Patent Application
20210080459
Toxoplasma gondii (T. gondii) is an obligate intracellular parasite with a worldwide distribution capable of infecting virtually any warm-blooded animal including humans.
This parasite has been considered as one of the most successful eukaryotic pathogens concerning the number of host species and percentage of animals infected globally. It has been estimated that approximately 1 billion people worldwide are infected by T. gondii. Most infections are asymptomatic or take the form of a mild, self-limiting illness characterized by fever, malaise and lymphadenopathy. Nevertheless, in situations of immunodeficiency or when the parasite is congenitally acquired, T. gondii can cause severe disease and even death if not treated properly. Generally, there are 3 main complications associated with toxoplasmosis including ocular toxoplasmosis, congenital toxoplasmosis, and cerebral toxoplasmosis. Moreover, recent epidemiological data shed light on new pathogenic potential of the parasite. For example, highly virulent strains originated from Amazon rainforest can cause severe disease and even death in immunocompetent individuals. The tropism of T. gondii for the brain has also been linked to some mental disorders including schizophrenia, personality changes, dementia and suicidal tendencies. For both immunocompetent and immunocompromised individuals, there is considerable variation of T. gondii infection in terms of the clinical presentation and severity of disease. For example, not all AIDS patients with positive serology for T. gondii develop toxoplasma encephalitis (TE). TE has been estimated in about 20-30% of seropositive AIDS patients. Similarly, women who acquire infection with T. gondii during pregnancy do not always lead to congenital infections in the newborn. About one-third of women who seroconvert to T. gondii during pregnancy transmit the parasite to the fetus. Also, the presentation of congenital toxoplasmosis varies widely from subclinical to severe cases, which may cause fetal or neonatal death. In immunocompetent individuals, while most infections result in no detectable symptoms, a small percentage of infections develop a severe form of disease as evidenced by the prevalence of ocular toxoplasmosis or mental disorders. The mechanisms by which infections either become mild or severe disease are not clear. Given the variable and sometimes severe consequences of T. gondii infection, it is of clinical and epidemiological importance to identify the factors that contribute to these different outcomes.
The present invention identified factors that will help to identify high-risk populations or individuals, to diagnose disease conditions promptly and accurately, and to effectively treat patients with disease. In particular, the present invention will allow us to: i) identify patients at high risk of developing a severe disease, especially when they exhibit an immunocompromised status; ii) identify mothers of infants with congenital toxoplasmosis and an informed decision can be made earlier in terms of treatment or abortion; iii) treat infected at-risk infants early, whether or not they have signs of the infection, may reduce the incidence of sequelae.
A first embodiment of the present invention is a method of identify one or more health risks in immunocompromised patients with positive serology for T. gondii, such as those with AIDS, neoplastic diseases and organ transplants. This includes the following steps: obtaining a biological sample from a subject; determining the level of T. gondii cyst antigen antibody in the biological sample; and characterizing the biological sample in at least one of the three categories: (i) high T. gondii cyst antigen antibody level; (ii) low T. gondii cyst antigen antibody level; or (iii) substantially free T. gondii cyst antigen antibody level. A suitable T. gondii cyst antigen is a MAG1 antigen, preferably MAG1_4, MAG1_5, or a combination thereof. A suitable reporting method of determining the level of T. gondii cyst antigen antibody within a biological sample taken from a subject is ELISA. After testing each biological sample for T. gondii cyst antigen antibody levels the biological samples were characterized into one of the three categories: (i) a high T. gondii cyst antigen antibody level of greater than 0.5 OD preferably in the range from 0.55, 0.60, 0.65, 0.70, 0.75, 0.80, 0.85 to 0.90, 0.95; (ii) a low T. gondii cyst antigen antibody level in the range of 0.06 to 0.49 OD, preferably 0.06, 0.07, 0.8 to 0.35, 0.40, 0.45, 0.49; (iii) a substantially free T. gondii cyst antigen antibody level in the range of 0 to 0.059 OD. Should a biological sample from a subject have a high T. gondii cyst antigen antibody level it is preferred to have an early intervention to prevent high risk of developing a severe disease.
A second embodiment of the present invention is a method of identify one or more health risks in a woman who acquire infection with T. gondii during pregnancy comprising the following steps: obtaining a biological sample from a subject; determining the levels of T. gondii cyst antigen antibody in the biological sample; and characterizing the biological sample in at least one of three categories (i) a high T. gondii cyst antigen antibody level; (ii) a low T. gondii cyst antigen antibody level; or (iii) a substantially free T. gondii cyst antigen antibody level. A suitable T. gondii cyst antigen is a MAG antigen, preferably MAG1_4, MAG1_5, or a combination thereof. Suitable reporting methods of determining the level of T. gondii cyst antigen and T. gondii organism antibody levels includes ELISA. After testing each biological sample for T. gondii cyst antigen antibody levels the biological samples were characterized into one of the three categories: (i) a high T. gondii cyst antigen antibody level of greater than 0.5 OD preferably in the range from 0.55, 0.60, 0.65, 0.70, 0.75, 0.80, 0.85 to 0.90, 0.95; (ii) a low T. gondii cyst antigen antibody level in the range of 0.06 to 0.49 OD, preferably 0.06, 0.07, 0.8 to 0.35, 0.40, 0.45, 0.49; and (iii) a substantially free T. gondii cyst antigen antibody level in the range of 0 to 0.059 OD. A substantially free T. gondii organism antibody level has an OD in the range of 0 to 0.049. A biological sample having a T. gondii organism antibody level preferably has an OD greater than 0.050. Should a biological sample from a subject have a high T. gondii cyst antigen antibody level be identified then it is preferred to discuss the results with the subject including an earlier decision in terms of treatment such as additional T. gondii drug therapy or abortion if a pregnant woman is infected.
