Claims
- 1. A polynucleotide having the sequence of SEQ ID NO: 1 and conservatively modified variants thereof.
- 2. The polynucleotide of claim 1 wherein said polynucleotide is DNA.
- 3. A polynucleotide having the sequence of SEQ ID NO: 2 and conservatively modified variants thereof.
- 4. The polynucleotide of claim 3 wherein said polynucleotide is DNA.
- 5. A polynucleotide having the sequence of SEQ ID NO: 3 and conservatively modified variants thereof.
- 6. The polynucleotide of claim 5 wherein said polynucleotide is DNA.
- 7. A glucose oxidase protein having the amino acid sequence of SEQ ID NO: 4 and conservatively modified variants thereof.
- 8. A glucose oxidase protein having the amino acid sequence of SEQ ID NO: 5 and conservatively modified variants thereof.
- 9. A glucose oxidase protein having the amino acid sequence of SEQ ID NO: 6 and conservatively modified variants thereof.
- 10. A recombinant polynucleotide comprising a vector and a gene wherein said gene encodes an insect glucose oxidase protein.
- 11. The polynucleotide vector of claim 9 wherein said gene is selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 3.
- 12. The recombinant polynucleotide of claim 9 wherein said vector is selected from the group cosisting of pGEM-T Easy, pBK-CMV, pET28a(+), pCAMBIA, pBI101, pGA482 and pRT-100.
- 13. A method for producing large quantities of the polypeptide of claim 5 comprising:
introducing the recombinant polynucleotide of claim 9 into host cells so as to yield transformed host cells; inducing expression the protein encoded by said polynucleotide sequence; and, extracting said polypeptide from said host cells.
- 14. A method for creating a plant having enhanced resistance to insect predation comprising:
introducing the recombinant polynucleotide of claim 9 into the cells of a plant so as to yield transformed plant cells; and, regenerating said transformed plant cells to provide a differentiated plant wherein said plant expresses the polypeptide encoded by said gene.
- 15. The transgenic plant formed by the method of claim 14.
- 16. The transgenic plant of claim 15 wherein said plant is a commercially grown crop.
- 17. The transgenic plant of claim 16 wherein said plant is tobacco.
- 18. A seed of the transgenic plant formed by the method of claim 14.
- 19. An antibody that binds specifically to an insect glucose oxidase protein.
- 20. The antibody of claim 19 wherein said glucose oxidase protein is selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 5, and SEQ ID NO: 6.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part of U.S. patent application Ser. No. 09/205,630, filed Dec. 3, 1998, the teachings of which are expressly incorporated herein by reference, which is a continuation-in-part of U.S. provisional patent application Ser. No. 60/067,457, filed Dec. 4, 1997, the teachings of which are expressly incorporated herein by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60067457 |
Dec 1997 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09205630 |
Dec 1998 |
US |
Child |
09956326 |
Sep 2001 |
US |