Patent Application
20210309658
The present invention relates to organic compounds useful for therapy and/or prophylaxis in a mammal, and in particular to inhibitors of DNA gyrase and/or topoisomerase IV useful for treatment and/or prophylaxis of bacterial infection.
Bacterial infections pose a continuing medical problem because anti-bacterial drugs eventually engender resistance in the bacteria on which they are used. Bacterial resistance against virtually all current antibiotic drugs are increasing. Many forms of antibiotic resistance can even cross international boundaries and spread with remarkable speed. Thus novel classes of antibacterial compounds are urgently needed.
One target for development of anti-bacterial drugs has been DNA gyrase and topoisomerase IV (bacterial type IIA topoisomerases), which are essential to cell life, that solve DNA topological problems resulting from the replication, transcription, and recombination of DNA. DNA Gyrase controls DNA supercoiling and relieves topological stress that occurs when the DNA strands are untwisted such as during replication. Topoisomerase IV primarily resolves linked chromosome dimers at the conclusion of DNA replication. Both enzymes can introduce double stranded DNA breaks; pass a second DNA strand through the break and rejoining the broken strands. The activity of both enzymes is driven by the binding and hydrolysis of ATP. Bacterial DNA gyrase consists of two A (GyrA) and two B (GyrB) subunits. Binding and cleavage of the DNA is associated with GyrA, whereas ATP is bound and hydrolyzed by GyrB. Bacterial Topoisomerase IV is also a hetero-tetramer that consists of two C (ParC) and two E (ParE) subunits. The latter subunits bind ATP like GyrB in order to supply energy necessary for catalytic turnover of the enzymes.
Inhibition of DNA gyrase and topoisomerase IV has potential for the development of broad-spectrum antibiotics. The enzymes are highly conserved across a broad range of gram-positive and gram-negative pathogens. There are two classes of antibiotics that demonstrated such mechanism of action. The first, well-represented by the quinolones, inhibits GyrA and ParC subunits by stabilizing the cleaved DNA-enzyme complex, thus inhibiting overall gyrase function, leading to cell death. Novobiocin, the only marketed drug in the second class, exerts its effect by blocking the ATPase activity of the enzymes. Novobiocin was identified in 1950s. But its use declined rapidly and it was eventually withdrawn from the market, mainly due to its low permeability in many bacteria strains, rise of spontaneous resistance development, and the development of more effective drugs, such as penicillinase-stable penicillins and the first cephalosporins in 1960s and 1970s.
Recently, strong inhibition of DNA gyrase and/or topoisomerase IV has been recognized to be important for low resistance development in bacterial strains treated by inhibitors of the enzymes. Inhibitors of bacterial DNA gyrase and/or topoisomerase IV with different mechanism of action compare to the widely used quinolones will exhibit minimal cross resistance, and will be potentially useful in combating quinolone resistance that has increased significantly in the past few years.
The present invention relates to novel compounds of formula (I),
Objects of the present invention are novel compounds of formula (I), their manufacture, medicaments based on a compound in accordance with the invention and their production as well as the use of compounds of formula (I) for the treatment or prophylaxis of bacterial infection. The use of compounds of formula (I) as DNA gyrase and/or topoisomerase IV inhibitors is also one of the objections of present invention. The compounds of formula (I) showed superior anti-bacterial activity, good solubility, good CC50 profiles, improved microsomal stability and/or improved PK profile.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Furthermore, the following definitions are set forth to illustrate and define the meaning and scope of the various terms used to describe the invention.
The term “C1-6alkyl” denotes a saturated, linear or branched chain alkyl group containing 1 to 6, particularly 1 to 4 carbon atoms, for example methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, tert-butyl and the like. Particular “C1-6alkyl” groups are methyl, ethyl and propyl.
The term “C1-6alkoxy” denotes a group of the formula C1-6alkyl-O—. Examples of C1-6alkoxy group include, but not limited to, methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, isobutoxy and tert-butoxy. Particular “C1-6alkoxy” groups are methoxy, ethoxy and isopropoxy.
The term “C3-7cycloalkyl” denotes to a saturated carbon ring containing from 3 to 7 carbon atoms, particularly from 3 to 6 carbon atoms, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and the like. Particular “C3-7cycloalkyl” group is cyclopropyl.
The term “halogen” and “halo” are used interchangeably herein and denote fluoro, chloro, bromo, or iodo.
The term “haloC1-6alkyl” denotes an alkyl group wherein at least one of the hydrogen atoms of the alkyl group has been replaced by same or different halogen atoms, particularly fluoro atom. Examples of haloC1-6alkyl include monofluoro-, difluoro- or trifluoro-methyl, -ethyl or -propyl, for example 3,3,3-trifluoropropyl, 2-fluoroethyl, 2,2,2-trifluoroethyl, fluoromethyl, difluoromethyl, trifluoromethyl and trifluoroethyl.
The term “azetidinyloxy” denotes azetidinyl-O—.
The term “piperidinyloxy” denotes piperidinyl-O—.
The term “pyrrolidinyloxy” denotes pyrrolidinyl-O—.
The term “pharmaceutically acceptable salts” denotes salts which are not biologically or otherwise undesirable. Pharmaceutically acceptable salts include both acid and base addition salts.
The term “pharmaceutically acceptable acid addition salt” denotes those pharmaceutically acceptable salts formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, carbonic acid, phosphoric acid, and organic acids selected from aliphatic, cycloaliphatic, aromatic, araliphatic, heterocyclic, carboxylic, and sulfonic classes of organic acids such as formic acid, acetic acid, propionic acid, glycolic acid, gluconic acid, lactic acid, pyruvic acid, oxalic acid, malic acid, maleic acid, maloneic acid, succinic acid, fumaric acid, tartaric acid, citric acid, aspartic acid, ascorbic acid, glutamic acid, anthranilic acid, benzoic acid, cinnamic acid, mandelic acid, embonic acid, phenylacetic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, and salicyclic acid.
The term “pharmaceutically acceptable base addition salt” denotes those pharmaceutically acceptable salts formed with an organic or inorganic base. Examples of acceptable inorganic bases include sodium, potassium, ammonium, calcium, magnesium, iron, zinc, copper, manganese, and aluminum salts. Salts derived from pharmaceutically acceptable organic nontoxic bases includes salts of primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins, such as isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, ethanolamine, 2-diethylaminoethanol, trimethamine, dicyclohexylamine, lysine, arginine, histidine, caffeine, procaine, hydrabamine, choline, betaine, ethylenediamine, glucosamine, methylglucamine, theobromine, purines, piperizine, piperidine, N-ethylpiperidine, and polyamine resins.
The term “therapeutically effective amount” denotes an amount of a compound or molecule of the present invention that, when administered to a subject, (i) treats or prevents the particular disease, condition or disorder, (ii) attenuates, ameliorates or eliminates one or more symptoms of the particular disease, condition, or disorder, or (iii) prevents or delays the onset of one or more symptoms of the particular disease, condition or disorder described herein. The therapeutically effective amount will vary depending on the compound, the disease state being treated, the severity of the disease treated, the age and relative health of the subject, the route and form of administration, the judgement of the attending medical or veterinary practitioner, and other factors.
The term “pharmaceutical composition” denotes a mixture or solution comprising a therapeutically effective amount of an active pharmaceutical ingredient together with pharmaceutically acceptable excipients to be administered to a mammal, e.g., a human in need thereof.
Inhibitors of DNA Gyrase and/or Topoisomerase IV
The present invention relates to a compound of formula (I),
A further embodiment of present invention is (ii) a compound of formula (I) according to (i), wherein
A further embodiment of present invention is (iii) a compound of formula (I) according to (i) or (ii), or pharmaceutically acceptable salt thereof, wherein
A further embodiment of present invention is (iv) a compound of formula (I) according to any one of (i) to (iii), or pharmaceutically acceptable salt thereof, wherein R2 is pyrazolyl which is substituted once or twice by substituents independently selected from haloC1-6alkyl, aminoC1-6alkyl, aminocarbonyl, C1-6alkyl, cyano, and hydroxyC1-6alkyl.
A further embodiment of present invention is (v) a compound of formula (I) according to any one of (i) to (iv), wherein
or pharmaceutically acceptable salt thereof.
A further embodiment of present invention is (vi) a compound of formula (I) according to any one of (i) to (v), wherein
or pharmaceutically acceptable salt thereof.
Another embodiment of present invention is that (vii) compounds of formula (I) are selected from:
X1, X2, X3 and X4 are halogen.
Compound of formula (I) can be prepared according to Scheme 1. Nucleophilic substitution of ortho-fluoro nitrobenzene (Ia) with amine R6—NH2 affords aniline (Ib). The aniline (Ib) can be protected with di-tert-butyl carbonate to give the protected aniline (Ic). The nitro group in aniline (Ic) can be reduced by a reducing agent, such as H2 with palladium catalysts, to give the compound of formula (Id). Coupling of the compound of formula (Id) with tri-halogenated pyridine can be achieved using palladium catalysts and phosphine ligands to give compound of formula (Ie). Cyclization of compound of formula (Ie) using palladium catalysts and phosphine ligands gives compound of formula (If). Compound of formula (Ig) can be obtained from formula (If) through oxidation of the pyridine followed by halogenation, such as treatment of POCl3 or POBr3. Coupling of compound of formula (Ig) to introduce R2 can be achieved either through Buchwald-Hartwig Cross Coupling Reaction for certain C—N bond formation (with R2 bearing a basic N), or a palladium catalyzed Suzuki coupling for C—C bond formation (with R2 as a boronic ester or boronic acid), to give compound of formula (Ih). Further coupling of compound of formula (Ih) to introduce R1 can be achieved using a palladium catalyzed Suzuki coupling to give compound of formula (Ii). Deprotection of compound of formula (Ii) in the presence of an acid, such as trifluoroacetic acid, affords compound of formula (I).
This invention also relates to a process for the preparation of a compound of formula (I) comprising the reaction of compound of formula (Ii),
with an acid, which can be for example trifluoroacetic acid;
wherein R1 to R6 are defined above. A compound of formula (I) when manufactured according to the above process is also an object of the invention.
Another embodiment provides pharmaceutical compositions or medicaments containing the compounds of the invention and a therapeutically inert carrier, diluent or excipient, as well as methods of using the compounds of the invention to prepare such compositions and medicaments. In one example, compounds of formula (I) may be formulated by mixing at ambient temperature at the appropriate pH, and at the desired degree of purity, with physiologically acceptable carriers, i.e., carriers that are non-toxic to recipients at the dosages and concentrations employed into a galenical administration form. The pH of the formulation depends mainly on the particular use and the concentration of compound, but preferably ranges anywhere from about 3 to about 8. In one example, a compound of formula (I) is formulated in an acetate buffer, at pH 5. In another embodiment, the compounds of formula (I) are sterile. The compound may be stored, for example, as a solid or amorphous composition, as a lyophilized formulation or as an aqueous solution.
Compositions are formulated, dosed, and administered in a fashion consistent with good medical practice. Factors for consideration in this context include the particular disorder being treated, the particular mammal being treated, the clinical condition of the individual patient, the cause of the disorder, the site of delivery of the agent, the method of administration, the scheduling of administration, and other factors known to medical practitioners. The “effective amount” of the compound to be administered will be governed by such considerations, and is the minimum amount necessary to reduced bacterial load or improve host survival through the inhibition of bacterial DNA gyrase and/or Topoisomerase IV. For example, such amount may be below the amount that is toxic to normal cells, or the mammal as a whole.
In one example, the pharmaceutically effective amount of the compound of the invention administered parenterally per dose will be in the range of about 0.1 to 1000 mg/kg, alternatively about 1 to 100 mg/kg of patient body weight per day. In another embodiment, oral unit dosage forms, such as tablets and capsules, preferably contain from about 5 to about 5000 mg of the compound of the invention.
The compounds of the invention may be administered by any suitable means, including oral, topical (including buccal and sublingual), rectal, vaginal, transdermal, parenteral, subcutaneous, intraperitoneal, intrapulmonary, intradermal, intrathecal and epidural and intranasal, and, if desired for local treatment, intralesional administration. Parenteral infusions include intramuscular, intravenous, intraarterial, intraperitoneal, or subcutaneous administration.
The compounds of the present invention may be administered in any convenient administrative form, e.g., tablets, powders, capsules, solutions, dispersions, suspensions, syrups, sprays, suppositories, gels, emulsions, patches, etc. Such compositions may contain components conventional in pharmaceutical preparations, e.g., diluents, carriers, pH modifiers, sweeteners, bulking agents, and further active agents.
A typical formulation is prepared by mixing a compound of the present invention and a carrier or excipient. Suitable carriers and excipients are well known to those skilled in the art and are described in detail in, e.g., Ansel, Howard C., et al., Ansel's Pharmaceutical Dosage Forms and Drug Delivery Systems. Philadelphia: Lippincott, Williams & Wilkins, 2004; Gennaro, Alfonso R., et al. Remington: The Science and Practice of Pharmacy. Philadelphia: Lippincott, Williams & Wilkins, 2000; and Rowe, Raymond C. Handbook of Pharmaceutical Excipients. Chicago, Pharmaceutical Press, 2005. The formulations may also include one or more buffers, stabilizing agents, surfactants, wetting agents, lubricating agents, emulsifiers, suspending agents, preservatives, antioxidants, opaquing agents, glidants, processing aids, colorants, sweeteners, perfuming agents, flavoring agents, diluents and other known additives to provide an elegant presentation of the drug (i.e., a compound of the present invention or pharmaceutical composition thereof) or aid in the manufacturing of the pharmaceutical product (i.e., medicament).
An example of a suitable oral dosage form is a tablet containing about 10 to 500 mg of the compound of the invention compounded with about 40 to 400 mg anhydrous lactose, about 5 to 50 mg sodium croscarmellose, about 5 to 50 mg polyvinylpyrrolidone (PVP) K30, and about 1 to 10 mg magnesium stearate. The powdered ingredients are first mixed together and then mixed with a solution of the PVP. The resulting composition can be dried, granulated, mixed with the magnesium stearate and compressed to tablet form using conventional equipment. An example of an aerosol formulation can be prepared by dissolving the compound, for example 5 to 1000 mg) of the invention in a suitable buffer solution, e.g. a phosphate buffer, adding a tonicifier, e.g. a salt such sodium chloride, if desired. The solution may be filtered, e.g., using a 0.2 micron filter, to remove impurities and contaminants.
An embodiment, therefore, includes a pharmaceutical composition comprising a compound of formula (I), or a stereoisomer or pharmaceutically acceptable salt thereof. In a further embodiment includes a pharmaceutical composition comprising a compound of formula (I), or a stereoisomer or pharmaceutically acceptable salt thereof, together with a pharmaceutically acceptable carrier or excipient.