A third embodiment of the present invention is a method of identify one or more health risks in a child with congenital T. gondii infection comprising the following steps: obtaining a biological sample from a subject; determining the level of T. gondii cyst antigen antibody in the biological sample; and characterizing the biological sample in at least one of the three categories: (i) high T. gondii cyst antigen antibody level; (ii) low T. gondii cyst antigen antibody level; or (iii) substantially free T. gondii cyst antigen antibody level. A suitable T. gondii cyst antigen is a MAG1 antigen, preferably MAG1_4, MAG1_5, or a combination thereof. A suitable reporting method of determining the level of T. gondii cyst antigen antibody within a biological sample taken from a subject is ELISA. After testing each biological sample for T. gondii cyst antigen antibody levels the biological samples were characterized into one of the three categories: (i) a high T. gondii cyst antigen antibody level of greater than 0.5 OD preferably in the range from 0.55, 0.60, 0.65, 0.70, 0.75, 0.80, 0.85 to 0.90, 0.95; (ii) a low T. gondii cyst antigen antibody level in the range of 0.06 to 0.49 OD, preferably 0.06, 0.07, 0.8 to 0.35, 0.40, 0.45, 0.49; (iii) a substantially free T. gondii cyst antigen antibody level in the range of 0 to 0.059 OD. Should a biological sample from a subject have a high T. gondii cyst antigen antibody level it is preferred to start treatment to reduce the incidence of sequelae.
A fourth embodiment of the present invention is a method of identify one or more health risks in immunocompetent individuals with positive serology for T. gondii comprising the following steps: obtaining a biological sample from a subject; determining the level of T. gondii cyst antigen antibody in the biological sample; and characterizing the biological sample in at least one of the three categories: (i) high T. gondii cyst antigen antibody level; (ii) low T. gondii cyst antigen antibody level; or (iii) substantially free T. gondii cyst antigen antibody level. A suitable T. gondii cyst antigen is a MAG antigen, preferably MAG1_4, MAG1_5, or a combination thereof. A suitable reporting method of determining the level of T. gondii cyst antigen antibody within a biological sample taken from a subject is ELISA. After testing each biological sample for T. gondii cyst antigen antibody levels the biological samples were characterized into one of the three categories: (i) a high T. gondii cyst antigen antibody level of greater than 0.5 OD preferably in the range from 0.55, 0.60, 0.65, 0.70, 0.75, 0.80, 0.85 to 0.90, 0.95; (ii) a low T. gondii cyst antigen antibody level in the range of 0.06 to 0.49 OD, preferably 0.06, 0.07, 0.8 to 0.35, 0.40, 0.45, 0.49; (iii) a substantially free T. gondii cyst antigen antibody level in the range of 0 to 0.059 OD. Should a biological sample from a subject have a high T. gondii cyst antigen antibody level it is preferred to be monitored or treated to avoid the incidence of ocular toxoplasmosis or mental disorder.
As used herein, the term “high-risk” refers to an enhanced risk of having one or more health problems such as birth defects, sequelae, ocular or mental disorder, ocular toxoplasmosis, congenital toxoplasmosis, and cerebral toxoplasmosis, for example. The term “risk” refers to the propensity of a subject to have one or more health problems.
As used herein, the term “T. gondii cyst antigen antibody” refers to the antibody that is capable of binding to the T. gondii MAG1 antigen, or fragments thereof, preferably MAG1_4 or MAG1_5 as examples. Examples of cyst antigen protein sequences include NCBI Accession Number AAC46484, NCBI Accession Number EPT28403.1, and NCNI Accession Number KYF46731.1.
As used herein, the term “subject” refers to any individual or patient to which the method described herein is performed. Generally the subject is human, although as will be appreciated by those in the art, the subject may be an animal. Thus other animals, including mammals such as rodents (including mice, rats, hamsters and guinea pigs), cats, dogs, rabbits, farm animals including cows, horses, goats, sheep, pigs, etc., and primates (including monkeys, chimpanzees, orangutans and gorillas) are included within the definition of subject.
As used herein, the term “treatment” refers to minimizing the impact of a health related problem through drug therapy, surgery, psychiatric assistance, for example.
The intracellular protozoan Toxoplasma gondii is an exceptionally successful parasite that infects approximately 1 billion people worldwide. Latent infection persists during the lifespan of the intermediate hosts such as human through the formation of cysts in muscle and brain. Although genotyping of T. gondii isolates from all continents reveals a complex population structure, the majority of strains isolated in North America and Europe fall into one of three clonal lineages: types I, II and III. Among the three clonal lineages, type I strains are lethal in mice, but type II and III strains are considerably less virulent. Pathogenicity differences among the three strains are largely determined by genetic polymorphisms and differences in expression level of secretory proteins released from dense granule and rhoptry organelles (e.g. GRA15, ROP5, ROP16 and ROP18).
