Patent Grant
12012418
Aryl hydrocarbon receptor (AhR), a basic helix-loop-helix protein, is a member of the Per-ARNT-Sim (PAS) superfamily of proteins. Physiologically, many of these proteins act by sensing molecules and stimuli from the cellular/tissue microenvironment, thereby initiating signaling cascades necessary to elicit appropriate cellular responses.
In its inactive state, AhR resides in the cytosol bound to several co-chaperones, but after activation, it migrates into the nucleus and binds its dimerization partner, ARNT (another bHLH-PAS protein), thus initiating the transcription of a variety of genes with promoters containing a dioxin (DRE) or xenobiotic consensus sequence (XME). Pioneering studies in AhR-deficient mice have emphasized the role of AhR in the development and functions of various organs. More recent studies have shown that AhR controls specific immune responses (see, for example, Stockinger et al., Semin. Immunol. 2011, 23, 99-105).
AhR is highly expressed by T cells and controls Th1/Th2/Th17 cell-associated immunity. In humans and in mouse models of inflammatory bowel disease, activation of AhR led to a diminished expression of IFN-7 and T-bet, the main transcription factor that drives the Th1 polarization. The basic mechanism by which AhR inhibits IFN-γ production remains to be ascertained, however, it has been demonstrated that AhR signaling enhances Aiolos, a member of the Ikaros family, that negatively regulates IFN-γ production and colitis in mice. AhR activation in lymphoid cells can also regulate production of interleukin-22 (IL-22), a cytokine that can exert protective effects in various organs and time-course studies showed that suppression of IFN-γ and T-bet preceded IL-22 induction.
In light of the potential for selective AhR modulators to affect immunity and inflammation and thus treat a variety of inflammatory conditions, there exists a need for potent and selective compounds that modulate AhR activity.
The present disclosure, in an embodiment, provides potent and selective AhR-binding compounds. In one aspect, the present disclosure provides a compound according to Formula I:
wherein the variables are as defined below.
In one aspect, the present disclosure relates to a compound according to Formula I-A:
wherein the variables are as defined below.
In one aspect, the present disclosure relates to a compound according to Formula II:
wherein the variables are as defined below.
In one aspect, the present disclosure relates to a compound according to Formula III:
wherein the variables are as defined below.
In one aspect, the present disclosure relates to a compound according to Formula IV:
wherein the variables are as defined below.
The present disclosure also provides methods of treating an inflammatory disease or condition, comprising administering to a subject in need thereof a therapeutically effective amount of a compound described above. In certain embodiments, the inflammatory disease or condition is selected from the group consisting of inflammatory bowel disease, cartilage inflammation, bone degradation, ulcerative colitis, psoriasis, arthritis, psoriatic arthritis, rheumatoid arthritis, juvenile arthritis, juvenile rheumatoid arthritis, autoimmune hepatitis, Crohn's disease, lupus erythematosus, multiple sclerosis, Alzheimer's disease, dermatitis, atopic dermatitis, acne, Type I diabetes mellitus, Raynaud's phenomenon, Graves' disease, and Addison's disease.
In certain embodiments, the inflammatory disease or condition is selected from the group consisting of Crohn's disease, ulcerative colitis, collagenous colitis, lymphocytic colitis, diversion colitis, Behçet's disease, idiopathic inflammatory bowel disease, irritable bowel syndrome, regional enteritis, spastic colon, microscopic colitis, Crohn's colitis, perianal disease, indeterminate colitis, lymphocytic gastritis, and eosinophilic enteritis.
In certain embodiments, the inflammatory disease or condition is Crohn's disease. In certain embodiments, the Crohn's disease is selected from the group consisting of ileocolitis, ileitis, gastroduodenal Crohn's disease, jejunoileitis, and granulomatous ileocolitis. In certain embodiments, the Crohn's disease includes intestinal fibrosis. In certain embodiments, the Crohn's disease is fibrostenotic Crohn's disease.
In another aspect, the present disclosure provides a method of preventing, treating, or reducing fibrostenosis or intestinal fibrosis in a subject, comprising administering to a subject in need thereof a therapeutically effective amount of a compound disclosed herein. In certain embodiments, the fibrostenosis or intestinal fibrosis is associated with Crohn's disease.
In another aspect, the present invention provides a compound as described above for use as a medicament. The present invention further provides a compound as described above for use in a method of treating an inflammatory disease or condition. The method comprises administering to a subject in need thereof a therapeutically effective amount of said compound. In certain embodiments, the inflammatory disease or condition is selected from the group consisting of inflammatory bowel disease, cartilage inflammation, bone degradation, ulcerative colitis, psoriasis, arthritis, psoriatic arthritis, rheumatoid arthritis, juvenile arthritis, juvenile rheumatoid arthritis, autoimmune hepatitis, Crohn's disease, lupus erythematosus, multiple sclerosis, Alzheimer's disease, dermatitis, atopic dermatitis, acne, Type I diabetes mellitus, Raynaud's phenomenon, Graves' disease, and Addison's disease. In certain embodiments, the inflammatory disease or condition is selected from the group consisting of Crohn's disease, ulcerative colitis, collagenous colitis, lymphocytic colitis, diversion colitis, Behçet's disease, idiopathic inflammatory bowel disease, irritable bowel syndrome, regional enteritis, spastic colon, microscopic colitis, Crohn's colitis, perianal disease, indeterminate colitis, lymphocytic gastritis, and eosinophilic enteritis. In certain embodiments, the inflammatory disease or condition is Crohn's disease. In certain embodiments, the Crohn's disease is selected from the group consisting of ileocolitis, ileitis, gastroduodenal Crohn's disease, jejunoileitis, and granulomatous ileocolitis. In certain embodiments, the Crohn's disease includes intestinal fibrosis. In certain embodiments, the Crohn's disease is fibrostenotic Crohn's disease.
In yet another aspect, the present invention provides the compound as described above for use in a method of preventing, treating or reducing fibrostenosis or intestinal fibrosis in a subject. The method comprises administering to a subject in need thereof a therapeutically effective amount of said compound. In certain embodiments, the fibrostenosis or intestinal fibrosis is associated with Crohn's disease.
In one aspect, the present disclosure provides a compound according to Formula I:
or a pharmaceutically acceptable salt thereof, wherein:
A is
Ar is represented by
G1 is CR4 or N;
G2, G3, and G4 are each independently CR42 or NR1;
X1 is independently for each occurrence H, halogen, —OH, —CN, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C3-6 cycloalkyl, C1-6 alkoxy, —O-phenyl, —N(R1)2, —NO2, —C1-6 alkylene-N(R1)2, —C(O)N(R3)2, —CO2R3, —C(O)R3, —SR3, —SO2R3, —SO3R3, or —SO2N(R3)2;
X2 is independently for each occurrence H, halogen, —OH, —CN, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C3-6 cycloalkyl, C1-6 alkoxy, —O-phenyl, —N(R1)2, —NO2, —C1-6 alkylene-N(R1)2, —C(O)N(R3)2, —CO2R3, —SR3, —SO2R3, —SO3R3, or —SO2N(R3)2;
R1 is independently for each occurrence H or C1-6 alkyl;
R2 is independently for each occurrence H or C1-6 alkyl;
R3 is independently for each occurrence selected from the group consisting of H, C1-6 alkyl, phenyl, or heteroaryl;
R4 is independently for each occurrence selected from the group consisting of H, C1-6 alkyl, and halogen;
n is independently for each occurrence 0, 1, 2, or 3; and
m is independently for each occurrence 0, 1, 2, 3, or 4;
wherein each instance of C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C3-6 cycloalkyl, C1-6 alkoxy, or —O-phenyl may be optionally substituted with 1, 2, or 3 substituents each independently selected from the group consisting of halogen, —OH, —CN, —NR′R″—C(O)N(R′R″), and —C(O)R′ (wherein R′ and R″ are each independently selected from H, methyl, ethyl, propyl or butyl, or R′ and R″ taken together form a 4-6 membered heterocycle);
wherein when A is
G1 is CH, G3, and G4 are CH2, and G2 is NR1, X2 is not H (i.e., is not present, n is 0).
In certain embodiments, G1 is N.
In certain embodiments, G2 is NH.
In certain embodiments, G1 is CH, G2 is NH, and G3 and G4 are CH2.
In certain embodiments, G1 is N and G2, G3, and G4 are CH2.
In certain embodiments, G1 is N, G2 and G3 are CH2, and G4 is NH.
In certain embodiments, G1 is N, G2 and G4 are CH2, and G3 is NH.
In certain embodiments, G1 is CH, G2 and G4 are NH, and G3 is CH2.
In certain embodiments, Ar is
and m is 1 or 2.
In certain embodiments, Ar is
In certain embodiments, X1 is H, halogen, —CN, or —OMe.
In certain embodiments, X1 is —OMe.
In certain embodiments, X2 is selected from the group consisting of H, halogen, —OH, —CN, C1-6 alkoxy, and CF3.
In certain embodiments, X2 is CF3 and n is 1.
In certain embodiments, the compound is selected from the group consisting of
In one aspect, the present disclosure relates to a compound according to Formula I-A:
or a pharmaceutically acceptable salt thereof, wherein:
In certain embodiments, X2 is selected from the group consisting of H, —OMe, ethyl, —OH, and —OCH2Ph.
In certain embodiments, R5 is selected from the group consisting of H, acetyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl, isopentyl, and —CO2CH2Ph.
In certain embodiments, X1 is independently for each occurrence H, —OMe, —NO2, —CO2Me, methyl, —OC(O)Me, —OCH2Ph, —OH, —NH2, or tert-butyl; or two instances of X1 taken together may be —O(CH2)2O—.
In certain embodiments, n is 3.
In one aspect, the present disclosure relates to a compound according to Formula II:
or a pharmaceutically acceptable salt thereof, wherein:
B is
C is
Ar is represented by
X1 is independently for each occurrence, if present, selected from halogen, —OH, —CN, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C3-6 cycloalkyl, C1-6 alkoxy, —O-phenyl, —N(R1)2, —NO2, —C1-6 alkylene-N(R1)2, —C(O)N(R3)2, —CO2R3, —C(O)R3, —SR3, —SO2R3, —SO3R3, or —SO2N(R3)2;
X2 is independently for each occurrence if present, selected from, halogen, —OH, —CN, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C3-6 cycloalkyl, C1-6 alkoxy, —O-phenyl, —N(R1)2, —NO2, —C1-6 alkylene-N(R1)2, —C(O)N(R3)2, —CO2R3, —SR3, —SO2R3, —SO3R3, or —SO2N(R3)2;
R1 is independently for each occurrence H or C1-6 alkyl;
R3 is independently for each occurrence H, C1-6 alkyl, phenyl, or heteroaryl;
R4 is selected from the group consisting of H, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, —N(R1)2, —C1-6 alkylene-N(R1)2, —C(O)N(R3)2, and —CO2R3;
n is independently for each occurrence 0, 1, 2, or 3;
m is independently for each occurrence 0, 1, 2, 3, or 4;
wherein each instance of C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C3-6 cycloalkyl, C1-6 alkoxy, or —O-phenyl may be optionally substituted with 1, 2, or 3 substituents each independently selected from the group consisting of halogen, —OH, —CN, —NR′R″—C(O)N(R′R″), and —C(O)R′ (wherein R′ and R″ are each independently selected from H, methyl, ethyl, propyl or butyl, or R′ and R″ taken together form a 4-6 membered heterocycle);
X3 is N or CR4;
X4 is NR1, O, or S; and
Y is a bond, C1-6 alkylene or —N(R1)—;
wherein when B is
and X4 is S, X2 is not H (i.e., is not present).
In certain embodiments of Formula II Y is CH2 or NH.
In certain embodiments, R4 is selected from the group consisting of C2-6 alkenyl, C2-6 alkynyl, —C1-6 alkylene-N(R1)2, and —C(O)N(R3)2.
In certain embodiments, R4 is allyl or
In certain embodiments, Ar is
and m is 1 or 2.
In certain embodiments, Ar is
In certain embodiments, X1 is H (i.e., n is 0), halogen, —CN, or —OMe.
In certain embodiments, A, B and L taken together are compounds represented by:
In certain embodiments, X2 is selected from the group consisting of H, halogen, —OH, —CN, C1-6 alkoxy, and CF3.
In certain embodiments, X2 is CF3 and n is 1.
In one aspect, the present disclosure relates to a compound according to Formula III:
or a pharmaceutically acceptable salt thereof, wherein:
D is
Ar is represented by
X1 may be present, and may be selected independently for each occurrence from, halogen, —OH, —CN, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C3-6 cycloalkyl, C1-6 alkoxy, —O-phenyl, —N(R1)2, —NO2, —C1-6 alkylene-N(R1)2, —C(O)N(R3)2, —CO2R3, —C(O)R3, —SR3, —SO2R3, —SO3R3, or —SO2N(R3)2;
X2 may be present, and may be selected independently for each occurrence from, halogen, —OH, —CN, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C3-6 cycloalkyl, C1-6 alkoxy, —O-phenyl, —N(R1)2, —NO2, —C1-6 alkylene-N(R1)2, —C(O)N(R3)2, —CO2R3, —SR3, —SO2R3, —SO3R3, or —SO2N(R3)2;
R1 is independently for each occurrence H or C1-6 alkyl;
R3 is independently for each occurrence H, C1-6 alkyl, phenyl, or heteroaryl;
n is independently for each occurrence 0, 1, 2, or 3; and
m is independently for each occurrence 0, 1, 2, 3, or 4;
wherein each instance of C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C3-6 cycloalkyl, C1-6 alkoxy, or —O-phenyl may be optionally substituted with 1, 2, or 3 substituents each independently selected from the group consisting of halogen, —OH, —CN, —NR′R″—C(O)N(R′R″), and —C(O)R′ (wherein R′ and R″ are each independently selected from H, methyl, ethyl, propyl or butyl, or R′ and R″ taken together form a 4-6 membered heterocycle);
wherein when D is
X2 is not H (i.e., X2 is not present and n is 0).
In certain embodiments, Ar is
and m is 1 or 2.
In certain embodiments, Ar is
In certain embodiments, X1 is H, halogen, —CN, or —OMe.
In certain embodiments, X1 is —OMe.
In certain embodiments, X2 is selected from the group consisting of H, halogen, —OH, —CN, C1-6 alkoxy, and CF3.
In certain embodiments, X2 is CF3 and n is 1.
