Patent Grant
8426617
This application is the US national phase of international application PCT/EP2005/013694 filed 20 Dec. 2005 which designated the U.S. and claims benefit of EP 04030432.1, dated 22 Dec. 2004, the entire content of which is hereby incorporated by reference.
The present invention relates to the (stereoselective) hydrogenation of a compound of the formula II with at least one carbon-carbon double bond, especially to the (stereoselective) hydrogenation of isoprenoids, non-cyclic sesquiterpenes, tocomonoenols, tocodienols, tocotrienols or any derivatives thereof, as well as to the (stereoselective) hydrogenation of parts/extracts of plant oils containing such tocotrienols or derivatives thereof, in the presence of a chiral Ir complex as the catalyst, whereby preferably one stereoisomer is manufactured in an excess.
In the prior art there exists no general method for the asymmetric hydrogenation of trisubstituted olefins bearing no functional group in near proximity, that means olefins in which the carbon atoms of the olefinic double bond are spaced from the functional group(s) by two or more CH2-groups could not be stereoselective hydrogenated so far. A “functional group” is understood as a group consisting of an aromatic residue or groups containing heteroatoms like O, N, S, P or similar. Examples of corresponding compounds which are useful for the synthesis of optically active tocopherols (vitamin E) are tocotrienols, unsaturated isoprenoids like geranylacetone or farnesene acid alkyl esters. Therefore, there is a need to provide catalysts for such stereoselective hydrogenation.
Surprisingly it was found that chiral Ir complexes, especially those containing P—N ligand systems, are suitable for that purpose. Such catalysts were until now only known for the stereoselective hydrogenation of aromatic compounds (see A. Pfaltz et al., Adv. Synth. Catal. 2003, 345 (1+2), 33-43; F. Menges, A. Pfaltz, Adv. Synth. Catal. 2002, 344 (1), 40-44; J. Blankenstein, A. Pfaltz, Angew. Chem. Int. Ed. 2001, 40 (23), 4445-4447; A. Pfaltz, Chimia 2004, 58 (1+2), 49-50).
Thus, one aspect of the invention refers to a process for the manufacture of at least one compound of the formula I
wherein the position labelled with the asterisk is an asymmetry center and
R1 is selected from the group consisting of linear C1-3-alkyl, C5-7-cycloalkyl, hydroxyl, hydroxyalkyl (alkyl=C1-4-alkyl), oxoalkyl (alkyl=C1-4-alkyl), alkylcarbonyl (alkyl=C1-4-alkyl), alkoxycarbonyl (alkoxy=linear C1-4-alkoxy) and a group of the formula
with R2 being a hydroxyl group or a protected hydroxyl group, and R3 and R4 being independently from each other hydrogen or methyl, and n being an integer from 1 to 10, preferably from 1 to 3,
comprising the step of
hydrogenating a compound of the formula II
wherein at least one carbon-carbon double bond is present, and wherein the dotted lines represent the possible positions of such (facultative) carbon-carbon double bonds; and R1 and n are as above,
in the presence of a chiral Ir complex as the catalyst.
Preferably in such process one stereoisomer of the compound I is manufactured in excess. If a compound of formula II with only one prochiral center is used preferably one enantiomer is manufactured in excess. The stereoselectivity of the hydrogenation can be controlled by appropriate choive of the catalyst.
Starting Material
Examples of the compounds of the formula II are those presented in
IIa1=(E)-Dihydrogeranylacetone, IIa2=(Z)-dihydronerylacetone, IIa3=(E)-geranylacetone, IIa4=(Z)-nerylacetone, IIb=(all-E)-farnesol; IIc=(all-E)-farnesene acid ethyl ester, (S)-XII=(2S,3′E,7′E)-tocotrienol and derivatives thereof, (R)-XII=(2R,3′E,7′E)-tocotrienol and derivatives thereof, (S)-XIII=(2S,3′E,7′E)-tocomono- and -dienols with the dotted lines indicating the possible positions of the one or two double bond(s), (R)-XIII=(2R,3′E,7′E)-tocomono- and -dienols with the dotted lines indicating the possible positions of the one or two double bond(s).
Preferably the compound of the formula II is an isoprenoid, a non-cyclic sesquiterpene, a tocomonoenol, a tocodienol or a tocotrienol.
An isoprenoid is an oligo(isoprene) or a poly(isoprene) and derivatives thereof which contain at least one carbon-carbon double bond. Preferably the carbon-carbon double bond has the E configuration.
The tocomonoenol, the tocodienol and/or the tocotrienol is of the formula XIII,
wherein the dotted bonds are optional and at least one of the dotted bonds is present,
and wherein R2 is a hydroxyl group or a protected hydroxyl group and R3 and R4 are independently from each other hydrogen or methyl.
