Patent Application
20230165995
The present invention relates to an apparatus for the sterilization of ambient air by means of a laser beam filter.
In more detail, the present invention relates to an apparatus for the sterilization of air from any bacterium, virus, dust or any other organic or non-organic body present in the air, comprising dangerous insects, of a public or private space, using a sterilization chamber provided with a specific laser beam filter.
Aerosol in air is one of the fastest means of contagion between people for pathogens such as Covid-19 or other viruses and bacteria.
Contagion mainly occurs when infected people sneeze, cough or speak in areas where people gather or are closely exposed to one another.
The internal circulation of air in closed spaces facilitates the diffusion of these microorganisms in droplets, because they can float in the air and travel at a certain distance due to air recirculation.
In addition to viruses and bacteria which contaminate in an airborne manner, in some regions of the world there are other risk factors associated with small flying insects, in some cases capable of transferring serious pathogens such as malaria.
The current air sterilization devices consist of several systems which use:
Other systems simultaneously use one or more of the systems just described. These lamps have the problem that they are very polluting for the space during the disposal step and must be treated as special waste.
Furthermore, systems exist which use a collimated or scanning laser beam by means of complex, moving optical systems. These systems are very delicate and expensive, use dynamic motion systems and poorly stable and expensive laser sources which require frequent, complex, and expensive periodic maintenance.
Other systems convey air through cones or channels so that the air passes through a fixed laser beam. The interior of these systems remains polluted by viruses and bacteria, contaminating the space during maintenance or inspection.
All these systems use either CW lasers or YAG lasers with fast pulses and moving through scanners, but in these cases the irradiance and wavelengths involved do not ensure the air sterilization.
In other systems, wavelengths in the UVA, UVB and UVC range are used, but the very long exposure times (generally greater than 1200 seconds, but also beyond three hours for a more reliable result) and the danger of these ionizing wavelengths for people do not allow their use in the presence thereof, and moreover these wavelengths deteriorate the components made of plastic material, such as gaskets.
Finally, these radiant systems have the limit of dark areas when the spaces are scanned, areas caused by the presence of many objects occupying these spaces, in addition to the fact that in the case of insects, the killing must use electrical systems and the residues must be removed manually. After the life cycle of these lamps, the disposal thereof is polluting for the space and they must be disposed of as special waste.
Even ozone cannot be used in populated spaces, and the same applies to combined ozone-UV lamp systems.
But even if these systems were used in the absence of people, the most dangerous moment for contagion occurs when the spaces are repopulated, because only a minimal probability of contagion exists through contact with surfaces.
Almost all of these systems are not transportable, are bulky or in particular require fixed installations. Furthermore, they cannot be used for other purposes and therefore the installation space cannot be recovered.
Other systems use photocatalysis technology. Titanium dioxide (TiO2) produces a strong oxidative effect in the presence of ultraviolet (UV) light or visible light and therefore can be used as a photocatalytic disinfectant. Although many studies have been reported on the photocatalytic bacteria inactivation, few studies have addressed virus inactivation. This technology is historically used to combat air pollution deriving mainly from the transport field, the industrial field, the activity of power plants and incinerators, from domestic heating, from the use of pesticides in the agricultural field and from dust deriving from the mining field.
The problem with these systems is the disposal of the filter consisting of titanium dioxide and a UV lamp, for example a mercury vapor lamp which emits in the UVC band.
This type of filter must be replaced and disposed of as special waste.
U.S. Pat. No. 4,115,280A describes an apparatus with a chamber for deactivating or activating the biological or chemical activity of macromolecular species, which uses laser radiation at a frequency which excites the vibrational and rotational states of the irradiated species (including viruses and bacteria) and at a sufficient intensity to activate the species (but below the denaturation level) or to a level such that the weak bonds—for example the hydrogen bonds—which determine the spatial character, and therefore the biological activity of the macromolecules, are irreversibly broken to such an extent that the macromolecule loses its original form (the denaturation process) and takes an inactive (denatured) configuration. One of the problems of this apparatus is that the species to be inactivated are substantially on a movable surface and the incidence of the laser beam is not efficient, as the laser is expanded and/or focused and angled as a function of the beam features thereof as well as of the chamber size, and even in this case the actual efficacy on viruses and bacteria is not demonstrated. The apparatus is designed in a complex manner, needs beam absorbers to treat the gases, is expensive and not transportable.