In one aspect, the present disclosure relates to a compound according to Formula IV:
or a pharmaceutically acceptable salt thereof, wherein:
E is a ring selected from the group consisting of heterocyclyl, 5-membered heteroaromatic, 6-membered heteroaromatic, aromatic, and C3-6 cycloalkyl, wherein the ring is optionally substituted with 1, 2, or 3 X2;
Ar is represented by
X1 is if present, selected independently for each occurrence from the group consisting of H, halogen, —OH, —CN, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C3-6 cycloalkyl, C1-6 alkoxy, —O-phenyl, —N(R1)2, —NO2, —C1-6 alkylene-N(R1)2, —C(O)N(R3)2, —CO2R3, —C(O)R3, —SR3, —SO2R3, —SO3R3, or —SO2N(R3)2;
X2 is independently for each occurrence selected from the group consisting of halogen, —OH, —CN, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C3-6 cycloalkyl, C1-6 alkoxy, —O-phenyl, —N(R1)2, —NO2, —C1-6 alkylene-N(R1)2, —C(O)N(R3)2, —CO2R3, —SR3, —SO2R3, —SO3R3, or —SO2N(R3)2; Y is selected from the group consisting of a bond, NR′ or C1-6alkylene (e.g., a bond or C1-2alkylene);
R1 is independently for each occurrence H or C1-6 alkyl;
R3 is independently for each occurrence selected from the group consisting of H, C1-6 alkyl, phenyl, or heteroaryl; and
m is independently for each occurrence 0, 1, 2, 3, or 4;
wherein each instance of C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C3-6 cycloalkyl, C1-6 alkoxy, or —O-phenyl may be optionally substituted with 1, 2, or 3 substituents each independently selected from the group consisting of halogen, —OH, —CN, —NR′R″—C(O)N(R′R″), and —C(O)R′ (wherein R′ and R″ are each independently selected from H, methyl, ethyl, propyl or butyl, or R′ and R″ taken together form a 4-6 membered heterocycle).
In certain embodiments, of Formula IV, Y is a bond, CH2 or NH; (for example Y may be a bond).
In certain embodiments, Ar is
and m is 1 or 2.
In certain embodiments, Ar is
In certain embodiments, X1 is H, halogen, —CN, or —OMe.
In certain embodiments, X1 is —OMe.
In certain embodiments, X2 is selected from the group consisting of H, halogen, —OH, —CN, C1-6 alkoxy, and CF3.
In certain embodiments, X2 is CF3.
In one aspect, the present disclosure relates to a pharmaceutical composition, comprising a compound described above; and a pharmaceutically acceptable carrier.
Definitions
The term “saturated” as used herein means the compound or group so modified has no carbon-carbon double and no carbon-carbon triple bonds, except as noted below. The term does not preclude carbon-heteroatom multiple bonds, for example a carbon oxygen double bond or a carbon nitrogen double bond. Moreover, it does not preclude a carbon-carbon double bond that may occur as part of keto-enol tautomerism or imine/enamine tautonerisn.
The term “alkyl” refers to a monovalent saturated aliphatic group with a carbon atom as the point of attachment, a linear or branched, cyclic or acyclic structure, and no atoms other than carbon and hydrogen. Thus, as used herein cycloalkyl is a subset of alkyl. The groups —CH3 (Me), —CH2CH3 (Et), —CH2CH2CH3 (n-Pr), —CH(CH3)2 (i-Pr), —CH(CH2)2 (cyclopropyl), —CH2CH2CH2CH3 (n-Bu), —CH(CH3)CH2CH3 (sec-butyl), —CH2CH(CH3)2 (iso-butyl), —C(CH3)3 (tert-butyl), —CH2C(CH3)3 (neopentyl), cyclobutyl, cyclopentyl, cyclohexyl, and cyclohexylmethyl are non-limiting examples of alkyl groups. The term “alkylene” refers to a divalent saturated aliphatic group, with one or two saturated carbon atom(s) as the point(s) of attachment, a linear or branched, cyclo, cyclic or acyclic structure, no carbon-carbon double or triple bonds, and no atoms other than carbon and hydrogen. The groups, —CH2— (methylene), —CH2CH2—, —CH2C(CH3)2CH2—, —CH2CH2CH2—, and
are non-limiting examples of alkylene groups.
The term “alkenyl” refers to a monovalent unsaturated aliphatic group with a carbon atom as the point of attachment, a linear or branched, cyclic or acyclic structure, at least one nonaromatic carbon-carbon double bond, no carbon-carbon triple bonds, and no atoms other than carbon and hydrogen. Non-limiting examples of alkenyl groups include: —CH═CH2 (vinyl), —CH═CHCH3, —CH═CHCH2CH3, —CH2CH═CH2 (allyl), —CH2CH═CHCH3, and —CH═CH—C6H5.
The term “alkynyl” refers to an monovalent unsaturated aliphatic group with a carbon atom as the point of attachment, a linear or branched, cyclo, cyclic or acyclic structure, at least one carbon-carbon triple bond, and no atoms other than carbon and hydrogen. As used herein, the term alkynyl does not preclude the presence of one or more non-aromatic carbon-carbon double bonds. The groups —C≡CH, —C≡CCH3, and —CH2C≡CCH3, are non-limiting examples of alkynyl groups.
The term “aryl” refers to a monovalent group with an aromatic carbon atom as the point of attachment, said carbon atom forming part of one or more six-membered aromatic ring structure(s) wherein the ring atoms are all carbon, and wherein the monovalent group consists of no atoms other than carbon and hydrogen. Non-limiting examples of aryl groups include phenyl (Ph), methylphenyl, (dimethyl)phenyl, −C6H4—CH2CH3 (ethylphenyl), —C6H4—CH2CH2CH3 (propylphenyl), —C6H4—CH(CH3)2, —C6H4—CH(CH2)2, —C6H3(CH3)CH2CH3 (methylethylphenyl), —C6H4—CH═CH2 (vinylphenyl), —C6H4—CH═CHCH3, —C6H4C≡CH, —C6H4C≡CCH3, naphthyl, and the monovalent group derived from biphenyl.
The term “heteroaryl” refers to a monovalent aromatic group with an aromatic carbon atom or nitrogen atom as the point of attachment, said carbon atom or nitrogen atom forming part of one or more aromatic ring structures wherein at least one of the ring atoms is nitrogen, oxygen or sulfur, and wherein the heteroaryl group consists of no atoms other than carbon, hydrogen, aromatic nitrogen, aromatic oxygen and aromatic sulfur. As used herein, the term does not preclude the presence of one or more alkyl, aryl, and/or aralkyl groups (carbon number limitation permitting) attached to the aromatic ring or aromatic ring system. If more than one ring is present, the rings may be fused or unfused. Non-limiting examples of heteroaryl groups include furanyl, imidazolyl, indolyl, indazolyl, isoxazolyl, methylpyridinyl, oxazolyl, phenylpyridinyl, pyridinyl, pyrrolyl, pyrimidinyl, pyrazinyl, quinolyl, quinazolyl, quinoxalinyl, triazinyl, tetrazolyl, thiazolyl, thienyl, and triazolyl.
The term “heterocyclyl” refers to a monovalent non-aromatic group with a carbon atom or nitrogen atom as the point of attachment, said carbon atom or nitrogen atom forming part of one or more non-aromatic ring structures wherein at least one of the ring atoms is nitrogen, oxygen or sulfur, and wherein the heterocyclyl group consists of no atoms other than carbon, hydrogen, nitrogen, oxygen and sulfur. As used herein, the term does not preclude the presence of one or more alkyl groups (carbon number limitation permitting) attached to the ring or ring system. If more than one ring is present, the rings may be fused or unfused. Non-limiting examples of heterocyclyl groups include aziridinyl, pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl, tetrahydrofuranyl, tetrahydrothiofuranyl, tetrahydropyranyl, and pyranyl.
The term “acyl” refers to the group —C(O)R, in which R is a hydrogen, alkyl, aryl, aralkyl or heteroaryl, as those terms are defined above. The groups —CHO, —C(O)CH3 (acetyl, Ac), —C(O)CH2CH3, C(O)CH2CH3, —C(O)CH(C3)2, —C(O)CH(CH2)2, —C(O)C6H5, —C(O)C6H4—CH3, —C(O)CH2C6H5, —C(O)(imidazolyl) are non-limiting examples of acyl groups. A “thioacyl” is defined in an analogous manner, except that the oxygen atom of the group —C(O)R has been replaced with a sulfur atom.
The term “alkoxy” refers to the group —OR, in which R is an alkyl, as that term is defined above. Non-limiting examples of alkoxy groups include: —OCH3, —OCH2CH3, —OCH2CH2CH3, —OCH(CH3)2, —OCH(CH2)2, —O-cyclopentyl, and —O-cyclohexyl.
The definition of “pharmaceutically acceptable” is meant to encompass any carrier, salt form, or other agent, which does not interfere with effectiveness of the biological activity of the active ingredient and that is not toxic to the host to which it is administered. As used herein, “pharmaceutically acceptable carrier” means buffers, carriers, diluents, and excipients suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio. The carrier(s) should be “acceptable” in the sense of being compatible with the other ingredients of the formulations and not deleterious to the recipient. Pharmaceutically acceptable carriers include buffers, solvents, dispersion media, coatings, isotonic and absorption delaying agents, and the like, that are compatible with pharmaceutical administration. “Pharmaceutically acceptable salts” means salts of compounds of the present disclosure which are pharmaceutically acceptable, and which possess the desired pharmacological activity. Such salts include acid addition salts formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like; or with organic acids such as 1,2-ethanedisulfonic acid, 2-hydroxyethanesulfonic acid, 2-naphthalenesulfonic acid, 3-phenylpropionic acid, 4,4′-methylenebis (3-hydroxy-2-ene-1-carboxylic acid), 4-methylbicyclo [2.2.2]oct-2-ene-1-carboxylic acid, acetic acid, aliphatic mono- and dicarboxylic acids, aliphatic sulfuric acids, aromatic sulfuric acids, benzenesulfonic acid, benzoic acid, camphorsulfonic acid, carbonic acid, cinnamic acid, citric acid, cyclopentanepropionic acid, ethanesulfonic acid, fumaric acid, glucoheptonic acid, gluconic acid, glutamic acid, glycolic acid, heptanoic acid, hexanoic acid, hydroxynaphthoic acid, lactic acid, laurylsulfuric acid, maleic acid, malic acid, malonic acid, mandelic acid, methanesulfonic acid, muconic acid, o-(4-hydroxybenzoyl)benzoic acid, oxalic acid, p-chlorobenzenesulfonic acid, phenyl-substituted alkanoic acids, propionic acid, p-toluenesulfonic acid, pyruvic acid, salicylic acid, stearic acid, succinic acid, tartaric acid, tertiarybutylacetic acid, trimethylacetic acid, and the like. Pharmaceutically acceptable salts also include base addition salts which may be formed when acidic protons present are capable of reacting with inorganic or organic bases. Acceptable inorganic bases include sodium hydroxide, sodium carbonate, potassium hydroxide, aluminum hydroxide and calcium hydroxide. Acceptable organic bases include ethanolamine, diethanolamine, triethanolamine, tromethamine, N-methylglucamine and the like. It should be recognized that the particular anion or cation forming a part of any salt of this invention is not critical, so long as the salt, as a whole, is pharmacologically acceptable. Additional examples of pharmaceutically acceptable salts and their methods of preparation and use are presented in Handbook of Pharmaceutical Salts Properties, and Use (P. H. Stahl & C. G. Wermuth eds., Verlag Helvetica Chimica Acta, 2002), which is incorporated herein by reference.
The compounds of the disclosure may contain one or more chiral centers and, therefore, exist as stereoisomers. The term “stereoisomers” when used herein consist of all enantiomers or diastereomers. These compounds may be designated by the symbols “(+),” “(−),” “R” or “S,” depending on the configuration of substituents around the stereogenic carbon atom, but the skilled artisan will recognize that a structure may denote a chiral center implicitly. The present invention encompasses various stereoisomers of these compounds and mixtures thereof. Mixtures of enantiomers or diastereomers may be designated “(±)” in nomenclature, but the skilled artisan will recognize that a structure may denote a chiral center implicitly.
The compounds of the disclosure may contain one or more double bonds and, therefore, exist as geometric isomers resulting from the arrangement of substituents around a carbon-carbon double bond. The symbol denotes a bond that may be a single, double or triple bond as described herein. Substituents around a carbon-carbon double bond are designated as being in the “Z” or “E” configuration wherein the terms “Z” and “E” are used in accordance with IUPAC standards. Unless otherwise specified, structures depicting double bonds encompass both the “E” and “Z” isomers. Substituents around a carbon-carbon double bond alternatively can be referred to as “cis” or “trans,” where “cis” represents substituents on the same side of the double bond and “trans” represents substituents on opposite sides of the double bond.
Compounds of the disclosure may contain a carbocyclic or heterocyclic ring and therefore, exist as geometric isomers resulting from the arrangement of substituents around the ring. The arrangement of substituents around a carbocyclic or heterocyclic ring are designated as being in the “Z” or “E” configuration wherein the terms “Z” and “E” are used in accordance with IUPAC standards. Unless otherwise specified, structures depicting carbocyclic or heterocyclic rings encompass both “Z” and “E” isomers. Substituents around a carbocyclic or heterocyclic ring may also be referred to as “cis” or “trans”, where the term “cis” represents substituents on the same side of the plane of the ring and the term “trans” represents substituents on opposite sides of the plane of the ring. Mixtures of compounds wherein the substituents are disposed on both the same and opposite sides of plane of the ring are designated “cis/trans.”
Individual enantiomers and diasteriomers of compounds of the present invention can be prepared synthetically from commercially available starting materials that contain asymmetric or stereogenic centers, or by preparation of racemic mixtures followed by resolution methods well known to those of ordinary skill in the art. These methods of resolution are exemplified by (1) attachment of a mixture of enantiomers to a chiral auxiliary, separation of the resulting mixture of diastereomers by recrystallization or chromatography and liberation of the optically pure product from the auxiliary, (2) salt formation employing an optically active resolving agent, (3) direct separation of the mixture of optical enantiomers on chiral liquid chromatographic columns or (4) kinetic resolution using stereoselective chemical or enzymatic reagents. Racemic mixtures can also be resolved into their component enantiomers by well-known methods, such as chiral-phase liquid chromatography or crystallizing the compound in a chiral solvent. Stereoselective syntheses, a chemical or enzymatic reaction in which a single reactant forms an unequal mixture of stereoisomers during the creation of a new stereocenter or during the transformation of a pre-existing one, are well known in the art. Stereoselective syntheses encompass both enantio- and diastereoselective transformations, and may involve the use of chiral auxiliaries. For examples, see Carreira and Kvaerno, Classics in Stereoselective Synthesis, Wiley-VCH: Weinheim, 2009.
The compounds disclosed herein can exist in solvated as well as unsolvated forms with pharmaceutically acceptable solvents such as water, ethanol, and the like, and it is intended that the invention embrace both solvated and unsolvated forms. In one embodiment, the compound is amorphous. In one embodiment, the compound is a single polymorph. In another embodiment, the compound is a mixture of polymorphs. In another embodiment, the compound is in a crystalline form.
