This application is the national stage entry of International Application No. PCT/TR2020/050866, filed on Sep. 18, 2020, which is based upon and claims priority to Turkish Patent Application No. 2019/15068, filed on Oct. 2, 2019, the entire contents of which are incorporated herein by reference.
The invention is related to integrating an aquaporin protein addition to the outer surface of hollow fiber membranes and the production method of aquaporin Z integrated membrane using different hollow fiber support membranes comprising polymeric, nanocomposite materials as hollow fiber membranes.
The production of thin film composite membranes (TFC) using interfacial polymerization has been developed by Cadotte et al., in the 1970's. A selective layer (varies between 20-200 nm) that enables to separate the porous membrane that acts as support is available in TFC membranes. These membranes express superior selectivity, permeability and mechanical strength properties. Amine and acyl chloride monomers are dissolved in water and in an organic phase and are subjected to reaction in order to produce these membranes with interfacial polymerization and a polyamide layer is formed on the interfacial surface of the reaction. By means of this polyamide layer, water purification is achieved at nanofiltration levels.
Nowadays reverse osmosis membranes have approximately 1-2 L/m2·h·bar permeability and % 99.6 salt rejection. However since high pressure needs to be applied, energy consumptions are around 2 kW·s/m3 and this increases the processing costs of membranes. Due to this reason it is important to produce membranes having high flux and selectivity.
The concept of producing membranes doped with aquaporin was initially conveyed in 2007 by Kumar et al., (2). Since that day, membranes have been processed for nanofiltration, reverse osmosis and forward osmosis processes using flat sheet membranes.
In Table I, the permeability comparison of TO and FO membranes against membranes produced using Aquaporin has been given.
Various improvements have been carried out in the prior art, in relation to the production method of aquaporin Z integrated membranes using fiber reinforced membranes.
In the Canadian patent document numbered CA2607371C of the prior art, membranes comprising functional water channels inside lipids having structures similar to cell membranes have been disclosed. The lipid bilayers have been arranged as sandwich structures comprising hydrophilic or hydrophobic support materials. Water purification devices/systems including reverse osmosis filtering devices comprising membranes with functional aquaporins are also described in the document. Moreover the method of preparing water purification membranes is also described.
In the prior art Japanese patent document numbered JP2012192408A, membranes which are similar to sandwich structures and which comprise bi-layer lipids, hydrophilic or hydrophobic support materials are disclosed. Moreover, water purification membrane preparation methods including reverse osmosis filtering devices comprising membranes with functional aquaporins are also described. Additionally, the invention provides lipid membranes that comprise other transmembrane proteins besides aquaporins that comprise porous hydrophobic polymer films.
The Korean patent document numbered KR20140004365U of the prior art, is related to an aquaporin water channel that is equipped with hollow fibers having a fiber membrane modified with composite structures (TFC). The present invention additionally discloses a hollow fiber membrane that has a separation layer modified with a thin film composite (TFC), comprising a polyimide TFC layer therein, instead of fiber. It comprises a water channel that is embedded into a layer or immobilized as a method for directly forming TFC modification on fiber.
In the Canadian patent document numbered CA2897354C of the prior art, a hollow fiber (HF) module that has fibers modified with a thin film composite (TFC) layer comprising aquaporin water channels is mentioned.
When the methods present in the art are examined, it has been noted that an aquaporin Z integrated membrane production method was required, wherein aquaporin integrated membrane is provided by producing only polymeric, nanocomposite and reinforced hollow fibers, where doping of aquaporin to hollow fiber membranes is carried out using polysulfone polymer.
The aim of the invention is to provide a membrane production method where only polymeric, nanocomposite and reinforced hollow fibers are individually produced and where individual aquaporin doped membrane production is carried out.
Another aim of the invention is to provide a membrane production method where aquaporin integration is carried out on hollow fiber membranes using polysulfone polymer.
Another aim of the invention is to provide a membrane production method where the aquaporin protein is embedded into the liposome.
Another aim of the invention is to provide a membrane production method where membrane flux and strength is increased when aquaporin protein integration is carried out using hollow fiber membranes.
Another aim of the invention is to provide a membrane production method resistant under high pressure, where fluxes that are similar to hollow fiber nanofiltration membrane fluxes are obtained.
The production method of aquaporin Z integrated membrane using different hollow fiber support membranes carried out in order to reach the aims of this invention has been illustrated in the attached figures.
According to these figures;
Hollow fiber membrane is produced in order to be used as 3 different support layers in the membrane production method subject to the invention. The contents of the membrane dope solution for production is 16% polysulfone, 10% polyvinyl pyrrolidone (Molecular weight: 360 kDa (kilo Daltons)), 74% n-methyl pyrrolidone. 0.01% carbon nanotube (external diameter <8 nm) is used for the nanocomposite doped support layer. The contents of the membrane dope solution for the production of reinforced support layer is 16% polysulfone, 10% polyvinyl pyrrolidone (Molecular weight: 40 kDa (kilo Daltons)), 74% n-methyl pyrrolidone and polyester (PET) textile yarn.
