Aryl carbamate derivatives, preperation and use thereof

Abstract

The present invention relates to novel compounds, the preparation and use, particularly therapeutic, thereof. More specifically, it relates to compounds derived from aryl carbamates, the preparation and use thereof, particularly in the field of human and animal health. The compounds according to the invention are preferably 5-HT4 serotoninergic receptor ligands and can therefore be used in the therapeutic or prophylactic treatment of any disorder involving a 5-HT4 receptor. The invention also relates to pharmaceutical compositions comprising such compounds, the preparation and use thereof and treatment methods using said compounds.

Description

[0001] The present invention relates to novel compounds, the preparation and use, particularly therapeutic, thereof. More specifically, it relates to compounds derived from aryl carbamates, the preparation and use thereof, particularly in the field of human and animal health. The compounds according to the invention are preferably 5-HT4 serotoninergic receptor ligands and can therefore be used in the therapeutic or prophylactic treatment of any disorder involving a 5-HT4 receptor. The invention also relates to pharmaceutical compositions comprising such compounds, the preparation and use thereof and treatment methods using said compounds.

[0002] A large number of serotonin-dependent processes have been identified to date, and many molecules acting at serotonin receptors are used in human therapeutics. More than a dozen serotonin receptors have been identified, one of the most recent being the 5-HT4 receptor (J. Bockaert et al., Trends Pharmacol. Sci., 13, 141, 1992). The present invention relates to aryl carbamate derivatives represented by the general formula (I) and their pharmacologically acceptable salts. They are preferably compounds capable of interfering with serotonin-dependent processes, even more preferably 5-HT4 receptor ligands, particularly the human serotype thereof. The invention thus discloses methods of treatment or prophylaxis of any disorder involving a 5-HT4 receptor. The compounds and compositions according to the invention may be proved useful in the therapeutic or prophylactic treatment of different pathologies such as

[0003] various gastrointestinal disorders such as gastroesophageal reflux disease (GERD), irritable bowel syndrome, functional dyspepsia, gastroparesis, disorders linked to intestinal motility, nausea, and constipation,

[0004] central nervous system disorders such as anxiety, pain, schizophrenia, depression, memory impairment and dementia,

[0005] cardiac disorders such as atrial fibrillation, arrhythmia and tachycardia,

[0006] urologic disorders such as urinary retention, incontinence.

[0007] A first object of the invention is based on compounds represented by the general formula (I): 1

[0008] wherein:

[0009] R1 represents a lower alkyl or a lower arylalkyl group,

[0010] R2 represents a hydrogen atom or a lower alkyl group,

[0011] R3 represents an aryl group or a heterocycle, chosen from among phenyl, naphthyl, thienyl, furyl, pyridyl, pyrimidinyl, pyridazinyl, pirazinyl, such group possibly being substituted by one or more groups chosen from among halogeno, alkoxy, cyano, hydroxy, nitro, alkyl, halogenoalkyl, alkylthio, —NHR8, —NHCOR8, —NHSO2R8, —NHCONR8R9

[0012] Y represents a linear or branched alkylene chain or bond, containing 2 to 5 carbon atoms,

[0013] J represents a C—R7 group or the nitrogen atom,

[0014] G represents a C—R5 group or the nitrogen atom,

[0015] R4, R5, R6 and R7 taken individually represent the hydrogen atom, a halogen atom, a lower alkyl group, an alkoxy, alkylthio, alkylsulfonyl, alkylsulfoxide, trifluoromethyl, nitro, cyano, carboxy, carboxyalkyl, alkylamino or dialkylamino group,

[0016] R8 and R9 represent the hydrogen atom, an aryl group or a lower alkyl group,

[0017] or, when G or J are not the nitrogen atom, the groups OR, and R7 and/or the groups R6 and R7 and/or the groups R6 and R5 and/or the groups R5 and R4 may form, together with the aromatic ring to which they are attached, a saturated or unsaturated ring,

[0018] n is equal to 1 or 2,

[0019] said alkyl, alkylene, aryl, arylalkyl, heterocycle and carboxyalkyl groups, and the ring, being substituted or not,

[0020] with the exception of compounds represented by formula (I) wherein:

[0021] Y represents a 1-(methoxymethyl)ethyl or 2-hydroxypropyl group, R3 represents a phenyl or 2-methoxyphenyl group, when R1 represents a lower alkyl group, R2, R4 and R6 represent a hydrogen atom, G and J represent a CH group and n is equal to 1,

[0022] and the salts thereof.

[0023] According to the present invention, the term “lower alkyl” more specifically denotes a linear or branched hydrocarbon group having from 1 to 6 carbon atoms such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl, neopentyl, n-hexyl. C1-C4 groups are preferred. Methyl and ethyl groups are especially preferred.

[0024] “Aryl” groups are mono-, bi- or tri-cyclic aromatic hydrocarbon systems having from 6 to 18 carbon atoms. The phenyl and naphthyl groups are specific examples.

[0025] “Alkylene” groups in the context of the invention are divalent groups corresponding to the alkyl groups defined hereinabove in which a hydrogen atom has been removed.

[0026] “Alkoxy” groups correspond to the alkyl groups defined hereinabove bonded to the ring by means of an —O— (ether) bond.

[0027] “Alkylthio” groups correspond to the alkyl groups defined hereinabove bonded to the ring by means of an —S— (thioether) bond.

[0028] “Halogen” denotes a fluorine, chlorine, bromine or iodine atom.

[0029] “Halogenoalkyl” denotes an alkyl group such as defined hereinabove in which one or more hydrogen atoms have been replaced by one or more halogen atoms. The trifluoromethyl function is a specific example.

[0030] “Heteroatom” denotes an atom chosen from among N, O and S.

[0031] Arylalkyl groups are groups comprising an aryl function as defined hereinabove bonded to the ring by means of an alkylene chain.

[0032] When the OR1 and R7 groups and/or the R6 and R7 groups and/or the R6 and R5 groups and/or the R5 and R4 form, together with the aromatic ring to which they are attached, a saturated or unsaturated ring, it is preferably a ring comprising from 3 to 8 atoms, aromatic or not, possibly containing one or more heteroatoms, preferably 0 to 3. Preferred examples of such rings include in particular benzofuran, dihydrobenzofuran, benzodioxane, benzopyran, dihydrobenzopyran, benzodioxole.

[0033] Furthermore, as indicated hereinabove, these different groups may or may not contain one or more substituents, chosen for example from among halogen, nitro, cyano, trifluoromethyl, carboxy, (C1-C6)-alkoxycarbonyl, mono- or di-(C1-C6)— alkylaminocarbonyl, aminocarbonyl, mono- or di-(C6-C12)-aryl- or hetero-(C2-C12)— arylaminocarbonyl, mono- or di-(C6-C12)-aryl- or hetero-(C2-C12)-aryl-(C1-C6)— alkylaminocarbonyl, hydroxy, alkoxy, (C1-C6)-alkyl, amino possibly substituted by one or more groups chosen from among (C1-C6)-alkyl, (C3-C8)-cycloalkyl, (C2-C12)-aryl, hetero-(C2-C12)-aryl, (C6-C12)-aryl-(C1-C6)-alkyl, hetero-(C2-C12)-aryl-(C1-C6)-alkyl, (C1-C7)— alcanoyl, cyclo-(C3-C8)-alcanoyl, (C6-C12)-aroyl, or (C6-C12)-aryl-(C1-C7)-alcanoyl.

[0034] The compounds of the present invention display excellent selectivity for the 5-HT4 receptor as compared to other 5-HT receptor subtypes and particularly as compared to the 5-HT3 receptor. For instance, the compound 2-[4-(4-pyridinyl)piperazino]ethyl-N-(2-methoxyphenyl) carbamate (63) has a Ki of 5 tiM for the 5-HT4 receptor and no activity up to a concentration of 1 μM for the 5-HT1A, 5-HT1B, 5-HT2A, 5-HT2C, 5-HT3, 5-HT5A, 5-HT6 or 5-HT7 receptors.

[0035] Furthermore, in an advantageous manner, the compounds of the present invention, in contrast to other known 5-HT4 or 5-HT3 ligands like Cisapride, do not have side effects such as serious cardiovascular complications.

[0036] Preferred compounds of the invention are compounds represented by formula (I) hereinabove wherein:

[0037] R1 represents a methyl or ethyl group, and/or

[0038] R3 represents an aryl group or a heterocycle having 6 atoms, possibly substituted, preferably containing one or two nitrogen atoms, or a phenyl group possibly substituted, and/or

[0039] n=1 and/or

[0040] Y is an alkylene chain of 2 or 3 carbon atoms, preferably linear, and/or

[0041] R2 is a hydrogen atom, and/or

[0042] R4 is a hydrogen atom, and/or

[0043] R6 is a hydrogen atom, and/or

[0044] G is a CH group, and/or

[0045] J is aCH group.

[0046] A preferred subfamily according to the invention is represented by compounds having formula (I) wherein R1 is a methyl or ethyl group. As illustrated in the examples, such derivatives according to the invention display advantageous properties as 5-HT4 receptor ligands.

[0047] An especially preferred family is that in which R1 represents a methyl or ethyl group. Another particular category of compounds according to the invention is represented by compounds having the general formula (I) wherein R3 represents a heterocycle of 6 atoms, possibly substituted, containing one or two nitrogen atoms or a phenyl group, possibly substituted.

[0048] According to an especially preferred variant, the invention has as its object compounds represented by formula (I) wherein R1 is a methyl or ethyl group and R3 represents a phenyl group possibly substituted.

[0049] According to another especially preferred variant, the invention has as its object compounds represented by formula (I) wherein R1 is a methyl or ethyl group and R3 is a heterocycle of 6 atoms, possibly substituted, containing one or two nitrogen atoms.

[0050] A specific family according to the invention comprises compounds represented by general formula (I) such as defined hereinabove, and the subfamilies indicated hereinabove, wherein R2 is a hydrogen atom, and/or R4 is a hydrogen atom, and/or R6 is a hydrogen atom, wherein even more preferably at least two of the groups R2, R4 and R6 are a hydrogen atom, even more preferably wherein the three groups R2, R4 and R6 each represent a hydrogen atom.

[0051] Another specific family according to the invention comprises compounds represented by general formula (I) such as defined hereinabove, and the subfamilies indicated hereinabove, wherein G is the CH group and/or J is the CH group, more preferably wherein G and J each represent a CH group.

[0052] A further specific family according to the invention comprises compounds represented by general formula (I) such as defined hereinabove, and the subfamilies indicated hereinabove, wherein n is equal to 1.

[0053] Another specific family according to the invention comprises compounds represented by general formula (I) such as defined hereinabove, and the subfamilies indicated hereinabove, wherein Y is an alkylene chain containing 2 or 3 carbon atoms, preferably not branched.

[0054] As noted, the compounds of the invention may be in the form of salts, particularly acid or base salts, preferably compatible with pharmaceutical use.

[0055] Non-limiting examples of pharmaceutically acceptable acids include hydrochloric, hydrobromic, sulfuric, phosphoric, acetic, trifluoroacetic, lactic, pyruvic, malonic, succinic, glutaric, fumaric, tartaric, maleic, citric, ascorbic, methane or ethanesulfonic, camphoric acids, etc. Non-limiting examples of pharmaceutically acceptable bases include sodium hydroxide, potassium hydroxide, triethylamine and tert-butylamine.

[0056] Specific examples of preferred compounds according to the invention are the following compounds in particular:

[0057] 2-[4-(3-methoxyphenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 15

[0058] 2-[4-(3-methylphenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 21

[0059] 2-[4-(2-pyridinyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 24

[0060] 2-[4-(3-pyridazinyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 25

[0061] 3-[4-(4-fluorophenyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 38

[0062] 3-[4-(2-pyridinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 40

[0063] 3-[4-(4-pyridinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 41

[0064] 3-[4-(3-pyridazinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 42

[0065] 3-[4-(4-pyrimidinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 43

[0066] 3-[4-(2-pyrimidinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 44

[0067] 3-[4-(6-methyl-3-pyridazinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 48

[0068] 2-(4-phenylpiperazino)ethyl-N-(2-methoxyphenyl)carbamate 49

[0069] 2-[4-(3-methoxyphenyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 51

[0070] 2-[4-(2-pyridinyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 62

[0071] 2-[4-(3-pyridazinyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 64

[0072] 2-[4-(2-pyrimidinyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 65

[0073] 3-[4-(4-pyridinyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 83

[0074] such as described in the examples, and the salts thereof, particularly compounds 15, 24, 25, 38,40,41,49.

