Claims
- 1. A microfluidic system for determining relative positions in a target nucleic acid sequence that are occupied by a given nucleotide, comprising:
a microscale separation channel having first and second ends for separating nucleic acid fragments by size; a first source of a first nested set of fragments of the target nucleic acid sequence in fluid communication with the separation channel, each of the fragments in the first nested set terminating at a different position occupied by the given nucleotide; a second source of a second nested set of fragments of the target nucleic acid sequence in fluid communication with the separation channel, each of the fragments in the second nested set terminating in a nucleotide different from the given nucleotide; a means for mixing a first concentration of the first nested set with a second concentration of the second nested set in a first mixture, the first concentration being determinably different from the second concentration; a means for injecting a portion of the first mixture into the separation channel and transporting the portion through the separation channel; and a means for detecting separate fragments of the first and second nested sets in the separation channel.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This application is a continuation of U.S. patent application Ser. No. 09/598,807, filed Jun. 21, 2000, which is a continuation of U.S. patent application Ser. No. 09/334,527, filed Jun. 16, 1999, now U.S. Pat. No. 6,107,044, which is a continuation of U.S. patent application Ser. No. 08/953,005, filed Oct. 16, 1997, now U.S. Pat. No. 5,958,694, the full disclosures of which are incorporated herein by reference in its entirety.
Continuations (3)
|
Number |
Date |
Country |
Parent |
09598807 |
Jun 2000 |
US |
Child |
09952049 |
Sep 2001 |
US |
Parent |
09334527 |
Jun 1999 |
US |
Child |
09598807 |
Jun 2000 |
US |
Parent |
08953005 |
Oct 1997 |
US |
Child |
09334527 |
Jun 1999 |
US |