Claims
- 1. A method comprising:
providing first and second subtrates and a seal; placing the second substrate on top of the seal and first substrate so as to define a closed chamber therebetween, said chamber adapted to retain a quantity of fluid so that the fluid is in contact with both inner surfaces, and wherein at least one of said inner surfaces is functionalized with polynucleotides, polypeptides, or polysaccharides; and introducing a fluid containing a plurality of components into the closed chamber so as to provide a quantity of fluid therein in contact with both inner surfaces.
- 2. A method according to claim 1 wherein the polynucleotide is a polyribonucleotide.
- 3. A method according to claim 1 wherein the chamber is adapted to retain a film of fluid in contact with both inner surfaces.
- 4. A method according to claim 3 wherein the chamber is up to several millimeters in thickness.
- 5. A method according to claim 4 wherein the inner surfaces of the first and second substrates are substantially parallel.
- 6. A method according to claim 4 wherein the chamber is up to three millimeters in thickness.
- 7. A method according to claim 6 wherein the chamber is up to two millimeters in thickness.
- 8. A method according to claim 1 wherein the fluid is introduced into the closed chamber by first introducing the fluid onto the first substrate carrying the seal then placing the second substrate on top of the seal.
- 9. A method according to claim 1 wherein the seal is attached to the first substrate.
- 10. A method according to claim 1 wherein the inner surfaces of the first substrate is functionalized with the polynucleotides, polypeptides, or the polysaccharides.
- 11. A method according to claim 1 wherein the inner surface of the second substrate is functionalized with the polynucleotides, polypeptides, or the polysaccharides.
- 12. A method according to claim 1 wherein the inner surface of the first and second substrate is each functionalized with polynucleotides, polypeptides, or the polysaccharides.
- 13. A method according to claim 1 wherein one of the substrates has an opening for introducing fluid into the closed chamber.
- 14. A method according to claim 1 wherein the seal has an opening for introducing fluid into the closed chamber.
- 15. The method of claim 1 wherein the at least one of the inner surfaces is functionalized with polynucleotides.
- 16. The method of 1 wherein the at least one of the inner surfaces is functionalized with polypeptides.
- 17. An apparatus comprising
first and second subtrates and a seal, wherein the second substrate is positioned on top of the seal and first substrate so as to define a closed chamber therebetween, said chamber retaining a quantity of fluid therein so that the fluid is in contact with both inner surfaces, and wherein at least one of said inner surfaces is functionalized with polynucleotides, polypeptides, or polysaccharides.
- 18. An apparatus method according to claim 17 wherein the chamber is up to several millimeters in thickness.
- 19. An apparatus according to claim 18 wherein the inner surfaces of the first and second substrates are substantially parallel.
- 20. An apparatus according to claim 18 wherein the chamber is up to three millimeters in thickness.
- 21. An apparatus according to claim 17 wherein the inner surface of the first and second substrate is each functionalized with polynucleotides, polypeptides, or the polysaccharides.
- 22. An apparatus according to claim 17 wherein one of the substrates has an opening for introducing fluid into the closed chamber.
- 23. An apparatus according to claim 17 wherein the seal has an opening for introducing fluid into the closed chamber.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation of U.S. patent application Ser. No. 09/782,542 (U.S. Pat. No. 6,513,968) which is a continuation of U.S. patent application Ser. No. 09/137,963 (U.S. Pat. No. 6,186,659). Priority is claimed from both of the foregoing applications under 35 U.S.C. 120 and those applications are incorporated herein by reference.
Continuations (2)
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Number |
Date |
Country |
Parent |
09782542 |
Feb 2001 |
US |
Child |
10358783 |
Feb 2003 |
US |
Parent |
09137963 |
Aug 1998 |
US |
Child |
09782542 |
Feb 2001 |
US |