[0075] Compounds represented by formula (I) may be prepared by methods known to those skilled in the art. In this regard, the present invention describes different synthetic routes, illustrated in FIGS. 1-5 and in the examples, and which may be utilized by those skilled in the art, as indicated in the examples. The starting compounds may be obtained commercially or synthesized by the usual methods. It is understood that the present application is not restricted to a particular route of synthesis, and encompasses other methods by which to produce the indicated compounds. As an example, the compounds represented by formula (I) may be synthesized either in liquid phase as depicted in illustrations 1-4 (FIGS. 1-4) or by parallel synthesis on a solid support according to illustration 5 (FIG. 5).

[0076] According to a particular object, the invention is based on a method for preparing a compound such as defined hereinabove, wherein a product represented by formula (II) is reacted with a product represented by formula (III): 2

[0077] in which the groups R1, R2, R3, R4, R6, Y, J, G and n are defined as hereinabove, in the presence of a carbonyl donor reagent, preferably the (Boc)2O/DMAP system, and the resulting product is recovered. The reaction is advantageously carried out in a solvent, for example neutral, typically an aprotic solvent (see FIG. 1).

[0078] Another particular object of the invention is a method for preparing a compound such as defined hereinabove, wherein a product represented by formula (II) is reacted with a product represented by formula (III) in which the groups R1, R2, R3, R4, R6, Y, J, G and n are defined as hereinabove, in a CXCl2/pyridine system, and the resulting product is recovered. The reaction is advantageously carried out in a solvent, for example neutral, typically an aprotic solvent (see FIG. 2).

[0079] A further specific object of the invention is a method for preparing a compound such as defined hereinabove, wherein a product represented by formula (II) is reacted with a product represented by formula (XVI) and a product represented by formula (XVII), in which the groups R1, R2, R3, R4, R6, Y, J, G and n are defined as hereinabove, and the resulting product is recovered. The reaction is advantageously carried out in the presence of Et2O (see FIG. 3).

[0080] An additional specific object of the invention is a method for preparing a compound such as defined hereinabove, wherein a product represented by formula (II) is reacted with a product represented by formula (XVIII) in which the groups R1, R2, R3, R4, R6, Y, J, G and n are defined as hereinabove, and the resulting product is recovered. The reaction is advantageously carried out in a solvent, for example CH3CN or dioxane (see FIG. 4).

[0081] According to another variant, the invention relates to a method for preparing a compound such as described hereinabove, wherein a product represented by formula (XIV) is reacted with a product represented by formula (XII): 3

[0082] in which the groups R1, R2, R3, R4, Y, J, G and n are defined as hereinabove and K is a spacer group, in the presence of DIEA, and wherein the resulting product is released by chemical cleavage.

[0083] More specifically, the spacer group K has the following formula (XV): 4

[0084] the product then having the structure of the compound represented by formula (XI).

[0085] Moreover, the method advantageously comprises preparing the compound represented by formula (XIV) [or formula (XI)] according to the reaction route shown in FIG. 5.

[0086] Another object of the present invention relates to any pharmaceutical composition comprising a compound such as defined hereinabove. Such pharmaceutical composition is advantageously for the treatment or prophylaxis of diseases involving a 5-HT4 receptor, for example the 5-HT4e receptor. The pharmaceutical compositions according to the invention may be used in particular for the treatment or prophylaxis of gastrointestinal disorders, central nervous system disorders, cardiac disorders or urologic disorders.

[0087] The invention equally relates to the use of a compound such as defined hereinabove for preparing a pharmaceutical composition for implementing a method of treatment or prophylaxis in the human or animal body.

[0088] The invention further concerns a method for treating a disease involving a 5-HT4 receptor, comprising administering to a subject, particularly human, an effective dose of a compound or a pharmaceutical composition such as defined hereinabove.

[0089] The pharmaceutical compositions of the invention advantageously contain one or more pharmaceutically acceptable excipients or vehicles. These may be exemplified by saline, physiological, isotonic, buffered solutions, etc., compatible with pharmaceutical use and known to those skilled in the art. The compositions may contain one or more agents or vehicles chosen from among dispersives, solubilizers, stabilizers, preservatives, and the like. Agents or vehicles that may be used in the formulations (liquids and/or injectables and/or solids) comprise in particular methylcellulose, hydroxymethylcellulose, carboxymethylcellulose, polysorbate 80, mannitol, gelatine, lactose, vegetable oil, acacia, and the like. The compositions may be formulated as a suspension for injection, gels, oils, tablets, suppositories, powders, capsules, gelules, and the like, possibly by means of pharmaceutical forms or devices allowing sustained and/or delayed release. For this type of formulation, an agent such as cellulose, carbonates or starches is advantageously used.

[0090] The compounds or compositions according to the invention may be administered in various ways and in different forms. For instance, they may be injected by the systemic route or given orally, preferably by the systemic route, such as for example by the intravenous, intramuscular, subcutaneous, transdermal, intra-arterial route, etc. For injections, the compounds are generally prepared as liquid suspensions, which may be injected through syringes or by infusion, for instance. It is understood that the rate and/or injected dose may be adapted by those skilled in the art according to the patient, the pathology, the method of administration, etc. Typically, the compounds are administered at doses ranging from 0.1 μg to 100 mg/kg of body weight, more generally from 0.01 to 10 mg/kg, typically between 0.1 and 10 mg/kg. Furthermore, repeated injections may be given, as the case may be. Moreover, the compositions according to the invention may additionally comprise other active substances or agents.

[0091] Other aspects and advantages of the present invention will become apparent in the following examples, which are given for purposes of illustration and not by way of limitation.

LEGENDS OF FIGURES

[0092] FIG. 1: Route of synthesis 1 of compounds according to the invention. Y, G, J and groups R1-R9 are defined as hereinabove.

[0093] FIG. 2: Route of synthesis 2 of compounds according to the invention. Y, G, J and groups R1-R9 are defined as hereinabove.

[0094] FIG. 3: Route of synthesis 3 of compounds according to the invention. Y, G, J and groups R1-R9 are defined as hereinabove.

[0095] FIG. 4: Route of synthesis 4 of compounds according to the invention. Y, G, J and groups R1-R9 are defined as hereinabove.

[0096] FIG. 5: Route of synthesis 5 of compounds according to the invention. Y, G, J and groups R1-R9 are defined as hereinabove.

MATERIALS AND METHODS

[0097] The compounds of the invention were obtained through classic methods of parallel synthesis and organic synthesis.

[0098] The 1H and 13C NMR spectra were recoded on a Brucker AC-200 spectrometer. Chemical shifts are given in ppm with tetramethylsilane as internal reference. The symbols m, s, sl, d, t, q, quint., dd, td, etc. respectively denote multiplet, singlet, wide singlet, doublet, triplet, quadruplet, quintuplet, split doublet, split triplet. Infrared spectra were recorded on a Perkin Elmer 841 instrument as KBR disks or on a Brucker vector 22 Fourier transform instrument.

[0099] Melting points were determined in a Kofler apparatus. HPLC spectra were recorded on a Shimadzu SCL10A with a Uptisphere UP50 DB-5m C18 column (4.6×50 mm) at a flow rate of 4 ml/min and a wavelength of 220 nm.

[0100] HPLC/MS analyses were carried out on a LC Micromass Plateform spectrometer (TSK gel super ODS column, 4.6 mm ID×5 cm, flow rate 2.75 ml/min, gradient: 100% A to 100% B in 3 min, 1 min plateau at 100% B; solvent A=water/0.05% trifluoroacetic acid, solvent B=acetonitrile/water/trifluoroacetic acid (80:20:0.05).

[0101] Unless otherwise indicated, the products used to prepare compounds represented by formula (I) were obtained commercially and used without out further purification. The experimental protocols described below are given for purposes of illustration and not at all by way of limitation. Unless otherwise indicated, percentages are expressed on a weight basis.

EXAMPLE 1

4-bromo-2-methoxyaniline 2

[0102] At room temperature, 3.4 ml (30 mmol, 1.25 eq) of 2-methoxyaniline is diluted in 10 ml of acetic acid. Over approximately 1 hour, 1.2 ml (24 mmol, 0.8 eq) of bromine dissolved in 10 ml of acetic acid is added dropwise. A purple precipitate is formed. When all the bromine has been added, the hydrobromide is filtered on a Buchner funnel and rinsed with acetic acid. Take up the solid in a H2O/CH2Cl2 mixture. A slight excess of KOH pellets is added, the aqueous phase is extracted with CH2Cl2 and the organic phase is dried on MgSO4. Concentration under reduced pressure gives a brown oil which is purified by column chromatography (eluent CH2Cl2). The expected product (3.46 g) is a colorless oil (yield: 57%). 5

[0103] C7H8BrNO

[0104] M=202.06 g/mol

[0105] 1 H NMR (CDCl3) δ (ppm): 6.89 (dd, Jo=8.7 Hz, Jm=2.03 Hz, 1H, CH(s)); 6.87 (sl, 1H, CH(3)); 6.57 (dd, Jo=8.7 Hz, Jp=1.96 Hz, 1H, CH(6)); 3.83 (s, 3H, OCH3); 3.76 (sl, 2H, NH2).

[0106] 13 C NMR (CDCl3) δ (ppm): 147.9 (C2); 135.6 (C1); 123.7 (C5); 115.8 (C6); 113.7 (C3); 109.4 (C4); 55.7 (OCH3).

Elemental analysis:
		Calc.	Exp.
	% C	41.61	41.48
	% H	3.99	3.90
	% N	6.93	6.85

EXAMPLE 2

tert-butyl-N-(4-bromo-2-methoxyphenyl)carbamate 4

[0107] In a 250 ml single-necked flask placed under an inert atmosphere, 6.4 g (31.7 mmol) of 4-bromo-2-methoxyaniline 2 and 7.6 g (34.9 mmol, 1.1 eq) of (Boc)2O are dissolved in 80 ml of anhydrous THF. Heat the THF under a reflux condenser for 15 hours. The medium is allowed to cool and the solvent is evaporated. The residue is taken up in CH2Cl2, washed with a saturated aqueous solution of NaHCO3 (3×). The organic phase is dried on MgSO4. A concentration under vacuum is implemented to produce a brown oil which is purified by column chromatography (eluent petroleum ether/AcOEt 9:1). 9.6 g of 4 is obtained as a colorless oil (yield: 100%). 6

[0108] C12H16BrNO3

[0109] M=302.19 g/mol

[0110] 1 H NMR (CDCl3) δ (ppm): 7.95 (d, J=8.6 Hz, 1H, CH(6)); 7.07-6.94 (m, 3H, NH, CH(3), CH(5)); 3.84 (s, 3H, OCH3); 1.52 (s, 9H, CH3).

[0111] 13 C NMR (CDCl3) δ (ppm): 152.5 (CO2); 148.1 (C2); 127.4 (CI); 123.8 (C5); 119.1 (C6); 114.3 (C4); 113.4 (C3); 80.5 (C(CH3)3); 55.9 (OCH3); 28.3 (3C, CH3).

EXAMPLE 3

tert-butyl-N-{4-[allyl(dimethyl)silyl]-2-methoxyphenyl}carbamate 6

[0112] To 2.4 g (20.6 mmol, 1.2 eq) of 35% potassium hydride suspended in 70 ml of anhydrous THF under an argon atmosphere, at room temperature 5.2 g (17.2 mmol) of tert-butyl-N-(4-bromo-2-methoxyphenyl)carbamate 4 diluted in 60 ml of anhydrous THF is added dropwise. This mixture is shaken for 10 min before cooling to −78° C. When this temperature is reached, 23.5 ml (35.3 mmol, 2.05 eq) of 1.5 M tert-butyllithium is added through a cannula in hexane diluted in 50 ml of anhydrous THF and previously cooled to −78° C. The reaction is allowed to progress for 20 min at −78° C. before adding 6.2 ml (41.3 mmol, 2.4 eq) of allyl(chloro)dimethylsilane all at once. The temperature is allowed to rise to −10° C. then the medium is hydrolyzed with a small amount of water. Next, 90 ml of AcOEt is added and the organic phase is washed with H2O (3×90 ml), 1 M NaHSO4 (2×90 ml), then a saturated aqueous solution of NaCl (1×90 ml), and lastly, dried on MgSO4. Concentration under vacuum gives an orange oil which is purified by column chromatography (eluent petroleum ether/AcOEt 9:1), producing 4.28 g of a colorless oil (yield: 77%). 7

[0113] C17H27NO3Si

[0114] M=321.47 g/mol

[0115] 1 H NMR (CDCl3) δ (ppm): 8.05 (d, J=8 Hz, 1H, CH(6)); 7.12 (sl, 1H, NH); 7.08 (dd, J=8 Hz, J=1.2 Hz, 1H, CH(5)); 6.94 (d, J=1.2 Hz, 1H, CH(3)); 5.78 (ddt, J=16.9 Hz, J=10.1 Hz, J=8.1 Hz, 1H, CH(2′)); 4.86 (m, 2H, CH2(1′)); 3.88 (s, 3H, OCH3); 1.74 (dt, J=8.1 Hz, J=1.2 Hz, 2H, CH2Si); 1.52 (s, 9H, CH3); 0.26 (s, 6H, (CH3)2Si).

