: yes- (ix) FEATURE:#(3' PCR primer)ME/KEY: PCR 3.1 (B) LOCATION: 3' end - # of ICAM-1 coding sequence#bp 1 = G; bp 2-7 = EcoRI site;:#= 24 bases complementary to nucleic acid#coding for last 8 amino acid residues of hICAM-1- (x) PUBLICATION INFORMATION: (A) AUTHORS: Greve, J.M - #., G. Davis, A.M. Meyer, C.P. Forte, S. - #C. Yost, C.W. Marlor, M.E. Kamarck, and A. McClel - #land#Human Rhinovirus Receptor isr ICAM-1 (C) JOURNAL: Cell (D) VOLUME: 56 (F) PAGES: 839-847#1989 (G) DATE: March 10, (K) RELEVANT RESIDUES I - #N SEQ ID NO:2: From 1 to 31- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:# 31 CGTG GCTTGTGTGT T- (2) INFORMATION FOR SEQ ID NO:3:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 47 bp (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: yes- (ix) FEATURE: (A) NAME/KEY: ICAM1 pro - #be#complementary to nucleotidesON:#611 of ICAM-1 565 to- (x) PUBLICATION INFORMATION: (A) AUTHORS: Greve, J.M - #., G. Davis, A.M. Meyer, C.P. Forte, S. - #C. Yost, C.W. Marlor, M.E. Kamarck, and A. McClel - #land#Human Rhinovirus Receptor isr ICAM-1 (C) JOURNAL: Cell (D) VOLUME: 56 (F) PAGES: 839-847#1989 (G) DATE: March 10, (K) RELEVANT RESIDUES I - #N SEQ ID NO:3: From 1 to 47- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:# 47GCTC CAGCCCTTGG GGCCGCAGGT CCAGTTC- (2) INFORMATION FOR SEQ ID NO:4:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 47 bp (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: yes- (ix) FEATURE: (A) NAME/KEY: ICAM3 pro - #be (B) LOCATION: complementar - #y to nucleotides 659 to 705#ICAM-1 of human- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:# 47GTCT GGAGCTGGTA GGGGGCCGAG GTGTTCT- (2) INFORMATION FOR SEQ ID NO:5:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 49 bp (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: no- (ix) FEATURE: (A) NAME/KEY: PCR 5.5#bp 1 = G; bp 2-7 = EcoRI site;:#= HindIII site; bp 14-25 = hICAM-1 5' untranslated - # region; bp 26-49 = sequence coding for first - # 8 amino acid residues of hICAM-1- (x) PUBLICATION INFORMATION: (A) AUTHORS: Greve, J.M - #., C.P. Forte, C.W. Marlor, A.M. Meye - #r, H. Hoover-Litty, D. Wunderlich, and A. McClelland#of Receptor-mediated Rhinovirus Neutralizati - #on Defined by Two Soluble Forms of ICAM-1" (C) JOURNAL: Journal of - # Virology (D) VOLUME: 65 (E) ISSUE: 11 (F) PAGES: 6015-6023 (G) DATE: 1991 (K) RELEVANT RESIDUES I - #N SEQ ID NO:5: From 1 to 49- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:#CCC CGG CCC 49CGCT ATG GCT CCC AGC AGC# Met Ala Pro Ser Ser - # Pro Arg Pro# 5- (2) INFORMATION FOR SEQ ID NO:6:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 40 bp (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: yes- (ix) FEATURE: (A) NAME/KEY: PCR 3.3#bp 1 = G; bp 2-7 = EcoRI site;:#17-stI site; bp 14-16 = stop codon; bp#complementary to nucleotide sequence coding for resid - #ues 453-446 of hICAM-1- (x) PUBLICATION INFORMATION: (A) AUTHORS: Greve, J.M - #., C.P. Forte, C.W. Marlor, A.M. Meye - #r, H. Hoover-Litty, D. Wunderlich, and A. McClelland (B) TITLE: Mechanisms o - #f Receptor-mediated Rhinovirus Neutralizati - #on Defined by Two Soluble Forms of ICAM-1 (C) JOURNAL: Journal of - # Virology (D) VOLUME: 65 (E) ISSUE: 11 (F) PAGES: 6015-6023 (G) DATE: 1991 (K) RELEVANT RESIDUES I - #N SEQ ID NO:6: From 1 to 40- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:# 40 CTCA TACCGGGGGG AGAGCACATT- (2) INFORMATION FOR SEQ ID NO:7:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 39 bp (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: yes- (ix) FEATURE: (A) NAME/KEY: PCR 3.10#bp 1 = T; bp 2-7 = Xba site; bp# Bam site; bp 14-16 = stop codon; bp 17-3 - #9 =#complementary to nucleotides 693-671 which cod - #e for amino acid residues 185-178 of hICAM-1- (x) PUBLICATION INFORMATION: (A) AUTHORS: Greve, J.M - #., C.P. Forte, C.W. Marlor, A.M. Meye - #r, H. Hoover-Litty, D. Wunderlich, and A. McClelland (B) TITLE: Mechanisms o - #f Receptor-mediated Rhinovirus Neutralizati - #on Defined by Two Soluble Forms of ICAM-1 (C) JOURNAL: Journal of - # Virology (D) VOLUME: 65 (E) ISSUE: 11 (F) PAGES: 6015-6023 (G) DATE: 1991 (K) RELEVANT RESIDUES I - #N SEQ ID NO:7: From 1 to 39- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:# 39 AAAG GTCTGGAGCT GGTAGGGGG- (2) INFORMATION FOR SEQ ID NO:8:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 43 bp (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: yes- (ix) FEATURE: (A) NAME/KEY: PCR 3.19#bp 1 = T; bp 2-7 = Xba site; bp# Bam site; bp 14-16 = stop codon; bp 17-1 - #9 = 43 = sequence complementarydon; bp 20 to nucleo - #tides coding for amino acid residues 453-#hICAM-1 446 of- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:# 43 CTTC TCATACCGGG GGGAGAGCAC ATT- (2) INFORMATION FOR SEQ ID NO:9:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 43 bp (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: yes- (ix) FEATURE: (A) NAME/KEY: PCR 3.20#bp 1 = T; bp 2-7 = Xba site; bp# Bam site; bp 14-16 = stop codon; bp 17-1 - #9 = 43 = sequence complementarydon; bp 20 to nucleo - #tides coding for amino acid residues 185-#hICAM-1 178 of- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9:# 43 CTTA AAGGTCTGGA GCTGGTAGGG GGC- (2) INFORMATION FOR SEQ ID NO:10:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 48 bp (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: yes- (ix) FEATURE: (A) NAME/KEY: 3' PCR - # primer for tICAM(185)/IgG chimera#bp 1-24 = complementary toTION:#coding for amino acid residues 216-223#IgG1 heavy chain; bp 25-48 = complementar - #y to last 24 bases of nucleotide#coding for tICAM(185)e- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:# 48GTTT TGTCAAAGGT CTGGAGCTGG TAGGGGGC- (2) INFORMATION FOR SEQ ID NO:11:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 bp (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: no- (ix) FEATURE: (A) NAME/KEY: 5' PCR - # primer for IgG fragment#coding for residues 216-223 of: human IgG - #1 heavy chain- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:# 24CA TGC CCA CGGAsp Lys Thr His Thr Ser Pro Arg#5- (2) INFORMATION FOR SEQ ID NO:12:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 40 bp (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: yes- (ix) FEATURE: (A) NAME/KEY: 3' PCR - # primer for IgG fragment#bp 1 = G; bp 2-7 = Bam site; bp# Xba site; bp 14-16 = stop codon; bp 17-4 - #0 =#complementary to last 8 residues of human IgG1 heav - #y chain- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:# 40 TTTA CCCGGAGACA GGGAGAGGCT- (2) INFORMATION FOR SEQ ID NO:13:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 48 bp (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: yes- (ix) FEATURE: (A) NAME/KEY: 3' PCR - # primer for the construction of tICAM(453)/I - #gG chimera#bp 1-24 = complementary toTION:#coding for amino acid residues 216-223#IgG1 heavy chain; bp 25-48 = complementar - #y to nucleotide sequence coding for amino aci - #d residues 446-453 of tICAM(453)- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:# 48GTTT TGTCCTCATA CCGGGGGGAG AGCACATT- (2) INFORMATION FOR SEQ ID NO:14:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2043 bp (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: no- (ix) FEATURE: (A) NAME/KEY: tICAM(453)/I - #gG fusion#bp 1-1359 = nucleotides coding: for amino - # acid residues 1-453 of ICAM-1; bp 1360-# nucleotides coding for amino acid residues 216-442 o - #f human heavy chain IgG1; bp 2401-2043 = stop codo - #n- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14:- CAG ACA TCT GTG TCC CCC TCA AAA GTC ATC CT - #G CCC CGG GGA GGC#45Gln Thr Ser Val Ser Pro Ser Lys Val Ile Le - #u Pro Arg Gly Gly#15- TCC GTG CTG GTG ACA TGC AGC ACC TCC TGT GA - #C CAG CCC AAG TTG#90Ser Val Leu Val Thr Cys Ser Thr Ser Cys As - #p Gln Pro Lys Leu# 30- TTG GGC ATA GAG ACC CCG TTG CCT AAA AAG GA - #G TTG CTC CTG CCT 13 - #5Leu Gly Ile Glu Thr Pro Leu Pro Lys Lys Gl - #u Leu Leu Leu Pro# 45- GGG AAC AAC CGG AAG GTG TAT GAA CTG AGC AA - #T GTG CAA GAA GAT 18 - #0Gly Asn Asn Arg Lys Val Tyr Glu Leu Ser As - #n Val Gln Glu Asp# 60- AGC CAA CCA ATG TGC TAT TCA AAC TGC CCT GA - #T GGG CAG TCA ACA 22 - #5Ser Gln Pro Met Cys