Claims
- 1. An isolated or recombinant polypeptide that is at least 70% identical to SEQ ID NO:50 over a comparison window of at least 125 contiguous amino acids, wherein at pH 5.5 said polypeptide has a fumonisin detoxification activity or a fumonisin derivative detoxification activity that is at least 1.5-fold greater than any of the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 2. The polypeptide of claim 1, wherein said polypeptide has a fumonisin detoxification activity that is at least 1.5-fold greater than any of the polypeptides corresponding ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 3. The polypeptide of claim 2, wherein the fumonisin detoxification activity comprises a fumonisin deamination reaction.
- 4. The polypeptide of claim 2, wherein said polypeptide has a fumonisin detoxification activity that is at least 20-fold greater than any of the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 5. The polypeptide of claim 2, wherein said polypeptide is at least 90% identical to SEQ ID NO:50 over a comparison window of at least 125 contiguous amino acids.
- 6. The polypeptide of claim 2, wherein said polypeptide is at least 97% identical to a sequence selected the group consisting of SEQ ID NO:26 to SEQ ID NO:50 over a comparison window of at least 125 contiguous amino acids.
- 7. The polypeptide of claim 2, wherein said polypeptide has an amino acid sequence which is substantially identical over at least 125 contiguous amino acids of any one of SEQ ID NO:26 to SEQ ID NO:50.
- 8. The polypeptide of claim 2, wherein said polypeptide comprises at least 125 contiguous amino acids of any one of SEQ ID NO:26 to SEQ ID NO:50.
- 9. The polypeptide of claim 2, wherein said polypeptide is at least 97% identical to substantially the entire length of a sequence selected from the group consisting of SEQ ID NO:26 to SEQ ID NO:50.
- 10. The polypeptide of claim 2, wherein said polypeptide is selected from the group consisting of SEQ ID NO:26 to SEQ ID NO:50.
- 11. The polypeptide of claim 2, wherein the polypeptide is encoded by a polynucleotide sequence selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO:25.
- 12. The polypeptide of claim 2, wherein said polypeptide is a fumonisin amine oxidase.
- 13. The polypeptide of claim 2, wherein the optimum pH of said fumonisin detoxification activity is lower for said polypeptide than for any of the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 14. The polypeptide of claim 2, wherein the thermostability of said fumonisin detoxification activity is higher for said polypeptide than for any of the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 15. The polypeptide of claim 2, wherein said polypeptide has increased fumonisin detoxification activity upon secretion from a eukaryotic cell relative to any of the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 16. The polypeptide of claim 15, wherein said eukaryotic cell is a plant cell.
- 17. The polypeptide of claim 15, wherein said eukaryotic cell is a fungal cell.
- 18. The polypeptide of claim 2, wherein said polypeptide comprises a leader sequence that directs the secretion of the polypeptide from a plant cell.
- 19. The polypeptide of claim 18, wherein said polypeptide leader sequence is an apoplast targeting sequence.
- 20. The polypeptide of claim 18, wherein said polypeptide leader sequence is a peroxisomal targeting sequence.
- 21. The polypeptide of claim 2, wherein the fumonisin is selected from the group consisting of: a fumonisin B1, a fumonisin B2, a fumonisin B3, a fumonisin B4, and a fumonisin C1.
- 22. The polypeptide of claim 2, wherein at pH 5.5 the kcat of the fumonisin detoxification reaction catalyzed by the polypeptide is higher than the kcat of the fumonisin detoxification reaction catalyzed by any of the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 23. The polypeptide of claim 2, wherein at pH 5.5 the fumonisin KM for the fumonisin detoxification reaction catalyzed by the polypeptide is lower than the fumonisin KM for the fumonisin detoxification reaction catalyzed by any of the polypeptides corresponding ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 24. The polypeptide of claim 2, wherein at pH 5.5 the fumonisin kcat/KM of the fumonisin detoxification reaction catalyzed by the polypeptide is higher than the fumonisin kcat/KM of the fumonisin detoxification reaction catalyzed by any of the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 25. A non-native variant of the polypeptide of claim 2, wherein one or more amino acids of the encoded polypeptide have been mutated.
