Claims
- 1. A control for use in a gene expression analysis system comprising:
(a) a known amount of at least one DNA selected from the group consisting of
(i) SEQ ID Nos: 1-23; (ii) a degenerate variant of the sequence set forth in (i); and (iii) a complement of the sequence set forth in (i) and (ii); or (b) a known amount of at least one mRNA transcribed from the group consisting of
(i) SEQ ID NOs: 24-46; (ii) a degenerate variant of the sequence set forth in (i); and (iii) a complement of the sequence set forth in (i) and (ii).
- 2. A method of using a control as a negative control in a gene expression analysis system comprising:
adding a known amount of said control DNA of claim 1, to a gene expression analysis system as a control sample; subjecting the sample to hybridization conditions in the absence of complementary labeled mRNA; examining the control sample for the absence or presence of signal.
- 3. A method of using controls in a gene expression analysis system comprising:
adding a known amount of said control DNA of claim 1, to a gene expression analysis system as a control sample; subjecting the sample to hybridization conditions in the presence of a known amount of labeled complementary mRNA of claim 1;measuring the signal values for the labeled mRNA and determining the expression level of the DNA based on the measured signal value.
- 4. A method of using controls as calibrators in a gene expression analysis system comprising:
adding a known amount of a said control containing known amounts of several DNAs of claim 1, to a gene expression analysis system as control samples; subjecting the samples to hybridization conditions in the presence of a said control containing known amounts of corresponding complementary labeled mRNAs of claim 1, each mRNA being at a different concentration; measuring the signal values for the labeled mRNAs and constructing a dose-response or calibration curve based on the relationship between signal value and concentration of each mRNA.
- 5. A method of using controls as calibrators for gene expression ratios in a two-color gene expression analysis system comprising:
adding a known amount of at least one of said controls containing a known amount of DNA of claim 1, to a two-color gene expression analysis system as control samples; subjecting the samples to hybridization conditions in the presence of a said control containing known amounts of two differently labeled corresponding complementary labeled mRNAs of claim 1, for each DNA sample present; measuring the ratio of the signal values for the two differently labeled mRNAs and comparing the signal ratio to the ratio of concentrations of the two or more differently labeled mRNAs.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part of U.S. patent application Ser. No. 10/140,545, filed May 7, 2002, which claims priority to U.S. provisional patent application Nos. 60/289,202, filed May 7, 2001, and 60/312,420, filed Aug. 15, 2001. This application also claims priority to U.S. provisional patent application Ser. Nos. 60/335,115, filed Oct. 24, 2001, and 60/391,367, filed Jun. 25, 2002, the disclosures of which are incorporated herein by reference in their entireties.
Provisional Applications (4)
|
Number |
Date |
Country |
|
60289202 |
May 2001 |
US |
|
60312420 |
Aug 2001 |
US |
|
60335115 |
Oct 2001 |
US |
|
60391367 |
Jun 2002 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
10140545 |
May 2002 |
US |
Child |
10278845 |
Oct 2002 |
US |