Human infections with T. gondii display a wide range of clinical symptoms. This variation is likely to be a consequence of many factors including human and parasite genotypes, timing of infection, and environmental factors such as co-infections and nutrition. Regarding parasite genotypes, virulent strains are found to be associated with increased frequency and severity of human toxoplasmosis. For example, several studies have suggested that type I strains are more pathogenic in immunocompromised patients. Khan et al. analyzed 11 cerebral spinal fluid (CSF) samples collected from patients who had confirmed or presumptive toxoplasmosis encephalitis. They found a majority of these patients had infections with type I strains or strains containing type I alleles. Ferreira et al. investigated the genotypes of T. gondii strains isolated from 87 patients with cerebral toxoplasmosis and AIDS, treated in Sao Paulo State, Brazil. Although their study revealed a high rate of genetic polymorphism in T. gondii strains, type I seems to be most prevalent as this strain was responsible for infection in 46% of their patients. In a small series of patients with severe ocular inflammation from the United States, there was an unusual abundance of type I or atypical parasites. Evidence from serotyping indicated that the offspring of mothers with T. gondii type I infection were at significantly increased risk for the development of psychoses as compared with the matched unaffected control mothers. In the case of congenital toxoplasmosis, McLeod et al. determined the parasite serotype for 193 congenitally infected infants and their mothers in the NCCCTS, 1981-2009. The authors noted that NE-II (all non-type II) serotypes are more often present than type II serotypes in infants with congenital toxoplasmosis (61% vs. 39%) in the United States. Moreover, infants with NE-II serotypes are more likely to experience severe disease at birth than those with type II serotypes. Several hypotheses such as strain-related differences in pathogenicity, poor host (human) adaption and human genetic predisposition have been suggested to account for the strain-specific pathology.
Type I strains of T. gondii are excellent in vitro models, but most strains of this type do not readily develop tissue cysts or latent infection in laboratory mice. In contrast, type II strains easily establish latent infections in mice characterized by the presence of tissue cysts, a fact that could explain their common usage for chronic studies. Given the association of type I strains with severity of human toxoplasmosis, we sought to investigate its chronic effect using a murine model. The effect of type I strain infection was assessed by changes on behavior, cytokine and gene expression. Previously, we have developed an assay which measures humoral immune response against T. gondii matrix antigen MAG1 using synthetic peptides. Employing samples from patients with and without clinical toxoplasmosis, we have shown that the MAG1 antibody level can differentiate active from inactive human toxoplasmosis. We thus hypothesized that behavioral change or the presence of pathology in chronically infected mice may be associated with high levels of MAG1 antibody.
Kinetics of Antibody Response to T. gondii MAG1 Antigen
Because GT1 is a virulent strain, we administered anti-T. gondii chemotherapy to ensure mouse survival. To investigate the kinetics of antibody response, a pilot study (n=10) with sera collected weekly from 2 to 18 weeks after infection was examined. We measured antibody response to both T. gondii organism and MAG1 peptides (MAG1_4 and MAG1_5). As seen in
Since our pilot study suggested that GT1-exposed mice displayed variability in the MAG1 antibody generation, we first characterized antibody profiles in mice used for behavioral assessment. After the last behavioral test, sera were collected and measured for their antibody levels to T. gondii and MAG1 antigen. For T. gondii IgG antibody, 72% of the GT1-exposed mice (33 out of 46) were seropositive and there was little inter-animal variation in the antibody levels (IgG=3.72+0.09, mean+SEM). For MAG1 antibody, the cut-off value for a positive response (OD=0.07) was defined as mean plus 4 standard deviation of the control samples. Using this value, MAG1 antibody was detected in 46% of GT1-exposed mice (21 out of 46) and their levels varied greatly (MAG1=1.23+0.30). Therefore, mice were stratified into high MAG1 level (MAG1=2.38+0.36, n=10) and low MAG1 level (MAG1=0.19+0.04, n=11) groups based on the distribution of MAG1 antibody levels. According to antibody profiles, we categorized the mice into 5 groups, as follows: (i) unexposed control (contain neither of these antibodies, n=18); (ii) mice with high MAG1 antibody level (IgG+/MAG+high, MAG1 antibody level>0.5, n=10); (iii) mice with low MAG1 antibody level (IgG+/MAG+low, MAG1 antibody level<0.5, n=11); (iv) mice without MAG1 antibody (IgG+/MAG1−, n=12); (v) mice exposed to T. gondii that did not develop any antibody response (IgG−/MAG1−, n=13).
