Patent Application
20230263748
The present invention relates to the field of biomedicine, in particular to the application of benflumetol and derivatives thereof in the treatment of coronavirus infection.
Lumefantrine/benflumetol is the main active ingredient of the marketed drug compound artemether-benflumetol tablets (trade name: Coartem/Riamet), which has good antimalarial activity. Compound benflumetol has been approved for the treatment of patients weighing more than 5 kg (including 5 kg) with acute simple infection caused by Plasmodium falciparum. At the same time, compound benflumetol also has an ideal therapeutic effect on patients infected with falciparum malaria in chloroquine-resistant areas. Compound artemether-benflumetol tablets (Artemether-Lumefantrine) contain 20 mg of artemether and 120 mg of benflumetol. The two are used together as the main active ingredients, which helps to complement each other's antimalarial effects, and show significant therapeutic effects on Plasmodium falciparum and diseases thereof. In addition to the use in malaria treatment, benflumetol has strong antitumor activity, which can effectively inhibit the growth of prototype and drug-resistant glioblastoma in in vitro and in vivo models. In addition, the results of clinical trials show that compound benflumetol may have a certain inhibitory effect on human cytomegalovirus. At present, compound artemether-benflumetol tablets have been approved for marketing in China, the United States, Switzerland and other countries around the world.
The 2019 novel coronavirus (2019-nCoV) is a new strain of coronavirus that has never been found in humans before. On Feb. 11, 2020, the International Committee on Taxonomy of Viruses (ICTV) announced that the official classification of the 2019 novel coronavirus (2019-nCoV) is severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). On the same day, the World Health Organization (WHO) announced that the official name of the disease caused by the virus is COVID-19. The symptoms of SARS-CoV-2 infection are mainly pneumonia, which can be divided into simple infection, mild pneumonia, severe pneumonia, acute respiratory distress syndrome, sepsis, and septic shock according to the severity of the disease. Patients with simple infection may have nonspecific symptoms such as fever, cough, sore throat, nasal congestion, fatigue, headache, muscle pain or discomfort, and elderly and immunosuppressed individuals may experience atypical symptoms. Patients with mild pneumonia mainly have symptoms of cough, dyspnea and tachypnea. Severe pneumonia can be seen in adolescents, adults, or children. And the main symptoms of severe pneumonia are increased respiratory rate, severe respiratory failure or dyspnea, central cyanosis, lethargy, unconsciousness or convulsions, and gasp, etc. The lung images of acute respiratory distress syndrome are bilateral ground-glass opacities, which cannot be completely explained by effusion, lobar exudation, atelectasis, or pulmonary mass. And the main symptom of acute respiratory distress syndrome is pulmonary edema. Patients with sepsis often have fatal organ dysfunction, and patients with septic shock are the most critical patients with a high probability of death. At present, for the new coronavirus infection, supportive treatment is mainly used in clinic, and no specific antiviral drug is available.
The purpose of the present invention is to find a drug with antiviral activity against coronavirus, especially SARS-CoV-2, which can be used for a related disease caused by the infection thereof, for example simple infection such as fever, cough and sore throat, pneumonia, acute or severe acute respiratory infection, hypoxic respiratory failure and acute respiratory distress syndrome, sepsis and septic shock, etc. Through creative research, the present invention finds that the compound represented by Formula A (benflumetol and derivative thereof) has the function of inhibiting the replication of SARS-CoV-2, and has a good potential therapeutic effect in the treatment of a disease caused by SARS-CoV-2.
To this end, in the first aspect of the present invention, the present invention provides use of the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof in the manufacture of a medicament for the treatment of a disease or infection caused by a coronavirus (preferably SARS-CoV-2);
The inventors found that the compound represented by Formula A can inhibit viral replication on cells and reduce the viral nucleic acid load in cell culture.
In some embodiments, the disease caused by SARS-CoV-2 is COVID-19.
In some embodiments, the disease or infection caused by SARS-CoV-2 is a respiratory disease, such as simple infection, mild pneumonia, severe pneumonia, acute respiratory infection, severe acute respiratory infection (SARI), hypoxic respiratory failure, acute respiratory distress syndrome, sepsis, septic shock, etc.