The invention also embraces isotopically labeled compounds of the invention which are identical to those recited herein, except that one or more atoms are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes that can be incorporated into compounds of the invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, sulfur, fluorine and chlorine, such as 2H, 3H, 13C, 14C, 15N, 18O, 17O, 31P, 32P, 35S, 18F, and 36Cl, respectively. For example, a compound of the invention may have one or more H atom replaced with deuterium.
Certain isotopically-labeled disclosed compounds (e.g., those labeled with 3H and 14C) are useful in compound and/or substrate tissue distribution assays. Tritiated (i.e., 3H) and carbon-14 (i.e., 14C) isotopes are particularly preferred for their ease of preparation and detectability. Further, substitution with heavier isotopes such as deuterium (i.e., 2H) may afford certain therapeutic advantages resulting from greater metabolic stability (e.g., increased in vivo half-life or reduced dosage requirements) and hence may be preferred in some circumstances. Isotopically labeled compounds of the invention can generally be prepared by following procedures analogous to those disclosed in the examples herein by substituting an isotopically labeled reagent for a non-isotopically labeled reagent.
A “patient” or “subject” as described herein, refers to any animal at risk for, suffering from or diagnosed for an inflammatory condition, including, but not limited to, mammals, primates, and humans. In certain embodiments, the patient may be a non-human mammal such as, for example, a cat, a dog, or a horse. A patient may be an individual diagnosed with a high risk of developing an inflammatory condition, someone who has been diagnosed with an inflammatory condition, someone who previously suffered from an inflammatory condition, or an individual evaluated for symptoms or indications of an inflammatory condition.
“A patient in need”, as used herein, refers to a patient suffering from any of the symptoms or manifestations of an inflammatory condition, e.g., inflammatory bowel disease, a patient who may suffer from any of the symptoms or manifestations of an inflammatory condition, or any patient who might benefit from a method of the invention for treating an inflammatory condition. A patient in need may include a patient who is diagnosed with a risk of developing an inflammatory condition such as inflammatory bowel disease, a patient who has suffered from an inflammatory condition, or a patient who has previously been treated for such a condition.
The terms “treat”, “treatment”, “treating” and the like are used herein to generally mean obtaining a desired pharmacological and/or physiological effect. The effect may be prophylactic in terms of completely or partially preventing a disease or symptom thereof and/or may be therapeutic in terms of partially or completely curing a disease and/or adverse effect attributed to the disease. The term “treatment” as used herein covers any treatment of a disease in a mammal, particularly a human, and includes: (a) preventing the disease from occurring in a subject which may be predisposed to the disease but has not yet been diagnosed as having it; (b) inhibiting the disease, i.e. preventing the disease from increasing in severity or scope; (c) relieving the disease, i.e. causing partial or complete amelioration of the disease; or (d) preventing relapse of the disease, i.e. preventing the disease from returning to an active state following previous successful treatment of symptoms of the disease or treatment of the disease.
“Effective amount,” as used herein, refers to the amount of an agent that is sufficient to at least partially treat a condition when administered to a patient. The therapeutically effective amount will vary depending on the severity of the condition, the route of administration of the component, and the age, weight, etc. of the patient being treated. Accordingly, an effective amount of a compound of the present disclosure is the amount of such a compound necessary to treat one or more conditions or diseases contemplated herein in a patient such that administration of the agent prevents the condition(s) from occurring in a subject, prevents progression of the condition (e.g., prevents the onset or increased severity of symptoms of the condition), or relieves or completely ameliorates all associated symptoms of the condition, i.e. causes regression of the condition. An effective amount may also be the amount of such a compound necessary to bring about a desired biological effect, e.g. decreasing INF-γ levels.
Efficacy of treatment may be evaluated by means of evaluation of gross symptoms associated with the inflammatory condition, analysis of tissue histology, biochemical assay, imaging methods such as, for example, magnetic resonance imaging, or other known methods. For instance, efficacy of treatment may be evaluated by analyzing gross symptoms of the condition such as changes in tissue inflammation, abdominal pain, vomiting, diarrhea, rectal bleeding, cramps, muscle spasms, weight loss, malnutrition, fever, anemia or other aspects of gross pathology associated with an inflammatory condition following administration of a compound described herein.
Efficacy of treatment may also be evaluated at the tissue or cellular level, for example, by means of obtaining a tissue biopsy (e.g., a gastrointestinal tissue biopsy) and evaluating gross tissue or cell morphology or staining properties. Biochemical assays that examine protein or RNA expression may also be used to evaluate efficacy of treatment. For instance, one may evaluate IL-22, IFN-γ, or levels of another protein or gene product indicative of one or more inflammatory condition(s), inflammatory cytokine production, or an IL-22 mediated inflammatory response in dissociated cells or non-dissociated tissue via immunocytochemical, immunohistochemical, Western blotting, or Northern blotting methods, or methods useful for evaluating RNA levels such as quantitative or semi-quantitative polymerase chain reaction. One may also evaluate the presence or level of expression of useful biomarkers found in fecal matter, plasma, or serum to evaluate disease state and efficacy of treatment.
Methods
The present disclosure provides a method of treating an inflammatory condition in a patient in need thereof, comprising administering an effective amount of a compound disclosed herein to the patient. Exemplary inflammatory conditions contemplated herein include Crohn's disease, gastroduodenal Crohn's disease, Crohn's (granulomatous) colitis, ulcerative colitis, collagenous colitis, lymphocytic colitis, ischaemic colitis, diversion colitis, Behçet's disease, microscopic colitis, ulcerative proctitis, proctosigmoiditis, jejunoileitis, left-sided colitis, pancolitis, ileocolitis, ileitis, indeterminate colitis and eosinophilic enteritis. Other contemplated inflammatory conditions include cartilage inflammation, bone degradation, rheumatoid arthritis, arthritis, psoriatic arthritis, hypersensitivity pneumonitis, liver diseases such as fatty liver, hepatitis, hepatic steatosis, and nonalcoholic steatohepatitis (NASH), fibrosis (e.g., intestinal fibrosis, lung fibrosis, or liver fibrosis), autoimmune polyendocrine syndrome, Addison's disease, Goodpasture's syndrome, Graves' disease, Guillain-Barré syndrome, Hashimoto's encephalopathy, psoriasis, Hashimoto's thyroiditis, juvenile arthritis (e.g., juvenile idiopathic arthritis), lupus erythematous, multiple sclerosis, Alzheimer's disease, dermatitis, atopic dermatitis, acne, Type I diabetes mellitus, autoimmune hepatitis, Ménière's disease, Raynaud's phenomenon, Sjögren's syndrome, ankylosing spondylitis, chronic fatigue syndrome, rheumatic polymyalgia, osteoarthritis, prostatitis, and Reiter syndrome. In certain embodiments the patient is a mammal. In certain other embodiments the patient is a human.
In certain embodiments, a method of treating an inflammatory disease or condition is provided, wherein the disease or condition is selected from the group consisting of inflammatory bowel disease, cartilage inflammation, bone degradation, ulcerative colitis, psoriasis, arthritis, psoriatic arthritis, rheumatoid arthritis, juvenile arthritis, juvenile rheumatoid arthritis, autoimmune hepatitis, Crohn's disease, lupus erythematosus, multiple sclerosis, Alzheimer's disease, dermatitis, atopic dermatitis, acne, Type I diabetes mellitus, Raynaud's phenomenon, Graves' disease, and Addison's disease, and wherein the method comprises administering to a patient in need thereof an effective amount of a disclosed compound. In certain embodiments the patient is a mammal. In certain other embodiments the patient is a human.
A method of treating Crohn's disease is provided, and/or ulcerative colitis, and/or inflammatory bowel disease, comprising administering to a patient in need thereof an effective amount of a disclosed compound. In certain embodiments the patient is a mammal. In certain other embodiments the patient is a human.
In certain embodiments, the inflammatory disease or condition is selected from the group consisting of Crohn's disease, ulcerative colitis, collagenous colitis, lymphocytic colitis, diversion colitis, Behçet's disease, idiopathic inflammatory bowel disease, irritable bowel syndrome, regional enteritis, spastic colon, microscopic colitis, Crohn's colitis, perianal disease, and indeterminate colitis.
In certain embodiments, the inflammatory disease or condition is Crohn's disease. In certain embodiments, the Crohn's disease is selected from the group consisting of ileocolitis, ileitis, gastroduodenal Crohn's disease, jejunoileitis, and granulomatous ileocolitis. In certain embodiments, the Crohn's disease includes intestinal fibrosis. In certain embodiments, the Crohn's disease is fibrostenotic Crohn's disease.
In another aspect, the present disclosure provides a method of preventing, treating, or reducing fibrostenosis or intestinal fibrosis in a subject, comprising administering to a subject in need thereof a therapeutically effective amount of a compound disclosed herein. In certain embodiments, the fibrostenosis or intestinal fibrosis is associated with Crohn's disease.
It may be appreciated that inflammatory bowel disease may be associated with a number of symptoms. Accordingly, the present disclosure provides a method of relieving one or more symptoms of inflammatory bowel disease selected from the group consisting of abdominal pain, vomiting, diarrhea, rectal bleeding, severe cramps, muscle spasms, weight loss, malnutrition, fever, anemia, skin lesions, joint pain, eye inflammation, liver disorders, arthritis, pyoderma gangrenosum, primary sclerosing cholangitis, non-thyroidal illness syndrome, and growth defects in children, comprising administering to a patient in need thereof an effective amount of a disclosed compound.
In one aspect, the present disclosure relates to a method of decreasing IFN-γ levels and/or inhibiting IFN-γ, comprising administering to a subject in need thereof a therapeutically effective amount of a disclosed compound. “Inhibiting IFN-γ,” as used herein, may refer to a complete or partial reduction in IFN-γ expression or activity. Thus, inhibiting IFN-γ may refer to alterations in IFN-γ gene or chromatin state or altered interaction with regulators of gene transcription or gene accessibility that results in a complete or partial reduction in expression of IFN-γ gene products, e.g., IFN-γ RNA, IFN-γ protein, or peptide sequences of IFN-γ. Inhibiting IFN-γ may also refer to inhibition of processes crucial to IFN-γ gene product expression, including, but not limited to IFN-γ transcription, IFN-γ RNA processing, IFN-γ protein translation, or IFN-γ post-translational modification. Additionally, inhibiting IFN-γ may refer to inhibiting activity of IFN-γ gene products, including peptides of IFN-γ, nucleotide products of IFN-γ (e.g., IFN-γ mRNA), and IFN-γ protein. Inhibiting activity of IFN-γ gene products may include a reduction in IFN-γ signaling or direct or indirect interaction of IFN-γ with other cellular components (e.g., proteins, peptides, DNA, RNA, lipids, or signaling molecules) including nuclear, organelle, cytosolic, membrane, and extracellular components. For example, inhibiting IFN-γ activity may include inhibiting IFN-γ binding or activation of CSF1R or inhibiting CSF1R downstream signaling effects (e.g., MAP Kinase phosphorylation or macrophage proliferation).
In one aspect, the present disclosure relates to a method of increasing IL-22 levels, comprising administering to a subject in need thereof a therapeutically effective amount of a disclosed compound. Such increase may be for improving or strengthening immunity or to provide an immunoprotective function.
The invention also provides methods of increasing IL-22 production in cells of a patient suffering from an inflammatory condition, comprising administering to a patient in need thereof an effective amount of a disclosed compound. IL-22 production may be increased in any cell in which IL-22 expression or activity occurs, including cells of the gastrointestinal tract, immune system, and blood. Cells of the gastrointestinal tract (including cells of the stomach, duodenum, jejunum, ileum, colon, rectum and anal canal), include columnar epithelial cells, mucosal epithelial cells, zymogenic cells, neck mucus cells, parietal cells, gastrin cells, goblet cells, paneth cells, oligomucus cells, and villus absorptive cells. Cells of the immune system include leukocytes, phagocytes (e.g., macrophages, neutrophils, and dendritic cells), monocytes, mast cells, eosinophils, basophils, natural killer cells, innate cells, lymphocytes, B cells, and T cells. Blood cells include red blood cells (erythrocytes) and white blood cells (leukocytes, monocytes, and platelets).
In one aspect, the present disclosure relates to a method of increasing IL-22 levels and decreasing IFN-γ levels, comprising administering to a subject in need thereof a therapeutically effective amount of a compound described above.
In one aspect, the present disclosure relates to a method of inhibiting lipid peroxidation, comprising administering to a subject in need thereof a therapeutically effective amount of a compound described above.
In one aspect, the present disclosure relates to a method of modulating an aryl hydrocarbon receptor (AhR), comprising administering a selective AhR modulator; wherein said selective AhR modulator is a compound described above.
In another aspect, the present invention provides a compound as described herein for use as a medicament. In another aspect, the present invention provides a compound as described herein for use in a method of treating an inflammatory disease or condition. The inflammatory disease or condition may be as defined above. In yet another aspect, the present invention provides a compound as described herein for use in a method of treating Crohn's disease and/or ulcerative colitis, and/or inflammatory bowel disease. The Crohn's disease may be as defined above. In another aspect, the present disclosure provides a compound as described herein for use in a method of preventing, treating, or reducing fibrostenosis or intestinal fibrosis. In certain embodiments, the fibrostenosis or intestinal fibrosis is associated with Crohn's disease. In another embodiment, the present invention provides a compound as described herein for use in a method of relieving one or more symptoms of inflammatory bowel disease. The symptoms may be as defined above. In one aspect, the present invention provides a compound as described herein relates to a method of decreasing IFN-γ levels and/or inhibiting IFN-γ in a subject in need thereof. Inhibiting IFN-γ may be as defined above. In a further aspect, the present provides a compound as described herein for use in a method of increasing IL-22 levels in a subject. Such increase may be for improving or strengthening immunity or to provide an immunoprotective function. The invention also provides a compound as described above for use in a method of increasing IL-22 production in cells of a patient suffering from an inflammatory condition. The inflammatory condition may be as defined above. In another aspect, the present invention provides a compound as described herein for use in a method of increasing IL-22 levels and decreasing IFN-γ levels in a subject. The present invention also provides a compound as described herein for use in a method of inhibiting lipid peroxidation in a subject. The present invention also provides a compound for use in a method of modulating an aryl hydrocarbon receptor (AhR) in a subject.
Pharmaceutical Compositions and Routes of Administration
The present invention also provides pharmaceutical compositions comprising a compound described herein. In another aspect, the invention provides a pharmaceutical composition for use in treating an inflammatory condition. The pharmaceutical composition may comprise a compound described herein and a pharmaceutically acceptable carrier. As used herein the term “pharmaceutical composition” means, for example, a mixture containing a specified amount of a therapeutic compound, e.g., a therapeutically effective amount, of a therapeutic compound in a pharmaceutically acceptable carrier to be administered to a mammal, e.g., a human, in order to treat an inflammatory condition.