US 2013/248734 describes an air purification apparatus. Energy beams are used which form one or more energy fields inside a chamber to produce an outflow of sterilized air. In order not to deposit contamination residues on the surfaces of the ducts through which the air to be sterilized passes, a load generation system is implemented to repel the particles from the chamber walls, which simply adds to the general principle described by U.S. Pat. No. 4,115,280A and therefore it does not solve the problems already indicated.
JP 2000126549 relates to a system for the decomposition of an exhaust gas from an incinerator or the like, through irradiation with a CO2 laser beam. Since a large number of CO2 molecules is included in the exhaust gas, the CO2 molecules are instantly heated by the CO2 laser beam. A dioxin molecule is adsorbed by the incineration of ash or the like, and when the CO2 molecule is heated, the CO2 molecule collides with the dioxin molecule and the thermal energy owned by the CO2 molecule is transferred to the dioxin molecule so that the dioxin molecule is actually heated and decomposed. No application to viruses or bacteria is described, and beam absorbers are used, leaving all the above problems intact.
There is a need for a laser sterilizer with proven efficacy on viruses and bacteria in gases, which is economical, transportable and possibly does not require beam absorbers and optionally does not require movable surfaces.
It is the aim of the present invention to provide a device which solves the problems and overcomes the drawbacks of the prior art.
It is subject-matter of the present invention a device according to the appended claims, which form an integral part of the present description.
The invention will now be described by way of example, with particular reference to the drawings of the accompanying figures, in which:
It is worth noting that hereinafter elements of different embodiments may be combined together to provide further embodiments without restrictions respecting the technical concept of the invention, as a person skilled in the art will effortlessly understand from the description.
The present description also refers to the prior art for its implementation, with regard to the detail features which not described, such as, for example, elements of minor importance usually used in the prior art in solutions of the same type.
When an element is introduced it is always understood that there may be “at least one” or “one or more”.
When a list of elements or features is given in this description it is understood that the invention according to the invention “comprises” or alternatively “consists of” such elements.
According to an aspect of the present invention, an apparatus is provided which incorporates a sterilization chamber in which the ambient air is flowed at a sufficiently low speed by means of a ventilation system, while the chamber is sufficiently filled with a laser beam. Preferably, the air speed can be between 0.01 and 10 m/s (depending on the wavelength used for the laser, the number of laser segments, and the power or irradiance, it is preferably below 3 m/s), so that a given volume of air remains under the action of the laser beam for at least 5 ms, preferably for about 30 ms (in general, the air speed will depend on the number of laser segments between two mirrors, as well as on the wavelength and a predetermined irradiance of the laser beam, as defined below).
Although the present invention always refers to air to be sterilized, the suggested device is also effective for the sterilization of any other non-flammable gas.
Referring to
The portions 102 adjacent to the end portions 101 contain the ventilation and/or aspiration system, by means of electronically controlled fans. The lower part 102 (closest to the wheels in the example) may also include a system of ultrasonic proximity sensors and an RGB LED light indicator which indicates the operating states of the invention. The proximity sensors allow to detect the presence or passage of people. Based on this detection, the ventilation system regulates the airflow and the laser power, moving from a low power consumption condition, lowering the laser power and the aspiration flow rate when it does not detect people or the movement of people in the vicinity of the device, to the maximum power of the intake airflow and of the laser power when the sensors detect the presence or passage of people in the vicinity of the device 100. The section indicated by 104 diagrammatically shows the laser optics, the beam expander, which expands the beam (up to a size comparable with or equal to the size—width—of the mirrors 112,113) and reduces the angle thereof to a size for example ≤0.7 mrad (generally less than 1 mrad, preferably between 0.3 and 2 mrad), creating the parallelism which allows the beam to go through tens of meters bouncing between the two mirrors and saturating the laser beam filter chamber. This saturation allows to lower the power of the laser used and thus to reduce the costs of the apparatus.
Section 105 diagrammatically shows the laser beam generator and the power supply thereof.
Other values and provisions are possible respecting the concept of saturation of the sterilization chamber by the laser beam.
According to an aspect of the invention, the sterilization chamber 110 may have a length (height in the figure) between 100 and 1000 cm. According to another aspect of the invention, the sterilization chamber may have a width between 20 and 1000 cm (perpendicular to the vertical in the plane of the sheet). According to a further aspect of the invention, the sterilization chamber may be 2 to 8 cm thick (perpendicular to the sheet, thickness not shown).