In some embodiments, the simple infection includes, but is not limited to, fever, cough, sore throat, nasal congestion, fatigue, headache, muscle pain or discomfort. In some embodiments, the mild pneumonia includes, but is not limited to, cough, dyspnea, and/or tachypnea. In some embodiments, the severe pneumonia includes, but is not limited to, increased respiratory rate, severe respiratory failure or dyspnea, central cyanosis, lethargy, unconsciousness or convulsions, and gasp. In some embodiments, the acute respiratory distress syndrome includes, but is not limited to, pulmonary edema. In some embodiments, the sepsis includes, but is not limited to, organ dysfunction.
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, the structure of the compound of Formula A is represented by Formula I,
In the second aspect of the present invention, the present invention provides use of a pharmaceutical composition in the manufacture of a medicament for treating a disease or infection caused by a coronavirus (preferably SARS-CoV-2), wherein the pharmaceutical composition comprises the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof, and the compound represented by Formula A is as described above.
In some embodiments, the disease caused by SARS-CoV-2 is COVID-19.
In some embodiments, the disease or infection caused by SARS-CoV-2 is a respiratory disease, such as simple infection, mild pneumonia, severe pneumonia, acute respiratory infection, severe acute respiratory infection (SARI), hypoxic respiratory failure, acute respiratory distress syndrome, sepsis, septic shock, etc.
In some embodiments, the simple infection includes, but is not limited to, fever, cough, sore throat, nasal congestion, fatigue, headache, muscle pain or discomfort. In some embodiments, the mild pneumonia includes, but is not limited to, cough, dyspnea, and/or tachypnea. In some embodiments, the severe pneumonia includes, but is not limited to, increased respiratory rate, severe respiratory failure or dyspnea, central cyanosis, lethargy, unconsciousness or convulsions, and gasp. In some embodiments, the acute respiratory distress syndrome includes, but is not limited to, pulmonary edema. In some embodiments, the sepsis includes, but is not limited to, organ dysfunction.
In some embodiments, the pharmaceutical composition further comprises one or more selected from the group consisting of arteether, artemether, artemisone, dihydroartemisinin, artesunate, arteannuin B, artemisinic acid, and artemisinin. In some preferred embodiments, the pharmaceutical composition further comprises artemether, for example, it can be a compound artemether-benflumetol tablet.
In some embodiments, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient, specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
In the third aspect of the present invention, the present invention provides the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof, for use in the treatment of a disease or infection caused by a coronavirus (preferably SARS-CoV-2);
In some embodiments, the disease caused by SARS-CoV-2 is COVID-19.
In some embodiments, the disease or infection caused by SARS-CoV-2 is a respiratory disease, such as simple infection, mild pneumonia, severe pneumonia, acute respiratory infection, severe acute respiratory infection (SARI), hypoxic respiratory failure, acute respiratory distress syndrome, sepsis, septic shock, etc.
In some embodiments, the simple infection includes, but is not limited to, fever, cough, sore throat, nasal congestion, fatigue, headache, muscle pain or discomfort. In some embodiments, the mild pneumonia includes, but is not limited to, cough, dyspnea, and/or tachypnea. In some embodiments, the severe pneumonia includes, but is not limited to, increased respiratory rate, severe respiratory failure or dyspnea, central cyanosis, lethargy, unconsciousness or convulsions, and gasp. In some embodiments, the acute respiratory distress syndrome includes, but is not limited to, pulmonary edema. In some embodiments, the sepsis includes, but is not limited to, organ dysfunction.
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, the structure of the compound of Formula A is represented by Formula I,
In the fourth aspect of the present invention, the present invention provides a pharmaceutical composition, for use in treating a disease or infection caused by a coronavirus (preferably SARS-CoV-2), wherein the pharmaceutical composition comprises the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof, and the compound represented by Formula A is as described above.
In some embodiments, the disease caused by SARS-CoV-2 is COVID-19.
In some embodiments, the disease or infection caused by SARS-CoV-2 is a respiratory disease, such as simple infection, mild pneumonia, severe pneumonia, acute respiratory infection, severe acute respiratory infection (SARI), hypoxic respiratory failure, acute respiratory distress syndrome, sepsis, septic shock, etc.
In some embodiments, the simple infection includes, but is not limited to, fever, cough, sore throat, nasal congestion, fatigue, headache, muscle pain or discomfort. In some embodiments, the mild pneumonia includes, but is not limited to, cough, dyspnea, and/or tachypnea. In some embodiments, the severe pneumonia includes, but is not limited to, increased respiratory rate, severe respiratory failure or dyspnea, central cyanosis, lethargy, unconsciousness or convulsions, and gasp. In some embodiments, the acute respiratory distress syndrome includes, but is not limited to, pulmonary edema. In some embodiments, the sepsis includes, but is not limited to, organ dysfunction.