Compounds of the present disclosure can be specially formulated for administration of the compound to a subject in solid, liquid or gel form, including those adapted for the following: (1) oral administration, for example, drenches (aqueous or non-aqueous solutions or suspensions), lozenges, dragees, capsules, pills, tablets (e.g., those targeted for buccal, sublingual, and systemic absorption), boluses, powders, granules, pastes for application to the tongue; (2) parenteral administration, for example, by subcutaneous, intramuscular, intravenous or epidural injection as, for example, a sterile solution or suspension, or sustained-release formulation; (3) topical application, for example, as a cream, ointment, or a controlled-release patch or spray applied to the skin; (4) intravaginally or intrarectally, for example, as a pessary, cream or foam; (5) sublingually; (6) ocularly; (7) transdermally; (8) transmucosally; or (9) nasally. Examples of dosage forms include, but are not limited to: tablets; caplets; capsules, such as hard gelatin capsules and soft elastic gelatin capsules; cachets; troches; lozenges; dispersions; suppositories; ointments; cataplasms (poultices); pastes; powders; dressings; creams; plasters; solutions; patches; aerosols (e.g., nasal sprays or inhalers); gels; liquids such as suspensions (e.g., aqueous or non-aqueous liquid suspensions, oil-in-water emulsions, or water-in-oil liquid emulsions), solutions, and elixirs; and sterile solids (e.g., crystalline or amorphous solids) that can be reconstituted to provide liquid dosage forms.
Pharmaceutical compositions containing a compound described herein can be presented in a dosage unit form and can be prepared by any suitable method. A pharmaceutical composition should be formulated to be compatible with its intended route of administration. Useful formulations can be prepared by methods well known in the pharmaceutical art. For example, see Remington's Pharmaceutical Sciences, 18th ed. (Mack Publishing Company, 1990).
Pharmaceutical formulations preferably are sterile. Sterilization can be accomplished, for example, by filtration through sterile filtration membranes. Where the composition is lyophilized, filter sterilization can be conducted prior to or following lyophilization and reconstitution.
The pharmaceutical compositions of the invention can be formulated for parenteral administration, e.g., formulated for injection via the intravenous, intramuscular, subcutaneous, intralesional, or intraperitoneal routes. The preparation of an aqueous composition, such as an aqueous pharmaceutical composition containing a compound described herein, will be known to those of skill in the art in light of the present disclosure. Typically, such compositions can be prepared as injectables, either as liquid solutions or suspensions; solid forms suitable for using to prepare solutions or suspensions upon the addition of a liquid prior to injection can also be prepared; and the preparations can also be emulsified.
The pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions; formulations including sesame oil, peanut oil or aqueous propylene glycol; and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases the form must be sterile and must be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms, such as bacteria and fungi.
Solutions of active compounds as free base or pharmacologically acceptable salts can be prepared in water suitably mixed with a surfactant, such as hydroxypropylcellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycols, and mixtures thereof and in oils. In addition, sterile, fixed oils may be employed as a solvent or suspending medium. For this purpose any bland fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid can be used in the preparation of injectables. The sterile injectable preparation may also be a sterile injectable solution, suspension, or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, U.S.P., and isotonic sodium chloride solution. In one embodiment, the compound may be suspended in a carrier fluid comprising 1% (w/v) sodium carboxymethylcellulose and 0.1% (v/v) TWEEN™ 80. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms.
Injectable preparations, for example, sterile injectable aqueous or oleaginous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. Generally, dispersions are prepared by incorporating the various sterilized active ingredients into a sterile vehicle which contains the basic dispersion medium and the required other ingredients from those enumerated above. Sterile injectable solutions of the invention may be prepared by incorporating a compound described herein in the required amount of the appropriate solvent with various of the other ingredients enumerated above, as required, followed by filtered sterilization. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum-drying and freeze-drying techniques which yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof. The injectable formulations can be sterilized, for example, by filtration through a bacteria-retaining filter.
The preparation of more, or highly concentrated solutions for intramuscular injection is also contemplated. In this regard, the use of DMSO as solvent is preferred as this will result in extremely rapid penetration, delivering high concentrations of the compound to a small area.
Suitable preservatives for use in such a solution include benzalkonium chloride, benzethonium chloride, chlorobutanol, thimerosal and the like. Suitable buffers include boric acid, sodium and potassium bicarbonate, sodium and potassium borates, sodium and potassium 10 carbonate, sodium acetate, sodium biphosphate and the like, in amounts sufficient to maintain the pH at between about pH 6 and pH 8, and preferably, between about pH 7 and pH 7.5. Suitable tonicity agents are dextran 40, dextran 70, dextrose, glycerin, potassium chloride, propylene glycol, sodium chloride, and the like, such that the sodium chloride equivalent of the solution is in the range 0.9 plus or minus 0.2%. Suitable antioxidants and stabilizers include sodium bisulfite, sodium metabisulfite, sodium thiosulfite, thiourea and the like. Suitable wetting and clarifying agents include polysorbate 80, polysorbate 20, poloxamer 282 and tyloxapol. Suitable viscosity-increasing agents include dextran 40, dextran 70, gelatin, glycerin, hydroxyethylcellulose, hydroxymethylpropylcellulose, lanolin, methylcellulose, petrolatum, polyethylene glycol, polyvinyl alcohol, polyvinylpyrrolidone, carboxymethylcellulose and the like.
In some embodiments, contemplated herein are compositions suitable for oral delivery of a compound described herein, e.g., tablets, that include an enteric coating, e.g., a gastro-resistant coating, such that the compositions may deliver the compound to, e.g., the gastrointestinal tract of a patient.
For example, a tablet for oral administration is provided that comprises granules (e.g., is at least partially formed from granules) that include a compound described herein, and one or more pharmaceutically acceptable excipients. Such a tablet may be coated with an enteric coating. Contemplated tablets may include pharmaceutically acceptable excipients such as fillers, binders, disintegrants, and/or lubricants, as well as coloring agents, release agents, coating agents, sweetening, flavoring agents such as wintergreen, orange, xylitol, sorbitol, fructose, and maltodextrin, and perfuming agents, preservatives and/or antioxidants.
In some embodiments, contemplated pharmaceutical formulations include an intra-granular phase that includes a compound described herein, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable filler. For example, the compound and a filler may be blended together, optionally, with other excipients, and formed into granules. In some embodiments, the intragranular phase may be formed using wet granulation, e.g. a liquid (e.g., water) is added to the blended compound and filler, and then the combination is dried, milled and/or sieved to produce granules. One of skill in the art would understand that other processes may be used to achieve an intragranular phase.
In some embodiments, contemplated formulations include an extra-granular phase, which may include one or more pharmaceutically acceptable excipients, and which may be blended with the intragranular phase to form a disclosed formulation.
A disclosed formulation may include an intragranular phase that includes a filler. Exemplary fillers include, but are not limited to, cellulose, gelatin, calcium phosphate, lactose, sucrose, glucose, mannitol, sorbitol, microcrystalline cellulose, pectin, polyacrylates, dextrose, cellulose acetate, hydroxypropylmethyl cellulose, partially pregelatinized starch, calcium carbonate, and others including combinations thereof.
In some embodiments, a disclosed formulation may include a intragranular phase and/or a extragranular phase that includes a binder, which may generally function to hold the ingredients of the pharmaceutical formulation together. Exemplary binders of the invention may include, but are not limited to, the following: starches, sugars, cellulose or modified cellulose such as hydroxypropyl cellulose, lactose, pregelatinized maize starch, polyvinyl pyrrolidone, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, low substituted hydroxypropyl cellulose, sodium carboxymethyl cellulose, methyl cellulose, ethyl cellulose, sugar alcohols and others including combinations thereof.
Contemplated formulations, e.g., that include an intragranular phase and/or an extragranular phase, may include a disintegrant such as but are not limited to, starch, cellulose, crosslinked polyvinyl pyrrolidone, sodium starch glycolate, sodium carboxymethyl cellulose, alginates, corn starch, crosmellose sodium, crosslinked carboxymethyl cellulose, low substituted hydroxypropyl cellulose, acacia, and others including combinations thereof. For example, an intragranular phase and/or an extragranular phase may include a disintegrant.
In some embodiments, a contemplated formulation includes an intra-granular phase comprising a compound described herein and excipients chosen from: mannitol, microcrystalline cellulose, hydroxypropylmethyl cellulose, and sodium starch glycolate or combinations thereof, and an extra-granular phase comprising one or more of: microcrystalline cellulose, sodium starch glycolate, and magnesium stearate or mixtures thereof.
In some embodiments, a contemplated formulation may include a lubricant, e.g. an extra-granular phase may contain a lubricant. Lubricants include but are not limited to talc, silica, fats, stearin, magnesium stearate, calcium phosphate, silicone dioxide, calcium silicate, calcium phosphate, colloidal silicon dioxide, metallic stearates, hydrogenated vegetable oil, corn starch, sodium benzoate, polyethylene glycols, sodium acetate, calcium stearate, sodium lauryl sulfate, sodium chloride, magnesium lauryl sulfate, talc, and stearic acid.
In some embodiments, the pharmaceutical formulation comprises an enteric coating. Generally, enteric coatings create a barrier for the oral medication that controls the location at which the drug is absorbed along the digestive tract. Enteric coatings may include a polymer that disintegrates a different rates according to pH. Enteric coatings may include for example, cellulose acetate phthalate, methyl acrylate-methacrylic acid copolymers, cellulose acetate succinate, hydroxylpropylmethyl cellulose phthalate, methyl methacrylate-methacrylic acid copolymers, ethylacrylate-methacrylic acid copolymers, methacrylic acid copolymer type C, polyvinyl acetate-phthalate, and cellulose acetate phthalate.
Exemplary enteric coatings include Opadry® AMB, Acryl-EZE®, Eudragit® grades. In some embodiments, an enteric coating may comprise about 5% to about 10%, about 5% to about 20%, 8 to about 15%, about 8% to about 20%, about 10% to about 20%, or about 12 to about 20%, or about 18% of a contemplated tablet by weight. For example, enteric coatings may include an ethylacrylate-methacrylic acid copolymer.
For example, in a contemplated embodiment, a tablet is provided that comprises or consists essentially of about 0.5% to about 70%, e.g. about 0.5% to about 10%, or about 1% to about 20%, by weight of a compound described herein or a pharmaceutically acceptable salt thereof. Such a tablet may include for example, about 0.5% to about 60% by weight of mannitol, e.g. about 30% to about 50% by weight mannitol, e.g. about 40% by weight mannitol; and/or about 20% to about 40% by weight of microcrystalline cellulose, or about 10% to about 30% by weight of microcrystalline cellulose. For example, a disclosed tablet may comprise an intragranular phase that includes about 30% to about 60%, e.g. about 45% to about 65% by weight, or alternatively, about 5 to about 10% by weight of a compound described herein, about 30% to about 50%, or alternatively, about 5% to about 15% by weight mannitol, about 5% to about 15% microcrystalline cellulose, about 0% to about 4%, or about 1% to about 7% hydroxypropylmethylcellulose, and about 0% to about 4%, e.g. about 2% to about 4% sodium starch glycolate by weight.
In another contemplated embodiment, a pharmaceutical tablet formulation for oral administration of a compound described herein comprises an intra-granular phase, wherein the intra-granular phase includes a compound described herein or a pharmaceutically acceptable salt thereof (such as a sodium salt), and a pharmaceutically acceptable filler, and which may also include an extra-granular phase, that may include a pharmaceutically acceptable excipient such as a disintegrant. The extra-granular phase may include components chosen from microcrystalline cellulose, magnesium stearate, and mixtures thereof. The pharmaceutical composition may also include an enteric coating of about 12% to 20% by weight of the tablet. For example, a pharmaceutically acceptable tablet for oral use may comprise about 0.5% to 10% by weight of a compound described herein or a pharmaceutically acceptable salt thereof, about 30% to 50% by weight mannitol, about 10% to 30% by weight microcrystalline cellulose, and an enteric coating comprising an ethylacrylate-methacrylic acid copolymer.
In another example, a pharmaceutically acceptable tablet for oral use may comprise an intra-granular phase, comprising about 5 to about 10% by weight of a compound described herein or a pharmaceutically acceptable salt thereof, about 40% by weight mannitol, about 8% by weight microcrystalline cellulose, about 5% by weight hydropropylmethyl cellulose, and about 2% by weight sodium starch glycolate; an extra-granular phase comprising about 17% by weight microcrystalline cellulose, about 2% by weight sodium starch glycolate, about 0.4% by weight magnesium stearate; and an enteric coating over the tablet comprising an ethylacrylate-methacrylic acid copolymer.
In some embodiments the pharmaceutical composition may contain an enteric coating comprising about 13% or about 15%, 16%, 17% or 18% by weight, e.g., AcyrlEZE® (see, e.g., PCT Publication No. WO2010/054826, which is hereby incorporated by reference in its entirety).
The rate at which point the coating dissolves and the active ingredient is released is its dissolution rate. In an embodiment, a contemplated tablet may have a dissolution profile, e.g. when tested in a USP/EP Type 2 apparatus (paddle) at 100 rpm and 37° C. in a phosphate buffer with a pH of 7.2, of about 50% to about 100% of a compound described herein releasing after about 120 minutes to about 240 minutes, for example after 180 minutes. In another embodiment, a contemplated tablet may have a dissolution profile, e.g. when tested in a USP/EP Type 2 apparatus (paddle) at 100 rpm and 37° C. in diluted HCl with a pH of 1.0, where substantially none of the compound is released after 120 minutes. A contemplated tablet, in another embodiment, may have a dissolution profile, e.g. when tested in USP/EP Type 2 apparatus (paddle) at 100 rpm and 37° C. in a phosphate buffer with a pH of 6.6, of about 10% to about 30%, or not more than about 50%, of the compound releasing after 30 minutes.
In some embodiments, methods provided herein may further include administering at least one other agent that is directed to treatment of conditions, diseases, and disorders disclosed herein. In one embodiment, contemplated other agents may be co-administered (e.g., sequentially or simultaneously).
Non-limiting examples of such combination therapy include combination of one or more compounds of the invention with an anti-inflammatory agent, an antibiotic agent, an immunosuppressant, an inmmunomodulator, or an analgesic agent.
