Patent Application
20190113527
The present invention relates to a diagnostically useful carrier comprising a means for capturing Neurogranin, a means for capturing BACE1 and preferably a means for capturing one or more additional biomarkers; a kit comprising the diagnostically useful carrier, optionally further comprising a means for detecting Neurogranin, a means for detecting BACE1 and preferably a means for detecting the one or more additional biomarkers; and a method comprising the steps contacting a sample from a subject with a means for capturing Neurogranin, isolating the means for capturing Neurogranin from the sample and contacting a sample from a subject with a means for capturing BACE1 and isolating the means for capturing BACE1 from the sample.
Developing diagnostic systems for neurological diseases is a continuing challenge in biomedical science, not in the least because many neurological symptoms encountered may be accounted for by a huge variety of causes including genetically-inherited diseases, drug abuse, malnutrition, infection, injury, psychiatric illness, immunological defects and cancer.
Since a neurological disease is rarely associated with a unique pattern of clinical symptoms, it is often difficult to provide a reliable diagnosis based on the observation and clinical examination of the patients or based on the medical histories.
The importance of an early diagnosis cannot be overemphasized. Many neurological disorders, most prominently neurodegenerative diseases, such as Alzheimer's disease (AD) and Parkinson's disease (PD), cannot be cured at this moment, but drugs are available that may be used to slow down their progression. The earlier the diagnosis, the better the chances to exploit the spectrum of available drugs to the full benefit of the patient.
In addition, neurodegenerative diseases progress at strikingly different rates in individual patients. While the symptoms deteriorate within several months in some patients, others may go on to live with mild symptoms for years.
Any option to assess the future development may help patients, their medical doctors, relatives and care takers to adjust the treatment and the patients' lifestyles accordingly. This may also be a useful option for patients who are at elevated risk of developing AD or PD or psychiatric conditions such as depression.
Unfortunately, currently available diagnostic biomarkers do not allow for the prediction of the future progression of the disease. In particular, tau and Aβ are state markers with limited prognostic value regarding future cognitive deterioration. Tangles and plaques are only weakly correlated with cognitive impairment.
The state of the art discloses a range of assays. EP 1 774 023 discloses a method to diagnose the occurrence of brain damage based on the detection of full-length Neurogranin in various types of samples, but does not disclose an assay detecting BACE1 in cerebrospinal fluid (CSF) in addition.
Barao et al. (2013) disclose a sandwich ELISA for the detection of BACE1, but not Neurogranin. However, BACE1 is referred to as a “poor diagnostic marker for AD”, with “very small” differences between samples from AD patients and control groups. Clearly, there is the need to develop diagnostic assays that can be used to predict the onset or future course of such diseases.
Therefore, the problem underlying the present invention is to provide a diagnostic assay that may be used to diagnose neurodegenerative diseases, more specifically AD.
In particular, such assay should allow to diagnose patients having specific and/or unspecific symptoms that may be associated both with neurodegenerative and other neurological diseases or psychiatric diseases such as depression.
Another problem underlying the present invention is to provide a diagnostic assay that allows the prediction of the future progression of neurodegenerative diseases, more specifically AD.
Another problem underlying the present invention is to provide an assay that may be used for the diagnosis of psychiatric diseases, more specifically depression.
The problem underlying the present invention is solved by the subject-matter of the attached independent and dependent claims.
In a first aspect, the problem underlying the present invention is solved by a diagnostically useful carrier comprising a means for capturing Neurogranin, a means for capturing BACE1 and preferably a means for capturing one or more additional biomarkers.
In a second aspect, the problem underlying the present invention is solved by a kit comprising the diagnostically useful carrier according to the present invention, optionally further comprising a means for detecting Neurogranin, a means for detecting BACE1 and preferably a means for detecting the one or more additional biomarkers.
In a preferred embodiment, the means for specifically capturing Neurogranin and the means for specifically capturing BACE1 are spatially separated.
In another preferred embodiment, the diagnostically useful carrier is selected from the group comprising a bead, a test strip, a microtiter plate, a blot, preferably from the group comprising western blot, line blot and dot blot, a glass surface, a slide, a biochip, a membrane, a microarray, an electrophoresis gel and a submicrometer sized particle
and is more preferably either
a microtiter plate having a well comprising the means for specifically capturing Neurogranin, having a well comprising the means for specifically capturing BACE1 and optionally having one or more additional wells, each comprising a means for specifically capturing the one or more additional biomarkers
or
a bead comprising the means for specifically capturing Neurogranin and a bead comprising the means for specifically capturing BACE1 and, optionally, one or more additional beads, each comprising a means for specifically capturing the one or more additional biomarkers,
most preferably said microtiter plate.
In a third aspect, the problem underlying the present invention is solved by a method comprising the steps
In another preferred embodiment, the method further comprises the steps
In another preferred embodiment, the method further comprises the steps
In another preferred embodiment, the method further comprises the steps
In a 4th aspect, the problem underlying the present invention is solved by a diagnostically useful carrier obtainable by the method according to the present invention.
In another preferred embodiment, the one or more additional biomarkers are selected from the group comprising Abeta42 and -40, P-tau, T-tau and neurofilament protein.
In another preferred embodiment, the carrier, kit or method is adapted for detecting Neurogranin, BACE1 and optionally one or more additional biomarkers using a method from the group comprising immunodiffusion techniques; immunoelectrophoretic techniques; light scattering immunoassays; agglutination techniques; labeled immunoassays such as those from the group comprising radiolabeled immunoassay, enzyme immunoassays such as colourimetric assays, chemiluminscence immunoassays, fluorescence immunoassays, nanogold labeling; mass spectrometry; and Surface Plasmon Resonance, preferably enzyme immunoassay.