Referring also to
The means 116 are particularly effective in avoiding the deposit of material on the mirrors and on the walls of the chamber. These are optional means, and more effective and unexpensive than the loads of patent application US2013/248734.
Referring also to
According to an aspect of the invention, after the inlet inclination of the laser, it is also possible to reflect it alternately with a path perpendicular to the advancement direction and again in an inclined manner (with an angle between 0.2 and 1° or between 1 and 15°, not necessarily identical to the inlet angle), so as to thicken the laser network and thus increase the air treatment time (or decrease the laser power). This can be done on the forward path and/or on the return path.
The sampling described is advantageous in that it maximizes the meeting between the air to be purified and the laser beam.
Advantageously, the mirrors can be placed on two opposite sides of the parallelepiped sterilization chamber. In this case, the laser will be positioned so that the laser beam first strikes one of such mirrors with a non-zero angle, at a first end of a smaller side, and is then reflected several times from one smaller side to the other. According to an embodiment of the invention, a mirror with a certain angle is placed at a second end of a smaller side in order to force the laser beam to the opposite path, but with a different inclination angle, so that the entire laser path covers the entire volume.
According to an aspect of the invention, the mentioned laser source may have an average power from 1 to 1000 W CW as a function of the volume of the sterilization chamber, or pulse of any modulation frequency range and duty cycle, but always with an average power from 1 to 1000 W. The wavelength will be specified later in this description. The temperature of the laser source may be kept constant by means of a Peltier or air-cooling system, from the same airflow to be sterilized and/or with a heatsink which may be of aluminum or with a heat pipe. A driver may supply the laser source with constant current or voltage and a photodiode or thermopile system may control the power thereof, preferably constant. According to an aspect of the invention, a thermal sensor monitors and maintains the constant temperature of the laser source, modulating the current to the cooling system.
The laser source may be formed by a single laser beam or by multiple beams which cover all or part of the width of the smaller side inside the parallelepiped-shaped sterilization chamber.
With regard to the inclination of the laser beam(s), they can be inclined by an angle α at the source between 0.2° and 1° or between 1° and 15° (applicable to all embodiments) with respect to a direction perpendicular to the two smaller internal sides. Thereby, the laser beam(s) bounce from mirror to mirror multiple times until they reach the top corner inside the sterilization chamber. These values can also be applied to the return mirror 114 (optional, depending on the beam power needed to destroy the particular virus, it is possible to have multiple return mirrors if there are multiple laser beams), however the inclination β of the latter (i.e., of the beam which bounces back) must be different from the inclination of the beam 115 to the source (features applicable to all embodiments). Thereby, a return “laser network” may be formed by means of the subsequent bouncing between the opposite mirrors, which further completes the “laser beam network” which completely fills the sterilization chamber inside the parallelepiped, creating a laser beam filter which sterilizes the air flowing in the chamber.
Referring to
The airflow in the device 100 is better clarified by
Optionally, at the two air inlet and outlet ends of the device according to the invention a simple filter is placed (such as the dustproof type) but of a color and a material which does not absorb the wavelength of the laser used, to prevent large bodies (such as any insects or the like) from entering the sterilization chamber. Alternatively, the small-sized insects may be allowed to enter to be destroyed by the laser, as will be seen later in the present description. Furthermore, the inlet and outlet filters may alternatively or additionally have a safety function in the event of device failure, preventing the laser beam(s) from exiting the sterilization chamber, or foreign bodies from entering through the sterilized air outlet.
According to an aspect of the invention, one or more safety sensors are placed in various positions (for example to avoid opening the sterilization chamber while the device is operating), for example at the inlet and outlet of the sterilization chamber. Two switch buttons connected in series may also be provided, which immediately interrupt the laser supply, with interlock function, if the sterilization chamber is opened in some manner (for example a panel is removed which forms the parallelepiped containing the sterilization chamber during an inspection and/or periodic cleaning).
When the invention is in the form of a parallelepiped, it may be fixed to a wall in a vertical or horizontal position, to a ceiling in a longitudinal or transverse position, or hung from a ceiling, horizontally or vertically by means of metal cables or other means. The device may be provided with wheels (see
Referring to
Such panels 150 may for example be of any metal or plastic material.