In some embodiments, the pharmaceutical composition further comprises one or more selected from the group consisting of arteether, artemether, artemisone, dihydroartemisinin, artesunate, arteannuin B, artemisinic acid, and artemisinin. In some preferred embodiments, the pharmaceutical composition further comprises artemether, for example, it can be a compound artemether-benflumetol tablet.
In some embodiments, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient, specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
In the fifth aspect of the present invention, the present invention provides a method for treating a disease, which comprises administering to a subject in need thereof a therapeutically effective amount of the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof, the disease being a disease or infection caused by a coronavirus, preferably SARS-CoV-2,
In some embodiments, the disease caused by SARS-CoV-2 is COVID-19.
In some embodiments, the disease or infection caused by SARS-CoV-2 is a respiratory disease, such as simple infection, mild pneumonia, severe pneumonia, acute respiratory infection, severe acute respiratory infection (SARI), hypoxic respiratory failure, acute respiratory distress syndrome, sepsis, septic shock, etc.
In some embodiments, the simple infection includes, but is not limited to, fever, cough, sore throat, nasal congestion, fatigue, headache, muscle pain or discomfort. In some embodiments, the mild pneumonia includes, but is not limited to, cough, dyspnea, and/or tachypnea. In some embodiments, the severe pneumonia includes, but is not limited to, increased respiratory rate, severe respiratory failure or dyspnea, central cyanosis, lethargy, unconsciousness or convulsions, and gasp. In some embodiments, the acute respiratory distress syndrome includes, but is not limited to, pulmonary edema. In some embodiments, the sepsis includes, but is not limited to, organ dysfunction.
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, the structure of the compound of Formula A is represented by Formula I,
In the sixth aspect of the present invention, the present invention provides a method for treating a disease, comprising administering to a subject in need thereof, a therapeutically effective amount of a pharmaceutical composition, wherein the disease is a disease or infection caused by a coronavirus (preferably SARS-CoV-2), the pharmaceutical composition comprises the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof, and the compound represented by Formula A is as described above.
In some embodiments, the disease caused by SARS-CoV-2 is COVID-19.
In some embodiments, the disease or infection caused by SARS-CoV-2 is a respiratory disease, such as simple infection, mild pneumonia, severe pneumonia, acute respiratory infection, severe acute respiratory infection (SARI), hypoxic respiratory failure, acute respiratory distress syndrome, sepsis, septic shock, etc.
In some embodiments, the simple infection includes, but is not limited to, fever, cough, sore throat, nasal congestion, fatigue, headache, muscle pain or discomfort. In some embodiments, the mild pneumonia includes, but is not limited to, cough, dyspnea, and/or tachypnea. In some embodiments, the severe pneumonia includes, but is not limited to, increased respiratory rate, severe respiratory failure or dyspnea, central cyanosis, lethargy, unconsciousness or convulsions, and gasp. In some embodiments, the acute respiratory distress syndrome includes, but is not limited to, pulmonary edema. In some embodiments, the sepsis includes, but is not limited to, organ dysfunction.
In some embodiments, the pharmaceutical composition further comprises one or more selected from the group consisting of arteether, artemether, artemisone, dihydroartemisinin, artesunate, arteannuin B, artemisinic acid, and artemisinin. In some preferred embodiments, the pharmaceutical composition further comprises artemether, for example, it can be a compound artemether-benflumetol tablet.
In some embodiments, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient, specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
In the seventh aspect of the present invention, the present invention provides use of the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof in the manufacture of a medicament as an inhibitor of a coronavirus (preferably SARS-CoV-2),
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, the structure of the compound of Formula A is represented by Formula I
In the eighth aspect of the present invention, the present invention provides use of a pharmaceutical composition in the manufacture of a medicament as an inhibitor of a coronavirus (preferably SARS-CoV-2), wherein the pharmaceutical composition comprises the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof, and the compound represented by Formula A is as described above.
In some embodiments, the pharmaceutical composition further comprises one or more selected from the group consisting of arteether, artemether, artemisone, dihydroartemisinin, artesunate, arteannuin B, artemisinic acid, and artemisinin. In some preferred embodiments, the pharmaceutical composition further comprises artemether, for example, it can be a compound artemether-benflumetol tablet.