In another preferred embodiment, the carrier, kit or method is adapted for detecting Neurogranin, BACE1 and optionally one or more additional biomarkers based on a sandwich ELISA.
In a 5th aspect, the problem underlying the present invention is solved by a use of the diagnostically useful carrier or kit according to the present invention for diagnosing a neurological or neurodegenerative disease, preferably Alzheimer's disease (AD).
In another preferred embodiment, the diagnosis aims to predict the rate of future cognitive decline.
In another preferred embodiment, the diagnosis aims to distinguish a neurodegenerative disease, preferably mild AD, and depression with or without cognitive impairment.
In a 6th aspect, the problem underlying the present invention is solved by a diagnostically useful carrier or kit according to the present invention for diagnosing a depression, preferably in aged patients.
The invention relates to a diagnostically useful carrier, which is preferably a solid carrier made from an artificial material such as glass or plastic. This carrier is associated with a means for specifically capturing an antibody in a bodily fluid sample from a subject, preferably a mammalian subject, more preferably a human subject. In a preferred embodiment, the solid carrier is a diagnostic device, more preferably selected from the group comprising a bead, a test strip, a microtiter plate, a blot, preferably from the group comprising western blot, line blot and dot blot, a glass surface, a slide, a biochip, a membrane, a microarray, an electrophoresis gel and a submicrometer sized particle, more preferably either a microtiter plate or a bead, most preferably said microtiter plate. The diagnostic carrier may be a one-piece diagnostic carrier, for example a microtiter plate having more than one well, for example one well comprising a means for capturing Neurogranin and another well comprising a means for capturing BACE1, or it may comprise two or more pieces, each of which may be used for a separate assay. For example, the diagnostic device may comprise a first bead comprising a means for capturing Neurogranin and another bead comprising a means for capturing BACE1.
According to the present invention, the carrier comprises two or more means for specifically capturing a polypeptide, one means for capturing Neurogranin and one means for capturing BACE1, optionally one or more means for capturing one or more additional biomarkers such as one, two, three, four, five, ten, fifty, one hundred or one thousand more means, each for capturing an additional biomarker.
Said means is immobilized on said carrier such that the means is well placed to interact with and bind to the polypeptide to be captured such as Neurogranin, BACE1 or any additional biomarker when contacted with a liquid sample comprising said polypeptide to be captured to the effect that a complex comprising the means and the polypeptide to be captured is formed. The means may be any reagent binding specifically to Neurogranin, BACE1 or any additional biomarker and is preferably selected from the group comprising an antibody, preferably a monoclonal or polyclonal, more preferably a monoclonal antibody, and an aptamer, more preferably an antibody. Calmodulin or a variant thereof may be used as a means binding specifically to Neurogranin in addition to an antibody or an aptamer. The antibody is preferably an isolated and/or recombinant antibody. The person skilled in the art is familiar with methods that may be used to generate such a means.
Preferably, the means for capturing has a binding affinity KD to the molecule to be captured, preferably Neurogranin, BACE1 or any additional biomarker, of less, i.e. a value indicating stronger binding, than 1 mM, in order of increasing preference, 0.5 mM, 0.250 mM, 0.100 mM, 50 μM, 25 μM, 10 μM, 5 μM, 1 μM, 0.5 μM, 0.25 μM, 0.1 μM, 50 nM, 25 nM, 10 nM, 1 nM, 100 μM, 50 μM, 25 μM, 10 μM, 5 μM, 1 μM, 0.5 μM, 0.25 μM, 0.1 μM as measured using surface plasmon resonance in PBS at pH7 at 25° C.
In a preferred embodiment, the term “Neurogranin”, as used herein, refers to full-length mammalian, preferably human Neurogranin as represented by database entry Uniprot Q92686 or a fragment thereof, more preferably a C-terminally truncated fragment of said Neurogranin that comprises at least SEQ ID NO 4, and optionally additional N-terminal amino acid residues, but lacks the three C-terminal residues of full-length Neurogranin, more specifically S76, G77 and D78, which truncated form is also referred to as “neurogranin trunc p75” throughout this application. Neurogranin or the fragment thereof, as detected, may carry one ore more modifications including those selected from the group comprising a N-acetylation such as e.g. N-acetyl-Met1, a phosphorylation such as phospho-Ser36, and a citrullination such as for e.g. Arg68 based on the numbering of SEQ ID NO 1. Any database entry referred to throughout this document refers to the version and sequence in that database as on-line on the date of the application.
In a preferred embodiment, the term “BACE1”, as used herein, refers to full-length mammalian, preferably human BACE1 or a fragment thereof, preferably the fragment represented by SEQ ID NO 2. BACE1 or a fragment thereof, as detected, may carry one or more modifications including those selected from the group comprising an acetylation preferably of a lysine side chain selected from the group comprising Lys105, Lys254, Lys258, Lys264, Lys278, Lys279, and Lys286, a glycosylation preferably of an asparagine side chain selected from the group comprising Asn132, Asn151, Asn202 and Asn333, a disulfide bond preferably between a pair of cysteine side chains selected from the group comprising Cys195 and Cys399, Cys257 and Cys422 as well as Cys309 and Cys359. (All of the above amino acid numbering is based on SEQ ID NO 2.).
