Claims
- 1. A polynucleotide molecule comprising at least one gene of interest, and at least one selectable marker gene, wherein said at least one selectable marker gene comprises a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence encoding SEQ ID NOS.: 3, 4, or 5, or functional fragments thereof; or a complement of said nucleotide sequence; and (b) a nucleotide sequence which selectively hybridizes under stringent conditions to a nucleotide sequence shown in SEQ ID NOS: 1 or 2, or a complement thereof.
- 2. The polynucleotide molecule of claim 1, wherein said polynucleotide is operably linked to a promoter.
- 3. Transgenic cells transformed with a gene of interest and the polynucleotide molecule of claim 1, wherein the selectable marker gene gives said cells a selective advantage when a population of cells including the transformed cells and nontransformed cells is supplied with a marker compound.
- 4. The transgenic cells of claim 3 wherein said marker compound is arabitol, ribitol, mannitol or a derivative thereof.
- 5. The transgenic cells of claim 3, wherein said transgenic cells comprise bacteria, fungi, yeast, plant or a combination thereof.
- 6. A Plant or plant tissue regenerated from the cells of claim 3.
- 7. A method of selecting transformed cells from a population of cells comprising
a) introducing into the genome of a cell a gene of interest and a selectable marker gene; b) obtaining transformed cells; c) supplying to the population of cells a marker compound wherein said transformed cells have a selective advantage over non-transformed cells due to expression or transcription of the gene of interest or the selectable marker gene in the presence of the marker compound; and d) selecting said transformed cells from the population of cells; wherein said selectable marker gene comprises a nucleotide sequence selected from the group consisting of: (a) a nucleotide sequence encoding SEQ ID NOS.: 3, 4, or 5, or functional fragments thereof; or a complement of said nucleotide sequence; and (b) a nucleotide sequence which selectively hybridizes under stringent conditions to a nucleotide sequence shown in SEQ ID NOS: 1 or 2, or a complement thereof; and said marker compound comprises arabitol, ribitol, mannitol or a derivative thereof.
- 8. The method of claim 7, wherein said cells comprise bacteria, fungi, yeast, plant or a combination thereof.
- 9. The method of claim 8, wherein said cells comprise plant cells.
- 10. Transformed cells selected according to the method of claim 7.
- 11. Transformed plants derived from the cells of claim 10.
- 12. Seeds produced from the transformed plants of claim 11, wherein said seeds are capable of germinating to produce transformed plants.
- 13. A polynucleotide molecule comprising a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence encoding SEQ ID NOS.: 3, 4, or 5, or functional fragments thereof; or a complement of said nucleotide sequence; and (b) a nucleotide sequence which selectively hybridizes under stringent conditions to a nucleotide sequence shown in SEQ ID NOS: 1 or 2, or a complement thereof.
- 14. The polynucleotide molecule of claim 13, wherein said nucleotide sequence comprises SEQ ID NO 1.
- 15. The polynucleotide molecule of claim 13, wherein said nucleotide sequence comprises SEQ ID NO 2.
- 16. A polypeptide molecule comprising SEQ ID NO 3, or functional fragments thereof.
- 17. A polypeptide molecule comprising SEQ ID NO 4, or functional fragments thereof.
- 18. A polypeptide molecule comprising SEQ ID NO 5, or functional fragments thereof.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit under 35 USC §119(e) of Provisional Application No. 60/188,291 filed Mar. 8, 2000 and 60/225,595 filed Aug. 15, 2000.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60188291 |
Mar 2000 |
US |
|
60225595 |
Aug 2000 |
US |