Compound XIII thus encompasses (3′E)-tocomonoenols, (7′E)-tocomonoenols, (11′)-tocomonoenols, (3′E,7′E)-tocodienols, (3′E,11′)-tocodienols, (7′E,11′)-tocodienols, as well as (3′E,7′E)-tocotrienols.
Concerning the Substituent R2 in Formulae I, II and XIII:
R2 is a hydroxyl group or a protected hydroxyl group. The hydroxyl group can be protected as ether, ester, or acetal.
Examples of ethers and acetals are the methylether, the methoxymethylether, the methoxyethylether and the tetrahydropyranyl ether, as well as compounds where R2 is ethoxyethyl or methoxyethoxyethyl.
Examples of esters are the acetic acid ester and esters with other carbonic acids such as formic acid ester, succinic acid monoester (or derivatives), propionic acid ester, benzoic acid ester and palmitic acid ester.
Preferably R2 is a protected hydroxyl group, whereby the hydroxyl group is protected as ether or ester, more preferably as ester, especially preferably R2 is acetyloxy.
In another aspect the present invention is also concerned with a process for the manufacture of a hydrogenated part or extract of a plant oil, preferably of palm oil, comprising the step of hydrogenating the part or extract of the plant oil comprising at least a tocotrienol or derivative thereof in the presence of a chiral Ir complex as the catalyst. That means in the present invention such “a part or extract of the plant oil comprising at least a tocotrienol or derivative thereof” is also encompassed by the term “compound of the formula II with at least one carbon-carbon double bond”.
The expression “part of a plant oil” encompasses any untreated or treated part of the plant oil, any concentrated part as well as the whole plant oil itself. “treated” means chemically treated such as distilled or extracted or thermally treated.
Preferably the edible plant oil is treated in such a way that a part is obtained where the tocotrienols originally contained in the edible plant oil are enriched (“concentrate”). This part of the edible plant oil can be per se not edible.
Examples of plant oils are any edible plant oils known to the person skilled in the art. Especially preferred is palm oil which contains beside small amounts of α- and γ-tocopherol large amounts of tocotrienols.
In a preferred embodiment of the invention the tocotrienol or the derivative thereof is hydrogenated to a tocopherol (derivative), preferably to a highly stereoisomerically enriched (all-R)-tocopherol (derivative).
Catalyst
Suitable catalyst for the process of the present invention are Ir complexes with chiral organic ligands, especially those disclosed by A. Pfaltz et al. in Adv. Synth. Catal. 2003, 345 (1+2), 33-43; by F. Menges and A. Pfaltz in Adv. Synth. Catal. 2002, 344 (1), 40-44; by J. Blankenstein and A. Pfaltz in Angew. Chem. Int. Ed. 2001, 40 (23), 4445-4447, by A. Pfaltz in Chimia 2004, 58 (1+2), 49-50 and in U.S. Pat. No. 6,632,954.
Suitable catalysts are especially the Ir complexes of the formula III, IV, V, VI, VII, VIII, IX, X, XI or XV and their enantiomers
wherein R, X1, X2, X3, X4, X5, X6, X7, X8, X9, X10, X11, X12, X13, X14, X15, X16, X17, X18, X19, X20, X21 and X22 are independently from each other hydrogen, C1-4-alkyl, C5-7-cycloalkyl,
phenyl (optionally substituted with one to three C1-4-alkyl, C1-4-alkoxy and/or C1-4-perfluoroalkyl groups), benzyl, 1-naphthyl, or ferrocenyl,
the anion Y is a low coordinating anion, n is 1 or 2, and
wherein “o-Tol” means ortho-tolyl, “Ph” means phenyl, “TBDMS” means tert-butyl-di-methylsilyl, “p-Tol” means para-tolyl, and “BArF” means tetra(3,5-bis(trifluoromethyl)-phenyl)borate.
Suitable catalysts are also the corresponding Ir complexes and their enantiomers, in which the cyclooctadiene ligand is replaced by olefins, e.g. ethene, norbornadiene.
Preferred chiral Ir complexes suitable for the process of the present invention are Ir complexes of the formulae III to XI, wherein
R, X1, X2, X3, X3, X5, X6, X7, X8, X9, X10, X11, X12, X13, X14, X15, X16, X17, X18, X19, X20, X21 and X22 are independently from each other hydrogen, C1-4-alkyl, C5-7-cycloalkyl, phenyl (optionally substituted with one to three C1-4-alkyl, C1-4-alkoxy and/or C1-4-perfluoroalkyl groups), benzyl, 1-naphthyl, or ferrocenyl, and
the anion Y is a weakly coordinating anion such as PF6−, SbF6−, BArF−, BF4−, F3C—SO3−, ClO4−, tetra(perfluoroaryl)borate or tetra(perfluoroalkyloxy)aluminate whereby the perfluoroaryl is a phenyl substituted with 1 to 5 perfluoro-C1-4-alkyl groups and the perfluoroalkyloxy has 1 to 4 carbon atoms.