In a preferred embodiment, the one or more additional biomarkers are biomarkers that may be used to diagnose a neurodegenerative disease, more preferably AD, more preferably an AD symptom selected from psychiatric conditions, such as depression. The one or more additional biomarkers are preferably selected from the group comprising Abeta42 (Aβ1-42), preferably represented by SEQ ID NO 5, Abeta40 (Aβ1-40), preferably represented by SEQ ID NO 6, Tau as preferably represented by SEQ ID NO 7, either total tau, also referred to as t-tau, or tau comprising at least one phosphorylated sequence motif, also referred to as p-tau, and neurofilament protein, preferably as represented by SEQ ID NO 8. In a preferred embodiment, the term “t-tau”, as used herein, comprises the entirety of all tau molecules or fragments thereof. In another preferred embodiment, the term “p-tau”, as used herein, comprises the entirety of all phosphorylated tau molecules or fragments thereof.
According to the present invention, a means for detecting the captured polypeptide, such as Neurogranin, BACE1 or the one or more additional biomarkers, is required. In a preferred embodiment, a secondary antibody or aptamer, different or the same as the primary, capturing antibody or aptamer, binding to the captured polypeptide is used. The secondary antibody or aptamer may be associated with a label that is straightforward to detect, for example a fluorescent, radioactive or enzymatically active label, the latter of which may catalyze a chemiluminescent reaction or the generation of a molecule detectable using colourimetry or spectroscopy or another analytical method.
The means for detecting is preferably designed such that it binds the captured polypeptide and the latter may be detected whilst bound. For example, if a primary antibody is used to capture the polypeptide in a sandwich-like fashion, then the secondary antibody binds to another epitope which is still accessible while the polypeptide is bound to the first antibody.
According to the present invention, there is provided a first reaction mixture comprising the diagnostically useful carrier, a sample and a means for capturing Neurogranin, which means is optionally in complex with Neurogranin, and a second reaction mixture, which is in contact with the first reaction mixture or spatially separated, preferably spatially separated, comprising a diagnostically useful carrier, a sample and a means for capturing BACE1, which means is optionally in complex with BACE1. The Neurogranin in complex may be associated with a detectable ligand to Neurogranin. The BACE1 in complex may be associated with a detectable ligand to BACE1.
The inventive teachings provide a kit, preferably for diagnosing neurodegenerative diseases, more preferably for diagnosing AD. Such a kit is a container that comprises specific reagents required to practice the inventive method, in particular the diagnostically useful carrier according to the present invention, optionally one or more solutions required to practice the inventive method in addition, preferably selected from the group comprising sample dilution buffer, washing buffer and buffer comprising a means for detecting any captured polypeptide, such as a secondary antibody. Furthermore, it may comprise instructions detailing how to use the kit and the inventive diagnostically useful carrier for contacting the inventive polypeptide with a bodily fluid sample from a subject, preferably a human subject. Furthermore, the kit may comprise a positive control, for example recombinant Neurogranin and/or BACE1, known to bind to the means for capturing Neurogranin, BACE1 and, optionally, one or more additional biomarkers, and a negative control. Finally, such a kit may comprise one or more standard solutions for preparing a calibration curve, for example a standard solution comprising Neurogranin at a known concentration, a standard solution comprising BACE1 at a known concentration and, optionally, one or more standard solutions, each comprising an additional biomarker at a known concentration.
In a preferred embodiment, the term “detecting”, as used herein, means that the presence or absence of the polypeptide to be detected, preferably Neurogranin, BACE1 or the one or more additional biomarkers, may be determined. In a more preferred embodiment, the term refers to an at least semi-quantitative determination, allowing for a rough approximation of the concentration. In a most preferred embodiment, the term refers to determining the concentration of the polypeptide to be detected, either relative or absolute, such that the concentration ratio of Neurogranin and BACE1 and, optionally, the ratio of Neurogranin or BACE1 to one or more additional biomarkers, in the sample may be determined. For example, the unknown concentration of both Neurogranin and BACE1 in a sample may be determined by running an ELISA of the sample to be examined in parallel with known amounts of Neurogranin and of BACE1, respectively, in separate reactions, followed by establishing calibration curves each for Neurogranin and BACE1, based on the known amounts, and determining the first unknown concentration of Neurogranin and BACE1 in the sample. In a preferred embodiment, the method is selected from the group comprising method from the group comprising immunodiffusion techniques, immunoelectrophoretic techniques, light scattering immunoassays, agglutination techniques, labeled immunoassays such as those from the group comprising radiolabeled immunoassay, enzyme immunoassays such as colourimetric assays, chemiluminscence immunoassays, fluorescence immunoassays, nanogold labeling; mass spectrometry, and Surface Plasmon Resonance, preferably enzyme immunoassay. Such methods are described in the state of the art, for example in Fredolini et al. (2016) Immunocapture strategies in translational proteomics, Expt. Rev. Proteomics 13(1), 83-98.