Because MAG1 antigen is abundantly expressed in the cyst matrix, we next investigated the relationship between MAG1 antibody level and the number of tissue cysts in the brain (Table 1). In a separate cohort of mice 20 wpi, we measured the number of tissue cysts in half of the brain in T. gondii-seropositive mice (IgG+, n=16). Tissue cysts of T. gondii were found in brains of all MAG1-seropositive mice (MAG1+, n=11), while no cyst was detected in MAG1-seronegative mice (MAG1−, n=5). Using Spearman Rank analysis, significant positive correlation between MAG1 antibody level and the number of brain cysts was found (r=0.82, p=0.0021, Table 1). Moreover, there was also a significant correlation between MAG1 antibody level and the average size of tissue cysts within the brain (r=0.89, p=0.0003, Table 1). If mice were categorized into the predefined MAG1+high or MAG1+low group, there was a significant difference in cyst burden between the groups (median cyst burden for MAG1+high, 175.5, and MAG1+low, 52.0, p=0.0043, Mann-Whitney test)
Furthermore, the relationship between MAG1 antibody level and expression of bradyzoite-specific gene (BAG1) was investigated in the prefrontal cortex of mouse. The expression of BAG1 gene was detected by qPCR and sample was considered positive after reaching the threshold (<40 cycles). Our results showed that the positive rate of BAG expression was associated with MAG1 antibody level (p=0.011). In mice with high MAG1 levels, the positivity rate was 87.5% (7 out of 8). In contrast, the positivity rate was lower in mice with low MAG1 levels (25%, 2 out of 8). No BAG1 gene expression was detected in unexposed control mice, exposed mice without IgG antibody (IgG−/MAG1−), or mice without MAG1 antibody (IgG+/MAG1−). To identify whether there is bradyzoite-to-tachyzoite interconversion, we measured the expression of tachyzoite specific gene SAG1 among different groups of mice. Our results demonstrated that SAG expression was undetectable in samples from all the GT1-exposed mice (data not shown).
aSpearman's correlation coefficient (r) and p value between MAG1 antibody level and the number of brain tissue cysts.
bSpearman's correlation coefficient (r) and p value between MAG1 antibody level and the average size of brain tissue cysts.
Poor Body Weight Gain in Mice with High MAG1 Level
To examine the effect of chronic T. gondii GT1 infection on body weight, mice were weighed weekly and weights were analyzed by repeated measures ANOVA with group as a between-subject factor and time as a within-subjects factor. The results revealed a significant effect for time, F (4, 59)=51.303, p<0.0005, and a significant interaction between time and group, F (4, 59)=1.642, p=0.004 (Wilks' Lambda). As seen in
We observed a negative correlation (r=−0.46, p=0.0365,
Behavioral Deficits in Mice with High MAG1 Level
Behavioral testing was conducted between 12 to 21 weeks following infection. In summary, mice with high MAG1 antibody level exhibited reduced locomotor and exploratory activity, impaired object recognition memory when tested at a 24 h delay, and lack of response to amphetamine induced activity. No significant group differences occurred in the Y-maze test which was used to evaluate spatial working and recognition memory (Ps>0.5).
As a commonly used model to assess novelty-induced activity, we conducted open field test to evaluate general locomotor and exploratory activity in T. gondii GT1-infected mice. As depicted in
The effect of T. gondii infection on recognition memory of mice was assessed by novel object recognition, which utilizes the innate tendency of rodents to preferentially explore novel objects. A preference score of 50% indicates random exploration, while scores higher than chance reflect intact memory. As seen in
The role of dopamine has been suspected in T. gondii-induced behavioral alterations. As a dopamine stimulant, we measured mouse response to amphetamine-trigged locomotor activity in the open field. As depicted in
To examine inflammatory response to chronic T. gondii GT1 infection, we assessed 23 cytokine levels in the prefrontal cortex of mice from different groups. In addition, we examined relationships between these cytokine responses and behavior changes observed, with the intent to investigate whether inflammation related to these behavior responses.
Among the 23 cytokines, five differed significantly between controls and mice with high MAG1 antibody level (Table 2). In mice with high MAG1 levels, IL-1α (p=0.003), IL-12p70 (p=0.004), and TNF-α (p=0.013) levels were decreased, while CCL5 (p=0.008) and subunit IL-12p40 (p=0.013) levels were elevated. We found that all altered cytokines except IL-1α displayed correlations with MAG1 antibody level. As depicted in
aIgG+/MAG1 + high: mice with high MAG1 antibody level;
bIgG+/MAG1 + low: mice with low antibody MAG1 level;
cIgG+/MAG1-: mice without MAG1 antibody;
dIgG−/MAG1-: mice exposed to T. gondii that did not develop any antibody response.
ep value between IgG+/MAG1 + high and unexposed control (ANOVA followed by Bonferroni's post hoc). N = 8 per group.
We then investigated whether these cytokine levels are related to behavioral changes. Logistic regressions, with or without adjusting for serological status (T. gondii and MAG1 antibody), were performed for all of the cytokines in relation to any of the 4 behaviors (novel activity, rearing, novel object recognition, and amphetamine-induced activity). The results of the unadjusted and adjusted cytokine analyses are shown in Table 3. In the adjusted analyses, we found that all cytokines except IL-10 were no longer significantly associated with behavioral responses. The adjusted p value for IL-10 was 0.042.