In some embodiments, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient, specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
In the ninth aspect of the present invention, the present invention provides the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof, which is an inhibitor of a coronavirus (preferably SARS-CoV-2),
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, the structure of the compound of Formula A is represented by Formula I,
In the tenth aspect of the present invention, the present invention provides a pharmaceutical composition, which is an inhibitor of a coronavirus (preferably SARS-CoV-2), wherein the pharmaceutical composition comprises the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof, and the compound represented by Formula A is as described above.
In some embodiments, the pharmaceutical composition further comprises one or more selected from the group consisting of arteether, artemether, artemisone, dihydroartemisinin, artesunate, arteannuin B, artemisinic acid, and artemisinin. In some preferred embodiments, the pharmaceutical composition further comprises artemether, for example, it can be a compound artemether-benflumetol tablet.
In some embodiments, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient, specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
In the eleventh aspect of the present invention, the present invention provides use of the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof in the manufacture of a medicament for inhibiting the replication or proliferation of a coronavirus (preferably SARS-CoV-2) in a cell (e.g., mammalian cell);
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, the structure of the compound of Formula A is represented by Formula I,
In the twelfth aspect of the present invention, the present invention provides use of a pharmaceutical composition in the manufacture of a medicament for inhibiting the replication or proliferation of a coronavirus (preferably SARS-CoV-2) in a cell (e.g., mammalian cell), wherein the pharmaceutical composition comprises the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof, and the compound represented by Formula A is as described above.
In some embodiments, the pharmaceutical composition further comprises one or more selected from the group consisting of arteether, artemether, artemisone, dihydroartemisinin, artesunate, arteannuin B, artemisinic acid, and artemisinin. In some preferred embodiments, the pharmaceutical composition further comprises artemether, for example, it can be a compound artemether-benflumetol tablet.
In some embodiments, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient, specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
In the thirteenth aspect of the present invention, the present invention provides the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof, for use in inhibiting the replication or proliferation of a coronavirus (preferably SARS-CoV-2) in a cell (e.g., mammalian cell);
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, the structure of the compound of Formula A is represented by Formula I,
In the fourteenth aspect of the present invention, the present invention provides a pharmaceutical composition for use in inhibiting the replication or proliferation of a coronavirus (preferably SARS-CoV-2) in a cell (e.g., mammalian cell), wherein the pharmaceutical composition comprises the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof, the compound represented by Formula A is as described above.
In some embodiments, the pharmaceutical composition further comprises one or more selected from the group consisting of arteether, artemether, artemisone, dihydroartemisinin, artesunate, arteannuin B, artemisinic acid, and artemisinin. In some preferred embodiments, the pharmaceutical composition further comprises artemether, for example, it can be a compound artemether-benflumetol tablet.
In some embodiments, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient, specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
In the fifteenth aspect of the present invention, the present invention provides a method for inhibiting the replication or proliferation of a coronavirus (preferably SARS-CoV-2) in a cell (e.g., mammalian cell), comprising administering to the cell (e.g., mammalian cell) an effective amount of the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof;
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, Ra, Rb are each independently selected from the group consisting of n-butyl, isobutyl, tert-butyl, sec-butyl.
In some embodiments, the structure of the compound of Formula A is represented by Formula I,
In the sixteenth aspect of the present invention, the present invention provides a method for inhibiting the replication or proliferation of a coronavirus (preferably SARS-CoV-2) in a cell (e.g., mammalian cell), comprising administering to the cell (e.g., mammalian cell) an effective amount of a pharmaceutical composition, wherein the pharmaceutical composition comprises the compound represented by Formula A, or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof, and the compound represented by Formula A is as described above.
In some embodiments, the pharmaceutical composition further comprises one or more selected from the group consisting of arteether, artemether, artemisone, dihydroartemisinin, artesunate, arteannuin B, artemisinic acid, and artemisinin. In some preferred embodiments, the pharmaceutical composition further comprises artemether, for example, it can be a compound artemether-benflumetol tablet.
In some embodiments, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient, specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
In some embodiments, the mammal includes bovine, equine, ovine, porcine, canine, feline, rodent, primate, and for example, is a human, cat, dog or pig.