- 26. The polypeptide of claim 2, further comprising a polypeptide purification subsequence.
- 27. The polypeptide of claim 2, wherein the polypeptide comprises an alanine residue at position 118, a serine residue at position 136, a phenylalanine reside at position 209, a lysine residue at position 210, an isoleucine residue at position 237, a glutamic acid residue at position 272, a proline residue at position 274, and a glutamic acid residue at position 473.
- 28. The polypeptide of claim 2, wherein the polypeptide comprises an aspartic acid residue at position 193.
- 29. The polypeptide of claim 2, wherein the polypeptide comprises an altered glycosylation site.
- 30. A polypeptide which is specifically bound by a polyclonal antisera raised against one or more antigen, the antigen comprising at least one sequence selected from SEQ ID NO:26 to SEQ ID NO:50 or fragment thereof, wherein the antisera is subtracted with one or more polypeptide corresponding to one or more of: ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 31. A polypeptide which comprises a unique subsequence in a polypeptide selected from: SEQ ID NO:26 to SEQ ID NO:50, wherein the unique subsequence is unique as compared to a polypeptide corresponding to any of: ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 32. An isolated or recombinant nucleic acid comprising a polynucleotide sequence that encodes a polypeptide that is at least 70% identical to SEQ ID NO:50 over a comparison window of at least 125 contiguous amino acids, or a complementary polynucleotide sequence thereof, wherein at pH 5.5 said polypeptide has a fumonisin detoxification activity or a fumonisin derivative detoxification activity that is at least 1.5-fold greater than any of the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011 C4, or any wild-type APAO.
- 33. The nucleic acid of claim 32, wherein said polypeptide has a fumonisin detoxification activity that is at least 1.5-fold greater than any of the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 34. The nucleic acid of claim 33, wherein the fumonisin detoxification activity comprises a fumonisin deamination reaction.
- 35. The nucleic acid of claim 33, wherein said polypeptide has a fumonisin detoxification activity that is at least 20-fold greater than any of the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 36. The nucleic acid of claim 33, wherein said polypeptide is at least 90% identical to SEQ ID NO:50 over a comparison window of at least 125 contiguous amino acids.
- 37. The nucleic acid of claim 33, wherein said polypeptide is at least 97% identical to a sequence selected the group consisting of SEQ ID NO:26 to SEQ ID NO:50 over a comparison window of at least 125 contiguous amino acids.
- 38. The nucleic acid of claim 33, wherein said polypeptide has an amino acid sequence which is substantially identical over at least 125 contiguous amino acids of any one of SEQ ID NO:26 to SEQ ID NO:50.
- 39. The nucleic acid of claim 33, wherein said polypeptide comprises at least 125 contiguous amino acids of any one of SEQ ID NO:26 to SEQ ID NO:50.
- 40. The nucleic acid of claim 33, wherein said polypeptide is at least 97% identical to substantially the entire length of a sequence selected the group consisting of SEQ ID NO:26 to SEQ ID NO:50.
- 41. The nucleic acid of claim 33, wherein said polypeptide is selected from the group consisting of SEQ ID NO:26 to SEQ ID NO:50.
- 42. The nucleic acid of claim 33, wherein said polynucleotide sequence is selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO:25.
- 43. An isolated or recombinant nucleic acid comprising a polynucleotide sequence that encodes a polypeptide that at pH 5.5 has a fumonisin detoxification activity or a fumonisin derivative detoxification activity that is at least 1.5-fold greater than any of the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO, or a complementary polynucleotide sequence thereof, wherein said polynucleotide sequence hybridizes under low stringency conditions to a polynucleotide sequence selected from:
(a) a polynucleotide sequence selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO:25, or a complementary polynucleotide sequence thereof; (b) a polynucleotide sequence encoding a polypeptide selected from SEQ ID NO:26 to SEQ ID NO:50, or a complementary polynucleotide sequence thereof; and (c) a polynucleotide sequence comprising a fragment of (a) or (b), wherein the fragment encodes a polypeptide having at least one fumonisin detoxification activity or at least one fumonisin derivative detoxification activity.