0.042
b
aThe levels of cytokines were tested for association with behavior using a logistic regression model employing T. gondii and MAG1 antibody groups as covariates. Shown are regressions with R2 values above 0.10 (which corresponds to a p value ≤0.05) for all of the cytokines correlated with any of the 4 behaviors tested (novel activity, rearing, novel object recognition, and amphetamine-induced activity). After adjusting for serological status, all cytokines except IL-β were no longer associated with behavior responses. The significant adjusted p value is indicated in bold.
Because changes in neurotransmitter concentration would affect behavioral performance, we measured biogenic amines and their metabolites in the mouse striatum. There were no significant differences (p>0.05) in neurotransmitter (dopamine; serotonin) or metabolite (3,4-dihydroxyphenylacetic acid; homovanillic acid; 3-methoxy-4-hydroxyphenylglycol; and 5-hydroxyindoleacetic acid) concentrations among the five groups (data not shown).
Gene Expression of miR-132
Because miR-132 alteration has been characterized in mouse models infected by T. gondii and its dysregulation has important implications for behavioral changes, we evaluated striatal miR-132 expression among different groups of mice. The selection of striatum is based on previous finding where significant change of miR-132 was noticed. Employing qPCR analysis, we found a decrease in the expression of miR-132 in mice with high MAG1 level (F(4,28)=6.403, p=0.0009; Ps<0.05 between IgG+/MAG1+high vs all other groups,
To provide insights into virulent strain-related severity of human toxoplasmosis, we established a chronic model of the virulent type I strain using outbred mice. We found that type I-exposed mice displayed variable outcomes ranging from aborted to severe infections, characterized by antibody profiles, pathology and behavior changes. The mechanisms by which infections either resolve in the acute phase or become chronic are not clear. However, differences in host immune response or sensitivity to anti-T. gondii chemotherapy might be involved. According to antibody profiles, we found that most of mice generated antibodies against T. gondii organism but varied greatly in the MAG1 antibody development. There was a strong correlation between MAG1 antibody level and brain cyst burden. We found that mice with high MAG1 antibody level displayed weight loss, behavioral changes, altered levels of gene expression and immune activation. The extent of most changes was directly correlated with levels of MAG1 antibody. These changes were not found in mice with less cyst burden or mice that were acutely but not chronically infected. Our finding highlights the critical role of cyst burden in a range of disease severity during chronic infection, the predictive value of MAG1 antibody level to brain cyst burden and to changes in behavior or other pathology in chronically infected mice.
In the present study, mouse response to infection was characterized by serum antibody levels to T. gondii organism and MAG1 antigen. Although it has been shown that MAG1 antigen is expressed during both tachyzoite and bradyzoite development, we found MAG1 antibody level was highly associated with cyst burden within the chronically infected brain, determined by the number of tissue cysts, by the average size of cysts, and by the bradyzoite prevalence in the mouse prefrontal cortex. Presumably, the association is due to the fact that MAG1 is abundantly expressed within the cyst and in the cyst wall surrounding the bradyzoites. These results suggest that MAG1 antibody level is a parameter with predictive value with regard to cyst burden in infected mice.
We previously reported that MAG1 antibody level can distinguish active from inactive human toxoplasmosis. Similarly, in the current study, the degree of symptomatology of brain pathology and behavioral changes in chronically infected mice was found to be associated with MAG1 antibody level. In contrast, many of these changes were not found in mice with low MAG1 antibody level or mice that lack of MAG1 antibody. Given MAG1 antibody level and brain cyst burden were highly correlated, this finding is in agreement with recent human studies reporting that the severity of toxoplasmosis in patients is associated with parasite burden. For instance, Stajner et al. reported a fatal T. gondii reactivation in a human stem cell transplant recipient with underlying immunological deficiency, who had extremely high parasite burden in blood and BAL fluid. In the case of favorable outcome of reactivation in a heart transplant patient, a much low parasite load was observed.
Previously, the dopamine antagonists haloperidol and GBR 12909 were found to prevent the behavioral alterations in T. gondii-infected rats, suggesting dopamine has a role in T. gondii-induced behavioral changes. In the present study, we found that mice with high MAG1 antibody level displayed a striking behavioral deficit in amphetamine-trigged locomotor response. Our results are in agreement and extend prior studies by suggesting chronic T. gondii infection can trigger abnormal response to dopamine stimulant (amphetamine). Interestingly, this deficit was found not only associated with MAG1 antibody level, but also associated with IL-10 level. Amphetamine is thought to exert its stimulant effect by elevating synaptic concentrations of dopamine in brain reward areas. However, this deficit does not seem to be due to changes in striatal levels of dopamine, serotonin, and their metabolites, since no change was found in the striatum of mice with high MAG1 antibody level during chronic infection. This result is consistent with several reports describing no changes in monoamines and their metabolites during chronic infection. In order to elucidate the mechanisms behind this deficit, dopamine transmission-related pathways might be considered in future studies.