Tyr Ser Asn Cys Pro As - #p Gly Gln Ser Thr# 75- GCT AAA ACC TTC CTC ACC GTG TAC TGG ACT CC - #A GAA CGG GTG GAA 27 - #0Ala Lys Thr Phe Leu Thr Val Tyr Trp Thr Pr - #o Glu Arg Val Glu# 90- CTG GCA CCC CTC CCC TCT TGG CAG CCA GTG GG - #C AAG AAC CTT ACC 31 - #5Leu Ala Pro Leu Pro Ser Trp Gln Pro Val Gl - #y Lys Asn Leu Thr# 105- CTA CGC TGC CAG GTG GAG GGT GGG GCA CCC CG - #G GCC AAC CTC ACC 36 - #0Leu Arg Cys Gln Val Glu Gly Gly Ala Pro Ar - #g Ala Asn Leu Thr# 120- GTG GTG CTG CTC CGT GGG GAG AAG GAG CTG AA - #A CGG GAG CCA GCT 40 - #5Val Val Leu Leu Arg Gly Glu Lys Glu Leu Ly - #s Arg Glu Pro Ala# 135- GTG GGG GAG CCC GCT GAG GTC ACG ACC ACG GT - #G CTG GTG AGG AGA 45 - #0Val Gly Glu Pro Ala Glu Val Thr Thr Thr Va - #l Leu Val Arg Arg# 150- GAT CAC CAT GGA GCC AAT TTC TCG TGC CGC AC - #T GAA CTG GAC CTG 49 - #5Asp His His Gly Ala Asn Phe Ser Cys Arg Th - #r Glu Leu Asp Leu# 165- CGG CCC CAA GGG CTG GAG CTG TTT GAG AAC AC - #C TCG GCC CCC TAC 54 - #0Arg Pro Gln Gly Leu Glu Leu Phe Glu Asn Th - #r Ser Ala Pro Tyr# 180- CAG CTC CAG ACC TTT GTC CTG CCA GCG ACT CC - #C CCA CAA CTT GTC 58 - #5Gln Leu Gln Thr Phe Val Leu Pro Ala Thr Pr - #o Pro Gln Leu Val# 195- AGC CCC CGG GTC CTA GAG GTG GAC ACG CAG GG - #G ACC GTG GTC TGT 63 - #0Ser Pro Arg Val Leu Glu Val Asp Thr Gln Gl - #y Thr Val Val Cys# 210- TCC CTG GAC GGG CTG TTC CCA GTC TCG GAG GC - #C CAG GTC CAC CTG 67 - #5Ser Leu Asp Gly Leu Phe Pro Val Ser Glu Al - #a Gln Val His Leu# 225- GCA CTG GGG GAC CAG AGG TTG AAC CCC ACA GT - #C ACC TAT GGC AAC 72 - #0Ala Leu Gly Asp Gln Arg Leu Asn Pro Thr Va - #l Thr Tyr Gly Asn# 240- GAC TCC TTC TCG GCC AAG GCC TCA GTC AGT GT - #G ACC GCA GAG GAC 76 - #5Asp Ser Phe Ser Ala Lys Ala Ser Val Ser Va - #l Thr Ala Glu Asp# 255- GAG GGC ACC CAG CGG CTG ACG TGT GCA GTA AT - #A CTG GGG AAC CAG 81 - #0Glu Gly Thr Gln Arg Leu Thr Cys Ala Val Il - #e Leu Gly Asn Gln# 270- AGC CAG GAG ACA CTG CAG ACA GTG ACC ATC TA - #C AGC TTT CCG GCG 85 - #5Ser Gln Glu Thr Leu Gln Thr Val Thr Ile Ty - #r Ser Phe Pro Ala# 285- CCC AAC GTG ATT CTG ACG AAG CCA GAG GTC TC - #A GAA GGG ACC GAG 90 - #0Pro Asn Val Ile Leu Thr Lys Pro Glu Val Se - #r Glu Gly Thr Glu# 300- GTG ACA GTG AAG TGT GAG GCC CAC CCT AGA GC - #C AAG GTG ACG CTG 94 - #5Val Thr Val Lys Cys Glu Ala His Pro Arg Al - #a Lys Val Thr Leu# 315- AAT GGG GTT CCA GCC CAG CCA CTG GGC CCG AG - #G GCC CAG CTC CTG 99 - #0Asn Gly Val Pro Ala Gln Pro Leu Gly Pro Ar - #g Ala Gln Leu Leu# 330- CTG AAG GCC ACC CCA GAG GAC AAC GGG CGC AG - #C TTC TCC TGC TCT1035Leu Lys Ala Thr Pro Glu Asp Asn Gly Arg Se - #r Phe Ser Cys Ser# 345- GCA ACC CTG GAG GTG GCC GGC CAG CTT ATA CA - #C AAG AAC CAG ACC1080Ala Thr Leu Glu Val Ala Gly Gln Leu Ile Hi - #s Lys Asn Gln Thr# 360- CGG GAG CTT CGT GTC CTG TAT GGC CCC CGA CT - #G GAC GAG AGG GAT1125Arg Glu Leu Arg Val Leu Tyr Gly Pro Arg Le - #u Asp Glu Arg Asp# 375- TGT CCG GGA AAC TGG ACG TGG CCA GAA AAT TC - #C CAG CAG ACT CCA1170Cys Pro Gly Asn Trp Thr Trp Pro Glu Asn Se - #r Gln Gln Thr Pro# 390- ATG TGC CAG GCT TGG GGG AAC CCA TTG CCC GA - #G CTC AAG TGT CTA1215Met Cys Gln Ala Trp Gly Asn Pro Leu Pro Gl - #u Leu Lys Cys Leu# 405- AAG GAT GGC ACT TTC CCA CTG CCC ATC GGG GA - #A TCA GTG ACT GTC1260Lys Asp Gly Thr Phe Pro Leu Pro Ile Gly Gl - #u Ser Val Thr Val# 420- ACT CGA GAT CTT GAG GGC ACC TAC CTC TGT CG - #G GCC AGG AGC ACT1305Thr Arg Asp Leu Glu Gly Thr Tyr Leu Cys Ar - #g Ala Arg Ser Thr# 435- CAA GGG GAG GTC ACC CGC AAG GTG ACC GTG AA - #T GTG CTC TCC CCC1350Gln Gly Glu Val Thr Arg Lys Val Thr Val As - #n Val Leu Ser Pro# 450- CGG TAT GAG GAC AAA ACT CAC ACA TGC CCA CC - #G TGC CCA GCA CCT1395Arg Tyr Glu Asp Lys Thr His Thr Cys Pro Pr - #o Cys Pro Ala Pro# 465- GAA CTC CTG GGG GGA CCG TCA GTC TTC CTC TT - #C CCC CCA AAA CCC1440Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Ph - #e Pro Pro Lys Pro# 480- AAG GAC ACC CTC ATG ATC TCC CGG ACC CCT GA - #G GTC ACA TGC GTG1485Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Gl - #u Val Thr Cys Val# 495- GTG GTG GAC GTG AGC CAC GAA GAC CCT GAG GT - #C AAG TTC AAC TGG1530Val Val Asp Val Ser His Glu Asp Pro Glu Va - #l Lys