[0116] 13 C NMR (CDCl3) δ (ppm): 152.6 (CO2); 146.9 (C2); 134.7 (C2′); 131.7 (C4); 128.9 (C1); 126.7 (C5); 117.4 (C6); 114.3 (C3); 113.2 (C1′); 80.3 (C(CH3)3); 55.5 (OCH3); 28.8 (3C, CH3); 23.8 (C3′); −3.4 (2C, CH3Si).

EXAMPLE 4

4-bromo-2-ethoxyaniline 3

[0117] Over approximately 1 hour, 3 ml (58.4 mmol) of bromine dissolved in 20 ml of acetic acid is added dropwise to 7.8 ml (60 mmol, 1.03 eq) of 2-ethoxyaniline diluted in 20 ml of acetic acid. When all the bromine has been added, the precipitate formed is filtered on a Buchner funnel and rinsed with acetic acid. The hydrobromide is taken up in H2O, 5 g (90 mmol, 1.5 eq) of KOH pellets is added and extracted with CH2Cl2. The organic phase is dried on MgSO4 and the solvent is evaporated. The residue is purified by column chromatography (eluent CH2Cl2/pentane 9:1) to produce 7.52 g of the expected product as a brown oil (yield: 58%). 8

[0118] C8H10BrNO

[0119] M=216.09 g/mol

[0120] 1 H NMR (CDCl3) δ (ppm): 6.89 (dd, Jo=8.6 Hz, Jm=2 Hz, 1H, CH(5)); 6.87 (sl, 1H, CH(3)); 6.57 (dd, Jo=8.6 Hz, Jp=2 Hz, 1H, CH(6)); 4.02 (q, J=7 Hz, 2H, CH2O); 3.56 (sl, 2H, NH2); 1.43 (t, J=7 Hz, 3H, CH3).

EXAMPLE 5

tert-butyl(N-(4-bromo-2-ethoxyphenyl)carbamate 5

[0121] The reaction between 7.2 g (33.3 mmol) of 4-bromo-2-ethoxyaniline 3 and 8 g (36.6 mmol, 1 eq) of (Boc)2O in 90 ml of anhydrous THF under the conditions described for the synthesis of 4, followed by purification by column chromatography (eluent petroleum ether/AcOEt 9:1), produced 10.5 g of the expected product in the form of white crystals following crystallization of the resulting light yellow oil in pentane (yield: 100%). 9

[0122] M=316.98 g/mol

[0123] F=72° C.

[0124] 1 H NMR (CDC3) δ (ppm): 7.96 (d, J=8.6 Hz, 1H, CH(6)); 7.03 (dd, J=8.6 Hz, J=2 Hz, 1H, CH(5)); 6.98 (sl, 1H, NH); 6.92 (d, J=2 Hz, 1H, CH(3)); 4.06 (q, J=7 Hz, 2H, CH2O); 1.52 (s, 9H, CH3); 1.45 (t, J=7 Hz, CH3).

[0125] 13 C NMR (CDCl3) δ (ppm): 152.5 (CO2); 147.4 (C2); 127.4 (C1); 123.7 (C5); 119.1 (C6); 114.4 (C4); 113.3 (C3); 80.6 (C(CH3)3); 64.5 (CH2O); 28.3 (3C, CH3); 14.7 (CH3).

EXAMPLE 6

tert-butyl-N-{4-[allyl(dimethyl)silyl]-2-ethoxyphenyl}carbamate 7

[0126] The reaction between 5.3 g (16.8 mmol) of tert-butyl-N-(4-bromo-2-ethoxyphenyl)carbamate 5, 2.4 g (20.16 mmol, 1.2 eq) of 35% potassium hydride, 20.2 ml (34.4 mmol, 2.05 eq) of 1.5 M tert-butyllithium in hexane and 6 ml (40.3 mmol, 2.4 eq) of allyl(chloro)dimethylsilane according to the procedure described for compound 6, followed by two successive purifications by column chromatography (eluant petroleum ether/AcOEt 9:1), lead to 3.3 g of a colorless oil (yield: 58%). 10

[0127] C17H27NO3Si

[0128] M=335.56 g/mol

[0129] 1 H NMR (CDCl3) δ (ppm): 8.02 (d, J=8 Hz, 1H, CH(6)); 7.10 (sl, 1H, NH); 7.06 (d, J=8 Hz, 1H, CH(5)); 6.93 (s, 1H, CH(3)); 5.78 (ddt, J=16.6 Hz, J=10.2 Hz, J=8.1 Hz, 1H, CH(2′)); 4.89-4.83 (m, 2H, CH2(1′)); 4.12 (q, J=7 Hz, 3H, CH2O); 1.74 (d, J=8.1 Hz, 2H, CH2(3′)); 1.54 (s, 9H, CH3); 1.46 (t, J=7 Hz, 3H, CH3); 0.25 (s, 6H, (CH3)2Si).

[0130] 13 C NMR (CDCl3) δ (ppm): 152.6 (CO2); 146.2 (C2); 134.8 (C2′); 131.5 (C4); 129 (C1); 127 (C5); 118 (C6); 115.5 (C3); 113 (C1); 80 (C(CH3)3); 65 (CH2O); 28.5 (3C, CH3); 24 (C3′); 15 (CH3); −3.4 (2C, CH3Si).

EXAMPLE 7

tert-butyl-N-(2-methoxyphenyl)carbamate Bonded to a Solid Support 8

[0131] In a 500 ml three-necked flask under an argon atmosphere, dissolve 6 g (18.7 mmol, 1.7 eq) of tert-butyl-N-{4-[allyl(dimethyl)silyl]-2-methoxyphenyl}carbamate 6 in 84 ml of anhydrous THF. Next, add 33.6 ml (16.8 mmol, 1.5 eq) of 0.5 M 9-BBN in anhydrous THF. The reaction is allowed to progress at room temperature and under argon for 5 hours. Then 0.4 g (0.33 mmol, 3% eq) of Pd(PPh3)4, 9 ml (1.7 eq) of an aqueous 2 M Na2CO3 solution, and 2.8 g (11 mmol) of bromopolystyrene resin (substituted 4 mmol/g) are added. The flask is wrapped in a sheet of aluminium foil and the THF is heated under a reflux condenser. After 40 hours, 0.4 g (0.33 mmol, 3% eq) of Pd(PPh3)4 catalyst is added and the reaction of THF under a reflux condenser for 24 hours is continued. The mixture is cooled to room temperature, then allowed to settle before filtering the resin on a Buchner funnel. The resin is washed successively with THF (4×30 ml), a 1% solution of diethyldithiocarbamic acid sodium salt in DMF (4×30 ml), a 1:1 mixture of THF/H2O (4×30 ml), then H2O (2×30 ml), MeOH (2×30 ml), DMF (2×30 ml), MeOH (2×30 ml), 3×[3×30 ml CH2Cl2, 3×30 ml MeOH] and, lastly, dried under vacuum (dessicator). 5.2 g of a light orange powder (yield: 70%) is obtained.

[0132] IR (KBr, cm−1): 1730 (CO)

Elemental analysis:
		Calc.	Exp.
	% N	2.8	1.95

EXAMPLE 8

tert-butyl-N-(2-ethoxyphenyl)carbamate Bonded on Solid Support 9

[0133] In a 500 ml three-necked flask under an argon atmosphere, 8 g (23.8 mmol, 1.7 eq) of tert-butyl-N-{4-[allyl(dimethyl)silyl]-2-ethoxyphenyl}carbamate 7 is mixed with 100 ml of anhydrous THF. Then 42.8 ml (21.4 mmol, 1.5 eq) of 0.5 M 9-BBN is added in anhydrous THF. The reaction is allowed to progress at room temperature and under argon for 4 hours. Then 0.5 g (0.42 mmol, 3% eq) of Pd(PPh3)4, 12 ml (1.7 eq) of an aqueous 2 M Na2CO3 solution, and 3.5 g (14 mmol) of bromopolystyrene resin (substituted 4 mmol/g) is added. The flask is wrapped in a sheet of aluminium foil and the THF is heated under a reflux condenser. After 40 hours, 0.5 g (0.42 mmol, 3% eq) of Pd(PPh3)4 catalyst is added and the reaction of THF under a reflux condenser for 24 hours is continued. The mixture is cooled to room temperature, then allowed to settle before filtering the resin on a Buchner funnel. The resin is washed successively with THF (4×40 ml), a 1% solution of diethyldithiocarbamic acid sodium salt in DMF (4×40 ml), a 1:1 mixture of THF/H2O (4×40 ml), then H2O (2×40 ml), MeOH (2×40 ml), DMF (2×40 ml), MeOH (2×40 ml), 3×[3×40 ml CH2Cl2, 3×40 ml MeOH] and, lastly, dried under vacuum (dessicator). 5.86 g of a light orange powder (yield: 80%) is obtained.

[0134] IR (cm−1): 1729 (CO)

Elemental analysis:
		Calc.	Exp.
	% N	2.76	2.23

EXAMPLE 9

[0135] 2-methoxyaniline Bonded on Solid Support 10

[0136] 1 g (2 mmol) of tert-butyl-N-(2-methoxyphenyl)carbamate (2 mmol/g) bonded on solid support 8 is reacted with 1.2 g (8 mmol, 4 eq) of a 0.2 M solution of B-chlorocatecholborane in anhydrous CH2Cl2, the mixture is shaken at room temperature and under argon for 10 min. Next, 16 ml of water is added and shaken for an additional 20 min. Then the resin is filtered on a scintered filter and rinsed successively with 10% NaOH (60 ml), H2O, DMF, MeOH, CH2Cl2, MeOH. After drying the resin in a dessicator, 0.8 g of a brown powder is obtained (yield: 98%).

[0137] IR (KBr, cmd): disappearance of the CO band at 1730 cm−1

Elemental analysis:
		Calc.	Exp.
	% N	2.24	2.21

EXAMPLE 10

2-ethoxyaniline Bonded on Solid Support (11)

[0138] The reaction between 1.2 g (1.4 mmol) of tert-butyl-N-(2-ethoxyphenyl)carbamate (˜1.2 mmol/g) bonded on solid support 9 and 0.87 g (5.66 mmol, 4 eq) of 0.2 M B-chlorocatecholborane in anhydrous CH2Cl2 according to the procedure described hereinabove, followed by filtration and washing, gave 1 g of a brown powder (yield: 95

[0139] IR (cm−1): disappearance of the CO band at 1729 cm−1

Elemental analysis:
		Calc.	Exp.
	% N	1.88	1.78

EXAMPLE 11

Cleavage of tert-butyl-N-(2-methoxyphenyl)carbamate Bonded on Solid Support (8)

[0140] 0.35 g of tert-butyl-N-(2-methoxyphenyl)carbamate (theoretically 2 mmol/g) bonded on solid support 8 is reacted with 0.5 ml (7 mmol, 10 eq) of a 20% solution of TFA/CH2Cl2 at room temperature for 1 hour. The resin is filtered on a scintered filter and rinsed with CH2Cl2 and MeOH. Then, the filtrate is concentrated under vacuum, taken up in CH2Cl2, washed with a saturated aqueous NaHCO3 solution. After drying on MgSO4 and evaporation of the solvent, 31.1 mg of 2-methoxyaniline is obtained and characterized by the 1H NMR spectrum and compared with the commercial product (yield: 36%). An IR control of the resin showed that the cleavage was not complete (presence of a carbonyl band at 1729 cm−1). The resin was then reacted again with 10 equivalents of a 20% solution of TFA/CH2Cl2 for 15 hours at room temperature. After an identical treatment, 13.8 g of pure 2-methoxyaniline were obtained (overall yield: 52%).