The panels 150 may have a multiplicity of holes 151 of the same or different size for a homogeneous aspiration in the whole surface of the panels and serve as air collectors. In this case, it is convenient that there is an end 152 which connects to the air inlet in the device 100.
The device according to the invention may be conveniently powered in alternating voltage from 100 to 240V AC from 50 to 60 Hz, or in DC from 12 to 48 Vdc by means of special power supply means.
Referring now to
Although in the previous embodiments a device with a parallelepiped-shaped sterilization chamber has been illustrated, any other useful form which allows the creation of a laser beam network is possible. For example, according to the invention it is possible to obtain the device in the form of a cylinder, where the mirrors are inside on the two circular bases, and the laser beams go through the height of the cylinder with inclination angles like in the other embodiments, where however the angle varies in the two directions of the base, or there are multiple laser sources emitting from one of the two bases or from both (the latter feature also being possible in the other embodiments).
Examples of Sterilization with the Device According to the Invention
The sterilization functionality is explained here according to an example mathematical model. Although the calculation has general value, reference will be made to a specific example in which the sterilization chamber is in the form of a parallelepiped.
Therefore, a parallelepiped is considered with dimensions of length H width W and thickness T. For example, these dimensions may be:
H=1.5 m
W=0.6 m
T=0.04 m
The airflow flows along the length H of the parallelepiped at a speed v, going through the section S of the parallelepiped
S=W·T
A certain airflow rate Q to be sterilized by means of laser radiation inside the parallelepiped is considered.
The air speed inside the parallelepiped must therefore not exceed Vmax
V≤V
max
=Q/S
The exposure time t of the air to the laser radiation must be greater than Tmin:
T≥T
min
=H/V
With the example values above, we obtain:
H=1.5 m
W=0.6 m
T=0.04 m
S=W·T=0.6·0.04=0.024 m2
Q=120 m3/h=0.033 m3/s
V≤V
max
=Q÷S=0.0333/0.024=1.39 m/s
t≥t
min
=H/V=1.5/1.39=1.08 s
The result of this first example shows how the air, in this sterilization chamber, is exposed to the laser beam filter radiation for 1.08 s by going through the laser beam filter at a speed of only 1.39 m/s with a filtered airflow of 120 m3/h.
It should be noted here that the laser manages to fill (sample) the entire sterilization chamber due to the particular positioning of the example of the parallelepiped. In fact, being just 4 cm thick, a laser with a beam width of the same radius is sufficient to ensure sterilization.
As for the angles α and β, they can be chosen in this example between 1 and 5° so that the device is particularly optimized in efficacy. This interval is also valid for the following examples where more reflections or more beams have been used. Under the degree of inclination it is possible and particularly effective in all embodiments in which it is convenient to multiply the reflections of the laser beam, for example when the laser beam enters a small central opening in a wall of the device and is reflected both on the right and on the left and the air exits from both sides, this being a different embodiment from those shown in the figures. Therefore, an optimal range between 0.2 and 5 degrees can also be chosen for the angles α and β in each embodiment.
As for the laser power, the following can be had as an example:
Laser source power=3 W
Irradiance (E)=30 KW/m2
Exposure time (t)=1.08 s
Radiant exposure E*t=32,400 J/m2
A parallelepiped is therefore still considered with dimensions of length H width W and thickness T. For example, these dimensions may always be:
H=1.5 m
W=0.6 m
T=0.04 m
The airflow flows along the length H of the parallelepiped at a speed v, going through the section S of the parallelepiped
S=W·T
A certain airflow rate Q to be sterilized by means of laser radiation inside the parallelepiped is considered. The air speed inside the parallelepiped must therefore not exceed Vmax
V≤V=Q/S
The exposure time t of the air to the laser radiation must be greater than Tmin:
T≥T
min
=H/V
With the example values above, we obtain:
H=1.5 m
W=0.6 m
T=0.04 m
S=W·T=0.6·0.04=0.024 m2
Q=240 m3/h=0.066 m3/s
V≤V
max
=Q÷S=0.0666/0.024=2.77 m/s
t≥t
min
=H/V=1.5/2.77=0.54 s
The result of this second example shows how the air, in this sterilization chamber, is exposed to the laser beam filter radiation for only 0.54 s compared to example No. 1, going through the laser beam filter at a speed of only 2.77 m/s with a filtered airflow of an impressive 240 m3/h.
