Assay apparatus and sensor holding method

Abstract

A sensor unit is retained on an assay stage of a surface plasmon resonance (SPR) assay apparatus. The sensor unit includes a prism having a sensing surface, disposed on an upper surface thereof, for detecting reaction of a sample. A flow cell is secured to the upper surface, and has a flow channel for flow of sample fluid containing the sample to the sensing surface. The sensor unit is set removably on the assay apparatus for optically measuring reaction of the sample. In sensor holding, the sensor unit is set on a stage surface of the assay stage by directing down the prism. In a pushing step, a lower surface of the prism is pushed on the stage surface in a first direction upright relative to the stage surface. Two holders push an upper face of the first and second ridges of the prism in the first direction.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

The above objects and advantages of the present invention will become more apparent from the following detailed description when read in connection with the accompanying drawings, in which:

FIG. 1 is a perspective view illustrating a surface plasmon resonance (SPR) assay method;

FIG. 2 is an explanatory view in perspective illustrating a sensor unit;

FIG. 3 is a front elevation illustrating the sensor unit;

FIG. 4 is a perspective view illustrating an assay stage;

FIG. 5 is a side elevation illustrating a flow cell holder in an upper position;

FIG. 6 is a side elevation illustrating the flow cell holder in a retaining position;

FIG. 7 is a perspective view illustrating a prism retaining mechanism;

FIG. 8 is an explanatory view illustrating the prism retaining mechanism in an upper initial position;

FIG. 9 is an explanatory view illustrating the prism retaining mechanism in a retaining position;

FIG. 10 is a flow chart illustrating holding and release of the sensor unit;

FIG. 11 is a perspective view illustrating a driving mechanism for a holder or pusher in which a cam plate is used;

FIG. 12 is a plan illustrating the driving mechanism; and

FIG. 13 is a side elevation illustrating the driving mechanism.

Claims

1. An assay apparatus in which a sensor unit is used, said sensor unit including an optical block having a sensing surface, disposed on an upper surface thereof, for detecting reaction of a sample, and a flow cell, secured to said upper surface, having a flow channel for flow of sample fluid containing said sample to said sensing surface, wherein light is applied to said optical block and received upon reflection thereon, for optically measuring reaction of said sample on said sensing surface, said assay apparatus comprising: an assay stage, having a stage surface for contacting a lower surface of said optical block, for supporting said sensor unit removably;an optical block retaining mechanism for retaining said sensor unit in a predetermined orientation on said stage surface by engagement with said optical block;said optical block retaining mechanism including a holder, shiftable between a retaining position and an initial position, for, when in said retaining position, pushing said lower surface of said optical block on said stage surface, and for, when in said initial position, releasing said optical block from retention on said stage surface, said holder exerting force of pushing to said optical block in a first direction which is upright relative to said stage surface.

2. An assay apparatus as defined in claim 1, further comprising a fluid dispenser having a pipette device; wherein said pipette device moves down in said first direction to access to said flow cell, and introduces said sample fluid when set at said flow channel.

3. An assay apparatus as defined in claim 2, wherein plural sensing surfaces are arranged in said sensor unit in a sensor longitudinal direction, plural flow channels are arranged in said sensor unit and correspond to said sensing surfaces, and plural pipette devices of said fluid dispenser access to said flow channels.

4. An assay apparatus as defined in claim 3, further comprising a biasing mechanism for biasing said holder toward said retaining position.

5. An assay apparatus as defined in claim 3, wherein said optical block includes first and second engageable portions formed to protrude from respectively lateral faces thereof; said holder is at least two holders so positioned that said stage surface is located between, for pushing an upper face of said first and second engageable portions in said first direction.

6. An assay apparatus as defined in claim 5, wherein said at least two holders are at least four holders arranged in first and second arrays extending in said sensor longitudinal direction thereof.

7. An assay apparatus as defined in claim 6, wherein said holders are shiftable independently from one another.

8. An assay apparatus as defined in claim 7, further comprising: a cam follower secured to said holder; anda cam surface for constituting a cam mechanism, and for driving said cam follower to shift said holder.

9. An assay apparatus as defined in claim 8, wherein said cam surface is so disposed that a clearance from said cam follower occurs when said holder reaches said retaining position.

10. An assay apparatus as defined in claim 3, wherein said optical block retaining mechanism further comprises a horizontal holder for pushing a first lateral face of said optical block in a second direction which is along said stage surface, to set a second surface of said optical block on a reference surface on said assay stage for holding in said second direction.

11. An assay apparatus as defined in claim 10, wherein said sensor unit moves in said sensor longitudinal direction for transfer to said assay stage, and said sensor longitudinal direction is crosswise to said first direction and to said second direction.

12. An assay apparatus as defined in claim 10, wherein said horizontal holder is supported on said holder, and starts retention in said second direction together with said holder.

13. An assay apparatus as defined in claim 3, further comprising a flow cell holder for accessing downwards to said flow cell of said sensor unit on said stage surface, and for pushing an upper surface thereof to press said flow cell on said optical block.

14. An assay apparatus as defined in claim 13, wherein pushing of said optical block retaining mechanism and said flow cell holder is started after transfer of said sensor unit to said assay stage is completed.

15. An assay apparatus as defined in claim 13, further comprising a cushion mechanism for reducing shock between said flow cell holder and said flow cell at a time of pushing.

16. A sensor holding method of retaining a sensor unit on an assay stage of an assay apparatus, said sensor unit including an optical block having a sensing surface, disposed on an upper surface thereof, for detecting reaction of a sample, and a flow cell, secured to said upper surface, having a flow channel for flow of sample fluid containing said sample to said sensing surface, said sensor unit being set removably on said assay apparatus for optically measuring reaction of said sample, said sensor holding method comprising: a setting step of setting said sensor unit on a stage surface of said assay stage by directing down said optical block; anda pushing step of pushing a lower surface of said optical block on said stage surface in a first direction which is upright relative to said stage surface.