EXAMPLE 12

2-bromoethyl-N-(2-methoxyphenyl)Carbamate (12)

[0141] In a 50 ml two-necked flask placed under an argon atmosphere, 1.5 g (7 mmol, 1.4 eq) of (Boc)2O is dissolved in 5 ml of anhydrous CH2Cl2. 61 mg (0.5 mol, 10% eq) of DMAP dissolved in 5 ml of anhydrous CH2Cl2 is added, then 0.56 ml (5 mmol) of 2-methoxyaniline is added dropwise. The mixture is stirred at room temperature for 20 min before adding 0.5 ml (7 mmol, 1.4 eq) of 2-bromoethanol diluted in 5 ml of anhydrous CH2Cl2. The reaction is allowed to progress for 30 min at room temperature then for approximately 20 hours under reflux of dichlormethane. Then, the medium is cooled and concentrated under vacuum. The resulting dark pink solid is purified by chromatography on a silica gel column (eluent CH2Cl2) to give 1.33 g of a colorless oil (yield: 95%). 11

[0142] C10H12BrNO3

[0143] M=274.12 g/mol

[0144] 1 H NMR (CDCl3) δ (ppm): 8.05 (d, J=7.6 Hz, 1H, CH(6)); 7.32 (sl, 1H, NH); 7.02-6.84 (m, 3H, CH(3), CH(4), CH(5)); 4.47 (t, J=6 Hz, 2H, CH2O); 3.86 (s, 3H, OCH3); 3.57 (t, J=6 Hz, 2H, CH2Br).

[0145] 13 C NMR (CDCl3) δ (ppm): 152.6 (C); 147.6 (C2); 127.2 (C,); 123 (C4); 121 (C6); 118.2 (C5); 110 (C3); 64.4 (CH2O); 55.6 (OCH3); 29.1 (CH2Br).

[0146] GC (Rt, min, 70° C., 20° C./min): 6.92.

Elemental analysis:
		Calc.	Exp.
	% C	43.82	43.71
	% H	4.41	4.52
	% N	5.11	5.02

[0147] 2-bromoethyl-N-(2-methoxyphenyl)carbamate (12)

[0148] The reaction between 0.15 g (0.17 mmol) of 2-methoxyaniline resin 10 (˜1.11 mmol/g), 0.25 g (1.2 mmol, 7 eq) of (Boc)2O, 0.1 g (0.9 mmol, 5 eq) of DMAP, and 85 μl (1.2 mmol, 7 eq) of 2-bromoethanol in 6 to 8 ml of CH2Cl2 at room temperature for 16 hours according to the procedure described in example 12, followed by filtration and washing of the resin, yields an orange powder. Cleavage of the resin is carried out in 2 ml of TFA for 2 hours at room temperature. The resin is filtered and washed with CH2Cl2 and MeOH, and the filtrate is concentrated under vacuum. 27.3 mg of a brown oil (crude yield: 66%) is obtained. The composition of the crude mixture is analyzed by GC. The following products, among others, are obtained: 2-methoxyphenylaniline (15%), isocyanate intermediate (7%), 2-chloroethyl-N-(2-methoxyphenyl)carbamate (5.2%) and 2-bromoethyl-N-(2-methoxyphenyl)carbamate 12 (59.4%).

[0149] GC/MS (Rt, min, 90° C., 30 s, 10° C./min, 220° C.; IE 70 eV): 5.62 (2-methoxyaniline, M=123), 6.07 (isocyanate, M=149), 14.00 (2-chloroethyl-N-(2-methoxyphenyl)carbamate, M=229) and 15.24 (12.

EXAMPLE 13

Preparation of a Library of Carbamates Represented by Formula (I)

[0150] wherein R1=OEt, Y═(CH2)m, mr=2 or 3, G=J=CH, n=1

[0151] An average mass of 90 mg of 2-ethoxyaniline resin 11 (˜1.8 mmol/g) is weighed into 48 polypropylene reactors which are placed on the reaction block mounted on a Fisher Innova 2100 orbital shaker.

[0152] First Step: Formation of Carbamates Bonded to the Resin

[0153] 4 ml of a solution prepared from 14.85 g (0.28 mol/l, 7 eq) of (Boc)2O and 5.94 g (0.20 mol/l, 5 eq) of DMAP dissolved in 240 ml of CH2Cl2 are distributed into each reactor. The reaction block is shaken for 30 min. To the first 24 reactors (matrix 1 to 24) 2 ml of a solution of 3.32 ml (0.56 mol/l, 7 eq) of 2-bromoethanol diluted in 70 ml of CH2Cl2 is addded and to the last 24 reactors (matrix 25 to 48) 2 ml of a solution of 3.55 ml (0.56 mmol/l, 7 eq) of 3-bromopropanol diluted in 70 ml of CH2Cl2 is added. The reaction block is shaken at room temperature for 16 hours. The resins are filtered, then rinsed three times each with three successive solvents: DMF, MeOH, CH2Cl2 (4 ml/reactor per rinse). The resin is allowed to dry in air.

[0154] Second Step: N-alkylation Reaction

[0155] 1.5 ml of a corresponding piperazine solution (1.6 mmol, 10 eq) previously prepared by dissolving the quantity to be weighed in 3.3 ml of CH2Cl2 is distributed into each reactor. A slight excess of each solution is prepared. The piperazines used and the amounts added in the reaction are listed below:

Structure	MW	Quantity (g)
12	162.24	0.578
13	192.26	0.685
14	192.26	0.685
15	192.26	0.685
16	196.68	0.700
17	196.68	0.700
18	196.68	0.700
19	176.26	0.628
20	176.26	0.628
21	176.26	0.628
22	180.23	0.642
23	180.23	0.642
24	230.23	0.820
25	163.22	0.581
26	163.22	0.581
27	176.26	0.628
28	164.21	0.585
29	164.21	0.585
30	164.21	0.585
31	164.21	0.585
32	198.66	0.708
33	198.66	0.708
34	190.29	0.678
35	178.24	0.635

[0156] Next, 125 μl (4.5 eq) of DIEA to each reactor and shake the mixture at room temperature for 40 hours is added. The resins are filtered and rinsed three times each with three successive solvents: DMF, MeOH, CH2Cl2 (4 ml/reactor per rinse). The resin is allowed to dry in air.

[0157] Cleavage

[0158] Cleavage is carried out in 2 ml of TFA per reactor at room temperature for 2 hours. The resins are filtered and rinsed with 2×2 ml of CH2Cl2, and the filtrates are concentrated under vacuum.

[0159] Extraction in Alkaline Medium

[0160] The 48 mixtures are individually taken up in 8 ml of a 1:1 mixture of CH2Cl2/H2O in Whatman 12 ml cartridges equipped with a PTFE filter. The organic phase (=Organic phase A) is filtered, the aqueous phase is washed with 4 ml of CH2Cl2. The pH of the aqueous phase is adjusted to alkaline pH range by adding a saturated aqueous Na2CO3 solution. Then the aqueous phase is extracted with CH2Cl2 (1×4 ml then 1×2 ml). The 48 filtrates (=48 organic phase B) are vacuum concentrated.

[0161] Purification on Cation Exchange Resin

[0162] The cation exchange resin is a BCX resin packaged by Bodhan (Mettler-Toledo) in the form of 1 g SPE cartridges. The resin is first washed with 2×3 ml of MeOH (conditioning). The crude mixture, dissolved in 1 ml of MeOH and adjusted to pH 9 with a 1 M aqueous NaOH solution, is then deposited on the small resin column (loading). The column is then washed with 2×3 ml of MeOH (wash). The piperazine derivative is then released by elution with 3 ml of a 2 M solution of NH4OH/MeOH (elution). The filtrate is concentrated under vacuum.

[0163] The resulting compounds represented by formula (I) are described and characterized as follows:

[0164] 2-[4-(2-methoxyphenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 1436

[0165] C22H29N3O4

[0166] M=399.49 g/mol

[0167] Quantity: 2.2 mg (yield: 3%)

[0168] HPLC: t=2.45 min

[0169] MS: 400.68 (MH+)

[0170] HPLC purity: 85%

[0171] 2-[4-(3-methoxyphenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 15

[0172] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 37

[0173] C22H29N3O4

[0174] M=399.49 g/mol

[0175] Quantity: 6.8 mg (yield: 10%)

[0176] HPLC: t=2.64 min

[0177] MS: 400.51 (MH+)

[0178] HPLC purity: 94%

[0179] 2-[4-(4-methoxyphenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 1638

[0180] C22H29N3O4

[0181] M=399.49 g/mol

[0182] Quantity: 3.4 mg (yield: 5%)

[0183] HPLC: t=2.39 min

[0184] MS: 400.68 (MH+)

[0185] HPLC purity: 86%

[0186] 2-[4-(2-chlorophenyl)piperazino]ethyl N-(2-ethoxyphenyl)carbamate 1739

[0187] C21H26ClN3O3

[0188] M=403.91 g/mol

[0189] Quantity: 1.7 mg (yield: 2%)

[0190] HPLC: t=2.64 min

[0191] MS: 404.63 (MH+)

[0192] HPLC purity: 77%

[0193] 2-[4-(3-chlorophenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 18

[0194] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 40

[0195] C21H26ClN3O3

[0196] M=403.91 g/mol

[0197] Quantity: 8.5 mg (yield: 13%)

[0198] HPLC: t=2.85 min

[0199] MS: 404.46 (MH+)

[0200] HPLC purity: 96%

[0201] 2-[4-(4-chlorophenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 19

[0202] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) ultimately leads to the expected compound. 41

[0203] C21H26ClN3O3

[0204] M=403.91 g/mol

[0205] Quantity: 7.3 mg (yield: 11%)

[0206] HPLC: t=2.85 min

[0207] MS: 404.47 (MH+)

[0208] HPLC purity: 85%

[0209] 2-[4-(2-methylphenyl)pipeazino]ethyl-N-(2-ethoxyphenyl)carbamate 20

[0210] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) ultimately leads to the expected compound. 42

[0211] C22H29N3O3

[0212] M=383.49 g/mol

[0213] Quantity: 7.1 mg (yield: 11%)

[0214] HPLC: t=2.83 min

[0215] MS: 384.51 (MH+)

[0216] HPLC purity: 74%

[0217] 2-[4-(3-methylphenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 2143

[0218] (1NHeo YN

[0219] C22H29N3O3

[0220] CH3 M=383.49 g/mol

[0221] Quantity: 3.8 mg (yield: 6%)

[0222] HPLC: t=2.73 min

[0223] MS: 384.59 (MH+)

[0224] HPLC purity: 93%

[0225] 2-[4-(4-methylphenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 2244

[0226] C22H29N3O3

[0227] M=383.49 g/mol

[0228] Quantity: 3.2 mg (yield: 5%)

[0229] HPLC: t=2.76 min

[0230] MS: 384.60 (MH+)

[0231] HPLC purity: 56%

[0232] 2-[4-(2-fluorophenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 23

[0233] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) ultimately leads to the expected compound. 45

[0234] C21H26FN3O3

[0235] M=387.46 g/mol

[0236] Quantity: 10.2 mg (yield: 16%)

[0237] HPLC: t=2.63 min

[0238] MS: 388.49 (MH+)

[0239] HPLC purity: 93%

[0240] 2-[4-(2-pyridinyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 2446

[0241] C20H26N4O3

[0242] M=370.46 g/mol

[0243] Quantity: 7.6 mg (yield: 12%)

[0244] HPLC: t=1.81 min

[0245] MS: 371.56 (MH+)

[0246] HPLC purity: 55%

[0247] 2-[4-(3-pyridazinyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 2547

[0248] C19H25N5O3

[0249] M=371.44 g/mol

[0250] Quantity: 4.3 mg (yield: 7%)

[0251] HPLC: t=1.83 min

[0252] MS: 372.55 (MH+)

[0253] HPLC purity: 27%

[0254] 2-[4-(2-pyrimidinyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 26

[0255] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 48

[0256] C19H25N5O3

[0257] M=371.44 g/mol

[0258] Quantity: 10.1 mg (yield: 16%)

[0259] HPLC: t=2.26 min

[0260] MS: 372.49 (MH+)

[0261] HPLC purity: 94%

[0262] 2-[4-(6-2hloro-3-pyridazinyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 27

[0263] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 49

[0264] C19H24ClN5O3

[0265] M=405.89 g/mol

[0266] Quantity: 6.8 mg (yield: 10%)

[0267] HPLC: t=2.27 min

[0268] MS: 406.47 (MH+)

[0269] HPLC purity: 89%

[0270] 2-[4-(6-methyl-3-pyridazinyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 2850

[0271] C20H27N5O3

[0272] M=385.47 g/mol

[0273] Quantity: 7 mg (yield: 11%)

[0274] HPLC: t=2.13 min

[0275] MS: 386.58 (MH+)

[0276] HPLC purity: 61%

[0277] 3-[4-(2-methoxyphenyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 29