Another embodiment includes a pharmaceutical composition comprising a compound of formula (I) for use in the treatment and/or prophylaxis of bacterial infections.
In some embodiments, the compounds of this invention may be administered, as part of a single or multiple dosage regimen, orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally, or via an implanted reservoir. The term parenteral as used includes subcutaneous, intracutaneous, intravenous, intramuscular, intra-articular, intrasynovial, intrasternal, intrathecal, intralesional and intracranial injection or infusion techniques. Typically, the pharmaceutical compositions of the invention will be administered from about 1 to 5 times per day or alternatively, as a continuous infusion upon improvement of a patient's condition.
The compounds of the invention are useful for treatment and/or prophylaxis of bacterial infection in humans or other animals by administering to the subject in need of a therapeutically effective amount of compound of formula (I), or a pharmaceutically acceptable salt, or enantiomer or diastereomer thereof. The compounds and methods of the invention are particularly well suited for human patients infected by pathogens that include Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa. Examples of bacterial organisms that may also be controlled by the compounds of the invention include, but not limited to, the following Gram-Positive and Gram-Negative organisms: Streptococcus pneumoniae. Streptococcus pyogenes, Enterococcus faecalis, Enterococcus faecium, Enterobacter spp. species, Proteus spp. species, Serratia marcescens, Staphylococcus aureus, Coag. Neg. Staphylococci, Haemophilus influenzae, Bacillus anthraces, Mycoplasma pneumoniae, Moraxella catarrhalis, Chlamydophila pneumoniae. Chlamydia trachomatis, Legionella pneumophila, Mycobacterium tuberculosis, Helicobacter pylori, Staphylococcus saprophyticus. Staphylococcus epidermidis, Francisella tularensis, Yersinia pestis, Clostridium difficile, Bacteroides spp. species Neisseria gonorrhoeae, Neisseria meningitidis, Burkholderia pseudomallei, Burkholderia mallei, Borrelia burgdorferi, Mycobacterium avium complex, Mycobacterium abscessus, Mycobacterium kansasii and Mycobacterium ulcerans.
Examples of bacterial infections may include, but not limited to, upper respiratory infections, lower respiratory infections, ear infections, pleuropulmonary and bronchial infections, complicated urinary tract infections, uncomplicated urinary tract infections, intra-abdominal infections, cardiovascular infections, a blood stream infection, sepsis, bacteremia, CNS infections, skin and soft tissue infections, GI infections, bone and joint infections, genital infections, eye infections, or granulomatous infections. Examples of specific bacterial infections include, but not limited to, uncomplicated skin and skin structure infections (uSSSI), complicated skin and skin structure infections (cSSSI), catheter infections, pharyngitis, sinusitis, otitis externa, otitis media, bronchitis, empyema, pneumonia, community-acquired bacterial pneumoniae (CABP), hospital-acquired pneumonia (HAP), hospital-acquired bacterial pneumonia, ventilator-associated pneumonia (VAP), diabetic foot infections, vancomycin resistant enterococci infections, cystitis and pyelonephritis, renal calculi, prostatitis, peritonitis, complicated intra-abdominal infections (cIAI) and other inter-abdominal infections, dialysis-associated peritonitis, visceral abscesses, endocarditis, myocarditis, pericarditis, transfusion-associated sepsis, meningitis, encephalitis, brain abscess, osteomyelitis, arthritis, genital ulcers, urethritis, vaginitis, cervicitis, gingivitis, conjunctivitis, keratitis, endophthalmitisa, an infection in cystic fibrosis patients or an infection of febrile neutropenic patients.
Furthermore, the invention relates to the use of a compound of formula (I) for the treatment and/or prophylaxis of bacterial infection. The invention relates to the use of a compound of formula (I) for the preparation of a medicament for the treatment and/or prophylaxis of bacterial infection. Another embodiment includes a method for the treatment or prophylaxis of bacterial infection which method comprises administering an effective amount of a compound of formula (I), or pharmaceutically acceptable salt, or enantiomer or diastereomer thereof.
The invention will be more fully understood by reference to the following examples. They should not, however, be construed as limiting the scope of the invention.
Abbreviations used herein are as follows:
Intermediates and final compounds were purified by flash chromatography using one of the following instruments: i) Biotage SP1 system and the Quad 12/25 Cartridge module. ii) ISCO combi-flash chromatography instrument. Silica gel Brand and pore size: i) KP-SIL 60 Å, particle size: 40-60 μm; ii) CAS registry NO: Silica Gel: 63231-67-4, particle size: 47-60 micron silica gel; iii) ZCX from Qingdao Haiyang Chemical Co., Ltd, pore: 200-300 or 300-400.
Intermediates and final compounds were purified by preparative HPLC on reversed phase column using X Bridge™ Perp C18 (5 μm, OBD™ 30×100 mm) column or SunFire™ Perp C18 (5 μm, OBD™ 30×100 mm) column.
Chiral Separation was conducted on Thar 350 preparative SFC using ChiralPak AD-10μ (200×50 mm I.D.) with mobile phase A for CO2 and B for ethanol.LC/MS spectra were obtained using a Waters UPLC-SQD Mass. Standard LC/MS conditions were as follows (running time: 3 minutes):
Acidic condition: A: 0.1% formic acid and 1% acetonitrile in H2O; B: 0.1% formic acid in acetonitrile;
Basic condition: A: 0.05% NH3.H2O in H2O; B: acetonitrile.
Mass spectra (MS): generally only ions which indicate the parent mass are reported, and unless otherwise stated the mass ion quoted is the positive mass ion (M+H)+.
NMR Spectra were obtained using Bruker Avance 400 MHz.
All reactions involving air-sensitive reagents were performed under an argon atmosphere. Reagents were used as received from commercial suppliers without further purification unless otherwise noted.
The titled compound was synthesized according to the following scheme:
Methylamine solution (355 g, 2.87 mol, 25% in ethanol) was added dropwise to 2,4-difluoronitrobenzene (147 g, 0.92 mol) at 0° C. over 15 min. After completion of the addition, the reaction mixture was stirred at 0° C. for 2 h. The solution was diluted with ethanol (500 mL) and poured into 2 L of ice-water. The resulting precipitates were collected by filtration and dried in vacuo to give 5-fluoro-N-methyl-2-nitro-aniline (143 g, 63% yield) as a yellow solid.
To the suspension of sodium hydride (141 g, 3.5 mol, 60% dispersion in mineral oil) in dry tetrahydrofuran (2 L) was added 5-fluoro-N-methyl-2-nitro-aniline (60 g, 0.35 mol, compound A1.2) portion wise at 0° C. The solution was stirred at 0° C. for 1 h. Then the solution of di-tert-butyl dicarbonate (115 g, 0.53 mol) in tetrahydrofuran (0.5 L) was added dropwise and the reaction continued for another 15 h at 15° C. The reaction mixture was poured into 1.6 L of ice-water and extracted with EtOAc (1.6 L) two times. Combined organics was dried over anhy. Na2SO4, filtered, and concentrated in vacuo. The crude product was purified by flash chromatography (silica gel, 1-5% ethyl acetate in petroleum ether) to give tert-butyl N-(5-fluoro-2-nitro-phenyl)-N-methyl-carbamate (70 g, 73% yield) as a yellow solid. MS (ESI): 293.0 ([M+Na]+), 171.0 ([M-C4H8—CO2+H]+).
To the solution of tert-butyl N-(5-fluoro-2-nitro-phenyl)-N-methyl-carbamate (70 g, 259 mmol, compound A1.3) in MeOH (1 L) was added palladium on carbon (5 g, 10 wt. % loading). The reaction mixture was stirred at 16° C. for 18 h under H2 atmosphere (50 psi). The remaining palladium catalyst was removed by filtration. The filtrate was concentrated in vacuo to give tert-butyl N-(2-amino-5-fluoro-phenyl)-N-methyl-carbamate (60 g, 96% yield) as a white solid. MS (ESI): 263.1 ([M+Na]+), 185.0 ([M-C4H8+H]+), 141.0 ([M-C4H8—CO2+H]+).
To the solution of tert-butyl N-(2-amino-5-fluoro-phenyl)-N-methyl-carbamate (80 g, 333 mmol, compound A1.4) and 2,3,5-trichloropyridine (66.8 g, 366 mmol, CAS: 16063-70-0) in dioxane (2 L) were added cesium carbonate (217 g, 666 mmol), palladium(II) acetate (3.74 g, 16.7 mmol), and BINAP (20.7 g, 33.3 mmol, CAS: 98327-87-8). Reaction continued at 120° C. for 16 h under nitrogen atmosphere. Then the reaction mixture was cooled to room temperature and diluted with EtOAc (800 mL). The precipitate was removed by filtration. The filtrate was concentrated in vacuo and the residue was purified by flash chromatography (silica gel, 0.2% to 5% EtOAc in petroleum ether) to give tert-butyl N-[2-[(3,5-dichloro-2-pyridyl)amino]-5-fluoro-phenyl]-N-methyl-carbamate (75 g, 58% yield). MS (ESI): 390.1 ([{37Cl}M+H]+), 388.1 ([{37Cl+35Cl}M+H]+), 386.1 ([{35Cl}M+H]+).
To the solution of tert-butyl N-[2-[(3,5-dichloro-2-pyridyl)amino]-5-fluoro-phenyl]-N-methyl-carbamate (5.0 g, 12.95 mmol) and DBU (3.94 g, 25.9 mmol, CAS: 6674-22-2) in the mixture of o-xylene (7.5 mL) and N,N-Dimethylacetamide (7.5 mL) were added palladium(II) acetate (727 mg, 3.24 mmol) and tricyclohexylphosphine tetrafluoroborate (2.38 g, 6.48 mmol) under nitrogen atmosphere. The reaction mixture was stirred at 160° C. for 6 h, then cooled to room temperature and poured into water (100 mL). Then the mixture was extracted with EtOAc (200 mL) and the organic layer was collected and washed with water (50 mL) two times and brine (30 mL) two times, dried over anhy. Na2SO4 and filtered. The separated organic layer was concentrated in vacuo and the residue was purified by flash chromatography (silica gel, 0.5% to 20% EtOAc in dichloromethane) to give tert-butyl N-(3-chloro-6-fluoro-9H-pyrido[2,3-b]indol-8-yl)-N-methyl-carbamate (873 mg, 19.3% yield) as a yellow solid. MS (ESI): 352.1 ([{37Cl}M+H]+), 350.1 ([{35Cl}M+H]+).
To the solution of tert-butyl N-(3-chloro-6-fluoro-9H-pyrido[2,3-b]indol-8-yl)-N-methyl-carbamate (4.8 g, 13.7 mmol, compound A1.5) in dichloromethane (200 mL) was added 3-chloroperbenzoic acid (9.47 g, 54.9 mmol) at 0° C. under nitrogen atmosphere. Reaction continued at 30° C. for 12 h. The reaction mixture was poured into sodium sulfite aqueous solution (10%, 150 mL) and stirred for 1 h followed by extraction with EtOAc (750 mL) three times. Combined organics was washed by sodium bicarbonate aqueous solution (5N, 200 mL) and brine (250 mL), then dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give tert-butyl N-(3-chloro-6-fluoro-1-oxido-9H-pyrido[2,3-b]indol-1-ium-8-yl)-N-methyl-carbamate (4.8 g, yield: 96% yield) as a brown solid. MS (ESI): 368.1 ([{37Cl}M+H]+), 366.1 ([{35Cl}M+H]+).
To the solution of tert-butyl N-(3-chloro-6-fluoro-1-oxido-9H-pyrido[2,3-b]indol-1-ium-8-yl)-N-methyl-carbamate (4.8 g, 13.1 mmol) in dimethylformamide (100 mL) was added phosphorus(V) oxychloride (22.1 g, 144 mmol) dropwise at −5° C. The mixture was stirred at −5° C. to 0° C. for 1 h then poured into sodium bicarbonate aqueous solution (saturated, 350 mL) at 0° C. The mixture was extracted by EtOAc (500 mL) three times and the combined organics were washed with water (200 mL) three times and brine (150 mL) two times, dried over anhy. Na2SO4, filtered, and concentrated in vacuo. The crude product was washed with MeOH (120 mL) to give tert-butyl N-(3,4-dichloro-6-fluoro-9H-pyrido[2,3-b]indol-8-yl)-N-methyl-carbamate (2.16 g, 42.9% yield) as a pale yellow solid. MS (ESI): 388.1 ([{37Cl+37Cl}M+H]+), 386.1 ([{37Cl+35Cl}M+H]+), 384.1 ([{35Cl+35Cl}M+H]+). 1H NMR (400 MHz, DMSO-d6) δ ppm: 12.55 (s, 1H), 8.65 (s, 1H), 8.05 (d, J=7.0 Hz, 1H) 7.49 (dd, J=10.3, 2.5 Hz, 1H) 3.26 (s, 3H) 1.19-1.62 (m, 9H).
tert-Butyl N-(3,4-dichloro-6-fluoro-9H-pyrido[2,3-b]indol-8-yl)-N-ethyl-carbamate (Intermediate A2) was prepared in analogy to Intermediate A1, by replacing methylamine solution with ethylamine solution (30% in ethanol) in step (a). MS (ESI): 402.0 ([{37Cl+37Cl}M+H]+), 400.0 ([{37Cl+35Cl}M+H]+), 398.0 ([{35Cl+35Cl}M+H]+). 1H NMR (400 MHz, DMSO-d6) δ ppm: 12.57 (s, 1H), 8.65 (s, 1H), 8.08 (d, 1H) 7.42 (m, 1H) 3.67 (br, 2H) 1.06-1.51 (m, 12H).
tert-Butyl N-(3,4-dichloro-5,6-difluoro-9H-pyrido[2,3-b]indol-8-yl)-N-methyl-carbamate (Intermediate A3) was prepared in analogy to Intermediate A1, by replacing 2,4-difluoronitrobenzene with 2,4,5-trifluoronitrobenzene in step (a). MS (ESI): 406.1 ([{37Cl+37Cl}M+H]+), 404.1 ([{37Cl+35Cl}M+H]+), 402.1 ([{35Cl+35Cl}M+H]+). 1H NMR (400 MHz, DMSO-d6) δ ppm: 12.84 (br. s, 1H), 8.64 (s, 1H), 7.70 (m, 1H), 3.22 (s, 3H), 1.22-1.50 (m, 9H).
tert-Butyl N-(3,4-dichloro-6,7-difluoro-9H-pyrido[2,3-b]indol-8-yl)-N-methyl-carbamate (Intermediate A4) was prepared in analogy to Intermediate A1, by replacing 2,4-difluoronitrobenzene with 2,3,4-trifluoronitrobenzene in step (a). MS (ESI): 406.2 ([{37Cl+37Cl}M+H]+), 404.2 ([{37Cl+35Cl}M+H]+), 402.1 ([{35Cl+35Cl}M+H]+). 1H NMR (400 MHz, DMSO-d6) δ ppm: 12.61 (br. s, 1H), 8.59 (s, 1H), 8.26 (m, 1H), 3.04 (s, 3H), 1.22-1.50 (m, 9H).
tert-Butyl N-(3,4-dichloro-5,6-difluoro-9H-pyrido[2,3-b]indol-8-yl)-N-ethyl-carbamate (Intermediate A5) was prepared in analogy to Intermediate A1, by replacing 2,4-difluoronitrobenzene with 2,4,5-trifluoronitrobenzene, and methylamine solution with ethylamine solution (30% in EtOH) in step (a). 1H NMR (400 MHz, DMSO-d6) δ ppm: 12.86 (s, 1H), 8.67 (s, 1H), 7.65 (m, 1H) 3.62 (m, 2H) 1.05-1.49 (m, 12H). MS (ESI): 420.1 ([{37Cl+37Cl}M+H]+), 418.1 ([{37Cl+35Cl}M+H]+), 416.2 ([{35Cl+35Cl}M+H]+).
