Claims
- 1. An apparatus kit for detecting Dientamoeba fragilis in a sample including feces or intestinal mucosa, comprising:
- a centrifugable sample vessel for holding said sample and having therein at least a quantity of isotonic solution, said vessel presenting converging sidewalls defining a region having sediment containing Dientamoeba fragilis and residual material derived from centrifugation of said sample therein;
- a quantity of a staining agent means comprising an acridine compound for contacting and staining said sediment in said sample vessel, said quantity of staining agent means in said kit being present in a container selected from the group consisting of said sample vessel and a staining agent vessel separate from said sample vessel,
- said sample vessel having a sufficient volume to simultaneously accommodate said isotonic solution, said staining agent means and said sediment therein,
- said quantity of staining agent means causing said Dientamoeba fragilis in said sediment to stain a first color and said residual material of the sediment to stain a second color; and
- means for viewing a portion of said stained sediment through a fluorescent microscope for detecting said first color as an indication of the presence of the Dientamoeba fragilis in said sample.
- 2. The apparatus kit of claim 1, said staining agent means being present in said staining agent vessel.
- 3. The apparatus kit of claim 1, said isotonic solution and staining agent means being present in said sample vessel.
- 4. The apparatus kit of claim 1, including swab means for transferring a sample into said sample vessel.
- 5. The apparatus kit of claim 1, said isotonic solution comprising an aqueous salt solution having an osmolality from about 200-400 milliOsm/l.
- 6. The apparatus kit of claim 1, said isotonic solution further including a pH indicator.
- 7. The apparatus kit of claim 1, said staining agent means including an acridine compound selected from the group consisting of acridine orange, acridine orange base, acridine yellow and mixtures thereof.
- 8. The apparatus kit of claim 1, said staining agent means further including a fixative.
- 9. The apparatus kit of claim 1, said staining agent means further including a preservative.
- 10. The apparatus kit of claim 1, said acridine compound being present at a level of from about 0.4 micrograms-5 milligrams per milliliter when said isotonic solution and staining agent means are present together in said sample vessel.
Parent Case Info
This application is a divisional of application Ser. No. 07/964,874, filed Oct. 22, 1992 now U.S. Pat. No. 5,334,509, issued Aug. 2, 1994.
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Date |
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3684377 |
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|
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|
4376820 |
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Non-Patent Literature Citations (3)
Entry |
Diagnostic Parasitology, Clinical Laboratory Manual, 2nd edition, 1979, pp. 99-101 and 105. |
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Divisions (1)
|
Number |
Date |
Country |
Parent |
964874 |
Oct 1992 |
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