Agents contemplated include immunosuppressive agents including glucocorticoids, cytostatics, antibodies, agents acting on immunophilins, interferons, opioids, TNF binding proteins, mycophenolate, and small biological agents. For example, contemplated immunosuppressive agents include, but are not limited to: tacrolimus, cyclosporine, pimecrolimus, sirolimus, everolimus, mycophenolic acid, fingolimod, dexamethasone, fludarabine, cyclophosphamide, methotrexate, azathioprine, leflunomide, teriflunomide, anakinra, anti-thymocyte globulin, anti-lymphocyte globulin, muromonab-CD3, afutuzumab, rituximab, teplizumab, efalizumab, daclizumab, basiliximab, adalimumab, infliximab, certolizumab pegol, natalizumab, and etanercept. Other contemplated agents include anti-diarrheals, laxatives, iron supplements, and calcium or vitamin D or B-12 supplements.
Exemplary formulations include dosage forms that comprise or consist essentially of about 35 mg to about 500 mg of a compound described herein. For example, formulations that include about 35 mg, 40 mg, 50 mg, 60 mg, 70 mg, 80 mg, 90 mg, 100 mg, 110 mg, 120 mg, 130 mg, 140 mg, 150 mg, 160 mg, 170 mg, 180 mg, 190 mg, 200 mg, or 250 mg of a compound described herein are contemplated. In one embodiment, a formulation may include about 40 mg, 80 mg, or 160 mg of a compound described herein. In some embodiments, a formulation may include at least 100 μg of a compound described herein. For example, formulations may include about 0.1 mg, 0.2 mg, 0.3 mg, 0.4 mg, 0.5 mg, 1 mg, 5 mg, 10 mg, 15 mg, 20 mg, or 25 mg of a compound described herein. The amount administered will depend on variables such as the type and extent of disease or indication to be treated, the overall health and size of the patient, the in vivo potency of the compound, the pharmaceutical formulation, and the route of administration. The initial dosage can be increased beyond the upper level in order to rapidly achieve the desired blood-level or tissue level. Alternatively, the initial dosage can be smaller than the optimum, and the dosage may be progressively increased during the course of treatment. Dosing frequency can vary, depending on factors such as route of administration, dosage amount and the disease being treated. Exemplary dosing frequencies are once per day, once per week and once every two weeks. In some embodiments, dosing is once per day for 7 days.
The invention is further illustrated by the following examples. The examples are provided for illustrative purposes only, and are not to be construed as limiting the scope or content of the invention in any way.
Sample 2 was prepared as shown below in Scheme 1. Compound numbers used in this Example pertain only to this Example; Sample 2 is referred to elsewhere as compound 2.
A mixture of 2-iodo-4-(trifluoromethyl)aniline (1) (5.00 g, 17.4 mmol), 4-(triethylsilyl)but-3-yn-1-ol (2) (3.85 g, 1.2 mmol), bis(diphenylphosphino)ferrocene]palladium(II) chloride (0.64 g, 0.87 mmol), lithium chloride (0.732 g, 17.4 mmol) and sodium carbonate (3.7 g, 34.8 mmol)) in 50 mL of N,N-dimethylformamide (DMF) was stirred at 100° C. for 15 h. The reaction mixture was diluted with water and extracted with ethyl acetate. The organic extract was dried over Na2SO4 and concentrated under reduced pressure. The crude material was purified by silica gel chromatography to afford 7.00 g of the title compound as yellow oil, which contained ˜20% of starting material (2). The product was used in the next step without further purification. 1H NMR (300 MHz, CDCl3) δ 8.2 (bs, 1H), 7.9 (s, 1H), 7.45 (s, 2H), 3.85 (q, J=8.0 Hz, 2H), 3.15 (t, J=6.4 Hz, 2H), 1.46 (t, J=6.0 Hz, 1H), 1.2 (m, 15H); MS (APCI+) m/z=344 (M+H).
A solution of 2-(2-(triethylsilyl)-5-(trifluoromethyl)-1H-indol-3-yl)ethanol (3) (2.00 g, 5.83 mmol) in 15 mL of tetrahydrofuran (THF) was added tetrabutylammonium fluoride (7.0 mL, 1 M in THF) and the reaction mixture was stirred at room temperature (rt) for 72 h. The reaction mixture was diluted with water and extracted with ethyl acetate. The organic extract was dried over Na2SO4 and concentrated under reduced pressure. The crude material was purified by silica gel chromatography to afford 1.02 g (75%) of the title compound as pale yellow oil. 1H NMR (300 MHz, CDCl3) δ 8.3 (bs, 1H), 7.96 (s, 1H), 7.45 (s, 2H), 7.2 (s, 1H), 3.90 (t, J=6.8 Hz, 2H), 3.05 (t, J=6.8 Hz, 2H); MS (APCI+) m/z=230 (M+H).
A solution of 2-(5-(trifluoromethyl)-1H-indol-3-yl)ethanol (4) (1.00 g, 4.36 mmol) in THF (10 ml) was added to a solution of triphenyl phosphine (2.30 g, 8.72 mmol) and perbromomethane (4.40 g, 13.1 mmol) in THF (10 mL) pre-stirred for 1 h. The resulting mixture was stirred at rt for 3 h. The reaction mixture was then filtered and concentrated under reduced pressure. The crude material was purified by silica gel chromatography to afford 0.66 g (51%) of the title compound as yellow oil. 1H NMR (300 MHz, CDCl3) δ 8.25 (bs, 1H), 7.9 (s, 1H), 7.4 (s, 2H), 7.2 (s, 1H), 3.7 (t, J=7.8 Hz, 2H), 3.27 (t, J=7.8 Hz, 2H).
A mixture of 3-(2-bromoethyl)-5-(trifluoromethyl)-1H-indole (5) (0.66 g, 2.26 mmol) and sodium azide (0.44 g; 6.8 mmol) in DMF (10 mL) was stirred at 70° C. for 4 h. The reaction mixture was diluted with water and extracted with ethyl acetate. The organic layer was successively washed with brine, sodium thiosulfate, dried and concentrated under reduced pressure. The crude material was purified by silica gel chromatography to afford 0.61 g (100%) of the title compound as a brown oil. 1H NMR (300 MHz, CDCl3) δ 8.15 (bs, 1H), 7.87 (s, 1H), 7.44 (d, J=1.5 Hz, 2H), 7.19 (s, 1H), 3.55-3.61 (t, J=7 Hz, 2H), 3.08 (t, J=7.2 Hz, 2H).
A mixture of 3-(2-azidoethyl)-5-(trifluoromethyl)-H-indole (6) (0.61 g, 2.45 mmol) and triphenyl phosphine (1.93 g, 7.41 mmol) in methanol (10 mL) was stirred at 70° C. for 2 h. The reaction mixture was concentrated under reduced pressure and the crude material was purified by silica gel chromatography to afford 0.44 g (75%) of the title compound as a brown oil. 1H NMR (300 MHz, CDCl3) δ 8.4 (bs, 1H), 7.9 (s, 1H), 7.4 (s, 2H), 7.25 (s, 1H), 7.13-7.14 (d, J=2.4 Hz, 1H), 3.04 (m, 4H); MS (APCI−) m/z=326 (M−H).
A mixture of 2-(5-(trifluoromethyl)-1H-indol-3-yl))ethanamine (7) (0.40 g, 1.76 mmol) and 3,4-dimethoxybenzaldehyde (8) (0.322 g, 1.93 mmol) in acetic acid (8 ml) was stirred at 80° C. for 24 h. The reaction mixture was concentrated under reduced pressure and the crude material was purified by silica gel chromatography to afford 0.16 g (33%) of the title compound as a white solid. 1H NMR (300 MHz, CDCl3) δ 7.9 (s, 1H), 7.71 (bs, 1H), 7.33 (m, 1H), 6.84 (d, J=3.8 Hz, 2H), 5.12 (s, 1H), 3.88 (s, 3H), 3.81 (s, 3H), 3.15 (m, 1H), 2.88 (m, 2H); MS (APCI+) m/z=377 (M+H).
Sample 4a was prepared as shown below in Scheme 2. Compound numbers used in this Example pertain only to this Example; Sample 4a is referred to elsewhere as compound 4a.
A solution of 2-iodo-1H-indole (1) (1.50 g, 6.17 mmol) in THF (20 mL) was added to a suspension of 60% NaH (0.37 g, 9.25 mmol) at 0° C. and the resulting solution was stirred for 10 min. Methyl iodide (1.75 g, 12.3 mmol) was added dropwise and the reaction mixture was allowed to warm slowly from 0° C. to rt over 1 h. The reaction was quenched with saturated NH4Cl solution (15 mL) and extracted with ethyl acetate. The organic layer was dried over Na2SO4 and concentrated under reduced pressure. The crude material was purified by silica gel chromatography to afford 1.27 g (80%) of the title compound as a pale yellow oil. 1H NMR (400 MHz, CDCl3) δ 7.71 (d, J=7.6 Hz, 1H), 7.29 (d, J=8 Hz, 1H), 7.14 (t, J=7.6 Hz, 1H), 7.06 (t, J=7.6 Hz, 1H), 6.78 (s, 1H), 3.74 (s, 3H).
To a stirred solution of acrolein (3) (1.38 g, 24.7 mmol) and N-methylaniline (0.16 g, 1.48 mmol) at 0° C. was added TFA (0.16 g, 1.48 mmol) dropwise and the reaction mixture was stirred at 0° C. for 10 min. 2-Iodo-1-methyl-1H-indole (2) (1.27 g, 4.94 mmol) in CH2Cl2 (4 mL) was added and the reaction mixture was slowly stirred from 0° C. to rt for 3 h. The reaction mixture was diluted with water and extracted with dichloromethane (DCM). The organic layer was dried over Na2SO4 and concentrated under reduced pressure. The crude material was purified by silica gel chromatography to afford 920 mg (59%) of the title compound as light yellow foam. 1H NMR (400 MHz, CDCl3) δ 9.85 (s, 2H), 7.51 (d, J=8.0 Hz, 1H), 7.30 (d, J=8.4 Hz, 1H), 7.16 (t, J=9.8 Hz, 1H), 7.08 (t, J=8 Hz, 1H), 3.75 (s, 3H), 3.75 (s, 3H), 3.09 (t, J=7.6 Hz, 2H), 2.76 (t, J=8.2 Hz, 2H); MS (ESI+) m/z=314 (M+H).
To a solution of 3-(2-iodo-1-methyl-1H-indol-3-yl)propanal (4) (920 mg, 2.93 mmol) and 3,4,5-trimethoxyaniline (5) (806 mg, 4.40 mmol) in methanol (15 mL) was added 1 drop of acetic acid and the reaction mixture was stirred for 10 min. NaCNBH3 (0.46 g, 7.32 mmol) was added and stirring continued for 16 h. The reaction mixture was diluted with water and extracted with DCM. The organic layer was dried over Na2SO4 and concentrated under reduced pressure. The crude material was purified by silica gel chromatography to afford 1.07 g (74%) of the title compound as an off-white foam. 1H NMR (400 MHz, CDCl3) δ 7.53 (d, J=7.8 Hz, 1H), 7.31 (d, J=8.1 Hz, 1H), 7.16 (t, J=9 Hz, 1H), 7.06 (t, J=7.8 Hz, 1H), 5.77 (s, 2H), 3.75 (s, 3H), 3.14 (t, J=7.05 Hz, 2H), 2.88 (t, J=7.2 Hz, 2H), 1.26 (m, 2H); MS (ESI+) m/z=481 (M+H).
To a solution of N-(3-(2-iodo-1-methyl-1H-indol-3-yl))propyl)-3,4,5-trimethoxyaniline (6) (1.07 g, 2.22 mmol) in toluene (5 mL) was added tris(dibenzylideneacetone) dipalladium(0) (0.1 g, 0.108 mmol), 2,2′-bis(diphenylphosphino)-1-1″-binaphthalene (0.12 g, 0.216 mmol) and t-BuONa (0.21 g, 2.16 mmol) and the reaction mixture was stirred at 100° C. for 2 h. The mixture was concentrated under reduced pressure and the crude material was purified by silica gel chromatography to afford 280 mg (36%) of the title compound as a white solid. 1H NMR (400 MHz, CDCl3) δ 7.45 (d, 1H), 7.15 (m, 3H), 6.19 (s, 2H), 3.82 (s, 3H), 3.71 (m, 8H), 3.26 (s, 3H), 2.79 (t, J=6.6 Hz, 2H), 1.87 (m, 2H); MS (ESI+) m/z=353 (M+H).
Sample 13 was prepared as shown below in Scheme 3. Compound numbers used in this Example pertain only to this Example; Sample 13 is referred to elsewhere as compound 13.
To a solution of lithium diisopropylamide (31.6 mmol) in 50 mL of THF at −78° C. was added 3-bromothiophene (3.0 g, 31 mmol) (1). After stirring for 1 h, formylpiperidine (3.50 g, 31.6 mmol) (2) was added and the reaction was warmed to 0° C. After 12 h, the reaction mixture was partitioned between DCM and sat. NH4Cl and the organic layer was separated, dried over MgSO4, filtered, and concentrated under reduced pressure. The crude material was purified by silica gel chromatography to afford 1.90 g (54%) of the title compound as a yellow oil. 1H NMR (400 MHz, CDCl3) δ 9.99 (s, 1H), 7.71 (d, J=5.2 Hz, 1H), 7.15 (d, J=4.8 Hz, 1H).
A solution of 3-bromothiophene-2-carbaldehyde (3) (1.90 g, 0.99 mmol) and sodium azide (3.23 g, 50 mmol) in 50 mL of 1,3-dimethyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinone (DMPU) was heated to 50° C. and stirred for 36 h. The reaction mixture was poured onto ice water and then extracted with DCM. The organic extract was dried over Na2SO4, filtered, and concentrated under reduced pressure. The crude material was purified by silica gel chromatography to afford 1.00 g (66%) of the title compound as a yellow oil. 1H NMR (400 MHz, CDCl3) δ 9.93 (s, 1H), 7.72 (d, J=5.2 Hz, 1H), 7.04 (d, J=5.2 Hz, 1H).
To a mixture of 3-azidothiophene-2-carbaldehyde (4) (0.80 g, 5.22 mmol) and 1-(3,4,5-trimethoxyphenyl)ethanone (5) (1.64 g, 7.84 mmol) in MeOH (5 mL) was added a solution of NaOH (0.62 g, 15.66 mmol) in water (2 mL) and the reaction mixture was stirred at rt for 16 h. The reaction mixture was diluted with water and extracted with DCM. The organic extract was dried over Na2SO4, filtered and concentrated under reduced pressure. The crude material was purified by silica gel chromatography to afford 1.10 g (61%) of the title compound as a yellow solid. 1H NMR (400 MHz, CDCl3) δ 7.88 (d, J=15.6 Hz, 1H), 7.42 (d, J=5.2 Hz, 1H), 7.21 (d, J=15.2 Hz, 3H), 7.00 (d, J=5.2 Hz, 1H), 3.94 (d, J=5.2 Hz, 9H); MS (ESI+) m/z=346 (M+H).