Especially preferred are Ir complexes of the formulae III to XI and XV, wherein
R, X1, X2, X3, X4, X5, X6, X7, X8, X9, X10, X11, X12, X13, X14, X15, X16, X17, X18, X19, X20, X21 and X22 are independently from each other hydrogen, methyl, ethyl, iso-propyl, n-butyl, iso-butyl, tert-butyl, cyclohexyl, phenyl, benzyl, o-tolyl, m-tolyl, 4-methoxyphenyl, 4-trifluoromethylphenyl, 3,5-di-tert-butylphenyl, 3,5-dimethoxyphenyl, 1-naphthyl, or ferrocenyl, and
the anion Y is tetra(perfluoroaryl)borate or tetra(perfluoroalkyloxy)aluminate whereby the perfluoroaryl is a phenyl substituted with 1 to 3 perfluoro-C1-4-alkyl groups and the perfluoroalkyloxy has 1 to 4 carbon atoms.
More preferred chiral Ir complexes suitable for the process of the present invention are Ir complexes of the formulae III to XI, and XV, wherein
R, X5, X14 and X20, X21, and X22 are independently from each other hydrogen, iso-propyl, tert-butyl, phenyl, 3,5-di-tert-butylphenyl or ferrocenyl,
X1 and X2, as well as X7 and X8, as well as X9 and X10 and X15 and X16 are independently from each other phenyl, o-tolyl, cyclohexyl or iso-propyl, preferably X1 and X2 as well as X7 and X8 or X9 and X10 or X15 and X16 are the same,
X3 and X4 as well as X11 and X12 as well as X17 and X18 are independently from each other methyl, ethyl, n-butyl, iso-butyl or benzyl, preferably X3 and X4, X11 and X12, X17 and X18 are the same,
X6 is hydrogen or methyl,
X13 and X19 are independently from each other phenyl, cyclohexyl, 4-methoxyphenyl, 3,5-dimethoxyphenyl, 4-trifluoromethylphenyl, benzyl, m-tolyl or 1-naphthyl,
Y is tetra(3,5-bis(trifluoromethyl)phenyl)borate [B(3,5-C6H3(CF3)2)4]− or tetra(perfluorotert-butyloxy)aluminate [Al(OC(CF3)3)4]− and n is as defined earlier.
Even more preferred chiral Ir complexes suitable for the process of the present invention are Ir complexes of the formulae III to XI, and XV, wherein
R is hydrogen, iso-propyl or tert-butyl,
X1 and X2 are independently from each other phenyl, o-tolyl, cyclohexyl or iso-propyl, preferably X1 and X2 are the same,
X3 and X4 are independently from each other benzyl or iso-butyl, preferably X3 and X4 are the same,
X5 is phenyl, 3,5-di-tert-butylphenyl or ferrocenyl,
X6 is hydrogen or methyl,
X7 and X8 are independently from each other phenyl or cyclohexyl, preferably X7 and X8 are the same,
X9 and X10 are independently from each other phenyl or o-tolyl, preferably X9 and X10 are the same,
X11 and X12 are independently from each other methyl, ethyl or n-butyl, preferably X11 and X12 are the same,
X13 is phenyl, cyclohexyl, 4-methoxyphenyl, 3,5-dimethoxyphenyl, 4-trifluoromethylphenyl, benzyl, m-tolyl or 1-naphthyl,
X14 is iso-propyl or tert-butyl,
X15 and X16 are independently from each other phenyl or o-tolyl, preferably X15 and X16 are the same,
X17 and X18 are independently from each other methyl or n-butyl, preferably X17 and X18 are the same,
X19 is phenyl or 1-naphthyl,
X20 is iso-propyl or tert-butyl,
X21 and X22 are
Y is tetra(3,5-bis(trifluoromethyl)phenyl)borate [B(3,5-C6H3(CF3)2)4]− or tetraperfluorotert-butyloxyaluminate [Al(OC(CF3)3)4]− and n is as defined earlier.
The most preferred chiral Ir complexes suitable for the process of the present invention are the Ir complexes of the formulae III to XI presented in the
“Cy”=cyclohexyl, “Bn”=benzyl, “i-Bu”=iso-butyl, “n-Bu”=n-butyl, “t-Bu”=tert-butyl, “Fc”=ferrocenyl, “o-Tol”=o-tolyl, “p-Tol”=p-tolyl, “i-Pr”=iso-propyl, “Me”=methyl, “Ph”=phenyl, “TBDMS”=tert-butyl-dimethyl silyl, “BArF” is tetra(3,5-bis(trifluoromethyl)phenyl)borate [B(3,5-C6H3(CF3)2)4]−.
Reaction Conditions
In the hydrogenation process of the present invention the amount of the catalyst is conveniently from about 0.05 to about 5 mol %, preferably from about 0.09 to about 2.5 mol %, more preferably from about 0.1 to about 2.0 mol-%, based on the amount of the compound of the formula II.
