Claims
- 1. A cuvette for simultaneously monitoring and recording an individual light emission in an array of reaction chambers comprising:
(a) a detection chamber, comprising an opening adapted to accept an array that has a planar surface with at least 400,000 reaction chambers disposed thereon, wherein the reaction chambers have a center to center spacing of between 5 to 200 μm and each chamber has a width in at least one dimension of between 0.3 μm and 100 μm, said detection chamber further comprising at least one surface or opening that is transparent to said light emission, said reaction chamber adapted to receive at least one analyte; (b) a receptacle within said detection chamber to align said array with an optical detector; and (c) affluent and effluent outlets connected to said detection chamber to allow said array to be in fluid communication with one or more reagent reservoirs.
- 2. The cuvette of claim 1 further comprising a plurality of optical fibers that forms an optical fibers bundle; wherein said bundle is capable of transmitting light emitted from said reaction chamber to said optical detection means; and wherein each optical fiber is aligned to transmitted the light emission of only one said reaction chamber.
- 3. The cuvette of claim 1 wherein the optical fiber bundle contains at least one optical fiber per reaction chamber.
- 4. The cuvette of claim 1 wherein the optical fiber bundle contains at least 4 optical fibers per reaction chamber.
- 5. The cuvette of claim 1 wherein said detection chamber is adapted to receive a fluid optical coupler to transmit said light emission to said optical detector.
- 6. The cuvette of claim 5 wherein said fluid optical coupler is microscope oil.
- 7. The cuvette of claim 5 wherein said fluid optical coupler is an aqueous solution.
- 8. The cuvette of claim 1 wherein said array is in optical communication with said optical detector through an optical lens.
- 9. The cuvette of claim 8 wherein said optical lens is a compound lens containing more than one optical element.
- 10. The cuvette of claim 1 further comprising an optical detection system adapted to detect light emission from each said reaction chamber.
- 11. The cuvette of claim 1 wherein said optical detection system is a CCD camera.
- 12. The cuvette of claim 11 wherein said CCD camera comprise at least one individually addressable pixel per said reaction chamber.
- 13. The cuvette of claim 11 wherein said CCD camera comprise at least 4 individually addressable pixel per said reaction chamber.
- 14. The cuvette of claim 1 further comprising thermo coupling means to connect said array to a thermal source.
- 15. The cuvette of claim 10 wherein said thermal source is selected from the group consisting of a heating source, a cooling source, or a combination thereof.
- 16. The cuvette of claim 1 further comprising a reagent delivery means between said affluent outlet and said one or more reagent reservoir.
- 17. The cuvette of claim 12 wherein said reagent delivery means is capable of delivering an activated nucleotide 5′ triphosphate precursor of one known nitrogenous base to each said reaction chamber.
- 18. The cuvette of claim 13 wherein said activated nucleotide 5′ triphosphate is selected from the group consisting of ATP, GTP, TTP, CTP and UTP.
- 19. The cuvette of claim 12 wherein said reagent delivery means is connected to one or more reagent reservoirs to sequentially add two or more activated nucleotide 5′ triphosphate precursor of one known nitrogenous base to each said reaction chamber.
- 20. The cuvette of claim 1 further containing reversible coupling means at said affluent and effluent outlets to reversibly connect said cuvette to said one or more reagent reservoirs.
- 21. The cuvette of claim 1 further comprising a data collection system in communication with the optical detection system.
- 22. The apparatus of claim 1, wherein the optical detector is a CCD camera.
- 23. The apparatus of claim 1, wherein the analyte is nucleic acid.
- 24. The apparatus of claim 1, wherein the analyte is immobilized on one or more mobile solid supports that are disposed in the reaction chamber.
RELATED APPLICATIONS
[0001] This application claims the benefit of priority to U.S. Ser. No. 10/104,280 filed Mar. 21, 2002; which is a CIP of Ser. No. 09/814,338 filed Mar. 21, 2001; which is a CIP of U.S. Ser. No. 09/664,197 filed Sep. 18, 2000; which is a CIP of U.S. Ser. No. 09/398,833 filed Sep. 16, 1999, now U.S. Pat. No. 6,274,320. Each of the above referenced patent and patent applications are incorporated herein by reference in their entireties.
Continuations (1)
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Number |
Date |
Country |
Parent |
10104280 |
Mar 2002 |
US |
Child |
10222592 |
Aug 2002 |
US |
Continuation in Parts (3)
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Number |
Date |
Country |
Parent |
09814338 |
Mar 2001 |
US |
Child |
10104280 |
Mar 2002 |
US |
Parent |
09664197 |
Sep 2000 |
US |
Child |
09814338 |
Mar 2001 |
US |
Parent |
09398833 |
Sep 1999 |
US |
Child |
09664197 |
Sep 2000 |
US |