The title compound was prepared in analogy to Intermediate A1, by replacing 2,4-difluoronitrobenzene with 4-chloro-2-fluoro-1-nitrobenzene in step (a), replacing methylamine solution with ethylamine solution (30% in EtOH) in step (a), and replacing 2,3,5-trichloropyridine with 2,3,5-tribromopyridine in step (d). MS (ESI): 463.9 ([{37Cl+37Cl+81Br}M+H]+), 461.9 ([{37Cl+37Cl+79Br}/{35Cl+37Cl+81Br}M+H]+), 459.9 ([{35Cl+37Cl+79Br}/{35Cl+35Cl+81Br}M+H]+), 457.9 ([{35Cl+35Cl+79Br}M+H]+).
The titled compound was synthesized according to the following scheme:
To the solution of 5-bromo-2-chloropyrimidine (3.0 g, 15.6 mmol, compound B1.1) and 4-amino-1-Boc-piperidine (4.7 g, 23.4 mmol, compound B1.2) in THF (5.0 mL) was added DIPEA (6.0 g, 48.9 mol). The reaction continued at 60° C. for 12 h. The mixture was cooled to room temperature and concentrated in vacuo, the residue was purified by column chromatography on silica gel (10% EtOAc in petroleum ether) to give tert-butyl 4-[(5-bromopyrimidin-2-yl)amino]piperidine-1-carboxylate (2.2 g, 39% yield) as a yellow oil. MS (ESI): 359.1 ([{81Br}M+H]+), 357.1 ([{79Br}M+H]+).
To the solution of tert-butyl 4-[(5-bromopyrimidin-2-yl)amino]piperidine-1-carboxylate (2.0 g, 5.6 mmol, compound B1.4), bis(pinacolato)diboron (2.8 g, 11.2 mmol) in dioxane (8.0 mL) were added potassium acetate (1.45 g, 16.8 mmol), Pd2dba3 (514 mg, 0.56 mmol), and Xphos (267 mg, 0.56 mmol). The reaction continued under nitrogen atmosphere at 100° C. for 12 h. Then the mixture was cooled to room temperature, poured into water (100 mL), and extracted with EtOAc (100 mL) for three times. The organic layer was washed with water (50 mL), dried over Na2SO4, and concentrated in vacuo to obtain crude [2-[(1-tert-butoxycarbonyl-4-piperidyl)amino]pyrimidin-5-yl]boronic acid (1.2 g, 62% yield) as a white solid. MS (ESI): 323.2 ([M+H]+).
The titled compound was synthesized according to the following scheme:
To the solution of 5-bromo-2-chloropyrimidine (1.9 g, 10 mmol, compound B2.1) and methyl 2,2-dimethyl-3-hydroxypropionate (2.6 g, 20 mmol, compound B2.2) in NMP (10 mL) was added Cs2CO3 (4.2 g, 20 mmol). The reaction continued at 120° C. for 2 h in the microwave. The mixture was cooled to room temperature and poured into water (100 mL). The mixture was extracted by EtOAc (100 mL) for three times. The organic layer was dried over anhy. Na2SO4, filtered, and concentrated in vacuo, the residue was purified by column chromatography on silica gel (10% to 25% ethyl acetate in petroleum ether) to give methyl 3-(5-bromopyrimidin-2-yl)oxy-2,2-dimethyl-propanoate (0.88 g, 29% yield) as white solid. MS (ESI): 291.1 ([{81Br}M+H]+), 289.1 ([{79Br}M+H]+).
To the solution of methyl 3-(5-bromopyrimidin-2-yl)oxy-2,2-dimethyl-propanoate (0.40 g, 1.4 mmol, compound B2.3), bis(pinacolato)diboron (0.42 g, 1.7 mmol) in dioxane (2 mL) were added potassium acetate (0.41 g, 4.2 mmol) and Pd(dppf)Cl2 (51 mg, 0.07 mmol). The reaction continued under nitrogen atmosphere at 140° C. for 2 h in the microwave. Then the mixture was cooled to room temperature and concentrated in vacuo, the residue was purified by flash chromatography on silica gel (10% to 25% ethyl acetate in petroleum ether) to give methyl 2,2-dimethyl-3-[5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyrimidin-2-yl]oxy-propanoate (195 mg, 42% yield) as a white solid. MS (ESI): 337.3 ([M+H]+).
The title compound was prepared in analogy to Intermediate B2, by replacing methyl 2,2-dimethyl-3-hydroxypropionate (compound B2.2) with methyl 1-(aminomethyl) cyclopropanecarboxylatehydrochloride in step (a). MS (ESI): 252.0 ([M+H]+).
The title compound was prepared in analogy to Intermediate B2, by replacing methyl 2,2-dimethyl-3-hydroxypropionate (compound B2.2) with methyl 3-amino-2,2-dimethyl-propanoate in step (a). MS (ESI): 254.2 ([M+H]+).
The title compound was prepared in analogy to Intermediate B2, by replacing methyl 2,2-dimethyl-3-hydroxypropionate with 1-Boc-3-hydroxypyrrolidine. MS (ESI): 310.1 ([M+H]+).
The title compound was prepared in analogy to Intermediate B2, by replacing methyl 2,2-dimethyl-3-hydroxypropionate with 1-Boc-3-hydroxyazetidine, Cs2CO3 with NaH, NMP with DMF, and the reaction was conducted for 3 h at room temperature instead of 120° C. for 2 h in step (a). MS (ESI): 296.1 ([M+H]+).
The title compound was prepared in analogy to Intermediate B2, by replacing methyl 2,2-dimethyl-3-hydroxypropionate with 1-Boc-3-aminopyrrolidine, Cs2CO3 with DIPEA, NMP with THF, and the reaction was conducted at 65° C. for 12 h instead of 120° C. for 2 h in step (a). MS (ESI): 309.2 ([M+H]+).
The title compound was prepared in analogy to Intermediate B2, by replacing methyl 2,2-dimethyl-3-hydroxypropionate with 3-aminotetrahydrofuran, Cs2CO3 with DIPEA, NMP with THF, and the reaction was conducted at 65° C. for 12 h instead of 120° C. for 2 h in step (a). MS (ESI): 210.2 ([M+H]+).
The title compound was prepared in analogy to Intermediate B2, by replacing methyl 2,2-dimethyl-3-hydroxypropionate with N-Boc-2-amino-2-methyl-1-propanol in step (a). MS (ESI): 312.2 ([M+H]+).
The titled compound was prepared in analogy to Intermediate B4, by replacing ethyl 1-(5-bromopyrimidin-2-yl)cyclopropanecarboxylate (compound B4.1) with N-Boc-5-bromo-2-pyrimidinemethanamine (Bepharm, catalog number: B220284). MS (ESI): 254.1 ([M+H]+).
The titled compound was synthesized according to the following scheme:
The mixture of DIPEA (1 mL), (5-bromopyrimidin-2-yl)methanol (compound B11.1, 125 mg, 661 μmol) and bromo(methoxy)methane (413 mg, 3.31 mmol) in 10 mL DCM was stirred at r.t. overnight. The reaction mixture was diluted with EtOAc (50 mL) and washed with water (50 mL). The organic layer was dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give crude 5-bromo-2-(methoxymethoxymethyl)pyrimidine (160 mg, 99% yield) as light yellow oil.
The titled compound was prepared in analogy to Intermediate B4, by replacing ethyl 1-(5-bromopyrimidin-2-yl)cyclopropanecarboxylate (compound B4.1) with 5-bromo-2-(methoxymethoxymethyl)pyrimidine (crude, prepared from step (a)). MS (ESI): 199.1 ([M+H]+).
The titled compound was synthesized according to the following scheme:
To a solution of ethyl 3-(trifluoromethyl)-1H-pyrazole-4-carboxylate (4 g, 19.2 mmol, compound C1.1) in DMF (50 mL) was added NaH (1.5 g, 38.5 mmol) at 0° C. The mixture was stirred for 1 h before a solution of PMBCl (3.6 g, 23.1 mmol) was added. After the reaction was continued at room temperature for 3 h, it was poured into water (100 mL), and extracted with EtOAc (200 mL) twice. The combined organics were dried over anhy. Na2SO4, filtered, and concentrated in vacuo, and the residue was purified by flash chromatography on silica gel (petroleum ether:EtOAc=20:1˜5:1) to give ethyl 1-[(4-methoxyphenyl)methyl]-3-(trifluoromethyl)pyrazole-4-carboxylate (3.8 g, 60% yield) as a yellow solid. MS (ESI): 329.2 ([M+H]+).
The solution of ethyl 1-[(4-methoxyphenyl)methyl]-3-(trifluoromethyl)pyrazole-4-carboxylate (3.8 g, 11.3 mmol) and NaOH (9 g, 225 mmol) in EtOH (30 mL) and water (15 mL) was stirred at 60° C. for 2 h. After the solution was cooled back to room temperature, aq. HCl solution (28% in water) was added to adjust pH to about 3. The mixture was then concentrated in vacuo and the residue was re-dissolved in EtOAc (100 mL), and washed with water (100 mL). The organic layer was dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give 1-[(4-methoxyphenyl)methyl]-3-(trifluoromethyl)pyrazole-4-carboxylic acid (3.3 g, 97% yield) as a yellow solid. MS (ESI): 301.2 ([M+H]+).
The solution of 1-[(4-methoxyphenyl)methyl]-3-(trifluoromethyl)pyrazole-4-carboxylic acid (3.2 g, 10.7 mmol), NH4Cl (2.85 g, 53.3 mmol), HOBt (1.73 g, 12.8 mmol), PyBOP (6.66 g, 12.8 mmol), and DIPEA (8.25 g, 64.0 mmol) in DMF (30.0 mL) was stirred at 30° C. under N2 atmosphere for 1 h. Then the mixture was poured into water (100 mL) and extracted with EtOAc (100 mL) three times. The combined organics was dried over anhy. Na2SO4, filtered, and concentrated in vacuo, and the residue was purified by prep-HPLC to give 1-[(4-methoxyphenyl)methyl]-3-(trifluoromethyl)pyrazole-4-carboxamide (3.0 g, 94% yield) as yellow oil. MS (ESI): 300.2 ([M+H]+).
To a solution of 1-[(4-methoxyphenyl)methyl]-3-(trifluoromethyl)pyrazole-4-carboxamide (2.9 g, 9.7 mmol) in THF (25 mL) was added LAH (1.84 g, 48.5 mmol) at 0° C. The reaction was stirred at 80° C. for 2 h under N2 atmosphere. After the mixture was cooled to 0° C., water (1.8 mL), 15% aqueous NaOH (3.6 mL), and then water (1.8 mL) were added sequentially. Then the mixture was diluted with EtOAc (200 mL) and filtered. The filtrate was dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give [1-[(4-methoxyphenyl)methyl]-3-(trifluoromethyl)pyrazol-4-yl]methanamine (2.6 g, 94% yield) as clear oil. MS (ESI): 286.2 ([M+H]+).
To a solution of [1-[(4-methoxyphenyl)methyl]-3-(trifluoromethyl)pyrazol-4-yl]methanamine (2.5 g, 8.8 mmol) in MeCN (40 mL) and water (10 mL) was added ceric ammonium nitrate (19.2 g, 35.0 mmol). The reaction was stirred at room temperature for 2 h. The mixture was basified to pH ˜9 with NaOH solid and then diluted with MeOH (400 mL). The mixture was filtered and the filtrate was concentrated in vacuo to give crude [3-(trifluoromethyl)-1H-pyrazol-4-yl]methanamine (1.5 g) as a yellow solid.
The mixture of crude [3-(trifluoromethyl)-1H-pyrazol-4-yl]methanamine (1.5 g, 8.5 mmol), Boc2O (2.78 g, 12.7 mmol) and DIPEA (3.28 g, 25.4 mmol) in MeOH (50 mL) was stirred at room temperature for 1 h. After the mixture was concentrated in vacuo, the residue was re-suspended in water (100 mL), and extracted with EtOAc (100 mL) three times. The combined organics was washed with brine (50 mL) twice, dried over anhy. Na2SO4, filtered, and concentrated in vacuo, and the residue was purified by prep-HPLC to give tert-butyl N-[[3-(trifluoromethyl)-1H-pyrazol-4-yl]methyl]carbamate (1.05 g, 47% yield) as a white solid. MS (ESI): 266.3 ([M+H]+).
The titled compound was synthesized according to the following scheme:
To the solution of N-Boc-3-pyrrolidinone (1.0 g, 5.4 mmol) in THF (20 mL) was added LDA (6.5 mL, 1 M in THF/hexanes, 6.5 mmol) and ethyl trifluoroacetate (1.0 g, 7.0 mmol) at −78° C. Reaction was allowed to warm up to room temperature slowly and stirred at room temperature for 12 h. Then the mixture was poured into water (100 mL) and extracted with EtOAc (100 mL) three times. The combined organics was washed with brine (100 mL), dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give crude tert-butyl 3-oxo-4-(2,2,2-trifluoroacetyl)pyrrolidine-1-carboxylate (1.7 g, quantitive yield) as yellow oil, which was used in the next step without purification.