Cognitive deficit has been reported in mice after infection with T. gondii. In agreement with previous finding, there seems to be a general cognitive deficit in recognition memory in all T. gondii IgG seropositive mice (groups of MAG1 high, MAG1 low and without MAG1), although the differences did not reach significance in mice with MAG1 low and without MAG1 groups. Moreover, there was no correlation between cognitive impairment and MAG1 antibody levels. Our results suggested that immune response, not the presence of brain cysts, might contribute to the cognitive deficit. Although no correlation was found between cytokines and this deficit, this may depend on the cytokines involved. Interestingly, cognitive deficit was found at long time retention (24 h), but not at short time retention (1 h). This finding is in agreement with observation made by Gulinello et al., who reported normal cognitive function after a 45 min delay in the object recognition assay.
Another possible reason for cognitive deficit in T. gondii IgG seropositive mice might include miR-132 dysregulation during infection. Similar to our previous result, we found miR-132 was downregulated during chronic infection in mice with high MAG1 antibody level. However, miR-132 was found to be upregulated during acute infection regardless of the parasite genotype, a phenomenon related to its effects on infection and inflammation. It is worth noting that transgenic mice overexpressing miR-132 exhibited increased neuronal spine density but impaired novel object recognition memory. Given miR-132 was upregulated before entering the chronic phase, this might be a potential cause for the observed deficit. Lately, miR-132 has been demonstrated to affect multiple neuronal functions and its dysregulation is linked to several neurological disorders. Thus, miR-132 dysregulation provides a possible mechanism for changes in behavior and warrant further investigation.
In mice with high MAG1 antibody level, the expression of 3 proinflammatory cytokines (IL-1α, IL-12p70, and TNF-α) was decreased and expression of IL-12p40 and CCL5 was increased. The suppression of 3 proinflammatory cytokines indicated that the host did not mount a strong response directed at alerting and activating the immune system to react to the infection. These observations are in agreement with findings in humans. Yamamoto et al. observed that asymptomatic persons had higher levels of IL-12 in response to T. gondii antigens than patients with ocular lesions. De-la-Torre et al. reported that Colombian patients with severe ocular toxoplasmosis displayed a suppressive immune response, although the specific cytokines differ from our study. However, ongoing brain inflammation seems to be suggested, as evidenced by increased expression of markers associated with protective immunity. For example, as a well-established chemoattractant for T cells, the increased level of CCL5 could represent a potential mechanism that mediates CNS inflammation.
Here we reported that several behavioral abnormalities occurred in the mice with high MAG1 antibody level. Given this group of mice exhibited lower weight, this might be a confounding factor that contributes to the differences in behavior. It is possible that decreased activity in the open field may be due to lower weight, as sick mice do not move as much. However, the deficit seen in novel object recognition is less likely due to lower weight considering the deficit wasn't observed at 1 hr retention, but was seen at 24 hrs retention (presumably the weights were still lower). Similarly, the failed response to amphetamine could not simply be ascribed to the mice being lower weight, because it looks like all groups decreased their activity after the saline administration and are about the same before amphetamine treatment. Certainly, more experiments are necessary to confirm that this difference in response is specific to the amphetamine or dopamine pathway.
In conclusion, we were able to establish a model of chronic infection with virulent type 1 strain and to determine correlates of disease pathogenesis. Although it is known that parasite burden is associated with the degree of symptomatology in human toxoplasmosis, the present results have three major findings. First, MAG1 antibody level has predictive value for brain cyst burden and for changes in behavior or other pathology in chronically infected mice. Second, chronic T. gondii infection could trigger abnormal response to dopamine stimulant (amphetamine). Finally, our study suggested that some behaviors are associated with T. gondii itself (latent infection), whereas other behaviors do not require the presence of brain cysts (may be related to acute infection). This model may be useful in the performance of translational studies relating to human toxoplasmosis. These variable phenotypes displayed in this mouse model may reflect the diversity of human responses to T. gondii and lead to an increased understanding of mechanisms underlying pathogenic changes following infection.
Any of the compositions described herein may be comprised in a kit. In a non-limiting example, a MAG1 antibody and/or a T. gondii antibody may be comprised in a kit.
The kits may comprise a suitably aliquoted of a MAG1 antibody and/or a T. gondii antibody and, in some cases, one or more additional agents. The component(s) of the kits may be packaged either in aqueous media or in lyophilized form. The container means of the kits will generally include at least one vial, test tube, flask, bottle, syringe or other container means, into which a component may be placed, and preferably, suitably aliquoted. Where there are more than one component in the kit, the kit also will generally contain a second, third or other additional container into which the additional components may be separately placed. However, various combinations of components may be comprised in a vial. The kits of the present invention also will typically include a means for containing a MAG1 antibody and/or a T. gondii antibody and any other reagent containers in close confinement for commercial sale. Such containers may include injection or blow-molded plastic containers into which the desired vials are retained.
When the components of the kit are provided in one and/or more liquid solutions, the liquid solution is an aqueous solution, with a sterile aqueous solution being particularly preferred. A MAG1 antibody and/or a T. gondii antibody may be formulated into a syringeable composition. In which case, the container means may itself be a syringe, pipette, and/or other such like apparatus, from which the formulation may be applied to an infected area of the body, injected into an animal, and/or even applied to and/or mixed with the other components of the kit.
However, the components of the kit may be provided as dried powder(s). When reagents and/or components are provided as a dry powder, the powder can be reconstituted by the addition of a suitable solvent. It is envisioned that the solvent may also be provided in another container means.