- 44. The nucleic acid of claim 43, wherein said polynucleotide sequence hybridizes under medium stringency conditions to a polynucleotide selected from:
(a) a polynucleotide sequence selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO:25, or a complementary polynucleotide sequence thereof, (b) a polynucleotide sequence encoding a polypeptide selected from SEQ ID NO:26 to SEQ ID NO:50, or a complementary polynucleotide sequence thereof; and (c) a polynucleotide sequence comprising a fragment of (a) or (b), wherein the fragment encodes a polypeptide having at least one fumonisin detoxification activity or at least one fumonisin derivative detoxification activity.
- 45. An isolated or recombinant nucleic acid comprising a polynucleotide sequence selected from:
(a) a polynucleotide sequence selected from the group consisting of SEQ ID NO:1 to SEQ ID NO:25, or a complementary polynucleotide sequence thereof; (b) a polynucleotide sequence encoding a polypeptide selected from SEQ ID NO:26 to SEQ ID NO:50, or a complementary polynucleotide sequence thereof; (c) a polynucleotide sequence which hybridizes under highly stringent conditions over substantially the entire length of a polynucleotide sequence encoding a polypeptide selected from sequence (a) or (b), or a complementary polynucleotide sequence thereof; and, (d) a polynucleotide sequence comprising a fragment of (a), (b) or (c), wherein the fragment encodes a polypeptide having at least one fumonisin detoxification activity or at least one fumonisin-derivative detoxification activity.
- 46. The nucleic acid of claim 45, wherein said polynucleotide sequence hybridizes under highly stringent conditions over substantially the entire length of a polynucleotide sequence encoding a polypeptide selected from SEQ ID NO:26 to SEQ ID NO:50, or a complementary polynucleotide sequence thereof.
- 47. The nucleic acid of claim 45, wherein said polynucleotide sequence is selected from:
(a) a polynucleotide sequence which hybridizes under highly stringent conditions over substantially the entire length of a polynucleotide sequence selected from SEQ ID NO: 1 to SEQ ID NO: 25, or a complementary polynucleotide sequence thereof; and, (b) a polynucleotide sequence comprising a fragment of (a), wherein the fragment encodes a polypeptide having at least one fumonisin detoxification activity or at least one fumonisin-derivative detoxification activity.
- 48. The nucleic acid of claim 47, wherein said polynucleotide sequence hybridizes under highly stringent conditions over substantially the entire length of a polynucleotide sequence selected from SEQ ID NO: 1 to SEQ ID NO:25, or a complementary polynucleotide sequence thereof.
- 49. The nucleic acid of claim 33, wherein the polynucleotide encodes a fumonisin amine oxidase.
- 50. The nucleic acid of claim 33, wherein the optimum pH of said fumonisin detoxification activity is lower for said polypeptide than for any of the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 51. The nucleic acid of claim 33, wherein the thermostability of said fumonisin detoxification activity is higher for said polypeptide than for any of the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 52. The nucleic acid of claim 33, wherein said polypeptide has increased fumonisin detoxification activity upon secretion from a eukaryotic cell relative to any of the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 53. The nucleic acid of claim 52, wherein said eukaryotic cell is a plant cell.
- 54. The nucleic acid of claim 52, wherein said eukaryotic cell is a fungal cell.
- 55. The nucleic acid of claim 33, wherein said polypeptide comprises a leader sequence that directs the secretion of the polypeptide from a plant cell.
- 56. The nucleic acid of claim 55, wherein said polypeptide leader sequence is an apoplast targeting sequence.