[0278] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 51

[0279] C23H31N3O4

[0280] M=413.52 g/mol

[0281] Quantity: 14.7 mg (yield: 22%)

[0282] HPLC: t=2.57 min

[0283] MS: 414.55 (MH+)

[0284] HPLC purity: 100%

[0285] 3-[4-(3-methoxyphenyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 30

[0286] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 52

[0287] C23H31N3O4

[0288] M=413.52 g/mol

[0289] Quantity: 11.5 mg (yield: 17%)

[0290] HPLC: t=2.62 min

[0291] MS: 414.55 (MH+)

[0292] HPLC purity: 98%

[0293] 3-[4-(2-chlorophenyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 31

[0294] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 53

[0295] C22H28ClN3O3

[0296] M=417.94 g/mol

[0297] Quantity: 14.1 mg (yield: 20%)

[0298] HPLC: t=2.79 min

[0299] MS: 418.50 (MH+)

[0300] HPLC purity: 94%

[0301] 3-[4-(3-chlorophenyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 32

[0302] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 54

[0303] C22H28ClN3O3

[0304] M=417.94 g/mol

[0305] Quantity: 13.3 mg (yield: 19%)

[0306] HPLC: t=2.83 min

[0307] MS: 418.51 (MH+)

[0308] HPLC purity: 100%

[0309] 3-[4-(4-chlorophenyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 33

[0310] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 55

[0311] C22H28ClN3O3

[0312] M=417.94 g/mol

[0313] Quantity: 0 mg (yield: 14%)

[0314] HPLC: t=2.87 min

[0315] MS: 418.50 (MH+)

[0316] HPLC purity: 94%

[0317] 3-[4-(2-methylphenyl)piperazino]propyl N-(2-ethoxyphenyl)carbamate 34

[0318] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 56

[0319] C23H31N3O3

[0320] M=397.52 g/mol

[0321] Quantity: 7.2 mg (yield: 11%)

[0322] HPLC: t=2.87 min

[0323] MS: 398.55 (MH+)

[0324] HPLC purity: 100%

[0325] 3-[4-(3-methylphenyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 35

[0326] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 57

[0327] C23H3N3O3

[0328] M=397.52 g/mol

[0329] Quantity: 10.7 mg (yield: 16%)

[0330] HPLC: t=2.80 min

[0331] MS: 398.55 (MH+)

[0332] HPLC purity: 95%

[0333] 3-[4-(4-methylphenyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 36

[0334] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 58

[0335] C23H31N3O3

[0336] M=397.52 g/mol

[0337] Quantity: 8.7 mg (yield: 13%)

[0338] HPLC: t=2.77 min

[0339] MS: 398.56 (MH+)

[0340] HPLC purity: 90%

[0341] 3-[4-(2-fluorophenyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 3759

[0342] C22H28FN3O3

[0343] M=401.49 g/mol

[0344] Quantity: 1 mg (yield: 1%)

[0345] HPLC: t=2.71 min

[0346] MS: 402.57 (MH+)

[0347] HPLC purity: 44%

[0348] 3-[4-(4-fluorophenyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 38

[0349] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 60

[0350] C22H28FN3O3

[0351] M=401.49 g/mol

[0352] Quantity: 11.9 mg (yield: 18%)

[0353] HPLC: t=2.66 min

[0354] MS: 402.53 (MH+)

[0355] HPLC purity: 82%

[0356] 3-[4-[3-(trifluoromethyl)phenyl]piperazino]propyl-N-(2-ethoxyphenyl)carbamate 3961

[0357] C23H28F3N3O3

[0358] M=451.49 g/mol

[0359] Quantity: 50.5 mg (yield: 69%)

[0360] HPLC: t=2.87 min

[0361] MS: 452.52 (MH+)

[0362] HPLC purity: 85%

[0363] 3-[4-(2-pyridinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 4062

[0364] C21H28N4O3

[0365] M=384.48 g/mol

[0366] Quantity: 6 mg (yield: 9%)

[0367] HPLC: t=1.98 min

[0368] MS: 385.56 (MH+)

[0369] HPLC purity: 100%

[0370] 3-[4-(4-pyridinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 4163

[0371] C21H28N4O3

[0372] M=384.48 g/mol

[0373] Quantity: 8.7 mg (yield: 14%)

[0374] HPLC: t=1.90 min

[0375] MS: 385.56 (MH+)

[0376] HPLC purity: 100%

[0377] 3-[4-(3-pyridazinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 4264

[0378] C20H27N5O3

[0379] M=385.47 g/mol

[0380] Quantity: 6.1 mg (yield: 9%)

[0381] HPLC: t=1.94 min

[0382] MS: 386.56 (MH+)

[0383] HPLC purity: 96%

[0384] 3-[4-(4-pyrimidinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 4365

[0385] C20H27N5O3

[0386] M=385.47 g/mol

[0387] Quantity: 8.5 mg (yield: 13%)

[0388] HPLC: t=1.86 min

[0389] MS: 386.55 (MH+)

[0390] HPLC purity: 100%

[0391] 3-[4-(2-pyrimidinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 44

[0392] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 66

[0393] C20H27N5O3

[0394] M=385.47 g/mol

[0395] Quantity: 16.9 mg (yield: 27%)

[0396] HPLC: t=2.27 min

[0397] MS: 386.52 (MH+)

[0398] HPLC purity: 97%

[0399] 3-[4-(2-pyrazinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 4567

[0400] C20H27N5O3

[0401] M=385.47 g/mol

[0402] Quantity: 2.3 mg (yield: 3%)

[0403] HPLC: t=2.19 min

[0404] MS: 386.55 (MH+)

[0405] HPLC purity: 79%

[0406] 3-[4-(6-chloro-3-pyridazinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 46

[0407] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 68

[0408] C20H26ClN5O3

[0409] M=419.91 g/mol

[0410] Quantity: 10.7 mg (yield: 15%)

[0411] HPLC: t=2.32 min

[0412] MS: 420.50 (MH+)

[0413] HPLC purity: 100%

[0414] 3-[4-(2,5-dimethylphenyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 47

[0415] Purification on a silica gel column (eluent AcOEt/petroleum ether 8:2 then AcOEt/petroleum ether/NH4OH 8:2:0.2) leads to the expected compound. 69

[0416] C24H33N3O3

[0417] M=411.55 g/mol

[0418] Quantity: 7.5 mg (yield: 11%)

[0419] HPLC: t=3.02 min

[0420] MS: 412.58 (MH+)

[0421] HPLC purity: 85%

[0422] 3-[4-(6-methyl-3-pyridazinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 4870

[0423] C21H29N5O3

[0424] M=399.50 g/mol

[0425] Quantity: 7.7 mg (yield: 11%)

[0426] HPLC: t=1.88 min

[0427] MS: 400.58 (MH+)

[0428] HPLC purity: 100%

EXAMPLE 14

Preparation of a Library of Carbamates Represented by Formula (I)

[0429] wherein

R1=OMe, Y═(CH2)m, m=2 or 3, G=J=CH, n=1

[0430] An average mass of 90 mg of 2-methoxyaniline resin 10 (˜1.3 mmol/g) is weighed into 44 polypropylene reactors which are placed on the reaction block mounted on the orbital shaker.

[0431] First Step: Formation of Carbamates

[0432] 4 ml of a solution prepared from 9.82 g (0.20 mol/l, 7 eq) of (Boc)2O and 3.93 g (0.14 mol/l, 5 eq) of DMAP dissolved in 220 ml of CH2Cl2 are distributed into each reactor. The reaction block is shaken for 30 min. To the first 22 reactors 2 ml of a solution of 4.8 ml (82 mmol/l, 14 eq) of 2-bromoethanol diluted in 70 ml of CH2Cl2 is added, and to the last 22 reactors 2 ml of a solution of 5.2 ml (82 mmol/l, 14 eq) of 3-bromopropanol diluted in 70 ml of CH2Cl2 is added. The reaction block is shaken at room temperature for 28 hours. The shaker is stopped. The resins are filtered then rinsed three times each with three successive solvents: DMF, MeOH, CH2Cl2 (4 ml/reactor per rinse). The resin is allowed to dry in air.

[0433] Second Step: N-Alkylation Reaction

[0434] 1.2 ml of a corresponding piperazine solution (1.2 mmol, 10 eq) previously prepared is distributed into each reactor. The piperazines used and the amounts added to the reaction are listed below:

Structure	MW	Quantity (g)
71	162.24	0.417
72	192.26	0.494
73	192.26	0.494
74	192.26	0.494
75	196.68	0.506
76	196.68	0.506
77	196.68	0.506
78	176.26	0.453
79	176.26	0.453
80	176.26	0.453
81	180.23	0.463
82	180.23	0.463
83	230.23	0.592
84	163.22	0.420
85	163.22	0.420
86	176.26	0.453
87	164.21	0.422
88	164.21	0.422
89	164.21	0.422
90	198.66	0.511
91	198.66	0.511
92	190.29	0.489

[0435] Next, 75 μl (3.5 eq) of DIEA is added to each well and shaken at room temperature for 40 hours. The resins are filtered and rinsed three times each with three successive solvents: DMF, MeOH, CH2Cl2 (4 ml/reactor per rinse). The resin is allowed to dry in air.

[0436] Cleavage

[0437] Cleavage is carried out in 2 ml of TFA per reactor at room temperature for 2 hours. The resins are filtered and rinsed with 2×2 ml of CH2Cl2, then the filtrates are concentrated. Each filtrate is taken up in 1 ml of MeOH, the pH is adjusted to 9 with an aqueous 1 M NaOH solution. The mixtures are then purified on cation exchange resins according to the protocol described hereinabove. The 44 filtrates collected into previously tared tubes are vacuum concentrated (Genevac) and analyzed by HPLC/MS before being weighed.

[0438] 2-(4-phenylpiperazino)ethyl-N-(2-methoxyphenyl)carbamate 4993

[0439] C20H25N3O3

[0440] M=355.44 g/mol

[0441] Quantity: 4.4 mg (yield: 10%)

[0442] HPLC: t=2.41 min

[0443] MS: 356.46 (MH+)

[0444] HPLC purity: 85%

[0445] 2-[4-(2-methoxyphenyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 5094

[0446] C21H27N3O4

[0447] M=385.47 g/mol

[0448] Quantity: 3.5 mg (yield: 7%)

[0449] HPLC: t=2.44 min

[0450] MS: 386.49 (MH+)

[0451] HPLC purity: 99%

[0452] 2-[4-(3-methoxyphenyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 5195

[0453] C21H27N3O4

[0454] M=385.47 g/mol

[0455] Quantity: 2.8 mg (yield: 6%)

[0456] HPLC: 2.46 min

[0457] MS: 386.49 (MH+)

[0458] HPLC purity: 92%

[0459] 2-[4-(4-methoxyphenyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 5296

[0460] C21H27N3O4

[0461] M=385.47 g/mol

[0462] Quantity: 7.9 mg (yield: 17%)

[0463] HPLC: t=2.39 min

[0464] MS: 386.49 (MH+)

[0465] HPLC purity: 88%

[0466] 2-[4-(2-chlorophenyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 5397

[0467] C20H24ClN3O3

[0468] M=389.89 g/mol

[0469] Quantity: 5.6 mg (yield: 12%)

[0470] HPLC: t=2.62 min

[0471] MS: 390.44 (MH+)

[0472] HPLC purity: 97%

[0473] 2-[4-(3-chlorophenyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 5498

[0474] C20H24ClN3O3

[0475] M=389.89 g/mol

[0476] Quantity: 3.1 mg (yield: 6%)

[0477] HPLC: t=2.70 min

[0478] MS: 390.44 (MH+)

[0479] HPLC purity: 91%

[0480] 2-[4-(4-chlorophenyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 5599

[0481] C20H24ClN3O3

[0482] M=389.89 g/mol

[0483] Quantity: 6.4 mg (yield: 14%)

[0484] HPLC: 2.69 min

[0485] MS: 390.44 (MH+)

[0486] HPLC purity: 78%

[0487] 2-[4-(2-methylphenyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 56100

[0488] C21H27N3O3

[0489] M=369.47 g/mol

[0490] Quantity: 8.4 mg (yield: 19%)

[0491] HPLC: t=2.61 min

[0492] MS: 370.49 (MH+)

[0493] HPLC purity: 84%

[0494] 2-[4-(3-methylphenyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 57101

[0495] C21H27N3O3

[0496] M=369.47 g/mol

[0497] Quantity: 4.1 mg (yield: 9%)

[0498] HPLC: t=2.59 min

[0499] MS: 370.50 (MH+)

[0500] HPLC purity: 93%

[0501] 2-[4-(4-methylphenyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 58102