In a preferred embodiment, the means for capturing Neurogranin, BACE1 and, optionally, the one or more additional biomarkers, are spatially separated, i.e. separated in different reaction compartments such that there is no transfer of reagents when then concentrations are determined. Alternatively, the reactions may be carried out in a one-pot manner provided there is the possibility to determine the ratio of Neurogranin, BACE1 and, optionally, the one or more additional biomarkers, for example by using different means for detecting not interfering with each other. In another embodiment, the two or more means for capturing may be separated following the capturing reaction, which is carried out in the same vessel, for the separate determination of the concentration of Neurogranin, BACE1 and of the one or more additional biomarkers. In another embodiment, the diagnostically useful carrier comprises two or more means for capturing, but is split up following the capturing reaction and reacts, in two spatially separate reactions, with two more different means for the detection of Neurogranin, BACE1 and, optionally, of one or more additional biomarkers. In a preferred embodiment, the same sample is subsequently or concomitantly subject to reactions for the detection of Neurogranin, BACE1 and, optionally, one or more additional biomarkers.
In a preferred embodiment, the diagnostically useful carrier is a bead, which is preferably a particle to which a means for capturing a biomarker may be attached such that the bead, together with such means, may be separated from a liquid solution, based on its sedimentation properties, more efficiently than the means for capturing the biomarker by itself, for example by decanting, centrifugation, filtrations, preferably decanting. Typically such bead is a bead made of a carbohydrate which is chemically inert in aqueous solution, for example an agarose or sepharose bead. The bead may be a magnetic bead which may comprise iron at a content of 5 to 30% (weight/weight). The bead may have a size of 0.1 to 10 μm, preferably 0.5 to 5 μm. The diagnostically useful carrier may comprise a bead coated with a means for capturing Neurogranin, preferably an antibody to Neurogranin, and a bead coated with a means for capturing BACE1, preferably an antibody to BACE1, and, optionally one or more beads coated with a means for capturing the one or more additional biomarkers, preferably an antibody binding to the additional biomarker for each bead, and, optionally, with a means for capturing the one or more additional biomarkers, preferably an antibody or aptamer, preferablay antibody to the one or more additional biomarkers.
In another preferred embodiment, the diagnostically useful device is a microtiter plate comprising a well coated with a means for specifically capturing Neurogranin, a well coated with a means for specifically capturing BACE1 and, optionally, one or more wells, each comprising a means for capturing an additional biomarker.
The sample tested according to the present invention is a blood sample, a brain tissue sample or a CSF sample, preferably a CSF sample, more preferably a CSF sample from a live patient, preferably suspected of suffering from or showing symptoms characteristic for a neurodegenerative disease, preferably initial symptoms of a neurodegenerative disease such as AD.
The present invention relates to a method comprising the steps detecting in a sample from a subject Neurogranin, BACE1 and, optionally, one or more additional biomarkers. Such a method may comprise the steps providing a sample from a subject, contacting the sample with the diagnostically useful carrier according to the present invention under conditions compatible with the formation of a complex comprising the diagnostically useful carrier and the polypeptides to be detected such as Neurogranin and BACE1, for example by simply immersing the diagnostically useful carrier with the sample or with diluted sample for a sufficient period of time. Another subsequent step may involve isolating any complex from the remainder of the sample, for example by gently removing sample from the carrier following formation of the complex such that the latter is preserved, for example by decanting. Subsequently, the diagnostically useful carrier may be washed, again under conditions that allow the components of the complex formed to remain associated. Finally, the complex may be detected, either by directly detecting the intact complex, for example by adding a detectable ligand binding to the complex, but not to the means for capturing by itself. Alternatively, the complex may be detected by dissociating the complex following removal of the sample, removing the polypeptide to be detected from the means for capturing and detecting any dissociated polypeptide. The method is preferably an in vitro method.
In a preferred embodiment, a detectable ligand is used to detect a polypeptide such as Neurogranin, BACE1 and, optionally, the one or more additional biomarkers. Preferably the ligand may have a binding affinity KD to the polypeptide to be detected of less, i.e. a value indicating stronger binding, than 1 mM, in order of increasing preference, 0.5 mM, 0.250 mM, 0.100 mM, 50 μM, 25 μM, 10 μM, 5 μM, 1 μM, 0.5 μM, 0.25 μM, 0.1 μM, 50 nM, 25 nM, 10 nM, 1 nM, 100 μM, 50 μM, 25 μM, 10 μM, 5 μM, 1 μM, 0.5 μM, 0.25 μM or 0.1 μM as measured using surface plasmon resonance in PBS at pH7 at 25° C. Most preferably the ligand has binding affinity KD to the polypeptide to be detected of 1 nM or less. In a preferred embodiment, the detectable ligand is preferably selected from the group comprising an antibody, preferably a monoclonal or polyclonal, more preferably a monoclonal antibody, or an aptamer, more preferably an antibody. The antibody is preferably an isolated and/or recombinant antibody. The detectable ligand may be associated with an artificial label, preferably from the group comprising an enzymatically active polypeptide, a radionucleotide, a spin label, a fluorescent label and a chromophor label.
In a preferred embodiment, the polypeptide to be detected, more specifically neurogranin and BACE1 and, optionally, one or more additional biomarkers, is detected using a sandwich ELISA. Briefly, the diagnostically useful carrier, preferably a microtiter plate or bead, comprises a first antibody or aptamer immobilized on the carrier that serves as the means for capturing the polypeptide to be detected. The complex comprising the first antibody or aptamer and the polypeptide to be detected is detected by addition of a second antibody or aptamer which carries a label and binds to an epitope the same or other than the epitope binding the first antibody. Various immunoassays for detecting neurogranin and BACE1 are described in the state of the art, for example in De Vos et al., 2015, Kvartsberg et al., 2015, Thorsell et al, 2010, WO2012/155134 and Barao et al., 2013. In a preferred embodiment, a sandwhich assay is used to detect neurogranin using a first monoclonal antibody as a capture antibody and a second monoclonal antibody as a detection antibody. In a more preferred embodiment the detection antibody binds to a neurogranin epitope closer to the C-terminus, preferably to an epitope of at least five amino acids, preferably at least 10 amino acids comprising SEQ ID NO 3 (AGGGP) than the epitope to which the capture antibody binds. Most preferably both antibodies bind to neurogranin epitopes located in the collagen-like domain of neurogranin as represented by SEQ ID NO 4.