- 57. The nucleic acid of claim 55, wherein said polypeptide leader sequence is a peroxisomal targeting sequence.
- 58. The nucleic acid of claim 33, wherein the fumonisin is selected from the group consisting of: a fumonisin B1, a fumonisin B2, a fumonisin B3, a fumonisin B4, and a fumonisin C1.
- 59. The nucleic acid of claim 33, wherein at pH 5.5, the kcat of the fumonisin detoxification reaction catalyzed by the polypeptide is higher than the kcat of the fumonisin detoxification reaction catalyzed by any of the polypeptides corresponding ESP002C2, ESP002C3, ESP003C12, RAT011C, RAT011C2, RAT011C4, or any wild-type APAO.
- 60. The nucleic acid of claim 33, wherein at pH 5.5, the fumonisin KM for the fumonisin detoxification reaction catalyzed by the polypeptide is lower than the fumonisin KM for the fumonisin detoxification reaction catalyzed by the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 61. The nucleic acid of claim 33, wherein at pH 5.5, the fumonisin kcat/KM of the fumonisin detoxification reaction catalyzed by the polypeptide is higher than the fumonisin kcat/KM of the fumonisin detoxification reaction catalyzed by any of the polypeptides corresponding to ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 62. The nucleic acid of claim 33, comprising a promoter operably linked to the polynucleotide.
- 63. The nucleic acid of claim 62, wherein the promoter is tissue-specific.
- 64. A non-native variant of the nucleic acid of claim 33, wherein one or more amino acids of the encoded polypeptide have been mutated.
- 65. A nucleic acid construct comprising a promoter operably linked to the polynucleotide of claim 33.
- 66. The nucleic acid construct of claim 65, wherein the promoter is heterologous with respect to the polynucleotide and effective to cause sufficient expression of the encoded polypeptide to cause the detoxification of fumonisin.
- 67. The nucleic acid construct of claim 66, wherein the polynucleotide sequence of claim 33 functions as a selectable marker.
- 68. The nucleic acid construct of claim 66, wherein a parental codon of the polynucleotide sequence of claim 33 has been replaced by a synonymous codon that is preferentially used in a plant relative to the parental codon.
- 69. The nucleic acid construct of claim 65, wherein the construct is a vector.
- 70. The vector of claim 69 wherein the vector comprises a first polynucleotide sequence comprising the promoter operably linked to the polynucleotide of claim 33 and a second polynucleotide sequence encoding a second polypeptide that confers a detectable phenotypic trait upon a cell or organism expressing the second polypeptide at an effective level.
- 71. The vector of claim 70, wherein the detectable phenotypic traits consists of herbicide resistance, pest resistance, or a visible marker.
- 72. The vector of claim 69, wherein the vector comprises a T-DNA sequence.
- 73. The vector of claim 69, wherein the vector is a plant transformation vector.
- 74. A cell comprising at least one nucleic acid of claim 33, wherein the nucleic acid is heterologous to the cell.
- 75. The cell of claim 74, wherein the polynucleotide of claim 33, is operably linked to a regulatory sequence.
- 76. A cell transduced by the vector of claim 70.
- 77. The cell of claim 74, wherein the cell is a transgenic plant cell.
- 78. The transgenic plant cell of claim 77, wherein the plant cell expresses an exogenous polypeptide with fumonisin detoxification activity.
- 79. The cell of claim 78, wherein the fumonisin is a class B fumonisin.
- 80. The cell of claim 78, wherein the fumonisin is FB1.
- 81. A transgenic organism comprising the nucleic acid of claim 33 or the cell of claim 74.
- 82. The transgenic organism of claim 81, wherein the organism is a plant.