Preferred examples of halogenated aliphatic hydrocarbons are mono- or polyhalogenated linear, branched or cyclic C1- to C15-alkanes. Especially preferred examples are mono- or polychlorinated or -brominated linear, branched or cyclic C1- to C15-alkanes. More preferred are mono- or polychlorinated linear, branched or cyclic to C1- to C15-alkanes. Most preferred are dichloromethane, 1,2-dichloroethane, toluene 1,1,1-trichloroethane, chloroform, and methylene bromide. Further, toluene, benzene, and chlorobenzene come into consideration.
The reaction may be carried out under solvent free conditions, or in presence of one or more of the solvents mentioned above. The concentration of the reactants in the solution is not critical.
The reaction is conveniently carried out at an absolute pressure of hydrogen from about 1 to about 100 bar, preferably at an absolute pressure of hydrogen from about 20 to about 75 bar. The reaction temperature is conveniently between about 0 to about 100° C., preferably between about 10 to about 40° C.
The sequence of addition of the reactants and solvent is not critical.
In a first preferred embodiment of the present invention, (Z)-nerylacetone or (E)-geranylacetone or any mixture thereof is hydrogenated in the presence of a chiral Ir complex selected from the group consisting of catalyst A2 (see
In a second preferred embodiment of the present invention (E)-farnesol is hydrogenated in the presence of the chiral Ir complex E1 (see
In a third preferred embodiment of the present invention (E)-farnesene acid ethyl ester is hydrogenated in the presence of the chiral Ir complex B1 (see
In a fourth preferred embodiment of the present invention (2R,3′E,7′E)-α-tocotrienyl acetate is hydrogenated in the presence of the chiral Ir complex E1 (see
When the chiral Ir complex E2 or E15 (see
In a fifth preferred embodiment of the present invention (2S,3′E,7′E)-α-tocotrienyl acetate is hydrogenated in the presence of a chiral Ir complex selected from the group consisting of E3, E4, E6, E8, E9, E10, E11, E12, E13, E14 (all
In a sixth preferred embodiment of the present invention (2R,3′E,7′E)-γ-tocotrienyl acetate is hydrogenated in the presence of the chiral Ir complex C1 (see
Particular catalysts for use in the present invention are the chiral complexes represented by the formulae E1 to E15.
Finally the present invention is also directed to the use of a chiral Ir complex, especially one of the formulae III to XI as described above, as catalyst for the (stereoselective) hydrogenation of a compound selected from the group consisting of isoprenoids, non-cyclic sesquiterpenes, tocomonoenols, tocodienols and tocotrienols.
The invention is illustrated further by the following examples.
The following abbreviations are used:
“TLC”=thin layer chromatography; “GC”=gas chromatography; “GC-MS”=gas chromatography—mass spectrometry; “HPLC”=high pressure/performance liquid chromatography.
The asterisks in the 13C/1H-NMR parts mean that the signs could not be assigned unambiguously to a carbon/proton and the ones labelled with the asterisks are interchangeable.
The enantiomeric excess concerning the R-enantiomer is calculated as follows
[(amount of R-enantiomer minus amount of S-enantiomer)/(amount of R-enantiomer plus amount of S-enantiomer)]×100.
The enantiomeric excess concerning the R-enantiomer is calculated as follows
[(amount of R-enantiomer minus amount of S-enantiomer)/(amount of R-enantiomer plus amount of S-enantiomer)]×100.
General Remarks
All starting materials were prepared by DSM Nutritional Products, Lalden/Sisseln, Switzerland: (E)-Geranylacetone, 99.2% (GC); (Z)-nerylacetone, 97.6% (GC); (E)-dihydrogeranylacetone, 99.2% (GC); (Z)-dihydronerylacetone, 98.9% (GC); (all-E)-farnesol, 97.7% (GC); (2E,6E)-farnesene acid ethyl ester, 99.0% (GC); (2E,6Z)-farnesene acid ethyl ester, 78.2% (GC), contains 1.4% of the (6E) isomer and 17.6% of another not known isomer (GC-MS); (R,E,E)-alpha-tocotrienol acetate, ca. 99%; (S,E,E)-alpha-tocotrienyl acetate, ca. 99%; (R,E,E)-gamma-tocotrienyl acetate (prepared by total synthesis) 99.7% (HPLC); (S,E,E)-gamma-tocotrienyl acetate (prepared by total synthesis), 99.8% (HPLC). Reference compounds: (all-rac)-alpha-Tocopherol, 99.6% (GC); (all-rac)-alpha-tocopheryl acetate, 97.7% (GC); (all-rac)-alpha-tocopheryl methyl ether, 97.8% (GC); (all-rac)-gamma-tocopherol, 96.8% (GC); (R,R,R)-gamma-tocopheryl acetate, ca. 99% (GC); (all-rac)-gamma-tocopheryl methyl ether, 97.9% (GC).