In a preferred embodiment, the term “diagnosis”, as used herein, refers to any kind of procedure aiming to obtain information instrumental in the assessment of whether a patient suffers from or is likely or more likely than the average or a comparative subject, the latter preferably having similar symptoms, to suffer from a certain disease or condition in the past, at the time of the diagnosis or in the future, to find out how the disease is progressing or is likely to progress in the future or to evaluate the responsiveness of a patient with regard to a certain treatment, for example the administration of suitable drugs slowing down the progress of a neurodegenerative disease. In other words, the term “diagnosis” comprises not only diagnosing, but also prognosticating and/or monitoring the course of a disease or disorder. In a preferred embodiment, the disease or disorder is a neurological disease, more preferably a neurodegenerative disease, more preferably AD, and this may be distinguished from depression with or without cognitive impairment, preferably without cognitive impairment, using the teachings of the present invention. In people aged at least 40, more preferably 50, more preferably 60, more preferably 65, more preferably 70, more preferably 75, more preferably 80 years.
Therefore, the term “diagnosis” does preferably not imply that the diagnostic methods or agents according to the present invention will be definitive and sufficient to finalize the diagnosis on the basis of a single test, let alone parameter. Rather it may refer to a contribution to what is referred to as a “differential diagnosis”, i.e. a systematic diagnostic procedure considering the likelihood of a range of possible conditions on the basis of certain diagnostic parameters. This may involve diagnosing a disease or condition indirectly by way of ruling out another. The term “diagnosis” may also refer to a method or agent used to choose the most promising treatment regime for a patient. In other words, the method or agent may relate to selecting a treatment regimen for a subject.
According to the present invention, the diagnostic carrier or kit or method may be used to detect Neurogranin and BACE1 in a sample from a subject. In case such a subject is a patient suffering from mild cognitive impairment (MCI) due to Alzheimer's Disease, or from dementia due to Alzheimer's Disease, such as the patients described in the examples of this invention, the ratio of Neurogranin on BACE1 in the CSF of that subject may be used to assess or to monitor the rate of progression of cognitive decline of that individual by means of changed values of Mini-Mental State Examination (MMSE) over time. A MMSE value of 30 indicates a normal, cognitively healthy status. A MMSE value of less than 30, more preferably 25 or less, indicates MCI or dementia due to AD. The ratio of neurogranin on BACE1 in CSF may be used to assess or to monitor the rate of progression of Alzheimer's Disease, with a high ratio indicating a higher likelihood of a faster rate of decline than a lower ratio. An MCI or AD patient may be diagnosed by subjecting a CSF sample to the inventive method, wherein preferably a ratio of Neurogranin and BACE1 below 0.105 in that CSF sample) may indicate a decrease in MMSE of less than 0.42 MMSE units over the next 6 years. Preferably a ratio of Neurogranin and BACE1 between 0.105 and 0.144 may indicate a decrease in MMSE between 1.64 and 1.84 MMSE units over the next 6 years. Preferably a ratio of Neurogranin and BACE1 greater than 0.144 would indicate a decrease in MMSE more than 2.68 MMSE units over the next 6 years. In a preferred embodiment, the term “ratio of Neurogranin and BACE1”, as used herein, refers to the concentration in pg/mL of neurogranin in the sample of interest divided by the concentration in pg/mL of BACE1 in the same sample.
The present invention is further illustrated by the following non-limiting examples, sequences and figures from which further features, embodiments, aspects and advantages of the present invention may be taken.
Sequences in this application include
(a) epitope mapping of both mAbs AD×NGCI2 and AD×NGCT1, performed with overlapping synthetic peptides ranging from R51 to D78.
(b) specificity of the mAbs AD×NGCI2 and AD×NGCT1 was evaluated by Western blot analysis were both synthetic full-length neurogranin as well as a synthetic neurogranin peptide that is truncated at P75 were included in the gel electrophoresis, next to human collagen type I, III and IV. AD×NGCI2 positively stained both synthetic peptides, whereas AD×NGCT1 only labelled the truncated form. None of the mAbs detected the collagen proteins.
(c) analysis of several synthetic neurogranin peptides that differ in their C-terminus, i.e. intact (Peptide intact C-term) or either truncated at P75 (Peptide trunc P75) or at S76 (Peptide trunc S76), to confirm the specificity of the prototype ELISA towards neurogranin species truncated at P75 at different concentrations.
CSF samples were obtained by lumbar puncture (LP) at the L3/L4 or L4/L5 interspace, collected in polypropylene tubes, immediately frozen in liquid nitrogen and stored at −80° C. until analysis. Within 3 months prior to or after LP, neuropsychological examinations, at least consisting of Mini-Mental State Examination (MMSE) scores, were performed for each patient.