The following Examples have been included to provide guidance to one of ordinary skill in the art for practicing representative embodiments of the presently disclosed subject matter. In light of the present disclosure and the general level of skill in the art, those of skill can appreciate that the following Examples are intended to be exemplary only and that numerous changes, modifications, and alterations can be employed without departing from the scope of the presently disclosed subject matter. The following Examples are offered by way of illustration and not by way of limitation.
Mouse Model of Chronic T. gondii Type I Infection
Seven- to nine-week-old female outbred CD-1 mice (ICR-Harlan Sprague) were infected (n=46) intraperitoneally with 500 tachyzoites of T. gondii GT1 strain (type I, virulent) diluted in 200 μL of phosphate-buffered saline (PBS). For controls, un-infected mice received 200 μL of only the vehicle (PBS) intraperitoneally (n=18). To establish a chronic infection, both control and infected mice were treated with anti-T. gondii chemotherapy (sulfadiazine sodium) in drinking water (400 mg/L, Sigma) from day 5 to day 30 post-infection. This treatment is to control the proliferation of tachyzoites during the acute stage and to avoid animal death. Sulfadiazine is a competitive analogue of PABA and inhibits tachyzoite growth, but not encysted bradyzoites. The GT1 strain was maintained by passage in human fibroblast cells (HFF, ATCC SCRC-1041).
To determine the kinetics of MAG1 antibody generation in mice infected with the GT1 strain, sera samples from a pilot mouse cohort (n=10) were collected weekly from the tail vein between weeks 2 and 18 following infection. The development of MAG1 antibody was monitored using the MAG1 ELISA assay, which measures antibodies directed against cyst antigen MAG1 using synthetic peptides (MAG1_4 and MAG1_5). The MAG1 antibody level was reported from either MAG1-4 or MAG1-5 depending on whichever one was higher. T. gondii infection was also confirmed by measuring anti-T. gondii IgG using a commercial ELISA assay (VIR-ELISA, Viro-Immun Labor-Diagnostika, Oberursel Germany) modified as previously described. The primary antibody consisted of diluted serum (1:100) and secondary antibody was enzyme labeled anti-mouse IgG.
Mice were subjected to behavioral assessments between 12 and 21 weeks postinfection (wpi), at the time when mice are expected to have their maximum levels of MAG1 antibody. The tests were conducted in the following order: novelty-induced activity in the open-field, spatial working and recognition memory in the Y-maze, novel object recognition, and amphetamine-induced activity in the open field.
Novelty-induced activity was examined using activity chambers with infrared beams (San Diego Instruments Inc.), as previously described. General locomotor activity was recorded as the number of beams broken and exploration was assessed as the number of rears for a 30-min period.
As previously described, the test included two sessions to evaluate spatial working and recognition memory, respectively, in a Y-maze. In the first session we scored the number of alternations done by the mouse when all three arms were visited, without entering the same arm twice in a row. After 5 days, the second session was performed and consisted of two trials. During trial 1, one arm of the maze was blocked and a mouse was allowed to freely explore the two open arms for 5 min. After a 20-min delay, trial 2 began during which the block was removed and the mouse was allowed to freely explore all three open arms for 5 min. The percentage of time and visits into the novel (previously blocked) arm during the first 2 min of the 5-min trial was analyzed.
As previously described, mice were habituated for 3 days to an empty cage for 10 min each day. On day 4, each mouse was subjected to three successive (habituation, pretest and test) sessions. After each session, there was a retention interval of 1 h, during which the mouse was held in its home cage. During the pretest session, two identical objects (object A) were placed on opposite ends of the empty cage, and the mouse was allowed to freely explore the objects for 10 min. During the test session, one of the two familiar objects was replaced with a novel one (object B), and the mouse was allowed to freely explore the familiar and novel object for 5 min. After 24 h, mouse was returned to the empty cage, which now contained an entirely novel object, C, and one of the familiar objects, A, and was tested for a duration of 5 min. The exploratory preference was calculated as the time near the novel object divided by the total time near either object. All the test objects have been extensively validated previously to ensure that no intrinsic preferences or aversions exist and that the animals explore all the objects for similar durations.
Each mouse was subjected to three successive (habituation, saline injection and amphetamine injection) sessions. Briefly, mice were first placed into an open-field chamber (San Diego Instruments Inc.) with their baseline activity recorded for 30 min, then injected intraperitoneally with 0.9% saline with their locomotor activity recorded for 30 additional min. Finally, mice were injected with D-Amphetamine sulfate (Sigma, St. Louis, Mo.) at doses of 2.5 mg/kg body weight and recorded for a final 60 min.
Mice that completed the behavioral testing were sacrificed between 22 and 24 wpi by cervical dislocation and then decapitation. The whole brain was removed and the prefrontal cortex and striata were rapidly dissected on ice and stored at −80° C. for subsequent experiments. For neurochemical analysis, one side of the striatum was snap frozen in liquid nitrogen, and stored at −80° C. Upon sacrifice, blood samples were collected and serum was isolated.