In the present invention, the official classification name of the term “2019 novel coronavirus (2019-nCoV)” is severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
In the present invention, the official name of the term “disease caused by 2019 novel coronavirus (2019-nCoV)” is COVID-19.
In the present invention, “subject” refers to a vertebrate. In certain embodiments, the vertebrate refers to a mammal. The mammal includes bovine, equine, ovine, porcine, canine, feline, rodent, primate, and for example is a human, cat, dog or pig. The mammal includes, but is not limited to, livestock (e.g., cattle), pet (e.g., cat, dog, and horse), primate, mouse, and rat. In certain embodiments, the mammal refers to a human.
In the present invention, the term “therapeutically effective amount” or “prophylactically effective amount” refers to an amount sufficient to treat or prevent a patient's disease but low enough to avoid serious side effects (at a reasonable benefit/risk ratio) within the scope of sound medical judgment. The therapeutically effective amount of a compound will depend on the particular compound selected (e.g., taking into account the potency, effectiveness and half-life of the compound), the route of administration selected, the disease being treated, the severity of the disease being treated, the age, size, weight and physical ailment of the patient being treated, the medical history of the patient being treated, the duration of treatment, the nature of concurrent therapy, the desired therapeutic effect, and the like, but can still be routinely determined by those skilled in the art.
In addition, it should be pointed out that the specific dosage and usage of the compound represented by the Formula A, or its stereoisomer, or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate for different patients are determined by many factors, including the patient's age, weight, gender, natural health status, nutritional status, the active strength of drug, the time of administration, metabolic rate, the severity of condition, and the subjective judgment of the attending physician. It is preferred here to use a dose between 0.001 and 1000 mg/kg body weight/day.
The pharmaceutically acceptable salt of the compound represented by the Formula A of the present invention includes its inorganic or organic acid salt and its inorganic or organic base salt, and the present invention relates to all forms of the above-mentioned salts, including but not limited to: sodium salt, potassium salt, calcium salt, lithium salt, meglumine salt, hydrochloride, hydrobromide, hydroiodide, nitrate, sulfate, hydrosulfate, phosphate, hydrophosphate, acetate, propionate, butyrate, oxalate, trimethylacetate, adipate, alginate, lactate, citrate, tartrate, succinate, maleate, fumarate, picrate, aspartate, gluconate, benzoate, mesylate, ethanesulfonate, benzenesulfonate, p-toluenesulfonate and pamoate, etc.
The pharmaceutical compositions involved in the present invention may comprise a pharmaceutically acceptable carrier, including but not limited to: ion exchanger, alumina, aluminum stearate, lecithin, serum protein such as human serum albumin, buffer substance such as phosphate, glycerol, sorbic acid, potassium sorbate, partial glyceride mixture of saturated vegetable fatty acids, water, salt or electrolyte such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salt, colloidal silica, magnesium tri silicate, polyvinylpyrrolidone, cellulosic substance, polyethylene glycol, sodium carboxymethylcellulose, polyacrylate, beeswax, lanolin.
The pharmaceutical composition of the present invention can be prepared into various forms according to different administration routes.
According to the present invention, the pharmaceutical composition can be administered in any of the following ways: oral administration, spray inhalation, rectal administration, nasal administration, buccal administration, vaginal administration, topical administration, parenteral administration such as subcutaneous, intravenous, intramuscular, intraperitoneal, intrathecal, intraventricular, intrasternal and intracranial injection or infusion, or via an explanted reservoir. Of these, oral, intraperitoneal or intravenous administration is preferred.
For oral administration, the compound represented by Formula A, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate can be made into any orally acceptable preparation, including but not limited to tablet, capsule, aqueous solution or aqueous suspension. Among them, the commonly used carrier for tablet includes lactose and corn starch, and lubricant such as magnesium stearate may also be added. Commonly used diluent for capsule preparation includes lactose and dried cornstarch. Aqueous suspension is usually prepared by mixing the active ingredient with suitable emulsifying agent and suspending agent. If desired, some sweetening, flavoring or coloring agents may also be added to the above oral preparations.
For rectal administration, the compound represented by Formula A, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate can generally be made into the form of suppository, which is prepared by mixing the drug with a suitable non-irritating excipient. The excipient is solid at room temperature, but melts at rectal temperature to release the drug. Such excipient includes cocoa butter, beeswax and polyethylene glycol.