Phe Asn Trp# 510- TAC GTG GAC GGC GTG GAG GTG CAT AAT GCC AA - #G ACA AAG CCG CGG1575Tyr Val Asp Gly Val Glu Val His Asn Ala Ly - #s Thr Lys Pro Arg# 525- GAG GAG CAG TAC AAC AGC ACG TAC CGG GTG GT - #C AGC GTC CTC ACC1620Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Va - #l Ser Val Leu Thr# 540- GTC CTG CAC CAG GAC TGG CTG AAT GGC AAG GA - #G TAC AAG TGC AAG1665Val Leu His Gln Asp Trp Leu Asn Gly Lys Gl - #u Tyr Lys Cys Lys# 555- GTC TCC AAC AAA GCC CTC CCA GCC CCC ATC GA - #G AAA ACC ATC TCC1710Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Gl - #u Lys Thr Ile Ser# 570- AAA GCC AAA GGG CAG CCC CGA GAA CCA CAG GT - #G TAC ACC CTG CCC1755Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Va - #l Tyr Thr Leu Pro# 585- CCA TCC CGG GAT GAG CTG ACC AAG AAC CAG GT - #C AGC CTG ACC TGC1800Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Va - #l Ser Leu Thr Cys# 600- CTG GTC AAA GGC TTC TAT CCC AGC GAC ATC GC - #C GTG GAG TGG GAG1845Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Al - #a Val Glu Trp Glu# 615- AGC AAT GGG CAG CCG GAG AAC AAC TAC AAG AC - #C ACG CCT CCC GTG1890Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Th - #r Thr Pro Pro Val# 630- CTG GAC TCC GAC GGC TCC TTC TTC CTC TAC AG - #C AAG CTC ACC GTG1935Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Se - #r Lys Leu Thr Val# 645- GAC AAG AGC AGG TGG CAG CAG GGG AAC GTC TT - #C TCA TGC TCC GTG1980Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Ph - #e Ser Cys Ser Val# 660- ATG CAT GAG GCT CTG CAC AAC CAC TAC ACG CA - #G AAG AGC CTC TCC2025Met His Glu Ala Leu His Asn His Tyr Thr Gl - #n Lys Ser Leu Ser# 675#2043 AA TGALeu Ser Pro Gly Lys 680- (2) INFORMATION FOR SEQ ID NO:15:- (i) SEQUENCE CHARACTERISTICS:#acid residuesLENGTH: 680 amino (B) TYPE: amino acids (C) TOPOLOGY: linear- (ii) MOLECULE TYPE: (A) DESCRIPTION: protein- (iii) HYPOTHETICAL: no- (v) FRAGMENT TYPE: complete sequence- (ix) FEATURE: (A) NAME/KEY: tICAM(185)/I - #gG fusion protein#amino acid residues 1-453 =ION:#amino acid residues 454-680 = amino acid resi - #dues 216-442 of human IgG1 heavy chain- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15:- Gln Thr Ser Val Ser Pro Ser Lys Val Ile Le - #u Pro Arg Gly Gly#15- Ser Val Leu Val Thr Cys Ser Thr Ser Cys As - #p Gln Pro Lys Leu# 30- Leu Gly Ile Glu Thr Pro Leu Pro Lys Lys Gl - #u Leu Leu Leu Pro# 45- Gly Asn Asn Arg Lys Val Tyr Glu Leu Ser As - #n Val Gln Glu Asp# 60- Ser Gln Pro Met Cys Tyr Ser Asn Cys Pro As - #p Gly Gln Ser Thr# 75- Ala Lys Thr Phe Leu Thr Val Tyr Trp Thr Pr - #o Glu Arg Val Glu# 90- Leu Ala Pro Leu Pro Ser Trp Gln Pro Val Gl - #y Lys Asn Leu Thr# 105- Leu Arg Cys Gln Val Glu Gly Gly Ala Pro Ar - #g Ala Asn Leu Thr# 120- Val Val Leu Leu Arg Gly Glu Lys Glu Leu Ly - #s Arg Glu Pro Ala# 135- Val Gly Glu Pro Ala Glu Val Thr Thr Thr Va - #l Leu Val Arg Arg# 150- Asp His His Gly Ala Asn Phe Ser Cys Arg Th - #r Glu Leu Asp Leu# 165- Arg Pro Gln Gly Leu Glu Leu Phe Glu Asn Th - #r Ser Ala Pro Tyr# 180- Gln Leu Gln Thr Phe Val Leu Pro Ala Thr Pr - #o Pro Gln Leu Val# 195- Ser Pro Arg Val Leu Glu Val Asp Thr Gln Gl - #y Thr Val Val Cys# 210- Ser Leu Asp Gly Leu Phe Pro Val Ser Glu Al - #a Gln Val His Leu# 225- Ala Leu Gly Asp Gln Arg Leu Asn Pro Thr Va - #l Thr Tyr Gly Asn# 240- Asp Ser Phe Ser Ala Lys Ala Ser Val Ser Va - #l Thr Ala Glu Asp# 255- Glu Gly Thr Gln Arg Leu Thr Cys Ala Val Il - #e Leu Gly Asn Gln# 270- Ser Gln Glu Thr Leu Gln Thr Val Thr Ile Ty - #r Ser Phe Pro Ala# 285- Pro Asn Val Ile Leu Thr Lys Pro Glu Val Se - #r Glu Gly Thr Glu# 300- Val Thr Val Lys Cys Glu Ala His Pro Arg Al - #a Lys Val Thr Leu# 315- Asn Gly Val Pro Ala Gln Pro Leu Gly Pro Ar - #g Ala Gln Leu Leu# 330- Leu Lys Ala Thr Pro Glu Asp Asn Gly Arg Se - #r Phe Ser Cys Ser# 345- Ala Thr Leu Glu Val Ala Gly Gln Leu Ile Hi - #s Lys Asn Gln Thr# 360- Arg Glu Leu Arg Val Leu Tyr Gly Pro Arg Le - #u Asp Glu Arg Asp# 375- Cys Pro Gly Asn Trp Thr Trp Pro Glu Asn Se - #r Gln Gln Thr Pro# 390- Met Cys Gln Ala Trp Gly Asn Pro Leu Pro Gl - #u Leu Lys Cys Leu# 405- Lys Asp Gly Thr Phe Pro Leu Pro Ile Gly Gl - #u Ser Val Thr Val# 