Dioleoylphosphocholine (DOPC) lipid that was dissolved in chloroform for aquaporin protein doping was used and the chloroform that was present inside the solution having 0.1% DOPC concentration by weight has been evaporated in nitrogen medium. Following this 10 mM phosphate buffer solution (PBS) has been added into DOPC lipid. The liposomes have been produced by being vortexed with the rehydration method and 1% aquaporin by weight has been added into the liposomes. 1% dodecylmaltoside (DDM) detergent was added for the aquaporin protein to reconstitute into liposomes more effectively. Detergent added during the reconstitution process was removed by adding biobeads which are consisting of neutral, porous styrene divinylbenzene beads.
The solution has been extruded by a mini extruder by using polytetrafluoroethylene (PTFE) membranes having 200 nm pore size in order to reduce the liposomes that contain aquaporin Z to a similar size. In order to compare the performance of the purified protein, aquaporin protein that was purchased commercially was used.
Parameters that are used in the production of hollow fiber membranes have been given in Table II. An interfacial surface polymerization has been used in order to reconstitute the aquaporin protein into the 3 different support layer. 2% piperazine (PIP) was dissolved in water and 0.2% trimesoyl chloride (TMC) was dissolved in cyclohexane in order to carry our interfacial polymerization. The Aquaporin Z reconstituted DOPC liposomes were prepared at 0.1% concentration was added into the solution with piperazine. The hollow fiber membranes were first immersed into the PIP+Aquaporin including solution for 2 minutes. Following this the membranes were passed through 1 atm nitrogen gas in order to remove the non-reacted PIP monomers available on the surface from the membrane. After this, the membranes were left in 100% cyclohexane solution for 1 minute and they were then immersed into TMC solution and after they were kept here for 1 minute, they were kept in a drying oven for 5 minutes at 50° C.
The membrane area was increased from 26 cm2 to 280 cm2 and the water purification performance was evaluated from this membrane area by comparing both synthetic water and the water sample obtained from the lake water of Ömerli barrage. The results of this comparison were given in
When aquaporin protein integration is carried out using reinforced hollow fiber membranes in the invention, increase in the strength and flux of membranes was observed. As a result several benefits were gained. First of all by means of the Aquaporin addition, increase was observed in membrane flux and as a result lower pressure is applied to obtain flux similar to other hollow fiber nanofiltration membranes. Aquaporin protein integrated reinforced membranes increased resilience of membranes to higher pressures. This shows that the usage of membranes in processes that are subject to high pressure such as reverse osmosis and nanofiltration could be advantageous.
Number | Date | Country | Kind |
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2019/15068 | Oct 2019 | TR | national |
Filing Document | Filing Date | Country | Kind |
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PCT/TR2020/050866 | 9/18/2020 | WO |
Publishing Document | Publishing Date | Country | Kind |
---|---|---|---|
WO2021/066773 | 4/8/2021 | WO | A |
Number | Name | Date | Kind |
---|---|---|---|
4039440 | Cadotte | Aug 1977 | A |
20140332468 | Tang | Nov 2014 | A1 |
20150144553 | Vogel | May 2015 | A1 |
20150273407 | Gil | Oct 2015 | A1 |
Number | Date | Country |
---|---|---|
2607371 | Apr 2016 | CA |
2897354 | Mar 2019 | CA |
105624183 | Jun 2016 | CN |
105624183 | Jun 2016 | CN |
2012192408 | Oct 2012 | JP |
20140004365 | Jan 2014 | KR |
20180078165 | Jul 2018 | KR |
2012161662 | Nov 2012 | WO |
2014063097 | Apr 2014 | WO |
WO-2017030502 | Feb 2017 | WO |
WO-2018182518 | Oct 2018 | WO |
Entry |
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Enise Pekgenc, Aquaporin Z Embedded Flat Sheet Nanofiltration Membranes Production, Characterization and Removal of Organic Matter, Jun. 2018. (Year: 2018). |
Manish Kumar, et al., Highly permeable polymeric membranes based on the incorporation of the functional water channel protein Aquaporin Z, PNAS, 2007, pp. 20719-20724, vol. 104 No. 52. |
Enise Pekgenc, Aquaporin Z Embedded Flat Sheet Nanofiltration Membranes Production, Characterization and Removal of Organic Matter, 2018. |
Rastislav Varhac, et al., Removal of bound Triton X-100 from purified bovine heart cytochrome bc1, Analytical Biochemistry, 2009, pp. 268-270, vol. 395. |
Number | Date | Country | |
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20220274068 A1 | Sep 2022 | US |