The solution of tert-butyl 3-oxo-4-(2,2,2-trifluoroacetyl)pyrrolidine-1-carboxylate (1.7 g, 5.4 mmol, prepared from step (a)) and hydrazine monohydrate (0.35 g, 7.0 mmol) in EtOH (20 mL) was stirred at 60° C. for 12 h. Then the mixture was cooled to room temperature, concentrated in vacuo, and then dissolved in EtOAc (100 mL). The solution was washed with brine (100 mL), dried over anhy. Na2SO4, filtered, and concentrated in vacuo, the residue was purified by prep-HPLC to give tert-butyl 3-(trifluoromethyl)-4,6-dihydro-1H-pyrrolo[3,4-c]pyrazole-5-carboxylate (160 mg, 11% yield).
1H NMR (400 MHz, DMSO-d6) δ ppm: 13.65 (s, 1H), 4.42-4.53 (m, 4H), 1.50 (s, 9H). MS (ESI): 278.1 ([M+H]+).
The titled compound was synthesized according to the following scheme:
To a solution of tert-butyl 4,6-dihydropyrrolo[3,4-c]pyrazole-5(1H)-carboxylate (1 g, 4.8 mmol), K2CO3 (1.4 g, 10.6 mmol) in DMF (20 mL) was added I2 (2.4 g, 9.6 mmol) in DMF (10 mL) at room temperature. Reaction continued for 4 h, then another portion of K2CO3 (1.4 g, 10.6 mmol) and I2 (2.4 g, 9.6 mmol) was added. Reaction continued for another 14 h, and was quenched with saturated Na2S2O3 solution (20 mL). The mixture was diluted with water (100 mL), extracted with EtOAc (80 mL) three times. The combined organics was washed with brine (50 mL) twice, dried over anhy. Na2SO4, filtered, and concentrated in vacuo, the residue was purified by flash chromatography to give tert-butyl 3-iodo-4,6-dihydro-1H-pyrrolo[3,4-c]pyrazole-5-carboxylate (800 mg, 50% yield) as yellow solid. MS (ESI): 336.0 ([M+H]+).
To a solution of tert-butyl 3-iodo-4,6-dihydro-1H-pyrrolo[3,4-c]pyrazole-5-carboxylate (700 mg, 2.1 mmol) in DMF (20 mL) were added NaH (125 mg, 3.1 mmol) and PMBCl (391 mg, 2.5 mmol) at 0° C. Reaction continued at room temperature for 18 h. The solution was quenched with saturated NH4Cl (20 mL), diluted with water (100 mL), and extracted with EtOAc (50 mL) three times. The combined organics was washed with brine (30 mL), dried over anhy. Na2SO4, filtered, and concentrated in vacuo. The crude product was purified by silica gel chromatography to give tert-butyl 3-iodo-1-[(4-methoxyphenyl)methyl]-4,6-dihydropyrrolo[3,4-c]pyrazole-5-carboxylate (900 mg, 95% yield) as yellow oil. MS (ESI): 456.1 ([M+H]+).
To the solution of tert-butyl 3-iodo-1-[(4-methoxyphenyl)methyl]-4,6-dihydropyrrolo[3,4-c]pyrazole-5-carboxylate (900 mg, 1.98 mmol), Zn(CN)2 (459 mg, 3.96 mmol), Zn (32 mg, 0.5 mmol), CuI (564 mg, 2.97 mmol) in DMA (20 mL) was added Pd(dppf)2Cl2 (163 mg, 0.20 mmol) at room temperature. Reaction continued at 120° C. for 18 h under N2 atmosphere. The mixture was cooled to room temperature and poured into saturated NH4Cl (50 mL), diluted with water (100 mL), and extracted with EtOAc (50 mL) three times. The combined organics was washed with brine (50 mL), dried over anhy. Na2SO4, and concentrated in vacuo. The crude product was purified by silica gel chromatography to give tert-butyl 3-cyano-1-[(4-methoxyphenyl)methyl]-4,6-dihydropyrrolo[3,4-c]pyrazole-5-carboxylate (500 mg, 71% yield) as colorless oil. MS (ESI): 355.2 ([M+H]+).
To the solution of tert-butyl 3-cyano-1-[(4-methoxyphenyl)methyl]-4,6-dihydropyrrolo[3,4-c]pyrazole-5-carboxylate (500 mg, 1.4 mmol) in the mixture of MeCN (20 mL) and water (4 mL) was added CAN (3.8 g, 7.0 mmol) at room temperature. The solution was stirred for 4 h before diluted with water (50 mL) and extracted with EtOAc (50 mL) three times. The combined organics was washed with brine (50 mL), dried over anhy. Na2SO4, filtered, and concentrated in vacuo. The crude product was purified by silica gel chromatography to give tert-butyl 3-cyano-4,6-dihydro-1H-pyrrolo[3,4-c]pyrazole-5-carboxylate (150 mg, 46% yield) as a white solid. MS (ESI): 235.1 ([M+H]+).
The titled compound was synthesized in analogy to Intermediate C3, by replacing tert-butyl 4,6-dihydropyrrolo[3,4-c]pyrazole-5(1H)-carboxylate with tert-Butyl 4,5-dihydro-1H-pyrazolo[3,4-c]pyridine-6(7H)-carboxylate-6(7H)-carboxylate in step (a). MS (ESI): 249.1 ([M+H]+).
To the solution of tert-butyl N-(3,4-dichloro-6-fluoro-9H-pyrido[2,3-b]indol-8-yl)-N-ethyl-carbamate (Intermediate A2, 80 mg, 0.2 mmol, as the “CORE” in table 1) in DMSO (1 mL) were added 3-(trifluoromethyl)pyrazole (54.4 mg, 0.40 mmol, as the “NUCLEOPHILE” in table 1) and K2CO3 (82.9 mg, 0.60 mmol). The mixture was stirred at 120° C. for 12 h. The reaction mixture was cooled to room temperature, filtered through thin celite pad, and concentrated in vacuo. The residue was purified by prep-HPLC to give tert-butyl N-[3-chloro-6-fluoro-4-[3-(trifluoromethyl)pyrazol-1-yl]-9H-pyrido[2,3-b]indol-8-yl]-N-ethyl-carbamate (85 mg, 86% yield) as a white solid. MS (ESI): 500.2 ([{37Cl}M+H]+), 498.2 ([{35Cl}M+H]+).
A solution of tert-butyl N-[3-chloro-6-fluoro-4-[3-(trifluoromethyl)pyrazol-1-yl]-9H-pyrido[2,3-b]indol-8-yl]-N-ethyl-carbamate (60 mg, 0.12 mmol), 3-cyanopyridine-5-boronic acid pinacol ester (55.7 mg, 0.24 mmol, as the “BORONIC REAGENT” in table 1), Pd2dba3 (21.9 mg, 0.024 mmol) and XPhos (11.4 mg, 0.024 mmol), as the “CATALYST” in table 1, and potassium phosphate tribasic (144.7 mg, 0.69 mmol) in dioxane (4 mL) and water (0.4 mL) was stirred at 100° C. for 12 h under N2 atmosphere. The mixture was cooled to room temperature and poured into water (50 mL). Then the mixture was extracted with EtOAc (50 mL) three times. The combined organics was dried over anhy. Na2SO4, filtered, and concentrated in vacuo. The residue was purified by prep-HPLC to give tert-butyl N-[3-(5-cyano-3-pyridyl)-6-fluoro-4-[3-(trifluoromethyl)pyrazol-1-yl]-9H-pyrido[2,3-b]indol-8-yl]-N-ethyl-carbamate (45.3 mg, 66% yield) as a yellow oil. MS (ESI): 566.3 ([M+H]+).
To the solution of tert-butyl N-[3-(5-cyano-3-pyridyl)-6-fluoro-4-[3-(trifluoromethyl)pyrazol-1-yl]-9H-pyrido[2,3-b]indol-8-yl]-N-ethyl-carbamate (45 mg, 0.08 mmol) in DCM (3 mL) was added TFA (2 mL). The solution was stirred at room temperature for 2 h before it was concentrated in vacuo. The residue was purified by prep-HPLC to give 5-[4-[(3R)-3-aminopyrrolidin-1-yl]-6-fluoro-8-(methylamino)-9H-pyrido[2,3-b]indol-3-yl]pyridine-3-carbonitrile (30 mg, 81% yield) as a yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 12.19 (s, 1H), 8.98 (d, J=2.0 Hz, 1H), 8.76 (s, 1H), 8.57 (d, J=2.0 Hz, 1H), 8.33 (s, 1H), 8.12 (s, 1H), 7.13 (d, J=2.4 Hz, 1H), 6.48 (m, 1H), 5.92 (m, 1H), 5.81 (m, 1H), 3.25 (m, 2H), 1.32 (t, J=6.8 Hz, 3H). MS (ESI): 466.1 ([M+H]+).
The following examples were prepared in analogy to Example 1.01, replacing tert-butyl N-(3,4-dichloro-6-fluoro-9H-pyrido[2,3-b]indol-8-yl)-N-ethyl-carbamate (Intermediate A2) as the “CORE” in step (a), 3-(trifluoromethyl)pyrazole as the “NUCLEOPHILE” in step (a), and 3-cyanopyridine-5-boronic acid pinacol ester as the “BORONIC REAGENT” in step (b), Pd2dba3 and XPhos as the “CATALYST” in step (b) with the reagents indicated in Table 1 respectively.
1H NMR and MS (ESI)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.63 (s, 1H), 8.40 (s, 2H), 8.06 (s, 1H), 6.97 (s, 1H), 6.5 (m, 1H), 5.95 (m, 1H), 4.04 (s, 3H), 2.99 (s, 3H). MS (ESI): 458.1 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.19 (s, 1H), 9.00 (d, J = 2.0 Hz, 1H), 8.82 (s, 1H), 8.77 (s, 1H), 8.58 (s, 1H), 8.44 (s, 1H), 8.12 (d, J = 2.0 Hz, 1H), 6.51 (m, 1H), 5.84 (m, 1H), 3.26 (q, J = 7.2 Hz, 2H), 1.32 (t, J = 7.2 Hz, 3H). MS (ESI): 466.1 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 8.70 (s, 1H), 8.38 (s, 2H), 8.29 (s, 1H), 7.10 (s, 1H), 6.46 (m, 1H), 5.72 (m, 1H), 3.92 (s, 3H), 3.24 (m, 2H), 1.32 (m, 3H). MS (ESI): 472.2 ([M + H]+)
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.22 (s, 1H), 9.01 (d, J = 1.2 Hz, 1H), 8.77 (s, 1H), 8.56 (d, J = 2.4 Hz, 1H), 8.43 (d, J = 2.4 Hz, 1H), 8.21 (s, 1H), 7.37 (d, J = 2.4 Hz, 1H), 6.52 (m, 1H), 5.76 (m, 1H), 3.27 (q, J = 7.2 Hz, 2H), 1.33 (t, J = 7.2 Hz, 3H). MS (ESI): 423.2 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.01 (s, 1H), 8.66 (s, 1H), 8.30 (d, 1H), 8.01 (s, 2H), 7.13 (d, 1H), 6.80 (s, 2H), 6.47 (m, 1H), 5.86 (m, 1H), 5.67 (m, 1H), 3.23-3.28 (m, 2H), 1.32 (m, 3H). MS (ESI): 457.2 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.08 (s, 1H), 8.67 (s, 1H), 8.19-8.30 (m, 2H), 7.46 (m, 1H), 7.24 (d, 1H), 7.08 (d, 1H), 6.48 (m, 1H), 5.85-5.91 (m, 1H), 5.69 (m, 1H), 3.25-3.28 (q, 2H), 2.47 (s, 3H), 1.32 (t, 3H). MS (ESI): 455.2 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.10 (s, 1H), 8.99 (s, 1H), 8.81 (s, 1H), 8.77 (s, 1H), 8.58 (d, J = 2.0 Hz, 1H), 8.43 (s, 1H), 8.12 (d, J = 2.0 Hz, 1H), 6.49 (m, 1H), 6.03 (d, J = 4.4 Hz, 1H), 5.83 (m, 1H), 2.92 (d, J = 4.0 Hz, 3H). MS (ESI): 452.2 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.18 (s, 1H), 9.01 (s, 1H), 8.77 (s, 1H), 8.56 (s, 1H), 8.43 (d, J = 2.0 Hz, 1H), 8.21 (s, 1H), 7.37 (d, J = 2.4 Hz, 1H), 6.51 (d, J = 12.0 Hz, 1H), 6.09 (br, 1H), 5.77 (d, J = 8.8 Hz, 1H), 2.93 (s, 3H). MS (ESI): 409.2 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.05 (br. s., 1H), 8.66 (s, 1H), 8.34 (d, 1H), 8.06 (br. s., 1H), 7.42 (d, 1H), 7.15 (d, 1H), 6.47 (m, 1H), 5.87 (br. s., 1H), 5.66 (m, 1H), 5.27- 5.36 (m, 1H), 3.25 (br. s., 2H), 3.10 (br. s., 1H), 2.65- 2.76 (m, 2H), 1.97-2.06 (m, 2H), 1.87 (d, 2H), 1.46 (d, 2H), 1.27-1.34 (m, 3H). MS (ESI): 540.3 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.17 (s, 1H), 9.04 (m, 1H), 8.91 (s, 1H), 8.70 (d, 1H), 8.44 (d, 1H), 8.29 (m, 2H), 7.82 (m, 1H), 7.07 (d, 1H), 6.51 (m, 1H), 5.90 (br. s., 1H), 5.80 (m, 1H), 3.28 (d, 2H), 1.34 (t, 3H). MS (ESI): 492.2 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.18 (br. s., 1H), 9.14 (s, 1H), 8.78 (s, 1H), 8.60 (s, 2H), 8.37 (d, 1H), 7.14 (d, 1H), 6.51 (m, 1H), 5.90 (br. s., 1H), 5.78 (m, 1H), 3.22- 3.29 (m, 2H), 1.33 (m, 3H). MS (ESI): 442.2 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.01 (s, 1H), 8.66 (s, 1H), 8.31 (d, 1H), 8.06 (br. s., 1H), 7.33 (br. s., 1H), 7.14 (d, 1H), 6.47 (m, 1H), 5.85 (br. s., 1H), 5.67 (m, 1H), 5.33 (m, 1H), 3.44 (s, 3H), 3.28 (br. s., 2H), 1.32 (m, 3H). MS (ESI): 515.3 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.19 (br. s., 1 H), 9.01 (d, J = 1.6 Hz, 1H), 8.75 (s, 1 H), 8.56 (d, J = 2.0 Hz, 1H), 8.30 (m, 3H), 8.20 (s, 1H), 6.50 (m, 1H), 6.04 (m, 1H), 4.10 (m, 2H), 2.93 (s, 3H). MS (ESI): 481.2 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.13 (s, 1H), 8.95 (d, 1H), 8.70-8.79 (m, 1H), 8.42 (d, 1H), 8.27 (d, 1H), 8.15 (s, 1H), 8.08 (m, 1H), 7.63 (br. s., 1H), 7.08 (d, 1H), 6.50 (m, 1H), 5.89 (br. s., 1H), 5.76 (m, 1H), 3.27 (m, 2H), 1.31- 1.36 (m, 3H). MS (ESI): 484.2 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.06 (br. s., 1H), 8.67 (s, 1H), 8.33 (d, 1H), 8.18 (s, 2H), 7.17 (d, 1H), 6.47 (m, 1H), 5.86 (br. s., 1H), 5.65 (m, 1H), 3.53-3.74 (m, 6H), 3.14-3.30 (m, 2H), 1.96- 2.05 (m, 2H), 1.32 (m, 3H). MS (ESI): 527.4 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.03 (s, 1H), 8.66 (s, 1H), 8.31 (s, 1H), 7.22 (br. s., 1H), 7.14 (d, 1H), 6.68 (br. s., 1H), 6.47 (m, 1H), 5.86 (br. s., 1H), 5.66 (m, 1H), 5.33 (m, 1H), 3.19-3.29 (m, 2H), 2.74 (d, 3H), 1.29-1.35 (t, 3H). MS (ESI): 471.3 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.20 (br. s., 1H), 8.76 (s, 1H), 8.56 (d, 1H), 8.33 (s, 1H), 8.22 (s, 1H), 7.57 (d, 1H), 7.22 (br. s., 1H), 6.66-6.72 (m, 1H), 6.50 (d, 1H), 5.76 (m, 1H), 3.21-3.30 (m, 2H), 1.29-1.35 (m, 3H). MS (ESI): 459.2 ([M + H]+)