420- Thr Arg Asp Leu Glu Gly Thr Tyr Leu Cys Ar - #g Ala Arg Ser Thr# 435- Gln Gly Glu Val Thr Arg Lys Val Thr Val As - #n Val Leu Ser Pro# 450- Arg Tyr Glu Asp Lys Thr His Thr Cys Pro Pr - #o Cys Pro Ala Pro# 465- Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Ph - #e Pro Pro Lys Pro# 480- Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Gl - #u Val Thr Cys Val# 495- Val Val Asp Val Ser His Glu Asp Pro Glu Va - #l Lys Phe Asn Trp# 510- Tyr Val Asp Gly Val Glu Val His Asn Ala Ly - #s Thr Lys Pro Arg# 525- Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Va - #l Ser Val Leu Thr# 540- Val Leu His Gln Asp Trp Leu Asn Gly Lys Gl - #u Tyr Lys Cys Lys# 555- Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Gl - #u Lys Thr Ile Ser# 570- Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Va - #l Tyr Thr Leu Pro# 585- Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Va - #l Ser Leu Thr Cys# 600- Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Al - #a Val Glu Trp Glu# 615- Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Th - #r Thr Pro Pro Val# 630- Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Se - #r Lys Leu Thr Val# 645- Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Ph - #e Ser Cys Ser Val# 660- Met His Glu Ala Leu His Asn His Tyr Thr Gl - #n Lys Ser Leu Ser# 675- Leu Ser Pro Gly Lys 680- (2) INFORMATION FOR SEQ ID NO:16:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 bp (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: yes- (ix) FEATURE: (A) NAME/KEY: probe for - # HRV#complementary to nucleotidesON: 455-471 o - #f HRV-14 genome- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16:# 17 G- (2) INFORMATION FOR SEQ ID NO:17:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1359 bp (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: no- (ix) FEATURE:#cys 307 mutantAME/KEY: tICAM(452)#bp 1-1356 = sequence coding for amino aci - #d residues 1-452 of human ICAM-1 with Cys#for Ala at position 307, and codons#and Arg at positions 309 and 310 respectively - # switched to equivalent redundant codons wh - #ich provide a restriction site; bp 1357-# stop codon 1359 =- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17:- CAG ACA TCT GTG TCC CCC TCA AAA GTC ATC CT - #G CCC CGG GGA GGC#45Gln Thr Ser Val Ser Pro Ser Lys Val Ile Le - #u Pro Arg Gly Gly#15- TCC GTG CTG GTG ACA TGC AGC ACC TCC TGT GA - #C CAG CCC AAG TTG#90Ser Val Leu Val Thr Cys Ser Thr Ser Cys As - #p Gln Pro Lys Leu# 30- TTG GGC ATA GAG ACC CCG TTG CCT AAA AAG GA - #G TTG CTC CTG CCT 13 - #5Leu Gly Ile Glu Thr Pro Leu Pro Lys Lys Gl - #u Leu Leu Leu Pro# 45- GGG AAC AAC CGG AAG GTG TAT GAA CTG AGC AA - #T GTG CAA GAA GAT 18 - #0Gly Asn Asn Arg Lys Val Tyr Glu Leu Ser As - #n Val Gln Glu Asp# 60- AGC CAA CCA ATG TGC TAT TCA AAC TGC CCT GA - #T GGG CAG TCA ACA 22 - #5Ser Gln Pro Met Cys Tyr Ser Asn Cys Pro As - #p Gly Gln Ser Thr# 75- GCT AAA ACC TTC CTC ACC GTG TAC TGG ACT CC - #A GAA CGG GTG GAA 27 - #0Ala Lys Thr Phe Leu Thr Val Tyr Trp Thr Pr - #o Glu Arg Val Glu# 90- CTG GCA CCC CTC CCC TCT TGG CAG CCA GTG GG - #C AAG AAC CTT ACC 31 - #5Leu Ala Pro Leu Pro Ser Trp Gln Pro Val Gl - #y Lys Asn Leu Thr# 105- CTA CGC TGC CAG GTG GAG GGT GGG GCA CCC CG - #G GCC AAC CTC ACC 36 - #0Leu Arg Cys Gln Val Glu Gly Gly Ala Pro Ar - #g Ala Asn Leu Thr# 120- GTG GTG CTG CTC CGT GGG GAG AAG GAG CTG AA - #A CGG GAG CCA GCT 40 - #5Val Val Leu Leu Arg Gly Glu Lys Glu Leu Ly - #s Arg Glu Pro Ala# 135- GTG GGG GAG CCC GCT GAG GTC ACG ACC ACG GT - #G CTG GTG AGG AGA 45 - #0Val Gly Glu Pro Ala Glu Val Thr Thr Thr Va - #l Leu Val Arg Arg# 150- GAT CAC CAT GGA GCC AAT TTC TCG TGC CGC AC - #T GAA CTG GAC CTG 49 - #5Asp His His Gly Ala Asn Phe Ser Cys Arg Th - #r Glu Leu Asp Leu# 165- CGG CCC CAA GGG CTG GAG CTG TTT GAG AAC AC - #C TCG GCC CCC TAC 54 - #0Arg Pro Gln Gly Leu Glu Leu Phe Glu Asn Th - #r Ser Ala Pro Tyr# 180- CAG CTC CAG ACC TTT GTC CTG CCA GCG ACT CC - #C CCA CAA CTT GTC 58 - #5Gln Leu Gln Thr Phe Val Leu Pro Ala Thr Pr - #o Pro Gln Leu Val# 195- AGC CCC CGG GTC CTA GAG GTG GAC ACG CAG GG - #G ACC GTG GTC TGT 63 - #0Ser Pro