A solution of (E)-3-(3-azidothiophen-2-yl)-1-(3,4,5-trimethoxyphenyl)prop-2-en-1-one (6) (1.20 g, 3.48 mmol) in 10 mL of xylenes was stirred at 150° C. for 30 min. The reaction mixture was concentrated under reduced pressure and the crude material was purified by silica gel chromatography to afford 1.00 g (91%) of the title compound as an off-white solid. 1H NMR (300 MHz, CDCl3) δ 9.48 (bs, 1H), 7.42 (d, J=5.4 Hz, 1H), 7.20 (s, 2H), 7.11 (s, 1H), 7.01 (d, J=5.7 Hz, 1H), 3.94 (d, J=1.8 Hz, 9H); MS (ESI+) m/z=318 (M+H).
A mixture of (4H-thieno[3,2-b]pyrrol-5-yl)(3,4,5-trimethoxyphenyl)methanone (7) (0.80 g; 2.52 mmol) and sodium borohydride (480 mg, 12.7 mmol) in 2-propanol (10 mL) was stirred in a sealed tube at 100° C. for 2 h. The reaction mixture was cooled and diluted with water and extracted with DCM. The organic extract was dried over Na2SO4 and concentrated. The crude material was purified by silica gel chromatography to afford 280 mg (37%) of the title compound as a white solid. 1H NMR (300 MHz, CDCl3) δ 7.92 (bs, 1H), 6.99 (d, J=5.1 Hz, 1H), 6.86 (d, J=5.1 Hz, 1H), 6.46 (s, 2H), 6.26 (s, 1H), 4.02 (s, 2H), 3.83 (t, J=3.75 Hz, 9H); MS (ESI+) m/z=304 (M+H).
Sample 15 was prepared as shown below in Scheme 4. Compound numbers used in this Example pertain only to this Example; Sample 15 is referred to elsewhere as compound 15.
To a solution of 6-(trifluoromethyl)benzothiazol-2-amine (1) (2.00 g, 9.17 mmol) in 20 mL of THF was added isoamyl nitrite (3.22 g, 27.5 mmol). The mixture was heated to reflux for 30 min, quenched with water, and extracted with ethyl acetate. The organic extract was dried over Na2SO4 and concentrated under reduced pressure. The crude material was purified by silica gel chromatography to afford 860 mg (46%) of the title compound as a yellow solid. 1H NMR (400 MHz, CDCl3) δ 9.16 (s, 1H), 8.26 (m, 2H), 7.78 (d, J=1.2 Hz, 1H).
A solution of 6-(trifluoromethyl)benzo[d]thiazole (3) (830 mg, 4.08 mmol) and hydrazine monohydrate (1.52 g, 30.6 mmol) in ethanol (20 mL) was heated to reflux for 1.5 h. The mixture was added to a solution of acetic acid (3 mL) in water (100 mL) and extracted with DCM. The organic extract was dried over Na2SO4 and concentrated under reduced pressure. The crude material was purified by silica gel chromatography to afford 670 mg (84%) of the title compound as a yellow oil. 1H NMR (400 MHz, CDCl3) δ 7.62 (d, J=1.2 Hz, 1H), 7.39 (dd, J=8.4, 2.0 Hz, 1H), 6.75 (d, J=8.4 Hz, 1H), 4.49 (s, 2H), 2.95 (s, 1H).
2-amino-5-(trifluoromethyl)benzenethiol (4) (400 mg, 2.07 mmol), 2-(3,4-dimethoxyphenyl) acetic acid (5) and Lawesson's reagent (0.29 g, 0.72 mmol) in a sealed tube was subjected to microwave heating at 190° C. for 5 min. The crude material was purified by silica gel chromatography to afford 320 mg (43%) of the title compound as a white solid. 1H NMR (400 MHz, CDCl3) δ 8.08 (s, 1H), 8.06 (s, 1H), 7.69 (d, J=7.5 Hz, 1H), 6.91 (m, 3H), 4.40 (s, 1H), 3.87 (d, J=6.0 Hz, 6H); MS (ESI+) m/z=354 (M+H).
Sample 17 was prepared as shown below in Scheme 5. Compound numbers used in this Example pertain only to this Example; Sample 17 is referred to elsewhere as compound 17.
A mixture of 2-chloro-1H-benzo[d]imidazole (1) (0.40 g, 2.63 mmol), potassium dihydrogenphosphate (0.36 g, 2.63 mmol) and 3,4,5-trimethoxyaniline (0.48 g, 2.63 mmol) in 20 mL of n-BuOH was stirred at 90° C. for 16 h. The reaction mixture was diluted with water and extracted with ethyl acetate. The organic extract was dried over Na2SO4 and concentrated under reduced pressure. The crude material was purified by silica gel chromatography to afford 0.43 g (54%) of the title compound as an off-white solid. 1H NMR (300 MHz, CDCl3) δ 10.8 (s, 1H), 9.29 (s, 1H), 7.28 (bs, 2H), 7.12 (s, 2H), 6.97 (bs, 2H), 3.79 (s, 6H), 3.62 (s, 3H); MS (ESI+) m/z=300 (M+H).
Sample 24 was prepared as shown below in Scheme 6. Compound numbers used in this Example pertain only to this Example; Sample 24 is referred to elsewhere as compound 24.
To a solution of tert-butyl thiophen-2-ylcarbamate (1) (0.50 g, 2.51 mmol) in 10 mL of DCM was added 2,2,2-trifluoroacetic acid (1.43 g, 12.55 mmol). The mixture was stirred at rt for 2 h. The reaction mixture was concentrated under reduced pressure to afford ˜0.50 g of the title compound, which was directly used for the next step without purification.
To an ice-cold solution of 2-(3,4,5-trimethoxyphenyl)acetic acid (3) (0.40 g, 1.77 mmol) in 10 mL of DCM was added oxalyl chloride (0.67 g, 5.30 mmol) followed by 1 drop of DMF. The mixture was stirred from 0° C. to rt for 2 h. The reaction mixture was concentrated under reduced pressure to afford ˜0.50 g of the title compound, which was used in the next step without purification.
To an ice-cold solution of thiophen-2-amine-TFA salt (2) (˜0.50 g, crude) and diisopropylethylamine (DIPEA) (0.99 g, 7.65 mmol) in 10 mL of DCM was added a solution of 2-(3,4,5-trimethoxyphenyl)acetyl chloride (4) (˜0.50 g, crude) in 5 mL of DCM and the reaction mixture was allowed to warm from 0° C. to rt over 2 h with stirring. The reaction mixture was diluted with water and extracted with DCM. The organic extract was dried over Na2SO4 and concentrated under reduced pressure. The crude material was purified by silica gel chromatography to afford 0.25 g of the title compound as a white solid. 1H NMR (300 MHz, CDCl3) δ 7.80 (bs, 1H), 6.87 (m, 1H), 6.81 (m, 1H), 6.56 (m, 1H), 6.51 (s, 2H), 3.86 (s, 9H), 3.70 (s, 2H); MS (ESI+) m/z=308 (M+H).
As a starting point for the development of the presently disclosed compounds, a group of 47 compounds belonging to a series of 1-aryl-1,2,3,4-tetrahydro-β-carboline derivatives (
Conformational Analysis
Conformational analysis of all compounds in the series was carried out in order to determine the spatial position of their substituents and to find among the lowest energy conformers, their putative bioactive conformations.
Molecular modeling studies were performed using SYBYL software version 6.92. Three dimensional models of all compounds were built from a standard fragments library, and their geometry was subsequently optimized using the Tripos force field including the electrostatic term calculated from Gasteiger and Hückel atomic charges. The method of Powell available in the Maximin2 procedure was used for energy minimization until the gradient value was smaller than 0.0001 kcal/mol A2.
For each compound, a conformational search using the random search process as implemented in SYBYL was performed to identify its lowest energy conformations. Random conformational searching is a technique to locate energy minima of a molecule. It involves making random torsion changes to selected bonds, followed by a minimization. The cycle of random changes and minimization is repeated many times. After each cycle the new conformation is compared against all others found so far to see if it is unique. For the random search, the main options used are the maximum hits (n=6) which defined the minimum number of times each conformation must be found to stop searching for new conformations, the RMS threshold (RMS=0.2 Å) which defined the maximum RMS difference between two conformations before they are considered different.
The conformations produced by the random conformational search are fully optimized and can be used immediately for their geometry reoptimization with the semi-empirical MOPAC package version 6.0 using the Hamiltonian AM1 (keywords: PRECISE, NOMM, PARASOK), and Coulson partial atomic charges were calculated using the same method. Table 1 shows conformational spaces for selected compounds.
Leflunomide, but not its active metabolite, A771726, activates the AhR (Scheme 7). SGA 360 is a SAhRM that exhibits anti-inflammatory properties in vivo.
Peripheral blood mononuclear cells (PBMC), isolated from 3 healthy subjects, were isolated by density gradient centrifugation (Lymphoprep; Nycomed Pharma) from 5 mL heparinized blood samples. PBMC were resuspended in RPMI 1640 supplemented with 10% fetal calf serum at a concentration of 1×106 cells/mL, cultured in 24-well culture plates (Falcon Plastic) and stimulated with medium alone or anti-CD3/anti-CD28 (Miltenyi Biotec) antibodies with or without 100, 200, or 400 nM of AhR binding compounds (02, 04, 13, 15, 17, or 24), dimethyl sulfoxide or 6-formylindolo[3,2-b]carbazole (Ficz, 200 nM). Total RNA was extracted from cells cultured for 24 hours. For RNA preparation, cells were lysed in 1 mL of guanidium thiocyanate buffer and subjected to phenol/chloroform extraction using TRizol reagent (Invitrogen). The sample obtained was quantitated by absorbance at 260 nm and complementary DNA (cDNA) was synthetized from 1 mg of total RNA. cDNA was amplified using the following conditions: denaturation for 1 minute at 95° C.; annealing for 30 seconds at 58° C. for IFN-γ, and 60° C. for β-actin; and followed by 30 seconds of extension at 72° C. Primer sequences were as follows: human IFN-γ, forward (SEQ ID NO. 1) 5′-TGGAGACCATCAAGGAAGAC-3′, reverse (SEQ ID NO.2) 5′-GCGTTGGACATTCAAGTCAG-3′. IL-22 was evaluated using commercially available TaqMan probes (Applied Biosystems). β-actin (forward (SEQ ID NO. 3) 5′-AAGATGACCCAGATCATGTTTGAGACC-3′, reverse (SEQ ID NO. 4) 5′-AGCCAGTCCAGACGCAGGAT-3′) was used as a housekeeping gene. Gene expression was calculated using the ΔΔCt algorithm.
Using both in vitro and in vivo models of fibrosis, we have determined that AhR is a regulator of collagen synthesis in the gut, as described below.
Materials and Methods
Patients and Samples
Mucosal samples were taken from surgical specimens of 10 patients with fibrostenosing CD (median age, 37 years; range: 27-56 years); 7 of these 10 patients were receiving corticosteroids, and the remaining patients were on corticosteroids and azathioprine. Mucosal samples were also taken from 3 patients with ulcerative colitis (UC) undergoing colectomy for a chronic disease unresponsive to medical treatment and 6 UC patients undergoing endoscopy for recent flare-ups (median age, 38 years; range 29-5S years). Four UC patients were receiving corticosteroids while the remaining were treated with mesalazine. Normal controls included samples taken from 4 patients with irritable bowel syndrome and from macroscopically and microscopically unaffected areas of 6 patients undergoing colectomy for colon cancer (median age, 49 years; range 33-68 years).
Isolation and Culture of Intestinal Fibroblasts
All reagents were purchased from Sigma-Aldrich (Milan, Italy) unless specified. Intestinal fibroblasts were isolated and phenotypically characterized as described elsewhere. In all experiments, fibroblasts were used between passages 3 and 8. To examine whether AhR regulates collagen production, fibroblasts isolated from CD patients were starved overnight and then stimulated with TGF-β1 (TGF-β; 1 ng/mL; Peprotech EC, London, UK) or TNF-α (15 ng/mL; R&D Systems, Abingdon, UK) in the presence or absence of Ficz (final concentration, 100-400 nM; Alexis, Milan, Italy) or 2-methyl-2H-pyrazole-3-carboxylic acid (CH223191; final concentration 10 μM; Calbiochem, Nottingham, England), an AhR antagonist, for 24-48 hours. At the end, cells were used to extract RNA and cell-free supernatants were analyzed for collagen content.
Induction of Colonic Fibrosis
Trinitrobenzene sulfonic acid (TNBS) was dissolved in 45% ethanol and administered intrarectally to 8-week-old female balb/c mice for 7 weeks as previously described. Ficz (1 μg/mouse) or AhR antagonist (CH223191; 10 μg/mouse) was dissolved in phosphate-buffered saline (PBS) and given intraperitoneally every 48 hours after the fifth week of TNBS administration. Control mice were given PBS alone. Mice were examined 3 times a week for signs of colitis including weight loss and killed at week 8; afterwards tissues were collected for histology, RNA analysis and collagen analysis. Colonic sections were stained with H&E and with Masson's trichrome to detect connective deposition. Fibrosis was scored as mild, moderate, or severe as previously reported.
RNA Extraction, Complementary DNA Preparation and Real Time Polymerase Chain Reaction
RNA isolation, reverse transcription of the RNA and real-time PCR were carried out as previously described. RNA was extracted by using TRIzol reagent according to the manufacturer's instructions (Invitrogen, Carlsbad, CA). A constant amount of RNA (1 μg per sample) was reverse transcribed into complementary DNA, and this was amplified using the following conditions: denaturation for 1 minute at 95° C.; annealing for 30 seconds at 60° C. for human collagen I (Col1A1), human Col3A1, human alpha smooth muscle actin (α-SMA), and (3-actin, at 58° C. for human AhR and mouse Col1A2, followed by 30 seconds of extension at 72° C. Primer sequences were as follows: human Col1A1 (SEQ ID NO. 5) 5′-GGACACAGAGGTTTCAGTGG-3′, (SEQ ID NO. 5) 3′-GGTGACTTTGGAGACACAGG-5′; Col3A1 (SEQ ID NO. 6) 5′-GGAGAATGTTGTGCAGTTTGC-3′, (SEQ ID NO. 6) 3′-CGTTTGACGTGTTGTAAGAGG-5′; human α-SMA (SEQ ID NO. 7) 5′-TCTGGAGATGGTGTCACCCA-3′, (SEQ ID NO. 7) 3′-ACCCACTGTGGTAGAGGTCT-5′; human AhR (SEQ ID NO. 8) 5′-GAGCACAAATCAGAGACTGG-3′, (SEQ ID NO. 9) 5′-TGGAGGAAGCATAGAAGACC-3′; mouse Col1A2 (SEQ ID NO. 10) 5′-ACACAGTGGTATGGATGGAC-3′, (SEQ ID NO. 10) 3′-CAGGTAGGTATGGTGACACA-5′; 3-actin ((SEQ ID NO.3) 5′-AAGATGACCCAGATCATGTTTGAGACC-3′, (SEQ ID NO. 4) 5′-AGCCAGTCCAGACGCAGGAT-3′) was used as a housekeeping gene. Gene expression was calculated using the ΔΔCt algorithm.