Patients with MCI due to AD (referred to as ‘MCI’ hereafter) (n=38) as well as age-matched patients with dementia due to AD (‘AD’) (n=50) were included and diagnosed according to the NIA-AA criteria (Albert et al. (2011) The diagnosis of mild cognitive impairment due to Alzheimer's disease: recommendations from the National Institute on Aging-Alzheimer's Association workgroups on diagnostic guidelines for Alzheimer's disease. Alzheimers Dement 7(3), 270-279; McKhann et al. (2011) The diagnosis of dementia due to Alzheimer's disease: recommendations from the National Institute on Aging-Alzheimer's Association workgroups on diagnostic guidelines for Alzheimer's disease. Alzheimers Dement 7(3), 263-269).
To discriminate AD from cognitively normal, CSF biomarkers Aβ1-42, T-tau and P-tau181P were already analyzed prior to this study at the BIODEM lab using commercial kits (Innotest β-AMYLOID(1-42), Innotest hTAU-Ag and Innotest PHOSPHO-TAU(181P); Fujirebio Europe, Ghent, Belgium), whereby the lab's cutoff values were applied [Van der Mussele et al. (2014) Depression in Mild Cognitive Impairment is associated with Progression to Alzheimer's Disease: A Longitudinal Study. J Alzheimers Dis 42(4), 1239-1250]. All MCI patients, of which 2 patients with a pathogenic AD gene mutation, had high probability of AD etiology according to the NIA-AA criteria (see also Supplementary Table 1). Regarding the participants of the age- and gender-matched cognitively healthy control group (‘CTRL’) (n=20), cognitive deterioration was ruled out by means of a neuropsychological screening. CTRL subjects had no neurological or psychiatric antecedents, central nervous system disorders or inflammatory syndromes.
If available, MMSE total scores from follow-up neuropsychological examinations (at least one year after LP (=baseline) were included in the study. This allowed calculating the rate of cognitive decline based on a linear mixed model adjusted for age and gender.
Ninety-six-well microtiter plates were coated with mAb AD×NGCI2 in Phosphate-buffered Saline (PBS), for 1 h at 21° C. After washing the wells with PBS containing Tween-20 (PBST), the plates were blocked with blocking reagent for 2 h at 21° C. The calibrator, a synthetic peptide containing the C-terminus of neurogranin truncated at Pro75 (custom-made by Proteogenix, France), was prepared in sample diluent (SD) (PBS based buffer with blocking reagent and detergents) at an initial concentration of 900 pg/mL and subsequent three-fold dilutions were made. Samples as well as calibrator dilutions were simultaneously incubated during 3 h at 21° C. with the biotinylated mAb AD×NGCT1 in SD. After washing the plates with PBST, horseradish peroxidase-conjugated streptavidin was added. After incubation of 30 min at 21° C., the plates were washed and a 3,3′,5,5′-tetramethylbenzidine solution was added to engage development. After 30 min at 21° C., the coloring reaction was stopped and plates were read in a BioTek microplate reader, at 450 nm. Levels of neurogranin trunc P75 were calculated via intrapolation (5PL curve fit; log(X)) based on the calibrator curve.
The BACE1 ELISA used is based on the ELISA method by Barão et al. ((2013) BACE1 levels correlate with phospho-tau levels in human cerebrospinal fluid. Curr Alzheimer Res 10(7), 671-678). This ELISA was further developed by implementing modifications allowing a simultaneous incubation (during 3 h) of 15 μL undiluted CSF sample and the detector antibody. Reagents from EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, Germany, were used (BACE-1 ELISA, product number EQ 6541-9601-L). Also, the inventors performed additional rounds of subcloning of the hybridoma cell line of one of the two involved antibodies to ensure the monoclonal character and production of the antibodies. This resulted in the mAb AD×402 (clone 10B8F1), which is the detector antibody in the ELISA, and which is biotinylated instead of conjugated with peroxidase as in the previous format (described by Barão et al., 2013). The hybridoma cell line of the capture antibody, mAb AD×401 (clone 5G7) underwent no additional rounds of subcloning. The inventors analyzed the BACE1 levels according to the new protocol, where concentrations were calculated via interpolation (5PL curve fit; log (X)) based on a calibrator curve.
1.4 ELISA for the CSF Biomarkers Total-Tau, AB1-42, AB1-40 and AB1-38 CSF levels of total-tau, Aβ1-42 and Aβ1-40 were re-analyzed during this study, using the total-tau ELISA, Beta-Amyloid (1-42) ELISA and Beta-Amyloid (1-40) ELISA kits commercially available from EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, Germany (Beta-Amyloid 1-38, product number EQ 6501-9601 for plasma, EQ 6501-9601-L for CSF; Beta-Amyloid 1-40 ELISA—EQ 6501-9601 for plasma, EQ 6501-9601-L for CSF, Beta-Amyloid 1-42 ELISA, product number EQ 6501-9601 for plasma, EQ 6501-9601-L for CSF).
Also, CSF Aβ1-38 was measured using the assay supplied by EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, Germany.
GraphPad Prism 6.02 and R version 3.1.2 were used for statistical analyses and figures. The strength of the correlation between analytes and/or parameters was expressed by Spearman's correlation coefficient. To compare quantitative variables data between groups, a Kruskal-Wallis test was applied. Linear mixed models were fitted with MMSE as dependent variable, fixed effects included time (continuous variable), CSF marker value and their interaction. The random effects included a random intercept for individual and a random slope for time. The significance of the interaction between the CSF marker value and time entailed whether the level of CSF marker had a significant effect on the MMSE change over time. The significance of this term was tested using an F-test with a Kenward-Roger correction for the number of degrees of freedom. Results were considered significant for P-values <0.05.