For topical administration, especially when treating affected surfaces or organs easily accessible by topical administration, such as eye, skin or lower intestinal neurological diseases, the compound represented by Formula A, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate can be made into different topical preparations according to different affected surfaces or organs, and the specific instructions are as follows:
For topical administration to the eye, the compound of Formula A, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate can be formulated into the form of a micronized suspension or solution, the carrier used is isotonic sterile saline with a certain pH, which may or may not be added with a preservative such as benzyl alkoxide chloride. In addition, for ophthalmic use, the compound can be formulated into the form of an ointment such as petrolatum ointment.
For topical administration to the skin, the compound represented by Formula A, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate can be made into the form of a suitable ointment, lotion or cream, in which the active ingredient is suspended or dissolved in one or more carriers. The carriers that can be used in ointment here include, but are not limited to: mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyethylene oxide, polypropylene oxide, emulsified wax and water; the carriers that can be used in lotion or cream include, but are not limited to: mineral oil, sorbitan monostearate, Tween 60, cetyl ester wax, cetenylaryl alcohol, 2-octyldodecanol, benzyl alcohol and water.
For topical administration to the lower intestinal tract, the compound represented by the Formula A, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate can be made into the above-mentioned rectal suppository preparation or suitable form of enema preparation. In addition, topical transdermal patches can also be used.
The compound represented by the Formula A, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate can also be administered in the form of sterile injectable preparation, including sterile injectable water or oil suspension, or sterile injectable solution. Among them, the carriers and solvents that can be used are water, Ringer's solution and isotonic sodium chloride solution. In addition, sterile nonvolatile oil such as monoglyceride or diglyceride can also be used as a solvent or suspending medium.
The medicaments in the above-mentioned various dosage forms can be prepared according to the conventional methods in the pharmaceutical field.
In various parts of this specification, the substituents of the compounds disclosed in the present invention are disclosed in terms of group type or range. Specifically, the present invention includes each and every independent subcombination of each member of these group types and ranges. For example, the term “C1-C6 alkyl” specifically refers to independently disclosed methyl, ethyl, C3 alkyl, C4 alkyl, C5 alkyl and C6 alkyl groups.
In addition, it should be noted that, unless it is clearly stated otherwise, the expression “each independently . . . ” used herein should be understood in a broad sense, which refers to that the specific options represented by different symbols do not affect each other, and can be the same or different.
The term “C1-C6 alkyl” refers to any straight or branched chain group containing 1 to 6 carbon atoms, such as methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, sec-butyl, n-pentyl, tert-amyl, n-hexyl, etc.
The examples of the present invention will be described in detail below. The examples described below with reference to the accompanying drawings are exemplary, and are intended to explain the present invention, but should not be construed as a limitation of the present invention.
The present invention provides a compound having the structure of Formula I, its stereoisomer, its pharmaceutically acceptable salt and/or its solvate and/or its hydrate:
In certain embodiments, the pharmaceutically acceptable salt of the compound of Formula I of the present invention includes its inorganic or organic acid salts, and inorganic or organic base salts, and the present invention relates to all forms of the above-mentioned salts, including but not limited to: sodium salt, potassium salt, calcium salt, lithium salt, meglumine salt, hydrochloride, hydrobromide, hydroiodide, nitrate, sulfate, hydrosulfate, phosphate, hydrophosphate, acetate, propionate, butyrate, oxalate, trimethylacetate, adipate, alginate, lactate, citrate, tartrate, succinate, maleate, fumarate, picrate, aspartate, gluconate, benzoate, mesylate, ethanesulfonate, benzenesulfonate, p-toluenesulfonate and pamoate, etc.
The compound of Formula I can inhibit viral replication on cells and reduce viral nucleic acid load in a cell culture.
The present invention relates to use of the compound represented by Formula I, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate in the manufacture of a medicament for the treatment of a disease or infection caused by a coronavirus, especially SARS-CoV-2 (including but not limited to respiratory disease (e.g., simple infection such as fever, cough and sore throat, etc., pneumonia, acute or severe acute respiratory infection, hypoxic respiratory failure and acute respiratory distress syndrome, sepsis and septic shock, severe acute respiratory syndrome, etc.)),
The present invention also relates to use of the compound represented by Formula I, its stereoisomer, its pharmaceutically acceptable salt and/or its solvate and/or its hydrate in the manufacture of a medicament as a coronavirus inhibitor.