Flow Cytometry
To assess the intracellular expression of AhR and the phosphorylated (p) form of p38, Erk1/2, NF-kB/p65 and Smad2/3, cells were fixed with 1% formaldehyde for 20 minutes and subsequently permeabilized with 0.5% saponin in 1% bovine serum albumin and stained with anti-AhR (1:50, final dilution; Abcam, Cambridge, UK), anti-p-p38 (pT180/pY182)-PE (final dilution 1:50; BD Biosciences, San Jose, CA), anti-p-ERK1/2 (pT202/pY204; pT184/pY186)-PE (final dilution 1:50; BD Biosciences), anti-p-NF-kB/p65-FITC (1:50 final dilution; eBioscience, San Diego, CA), and anti-p-Smad2/3 (1:50 final dilution; Cell Signaling, Danvers, MA). Appropriate secondary antibody and isotype-matched controls (BD Biosciences) were included in all experiments. Cells were analyzed using a FACS Verseflow cytometer and FACS Suite software (BD Biosciences).
Collagen Assay
Total collagen was measured in fibroblasts-free supernatants and mouse tissue samples by Sircol Collagen Assay Kit (Biocolor Ltd, Belfast, UK) in accordance with the manufacturer's instructions.
Statistical Analysis
Differences between groups were compared using the Student's t-test.
Results
AhR Activation Negatively Regulates Collagen Production by Intestinal Fibroblasts
AhR RNA transcripts were constitutively expressed in fibroblasts isolated from the gut of patients with CD, patients with UC and normal controls with no significant differences among groups (
AhR Controls Map Kinase Activation in CD Fibroblasts
Activation of p38 and ERK1/2 MAP kinases has been involved in the TGF-β1 and TNF-α-driven collagen induction. Therefore, we next investigated whether the AhR-mediated control of collagen synthesis was associated with changes of this intracellular pathway. To this end, we monitored p38 and ERK1/2 activation by flow-cytometry using specific antibodies, which recognize the phosphorylated/active forms of these proteins.
In unstimulated conditions, the fractions of cells expressing p-p38 or p-ERK1/2 were not affected by Ficz while being significantly increased by CH223191 (
AhR Controls TNBS-Induced Intestinal Fibrosis in Mice
To translate these data in vivo, we used an experimental model of intestinal fibrosis induced in Balb/c mice by repeated, rectal administration of low-doses of TNBS. To determine whether AhR activation interferes with collagen synthesis and fibrosis development, mice were given intra-peritoneally either Ficz or CH223191 after the fifth week of TNBS administration (
Discussion
This study investigated the role of AhR in the control of intestinal fibrosis. AhR was constitutively expressed in intestinal fibroblasts isolated from FS of CD patients as well as in intestinal fibroblasts of UC patients and normal controls. Although treatment of CD fibroblasts with Ficz did not modify the basal expression of collagen, inhibition of AhR with CH223191 led to increased collagen production, suggesting that constitutive AhR activation in these cells types is essential to keep collagen synthesis in check. Fibroblasts isolated from sites of FS in CD have enhanced capacity to respond to pro-fibrotic cytokines by producing collagen. Since studies in other systems have shown that AhR negatively regulates intracellular pathways activated by pro-fibrotic cytokines, it was next evaluated whether AhR activation is involved in TGF-β and TNF-α induced collagen production. CD fibroblasts displayed a different capacity to synthesize collagen when treated with Ficz or CH223191. In particular, Ficz dose-dependently reduced collagen RNA and protein expression while inhibition of AhR was followed by enhanced collagen production in response to TGF-β and TNF-α. Interestingly however, even at the greatest doses used in our system, Ficz did not completely abolish cytokine-induced collagen synthesis, raising the possibility that AhR does not control all the cytokine-driven intracellular pathways that lead to collagen production. Indeed, analysis of such signals revealed that Ficz abrogated activation of both p38 and ERK1/2 without affecting activation of Smad2/3 and NF-kB in fibroblasts stimulated with TGF-β or TNF-α, respectively. The fact that Ficz-mediated abrogation of p38 and ERK1/2 activation was accompanied by a 60% reduction of cytokine-driven collagen synthesis advocates a major role for MAP kinases in the control of collagen production in CD fibroblasts. The lack of effect of both Ficz and CH223191 on Smad2/3 and NF-kB activation in response to TGF-β or TNF-α is noteworthy, as these findings indicate that AhR activation does not induce a state of global unresponsiveness in intestinal fibroblasts, perhaps explaining why Ficz or CH223191 did not affect proliferation and survival of these cells.
The AhR-mediated negative regulation of collagen production was supported by in vivo studies in mice showing that Ficz was effective for minimizing fibrosis associated with chronic long-term inflammation. In contrast, mice receiving CH223191 exhibited a more intense collagen deposition as compared to control mice. In these studies, treatment with both Ficz and CH223191 started at a time point (week 5) that is characterized by pathological accumulation of collagen in the colon of TNBS-treated mice. Therefore, it is unlikely that the AhR-mediated inhibition of collagen production is secondary to suppression of the ongoing colitis.
In conclusion, these results show that AhR activation negatively controls collagen synthesis in the gut. These novel findings suggest that AhR-related compounds could help prevent and/or revert FS in patients with CD.
The entire disclosure of each of the patent documents and scientific articles cited herein is incorporated by reference for all purposes.
The invention can be embodied in other specific forms with departing from the essential characteristics thereof. The foregoing embodiments therefore are to be considered illustrative rather than limiting on the invention described herein. The scope of the invention is indicated by the appended claims rather than by the foregoing description, and all changes that come within the meaning and range of equivalency of the claims are intended to be embraced therein.
This application is a continuation patent application of U.S. patent application Ser. No. 15/321,967, filed on Dec. 23, 2016, which application is the U.S. national stage application under 35 U.S.C. § 371 of International Application No. PCT/EP2015/064613, filed on Jun. 26, 2015, which claims priority to and the benefit of U.S. Provisional Application No. 62/017,959, filed on Jun. 27, 2014, and U.S. Provisional Application No. 62/056,054, filed on Sep. 26, 2014, the entire contents of each of which are incorporated by reference herein. The instant application contains a Sequence Listing which has been submitted electronically in XML format and is hereby incorporated by reference in its entirety. Said XML copy, created on Feb. 21, 2023, is named GIU-040C1_SL.xml and is 11,691 bytes in size.
| Number | Name | Date | Kind |
|---|---|---|---|
| 3345376 | Hester et al. | Oct 1967 | A |
| 6069150 | Spinelli et al. | May 2000 | A |
| 6436925 | Lubisch et al. | Aug 2002 | B1 |
| 6720331 | Yeh et al. | Apr 2004 | B2 |
| 7138529 | Erickson et al. | Nov 2006 | B2 |
| 7375131 | Callahan et al. | May 2008 | B2 |
| 7524878 | Whitehouse et al. | Apr 2009 | B2 |
| 7767689 | Moon et al. | Aug 2010 | B2 |
| 7884124 | Heffernan et al. | Feb 2011 | B2 |
| 7981925 | Velicelebi et al. | Jul 2011 | B2 |
| 8058271 | Green et al. | Nov 2011 | B2 |
| 8278306 | Green et al. | Oct 2012 | B2 |
| 8372860 | Moon et al. | Feb 2013 | B2 |
| 8372991 | Velicelebi et al. | Feb 2013 | B2 |
| 8440711 | Allegretti et al. | May 2013 | B2 |
| 9216180 | Gardner et al. | Dec 2015 | B2 |
| 9980947 | Labadie et al. | May 2018 | B2 |
| 20020151534 | Ries et al. | Oct 2002 | A1 |
| 20030040527 | Yeh et al. | Feb 2003 | A1 |
| 20030195240 | Kalindjian et al. | Oct 2003 | A1 |
| 20050282849 | Moon et al. | Dec 2005 | A1 |
| 20070249670 | Evans et al. | Oct 2007 | A1 |
| 20070254878 | Cao et al. | Nov 2007 | A1 |
| 20080103164 | Gudmundsson et al. | May 2008 | A1 |
| 20080200468 | Simpson et al. | Aug 2008 | A1 |
| 20080207741 | Aydt et al. | Aug 2008 | A1 |
| 20080249180 | Gobbi et al. | Oct 2008 | A1 |
| 20090053192 | Millan et al. | Feb 2009 | A1 |
| 20090163545 | Goldfarb | Jun 2009 | A1 |
| 20130171103 | Davis et al. | Jul 2013 | A1 |
| 20150005275 | Plas et al. | Jan 2015 | A1 |
| 20150119426 | Marugan et al. | Apr 2015 | A1 |
| 20150299218 | Ambroise et al. | Oct 2015 | A1 |
| 20160030389 | Duncan et al. | Feb 2016 | A1 |
| 20180021273 | O'Connor et al. | Jan 2018 | A1 |
| 20180193309 | Duncan et al. | Jul 2018 | A1 |
| Number | Date | Country |
|---|---|---|
| 103864779 | Jun 2014 | CN |
| 107880040 | Apr 2018 | CN |
| H03287586 | Dec 1991 | JP |
| 101453413 | Nov 2014 | KR |
| WO-1998045268 | Oct 1998 | WO |
| WO-2001025190 | Apr 2001 | WO |
| WO-2001087882 | Nov 2001 | WO |
| WO-2001098290 | Dec 2001 | WO |
| WO-2002062778 | Aug 2002 | WO |
| WO-2005089764 | Sep 2005 | WO |
| WO-2006058088 | Jun 2006 | WO |
| WO-2007087129 | Aug 2007 | WO |
| WO-2007100657 | Sep 2007 | WO |
| WO-2007118137 | Oct 2007 | WO |
| WO-2007087130 | Dec 2007 | WO |
| WO-2007087429 | Jun 2008 | WO |
| WO-2008110488 | Sep 2008 | WO |
| WO-2008111299 | Sep 2008 | WO |
| WO-2008111300 | Sep 2008 | WO |
| WO-2008127714 | Oct 2008 | WO |
| WO-2009073620 | Jun 2009 | WO |
| WO-2010138758 | Dec 2010 | WO |
| WO-2010148350 | Dec 2010 | WO |
| WO-2011150162 | Dec 2011 | WO |
| WO-2012088420 | Jun 2012 | WO |
| WO-2014008214 | Jan 2014 | WO |
| WO-2015162216 | Oct 2015 | WO |
| WO-2015197861 | Dec 2015 | WO |
| WO-2017073743 | May 2017 | WO |
| WO-2018110669 | Jun 2018 | WO |
| Entry |
|---|
| Kawashima et al., Chem Pharm Bull (1995) 43(5), pp. 783-787. Filed by applicant in U.S. Appl. No. 15/321,967. |
| “Palladium-Catalyzed Intramolecular Cyclization of Nitroalkenes: Synthesis of Thienopyrroles” European Journal of Organic Chemistry (2017), 2017(14), 1902-1910. |
| Abou-Gharbia et al., (1987) “Antipsychotic Activity of Substituted γ-Carbolines” J. Med. Chem. 30(10):1818-23. |
| Alam et al., (2010), 'Notch Signaling Drives IL-22 Secretion in CD4+ T Cells by Stimulating the Aryl Hydrocarbon Receptor,' Proc Natl Acad Sci USA, 107(13):5943-8. |
| Audia et al., “Potent, Selective Tetrahydro-β-carboline Antagonists of the Serotonin 2B (5HT2B) Contractile Receptor in the Rat Stomach Fundus” Journal of Medicinal Chemistry (1996), 39(14), 2773-2780. |
| CAS Registry No. 1002531-50-1; STN entry date: Feb. 10, 2008; chemical name: 2-Thiophenecarboxylic acid, 3-methyl-5-[[2-(3,4,5-trimethoxyphenyl)acetyl]amino]-, ethyl ester. |
| CAS Registry No. 1061690-71-8; STN entry date: Oct. 15, 2008; chemical name: 3-Thiophenecarboxylic acid, 5-ethyl-2-[[2-(3,4,5-trimethoxyphenyl]acetyllamino]-, ethyl ester. |
| CAS Registry No. 1286986-56-8; STN entry date: Apr. 28, 2011; chemical name: 3-Thiophenecarboxylic acid, 4-cyclopropyl-2-[[2-(3,4,5-trimethoxyphenyl)acetyl]amino]-, ethyl ester. |
| CAS Registry No. 1287113-22-7; STN entry date: Apr. 28, 2011; chemical name: Benzeneacetamide, N-(3-cyano-2-thienyl)-3,4,5-trimethoxy-. |
| CAS Registry No. 1828225-26-8; STN entry date: Dec. 13, 2015; chemical name: 3-Thiophenecarboxylic acid, 5-[[2-(3,4,5-trimethoxyphenyl)acetyl]amino]-. |
| CAS Registry No. 528525-03-3; STN entry date: Jun. 10, 2003; chemical name: 1H-Pyrido[3,4-b]indole, 2,3,4,9-tetrahydro-1-[3-methoxy-4-(phenylmethoxy)phenyl]-6-methyl-. |
| CAS Registry No. 529476-20-8; STN entry date: Jun. 12, 2003; chemical name: 1H-Pyrido[3,4-b]indole, 1-(3,4-dimethoxyphenyl)-2,3,4,9-tetrahydro-6-methyl-. |
| CAS Registry No. 529476-21-9; STN entry date: Jun. 12, 2003; chemical name: 1H-Pyrido[3,4-b]indole, 1-(3,4-dimethoxyphenyl)-6-ethoxy-2,3,4,9-tetrahydro-. |