- 83. The transgenic plant of claim 82, wherein the plant is selected from the genera: Fragaria, Lotus, Medicago, Onobrychis, Trifolium, Trigonella, Vigna, Citrus, Linum, Geranium, Manihot, Daucus, Arabidopsis, Brassica, Raphanus, Sinapis, Atropa, Capsicum, Datura, Hyoscyamus, Lycopersicon, Nicotiana, Solanum, Petunia, Digitalis, Majorana, Cichorium, Helianthus, Lactuca, Bromus, Asparagus, Antirrhinum, Heterocallis, Nemesia, Pelargonium, Panicum, Pennisetum, Ranunculus, Senecio, Salpiglossis, Cucumis, Browaalia, Lolium, Malus, Apium, Gossypium, Vicia, Lathyrus, Lupinus, Pachyrhizus, Wisteria, and Stizolobium.
- 84. The transgenic plant of claim 82, wherein the plant is a crop plant selected from the genera: Agrostis, Phleum, Dactylis, Sorgum, Setaria, Zea, Oryza, Triticum, Secale, Avena, Hordeum, Saccharum, Poa, Festuca, Stenotaphrum, Cynodon, Coix, Olyreae, Phareae, Glycine, Pisum, Cicer, Phaseolus, Lens, and Arachis.
- 85. The transgenic plant of claim 82, wherein the plant is selected from: corn, rice, cotton, soybean, sorghum, wheat, oat, barley, millet, sunflower, rapeseed, canola, pea, bean, lentil, peanut, yam, bean, cowpea, velvet bean, clover, alfalfa, lupine, vetch, lotus, sweet clover, wisteria, sweetpea, and a nut plant.
- 86. The transgenic plant of claim 85, wherein the plant is corn.
- 87. A seed produced by the transgenic plant of claim 85.
- 88. The transgenic organism of claim 81, wherein the organism is a microorganism.
- 89. A composition comprising at least two different nucleic acids of claim 33.
- 90. The composition of claim 89 comprising at least ten different nucleic acids of claim 33.
- 91. A composition produced by cleaving one or more nucleic acids of claim 33.
- 92. A method for producing a variant of a nucleic acid of claim 33 comprising recursively recombining a polynucleotide of claim 33 with a second polynucleotide, thereby forming a library of variant polynucleotides.
- 93. The method of claim 92, comprising selecting a variant polynucleotide from the library on the basis of fumonisin detoxification activity.
- 94. The method of claim 93, wherein the recursive recombination is performed in vitro.
- 95. A library of variant polynucleotides produced by the method of claim 93.
- 96. A population of cells comprising the library of claim 95.
- 97. A recombinant polynucleotide produced by the method of claim 93.
- 98. A nucleic acid which comprises a unique subsequence in a nucleic acid selected from SEQ ID NO: 1 to SEQ ID NO:25, wherein the unique subsequence is unique as compared to a nucleic acid corresponding to any of: ESP002C2, ESP002C3, ESP003C12, RAT011C1, RAT011C2, RAT011C4, or any wild-type APAO.
- 99. A method of detoxifying, degrading, neutralizing, or modifying at least one mycotoxin or mycotoxin-derivative, comprising incubating the at least one mycotoxin or mycotoxin-derivative and at least one polypeptide of claim 2, wherein the at least one polypeptide detoxifies, degrades, neutralizes or modifies the at least one mycotoxin or mycotoxin-derivative.
- 100. The method of claim 99, wherein said mycotoxin is a fumonisin, a fumonisin derivative or a fumonisin analog.
- 101. The method of claim 100, wherein the fumonisin, fumonisin derivative or fumonisin analog is present in harvested grain.
- 102. The method of claim 100, wherein detoxification, degradation, neutralization or modification occurs during the processing of harvested grain.
- 103. A method of producing a polypeptide, the method comprising:
(a) introducing into a population of cells a nucleic acid of claim 33, the nucleic acid operatively linked to a regulatory sequence effective to produce the encoded polypeptide; (b) culturing the cells in a culture medium to produce the polypeptide; and, (c) isolating the polypeptide from the cells or from the culture medium.
