Claims
- 1. A composition comprising a disposable arrayed prism configured for use in label free detection, said prism having an array of biological macromolecules thereon.
- 2. The composition of claim 1, wherein said label free detection comprises SPR.
- 3. The composition of claim 2, wherein said prism has an SPR capable metal film on one face.
- 4. The composition of claim 1, wherein said array of biological macromolecules comprises at least 50 distinct biological macromolecules.
- 5. The composition of claim 1, wherein said array of biological macromolecules comprises at least 100 distinct biological macromolecules.
- 6. The composition of claim 1, wherein said array of biological macromolecules comprises at least 1000 distinct biological macromolecules.
- 7. The composition of claim 3, wherein said SPR capable metal film is gold.
- 8. The composition of claim 1, wherein said disposable arrayed prism further comprises a plurality of microfluidics channels.
- 9. The composition of claim 8, wherein said microfluidics channels are one-dimensional line arrays.
- 10. The composition of claim 8, wherein said microfluidics channels are two-dimensional arrays.
- 11. The composition of claim 8, wherein said microfluidics channels are fabricated in poly(dimethylsiloxane).
- 12. The composition of claim 3, wherein said disposable arrayed prism further comprises a plurality of microchannels etched in said SPR capable metal film.
- 13. The composition of claim 1, wherein said biological macromolecule is selected from the group consisting of nucleic acids, proteins, carbohydrates, and amino acids.
- 14. A system, comprising a composition comprising a disposable arrayed prism, said prism having an array of biological macromolecules thereon; and a detection apparatus in communication with said disposable arrayed prism, and wherein said prism is configured for label free detection.
- 15. The system of claim 14, wherein said label free detection comprises SPR.
- 16. The system of claim 14, wherein said prism has an SPR capable metal film on one face.
- 17. The system of claim 14, wherein said array of biological macromolecules comprises at least 50 distinct biological macromolecules.
- 18. The system of claim 14, wherein said array of biological macromolecules comprises at least 100 distinct biological macromolecules.
- 19. The system of claim 14, wherein said array of biological macromolecules comprises at least 1000 distinct biological macromolecules.
- 20. The system of claim 16, wherein said SPR capable metal film is gold.
- 21. The system of claim 14, wherein said disposable arrayed prism further comprises a plurality of microfluidics channels.
- 22. The system of claim 21, wherein said microfluidics channels are one-dimensional line arrays.
- 23. The system of claim 21, wherein said microfluidics channels are two-dimensional arrays.
- 24. The system of claim 21, wherein said microfluidics channels are fabricated in poly(dimethylsiloxane).
- 25. The system of claim 16, wherein said disposable arrayed prism further comprises a plurality of microchannels etched in said SPR capable metal film.
- 26. The system of claim 14, wherein said biological macromolecule is selected from the group consisting of nucleic acids, proteins, carbohydrates, and amino acids.
- 27. The system of claim 14, wherein said detection apparatus is an SPR detection apparatus.
- 28. The system of claim 27, wherein said SPR apparatus further comprises a fluid handling device in communication with said microfluidics channels.
- 29. The system of claim 28, wherein said fluid handling device is configured to transfer fluids to said microfluidics channels.
- 30. A method of detecting interactions between biological molecules, comprising:
a) providing
i) a disposable arrayed prism having an array of target biological macromolecules thereon; ii) an apparatus configured for label-free detection; and iii) a sample comprising one or more biological molecules; and b) contacting said sample with said prism and said apparatus under conditions such that said apparatus detects interactions between said target biological molecules and said biological molecules.
- 31. The method of claim 30, wherein said disposable array prism is a disposable arrayed SPR prism, and wherein said prism has an SPR capable metal film on one face.
- 32. The method of claim 30, wherein said prism comprises at least 50 distinct target biological macromolecules.
- 33. The method of claim 30, wherein said prism comprises at least 100 distinct target biological macromolecules.
- 34. The method of claim 30, wherein said prism comprises at least 1000 distinct target biological macromolecules.
- 35. The method of claim 31, wherein said SPR capable metal film is gold.
- 36. The method of claim 31, wherein said disposable arrayed SPR prism further comprises a plurality of microfluidics channels.
- 37. The method of claim 36, wherein said microfluidics channels are one-dimensional line arrays.
- 38. The method of claim 36, wherein said microfluidics channels are two-dimensional arrays.
- 39. The method of claim 36, wherein said microfluidics channels are fabricated in poly(dimethylsiloxane).
- 40. The method of claim 31, wherein said disposable arrayed SPR prism further comprises a plurality of microchannels etched in said SPR capable metal film.
- 41. The method of claim 30, wherein said biological macromolecule is selected from the group consisting of nucleic acids, proteins, carbohydrates, and amino acids.
- 42. The method of claim 41, wherein said nucleic acids are selected from the group consisting of DNA and RNA.
- 43. The method of claim 30, wherein said label free detection apparatus is an SPR apparatus.
- 44. The method of claim 43, wherein said SPR apparatus further comprises a fluid handling device.
- 45. The method of claim 44, wherein said fluid handling device is configured to transfer fluids to said microfluidics channels.
Parent Case Info
[0001] This application claims priority to provisional patent application serial No. 60/378,586, filed May 8, 2002, which is herein incorporated by reference in its entirety.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60378586 |
May 2002 |
US |