Total RNA was extracted using the miRNEasy kit (Qiagen). Reverse transcription was performed using either Multiscribe reverse transcriptase and random primers (Applied Biosystems, Foster City, Calif., USA) to generate cDNA, or the Multiscribe miRNA Reverse Transcription kit (Applied Biosystems) using miRNA-specific primers to produce miRNA. Quantitative PCR was performed using inventoried miRNA assays (Applied Biosystems) with standard ABI protocols and reagents, as previously described. The fold changes between groups were evaluated using relative quantization (delta Ct method) with b-actin as an endogenous mRNA control and RNU48 as an endogenous miRNA control. All the qPCR analyses were repeated at least three times to confirm differences in the expression levels and only results consistent across all three analyses were considered valid. For the mouse striatum, gene expression of miR-132 was measured. For the mouse cortex, expressions of BAG1 and SAG1 were measured.
Biogenic amine concentrations of striatal tissues were measured by high performance liquid chromatography with electrochemical detection (HPLC-ECD), as previously described. Data were normalized to protein concentrations (ng neurotransmitters/μg protein).
The levels of 23 different cytokines and chemokines were measured in the prefrontal cortex of the mouse brain using Bio-Plex multiplex assay (Bio-Rad) according to the manufacturer's instructions.
In a separate cohort of the same infection model, mice were sacrificed at 20 wpi and brains were removed and cut sagitally along the midline. For each mouse, half of the brain was homogenized in 400 μl of PBS containing 0.2% Triton X-100. A 1:2000 dilution of DAPI and a 1:200 dilution of fluorescein Dolichos biflorus agglutinin were added to an aliquot of the brain suspension and incubated on ice for 5 minutes. The aliquot was examined using a fluorescent microscope, and the number of brain cysts was determined in six (for mice with high MAG1 level) or twelve (for mice with low MAG1 or without MAG1 level) samples of 8 μl suspension per each brain homogenate at 400× magnification. All numbers reported correspond to the numbers obtained for the half-brain multiplied by 2 for comparison with published data for the whole brain. The size of the cyst was determined using the maximum diameter of the cyst.
Data are presented as means SEM. Based on the antibody profiles, mice were divided into five groups for analysis: control, mice with MAG high (IgG+/MAG1+high), mice with MAG1 low (IgG+/MAG1+low), mice without MAG (IgG+/MAG1−), and mice being exposed but didn't develop any antibodies (IgG−/MAG1−). Initial analysis showed that results were normally distributed. Generally, behavior and qPCR data were analyzed by one-way ANOVA. For monitoring body weight and amphetamine-induced activity, a repeated measures ANOVA was applied with group as the between-subject factor and time as the within-subjects factor. Further post hoc Bonferroni testing was conducted to explore any significant main effects resulting from the ANOVAs. Correlation analysis between MAG1 antibody level and measured parameters was performed using two-tailed Spearman's correlation coefficient (r). The correlation analysis only involved samples which are considered MAG1 seropositive. The effect of cytokine on behavior responses was examined by logistic regression analysis with and without adjusting for serological status (T. gondii and MAG1 antibody). Variables included in the regression models were selected based on known risk factors for T. gondii infection.
Statistical analyses were conducted in SPSS (Version 21.0, SPSS, Chicago, Ill., USA), GraphPad Prism V5.02 (GraphPad Software Inc., La Jolla, Calif., USA) and STATA version 12 (STATA Corp LP, College Station, Tex., U.S.A.). Significance was denoted as p<0.05.
All references, including publications, patent applications, and patents, cited herein are hereby incorporated by reference to the same extent as if each reference were individually and specifically indicated to be incorporated by reference and were set forth in its entirety herein.
The use of the terms “a” and “an” and “the” and similar referents in the context of describing the invention (especially in the context of the following claims) are to be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. The terms “comprising,” “having,” “including,” and “containing” are to be construed as open-ended terms (i.e., meaning “including, but not limited to,”) unless otherwise noted. Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., “such as”) provided herein, is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention unless otherwise claimed. No language in the specification should be construed as indicating any non-claimed element as essential to the practice of the invention.
Preferred embodiments of this invention are described herein, including the best mode known to the inventors for carrying out the invention. Variations of those preferred embodiments may become apparent to those of ordinary skill in the art upon reading the foregoing description. The inventors expect skilled artisans to employ such variations as appropriate, and the inventors intend for the invention to be practiced otherwise than as specifically described herein. Accordingly, this invention includes all modifications and equivalents of the subject matter recited in the claims appended hereto as permitted by applicable law. Moreover, any combination of the above-described elements in all possible variations thereof is encompassed by the invention unless otherwise indicated herein or otherwise clearly contradicted by context.
This application is a Continuation of U.S. patent application Ser. No. 16/095,777, filed Oct. 23, 2018, which is a 35 U.S.C. § 371 U.S. national entry of International Application PCT/US2017/029784, having an international filing date of Apr. 27, 2017, which claims the benefit of U.S. Provisional Application No. 62/328,818, filed Apr. 28, 2016, the content of each of the aforementioned applications is herein incorporated by reference in their entirety.
| Number | Date | Country | |
|---|---|---|---|
| 62328818 | Apr 2016 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 16095777 | Oct 2018 | US |
| Child | 17019645 | US |
