Claims
- 1. An artificial test soil comprising: base medium, serum, blood and endotoxin.
- 2. An artificial test soil according to claim 1 comprising up to 20% v/v serum; up to 10% v/v blood and up to 2,000,000 EU/ml endotoxin.
- 3. An artificial test soil according to claim 1 wherein the base medium is Roswell Park Memorial Institute medium (RPMI).
- 4. An artificial test soil according to claim 1 wherein the serum is calf serum.
- 5. An artificial test soil according to claim 1 wherein the endotoxin is derived from lipopolysaccharide (LPS).
- 6. An artificial test soil according to claim 1 further comprising bilirubin.
- 7. An artificial test soil according to claim 6 wherein the bilirubin is present in an amount of about 1,000 nmoles/ml.
- 8. An artificial test soil according to claim 7 where the bilirubin is derived from oxgall bile.
- 9. An artificial test soil according to claim 1 further comprising mucin or carbohydrate.
- 10. An artificial test soil according to claim 9 wherein the mucin or carbohydrate is present in the amount of about 10,000 μg/ml.
- 11. An artificial test soil according to claim 10 wherein the carbohydrate is derived from L-Glutamine.
- 12. An artificial test soil according to claim 1 that mimics a gastrointestinal site comprising:RPMI 1640 LPS Calf Serum Bovine Oxgall Sterile Sheep Blood Sodium Bicarbonate Sodium-Pyvuvate and L-Glutamine.
- 13. An artificial test soil according to claim 1 that mimics soil related to a lung site comprising:RPMI 1640 LPS Calf Serum Sterile Sheep Blood Sodium Bicarbonate Sodium-Pyvuvate and L-Glutamine.
- 14. A test kit comprising an artificial test soil according to claim 1 and an indicator that determines the presence of a contaminant.
- 15. A test kit according to claim 14 wherein an indicator can detect the presence of more than one contaminant.
- 16. A kit according to claim 15 wherein the indicator can detect the presence of blood, protein and leukocyte esterase.
- 17. A method of evaluating a reprocessing method on a device comprising:(a) applying an artificial test soil (ATS) comprising base medium, serum, blood and endotoxin to the device; (b) subjecting the device to the reprocessing method to be evaluated; and (c) determining the presence or absence of at least one contaminant on the device.
- 18. A method according to claim 17 wherein the contaminant is selected from the group consisting of blood, protein and leukocyte esterase.
- 19. A method according to claim 17 wherein the device is a medical device.
- 20. A method according to claim 19 wherein the medical device is selected from the group consisting of flexible endoscopes, narrowed lumened accessory devices and balloon catheters.
- 21. A method of determining whether a reprocessing method can kill a microorganism on a device comprising:(a) inoculating an artificial test soil (ATS) with a microorganism wherein the ATS comprises base medium, serum, blood or hemoglobin and endotoxin; (b) applying the inoculated ATS to the device; (c) subjecting the device to the reprocessing method to be evaluated; and (d) determining the presence or absence of the microorganism on the device, wherein the absence of the microorganism on the device indicates that reprocessing method can kill the microorganism.
Parent Case Info
This application is a national phase entry under 35 USC 371 of PCT/CA99/00727 filed Aug. 9, 1999 which claims priority from U.S. provisional application serial No. 60/095,932. filed Aug. 10, 1998 (now abandoned), which is incorporated herein by reference.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
PCT/CA99/00727 |
|
WO |
00 |
Publishing Document |
Publishing Date |
Country |
Kind |
WO00/09743 |
2/24/2000 |
WO |
A |
Foreign Referenced Citations (4)
Number |
Date |
Country |
0 884 115 |
Dec 1998 |
EP |
0 884 115 |
Dec 1998 |
EP |
WO 9727482 |
Jul 1997 |
WO |
WO 9840736 |
Sep 1998 |
WO |
Provisional Applications (1)
|
Number |
Date |
Country |
|
60/095932 |
Aug 1998 |
US |