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.16 (s, 1H), 9.15 (d, 1H), 8.84 (s, 1H), 8.40 (d, 1H), 8.28 (d, 1H), 8.10 (d, 1H), 7.13 (d, 1H), 6.76 (d, 1H), 6.51 (m, 1H), 5.90 (br. s., 1H), 5.81 (m, 1H), 3.22-3.31 (m, 2H), 1.30-1.37 (t, 3H). MS (ESI): 481.3 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.51 (s, 1H), 8.33 (s, 2H), 7.98 (s, 1H), 6.86 (s, 1H), 6.40 (m, 1H), 5.80 (m, 1H), 5.60 (s, 1H), 3.40 (m, 2H), 3.31 (m, 2H), 3.20 (m, 2H), 2.20 (m, 3H), 1.32 (m, 3H). MS (ESI): 527.3 ([M + H]+)
1H NMR (40 0MHz, DMSO-d6) δ ppm: 12.13 (s, 1H), 10.00 (br. s., 2H), 8.70 (s, 1H), 8.38 (s, 2H), 7.84 (s, 1H), 6.51 (m, 1H), 6.02 (m, 1H), 4.35-4.42 (m, 2H), 4.10 (s, 1H), 3.95 (s, 3H) 3.80 (m, 1H), 3.26 (m, 2H), 1.32 (t, J = 7.2 Hz, 3H). MS (ESI): 445.3 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.50 (s, 1H), 8.26 (s, 2H), 7.94 (d, 1H), 6.85 (d, 1H), 6.38 (m, 1H), 5.79 (m, 1H), 4.34 (m, 2H), 3.21 (d, 2H), 1.26-1.35 (m, 6H). MS (ESI): 486.5 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.61 (s, 1H), 8.38 (s, 2H), 8.07 (s, 1H), 6.97 (s, 1H), 6.51 (m, 1H), 5.90 (m, 1H), 5.50 (m, 1H), 4.00 (m, 2H), 3.75 (m, 2H), 3.51 (m, 1H), 3.31 (m, 2H), 1.42 (m, 3H). MS (ESI): 513.2 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.54 (s, 1H), 8.17 (s, 2H), 8.02 (s, 1H), 6.96 (s, 1H), 6.47 (d, J = 11.8 Hz, 1H), 5.86 (d, J = 9.2 Hz, 1H), 4.59 (m, 1H), 3.57 (m, 2H), 3.01 (m, 1H), 2.88 (m, 1H), 2.41 (m, 1H), 2.25 (m, 1H), 1.40- 1.43 (m, 5H). MS (ESI): 526.2 ([M + H]+)
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.03 (br. s., 1H), 8.67 (s, 1H), 8.31 (s, 1H), 8.08 (br. s, 2H), 7.66 (s, 1H), 7.14 (s, 1H), 6.46 (d, J = 11.6 Hz, 1H), 5.68 (d, J = 8.8 Hz, 1H), 3.85 (m, 3H), 3.72 (m, 1H), 3.53 (m, 1H), 3.24 (m, 2H), 2.13 (m, 1H), 1.86 (m, 1H), 1.31 (t, J = 6.8 Hz, 3H). MS (ESI): 527.3 ([M + H]+)
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.28 (s, 1H), 9.00 (d, J = 1.6 Hz, 1H), 8.74 (s, 1H), 8.55 (d, J = 2.0 Hz, 1H), 8.36 (br. s, 3H), 8.30 (s, 1 H), 8.19 (s, 1H), 6.53 (m, 1H), 6.03 (m, 1H), 4.10 (s, 2H), 3.26 (m, 2H), 1.32 (t, J = 6.8 Hz, 3H). MS (ESI): 495.3 ([M + H]+)
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.23 (s, 1H), 8.71 (s, 1H), 8.31-8.40 (m, 6H), 6.48 (m, 1H), 5.94 (m, 1H), 4.12 (s, 2H), 3.94 (s, 3H), 3.25 (q, J = 7.2 Hz, 2H), 1.32 (t, J = 7.2 Hz, 3H). MS (ESI): 501.1 ([M + H]+)
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.51 (br. s, 1H), 8.98 (s, 1H) 8.79 (s, 1H), 8.60 (s, 1H), 8.31 (s, 1H), 8.03 (s, 1H), 6.96 (s, 1H), 6.67 (m, 1H), 5.69 (s, 1H), 3.23 (m, 2H), 1.31 (t, J = 7.8 Hz, 3H). MS (ESI): 484.2 ([M + H]+)
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.43 (s, 1H), 8.74 (s, 1H), 8.32-8.35 (m, 3H), 6.97 (d, J = 2.0 Hz, 1H), 6.65 (m, 1H), 5.65 (br. s, 1H), 3.91 (s, 3H), 3.23 (m, 2H), 1.31 (t, J = 7.2 Hz, 3H). MS (ESI): 490.2 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.51 (s, 1H), 8.42-8.47 (m, 1H), 8.29-8.37 (m, 2H), 7.97 (d, 1H), 6.85 (d, 1H), 6.39 (m, 1H), 5.77 (m, 1H), 4.26 (s, 2H), 1.27-1.35 (m, 9H). MS (ESI): 529.3 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.66 (s, 1H), 8.60 (s, 2H), 8.10 (d, 1H), 6.97 (d, 1H), 6.51 (m, 1H), 5.96 (m, 1H), 1.60 (s, 6H), 1.43 (t, 3H). MS (ESI): 500.3 ([M + H]+)
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.01 (s, 1H), 8.94 (d, J = 1.6 Hz, 1H), 8.71 (s, 1H), 8.56 (d, J = 2.0 Hz, 1H), 7.97 (d, J = 2.0 Hz, 1H), 7.83 (d, J = 2.4 Hz, 1H), 6.43-6.49 (m, 2H), 6.10 (m, 1H), 5.84 (br. s, 1H), 3.26 (q, J = 7.2 Hz, 2H), 2.64 (m, 2H), 1.32 (t, J = 7.2 Hz, 3H), 1.19 (t, J = 7.6 Hz, 3H). MS (ESI): 426.1 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.72 (s, 1H), 8.38 (s, 2H), 8.32 (s, 1H), 7.14 (d, J = 2.0 Hz, 1H), 6.58 (s, 1H), 6.05 (s, 1H), 5.87 (d, J = 4.4 Hz, 1H), 3.92 (s, 3H), 3.24 (m, 2H), 1.31 (t, J = 7.2 Hz, 3H). MS (ESI): 488.0 ([{35Cl}M + H]+), 490.0 ([{37Cl}M + H]+)
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.30 (s, 1H), 10.22 (br. s, 2H), 9.05 (d, J = 1.6 Hz, 1H), 8.78 (s, 1H), 8.56 (d, J = 2.0 Hz, 1H), 8.19 (s, 1H), 6.56 (m, 1H), 6.10 (m, 1H), 4.45-4.61 (m, 2H), 4.22 (d, J = 14.8 Hz, 1H), 3.84 (d, J = 14.8 Hz, 1H), 3.28 (q, J = 7.2 Hz, 2H), 1.33 (t, J = 7.2 Hz, 3H). MS (ESI): 507.1 ([M + H]+)
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.25 (s, 1H), 10.26 (br. s., 2H), 8.73 (s, 1H), 8.43 (s, 2H), 6.53 (m, 1H), 6.04 (m, 1H), 4.62 (d, J = 13.2 Hz, 1H), 4.52 (d, J = 13.2 Hz, 1H), 4.24 (d, J = 14.8 Hz, 1H), 3.94 (d, J = 14.8 Hz, 1H), 3.27 (q, J = 7.2 Hz, 2H), 1.32 (t, J = 7.2 Hz, 3H). MS (ESI): 513.1 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.64 (s, 1H), 8.55 (s, 1H), 8.31 (s, 1H), 7.98 (d, J = 1.5 Hz, 1H), 7.82 (s, 1H), 6.92 (d, J = 2.4 Hz, 1H), 6.49 (dd, J = 12.0, 2.2 Hz, 1H), 5.93 (dd, J = 9.0, 2.2 Hz, 1H), 4.68 (s, 2H), 3.34-3.45 (m, 2H), 1.42 ppm (t, J = 7.2 Hz, 3H). MS (ESI): 471.1 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.62 (s, 1H), 8.08 (d, J = 1.6 Hz, 1H), 7.95 (br s, 1H), 7.63 (s, 1H), 7.32-7.45 (m, 1H), 6.99 (d, J = 2.4 Hz, 1H), 6.50 (dd, J = 11.9, 2.2 Hz, 1H), 5.98 (dd, J = 9.1, 2.3 Hz, 1H), 3.34-3.47 (m, 2H), 1.42 ppm (t, J = 7.2 Hz, 3H). MS (ESI): 456.1 ([M + H]+)
1H NMR (40 0MHz, MeOH-d4) δ ppm: 8.67 (s, 1H), 8.49-8.52 (m, 2H), 6.52-6.59 (m, 1H), 6.02 (dd, J = 2.20, 8.60 Hz, 1H), 4.71 (d, J = 16.44 Hz, 1H), 4.56- 4.63 (m, 1H), 4.057-4.079 (m, 1H), 4.04-4.11 (m, 4H), 3.03 (s, 3H). MS (ESI): 456.2 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.68 (s, 1H), 8.49 (s, 2H), 6.55-6.51 (d, J = 14 Hz, 1H), 5.75-5.72 (d, J = 10.8 Hz, 1H), 4.04 (s, 3H), 3.97-3.89 (m, 2H), 3.47-3.46 (m, 2H), 3.31- 3.02 (m, 4H), 1.42-1.38 (m, 3H). MS (ESI): 484.2 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.53 (s, 1H), 8.24 (d, 1H), 7.89 (d, 1H), 7.74 (s, 1H), 7.31 (m, 1H), 6.91 (d, 1H), 6.58 (m, 1H), 6.46 (m, 1H), 5.86 (m, 1H), 3.30 (m, 2H), 1.40 (t, 3H). MS (ESI): 456.2 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.84 (d, 1H), 8.64 (s, 1H), 8.58 (d, 1H), 8.02 (m, 1H), 7.96 (d, 1H), 6.83 (d, 1H), 6.49 (m, 1H), 5.99 (m, 1H), 5.17 (s, 1H), 4.58 (m, 2H), 1.41 (t, 3H). MS (ESI): 448.3 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.62 (s, 1H), 8.46 (d, J = 1.8 Hz, 1H), 7.02 (d, J = 1.5 Hz, 1H), 7.77 (dd, J = 8.0, 2.3 Hz, 1H), 7.51 (d, J = 7.9 Hz, 1H), 6.92 (d, J = 2.3 Hz, 1H), 6.50 (dd, J = 12.0, 2.2 Hz, 1H), 5.92 (dd, J = 9.0, 2.2 Hz, 1H), 4.39 (s, 2H), 3.83-3.99 (m, 4H), 3.12-3.29 (m, 6H), 1.42 (t, J = 7.2 Hz, 3H). MS (ESI): 540.2 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.78 (d, 1H), 8.56 (s, 1H), 8.31 (d, 1H), 8.04 (m, 1H), 7.87 (d, 1H), 6.79 (d, 1H), 6.39 (m, 1H), 5.79-5.86 (m, 1H), 3.15-3.20 (m, 2H), 2.84 (s, 3H), 1.26-1.35 (t, 3H). MS (ESI): 498.1 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.59- 8.70 (m, 1H), 8.21-8.36 (m, 1H), 7.89-8.06 (m, 2H), 7.20-7.37 (m, 1H), 6.96 (d, J = 2.4 Hz, 1H), 6.50 (dd, J = 11.9, 2.1 Hz, 1H), 5.90 (dd, J = 9.0, 2.1 Hz, 1H), 3.75-3.87 (m, 4H), 3.03- 3.24 (m, 4H), 2.84-3.02 (m, 2H), 1.27-1.49 (m, 3H). MS (ESI): 526.3 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.57- 8.68 (m, 2H), 8.34 (d, J = 1.8 Hz, 1H), 7.93-8.09 (m, 2H), 6.95 (d, J = 2.4 Hz, 1H), 6.43-6.57 (m, 1H), 5.93 (dd, J = 9.0, 2.2 Hz, 1H), 4.23 (s, 2H), 3.21-3.24 (m, 2H), 1.33-1.50 ppm (m, 3H). MS (ESI): 470.4 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.55- 8.64 (m, 3H), 7.99 (d, J = 1.6 Hz, 1H), 6.85 (d, J = 2.4 Hz, 1H), 6.41 (dd, J = 11.9, 2.2 Hz, 1H), 5.77 (dd, J = 9.0, 2.3 Hz, 1H), 4.32 (s, 2H), 3.22 (m, 2H), 1.32 ppm (t, J = 7.2 Hz, 3H). MS (ESI): 471.3 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.66 (s, 1H), 8.54 (s, 2H), 8.09 (s, 1H), 6.98 (s, 1H), 6.51 (m, 1H), 5.96 (m, 1H), 3.01 (s, 3H), 2.97 (m, 2H), 1.34 (m, 3H). MS (ESI): 456.3 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.55- 8.64 (m, 3H), 7.99 (d, J = 1.6 Hz, 1H), 6.85 (d, J = 2.4 Hz, 1H), 6.41 (dd, J = 11.9, 2.2 Hz, 1H), 5.77 (dd, J = 9.0, 2.3 Hz, 1H), 4.32 (s, 2H), 3.22 (m, 2H), 1.32 ppm (t, J = 7.2 Hz, 3H). MS (ESI): 471.3 ([M + H]+)
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.40 (s, 1H), 9.01 (s, 1H), 8.75 (s, 1H), 8.54 (d, 1H), 8.33 (m, 4H), 8.20 (s, 1H), 6.28 (m, 1H), 4.09 (m, 2H), 3.14 (d, 3H). MS (ESI): 499.1 ([M + H]+)
1H NMR (400 MHz, DMSO-d6): δ = 12.15 (s, 1H), 8.75 (s, 1H), 8.48 (s, 2H), 8.33 (s, 1H), 7.14 (d, J = 2.3 Hz, 1H), 6.44-6.54 (m, 1H), 5.88 (br s, 1H), 5.73 (dd, J = 9.0, 2.0 Hz, 1H), 3.22-3.30 (m, 2H), 2.62 (s, 3H), 1.32 ppm (t, J = 7.2 Hz, 3H). MS (ESI): 456.2 ([M + H]+).