Arg Val Leu Glu Val Asp Thr Gln Gl - #y Thr Val Val Cys# 210- TCC CTG GAC GGG CTG TTC CCA GTC TCG GAG GC - #C CAG GTC CAC CTG 67 - #5Ser Leu Asp Gly Leu Phe Pro Val Ser Glu Al - #a Gln Val His Leu# 225- GCA CTG GGG GAC CAG AGG TTG AAC CCC ACA GT - #C ACC TAT GGC AAC 72 - #0Ala Leu Gly Asp Gln Arg Leu Asn Pro Thr Va - #l Thr Tyr Gly Asn# 240- GAC TCC TTC TCG GCC AAG GCC TCA GTC AGT GT - #G ACC GCA GAG GAC 76 - #5Asp Ser Phe Ser Ala Lys Ala Ser Val Ser Va - #l Thr Ala Glu Asp# 255- GAG GGC ACC CAG CGG CTG ACG TGT GCA GTA AT - #A CTG GGG AAC CAG 81 - #0Glu Gly Thr Gln Arg Leu Thr Cys Ala Val Il - #e Leu Gly Asn Gln# 270- AGC CAG GAG ACA CTG CAG ACA GTG ACC ATC TA - #C AGC TTT CCG GCG 85 - #5Ser Gln Glu Thr Leu Gln Thr Val Thr Ile Ty - #r Ser Phe Pro Ala# 285- CCC AAC GTG ATT CTG ACG AAG CCA GAG GTC TC - #A GAA GGG ACC GAG 90 - #0Pro Asn Val Ile Leu Thr Lys Pro Gly Val Se - #r Glu Gly Thr Glu# 300- GTG ACA GTG AAG TGT GAG TGC CAC CCG CGG GC - #C AAG GTG ACG CTG 94 - #5Val Thr Val Lys Cys Glu Cys His Pro Arg Al - #a Lys Val Thr Leu# 315- AAT GGG GTT CCA GCC CAG CCA CTG GGC CCG AG - #G GCC CAG CTC CTG 99 - #0Asn Gly Val Pro Ala Gln Pro Leu Gly Pro Ar - #g Ala Gln Leu Leu# 330- CTG AAG GCC ACC CCA GAG GAC AAC GGG CGC AG - #C TTC TCC TGC TCT1035Leu Lys Ala Thr Pro Glu Asp Asn Gly Arg Se - #r Phe Ser Cys Ser# 345- GCA ACC CTG GAG GTG GCC GGC CAG CTT ATA CA - #C AAG AAC CAG ACC1080Ala Thr Leu Glu Val Ala Gly Gln Leu Ile Hi - #s Lys Asn Gln Thr# 360- CGG GAG CTT CGT GTC CTG TAT GGC CCC CGA CT - #G GAC GAG AGG GAT1125Arg Glu Leu Arg Val Leu Tyr Gly Pro Arg Le - #u Asp Glu Arg Asp# 375- TGT CCG GGA AAC TGG ACG TGG CCA GAA AAT TC - #C CAG CAG ACT CCA1170Cys Pro Gly Asn Trp Thr Trp Pro Glu Asn Se - #r Gln Gln Thr Pro# 390- ATG TGC CAG GCT TGG GGG AAC CCA TTG CCC GA - #G CTC AAG TGT CTA1215Met Cys Gln Ala Trp Gly Asn Pro Leu Pro Gl - #u Leu Lys Cys Leu# 405- AAG GAT GGC ACT TTC CCA CTG CCC ATC GGG GA - #A TCA GTG ACT GTC1260Lys Asp Gly Thr Phe Pro Leu Pro Ile Gly Gl - #u Ser Val Thr Val# 420- ACT CGA GAT CTT GAG GGC ACC TAC CTC TGT CG - #G GCC AGG AGC ACT1305Thr Arg Asp Leu Glu Gly Thr Tyr Leu Cys Ar - #g Ala Arg Ser Thr# 435- CAA GGG GAG GTC ACC CGC AAG GTG ACC GTG AA - #T GTG CTC TCC CCC1350Gln Gly Glu Val Thr Arg Lys Val Thr Val As - #n Val Leu Ser Pro# 450# 1359Arg Tyr- (2) INFORMATION FOR SEQ ID NO:18:- (i) SEQUENCE CHARACTERISTICS:#acid residuesLENGTH: 147 amino (B) TYPE: amino acid (C) TOPOLOGY: linear- (ii) MOLECULE TYPE: (A) DESCRIPTION: peptide- (iii) HYPOTHETICAL: no- (v) FRAGMENT TYPE: N-terminal fragment- (ix) FEATURE: (A) NAME/KEY: domains 4 - # and 5 of tmICAM-1#amino acid sequence for domains#5 of tmICAM-1 4 and- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18:- Pro Glu Val Ser Glu Gly Thr Glu Val Thr Va - #l Lys Cys Glu Ala#15- His Pro Arg Ala Lys Val Thr Leu Asn Gly Va - #l Pro Ala Gln Pro# 30- Leu Gly Pro Arg Ala Gln Leu Leu Leu Lys Al - #a Thr Pro Glu Asp# 45- Asn Gly Arg Ser Phe Ser Cys Ser Ala Thr Le - #u Glu Val Ala Gly# 60- Gln Leu Ile His Lys Arg Val Leu Tyr Gly Pr - #o Arg Leu Asp Glu# 75- Arg Asp Cys Pro Gly Asn Trp Thr Trp Pro Gl - #u Asn Ser Gln Gln# 90- Thr Pro Met Cys Gln Ala Trp Gly Asn Pro Le - #u Pro Glu Leu Lys# 105- Cys Leu Lys Pro Gly Thr Phe Pro Leu Pro Il - #e Gly Glu Ser Val# 120- Thr Val Thr Arg Asp Leu Glu Gly Thr Tyr Le - #u Cys Arg Ala Arg# 135- Ser Thr Gln Gly Glu Val Thr Arg Glu Val Th - #r Val# 145- (2) INFORMATION FOR SEQ ID NO:19:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 505 (B) TYPE: amino acids (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (iii) HYPOTHETICAL: no- (ix) FEATURE: (A) NAME/KEY: ICAM-1 pr - #otein- (x) PUBLICATION INFORMATION:#D.E., Marlin, S.D., Stratowa, C., Dustin, M.L., (B) TITLE: Primary Stru - #cture of ICAM-1 DemonstratesInteraction betwee (C) JOURNAL: Cell (D) VOLUME: 52 (F) PAGES: 925-933#1988 (G) DATE: March 25,- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:19:- Gln Thr Ser Val Ser Pro Ser Lys Val Ile Le - #u Pro Arg Gly Gly# 15- Ser Val Leu Val Thr Cys Ser Thr Ser Cys As - #p Gln Pro Lys Leu# 30- Leu Gly Ile Glu Thr Pro Leu Pro Lys