[0502] C21H27N3O3

[0503] M=369.47 g/mol

[0504] Quantity: 4.8 mg (yield: 11%)

[0505] HPLC: t=2.61 min

[0506] MS: 370.50 (MH+)

[0507] HPLC purity: 88%

[0508] 2-[4-(2-fluorophenyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 59103

[0509] C20H24FN3O3

[0510] M=373.43 g/mol

[0511] Quantity: 4.8 mg (yield: 11%)

[0512] HPLC: t=2.50 min

[0513] MS: 374.48 (MH+)

[0514] HPLC purity: 96%

[0515] 2-[4-(4-fluorophenyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 60104

[0516] C20H24FN3O3

[0517] M=373.43 g/mol

[0518] Quantity: 1.6 mg (yield: 3%)

[0519] HPLC: t=2.52 min

[0520] MS: 374.48 (MH+)

[0521] HPLC purity: 79%

[0522] 2-[4-3-(trifluoromethyl)phenyl]piperazino-ethyl-N-(2-methoxyphenyl)carbamate 61105

[0523] C20H24F3N3O3

[0524] M=423.44 g/mol

[0525] Quantity: 4.2 mg (yield: 8%)

[0526] HPLC: t=2.83 min

[0527] MS: 424.50 (MH+)

[0528] HPLC purity: 91%

[0529] 2-[4-(2-pyridinyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 62106

[0530] C19H24N4O3

[0531] M=356.43 g/mol

[0532] Quantity: 5.3 mg (yield: 12%)

[0533] HPLC: t=2.03 min

[0534] MS: 357.48 (MH+)

[0535] HPLC purity: 80%

[0536] 2-[4-(4-pyridinyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 63107

[0537] C19H24N4O3

[0538] M=356.43 g/mol

[0539] Quantity: 1.8 mg (yield: 4%)

[0540] HPLC: t=2.08 min

[0541] MS: 357.48 (MH+)

[0542] HPLC purity: 83%

[0543] 2-[4-(3-pyridazinyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 64108

[0544] C18H23N5O3

[0545] M=357.42 g/mol

[0546] Quantity: 2.9 mg (yield: 7%)

[0547] HPLC: t=1.97 min

[0548] MS: 358.48 (MH+)

[0549] HPLC purity: 87%

[0550] 2-[4-(2-pyrimidinyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 65109

[0551] C18H23N5O3

[0552] M=357.42 g/mol

[0553] Quantity: 4.3 mg (yield: 10%)

[0554] HPLC: t=2.09 min

[0555] MS: 358.48 (MH+)

[0556] HPLC purity: 99%

[0557] 2-[4-(2-pyrazinyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 66110

[0558] C18H23N5O3

[0559] M=357.42 g/mol

[0560] Quantity: 4.5 mg (yield: 10%)

[0561] HPLC: t=1.99 min

[0562] MS: 358.48 (MH+)

[0563] HPLC purity: 97%

[0564] 2-[4-t3-(6-chloro)pyridazinylpiperazino]ethyl-N-(2-methoxyphenyl)carbamate 67111

[0565] C18H22ClNsO3

[0566] M=391.86 g/mol

[0567] Quantity: 5.1 mg (yield: 11%)

[0568] HPLC: t=2.11 min

[0569] MS: 392.46 (MH+)

[0570] HPLC purity: 80%

[0571] 2-[4-(2,5-dimethylphenyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 68112

[0572] C22H29N3O3

[0573] M=383.49 g/mol

[0574] Quantity: 4.8 mg (yield: 10%)

[0575] HPLC: t=2.8 min

[0576] MS: 384.54 (MH+)

[0577] HPLC purity: 93%

[0578] 3-(4-phenylpiperazino)propyl-N-(2-methoxyphenyl)carbamate 69113

[0579] C21H27N3O3

[0580] M=369.47 g/mol

[0581] Quantity: 14.1 mg (yield: 32%)

[0582] HPLC: t=2.39 min

[0583] MS: 370.52 (MH+)

[0584] HPLC purity: 98%

[0585] 3-[4-(2-methoxyphenyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 70114

[0586] C22H29N3O4

[0587] M=369.47 g/mol

[0588] Quantity: 15.3 mg (yield: 32%)

[0589] HPLC: t=2.42 min

[0590] MS: 370.52 (MH+)

[0591] HPLC purity: 98%

[0592] 3-[4-(3-methoxyphenyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 71115

[0593] C22H29N3O4

[0594] M=369.47 g/mol

[0595] Quantity: 8.9 mg (yield: 19%)

[0596] HPLC: t=2.48 min

[0597] MS: 370.52 (MH+)

[0598] HPLC purity: 89%

[0599] 3-[4-(4-methoxyphenyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 72116

[0600] C22H29N3O4

[0601] M=369.47 g/mol

[0602] Quantity: 15.2 mg (yield: 32%)

[0603] HPLC: t=2.31 min

[0604] MS: 370.52 (MH+)

[0605] HPLC purity: 98%

[0606] 3-[4-(2-chlorophenyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 73117

[0607] C21H26ClN3O3

[0608] M=403.91 g/mol

[0609] Quantity: 14.5 mg (yield: 30%)

[0610] HPLC: t=2.59 min

[0611] MS: 404.51 (MH+)

[0612] HPLC purity: 98%

[0613] 3-[4-(3-chlorophenyl)piperazino]propyl N-(2-methoxyphenyl)carbamate 74118

[0614] C21H26ClN3O3

[0615] M=403.91 g/mol

[0616] Quantity: 13.1 mg (yield: 27%)

[0617] HPLC: t=2.63 min

[0618] MS: 404.50 (MH+)

[0619] HPLC purity: 97%

[0620] 3-[4-(4-chlorophenyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 75119

[0621] C21H26ClN3O3

[0622] M=403.91 g/mol

[0623] Quantity: 10 mg (yield: 21%)

[0624] HPLC: t=2.61 min

[0625] MS: 404.50 (MH+)

[0626] HPLC purity: 96%

[0627] 3-[4-(2-methylphenyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 76120

[0628] C22H29N3O3

[0629] M=383.49 g/mol

[0630] Quantity: 8.8 mg (yield: 19%)

[0631] HPLC: t=2.70 min

[0632] MS: 384.55 (MH+)

[0633] HPLC purity: 79%

[0634] 3-[4-(3-methylphenyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 77121

[0635] CH3 C22H29N3O3

[0636] M=383.49 g/mol

[0637] Quantity: 9.9 mg (yield: 22%)

[0638] HPLC: t=2.59 min

[0639] MS: 384.55 (MH+)

[0640] HPLC purity: 97%

[0641] 3-[4-(4-methylphenyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 78122

[0642] C22H29N3O3

[0643] M=383.49 g/mol

[0644] Quantity: 13.8 mg (yield: 30%)

[0645] HPLC: t=2.58 min

[0646] MS: 384.55 (MH+)

[0647] HPLC purity: 91%

[0648] 3-[4-(2-fluorophenyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 79123

[0649] C21H26FN3O3

[0650] M=387.46 g/mol

[0651] Quantity: 6.3 mg (yield: 14%)

[0652] HPLC: t=2.55 min

[0653] MS: 388.52 (MH+)

[0654] HPLC purity: 70%

[0655] 3-[4-(4-fluorophenyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 80124

[0656] C21H26FN3O3

[0657] M=387.46 g/mol

[0658] Quantity: 10.1 mg (yield: 22%)

[0659] HPLC: t=2.50 min

[0660] MS: 388.53 (MW+)

[0661] HPLC purity: 92%

[0662] 3-{4-[3-(trifluoromethyl)phenyl]piperazino}propyl-N-(2-methoxyphenyl)carbamate 125

[0663] C22H26F3N3O3

[0664] M=437.47 g/mol

[0665] Quantity: 10.9 mg (yield: 21%)

[0666] HPLC: t=2.84 min

[0667] MS: 438.55 (MH+)

[0668] HPLC purity: 90%

[0669] 3-[4-(2-pyridinyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 82126

[0670] C22H26N4O3

[0671] M=370.46 g/mol

[0672] Quantity: 9.5 mg (yield: 22%)

[0673] HPLC: t=2.03 min

[0674] MS: 371.53 (MH+)

[0675] HPLC purity: 89%

[0676] 3-[4-(4-pyridinyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 83127

[0677] C22H26N4O3

[0678] M=370.46 g/mol

[0679] Quantity: 4.4 mg (yield: 10%)

[0680] RPLC: t=2.11 min

[0681] MS: 371.52 (MH+)

[0682] HPLC purity: 76%

[0683] 3-[2-(4-benzylpiperazino)lpropyl-N-(2-methoxyphenyl)carbamate 84128

[0684] C22H29N3O3

[0685] M=383.49 g/mol

[0686] Quantity: 7.3 mg (yield: 16%)

[0687] HPLC: t=2.61 min

[0688] MS: 384.55 (MH+)

[0689] HPLC purity: 100%

[0690] 3-[4-(3-pyridazinyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 85129

[0691] C19H25N5O3

[0692] M=371.44 g/mol

[0693] Quantity: 4.3 mg (yield: 9%)

[0694] HPLC: t=2.07 min

[0695] MS: 372.51 (MH+)

[0696] HPLC purity: 88%

[0697] 3-L4-(2-pyrimidinyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 86130

[0698] C19H25N5O3

[0699] M=371.44 g/mol

[0700] Quantity: 3.9 mg (yield: 9%)

[0701] HPLC: t=2.13 min

[0702] MS: 372.52 (MH+)

[0703] HPLC purity: 93%

[0704] 3-[4-(2-pyrazinyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 87131

[0705] C19H25N5O3

[0706] M=371.44 g/mol

[0707] Quantity: 4.7 mg (yield: 10%)

[0708] HPLC: t=2.05 min

[0709] MS: 372.52 (MH+)

[0710] HPLC purity: 96%

[0711] 3-[4-(6-chloro-3-pyridazinyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 88132

[0712] C19H24ClN5O3

[0713] M=371.44 g/mol

[0714] Quantity: 8.7 mg (yield: 18%)

[0715] HPLC: t=2.15 min

[0716] MS: 406.50 (MH+)

[0717] HPLC purity: 95%

[0718] 3-[4-(2,5-dimiethylphenyl)piperazino]propyl-N-(2-methoxyphenyl)carbamate 89133

[0719] C23H31N3O3

[0720] M=397.52 g/mol

[0721] Quantity: 6 mg (yield: 13%)

[0722] HPLC: t=2.85 min

[0723] MS: 398.58 (MH+)

[0724] HPLC purity: 92%

EXAMPLE 15

2-(4-phenylpiperazino)ethyl-N-(2-ethoxyphenyl)carbamate 90

[0725] The reaction between 0.8 g (2.8 mmol) of 2-bromoethyl-N-(2-ethoxyphenyl)carbamate, 1 ml (5.6 mmol, 2 eq) of DIEA and 0.5 ml (3.1 mmol, 1.1 eq) of 1-phenylpiperazine in 7 ml of anhydrous acetonitrile, followed by treatment, according to the procedure described in example 16, yields a yellow oil which is purified by column chromatography (eluent AcOEt/petroleum ether 8:2) to produce 0.47 g of a colorless oil which is stored as the hydrochloride. 485 mg of a white powder is obtained (yield: 39%). 134

[0726] C21H27N3O3.2HCl

[0727] M=406.02 g/mol

[0728] F=150-154° C.

[0729] 1 H NMR base (CDCl3) δ (ppm): 8.10 (dd, Jo=6 Hz, Jm=3 Hz, 1H, CH(6)); 7.34-7.22 (m, 3H, CH(3), CH(4), CH(5)); 7.01-6.82 (m, 6H, NH, ArH); 4.35 (t, J=6 Hz, 2H, CH2O); 4.07 (q, J=7 Hz, 2H, OCH2); 3.25-3.20 (m, 4H, CH2(3′), CH2(5′)); 2.78-2.67 (m, 6H, CH2N, CH2(2′), CH2(6′)); 1.43 (t, J=7 Hz, 2H, CH3).