1H NMR (400 MHz, DMOS-d6) δ ppm: 9.31 (s, 1H), 8.95 (d, J = 2.0 Hz, 1H), 8.76 (d, J = 2.0 Hz, 1H), 8.57 (s, 1H), 8.32 (t, J = 2.0 Hz, 1H), 8.10 (s, 1H), 6.45 (dd, J = 2.3, 12.3 Hz, 1H), 5.8-5.9 (m, 2H), 3.2-3.3 (m, 2H), 1.32 (t, J = 7.2 Hz, 3H). MS (ESI): 415.1 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ 8.6-8.7 (m, 1H), 8.3-8.4 (m, 1H), 8.13 (s, 1H), 7.6-7.7 (m, 1H), 7.16 (d, 1H, J = 2.3 Hz), 6.4- 6.5 (m, 1H), 5.8-5.9 (m, 1H), 5.6-5.7 (m, 1H), 5.3- 5.4 (m, 1H), 3.6-3.7 (m, 2H), 3.2-3.3 (m, 2H), 2.7- 2.8 (m, 2H), 1.9-2.1 (m, 2H), 1.6-1.7 (m, 1H), 1.4- 1.5 (m, 1H), 1.3-1.3 (m, 3H). MS (ESI): 526.4 ([M + H]+).
1H NMR (400 MHz, MeOH-d4) δ 8.5-8.5 (m, 1H), 8.3-8.3 (m, 2H), 8.0- 8.0 (m, 1H), 7.2-7.3 (m, 1H), 6.8-6.9 (m, 1H), 6.4- 6.4 (m, 1H), 5.8-5.8 (m, 1H), 5.2-5.3 (m, 1H), 3.3- 3.3 (m, 1H), 3.1-3.1 (m, 1H), 3.0-3.0 (m, 1H), 2.1- 2.2 (m, 2H), 1.9-2.0 (m, 3H), 1.7-1.9 (m, 1H), 1.3- 1.3 (m, 3H). MS (ESI): 541.3 ([M + H]+).
1H NMR (400 MHz, MeOH-d4) δ 8.5-8.5 (m, 1H), 8.5-8.5 (m, 2H), 7.9- 8.0 (m, 1H), 6.8-6.9 (m, 1H), 6.3-6.4 (m, 1H), 5.8- 5.9 (m, 1H), 4.8-4.9 (m, 1H), 3.18 (m, 2H), 1.41 (d, 3H, J = 6.6 Hz), 1.3-1.4 (m, 3H). MS (ESI): 486.3 ([M + H]+).
1H NMR (400 MHz, MeOH-d4) δ 8.63 (s, 1H), 8.41 (s, 2H), 8.08 (d, 1H, J = 1.6 Hz), 6.98 (d, 1H, J = 2.3 Hz), 6.51 (dd, 1H, J = 2.2, 11.9 Hz), 5.92 (dd, 1H, J = 2.3, 9.0 Hz), 5.6-5.6 (m, 1H), 3.3-3.4 (m, 3H), 3.2-3.3 (m, 3H), 3.08 (br d, 1H, J = 8.2 Hz), 2.2-2.3 (m, 1H), 2.1-2.2 (m, 1H) 1.43 (t, 3H, J = 7.2 Hz). MS (ESI): 527.1 ([M + H]+).
1H NMR (400 MHz, MeOH-d4) δ 8.62 (s, 1H), 8.43 (s, 1H), 8.09 (d, 1H, J = 1.6 Hz), 6.98 (d, 1H, J = 2.4 Hz), 6.51 (dd, 1H, J = 2.3, 11.9 Hz), 5.91 (dd, 1H, J = 2.3, 9.0 Hz), 5.65 (br t, 1H, J = 4.8 Hz), 3.3-3.6 (m, 3H), 3.2-3.3 (m, 4H), 2.2- 2.4 (m, 2H), 1.43 (t, 3H, J = 7.2 Hz). MS (ESI): 527.1 ([M + H]+).
1H NMR (40 0MHz, MeOH-d4) δ 8.6-8.7 (m, 1H), 8.5-8.6 (m, 2H), 8.0- 8.1 (m, 1H), 6.9-7.0 (m, 1H), 6.4-6.5 (m, 1H), 5.9- 6.0 (m, 1H), 4.9-5.0 (m, 1H), 3.3-3.3 (m, 2H), 1.5- 1.5 (m, 3H), 1.4-1.4 (m, 3H). MS (ESI): 486.2 ([M + H]+).
1H NMR (400 MHz, MeOH-d4) δ ppm: 8.51 (s, 1H), 8.40 (s, 1H), 8.21 (s, 1H), 7.85 (s, 1H), 7.55 (s, 1H), 6.77 (d, 1H), 6.35-6.39 (d, 1H), 5.79-5.82 (d, 1H), 4.15 (s, 1H), 3.21 (m, 2H), 2.41 (s, 6H), 1.28-1.32 (m, 3H). MS (ESI): 537.3 ([M + H]+)
1H NMR (400 MHz, MeOH-d4) δ 9.0-9.0 (m, 1H), 8.6-8.7 (m, 1H), 8.43 (s, 1H), 8.36 (d, 1H, J = 2.1 Hz), 8.0-8.1 (m, 1H), 6.7- 6.8 (m, 1H), 6.4-6.5 (m, 1H), 5.9-5.9 (m, 1H), 2.9- 3.0 (m, 2H), 1.3-1.4 (m, 3H). MS (ESI): 505.2 ([M + H]+).
1H NMR (400 MHz, MeOH-d4) δ 8.9-9.0 (m, 1H), 8.7-8.8 (m, 1H), 8.2- 8.3 (m, 2H), 8.15 (s, 1H), 6.74 (dd, 1H, J = 0.8, 2.4 Hz), 6.53 (dd, 1H, J = 2.3, 12.1 Hz), 6.03 (dd, 1H, J = 2.3, 8.9 Hz), 4.04 (s, 2H), 3.3-3.4 (m, 2H), 1.43 (t, 3H, J = 7.2 Hz). MS (ESI): 510.1 ([M + H]+).
A mixture of tert-butyl (3,4-dichloro-6-fluoro-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (1.1 g, 2.77 mmol, as the “CORE” in table 2), 3-(trifluoromethyl)-1H-pyrazole (753.6 mg, 5.54 mmol, as the “NUCLEOPHILE” in table 2) and K2CO3 (1.1 g, 8.31 mmol) in DMSO (15 mL) was stirred at 110° C. for 12 h. After cooled back to r.t., the mixture was poured into water (100 mL), and extracted with EtOAc (100 mL) three times. The combined organics were dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give the crude product, which was purified by column chromatography on silica gel eluted with (petroleum ether:EtOAc=5:1, v/v) to give the tert-butyl (3-chloro-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (1.2 g, 85.7% yield) as a white solid, MS (ESI): 498.1 ([{35Cl}M+H]+).
A solution of tert-butyl (3-chloro-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (100 mg, 0.2 mmol), (2-(1-(ethoxycarbonyl)cyclopropyl)pyrimidin-5-yl)boronic acid (94.8 mg, 0.4 mmol, as the “BORONIC REAGENT” in table 2) and K3PO4 (127.8 mg, 0.6 mmol) in THF (4 mL) and H2O (0.4 mL) was degassed with N2 for five times before Pd-Ad2nBuP Biphenyl (13.3 mg, 0.02 mmol, as the “CATALYST” in table 2) was added to the mixture at 20° C. under N2. After the reaction solution was degassed with N2 for five times again, it was stirred at 60° C. for 12 h under N2. The mixture was cooled back to r.t., poured into water (100 mL), and extracted with EtOAc (100 mL) three times. The combined organics were dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give the crude product, which was purified by Prep-TLC (petroleum ether:EtOAc=2:1) to give ethyl 1-(5-(8-((tert-butoxycarbonyl)(ethyl)amino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyrimidin-2-yl)cyclopropanecarboxylate (132.3 mg, 50.3% yield) as a yellow solid, MS (ESI): 654.2 ([M+H]+).
A solution of ethyl 1-(5-(8-((tert-butoxycarbonyl)(ethyl)amino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyrimidin-2-yl)cyclopropanecarboxylate (100 mg, 0.153 mmol) and NaOH (61.3 mg, 1.53 mmol) in EtOH (3 mL) and H2O (1 mL) was stirred at 20° C. for 2 h. Then the mixture was poured into water (50.0 mL), and adjusted to PH=3 with aq. HCl solution (2N), and extracted with DCM (100 mL) three times. The combined organics were dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give the crude product, which was used in the next step directly (92.3 mg, 96.4% yield). MS (ESI): 626.1 ([M+H]+).
To the solution of 1-(5-(8-((tert-butoxycarbonyl)(ethyl)amino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyrimidin-2-yl)cyclopropanecarboxylic acid (0.08 mmol) in DCM (3 mL) was added TFA (2 mL), and the solution was stirred at room temperature for 2 h before it was concentrated in vacuo. The residue was purified by prep-HPLC to give 1-[5-[8-(ethylamino)-6-fluoro-4-[3-(trifluoromethyl)pyrazol-1-yl]-9H-pyrido[2,3-b]indol-3-yl]pyrimidin-2-yl]cyclopropanecarboxylic acid (30 mg, 75% yield) as a yellow solid. 1H NMR (MeOH-d4, 400 MHz) δ 8.68 (s, 1H), 8.58 (s, 2H), 8.1-8.1 (m, 1H), 6.99 (d, 1H, J=2.3 Hz), 6.52 (dd, 1H, J=2.1, 12.0 Hz), 5.98 (dd, 1H, J=2.2, 9.0 Hz), 3.3-3.4 (m, 2H), 1.8-1.9 (m, 2H), 1.7-1.8 (m, 2H), 1.43 (t, 3H, J=7.2 Hz). MS: 526.1 ([M+H]+).
The following examples were prepared in analogy to Example 2.01, replacing tert-butyl N-(3,4-dichloro-6-fluoro-9H-pyrido[2,3-b]indol-8-yl)-N-ethyl-carbamate (Intermediate A2) as the “CORE” in step (a), 3-(trifluoromethyl)pyrazole as the “NUCLEOPHILE” in step (a), (2-(1-(ethoxycarbonyl)cyclopropyl)pyrimidin-5-yl)boronic acid as the “BORONIC REAGENT” in step (b), and Pd-Ad2nBuP Biphenyl as the “CATALYST” in step (b) with the reagents indicated in Table 2 respectively.
1H NMR and MS (ESI)
1H NMR (400 MHz, MeOH-d4) δ 8.68 (s, 1H), 8.58 (s, 2H), 8.12 (s, 1H), 6.99 (d, 1H, J = 2.0 Hz), 6.51 (dd, 1H, J = 2.1, 11.8 Hz), 5.98 (dd, 1H, J = 2.3, 9.0 Hz), 3.01 (s, 3H), 1.85 (m, 2H), 1.77 (m, 2H). MS (ESI): 512.1 ([M + H]+).
1H NMR (400 MHz, MeOH-d4) δ 8.63 (s, 1H), 8.39 (s, 2H), 8.05 (d, 1H, J = 1.5 Hz), 6.98 (d, 1H, J = 2.5 Hz), 6.50 (dd, 1H, J = 2.3, 12.0 Hz), 5.91 (dd, 1H, J = 2.3, 9.0 Hz), 4.43 (s, 2H), 3.3-3.4 (m, 1H), 3.29 (m, 2H), 1.43 (t, 3H, J = 7.0 Hz), 1.34 (s, 6H). MS (ESI): 558.1 ([M + H]+).
1H NMR (400 MHz, MeOH-d4) δ 8.57 (s, 1H), 8.09 (s, 2H), 8.02 (d, 1H, J = 1.5 Hz), 6.98 (d, 1H, J = 2.3 Hz), 6.49 (dd, 1H, J = 2.3, 12.0 Hz), 5.87 (dd, 1H, J = 2.3, 9.0 Hz), 3.66 (s, 2H), 3.3-3.3 (m, 2H), 1.42 (t, 3H, J = 7.0 Hz), 1.2-1.2 (m, 2H), 1.0-1.0 (m, 2H). MS (ESI): 555.1 ([M + H]+).
1H NMR (400 MHz, MeOH-d4) δ 8.57 (s, 1H), 8.11 (s, 2H), 8.0-8.0 (m, 1H), 6.97 (d, 1H, J = 2.4 Hz), 6.49 (dd, 1H, J = 2.3, 11.9 Hz), 5.88 (dd, 1H, J = 2.3, 9.0 Hz), 3.63 (s, 2H), 3.3- 3.3 (m, 2H), 1.42 (t, 3H, J = 7.2 Hz), 1.23 (s, 6H). MS (ESI): 557.1 ([M + H]+).