If not stated otherwise, the GC analyses were performed with an Agilent 6890 GC FID on a CP-Sil-88 (Chrompack, Netherlands) 50 m×0.25 mm column. The carrier gas was hydrogen with 90 kPa. The samples were injected as 0.3% solutions in dichloromethane with a split ratio of 1/30. The injector was held at 250° C. whereas the oven temperature was programmed from 110-200° C. at 0.5° C./min, the detector was at 250° C.
In case of complete conversion a derivative of the hydrogenated product was prepared to determine the distribution of the stereoisomers.
Hydrogenated ketones or aldehydes e.g. were reacted with L- or D-trimethylsilyl diisopropyltartrat (shortly “L-3”, or “D-3”) in the presence of trimethylsilyl triflate [Si(CH3)3(OSO2CF3)] to the diastereomeric ketals and acetals, respectively. With the aid of achiral gas chromatography the ratio of the diastereomers could be determined and thus the selectivity of the stereoselective hydrogenation determined indirectly. (See also A. Knierzinger, W. Walther, B. Weber, T. Netscher, Chimia 1989, 43, 163-164; A. Knierzinger, W. Walther, B. Weber, R. K. Müller, T. Netscher, Helvetica Chimica Acta 1990, 73, 1087-1107)
For the determination of the ratio of the diastereoisomers of the prepared tocopheryl acetates these were first reduced to the corresponding tocopherols with LiAlH4 and then reacted with dimethyl sulphate to the tocopheryl methyl ethers. There were obtained four diastereoisomers. Their ratio was determined via achiral gas chromatography. (See also W. Walther, T. Netscher, Chirality 1996, 8, 397-401.)
The stereoisomeric composition of tocopherols (methyl ether derivatives) was also checked by chiral HPLC (Chiracel OD, 250×4.6 mm, solvent n-hexane, detection at 220 nm) in the case of the 2-(R)-isomers.
If examples were carried out at “room temperature”, this indicates that the reaction was carried out at a temperature of from about 20° C. to about 30° C.
Procedure 1
In an autoclave 0.25 mmol of the substrate, 1 mol-% of the Ir complex and 1.25 ml of absolute dichloromethane were put. The autoclave was closed and a pressure of 50 bar of hydrogen was applied. Under stirring the reaction solution was kept at room temperature for two hours. Afterwards the pressure was released and the solvent removed. For determining the conversion the raw product was analysed by GC without any further purification. If the reaction was complete, the product was converted into a derivative which enabled the determination of the stereoisomeric composition as illustrated further below in more detail, e.g., by converting a ketone to the (+)-L-diisopropyltartrate acetal and the (−)-D-diisopropyltartrate acetal, respectively.
The hydrogenation was carried out according to procedure 1, whereby 0.25 mmol of the substrate and 1 mol-% of the Ir-catalyst were used. The following substrates were used:
(E)-Dihydrogeranylacetone [=(E)-6,10-dimethylundec-5-ene-2-one] (49.1 mg), (Z)-dihydronerylacetone [=(Z)-6,10-dimethylundec-5-ene-2-one] (49.1 mg), (E)-geranylacetone [=(E)-6,10-dimethylundeca-5,9-diene-2-one] (48.6 mg) or (Z)-nerylacetone [=(Z)-6,10-dimethylundeca-5,9-dien-2-on] (48.6 mg).
1H-NMR (400.1 MHz, CDCl3) of the product: δ=0.85 (d, 3J=6.6 Hz, 3 H, —CH(CH3)—), 0.86 (d, 3J=6.6 Hz, 6 H, —CH(CH3)2), 1.10 (m, 4 H, 2 CH2), 1.26 (m, 4 H, 2 CH2), 1.39 (m, 1 H, CH), 1.54 (m, 3 H, CH2, CH), 2.13 (s, 3 H, —C(O)—CH3), 2.40 (t, 3J=7.6 Hz, 2 H, —C(O)—CH2—).—GC: Optima 5-Amin, 100 kPa He, temperature program: 100° C. (3 min), 2° C./min, 155° C. (O), 20° C./min, 250° C. (5 min); solvent: n-heptane; tR [Ia]=27.3 min, tR [IIa1]=28.1 min, tR [IIa2]=27.0 min, tR [IIa3]=30.3 min, tR [IIa4]=29.2 min.
The results are presented in the following tables 1 to 6:
The hydrogenation was carried out according to procedure 1, whereby 55.6 mg (0.25 mmol) of (2E,6E)-farnesol [=(2E,6E)-3,7,11-Trimethyldodeca-2,6,10-trien-1-ol] and 1 mol-% of Ir catalyst were used.