The present invention also relates to use of the compound represented by Formula I, its stereoisomer, its pharmaceutically acceptable salt and/or its solvate and/or its hydrate in the manufacture of a medicament for inhibiting the replication or proliferation of a coronavirus in a cell (e.g., mammalian cell).
The present invention also relates to a pharmaceutical composition, which comprises the compound represented by the Formula I, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate, preferably, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient, specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
The present invention also relates to use of the pharmaceutical composition comprising the compound represented by the Formula I, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate, or the compound represented by the Formula I, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate, in the manufacture of a medicament for the treatment of a disease including respiratory disease but not limited to respiratory disease (including simple infection such as fever, cough and sore throat, etc., pneumonia, acute or severe acute respiratory infection, hypoxic respiratory failure and acute respiratory distress syndrome, sepsis and septic shock, severe acute respiratory syndrome, etc.).
The present invention also relates to a method for treating and/or preventing a disease in a mammal in need thereof or a method for inhibiting the replication or proliferation of a coronavirus in a mammal in need thereof, the method comprises administering to the mammal in need a therapeutically and/or prophylactically effective amount of the pharmaceutical composition comprising the compound represented by the Formula I, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate, or the compound represented by the Formula I, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate, wherein the disease includes a disease caused by a coronavirus.
In certain embodiments, the disease caused by a coronavirus, especially SARS-CoV-2, includes, but is not limited to, respiratory disease (e.g., simple infection such as fever, cough and sore throat, etc., pneumonia, acute or severe acute respiratory infection, hypoxic respiratory failure and acute respiratory distress syndrome, sepsis and septic shock, severe acute respiratory syndrome, etc.).
The present invention also relates to use of a pharmaceutical composition in the manufacture of a medicament for the treatment of a disease or infection caused by a coronavirus, especially SARS-CoV-2 (e.g., respiratory disease (e.g., simple infection such as fever, cough and sore throat, etc., pneumonia, acute or severe acute respiratory infection, hypoxic respiratory failure and acute respiratory distress syndrome, sepsis and septic shock, severe acute respiratory syndrome, etc.), wherein the pharmaceutical composition comprises the compound represented by Formula I, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate,
Preferably, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient, specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
The present invention also relates to use of a pharmaceutical composition in the manufacture of a medicament as a coronavirus inhibitor, wherein the pharmaceutical composition comprises the compound represented by Formula I, its stereoisomer, its pharmaceutically acceptable salt and/or its solvate and/or its hydrate,
Preferably, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient, specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
The present invention also relates to use of a pharmaceutical composition in the manufacture of a medicament for inhibiting the replication or proliferation of a coronavirus in a cell (e.g., mammalian cell), wherein the pharmaceutical composition comprises the compound represented by Formula I, its stereoisomer, its pharmaceutically acceptable salt and/or its solvate and/or its hydrate,
Preferably, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient, specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
The present invention also relates to the compound represented by Formula I, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate, for use in the treatment of a disease or infection caused by a coronavirus, especially SARS-CoV-2 (including but not limited to respiratory disease (e.g., simple infection such as fever, cough and sore throat, etc., pneumonia, acute or severe acute respiratory infection, hypoxic respiratory failure and acute respiratory distress syndrome, sepsis and septic shock, severe acute respiratory syndrome, etc.)).
The present invention also relates to the compound represented by Formula I, its stereoisomer, its pharmaceutically acceptable salt and/or its solvate and/or its hydrate, which is used as a coronavirus inhibitor.
The present invention also relates to the compound represented by Formula I, its stereoisomer, its pharmaceutically acceptable salt and/or its solvate and/or its hydrate, for use in inhibiting the replication or proliferation of a coronavirus in a cell (e.g., mammalian cell).