A solution of 1-(5-(8-((tert-butoxycarbonyl)(ethyl)amino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyrimidin-2-yl)cyclopropanecarboxylic acid (40 mg, 0.06 mmol, Example 2.01/step c), methanamine hydrochloride (21.4 mg, 0.32 mmol, as the “AMINE” in table 3), HATU (29.2 mg, 0.08 mmol) and DIPEA (24.7 mg, 0.19 mmol) in DMF (3 mL) was stirred at 20° C. for 12 h. After LC-MS showed the starting material was consumed, the mixture was poured into water (50 mL), and extracted with EtOAc (100 mL) three times. The combined organics were dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give the crude tert-butyl ethyl(6-fluoro-3-(2-(1-(methylcarbamoyl)cyclopropyl)pyrimidin-5-yl)-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-8-yl)carbamate (40 mg, 97.6% yield) as a yellow solid, which was used in the next step directly. MS (ESI): 639.3 ([M+H]+).
To a solution of tert-butyl ethyl(6-fluoro-3-(2-(1-(methylcarbamoyl)cyclopropyl)pyrimidin-5-yl)-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-8-yl)carbamate (30 mg, 0.05 mmol) in DCM (2 mL) was added TFA (1 mL), and the reaction mixture was stirred at 20° C. for 1 h. After LC-MS showed the starting material was consumed, the mixture was concentrated in vacuo to give the crude product, which was purified by Prep-HPLC (0.5% TFA in water) to give the 1-(5-(8-(ethylamino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyrimidin-2-yl)-N-methylcyclopropanecarboxamide (12 mg, 47.4% yield) as a yellow solid. MS (ESI): 539.2 ([M+H]+), 1H NMR (400 MHz, DMSO-d6): δ 12.18 (s, 1H), 8.92˜8.93 (d, J=4 Hz, 1H), 8.74 (s, 1H), 8.51 (s, 2H), 8.37˜8.75 (d, J=1.6 Hz, 1H), 7.16˜7.17 (d, J=2.4 Hz, 1H), 6.48˜6.52 (m, 1H), 5.91 (br, 1H), 5.76˜5.78 (m, 1H), 3.17˜3.27 (m, 2H), 2.71˜2.72 (m, 3H), 1.53˜1.54 (m, 2H), 1.41˜1.42 (m, 2H), 1.30˜1.34 (m, 3H).
The following examples were prepared in analogy to Example 3.01, replacing methyl amine as the “AMINE” in step (a) with the reagents indicated in Table 3.
1H NMR and MS (ESI)
1H NMR (400 MHz, DMSO-d6): δ 12.18 (s, 1H), 11.51 (s, 1H), 8.74 (s, 1H), 8.50 (s, 1H), 8.36 (s, 1H), 7.16 (s, 1H), 6.48~6.51 (m, 1H), 5.73~5.75 (m, 1H), 3.63 (s, 3H), 3.25~3.27 (m, 2H), 1.46 (m, 2H), 1.41 (m, 2H), 1.30~1.34 (m, 3H). MS (ESI): 555.3 ([M + H]+).
1H NMR (400 MHz, MeOH-d4) δ 8.7-8.8 (m, 1H), 8.5-8.6 (m, 1H), 8.4- 8.4 (m, 1H), 7.9-8.0 (m, 1H), 7.8-7.9 (m, 1H), 6.7- 6.8 (m, 1H), 6.3-6.4 (m, 1H), 5.8-5.9 (m, 1H), 3.7- 3.8 (m, 3H), 1.3-1.3 (m, 3H). MS (ESI): 514.1 ([M + H]+).
1H NMR (400 MHz, MeOH-d4) δ 8.7-8.8 (m, 3H), 8.0-8.1 (m, 1H), 6.9- 7.0 (m, 1H), 6.5-6.6 (m, 1H), 5.9-6.0 (m, 1H), 3.3- 3.3 (m, 2H), 1.3-1.5 (m, 3H). MS (ESI): 485.2 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.08 (s, 1 H) 8.68 (s, 1 H) 8.40 (s, 1 H) 8.24 (dd, J = 4.77, 1.71 Hz, 2 H) 7.44-7.55 (m, 2 H) 7.02-7.13 (m, 1 H) 6.96 (s, 1 H) 6.43-6.55 (m, 1 H) 5.80-5.95 (m, 1 H) 5.69 (dd, J = 9.17, 2.20 Hz, 1 H) 3.37 (s, 2 H) 3.26 (br dd, J = 6.97, 4.89 Hz, 2H) 1.28- 1.36 (m, 3 H). MS (ESI): 498.2 ([M + H]+)
1H NMR (400 MHz, DMSO-d6), δ ppm: 12.22 (s, 1H), 8.91 (d, J = 2.0 Hz, 1H), 8.38 (d, J = 2.0 Hz, 1H), 8.26 (s, 1H), 8.09 (s, 1H), 7.07 (d, J = 2.4 Hz, 1H), 6.49 (m, 1H), 5.95 (s, 1H), 5.76 (m, 1H), 3.47-3.42 (m, 4H), 3.27-3.22 (m, 5H), 1.32 (t, J = 7.2 Hz, 3H). MS (ESI): 542.1 ([M + H]+).
1H NMR (400 MHz, DMSO-d6) δ ppm: 12.16 (s, 1H), 8.93 (s, 1H), 8.89 (d, J = 2.0 Hz, 1H), 8.77 (s, 1H), 8.36 (d, J = 2.0 Hz, 1H), 8.24 (d, J = 2.0 Hz, 1H), 8.07 (t, J = 2.0 Hz, 1H), 7.06 (d, J = 2.0 Hz, 1H), 6.49 (m, 1H), 5.91 (brs, 1H), 5.76 (m, 1H), 3.47 (s, 2H), 3.28- 3.25 (m, 5H), 1.32 (t, J = 7.2 Hz, 3H), 0.81-0.75 (m, 4H). MS (ESI): 568.1 ([M + H]+).
The titled compound was synthesized according to the following scheme:
To a solution of tert-butyl (3,4-dichloro-6-fluoro-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (440 mg, 1.1 mmol, Intermediate A2) and K2CO3 (610 mg, 4.42 mmol) in DMSO (6 mL) was added ethyl 3-(trifluoromethyl)-1H-pyrazole-4-carboxylate (920 mg, 4.42 mmol, compound 4.01a), the reaction solution was stirred at 120° C. for 12 h. After cooled back to r.t., the mixture was poured into water and extracted by EtOAc. The organic layer was washed by brine, dried with anhy. Na2SO4, filtered, and concentrated in vacuo to give the crude product, which was purified by Prep-TLC and further purified by silica gel flash chromatography (TFA as additive) to give 1-(8-((tert-butoxycarbonyl)(ethyl)amino)-3-chloro-6-fluoro-9H-pyrido[2,3-b]indol-4-yl)-3-(trifluoromethyl)-1H-pyrazole-4-carboxylic acid (200 mg, 33.4% yield). MS (ESI): 542.2 ({35Cl}M+H)+, 544.2 ({37Cl}M+H)+, 564.2 ({35Cl}M+Na)+.
A solution of 1-(8-((tert-butoxycarbonyl)(ethyl)amino)-3-chloro-6-fluoro-9H-pyrido[2,3-b]indol-4-yl)-3-(trifluoromethyl)-1H-pyrazole-4-carboxylic acid (compound 4.01b) (200 mg, 0.369 mmol), 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)nicotinonitrile (compound 4.01c) (170 mg, 0.738 mmol) and CsF (224 mg, 1.476 mmol) in dioxane/H2O (6.0 mL, v/v=10:1) was degassed with N2 for five times, and then Pd2(dba)3 (34 mg, 0.037 mmol) and X-phos (36 mg, 0.074 mmol) were added into the mixture at 0° C. under N2. The reaction solution was degassed with N2 for five times again, and then stirred for 12 h at 110° C. for 12 h under N2. After cooled back to r.t., the mixture was poured into water and extracted by EtOAc. The organic layer was washed by brine and dried with anhy. Na2SO4 and filtered. The organic layer was concentrated in vacuo to give the crude product, which was purified by silica gel flash chromatography (TFA as additive) to give 1-(8-((tert-butoxycarbonyl)(ethyl)amino)-3-(5-cyanopyridin-3-yl)-6-fluoro-9H-pyrido[2,3-b]indol-4-yl)-3-(trifluoromethyl)-1H-pyrazole-4-carboxylic acid (200 mg, 88.9% yield) as a yellow solid. MS (ESI): 610.2 (M+H)+, 632.1 (M+Na)+.
To a solution of 1-(8-((tert-butoxycarbonyl)(ethyl)amino)-3-(5-cyanopyridin-3-yl)-6-fluoro-9H-pyrido[2,3-b]indol-4-yl)-3-(trifluoromethyl)-1H-pyrazole-4-carboxylic acid_(100 mg, 0.16 mmol, compound 4.01d), NH4Cl (43 mg, 0.8 mmol), HOBt (65 mg, 0.48 mmol), and PyBOP (250 mg, 0.48 mmol) in DMF (5.0 mL) was added DIPEA (124 mg, 0.96 mmol) under N2. The reaction mixture was stirred at 25° C. for 1 h, then purified by silica gel flash chromatography (TFA as additive) to give tert-butyl (4-(4-carbamoyl-3-(trifluoromethyl)-1H-pyrazol-1-yl)-3-(5-cyanopyridin-3-yl)-6-fluoro-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (90 mg, 90.2% yield) as a yellow solid. MS (ESI): 609.3 (M+H)+, 631.2 (M+Na)+.
To a solution of tert-butyl (4-(4-carbamoyl-3-(trifluoromethyl)-1H-pyrazol-1-yl)-3-(5-cyanopyridin-3-yl)-6-fluoro-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (80.0 mg, 0.131 mmol, compound 4.01e) in DCM (3 mL) was added TFA (1.5 mL), and the resulting mixture was stirred at 25° C. for 1 h. The reaction mixture was concentrated in vacuo to give a crude product, which was purified by Prep-HPLC (0.5% TFA in water) to give 1-(3-(5-cyanopyridin-3-yl)-8-(ethylamino)-6-fluoro-9H-pyrido[2,3-b]indol-4-yl)-3-(trifluoromethyl)-1H-pyrazole-4-carboxamide. (50 mg, 74.8% yield) as a yellow solid. 1H NMR (400 MHz, DMSO-d6) δ:ppm 12.23 (s, 1H) 9.01 (d, J=1.76 Hz, 1H), 8.77 (s, 1H), 8.56˜8.69 (m, 2H), 8.24 (t, J=1.88 Hz, 1H), 7.77 (br. s., 1H), 7.42 (br. s., 1H), 6.53 (dd, J=12.17, 1.88 Hz, 1H), 5.79˜6.00 (m, 2H), 3.27 (q, J=7.03 Hz, 2H), 1.33 (t, J=7.15 Hz, 3H). MS (ESI): 509.2 (M+H)+.
The titled compound was synthesized according to the following scheme:
To a solution of tert-butyl (3,4-dichloro-6-fluoro-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (440 mg, 1.1 mmol, Intermediate A2) and K2CO3 (610 mg, 4.42 mmol) in DMSO (6 mL) was added ethyl 3-(trifluoromethyl)-1H-pyrazole-4-carboxylate (920 mg, 4.42 mmol, compound 4.01a), and the reaction solution was stirred at 120° C. for 12 h. After cooled back to r.t., the mixture was poured into water and extracted by EtOAc (100 mL) two times. The combined organics were washed by brine, dried with anhy. Na2SO4, filtered, and concentrated in vacuo to give the crude product, which was purified by Prep-TLC and further purified by silica gel flash chromatography (TFA as additive) to give ethyl 1-(8-((tert-butoxycarbonyl)(ethyl)amino)-3-chloro-6-fluoro-9H-pyrido[2,3-b]indol-4-yl)-3-(trifluoromethyl)-1H-pyrazole-4-carboxylate (330 mg, 52.4% yield). MS (ESI): 570.1 ({35Cl}M+H)+, 572.2 ({37Cl}M+H)+.
To a solution of ethyl 1-(8-((tert-butoxycarbonyl)(ethyl)amino)-3-chloro-6-fluoro-9H-pyrido[2,3-b]indol-4-yl)-3-(trifluoromethyl)-1H-pyrazole-4-carboxylate (180 mg, 0.316 mmol, compound 5.01a) in THF (0.316 mL) was added LAH (30 mg, 0.79 mmol) at 0° C. under N2, and the resulting mixture was stirred at 0° C. for 1 h. The mixture was then poured into water and extracted by EtOAc (100 mL) two times. The combined organics were washed by brine, dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give tert-butyl (3-chloro-6-fluoro-4-(4-(hydroxymethyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (140 mg, 84% yield) as a yellow solid. It was used in the next step directly without purification. MS (ESI): 528.1 ({35Cl}M+H)+, 530.1 ({37Cl}M+H)+.
A solution of tert-butyl (3-chloro-6-fluoro-4-(4-(hydroxymethyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (130 mg, 0.246 mmol, compound 5.01b), 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)nicotinonitrile (113 mg, 0.493 mmol), and CsF (150 mg, 0.986 mmol) in dioxane/H2O (4 mL, v/v=10:1) was degassed with N2 for five times, and then Pd2(dba)3 (22.5 mg, 0.0246 mmol) and XPhos (23.5 mg, 0.0493 mmol) was added into the mixture at 0° C. under N2. The reaction solution was degassed with N2 for five times again, and the mixture was stirred at 110° C. for 12 h. After cooled back to r.t., the mixture was poured into water and extracted by EtOAc (100 mL) two times. The combined organics were washed by brine, dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give the crude product, which was purified by Prep-TLC to give tert-butyl (3-(5-cyanopyridin-3-yl)-6-fluoro-4-(4-(hydroxymethyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (120 mg, yield: 81.8% yield) as a pale yellow solid. MS (ESI): 596.3 (M+H)+, 618.2 (M+Na)+.
To a solution of tert-butyl (3-(5-cyanopyridin-3-yl)-6-fluoro-4-(4-(hydroxymethyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (110 mg, 0.185 mmol, compound 5.01c) in DCM (3 mL) was added TFA (1.5 mL). The reaction mixture was stirred at 28° C. for 1 h, then concentrated in vacuo to give the crude product, which was purified by Prep-HPLC (0.5% TFA in water) to give 5-(8-(ethylamino)-6-fluoro-4-(4-(hydroxymethyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)nicotinonitrile (77 mg, 84.2% yield) as a yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 12.16 (s, 1H), 8.99 (d, J=1.25 Hz, 1H), 8.74 (s, 1H), 8.59 (d, J=1.76 Hz, 1H), 8.08˜8.24 (m, 2H), 6.51 (d, J=12.30 Hz, 1H), 5.92 (d, J=9.03 Hz, 1H), 4.51 (s, 4H), 3.26 (q, J=6.94 Hz, 2H), 1.32 (t, J=7.03 Hz, 3H). MS (ESI): 496.2 (M+H)+, 248.6 (M/2+H)+, 518.2 (M+Na)+.