| CAS Registry No. 529476-23-1; STN entry date: Jun. 12, 2003; chemical name: 1H-Pyrido[3,4-b]indole, 6-bromo-1-(3,4-dimethoxyphenyl)-2,3,4,9-tetrahydro-. |
| CAS Registry No. 529476-24-2; STN entry date: Jun. 12, 2003; chemical name: 1H-Pyrido[3,4-b]indole, 1-(3,4-dimethoxyphenyl)-6-fluoro-2,3,4,9-tetrahydro-. |
| CAS Registry No. 532924-61-1; STN entry date: Jun. 18, 2003; chemical name: 1H-Pyrido[3,4-b]indole, 2,3,4,9-tetrahydro-1-[3-methoxy-4-(2-methylpropoxy)phenyl]-6-methyl-. |
| CAS Registry No. 532924-81-5; STN entry date: Jun. 18, 2003; chemical name: 1H-Pyrido[3,4-b]indole, 2,3,4,9-tetrahydro-1-[3-methoxy-4-(3-methylbutoxy)phenyl]-6-methyl-. |
| CAS Registry No. 532924-91-7; STN entry date: Jun. 18, 2003; chemical name: 1H-Pyrido[3,4-b]indole, 2,3,4,9-tetrahydro-1-[3-methoxy-4-(2-phenylethoxy)phenyl]-6-methyl-. |
| CAS Registry No. 533864-22-1; STN entry date: Jun. 19, 2003; chemical name: Phenol, 2-methoxy-4-(2,3,4,9-tetrahydro-6-methyl-1H-pyrido[3,4-b]indol-1-yl)-. |
| Chauhan et al. “lodine-Catalyzed Metal-Free Oxidative Ring Opening of 1-Aryltetrahydro-?-carbolines: Facile Synthesisof C-2 Aroyl and Aryl Methanimino Indole Derivatives” European Journal of Organic Chemistry (2018), 2018(34), 4776-4786. |
| CHEMCATS file: CAS Registry No. 1003752-91-7; STN entry date: Feb. 15, 2008; chemical name: Benzeneacetamide, 3,4,5-trimethoxy-N-[4-(trifluoromethyl)phenyl]-. |
| CHEMCATS file: CAS Registry No. 1030235-12-1; STN entry date: Jun. 24, 2008; chemical name: 3-Thiophenecarboxylic acid, 4-ethyl-5-methyl-2-[[2-(3,4,5-trimethoxyphenyl)acetyl]amino]-, ethyl ester. |
| CHEMCATS file: CAS Registry No. 1059182-77-2; STN entry date: Oct. 10, 2008; chemical name: Benzeneacetamide, N-(3,4-dimethoxyphenyl)-4-(trifluoromethyl)-. |
| CHEMCATS file: CAS Registry No. 1180442-67-4; STN entry date: Sep. 4, 2009; chemical name: Benzeneacetamide, 3,4,5-trimethoxy-N-phenyl-. |
| CHEMCATS file: CAS Registry No. 1323740-17-5; STN entry date: Aug. 26, 2011; chemical name: Benzeneacetamide, 3,4,5-trimethoxy-N-4-pyridinyl-. |
| CHEMCATS file: CAS Registry No. 639790-82-2; STN entry date: Jan. 21, 2004; chemical name: Benzeneacetamide, 3,4-dimethoxy-N-[4-(trifluoromethyl)phenyl]-. |
| CHEMCATS file; CAS Registry No. 1043293-02-2; STN entry date: Aug. 24, 2008; chemical name: 2-Thiophenecarboxylic acid, 5-[[2-(3,4,5-trimethoxyphenyl)acetyl]amino]-, methyl ester. |
| CHEMCATS file; CAS Registry No. 2094381-45-8; STN entry date: May 2, 2017; chemical name: 3-Thiophenecarboxylic acid, 5-(trifluoromethyl)-2-[[2-(3,4,5-trimethoxyphenyl)acetyl]amino]- ethyl ester. |
| CHEMCATS file; CAS Registry No. 2104951-19-9; STN entry date: Jul. 30, 2017; chemical name: Ethanone, 1-[5-(phenylmethyl)-4H-thieno[3,2-b]pyrrol-2-yl]-. |
| CHEMCATS file; CAS Registry No. 2105082-48-0; STN entry date: Jul. 30, 2017; chemical name: 4H-Thieno[3,2-b]pyrrole-2-carboxylic acid, 5-(phenylmethyl)-. |
| CHEMCATS file; CAS Registry No. 2106222-37-9; STN entry date: Aug. 1, 2017; chemical name: 4H-Thieno[3,2-b]pyrrole-2-carboxylic acid, 5-(phenylmethyl)-, methyl ester. |
| CHEMCATS file; CAS Registry No. 2106711-61-7; STN entry date: Aug. 1, 2017; chemical name: 4H-Thieno[3,2-b]pyrrole-2-carboxaldehyde, 5-[(3-fluorophenyl)methyl]-. |
| CHEMCATS file; CAS Registry No. 2107477-24-5; STN entry date: Aug. 3, 2017; chemical name: 4H-Thieno[3,2-b]pyrrole-2-carbonitrile, 5-(phenylmethyl)-. |
| CHEMCATS file; CAS Registry No. 2110143-04-7; STN entry date: Aug. 8, 2017; chemical name: 4H-Thieno[3,2-b]pyrrole-2-carboxylic acid, 5-(phenylmethyl)-, ethyl ester. |
| CHEMCATS file; CAS Registry No. 2111665-32-6; STN entry date: Aug. 10, 2017; chemical name: 4H-Thieno[3,2-b]pyrrole-2-carboxaldehyde, 5-(phenylmethyl)-. |
| CHEMCATS file; CAS Registry No. 529476-26-4; STN entry date: Jun. 12, 2003; chemical name: 1H-Pyrido[3,4-b]indole, 1-(3,4-dimethoxyphenyl)-2,3,4,9-tetrahydro-6-(phenylmethoxy)-. |
| De, Surya K., et al. “Design, synthesis, and structure-activity relationship studies of thiophene-3-carboxamide derivatives as dual inhibitors of the c-Jun N-terminal kinase.” Bioorganic & medicinal chemistry 19.8 (2011): 2582-2588. |
| DeGraw JI et al., (1967), 'Substituted 1,2,3,4-tetrahydro-ß-Carbolines. II.,' J Med Chem, 10(1):127-8. |
| Denison and Nagy, (2003), 'Activation of the Aryl Hydrocarbon Receptor by Structurally Diverse Exogenous and Endogenous Chemicals,' Annu Rev Pharmacol Toxicol, 43:309-34. |
| Desroses et al., (2013) “A facile and efficient synthesis of tetrahydro-β-carbolines,” Tetrahedron Letters 54:3554-7. |
| Dong et al., “Study on the Pictet-Spengler Reaction of Tryptamine Hydrohalide with Aldehyde” Chinese Journ. of Synthetic Chemistry, Hecheng Huaxue (2009), 17(5), 544-547. |
| Fodor et al., “Comparative evaluation of a Pictet-Spengler protocol in microwave-assisted conversions of tryptamine with aryl- and carboxyaryl aldehydes: role of ring strain in cyclocondensation of the primarily formed carboxyaryl-substituted ß-carbolines” Monatshefte fuer Chemie (2013), 144(9), 1381-1387. |
| Gellis et al., “Preparation and antiprotozoal evaluation of promising ß-carboline alkaloids” Biomedicine & Pharmacotherapy (2012), 66(5), 339-347. |
| Gu et al., (2000) 'The PAS Superfamily: Sensors of Environmental and Developmental Signals,' Annu Rev Pharmacol Toxicol, 40:519-61. |
| Hadjaz F et al., (2008), 'A Mild and Efficient Route to 2-Benzyl Tryptamine Derivatives via Ring- Opening of β-Carbolines,' Tetrahedron, 64(42):10004-8. |
| Hadjaz F et al., (2011), 'Antioxydant Activity of β-Carboline Derivatives in the LDL Oxidation Model,' Eur J Med Chem, 46(6):2575-85. |
| International Search Report for International Application No. PCT/EP2015/064613, mailed Nov. 25, 2015 (8 pages). |
| Ivanov et al., “A simple method for the synthesis of 1-substituted ß-carboline derivatives from tryptamine and carboxylic acids in polyphosphoric acid” Heterocycles (2005), 65(10), 2483-2492. |
| Jiang et al., “Furoyl and Benzofuroyl Pyrroloquinolones as Potent and Selective PDE5 Inhibitors for Treatment of Erectile Dysfunction” Journal of Medicinal Chemistry (2003), 46(3), 441-444. |
| Kawashima et al., “Synthesis and Pharmacological Evaluation of 1,2,3,4-Tetrahydro-β-carboline Derivatives” Chemical & Pharmaceutical Bulletin (1995), 43(5),783-7. |
| King, (1994) “Bioisosteres, Conformational Restriction, and Pro-drugs—Case History: An Example of a Conformational Restriction Approach,” Med. Chem., Principle and Practice 206-208. |
| Kranich, et al., (2007) “Rational Design of Novel, Potent Small Molecule Pan-Selectiv Antagonists,” J. Med. Chem, 50(6):1101-15. |
| Lindh et al. “Toward a Benchmarking Data Set Able to Evaluate Ligand—and Structure-based Virtual Screening Using Public HTS Data”, Journal of Chemical Information and Modeling, (2015), 55(2), 343-353. |
| Lipka et al., (2012) “Analytical and Preparative Chiral Separation of ß-Carboline Derivatives, LDL Oxidation Inhibitors, Using HPLC and CE Methodologies: Determination of Enantiomeric Purity,” Chromatographia 75:337-45. |
| Marafini, I. et al., “NPD-0414-2 and NPD-0414-24, Two Chemical Entities Designed as Aryl Hydrocarbon Receptor (AhR) Ligands, Inhibit Gut Inflammatory Signals.” Front. Pharmacol. 2019, 10, 380. |
| Monteleone et al., (2011), 'Aryl Hydrocarbon Receptor-Induced Signals Up-Regulate IL-22 Production and Inhibit Inflammation in the Gastrointestinal Tract,' Gastroenterology, 141(1):237-48. |
| Murray et al., (2010), 'Development of a Selective Modulator of Aryl Hydrocarbon (Ah) Receptor Activity that Exhibits Anti-Inflammatory Properties,' Chem Res Toxicol, 23(5):955-66 (NIH Public Access Author Manuscript). |
| Muscia et al., “Ultrasound Assisted Pictet-Spengler Synthesis of Tetrahydro-ß-Carboline Derivatives” Journal of Heterocyclic Chemistry (2016), 53(2),647-650. |
| Natarajan et al. (2003) “Highly efficient Lewis acid-catalysed Pictet-Spengler reactions discovered by parallel screening” Chem Commun., 7:916-17. |
| Negishi et al., (2005), 'Effects of Aryl Hydrocarbon Receptor Signaling on the Modulation of TH1/TH2 Balance,' J Immunol, 175(11):7348-56. |
| Nguyen and Bradfield, (2008), 'The Search for Endogenous Activators of the Aryl Hydrocarbon Receptor,' Chem Res Toxicol, 21(1): 102-16 [NIH Public Access Author Manuscript]. |
| O'Donnell et al., (2010), 'The Anti-Inflammatory Drug Leflunomide is an Agonist of the Aryl Hydrocarbon Receptor,' PLoS One, 5(10):e13128 (13 pages). |
| Peng et al., “Design, synthesis and antidiabetic activity of novel tetrahydrocarboline PPAR regulators,” Acta Pharmaceutica Sinica, Yaoxue Xuebao (2014), 49(4), 490-496. |
| Quintana et al., (2012), 'Aiolos Promotes TH17 Differentiation by Directly Silencing ll2 Expression,' Nat Immunol, 13(8):770-7 [NIH Public Access Author Manuscript]. |
| Rannug et al., (1995), 'Structure Elucidation of Two Tryptophan-Derived, High Affinity Ah Receptor Ligands,' Chem Biol, 2(12):841-5. |
| Rieder, et al., Animal Models of Intestinal Fibrosis: New Tools for the Understanding of Pathogenesis and Therapy of Human Disease. American Journal of Physciology, Oct. 1, 2012; 303(7): G786-G801. Published online Aug. 9, 2012; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4073977/?report=printable. |
| Rodriguez-Sosa et al., (2005), 'Over-Production of IFN-γ and IL-12 in AhR-Null Mice,' FEBS Lett, 579(28):6403-10. |
| Shen et al., “The preparation and evaluation of 1-substituted 1,2,3,4-tetrahydro- and 3,4-dihydro-ß-carboline derivatives as potential antitumor agents,” Chemical & Pharmaceutical Bulletin (2005), 53(1), 32-36. |
| Skinner et al., “Synthesis of 1-Aryl Substituted 9H-pyrido[3,4-b]indoles” Canadian Journal of Chemistry (1965), 43(8), 2251-3. |
| Somei M et al., (2001), 'The Chemistry of Indoles. CVII. A Novel Synthesis of 3,4,5,6,-tetrahydro-7-hydroxy-1H-azepino[5,4,3-cd]Indoles and a New Finding on Pictet-Spengler Reaction,' Chem Pharm Bull (Tokyo), 49(9):1159-65. |
| Spindler et al. “Synthesis and Investigation of Tetrahydro-ß-carboline Derivatives as Inhibitors of the Breast Cancer Resistance Protein (ABCG2)” Journal of Medicinal Chemistry (2016), 59(13), 6121-6135. |
| Stockringer B et al., (2011), 'External Influences on the Immune System via Activation of the Aryl Hydrocarbon Receptor,' Semin Immunol, 23(2):99-105. |
| Sugimoto et al., (2008), ‘IL-22 Ameliorates Intestinal Inflammation in a Mouse Model of Ulcerative Colitis,’ J Clin Invest, 118(2):534-44. |
| Sui et al., “Pyrimidinylpyrroloquinolones as Highly Potent and Selective PDE5 Inhibitors for Treatment of Erectile Dysfunction” Journal of Medicinal Chemistry (2002), 45(19), 4094-4096. |
| Sui et al., “Synthesis and biological activities of novel ß-Carbolines as PDE5 Inhibitors” Bioorganic & Medicinal Chemistry Letters (2003), 13(4), 761-765. |
| Tailleux A et al., (2005), ‘Increased Susceptibility of Low-Density Lipoprotein to ex vivo Oxidation in Mice Transgenic for Human Apolipoprotein B Treated with 1 Melatonin-Related Compound is not Associated with Atherosclerosis Progression,’ J Cardiovasc Pharmacol, 46(3):241-9. |
| Trifari et al., (2009), 'Identification of a Human Helper T Cell Population that has Abundant Production of Interleukin 22 and is Distinct from T(H)-17, T(H) 1 and T(H)2 Cells,' Nat Immunol, 10(8):864-71. |
| Veldhoen et al., (2008), ‘The Aryl Hydrocarbon Receptor Links TH17-Cell-Mediated Autoimmunity to Environmental Toxins,’ Nature, 453(7191):106-9. |
| Written Opinion of the International Searching Authority for International Application No. PCT/EP2015/064613, mailed Nov. 25, 2015 (9 pages). |
| Number | Date | Country | |
|---|---|---|---|
| 20230234963 A1 | Jul 2023 | US |
| Number | Date | Country | |
|---|---|---|---|
| 62056054 | Sep 2014 | US | |
| 62017959 | Jun 2014 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 15321967 | US | |
| Child | 17859505 | US |