- 104. A method of producing a transgenic plant or plant cell comprising:
(a) transforming a plant or plant cell with a polynucleotide of claim 33; and (b) optionally regenerating a transgenic plant from the transformed plant cell.
- 105. A method for selecting a plant or cell containing a nucleic acid construct, the method comprising:
(a) providing a transgenic plant or cell containing a nucleic acid construct, wherein the nucleic acid construct comprises a nucleic acid of claim 33; and (b) growing the plant or cell in the presence of a fumonisin under conditions where a polypeptide is expressed at an effective level, whereby the transgenic plant or cell grows at a rate that is discernibly greater than the plant or cell would grow if it did not contain the nucleic acid construct.
- 106. A method of reducing pathogenicity of a fungus producing fumonisin comprising:
(a) providing a transgenic cell containing the nucleic acid of claim 33 operably linked to a promoter, wherein the nucleic acid is heterologous to the cell; and (b) expressing the nucleic acid at a level effective to detoxify fumonisin, thereby reducing the pathogenicity of the fungus.
- 107. The method of claim 106, wherein the cell is a plant cell residing in a plant.
- 108. The method of claim 106, wherein the cell is a microorganism.
- 109. The method of claim 106, wherein the cell comprises a fumonisin esterase encoding polynucleotide operably linked to a promoter.
- 110. A method of detecting fumonisins comprising:
(a) introducing the polypeptide of claim 2, into a sample containing fumonisin; (b) allowing the polypeptide to catalyze the deamination of fumonisin; and, (c) detecting a product of the deamination reaction.
- 111. The method of claim 110, wherein the product of the deamination reaction that is detected is ammonia or hydrogen peroxide.
- 112. A transgenic plant or transgenic plant explant that expresses the polypeptide of claim 2.
- 113. The transgenic plant or transgenic plant explant of claim 112 that further expresses a polypeptide selected from the following: a polypeptide having fumonisin modification activity, a polypeptide having chitinase activity, a polypeptide having antifungal activity, a polypeptide having mycotoxin detoxification activity, a polypeptide having herbicidal activity, a polypeptide having pesticidal activity, and a polypeptide having nematicidal activity.
- 114. The transgenic plant or transgenic plant explant of claim 113 that expresses fumonisin esterase activity.
- 115. The transgenic plant or transgenic plant explant of claim 113, wherein the further expressed polypeptide functions as a selectable marker.
- 116. The transgenic plant or transgenic plant explant of claim 114, wherein the selectable marker consists of one or more of: herbicide resistance, pest resistance, or a visible marker.
- 117. A method of reducing pathogenicity of a fungus producing fumonisin comprising:
a) providing a transgenic cell containing the nucleic acid of claim 33, operably linked to a promoter, wherein the nucleic acid is heterologous to the cell; and, b) expressing the nucleic acid at a level effective to produce sufficient H2O2 to reduce fungal infection.
- 118. The method of claim 117, wherein the cell is a plant cell residing in a plant.
- 119. The method of claim 117, wherein the cell comprises a fumonisin esterase encoding polynucleotide operably linked to a promoter.
- 120. A method of producing a transgenic organism, the method comprising:
a) introducing into an organism, a nucleic acid of claim 33; and, b) expressing a polypeptide encoded by the nucleic acid at a level effective to deaminate fumonisin.
- 121. The method of claim 120, wherein the organism is selected from the group consisting of: a plant, a fungus, and a bacteria.
CROSS-REFERENCES TO RELATED APPLICATIONS
[0001] Pursuant to 35 USC §119(e), this application claims priority to and benefit of U.S. Provisional Patent Application Serial Nos. 60/266,918, filed on Feb. 6, 2001, and 60/300,324, filed on Jun. 22, 2001, the disclosures of each of which are incorporated herein in their entirety for all purposes.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60266918 |
Feb 2001 |
US |
|
60300324 |
Jun 2001 |
US |