1H-NMR (400.1 MHz, CDCl3) of the product: δ=0.84 (d, 3J=6.8 Hz, 3 H, *CH—CH3), 0.86 (d, 3J=6.8 Hz, 6 H, CH(CH3)2), 0.89 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 1.0-1.42 (m, 14 H, 6 CH2, 2 CH), 1.55 (m, 3 H, CH2, CH), 3.68 (m, 2 H, CH2—OH).—GC: Restek Rtx-1701, 60 kPa He, temperature program: 50° C. (0 min), 10° C./min, 250° C. (10 min); solvent: n-heptane; tR [3,7,11-trimethyldodecane-1-ol]=18.5 min, tR [IIb]=19.8 min.
The results are presented in the following table 7:
The term “ee (3S)” denotes a value which is calculated for quantifying the extent of enantiomeric purity at C-3, omitting stereochemical information on C-7 as follows: ee (3S)=[(3S7R+3S7S) minus (3R7S+3R7R)] divided by [sum of all four stereoisomers (3S7R+3S7S+3R7S+3R7R)].
The term “ee (7S)” denotes a value which is calculated for quantifying the extent of enantiomeric purity at C-7, omitting stereochemical information on C-3 as follows: ee (7S)=[(3S7S+3R7S) minus (3R7R+3S7R)] divided by [sum of all four stereoisomers (3S7S+3R7S+3R7R+3S7R)].
The hydrogenation was carried out according to procedure 1, whereby 66.1 mg (0.25 mmol) of (2E,6E)-farnesene acid ethyl ester [=(2E,6E)-3,7,11-trimethyl-dodeca-2,6,10-triene acid ethyl ester] and 1 mol-% of the Ir-catalyst were used.
1H-NMR (400.1 MHz, CDCl3): δ=0.84 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 0.86 (d, 3J=6.6 Hz, 6 H, CH(CH3)2), 0.93 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 1.0-1.4 (m, 13 H, 6 CH2, CH(CH3)2), 1.25 (t, 3J=7.0 Hz, 3 H, O—CH2—CH3), 1.52 (m, 1 H, *CH), 1.94 (m, 1 H, *CH), 2.07 (ddt, 2J=14.7 Hz, 3J=8.1 Hz, 4J=1.5 Hz, 1 H, CH2—COOEt), 2.28 (ddt, 2J=14.7 Hz, 3J=6.1 Hz, 4J=1.8 Hz, 1 H, CH2—COOEt), 4.13 (q, 3J=7.0 Hz, 2 H, O—CH2—CH3).—GC: Restek Rtx-1701, 60 kPa He, temperature program: 50° C. (0 min), 10° C./min, 250° C. (10 min); solvent: n-heptane; tR [Ic]=19.1 min, tR [IIc]=21.0 min.
The results are presented in the following table 8:
The term “ee C-3 (S)” denotes a value which is calculated for quantifying the extent of enantiomeric purity at C-3, omitting stereochemical information on C-7 as follows: ee C-3 (S)=[(3S7R+3S7S) minus (3R7S+3R7R)] divided by [sum of all four stereoisomers (3S7R+3S7S+3R7S+3R7R)].
The term “ee C-3 (R)” denotes a value which is calculated for quantifying the extent of enantiomeric purity at C-3, omitting stereochemical information on C-7 as follows: ee C-3 (R)=[(3R7R+3R7S) minus (3S7S+3S7R)] divided by [sum of all four stereoisomers (3R7R+3R7S+3S7S+3S7R)].
The term “ee C-7 (S)” denotes a value which is calculated for quantifying the extent of enantiomeric purity at C-7, omitting stereochemical information on C-3 as follows: ee C-7 (S)=[(3S7S+3R7S) minus (3R7R+3S7R)] divided by [sum of all four stereoisomers (3S7S+3R7S+3R7R+3S7R)].
The term “ee C-7 (R)” denotes a value which is calculated for quantifying the extent of enantiomeric purity at C-7, omitting stereochemical information on C-3 as follows: ee C-7 (R)=[(3S7R+3R7R) minus (3R7S+3S7S)] divided by [sum of all four stereoisomers (3S7R+3R7R+3R7S+3S7S)].
Procedure 2: Converting an Ester such as 3,7,11-Trimethyl-dodecanoic Acid Ethyl Ester into a Derivative for Determination of the Stereoisomeric Composition
0.25 mmol of the isolated ester were dissolved in 2 ml of absolute tetrahydrofuran and mixed with 66 mg (1.75 mmol, 7 mol equivalents) of LiAlH4. The grey suspension was stirred for one hour at room temperature. Afterwards 5 ml of destillated water were added under ice cooling and stirring was continued for another 10 minutes. The resulting phases were separated and the aqueous phase was extracted twice with diethyl ether. The combined organic extracts were dried over MgSO4 and the solvent was removed. The isolated alcohol, e.g., 3,7,11-trimethyldodecan-1-o1 was further reacted to the corresponding aldehyde, e.g., 3,7,11-trimethyl-dodecanal without any purification.