Elemental analysis:
		Calc. (0.3 H2O)	Exp.
	% C	56.25	56.28
	% H	6.66	6.66
	% N	9.37	9.14

EXAMPLE 16

Biological Results

[0730] The compounds of the invention were evaluated by measuring their affinity constant Ki determined by displacing a radioligand [3H]-GR113808 in rat glial cells stably expressing the human isoform h5-HT4e

[0731] Confluent glial cells were washed twice in PBS and centrifuged at 300 g for 5 min. The pellet was used immediately and the cells were suspended in 10 volumes of HEPES (50 mM, pH 7.4, 4° C.) then homogenized with a teflon homogenizer and centrifuged at 40,000 g for 20 min. The pellet was resuspended in 15 volumes of HEPES. Displacement experiments were carried out in 500 μl of buffer (50 mM HEPES) containing 20 μl of radioligand [3H]-GR113808 at a concentration of 0.2 nM for isoform h5-HT4e or at a concentration equal to half of the Kd of the radioligand for the other isoforms expressed in COS cells, 20 μl of competitor ligand at seven different concentrations and 50 μl of membrane preparation (100-200 μg of protein determined by the Bradford method). Binding was carried out at 25° C. for 30 min and the reaction was stopped by rapid vacuum filtration (Brandel Harvester) on Whatman GF/B filters preincubated in 0.1% PEI solution to minimize nonspecific binding.

[0732] Membrane-bound radioactivity is retained on the filter, which was cut and washed with cold buffer (50 mM Tris-HCl, pH 7.4) and incubated overnight in 4 ml of scintillation liquid. Radioactivity was measured in a liquid scintillation counter (Beckman LS 6500C). Binding data were obtained by computer assisted linear regression (Graph Prism Program, Graph Pad Software. Inc., San Diego, Calif.)

[0733] The results in the following table are given as an example:

Compound Ki (nM)
21       20.2
24       4.5
40       9.9
42       3.6
43       10.4
44       11.8
48       3.9
51       12.7
62       9.3
64       10.6
65       10.4
83       10

EXAMPLE 17

Preparation of a Library of Molecules Derived from 2-ethoxyaniline

[0734] 5 μl ofTEA (0.033 mmol, 1.1 eq) is added then 500 μl of piperazine (0.03 mmol, 1 eq) dissolved in DMF to 300 μl of 0.2 M 2-bromoethyl-N-(2-ethoxyphenyl)carbamate (0.06 mmol, 2 eq) dissolved in DMF. After 18 hours at room temperature and evaporation of the solvents, the mixtures are taken up in 1 ml of CH2Cl2 and treated with isocyanate resin (Argonaut, 1.51 mmol/g, 0.09 mmol/g, 0.09 mmol, 3 eq) for 18 hours. Next, the mixtures are purified on cation exchange resins according to the protocol described hereinabove. The desired compounds are then obtained after eliimination of the solvents.

[0735] The compounds in examples 91 to 118 were obtained by the above method. In this table, “Qty” denotes quantity and RT denotes “retention time”.

					RT.
No.	Structure	MW	Qty (mg)	Purity %	(min)	m	Ion
91	135	415.56	2	90	1.87	416.18	M + 1
92	136	384.48	1.9	95	1.4	385.09	M + 1
93	137	395.46	2.1	69	1.67	396.1	M + 1
94	138	445.56	1.3	73	2.05	446.25	M + 1
95	139	532.43	1.9	92	1.83	532.17	M + 1
96	140	404.90	2	94	1.83	405.11	M + 1
97	141	385.46	3.1	100	1.57	386.12	M + 1
98	142	385.46	2.2	93	1.69	386.12	M + 1
99	143	385.46	1.5	81	1.64	386.11	M + 1
100	144	453.56	2	85	1.91	454.19	M + 1
101	145	552.57	2.6	87	1.99	553.18	M + 1
102	146	425.49	1.1	100	1.52	426.18	M + 1
103	147	394.47	2.1	93	1.76	395.11	M + 1
104	148	438.45	2.9	83	1.87	439.21	M + 1
105	149	438.45	2.4	97	1.8	439.24	M + 1
106	150	438.45	2.4	86	1.88	439.22	M + 1
107	151	472.89	2.3	94	1.95	473.11	M + 1
108	152	488.51	2.7	83	1.91	489.17	M + 1
109	153	438.50	2.6	88	1.69	439.16	M + 1
110	154	396.45	2	91	1.65	397.17	M + 1
111	155	427.53	2.5	81	1.59	428.19	M + 1
112	156	408.50	2.7	82	1.74	409.2	M + 1
113	157	481.55	2.4	91	1.87	482.19	M + 1
114	158	535.52	2.5	89	2.01	536.25	M + 1
115	159	502.54	3	80	1.97	503.2	M + 1
116	160	534.58	2.5	82	2.11	535.31	M + 1
117	161	499.56	1.7	68	1.65	500.1	M + 1
118	162	415.45	1.9	82	1.73	416.17	M + 1

[0736] Carbamates Derived from 2-ethoxyaniline

[0737] 3-piperazinobenzonitrile

[0738] 6.7 g (77.66 mmol, 5.5 eq) of piperazine are dissolved in 3 ml of DMSO. 1.5 ml (14.12 mmol) of 3-fluorobenzonitrile is added. The mixture is heated at 100° C. for 60 hours. After cooling to room temperature, the reaction mixture is transferred into 126 ml of water. The precipitate formed is filtered and the filtrate extracted with Et2O. After drying on MgSO4 and concentration under vacuum, the crude product is purified by column chromatography (eluent CH2Cl2/MeOH 9:1) to give 1.39 g of the expected compound in the form of a light yellow oil (yield: 52%). 163

[0739] C11H13N3

[0740] M=187.27 g/mol

[0741] 1 H NMR base (CDCl3) δ (ppm): 7.38-7.28 (m, 1H, ArH); 7.17-7.04 (m, 3H, ArH); 3.24-3.12 (m, 4H, CH2(3), CH2(5)); 3.09-2.97 (m, 4H, CH2(2), CH2(6)).

[0742] 2-[4-(3-benzonitrile)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 119

[0743] The reaction between 2.37 g (8.25 mmol) of 2-bromoethyl-N-(2-ethoxyphenyl)carbamate, 1.4 ml (8.25 mmol, 1 eq) of DIEA and 1.4 g (7.42 mmol, 0.9 eq) of 1-(3-benzonitrile)phenylpiperazine and a few KI crystals in 15 ml of DMF, followed by treatment (medium taken up in AcOEt washed with saturated NaCl solution), gives an oil which is purified by column chromatography (eluent AcOEt/petroleum ether 8:2) to produce a mixture which is again purified by column chromatography (eluent CH2Cl2 then CH2Cl2/MeOH 9:1). The product is a colorless oil stored as the hydrochloride. 320 mg of a white powder (yield: 10%) is obtained. 164

[0744] C22H26N4O3.HCl

[0745] M=430.94 g/mol

[0746] 1 H NMR base (CDCl3) δ (ppm): 8.10 (dd, Jo=6 Hz, Jm=3 Hz, 1H, CH(6)); 7.41-7.22; 7.18-6.76 (m, 8H, NH, ArH); 4.36 (t, J=6 Hz, 2H, OCH2); 4.10 (q, J=7 Hz, 2H, OCH2); 3.33-3.19 (m, 4H, CH2(3′), CH2(5′)); 2.78 (t, J=6 Hz, 2H, CH2N); 2.77-2.64 (m, 4H, CH2(2′), CH2(6′)); 1.45 (t, J=7 Hz, 2H, CH3).

[0747] 13 C NMR (CD3OD) δ (ppm): 154.6 (CO2); 151.4 (C1″); 150.9 (C2); 131.5 (C5′); 128.2 (C1); 125.4 (C4″); 125.3 (1C, C2″ . . . C6″); 125.1 (1C, C2″ . . . C6″); 122.1 (C4); 121.6 (C6); 120.3 (C5); 119.9 (CN); 114.2 (C3″); 112.8 (C3); 65.4 (CH2O); 59.6 (CH2O); 57.0 (CH2N); 53.2 (2C, C2′, C6′); 46.8 (2C, C3′, C5′); 15.1 (CH3).

Elemental analysis:
		Calc. (1 H2O)	Exp.
	% C	58.85	58.79
	% H	6.51	6.53
	% N	12.47	12.12

[0748] 1-(3-nitrophenyl)piperazine

[0749] 28.4 g (0.33 mol, 5.5 eq) of piperazine is dissolved in 50 ml of DMSO. 6.4 ml (60 mmol) of 2-fluoronitrobenzene is added. The mixture is heated at 100° C. for 60 hours. After cooling, the reaction mixture is transferred into 530 ml of water. The precipitate formed is filtered and the filtrate extracted with Et2O. After drying on MgSO4 and concentration under vacuum, the crude product is purified by column chromatography (eluent CH2Cl2/MeOH 9:1) to produce 8.04 g of the expected compound in the form of an orange oil which crystallizes at room temperature (yield: 65%). 165

[0750] C10H13N3O2

[0751] M=207.26 g/mol

[0752] 1 H NMR (CDCl3) δ (ppm): 7.70 (t, Jm=2 Hz, 1H, CH(2′)); 7.64 (ddd, Jo=8 Hz, Jm=2 Hz, Jm=0.6 Hz, 1H, CH(4′)); 7.36 (t, Jo=8 Hz, 1H, CH(5′)), 7.17 (ddd, Jo=8 Hz, Jm=2 Hz, Jm=0.6 Hz, 1H, CH(6′)); 3.30-3.18 (m, 4H, CH2(3), CH2(5)); 3.09-2.97 (m, 4H, CH2(2), CH2(6)),

[0753] 2-[4-(3-nitrophenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 120

[0754] The reaction between 0.47 g (1.62 mmol) of 2-bromoethyl-N-(2-ethoxyphenyl)carbamate 28, 282 μl (1.62 mmol, 1 eq) of DIEA and 0.42 g (1.46 mmol, 0.9 eq) of 1-(3-nitro)phenylpiperazine and a few KI crystals in 5 ml of DMF, followed by treatment, gives an oil which is purified by column chromatography (eluent AcOEt/petroleum ether 8:2) to produce a mixture which is again purified by column chromatography (eluent CH2Cl2 then CH2Cl2/MeOH 9:1). The product is an orange oil stored as the hydrochloride. One obtains 384 mg of an orange solid (yield: 58%). 166

[0755] C21H26N4O5.HCl

[0756] M=450.93 g/mol

[0757] 1 H NMR base (CDCl3) δ (ppm): 8.10 (dd, Jo=6 Hz, Jo=3 Hz, 1H, CH(6)); 7.70 (t, Jm=20 Hz, 1H, CH(2″)); 7.64 (ddd, Jo=8.2 Hz, Jo=2 Hz, Jm=0.8 Hz, 1H, CH(4″)); 7.36 (t, Jo=8.2 Hz, 1H, CH(5″)), 7.30 (sl, 1H, NH); 7.17 (ddd, Jm=8.2 Hz, Jm=2 Hz, Jm=0.8 Hz, 1H, CH(6″)); 7.03-6.80 (m, 3H, CH(3), CH(4), CH(5)); 4.35 (t, J=6 Hz, 2H, OCH2); 4.09 (q, J=7 Hz, 2H, OCH2); 3.56-3.26 (m, 4H, CH2(3′), CH2(5′)); 2.77 (t, J=6 Hz, 2H, CH2N); 2.75-2.64 (m, 4H, CH2(2′), CH2(6′)); 1.45 (t, J=7 Hz, 2H, CH3).

[0758] 13 C NMR base (CDCl3) 6 (ppm): 153.2 (CO2); 151.7 (C1″); 149.2 (C3″); 146.8 (C2); 129.5 (C5s); 127.5 (CI); 122.7 (2C, C4, C4″); 120.9 (C6); 118.2 (C5); 113.5 (C6″); 110.8 (C3); 109.5 (C2″); 64.0 (CH2O); 61.9 (CH2O); 56.9 (CH2N); 52.9 (2C, C2′, C6′); 48.2 (2C, C3′, C5s); 14.8 (CH3).

Elemental analysis:
		Calc. (3/4 H2O)	Exp.
	% C	54.30	54.31
	% H	6.18	6.30
	% N	12.06	11.81

[0759] 2-[4-(3-aminophenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 121 1.08 g (2.60 mmol) of nitro derivative is dissolved in 40 ml of methanol and a spatula tip of Raney nickel is added, placed in a hydrogen atmosphere and shaken at room temperature. The progress of the reaction is monitored by TLC (mobile phase AcOEt/petroleum ether 8:2). After 30 min the reaction mixture becomes decolored. The mixture is then filtered on celite and the filtrate concentrated under reduced pressure. The crude product is taken up in ether, extracted with 1 M HCl. The extracted aqueous phase is then adjusted to alkaline pH by addition of K2CO3, then extracted with AcOEt. After drying on Na2SO4 and evaporation of the organic phase, 0.8 g of a pure yellow solid is obtained (yield: 80%). 167

[0760] C21H28N4O3

[0761] M=384.53 g/mol

[0762] 1 H NMR (CDCl3) δ (ppm): 8.09 (dd, Jo=6 Hz, Jm=3 Hz, 1H, CH(6)); 7.31 (sl, 1H, NH); 7.10-6.79 (m, 4H, CH(3), CH(4), CH(5), ArH); 6.39-6.17 (m, 3H, ArH); 4.35 (t, J=6 Hz, 2H, OCH2); 4.09 (q, J=7 Hz, 2H, OCH2); 3.59 (sl, 2H, NH2); 3.25-3.14 (m, 4H, CH2(3′), CH2(5′)); 2.76 (t, J=6 Hz, 2H, CH2N); 2.70-2.60 (m, 4H, CH2(2′), CH2(6′)); 1.45 (t, J=7 Hz, 2H, CH3).