Lys Gl - #u Leu Leu Leu Pro# 45- Gly Asn Asn Arg Lys Val Tyr Glu Leu Ser As - #n Val Gln Glu Asp# 60- Ser Gln Pro Met Cys Tyr Ser Asn Cys Pro As - #p Gly Gln Ser Thr# 75- Ala Lys Thr Phe Leu Thr Val Tyr Trp Thr Pr - #o Glu Arg Val Glu# 90- Leu Ala Pro Leu Pro Ser Trp Gln Pro Val Gl - #y Lys Asn Leu Thr# 105- Leu Arg Cys Gln Val Glu Gly Gly Ala Pro Ar - #g Ala Asn Leu Thr# 120- Val Val Leu Leu Arg Gly Glu Lys Glu Leu Ly - #s Arg Glu Pro Ala# 135- Val Gly Glu Pro Ala Glu Val Thr Thr Thr Va - #l Leu Val Arg Arg# 150- Asp His His Gly Ala Asn Phe Ser Cys Arg Th - #r Glu Leu Asp Leu# 165- Arg Pro Gln Gly Leu Glu Leu Phe Glu Asn Th - #r Ser Ala Pro Tyr# 180- Gln Leu Gln Thr Phe Val Leu Pro Ala Thr Pr - #o Pro Gln Leu Val# 195- Ser Pro Arg Val Leu Glu Val Asp Thr Gln Gl - #y Thr Val Val Cys# 210- Ser Leu Asp Gly Leu Phe Pro Val Ser Glu Al - #a Gln Val His Leu# 225- Ala Leu Gly Asp Gln Arg Leu Asn Pro Thr Va - #l Thr Tyr Gly Asn# 240- Asp Ser Phe Ser Ala Lys Ala Ser Val Ser Va - #l Thr Ala Glu Asp# 255- Glu Gly Thr Gln Arg Leu Thr Cys Ala Val Il - #e Leu Gly Asn Gln# 270- Ser Gln Glu Thr Leu Gln Thr Val Thr Ile Ty - #r Ser Phe Pro Ala# 285- Pro Asn Val Ile Leu Thr Lys Pro Glu Val Se - #r Glu Gly Thr Glu# 300- Val Thr Val Lys Cys Glu Ala His Pro Arg Al - #a Lys Val Thr Leu# 315- Asn Gly Val Pro Ala Gln Pro Leu Gly Pro Ar - #g Ala Gln Leu Leu# 330- Leu Lys Ala Thr Pro Glu Asp Asn Gly Arg Se - #r Phe Ser Cys Ser# 345- Ala Thr Leu Glu Val Ala Gly Gln Leu Ile Hi - #s Lys Asn Gln Thr# 360- Arg Glu Leu Arg Val Leu Tyr Gly Pro Arg Le - #u Asp Glu Arg Asp# 375- Cys Pro Gly Asn Trp Thr Trp Pro Glu Asn Se - #r Gln Gln Thr Pro# 390- Met Cys Gln Ala Trp Gly Asn Pro Leu Pro Gl - #u Leu Lys Cys Leu# 405- Lys Asp Gly Thr Phe Pro Leu Pro Ile Gly Gl - #u Ser Val Thr Val# 420- Thr Arg Asp Leu Glu Gly Thr Tyr Leu Cys Ar - #g Ala Arg Ser Thr# 435- Gln Gly Glu Val Thr Arg Glu Val Thr Val As - #n Val Leu Ser Pro# 450- Arg Tyr Glu Ile Val Ile Ile Thr Val Val Al - #a Ala Ala Val Ile# 465- Met Gly Thr Ala Gly Leu Ser Thr Tyr Leu Ty - #r Asn Arg Gln Arg# 480- Lys Ile Lys Lys Tyr Arg Leu Gln Gln Ala Gl - #n Lys Gly Thr Pro# 495- Met Lys Pro Asn Thr Gln Ala Thr Pro Pro# 505- (2) INFORMATION FOR SEQ ID NO:20:- (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1656 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: other nucleic acid- (iii) HYPOTHETICAL: no- (iv) ANTI-SENSE: no- (ix) FEATURE: (A) NAME/KEY: ICAM-1 co - #ding sequence- (x) PUBLICATION INFORMATION:#D.E., Marlin, S.D., Stratowa, C., Dustin, M.L., (B) TITLE: Primary Stru - #cture of ICAM-1 DemonstratesInteraction betwee (C) JOURNAL: Cell (D) VOLUME: 52 (F) PAGES: 925-933#1988 (G) DATE: March 25, (K) RELEVANT RESIDUES I - #N SEQ ID NO:2: From 58 to 1653- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20:- GCGCCCCAGT CGACGCTGAG CTCCTCTGCT ACTCAGAGTT GCAACCTCAG CC - #TCGCTATG 60- GCTCCCAGCA GCCCCCGGCC CGCGCTGCCC GCACTCCTGG TCCTGCTGGG GG - #CTCTGTTA 120- CCAGGACCTG GCAATGCCCA GACATCTGTG TCCCCCTCAA AAGTCATCCT GC - #CCCGGGGA 180- GGCTCCGTGC TGGTGACATG CAGCACCTCC TGTGACCAGC CCAAGTTGTT GG - #GCATAGAG 240- ACCCCGTTGC CTAAAAAGGA GTTGCTCCTG CCTGGGAACA ACCGGAAGGT GT - #ATGAACTG 300- AGCAATGTGC AAGAAGATAG CCAACCAATG TGCTATTCAA ACTGCCCTGA TG - #GGCAGTCA 360- ACAGCTAAAA CCTTCCTCAC CGTGTACTGG ACTCCAGAAC GGGTGGAACT GG - #CACCCCTC 420- CCCTCTTGGC AGCCAGTGGG CAAGAACCTT ACCCTACGCT GCCAGGTGGA GG - #GTGGGGCA 480- CCCCGGGCCA ACCTCACCGT GGTGCTGCTC CGTGGGGAGA AGGAGCTGAA AC - #GGGAGCCA 540- GCTGTGGGGG AGCCCGCTGA GGTCACGACC ACGGTGCTGG TGAGGAGAGA TC - #ACCATGGA 600- GCCAATTTCT CGTGCCGCAC TGAACTGGAC CTGCGGCCCC AAGGGCTGGA GC - #TGTTTGAG 660- AACACCTCGG CGCCCTACCA GCTCCAGACC TTTGTCCTGC CAGCGACTCC CC - #CACAACTT 720- GTCAGCCCCC GGGTCCTAGA GGTGGACACG CAGGGGACCG TGGTCTGTTC CC - #TGGACGGG 780- CTGTTCCCAG TCTCGGAGGC CCAGGTCCAC CTGGCACTGG GGGACCAGAG GT - #TGAACCCC 840- ACAGTCACCT ATGGCAACGA CTCCTTCTCG GCCAAGGCCT CAGTCAGTGT GA - #CCGCAGAG 900- GACGAGGGCA CCCAGCGGCT GACGTGTGCA GTAATACTGG GGAACCAGAG CC - #AGGAGACA 960- CTGCAGACAG TGACCATCTA CAGCTTTCCG GCGCCCAACG TGATTCTGAC GA - #AGCCAGAG1020- GTCTCAGAAG GGACCGAGGT GACAGTGAAG