The titled compound was synthesized according to the following scheme:
To a mixture of tert-butyl (3-chloro-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (100 mg, 0.2 mmol), ethyl 2-(5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-3-yl)acetate (83.56 mg, 0.4 mmol) in THF (2 mL) and water (0.2 mL) was added K3PO4 (127.91 mg, 0.6 mmol) and cataCXium® A Pd G2 (13.43 mg, 0.02 mmol, Sigma-Aldrich, Catalog #: 761311). the mixture was stirred at 80° C. for 16 h under argon. After cooled back to r.t., the mixture was concentrated in vacuo to give the crude product, which was purified by Prep-TLC (petroleum ether/EtOAc=2/1) to give ethyl 2-(5-(8-((tert-butoxycarbonyl)(ethyl)amino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyridin-3-yl)acetate (100 mg, 79.5% yield) as a brown solid. MS (ESI): 627.2 [(M+H)+].
To a solution of ethyl 2-(5-(8-((tert-butoxycarbonyl)(ethyl)amino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyridin-3-yl)acetate (100 mg, 0.160 mmol) in EtOH (5 mL) was added aq. NaOH solution (1 mL, 1 N, 1 mmol). After been stirred at 20° C. for 1 h, the mixture was adjusted to pH=4-5 with aq. HCl solution (1N) and then extracted with EtOAc (20 mL) three times. The combined organics were dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give 2-(5-(8-((tert-butoxycarbonyl)(ethyl)amino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyridin-3-yl)acetic acid (90 mg, 94.2% yield) as a yellow solid. MS (ESI): 599.0 [(M+H)+].
A solution of 2-(5-(8-((tert-butoxycarbonyl)(ethyl)amino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyridin-3-yl)acetic acid (90 mg, 0.15 mmol), HATU (68.61 mg, 0.180 mmol), and DIPEA (0.08 mL, 0.450 mmol) in DMF (3 mL) was stirred at 20° C. for 0.5 h, then tert-Butyl carbazate (23.85 mg, 0.180 mmol) was added and the resulting mixture was stirred at 20° C. for another 16 h. EtOAc (30 mL) was added and the mixture was washed with brine (20 mL), satd. aq. NaHCO3 solution (20 mL). The organic layer was dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give tert-butyl 2-(2-(5-(8-((tert-butoxycarbonyl)(ethyl)amino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyridin-3-yl)acetyl)hydrazine-carboxylate (100 mg, 93.3% yield) as a yellow solid. MS (ESI): 713.2 [(M+H)+].
To a mixture of tert-butyl 2-(2-(5-(8-((tert-butoxycarbonyl)(ethyl)amino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyridin-3-yl)acetyl)hydrazine-carboxylate (100 mg, 0.14 mmol) in EtOAc (5 mL) was added HCl solution in EtOAc (1.0 mL, 4N, 4 mmol). The mixture was stirred at 20° C. for 1 h before it was concentrated in vacuo to give a crude product of 2-(5-(8-(ethylamino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyridin-3-yl)acetohydrazide (100 mg, 65.2% yield) as a yellow solid. MS (ESI): 513.1 [(M+H)+].
To a solution of 2-(5-(8-(ethylamino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyridin-3-yl)acetohydrazide (100 mg, 0.2 mmol) in THF (5 mL) was added DIPEA (0.1 mL, 0.590 mmol), then the mixture was stirred at 20° C. for 0.5 h. Then CDI (63 mg, 0.39 mmol) was added and the mixture was stirred at 20° C. for another 16 h. EtOAc (20 mL) was added and the mixture was washed with brine (20 mL), satd. NH4Cl solution (20 mL) two times. The organic layer was dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give the crude product, which was purified by Prep-HPLC (FA) to give 5-((5-(8-(ethylamino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyridin-3-yl)methyl)-1,3,4-oxadiazol-2(3H)-one (19.2 mg, 18.3% yield) as a yellow solid. MS (ESI): 539.1 [(M+H)+]. 1H NMR (400 MHz, DMSO-d6) δ ppm: 12.22 (brs, 1H) 8.71 (s, 1H) 8.49 (d, J=1.6 Hz, 1H) 8.44 (brs, 1H) 8.36 (d, J=1.6 Hz, 1H) 8.25 (d, J=1.2 Hz, 1H) 7.48 (s, 1H) 7.02 (d, J=1.2 Hz, 1H) 6.48 (dd, J=12.0, 2.0 Hz, 1H) 5.96 (brs, 1H) 5.71 (dd, J=12.0, 2.0 Hz, 1H) 3.93 (s, 2H) 3.18-3.28 (m, 2H) 1.32 (t, J=7.2 Hz, 3H).
The titled compound was synthesized according to the following scheme:
To a solution of ethyl 2-(5-(8-((tert-butoxycarbonyl)(ethyl)amino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyridin-3-yl)acetate (50 mg, 0.08 mmol) in EtOAc (2 mL) was added HCl solution in EtOAc (1.0 mL, 4N, 4 mmol), and the mixture was stirred at 20° C. for 2 h before concentrated in vacuo to give a crude product of ethyl 2-(5-(8-(ethylamino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyridin-3-yl)acetate (40 mg) as a yellow solid.
To a solution of ethyl 2-(5-(8-(ethylamino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyridin-3-yl)acetate (40 mg, 0.08 mmol) in THF (2 mL) was added LAH (2.88 mg, 0.08 mmol) at 20° C., then the mixture was stirred at 20° C. for 16 h. Water (10 mL) was added to quench the reaction and the mixture was extracted with EtOAc (10 mL) three times. The combined organics were dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give the crude material, which was purified by Prep-HPLC (FA) to give 5-((5-(8-(ethylamino)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-3-yl)pyridin-3-yl)methyl)-1,3,4-oxadiazol-2(3H)-one (8.1 mg, 21.2% yield) as a yellow solid. MS (ESI): 485.2 [(M+H)+]. 1H NMR (400 MHz, DMSO) δ ppm: 12.17 (brs, 1H) 8.71 (s, 1H) 8.43 (brs, 1H) 8.38 (s, 1H) 8.29 (s, 1H) 8.21 (s, 1H) 7.35 (s, 1H) 7.07 (s, 1H) 6.48 (m, 1H) 5.96 (brs, 1H) 5.70 (m, 1H) 4.71 (brs, 1H) 3.53 (m, 1H) 3.26 (m, 1H) 2.62-2.72 (m, 2H) 1.32 (m, 3H).
The titled compound was synthesized according to the following scheme:
To a solution of tert-butyl (3-chloro-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (250 mg, 0.5 mmol) and (5-(cyanomethyl)pyridin-3-yl)boronic acid (110 mg, 0.68 mmol) in THF/H2O (10 mL/1 mL) was added cataCXium® A Pd G2 (40 mg, 0.06 mmol, Sigma-Aldrich, Catalog #: 761311) and K3PO4 (280 mg, 2.63 mmol), then the solution was stirred at 80° C. for 18 h under Ar. After cooled back to r.t., the reaction mixture was concentrated in vacuo to give a crude product, which was purified by Prep-TLC (petroleum ether/EtOAc=%) to give tert-butyl (3-(5-(cyanomethyl)pyridin-3-yl)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (260.0 mg, 89.3% yield) as a yellow solid. MS (ESI): 580.1 [(M+H)+].
To a solution of tert-butyl (3-(5-(cyanomethyl)pyridin-3-yl)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (80 mg, 0.138 mmol) in MeOH (5 mL) was added NaOMe (10 mg, 0.185 mmol). After the reaction mixture was stirred at 20° C. for 120 h under N2, NH4Cl (30 mg, 0.56 mmol) was added and then the resulting solution was stirred at 20° C. for additional 20 h. Then the reaction mixture was concentrated in vacuo to give a crude product, which was re-dissolved in EtOAc (50 mL) and filtered. The filtrate was then concentrated in vacuo to give a crude product of tert-butyl (3-(5-(2-amino-2-iminoethyl)pyridin-3-yl)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (76.0 mg) as a white solid. It was used in the next step without further purification. MS (ESI): 597.2 [(M+H)+].
To a solution of tert-butyl (3-(5-(2-amino-2-iminoethyl)pyridin-3-yl)-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-8-yl)(ethyl)carbamate (76 mg, 0.051 mmol) in MeOH (5 mL) was added 2,2-dimethoxyethanamine (15 mg, 0.143 mmol). After been stirred at 70° C. for 18 h, the reaction mixture was concentrated in vacuo to give a crude solid, which was dissolved in aq. oxalic acid solution (1N, 5 mL) and the resulting mixture was stirred at 80° C. for another 3 h. The reaction mixture was cooled back to r.t., diluted with H2O (30 mL), and basified with satd. aq. Na2CO3 solution to pH ˜9. The aqueous solution was extracted with EtOAc (30 mL) three times, and the combined organics were dried over anhy. Na2SO4, filtered, and concentrated in vacuo to give a crude product. The crude product was then purified by Prep-HPLC to give 3-(5-((1H-imidazol-2-yl)methyl)pyridin-3-yl)-N-ethyl-6-fluoro-4-(3-(trifluoromethyl)-1H-pyrazol-1-yl)-9H-pyrido[2,3-b]indol-8-amine (13.2 mg, 49.8% yield) as a light-yellow solid. MS (ESI): 521.1 [(M+H)+]. 1H NMR (400 MHz, DMSO-d6) δ ppm: 12.20 (brs, 1H), 8.65 (s, 1H), 8.41 (s, 1H), 8.30 (s, 1H), 8.26 (s, 0.23H), 8.19 (m, 2H), 7.50 (s, 1H), 7.02 (s, 1H), 6.89 (s, 2H), 6.48-6.45 (d, J=11.6 Hz, 1H), 5.95 (s, 1H), 5.69-5.67 (m, 1H), 3.95 (s, 2H), 3.27-3.22 (q, J=7.2 Hz, 2H), 1.32-1.29 (t, J=7.2 Hz, 2H).
The in vitro antimicrobial activity of the compounds against E. coli (BW25113, obtained from Coli Genetic Stock Center, #7636) and K. pneumoniae (ATCC10031) was determined according to the following procedure:
The assay used a 10-points Iso-Sensitest broth medium to measure quantitatively the in vitro activity of the compounds against E. coli BW25113 and K. pneumoniae ATCC 10031.
Stock compounds in DMSO were serially two-fold diluted (range from 50 to 0.097 μM final concentration) in 384 wells microtiter plates and inoculated with 49 μL the bacterial suspension in Iso-Sensitest broth medium to have a final cell concentration of ˜5×105 CFU/mL in a final volume/well of 50 μL/well. Microtiter plates were incubated at 35±2° C.
Bacterial cell growth was determined with the measurement of optical density at λ=600 nm each 20 minutes over a time course of 16 h.
Growth inhibition was calculated during the logarithmic growth of the bacterial cells with determination of the concentration inhibiting 50% (IC50) of the growth.
GP-6 (Example 4.131 disclosed in WO 2012/125746 A1) was used as the reference compound in Table 5-10.
Compounds of the present invention were tested for their concentration inhibiting 50% (IC50). The data of IC50 over E. coli (BW25113) and K. pneumoniae (ATCC10031) are illustrated in Table 4. Particular compounds of the present invention were found to have IC50≤1 μM.
E. coli K12
K. pneumoniae
The in vitro potency of compounds to inhibit E. coli (ATCC 25922) growth was assessed by MIC (Minimal Inhibitory Concentration) assay. Samples were prepared from 10 mM DMSO stock solutions. After serial 2-fold dilution in DMSO in a master plate (Greiner, Cat No: 651201), 180 μL sterile distilled water was added to 20 μL each aliquot of the samples. Then 10 μL diluted compounds were transferred into a new assay plate (Costar, 3599).
The growth medium Caution-Adjusted Mueller Hinton Broth (CAMHB) was prepared by adding the 20 mg per liter CaCl2 and 20 mg per liter MgCl2 into the MHB medium (Jianglai Company, sterilized).
Vials of each of the test microorganisms were maintained frozen in the vapor phase of a liquid nitrogen freezer. Took out the bacteria E. coli ATCC 25922 (KWIKSTIK, 0335K) from liquid nitrogen freezer, thawed it in room temperature, and diluted the bacterial in the CAMHB medium to achieve a final inoculum of 5×105 CFU/mL, 90 μL CAMHB with bacteria was dispensed to the assay plate and pipetted 5 times.
Then the assay plates were incubated for 20 hours at 35° C. in ambient air. Following incubation, the MIC (μg/mL), the lowest concentration of drug that inhibits visible growth of the microorganism was read and recorded throw the microscope.
E. coli ATCC 25922
The solubility of compounds of present invention was assessed by LYSA assay. Samples were prepared in duplicate from 10 mM DMSO stock solutions (20 μL) diluted in 30 μL pure DMSO. After evaporation of DMSO with a centrifugal vacuum evaporator, the residue was dissolved in 0.05 M phosphate buffer (150 μL, pH 6.5), stirred for one hour and shook for two hours. After one night, the solution was filtered using a microtiter filter plate. Then the filtrate and its 1/10 dilution were analyzed by HPLC-UV. In addition a four-point calibration curve was prepared from the 10 mM stock solutions and used for the solubility determination of the compounds. The results were in μg/mL. and summarized in Table 6. Particular compounds of the present invention were found to have LYSA >10 μg/mL with much improved solubility compared to GP-6.
The cytotoxicity of compounds of present invention was assessed by HepG2 assay. HepG2 cells (ATCC HB-8065™) were seeded into 96-well plates (1.2×104 cells per well) and treated with compounds for 3 days, cell viability was measured at day 3 post compound treatment using the CellTiter-Blue® Cell Viability Assay (Promega, Cat. No. G8082). 20 μL of Cell titer blue buffer were added to each well of the cells, incubated for 3 hours at 37° C., and the fluorescence value was measured by a plate reader (Molecular Device SpectraMax M2). The CC50 value of each compound is plotted with GraphPad Prism using four parameter logistic equation. Results of cytotoxicity are given in Table 7.
| Number | Date | Country | Kind |
|---|---|---|---|
| PCT/CN2018/117721 | Nov 2018 | CN | national |
This application is a continuation of International Application No. PCT/EP2019/082336 having an International filing date of Nov. 25, 2019, which claims benefit of priority to International Application No. PCT/CN2018/117721 having an International filing date of Nov. 27, 2018, all of which are incorporated by reference in their entirety.
| Number | Date | Country | |
|---|---|---|---|
| Parent | PCT/EP2019/082336 | Nov 2019 | US |
| Child | 17331599 | US |