The present invention also relates to a pharmaceutical composition for use in the treatment of a disease or infection caused by a coronavirus, especially SARS-CoV-2 (e.g., respiratory disease (e.g., simple infection such as fever, cough and sore throat, etc., pneumonia, acute or severe acute respiratory infection, hypoxic respiratory failure and acute respiratory distress syndrome, sepsis and septic shock, severe acute respiratory syndrome, etc.)), wherein the pharmaceutical composition comprises the compound represented by the Formula I, its stereoisomer or its pharmaceutically acceptable salt and/or its solvate and/or its hydrate,
Preferably, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient, specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
The present invention also relates to a pharmaceutical composition, which is used as a coronavirus inhibitor, wherein the pharmaceutical composition comprises the compound represented by Formula I, its stereoisomer, its pharmaceutically acceptable salt and/or its solvate and/or its hydrate,
Preferably, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient, specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
The present invention also relates to a pharmaceutical composition, for use in inhibiting the replication or proliferation of a coronavirus in a cell (e.g., mammalian cell), wherein the pharmaceutical composition comprises the compound represented by Formula I, its stereoisomer, its pharmaceutically acceptable salt and/or its solvate and/or its hydrate,
Preferably, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient, specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
In certain embodiments, the coronavirus of the present invention is SARS-CoV-2.
In certain embodiments, the disease caused by the coronavirus of the present invention is the disease caused by SARS-CoV-2, namely COVID-19.
In certain embodiments, the mammal of the present invention includes bovine, equine, ovine, porcine, canine, feline, rodent, primate, for example is a human, cat, dog or pig.
The present invention will be further explained below in conjunction with specific examples.
(1) Drug Treatment of Virus-Infected Cells
Vero E6 cells (purchased from ATCC, Cat. No. 1586) were inoculated into a 24-well plate and cultured for 24 hours; then virus infection was performed, specifically, SARS-CoV-2 (2019-nCoV) virus (nCoV-2019BetaCoV/Wuhan/WIV04/2019 strain, provided by Wuhan Institute of Virology, Chinese Academy of Sciences) was diluted to the corresponding concentration with 2% cell maintenance solution (formulation was: FBS (purchased from Gibco, Cat. No. 16000044) was added to MEM (purchased from Gibco, Cat. No. 10370021) at a volume ratio of 2% to form 2% cell maintenance solution), and then added to the 24-well plate so that each well had a virus amount of 100 TCID50. Next, benflumetol (purchased from Selleck, Cat. No. 53746) was diluted to corresponding concentrations with 2% cell maintenance solution and added to the corresponding wells, so that the final drug concentrations were 100 μM, 50 μM, 25 μM, 12.5 μM, 6.25 μM, and 3.13 μM, respectively, and then incubation was performed in a 37° C., 5% CO2 incubator for 48 h; while 2% cell maintenance solution without any test drug was added only in the cell control group.
RNA extraction kit was purchased from Qiagen, Cat. No. 74106. The consumables (spin column, RNase-free 2 mL collection tubes, etc.) and reagents (RLT, RW1, RPE, RNase-free water, etc.) involved in the following RNA extraction steps were all parts of the kit. The following extraction steps were all those recommended by the kit instructions.
(3) RNA Reverse Transcription
The reverse transcription kit (PrimeScript™ RT reagent Kit with gDNA Eraser, Cat. No. RR047Q) produced by TaKaRa Company was used for RNA reverse transcription. The steps were as follows.
(4) Real-Time PCR
Fluorescence quantitative PCR was used to detect the copy number per milliliter of the original virus solution.
The reaction system was mixed using TB Green Premix (Takara, Cat #RR820A), and the amplification reaction and reading were performed by a StepOne Plus Real-time PCR machine (brand: ABI). The number of copies per milliliter of the original virus solution was calculated. The steps were as follows:
Inhibition rate (%)=(number of RNA copies in drug treatment group)/(number of RNA copies in cell infection group)×100%
(5) Detection of Drug Cytotoxicity
The detection of drug cytotoxicity was performed by CCK-8 kit (Beoytime). Specific steps were as follows:
Cell viability (%)=(A(drug treatment group)−A(blank control))/(A(negative control)−A(blank control))×100%
(6) Experimental Results
The results of the virus proliferation inhibition experiment showed that the test compound could effectively inhibit the replication of the SARS-CoV-2 virus genome in the infection supernatant at the concentrations of 100 μM, 50 μM and 25 μM. (Table 1 and
The cytotoxicity results showed that the treatment of the test compound (benflumetol) did not change the cell viability at all test concentrations, that was, the test compound had no toxic effect on the cells at all concentrations (Table 2 and
The specific embodiments described above further describe the purpose, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above-mentioned specific embodiments are only specific embodiments of the present invention, and are not intended to limit the scope of the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
| Number | Date | Country | Kind |
|---|---|---|---|
| 202010722678.6 | Jul 2020 | CN | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/CN2021/107739 | 7/22/2021 | WO |