The anti-neurogranin mAbs AD×NGCI2 and AD×NGCT1 were generated by immunization of mice with synthetic peptides encompassing the C-terminus of neurogranin. The mAb AD×NGCI2 (isotype IgG2a) was produced at PharmAbs (K U Leuven, Belgium) in Balb/c mice after injections with a KLH-coupled peptide containing an internal sequence of C-terminal neurogranin (R51-D78) and additional boosts with full-length synthetic neurogranin. Both the peptide and full-size neurogranin were synthesized at Proteogenix (France). The mAb AD×NGCT1 (isotype IgG1) was isolated (at BIOTEM, France) from OF1 mice following an immunization with a KLH-conjugated peptide (synthesized at BIOTEM) corresponding to the C-terminus truncated at P75 (G60-P75). These two mAbs AD×NGCI2 and AD×NGCT1 each recognize a different epitope on neurogranin as depicted in
For measuring neurogranin, the inventors combined AD×NGCI2 as capture antibody and AD×NGCT1 as biotinylated detector antibody, into the research sandwich ELISA specific for neurogranin truncated at P75 (
In preparation of the clinical study, the inventors first verified the neurogranin trunc P75 assay as a proof-of-concept in a large set of CSF samples. Specifically, the inventors analyzed the control group for the clinical study (n=20) in addition to a cohort of diagnostically undefined CSF samples (n=161) (Biomnis, France). The inventors selected the latter group of samples within a wide range of age and with a similar number of male and female subjects. In total, 181 samples (104 female/77 male subjects) were analyzed with a median age of 50.3 (years) (range 7.0-92.1). Apart from neurogranin trunc P75, the inventors analyzed the CSF levels of BACE1, tau, Aβ1-42, Aβ1-40 and Aβ1-38. Table 1 summarizes the concentrations of all analytes. As depicted, only in a very minor number of samples neurogranin trunc P75 levels could not be determined, i.e. 2.8%, which was highly comparable to the other five analytes, i.e. 1.7% for total-tau, Aβ1-42 and Aβ1-40, 1.1% for Aβ1-38, and 0% for BACE1. In addition, all CSF analytes showed an association with age, albeit very weak in case of Aβ1-42: neurogranin trunc P75 (ρ=0.253; P=0.0007), BACE1 (ρ=0.412; P<0.0001), tau (ρ=0.366; P<0.0001), Aβ1-42 (ρ=0.147; P=0.050), Aβ1-40 (ρ=0.305; P<0.0001) and Aβ1-38 (p=0.326; P<0.0001).
After establishing the proof-of-concept of the research assay for neurogranin, the inventors investigated the levels of neurogranin trunc P75 and BACE1 in the CSF of MCI (n=38) and AD (n=50) patients versus cognitively normal participants (CTRL) (n=20). In addition, total-tau, Aβ1-42, Aβ1-40 and Aβ1-38 were quantified in the samples, which were tested blinded from clinical diagnosis. Table 3 sums up the levels of every analyte, combined with the demographic and clinical data of the population.
Compared to the CTRL group, CSF neurogranin trunc P75 was significantly increased in MCI patients (P<0.01), but was not significantly different in AD patients (Table 3,
Also, the inventors investigated the relationship between all CSF analytes in the clinical groups, and similar to the proof-of-concept analyses described above, there was a strong correlation between neurogranin trunc P75 and BACE1, in MCI (0.793, P<0.0001) as well as AD patients (0.711, P<0.0001) (Table 5). Moderately strong correlations between these synaptic analytes and total-tau were observed as well, i.e. neurogranin trunc P75 and tau were both correlated in MCI (0.635, P<0.0001), and AD (0.617, P<0.0001), as were BACE1 and tau in MCI (0.588, P<0.001), and AD (0.497, P<0.001). Even though neurogranin trunc P75, BACE1 and tau appeared closely associated, subtle differences became apparent when studying their respective associations with the Aβ-species. Neurogranin trunc P75 correlated with both Aβ1-40 and Aβ1-38 in MCI as well as AD, but in neither case with Aβ1-42. On the other hand, tau showed an albeit weak, yet significant correlation with Aβ1-42 in MCI (0.383, P<0.05), while BACE1 correlated with Aβ1-42 in AD (0.408, P<0.01). When considering the ratios of CSF Aβ1-42/Aβ1-40 and CSF Aβ1-42/Aβ1-38 more differences were found (Table 6). In the MCI population, both neurogranin trunc P75 and BACE1 were correlated with both ratios, whereas tau was not.