This is possible by doubling the number of laser beams going through the filter chamber, through a return laser beam which extends in a volume parallel to the forward beam, thus being able to double the thickness of the sterilization chamber (or halve the section of the beam). Using the same concept, the parallel volumes may also be more than two.
Furthermore, it is possible to position other return mirrors at the bottom and at the top with respect to the airflow direction, so as to bounce the laser beam several times at different angles to create an even denser network of laser irradiation segments.
As far as laser power is concerned, it may remain the same, for example:
Laser source power always=3 W
Irradiance (E)=30 KW/m2
Exposure time (t)=1.08 s
The radiant exposure will be double as the number of beams inside the filter chamber have doubled, but with a double airflow compared to example No. 1, in fact the radiant exposure will still remain E*t=32,400 J/m2.
A parallelepiped is therefore still considered with dimensions of length H width W and thickness T. For example, these dimensions may be:
H=1.5 m
W=0.6 m
T=0.04 m
The airflow flows along the length H of the parallelepiped at a speed v, going through the section S of the parallelepiped
S=W·T
A certain airflow rate Q to be sterilized by means of laser radiation inside the parallelepiped is considered. The air speed inside the parallelepiped must therefore not exceed Vmax
V≤V=Q/S
The exposure time t of the air to the laser radiation must be greater than Tmin:
T≥T
min
=H/V
With the example values above, we obtain:
H=1.5 m
W=0.6 m
T=0.04 m
S=W·T=0.6·0.04=0.024 m2
Q=240 m3/h=0.066 m3/s
V≤V
max
=Q÷S=0.0666/0.024=2.77 m/s
t≥t
min
=H/V=1.5/2.77=0.54 s
The result of this third example shows how the air, in this sterilization chamber, is exposed to the laser beam filter radiation for only 0.54 s, going through the laser beam filter at a speed of only 2.77 m/s with a double filtered airflow of 240 m3/h compared to example No. 1.
This is possible by doubling the laser source power, as described in the example:
Laser source power=6 W
Irradiance (E)=60 KW/m2
Exposure time (t)=0.54 s
The radiant exposure will always be the same, but with a double airflow with respect to example No. 1, in fact radiant exposure will still remain E*t=32,400 J/m2.
A parallelepiped is therefore still considered with dimensions of length H width W and thickness T. For example, these dimensions may be:
H=1.5 m
W=0.6 m
T=0.04 m
The airflow flows along the length H of the parallelepiped at a speed v, going through the section S of the parallelepiped
S=W·T
A certain airflow rate Q to be sterilized by means of laser radiation inside the parallelepiped is considered. The air speed inside the parallelepiped must therefore not exceed Vmax
V≤V=Q/S
The exposure time t of the air to the laser radiation must be greater than Tmin:
T≥T
min
=H/V
With the example values above, we obtain:
H=1.5 m
W=0.6 m
T=0.04 m
S=W·T=0.6·0.04=0.024 m2
Q=240 m3/h=0.066 m3/s
V≤V
max
=Q÷S=0.0666/0.024=2.77 m/s
t≥t
min
=H/V=1.5/2.77=0.54 s
The result of this fourth example shows how the air, in this sterilization chamber, is exposed to the laser beam filter radiation for only 0.54 s, going through the laser beam filter at a speed of only 2.77 m/s with a double filtered airflow of 240 m3/h compared to example No. 1.
This is possible by using a wavelength which has a much higher water absorption index than other wavelengths and by adjusting the laser source power, as described in the example:
Laser source power=5.6 W
Irradiance (E)=56 KW/m2
Exposure time (t)=0.54 s
Absorption index>5 times that at 1940 nm wavelength
The radiant exposure will be slightly lower, but the absorption index is much higher than the wavelength at 1940 nm, and with a double airflow compared to Example No. 1, the same results will be obtained if not amplified, leaving room for an even higher increase in airflow. In detail, the radiant exposure here is equal to E*t=30,240 J/m2 but with an efficiency of this radiant exposure at 10600 nm>5 times that at 1940 nm.
It is possible to calculate the airflow speed along shapes other than that of the parallelepiped with the same principles, therefore as a function of the number of laser segments, the laser wavelength and irradiance.
The validity and efficacy of the laser in the destruction of viruses and bacteria, as well as of infection-carrying insects, has been independently assessed by the ICGEB (Centro internazionale di ingegneria genetica e biotecnologie) based in Trieste, Italy. ICGEB is an autonomous intergovernmental organization which operates within the United Nations system and manages 46 state-of-the-art laboratories in different parts of the world.