Oxidation of 3,7,11-Trimethyldodecane-1-ol to 3,7,11-Trimethyl-dodecanal
The isolated alcohol was dissolved under Ar atmosphere in 1 ml of absolute dichloromethane. 60 mg of pyridinium chlorochromate were added. The brown suspension was stirred until the turn-over was complete (ca. 3 hours) at room temperature. Then the suspension was diluted with 3 ml of diethyl ether and filtrated. The solvent was removed and the raw product purified by column chromatography on silica gel (solvent: diethyl ether). The solvent was removed. Thin layer chromatography: starting material: Rf-value=0.22; product: Rf-value=0.67 (SiO2, n-hexane/ethyl acetate (9:1); development with basic KMnO4 solution). For the preparation of the corresponding acetal the raw aldehyde was immediately further reacted.
1H-NMR (400.1 MHz, CDCl3): δ=0.84 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 0.86 (d, 3J=6.6 Hz, 6 H, CH(CH3)2), 0.97 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 1.02-1.42 (m, 13 H, 6 CH2, CH), 1.52 (m, 1 H, *CH), 1.96 (m, 1 H, *CH), 2.14 (ddd, 2J=14.9 Hz, 3J=5.8 Hz, 3J=2.0 Hz, 1 H, —CH2—CHO), 2.35 (ddd, 2J=14.9 Hz, 3J=8.1 Hz, 3J=2.0 Hz, 1 H, —CH2—CHO), 9.75 (t, 3J=2.3 Hz, 1 H, CHO).
Acetalisation of 6,10-Dimethylundecane-2-One to Di-(2-Methyl-Ethyl)-(4R,5R)-2-[4,8-Dimethylnonyl]-2-Methyl-1,3-Dioxolane-4,5-Dicarboxylate
To 0.25 mmol of (6R)-6,10-dimethylundecane-2-one and (6S)-6,10-dimethylundecane-2-one, respectively, were added 142 mg (0.38 mmol, 1.5 mol equivalents) of (2R,6R)-bissilylether (L-3) in 1 ml of absolute dichloromethane under Ar atmosphere. The reaction mixture was cooled to −78° C. At this temperature 20 μL (0.1 mmol, 0.4 mol equivalents) of trimethylsilyl triflate were added. After 15 minutes the cooling bath was removed and the reaction mixture stirred for 12 hours at room temperature. Afterwards 0.14 ml (1.0 mmol) of triethyl amine were added and stirring continued for further 10 minutes. Then the solvents were removed in high vacuum. The residue was dissolved in diethyl ether, filtered over silica gel and the solvent evaporated. For determination of the diastereomeric excess the raw product was analyzed via GC without any further purification.
TLC: Rf-value=0.27 (SiO2, n-hexane/ethyl acetate 9:1); Rf-value (Ia)=0.32.—GC: achiral column: CP-Sil-88 (50 m, 0.25 mm, 0.25 μm), 100% cyanopropylpolysiloxan; carrier gas: hydrogen (90 kPa); split injector (1:30), injection temperature: 250° C.; FID detector, detection temperature: 250° C.; temperature program: 147° C. (isotherm); Solvent: dichloromethane; tR (4R,5R,4′S-acetal)=129.3 min, tR (4R,5R,4′R-acetal)=130.7 min.
1H-NMR (400.1 MHz, CDCl3): δ=0.84 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 0.85 (d, 3J=6.6 Hz, 6 H, CH(CH3)2), 1.00-1.57 (m, 12 H, 6 CH2), 1.29 (d, 3J=6.3 Hz, 12 H, CO2CH(CH3)2), 1.44 (s, 3 H, acetal-CH3), 1.69 (m, 2 H, CH), 4.63 (d, 3J=6.3 Hz, 1 H, *CH (tartrate)), 4.67 (d, 3J=6.3 Hz, 1 H, *CH (tartrate)), 5.13 (sept., 3J=6.3 Hz, 2 H, 2 CO2CH(CH3)2).—
Acetalisation of 3,7,11-Trimethyl-Dodecane-1-Carbaldehyde to di-(2-Methylethyl)-(4R,5R/4S,5S)-[2,6,10-Trimethylundecyl]-1,3-di-Oxolan-4,5-Dicarboxylate
To 71 mg L-3 and D-3, respectively, were added under Ar atmosphere 0.5 ml of a solution of 0.25 mmol of freshly prepared 3,7,11-trimethyl-dodecane-1-carbaldehyde and 1.0 ml of absolute dichloromethane. The reaction mixture was cooled to −78° C. At this temperature 10 μL (0.05 mmol, 0.4 mol equivalents) of trimethylsilyl triflate were added dropwise. The further proceeding corresponds to the proceeding described above for the preparation of acetal of 6,10-dimethylundecane-2-one.
