ASSAY FOR SIMULTANEOUS DETECTION OF MULTIPLE HPV TYPES

Information

  • Research Project
  • 3498295
  • ApplicationId
    3498295
  • Core Project Number
    R43GM042273
  • Full Project Number
    1R43GM042273-01
  • Serial Number
    42273
  • FOA Number
  • Sub Project Id
  • Project Start Date
    3/10/1989 - 35 years ago
  • Project End Date
    12/1/1989 - 34 years ago
  • Program Officer Name
  • Budget Start Date
    3/10/1989 - 35 years ago
  • Budget End Date
    12/1/1989 - 34 years ago
  • Fiscal Year
    1989
  • Support Year
    1
  • Suffix
  • Award Notice Date
    3/9/1989 - 35 years ago
Organizations

ASSAY FOR SIMULTANEOUS DETECTION OF MULTIPLE HPV TYPES

The aim of this project is to develop a rapid, highly sensitive assay for simultaneously detecting the in situ hybridization of multiple human papilloma virus (HPV) type-specific DNA probes to the HPV DNA in a single specimen. In Phase I, we will be using a model system consisting of two well-characterized cervical caricinoma cell lines with known levels of HPV type 16 and 18 DNA. Hybridization of biotin- and DNP-labeled HPV type 16 and HPV type 18 DNA probes will be visualized by fluorescence microscopy following binding of the appropriate fluorescently labeled antibodies to the DNA probes. Various fluorescent labels and antibody preparations will be tested. Antibody blocking, binding, and washing steps will be optimized. The detection system which provides maximal signal intensity and specificity and minimal background will be used in subsequent Phase II studies in which the efficacy of their assay for identifying high and low risk HPV types in biopsy specimens will be assessed. These studies will result in a simple, clinically relevant HPV identification assay which is capable of supplying a considerable amount of critical information with a minimum of effort, time and sample material.

IC Name
NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
  • Activity
    R43
  • Administering IC
    GM
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    821
  • Ed Inst. Type
  • Funding ICs
  • Funding Mechanism
  • Study Section
    SSS
  • Study Section Name
  • Organization Name
    ENZO BIOCHEM, INC.
  • Organization Department
  • Organization DUNS
  • Organization City
    NEW YORK
  • Organization State
    NY
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    10013
  • Organization District
    UNITED STATES