[0763] 13 C NMR (CDCl3) δ (ppm): 153.4 (CO2); 152.5 (C1″); 147.3 (C3″); 146.9 (C2); 129.9 (C5″); 127.7 (C1); 122.7 (C4); 120.9 (C6); 118.3 (C5); 110.9 (C3); 107.0 (C4″); 106.9 (C6″); 102.9 (C2″); 64.0 (CH2O); 62.0 (CH2O); 57.0 (CH2N); 53.4 (2C, C2′, C6′); 48.8 (2C, C3, C5s); 14.8 (CH3).

Elemental analysis:
	Calc. (1/4 H2O)	Exp.
	% C	64.84	64.93
	% H	7.38	7.37
	% N	14.40	14.23

[0764] 2-{4-[3-(acetylamino)phenyl]piperazino}ethyl-N-(2-ethoxyphenyl)carbamate 122

[0765] To a mixture of 77 mg (0.2 mmol) of 2-[4-(3-aminophenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate derivative and 44.6 μl (0.32 mmol, 1.6 eq) of TEA in 5 ml of THF, 71 μl (0.3 mmol, 1.5 eq) of acetyl chloride is added. Shaking is carried out at room temperature for about 20 hours. Then H2O is added and the aqueous phase is extracted with CH2Cl2. After drying on Na2SO4 and concentration under vacuum, a white solid is obtained which is purified by column chromatography (eluent CH2Cl2/iPrOH 9:1) to produce 36 mg of the expected product as a brown oil (yield: 43%). 168

[0766] C23H30N4O4

[0767] M=426.51 g/mol

[0768] 1 H NMR (CDCl3) δ (ppm): 8.08 (dd, Jo=6 Hz, Jm=3 Hz, 1H, CH(6)); 7.41 (sl, 1H, NH); 7.31 (sl, 1H, NH); 7.16 (t, J=8., 2 Hz, 1H, ArH); 7.04-6.79 (m, 5H, ArH); 6.72-6.59 (m, 1H, ArH); 4.34 (t, J=6 Hz, 2H, OCH2); 4.08 (q, J=7 Hz, 2H, OCH2); 3.30-3.10 (m, 4H, CH2(3′), CH2(5′)); 2.75 (t, J=6 Hz, 2H, CH2N); 2.70-2.59 (m, 4H, CH2(2′), CH2(6′)); 2.13 (s, 3H, CH3); 1.45 (t, J=7 Hz, 2H, CH3).

[0769] 13 C NMR (CDCl3) δ (ppm): 162.8 (CO); 153.3 (CO2); 151.8 (C1″); 146.9 (C2); 138.8 (C3″); 129.3 (C5″); 127.5 (C1); 122.7 (C4); 120.8 (C6); 118.2 (C5); 111.7 (2C, C4″, C6″); 110.8 (C3); 107.5 (C2″); 64.0 (CH2O); 61.9 (CH2O); 56.9 (CH2N); 53.2 (2C, C2′, C6′); 48.6 (2C, C3′, C5′); 24.5 (CH3); 14.8 (CH3).

[0770] HPLC: t=1.51 min

[0771] MS: 427.55 (MH+)

[0772] HPLC purity: 100%

[0773] 2-{4-[3-(methylsulfonylaniino)phenyl] piperazino}ethyl-N-(2-ethoxyphenyl)carbamate 123

[0774] To a mixture of 77 mg (0.2 mmol) of 2-[4-(3-aminophenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate derivative and 56 μl (0.4 mmol, 2 eq) of TEA in 5 ml of THF, 31 μl (0.4 mmol, 2 eq) of mesyl chloride is added. Shaking is carried out at room temperature for about 20 hours. After concentration under reduced pressure, H2O is added and the aqueous phase is extracted with CH2Cl2. After drying on Na2SO4 and concentration of the organic phase, a brown oil is obtained which is purified by column chromatography (eluent AcOEt/petroleum ether 8:2) to produce 61 mg of the expected product as a brown oil (yield: 66%). 169

[0775] C22H30N4SO5

[0776] M=462.63 g/mol

[0777] 1 H NMR (CDCl3) δ (ppm): 8.08 (dd, Jo=6 Hz, Jm=3 Hz, 1H, CH(6)); 7.31 (sl, 1H, NH); 7.20 (t, J=8, 2 Hz, 1H, ArH); 7.06-6.58 (m, 6H, ArH); 6.50 (sl, 1H, NH); 4.35 (t, J=6 Hz, 2H, OCH2); 4.09 (q, J=7 Hz, 2H, OCH2); 3.34-3.17 (m, 4H, CH2(3′), CH2(5′)); 2.99 (s, 3H, CH3); 2.78 (t, 2H CH2N); 2.70-2.60 (m, 4H, CH2(2′), CH2(6′)); 1.45 (t, J=7 Hz, 2H, CH3).

[0778] 13 C NMR (CDCl3) δ (ppm): 156.8 (CO2); 152.3 (C1″); 146.9 (C2); 137.8 (C3″); 130.1 (C5′); 127.6 (C1); 122.7 (C4); 120.9 (C6); 118.3 (C5); 112.8 (IC, C4-C6″); 111.3 (1C, C4′-C6′); 110.9 (C3); 107.8 (C2′); 64.1 (CH2O); 62.0 (CH2O); 57.0 (CH2N); 53.2 (2C, C2, C6′); 48.5 (2C, C3′, C5′); 39.1 (CH3); 14.7 (CH3).

[0779] HPLC: t=1.55 min

[0780] MS: 463.49 (MH+)

[0781] HPLC purity: 93%

[0782] 2-[4-(3-{[(ethylamino)carbonyl]iamino}phenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 124

[0783] Dilute 120 μl (1.5 mmol, 3 éeq) of ethylisocyanate in 5 ml of CH2Cl2. Add 192 mg of 2-[4-(3-aminopheenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate dissolved in 5 ml of CH2Cl2 and react under a reflux condenser for 18 hours, then at room temperature for approximately 60 hours. The reaction medium is then concentrated under reduced pressure, taken up in 4 ml of CH2Cl2 and reacted with methylisocyanate resin (0.5 mmol, 1 eq) at room temperature for 24 hours. After filtration of the resin, the filtrate is evaporated to give a yellow oil which crystallizes at room temperature. One obtains 149 mg of the expected pure compound (yield: 65%). 170

[0784] C24H33N5O4

[0785] M=455.62 g/mol

[0786] 1 H NMR (CDCl3) δ (ppm): 8.07 (dd, Jo=6 Hz, Jm=3 Hz, 1H, CH(6)); 7.48 (sl, 1H, NH); 7.36 (sl, 1H, NH); 7.30-6.42 (m, 7H, ArH); 5.60 (t, J=5, 3 Hz, 1H, NH); 4.33 (t, J=6 Hz, 2H, OCH2); 4.08 (q, J=7 Hz, 2H, OCH2); 3.33-3.06 (m, 6H, CH2(3′), CH2(5′), CH2); 2.75 (t, 2H, J=6 Hz CH2N); 2.70-2.54 (m, 4H, CH2(2′), CH2(6′)); 1.44 (t, J=7 Hz, 2H, CH3); 1.09 (t, J=7.2 Hz, 2H, CH3).

[0787] 13 C NMR (CDCl3) δ (ppm): 156.6 (CO); 153.3 (CO2); 151.6 (C1″); 147.0 (C2); 140.3 (C3″); 129.3 (C5″); 127.4 (C1); 122.9 (C4); 120.8 (C6); 118.3 (C5); 110.9 (2C, C4, C6″); 110.0 (C3); 107.2 (C2″); 64.0 (CH2O); 61.7 (CH2O); 56.8 (CH2N); 53.1 (2C, C2′, C6′); 48.4 (2C, C3′, C5s); 34.7 (CH2); 15.3 (CH3); 14.7 (CH3).

Claims

1. Compound represented by the general formula (I):

2. Compound represented by the general formula (I) according to claim 1, wherein R1 represents a methyl or ethyl group.

3. Compound represented by the general formula (I) according to any one of claims 1 or 2, wherein R3 represents a heterocycle having 6 atoms, chosen from among pyridyl, pyrimidinyl, pyridazinyl, pirazinyl, such group possibly being substituted by one or more groups chosen from among halogeno, alkoxy, cyano, hydroxy, nitro, alkyl, halogenoalkyl, alkylthio, —NHR8, —NHCOR8, —NHSO2R8, —NHCONR8R9.

4. Compound represented by the general formula (I) according to any one of claims 1 or 2, wherein R3 represents a phenyl group possibly substituted.

5. Compound represented by the general formula (I) according to claim 1, wherein R1 represents a methyl or ethyl group and R3 represents a phenyl group possibly substituted.

6. Compound chosen from among: 2-[4-(3-methoxyphenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 2-[4-(3-methylphenyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 2-[4-(2-pyridinyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 2-[4-(3-pyridazinyl)piperazino]ethyl-N-(2-ethoxyphenyl)carbamate 3-[4-(4-fluorophenyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 3-[4-(2-pyridinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 3-[4-(4-pyridinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 3-[4-(3-pyridazinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 3-[4-(4-pyrimidinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 3-[4-(2-pyrimidinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 3-[4-(6-methyl-3-pyridazinyl)piperazino]propyl-N-(2-ethoxyphenyl)carbamate 2-(4-phenylpiperazino)ethyl-N-(2-methoxyphenyl)carbamate 2-[4-(3-methoxyphenyl)piperazino]ethyl-N-(2-meethoxyphenyl)carbamate 2-[4-(2-pyridinyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 2-[4-(3-pyridazinyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 2-[4-(2-pyrimidinyl)piperazino]ethyl-N-(2-methoxyphenyl)carbamate 3-[4-(4-pyridinyl)piperazino]propyl-N-(2-methoxypheenyl)carbamate and the salts thereof.

7. Pharmaceutical composition comprising a compound according to any one of claims 1 to 6.

8. Pharmaceutical composition according to claim 7, for the treatment or prophylaxis of diseases involving the 5-HT4 receptor.

9. Pharmaceutical composition according to claim 7, for the treatment or prophylaxis of gastrointestinal disorders, central nervous system disorders, cardiac disorders or urologic disorders.

10. Use of a compound according to any one of claims 1 to 6 for preparing a pharmaceutical composition for implementing a method of treatment or prophylaxis in the human or animal body.

11. Use of a compound according to any one of claims 1 to 6 for preparing a pharmaceutical composition for the therapeutic or preventive treatment of gastrointestinal disorders, central nervous system disorders, cardiac disorders or urologic disorders.

12. Method for preparing a compound according to any one of claims 1 to 6, wherein a product represented by formula (II) is reacted with a product represented by formula (III):

13. Method for preparing a compound according to any one of claims 1 to 6, wherein a product represented by formula (XIV) is reacted with a product represented by formula (XII):

14. Method according to claim 13, wherein the spacer group K has the following formula (XV):

15. Method according to claim 13 or 14, wherein it comprises preparing a compound represented by formula (XIV) according to the reaction diagram shown in FIG. 5.

16. Method for preparing a compound according to any one of claims 1 to 6, wherein a product represented by formula (II) is reacted with a product represented by formula (III) in which the groups R1, R2, R3, R4, R6, Y, J, G and n are defined as in claim 1, in a CXCl2/pyridine system, and the resulting product is recovered.

17. Method for preparing a compound according to any one of claims 1 to 6, wherein a product represented by formula (II) is reacted with a product represented by formula (XVI) and a product represented by formula (XVII), in which the groups R1, R2, R3, R4, R6, Y, J, G and n are defined as in claim 1, and the resulting product is recovered.

18. Method for preparing a compound according to any one of claims 1 to 6, wherein a product represented by formula (II) is reacted with a product represented by formula (XVIII) in which the groups R1, R2, R3, R4, R6, Y, J, G and n are defined as in claim 1, and the resulting product is recovered.