The optimal wavelengths and irradiances capable of killing viruses emerged from this assessment, as follows: 1940 nm (semiconductor laser or Thulium fiber laser), 2950 nm (Erbium Yag Laser), 9300 nm, 10600 (Co2 laser), with a 15% variation around these values which turn out to be laser absorption peaks by the water. Although other values outside these ranges are also possible, the efficiency of the lasers in these cases would be too low (less than 1%) to be used effectively and conveniently.
As for the 9,300 nm wavelength, it is usable but has two disadvantages:
These wavelengths result in the destruction of viruses and bacteria by photomechanical and photothermal effect. The use of a photochemical effect would require excessively long radiation times, which would not allow to obtain a functional result of this laser beam filter, even at very high irradiances over 150 W/cm2.
Below are some demonstration tests for the wavelengths between blue and infrared. Other similar tests have been carried out up to wavelengths above 12,000 nm, of which only one is reported at 10,600 nm. Although the viruses used are HEK 293T and AAV lentiviral vector, other viruses such as coronavirus have similar structures and dimensions and therefore the device according to the present invention will be effective because it acts on the destruction by local temperature rise (photothermal and photomechanical effects) and not on some photochemical effect.
Test No. 1—Test with 445 nm Blue Laser
As can be seen from
The test was carried out with the same HEK293T cell line but with different cell amounts and irradiations for a time from 1 to 4 seconds:
The blue laser was used with powers 8 J; 24 J; 32 J corresponding to 1, 3, 4 pulses, respectively.
Referring to
Test No. 3—Test with IR Laser
The wavelength used was 1940 nm, with an H2O absorption factor corresponding to 99.3%.
The laser power was 2.4 W, used with two irradiance values of 0.35 W/cm2 and 3 W/cm2 for two seconds of exposure.
The viruses used were:
Referring to
The three wavelengths referred to in the previous tests were tested, but with greater irradiances and exposure time of 1 and 2 seconds (in focus), or 1+1 and 2+2 seconds (in and out of focus: in the first exposure time of 1 and 2 seconds, the laser is focused on the virus drop (in focus), and subsequently a second pulse of 1 and 2 seconds radiates the entire surface of the Eppendorf tubes (out of focus) to avoid the viruses remaining attached to the walls). The wavelengths and irradiances are as follows:
wavelength 445 nm—irradiance 8 KW/cm2
wavelength 970 nm—Irradiance 15 KW/cm2
wavelength 1940 nm—Irradiance 2.5 KW/cm2
The viruses used were:
AAVs and lentiviruses were added in 1.5 mL Eppendorf tube (drop of 1 μL).
As shown in
Test No. 5—Test with IR Laser with Different Irradiances
Having found that the ideal wavelength is 1940 nm, the following tests served to calculate the minimum irradiance needed to destroy the viruses in 1 second.
Referring to
The viruses used were (recovery of small drops around the Eppendorf tube using the cellular medium):
In all three cases the complete destruction of the viruses was achieved.
Test No. 6—Further Tests with IR Laser with Different Irradiances
Referring to
The viruses used were (recovery of small drops around the Eppendorf tube using the cellular medium):
The results of the test show that with a laser at 1940 nm the destructive effect of the virus is obtained and with safety up to an irradiance of only 1.9 W/cm2 in just one second of exposure.
Test No. 7—Further Tests with IR Laser at 10600 nm
Referring to
Test No. 8—Test with Lentiviral Vector in Aerosol
The GFP lentiviral vector in solution was nebulized, collected, and plated with the cells. The aerosol was treated for 15 msec with the device according to the invention (in which there was only one laser beam) before being added to the cells. In the left figures, the presence of biologically active virus in the aerosol is demonstrated by the massive positivity of the cells to GFP. The antiviral effect of the laser according to the invention on the GFP lentiviral vector is shown in the right panel, in which very few cells test positive for infection.
Test No. 9—Test with Legionella in Aerosol
A sample of Legionella pneumophila in solution was nebulized, collected, and placed on Petri dishes. The generated aerosol was treated for 15 msec with a prototype of the device of the invention (treated) and untreated (untreated—CTRL). It should be specified here that only one laser beam was used in the prototype, but more laser beams can be used (therefore with a longer exposure time) to increase the efficacy of the sterilization. This consideration is valid for any type of sterilization, including those of the other tests reported in the present description.