TGTGAGGCCC ACCCTAGAGC CA - #AGGTGACG1080- CTGAATGGGG TTCCAGCCCA GCCACTGGGC CCGAGGGCCC AGCTCCTGCT GA - #AGGCCACC1140- CCAGAGGACA ACGGGCGCAG CTTCTCCTGC TCTGCAACCC TGGAGGTGGC CG - #GCCAGCTT1200- ATACACAAGA ACCAGACCCG GGAGCTTCGT GTCCTGTATG GCCCCCGACT GG - #ACGAGAGG1260- GATTGTCCGG GAAACTGGAC GTGGCCAGAA AATTCCCAGC AGACTCCAAT GT - #GCCAGGCT1320- TGGGGGAACC CATTGCCCGA GCTCAAGTGT CTAAAGGATG GCACTTTCCC AC - #TGCCCATC1380- GGGGAATCAG TGACTGTCAC TCGAGATCTT GAGGGCACCT ACCTCTGTCG GG - #CCAGGAGC1440- ACTCAAGGGG AGGTCACCCG CGAGGTGACC GTGAATGTGC TCTCCCCCCG GT - #ATGAGATT1500- GTCATCATCA CTGTGGTAGC AGCCGCAGTC ATAATGGGCA CTGCAGGCCT CA - #GCACGTAC1560- CTCTATAACC GCCAGCGGAA GATCAAGAAA TACAGACTAC AACAGGCCCA AA - #AAGGGACC1620# 1656 CACA AGCCACGCCT CCCTGA__________________________________________________________________________

Claims

1. A method for reducing the infection by human rhinovirus (HRV) of host cells susceptible to infection by HRV, comprising contacting the virus under conditions favorable for binding with a multimeric antiviral agent comprising two or more units wherein said units may be the same or different and are each independently selected from the group consisting of transmembrane intercellular adhesion molecule-1 (tmICAM-1) and truncated forms of intercellular adhesion molecule-1 (tICAMs), each of said units containing at least one unpaired cysteine residue at a position selected from the group consisting of 307 and 309, wherein each of said units is linked to at least one other of said units via a disulfide bridge, and wherein said multimeric antiviral agent binds to HRV and reduces infectivity thereof.
2. A method for reducing infectivity of human rhinovirus of the major receptor class, said method comprising contacting said virus under conditions favorable for binding with a multimeric antiviral agent comprising two or more units wherein said units may be the same or different and are each independently selected from the group consisting of transmembrane intercellular adhesion molecule-1 (tmICAM-1) and truncated forms of intercellular adhesion molecule-1 (tICAMs), each of said units containing at least one unpaired cysteine residue at a position selected from the group consisting of 307 and 309, wherein each of said units is linked to at least one other of said units via a disulfide bridge, and wherein said multimeric antiviral agent binds to HRV and reduces infectivity thereof.
3. A method for reducing the initiation or spread of the common cold due to human rhinovirus (HRV), said method comprising contacting said virus with a multimeric antiviral agent comprising two or more units wherein said units may be the same or different and are each independently selected from the group consisting of transmembrane intercellular adhesion molecule-1 (tmICAM-1) and truncated forms of intercellular adhesion molecule-1 (tICAMs), each of said units containing at least one unpaired cysteine residue at a position selected from the group consisting of 307 and 309, wherein each of said units is linked to at least one other of said units via a disulfide bridge, and wherein said multimeric antiviral agent binds to HRV and reduces infectivity thereof.
4. The method of claim 1, 2, or 3 wherein at least one of said units is tmICAM-1.
5. The method of claim 1, 2, or 3 wherein all of said units are tmICAM-1.
6. The method of claim 1, 2, or 3 wherein at least one of said units is tICAM(453).
7. The method of claim 1, 2, or 3 wherein all of said units are tICAM(453).

Parent Case Info

This application is a continuation of copending application U.S. Ser. No. 07/903,069, filed Jun. 22, 1992 now abandoned, which is a continuation-in-part of U.S. Ser. No. 07/704,984, filed May 24, 1991 abandoned, which is a continuation-in-part of U.S. Ser. No. 07/556,238, filed Jul. 20, 1990 abandoned.

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Continuations (1)

	Number	Date	Country
Parent	903069	Jun 1992

Continuation in Parts (2)

	Number	Date	Country
Parent	704984	May 1991
Parent	556238	Jul 1990

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