1.9 Relationship of CSF Biomarkers with MMSE Decline
In addition to their diagnostic performance, the inventors evaluated the analytes as possible progression biomarkers by assessing their relationship with cognitive decline. Therefore, the inventors considered the CSF levels of all analytes, as single parameters as well as ratios, in a subset of MCI (n=36) and AD (n=45) patients where MMSE was monitored. Adjusted for age and gender, a linear mixed model analysis, with the longitudinal MMSE measurements as outcome, demonstrated that none of the single analytes at baseline, i.e. neurogranin trunc P75, BACE1 nor the classic CSF AD biomarkers, were associated with the rate of cognitive decline (Table 7). Also the majority of biomarker ratios was not correlated, although, there was one specific ratio of CSF biomarkers showing a consistently significant impact on the longitudinal decline in MMSE in both MCI and AD patients, i.e. CSF neurogranin trunc P75/BACE1 (β=−0.018; P<0.05 for MCI; β=−0.051; P<0.01 for AD). Statistical significance remained unchanged in case two possible confounders, CSF levels of tau and Aβ1-42, were added to the model as fixed effects. To illustrate this correlation between the neurogranin trunc P75/BACE1 ratio and cognitive deterioration, the subset of patients with MMSE at and follow-up after LP was partitioned into three tertiles according to the ratio levels (
Combining AD×NGCI2 as capture antibody and AD×NGCT1 as biotinylated detector antibody resulted in the research sandwich ELISA specific for neurogranin truncated at P75 (
In preparation of the clinical study, the inventors first verified the neurogranin trunc P75 assay as a proof-of-concept in a large set of CSF samples. Specifically, the control group for the clinical study (n=20) in addition to a cohort of diagnostically undefined CSF samples (n=161) (Biomnis, France) was analyzed. The latter group of samples within a wide range of age and with a similar number of male and female subjects was selected. In total, 181 samples (104 female/77 male subjects) were analyzed with a median age of 50.3 (years) (range 7.0-92.1). Apart from neurogranin trunc P75, we analyzed the CSF levels of BACE1, tau, Aβ1-42, Aβ1-40 and Aβ1-38. Table 1 summarizes the concentrations of all analytes. As depicted, only in a very minor number of samples neurogranin trunc P75 levels could not be determined, i.e. 2.8%, which was highly comparable to the other five analytes, i.e. 1.7% for total-tau, Aβ1-42 and Aβ1-40, 1.1% for Aβ1-38, and 0% for BACE1. In addition, all CSF analytes showed an association with age, albeit very weak in case of Aβ1-42: neurogranin trunc P75 (ρ=0.253; P=0.0007), BACE1 (ρ=0.412; P<0.0001), tau (ρ=0.366; P<0.0001), Aβ1-42 (ρ=0.147; P=0.050), Aβ1-40 (ρ=0.305; P<0.0001) and Aβ1-38 (ρ=0.326; P<0.0001).
The inventors investigated the levels of neurogranin trunc P75 and BACE1 in the CSF of MCI (n=38) and AD (n=50) patients versus cognitively normal participants (CTRL) (n=20). In addition, total-tau, Aβ1-42, Aβ1-40 and Aβ1-38 were quantified in the samples, which were tested blinded from clinical diagnosis. Table 3 sums up the levels of every analyte, combined with the demographic and clinical data of the population.
Compared to the CTRL group, CSF neurogranin trunc P75 was significantly increased in MCI patients (P<0.01), but was not significantly different in AD patients (Table 3,
Also, the inventors investigated the relationship between all CSF analytes in the clinical groups, and similar to the proof-of-concept analyses described above, there was a strong correlation between neurogranin trunc P75 and BACE1, in MCI (0.793, P<0.0001) as well as AD patients (0.711, P<0.0001) (Table 5). Moderately strong correlations between these synaptic analytes and total-tau were observed as well, i.e. neurogranin trunc P75 and tau were both correlated in MCI (0.635, P<0.0001), and AD (0.617, P<0.0001), as were BACE1 and tau in MCI (0.588, P<0.001), and AD (0.497, P<0.001). Even though neurogranin trunc P75, BACE1 and tau appeared closely associated, subtle differences became apparent when studying their respective associations with the Aβ-species. Neurogranin trunc P75 correlated with both Aβ1-40 and Aβ1-38 in MCI as well as AD, but in neither case with Aβ1-42. On the other hand, tau showed an albeit weak, yet significant correlation with Aβ1-42 in MCI (0.383, P<0.05), while BACE1 correlated with Aβ1-42 in AD (0.408, P<0.01). When considering the ratios of CSF Aβ1-42/Aβ1-40 and CSF Aβ1-42/Aβ1-38 more differences were found (Table 6). In the MCI population, both neurogranin trunc P75 and BACE1 were correlated with both ratios, whereas tau was not.
2.4 Relationship of CSF Biomarkers with MMSE Decline
The inventors considered the CSF levels of all analytes, as single parameters as well as ratios, in a subset of MCI (n=36) and AD (n=41) patients where MMSE was monitored longitudinally post-LP, for at least one year. Adjusted for age and gender, a linear mixed model analysis, with the longitudinal MMSE measurements as outcome, demonstrated that none of the single analytes at baseline, i.e. neurogranin trunc P75, BACE1 nor the classic CSF AD biomarkers, were associated with the rate of cognitive decline. However, there was one specific ratio of CSF biomarkers showing a consistently significant impact on the longitudinal decline in MMSE across both the MCI and AD group, i.e. CSF neurogranin trunc P75/BACE1 (β=−0.018; P<0.05 for MCI; β=−0.051; P<0.01 for AD) (
In the sample set of the current study, the inventors have shown that the ratio of CSF Neurogranin trunc P75/BACE1 correlates with ongoing and future cognitive decline in MCI due to AD as well as dementia due to AD, in contrast to neurogranin trunc P75 and BACE1 as single parameter, or in contrast to the current AD CSF biomarkers total-tau, Aβ42 or Aβ40.
0.003
<0.0001
<0.0001
0.001
<0.0001
0.038
<0.0001
<0.0001
<0.0001
<0.0001
<0.0001
<0.0001
<0.0001
<0.0001
0.008
<0.0001
0.023
0.036
0.005
| Number | Date | Country | Kind |
|---|---|---|---|
| 16001006.2 | May 2016 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2017/000549 | 5/3/2017 | WO | 00 |