As shown in the leftmost images, the presence of viable bacteria in the aerosol is demonstrated by the formation of actively growing colonies on the slides, especially the untreated ones (CTRL). The antimicrobial effect of the laser of the device according to the invention on Legionella pneumophila is shown on the lower dishes (dishes with treated material), in which only a few colonies are visible. The total count of live Legionella pneumophila cells after treatment with the device of the invention is also shown in the figure. The number of live Legionella pneumophila bacteria is significantly reduced by three orders of magnitude.
Test No. 10—Test with Sars-Cov-2 in Aerosol
Tests were carried out to verify the antiviral effect of the device according to the invention on Sars-CoV-2 viruses in aerosols.
The quantification of the viruses was performed 72 hours after the infection and the laser treatment according to the invention. Sars-CoV-2 virus in solution was nebulized, collected, and deposited on plates on primate Vero cells. The generated aerosol was treated for 15 msec with a prototype of the device according to the invention and compared with an untreated control sample. While the untreated sample showed a PFU/ml amount of 5.5×103, the treated sample showed a reduced PFU/ml amount of 7×102, therefore with an 87% reduction of the virus in just 15 ms of treatment.
It should also be specified here that only one laser beam was used in the prototype, which is already very effective, but more laser beams can be used (therefore with a longer exposure time) to increase the efficacy of the sterilization.
Referring to
Basically, in the device 300 the central body 310 is the chamber in which the laser grid 315 is developed as described above. Means 360, 370 are placed at the inlet and/or outlet of the central body 310 for restricting the volume towards the respective mouths of the central body, so that the volume increases away from the central body. This causes a Venturi effect, i.e., an acceleration of the airflow (large arrows) inside the central body with a simultaneous increase in pressure in the ducts 360 and/or 370. The increase in pressure was found to affect the survival of viruses, while it is less relevant for that of bacteria. At the outlet of the device according to the invention, after the enlargement 370 a conduit 380 with substantially constant section can be placed. The device 300 can be multiplied, i.e., multiple devices 300 can be connected in a row to increase the effects on sterilization.
A variation of such an embodiment is shown in
The inventor deduces that the increased pressure is able to destroy the viral materials, known to be weak, with particular synergy and efficacy if the laser is used as described. The materials weakened by one are completely destroyed by the other, regardless of the order of application.
The present invention consists of a device which adopts a sustainable architecture, through innovative design and technology which allow it to be optimized in multifunctional use, providing for example the following advantages:
In no case of the prior art air was subjected to great laser light exposure energy for a stable time ≥500 ms, as can instead be achieved by the present invention.
In addition to the advantages listed above, the installation space is recovered, and can be used for multiple functions, including contemporary ones, such as:
The device according to the invention can destroy any virus or bacterium or insect, and in particular among the viruses:
The present invention is not only capable of destroying the virus in just one second, but can be used with maximum safety and without contraindications, precisely in case of presence of people, in any space by aspirating aerosol and sterilizing it in only one second. Therefore, it finds application in all spaces, being fixed or movable, such as any means of transport, buses, ships, airplanes, etc.
It does not produce any polluting material for disposal and requires maintenance every 24 months. It may be transported with supports provided with wheels, hung from the ceiling, either leaned or hung on the wall, and in any dynamic position. It does not require special technical installations, simply a connection to a universal low-voltage electricity source: 100-240 VAC 50/60 Hz. It consumes about 100 W/h depending on the size of the model to be installed.
According to an aspect of the invention, the device described and claimed is capable of measuring the concentration of droplets present in the space where the device is installed and the proximity or presence and concentration of people present in the space by means of suitable sensors. This allows the device to activate dynamically, adjusting the action intensity thereof in a very dynamic manner, saving energy and lengthening the lifetime of the wearable parts. Due to this “smart” use, the electric power absorbed varies from a few tens of watts to peaks of 600 W.
The preferred embodiments and possible variants of the present invention have been outlined above, but it is to be understood that those skilled in the art may make modifications and changes without thereby departing from the scope of protection, as defined in the appended claims.
| Number | Date | Country | Kind |
|---|---|---|---|
| 102020000015841 | Jul 2020 | IT | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/IB2021/055539 | 6/23/2021 | WO |