TLC: Rf-value (product)=0.25 (SiO2, n-hexane/ethyl acetate 9:1); Rf-value (starting material)=0.45.—GC: achiral column: CP-Sil-88 (50 m, 0.25 mm, 0.25 μm), 100% cyanopropylpolysiloxan; carrier gas: hydrogen (90 kPa), split injector (1:30), injection temperature: 250° C., FID detector, detection temperature: 250° C.; temperature program: 110° C.→200° C. with a heating rate of 0.5° C./min; tR (L-2′S,6′R-acetal)=144.3 min, tR {(L-2′R,6′S-acetal)+(L-2′S,6′S-acetal)}=145.0 min, tR (L-2′R,6′R-acetal)=145.6 min, or tR (D-2′R,6′S-acetal)=144.3 min, tR {(D-2′S,6′R-acetal)+(D-2′R,6′R-acetal)}=145.0 min, tR (D-2′S,6′S-acetal)=145.6 min.
1H-NMR (400.1 MHz, CDCl3): δ=0.83 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 0.86 (d, 3J=6.8 Hz, 6 H, CH(CH3)2), 0.94 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 1.00-1.65 (m, 16 H, 7×CH2, 2×*CH), 1.28 (d, 3J=6.3 Hz, 12 H, 2 CO2CH(CH3)2), 4.56 (d, 3J=4.3 Hz, 1 H, *CH (tartrate), 4.65 (d, 3J=4.3 Hz, 1 H, *CH (tartrate), 5.11 (sept., 3J=6.3 Hz, 1 H, CO2CH(CH3)2), 5.12 (sept., 3J=6.3 Hz, 1 H, CO2CH(CH3)2), 5.30 (t, 3J=5.05 Hz, 1 H, acetal-H).—
Stereoselective Hydrogenation of (2R)- and (2S)-α-Tocotrienyl Acetate, as well as of (2R)- and (2S)-γ-Tocotrienyl Acetate
Hydrogenation according to procedure 1, whereby 23.4 mg (0.05 mmol) of starting material and 1 mol-% of Ir catalyst (based on the amount of starting material) in 0.5 ml of absolute dichloromethane were used. Used starting materials: (2R,3′E,7′E)-α-tocotrienyl acetate, (2S,3′E,7′E)-α-tocotrienyl acetate.
Determination of the turn-over via 1H-NMR; (2R/2S,3′E,7′E)-α-tocotrienyl acetate: 5.13 (m, 3 H, 3 alken-CH).—1H-NMR (400.1 MHz, CD2Cl2): δ=0.87 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 0.88 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 0.89 (d, 3J=6.6 Hz, 6 H, CH(CH3)2), 1.06-1.63 (m, 21 H, 9 CH2, 3 CH), 1.26 (s, 3 H, O—*C—CH3), 1.82 (m, 2 H, O—*C—CH2), 1.97 (s, 3 H, Ph—CH3), 2.01 (s, 3 H, Ph—CH3), 2.10 (s, 3 H, Ph—CH3), 2.31 (OC(O)CH3), 2.62 (m, 2 H, CH2 (cycl.)).
The results are presented in the following tables 9, 10, 11 and 12:
If the catalyst E15 (enantiomer to Ir complex E14) was used (2R,3′E,7′E)-α-tocotrienyl acetate was hydrogenated to (2R,4′R,8′R)-tocopheryl acetate in a yield of 90% (see table 12).
Hydrogenation according to procedure 1, whereby 0.05 mmol (22.7 mg) of the starting material and 1 mol-% (based on the amount of starting material) of the Ir catalyst in 0.5 ml of absolute dichoromethane were used. Used starting materials: (2R,3′E,7′E)-γ-tocotrienyl acetate, (2S,3′E,7′E)-γ-tocotrienyl acetate.
The conversion was determined via 1H-NMR; (2R/2S,3′E,7′E)-γ-tocotrienyl acetate: 5.13 (m, 3 H, 3 Alken-CH). 1H-NMR (400.1 MHz, CD2Cl2): δ=0.86 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 0.87 (d, 3J=6.6 Hz, 6 H, CH(CH3)2), 0.88 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 1.02-1.68 (m, 21 H, 9 CH2, 3 CH), 1.28 (s, 3 H, O—*C—CH3), 1.76 (dt, 2J=13.5 Hz, 3J=6.6 Hz, 1 H, O—*C—CH2), 1.80 (dt, 2J=13.5 Hz, 3J=6.6 Hz, 1 H, O—*C—CH2), 2.01 (s, 3 H, Ph—CH3), 2.12 (s, 3 H, Ph—CH3), 2.27 (s, 3 H, OC(O)CH3), 2.72 (m, 2 H, CH2 (cycl.), 6.56 (s, 1 H, ar. CH).
The results are presented in the following tables 13 and 14:
For determination of the stereoisomeric composition the tocopheryl acetates were converted into tocopherols and tocopherol methyl ethers as follows:
Reduction of the Tocopheryl Acetates to the Corresponding Tocopherols
Preparation of (2R)-α-Tocopherol and (2S)-α-Tocopherol
Synthesis according to procedure 2, whereby 23.7 mg (0.05 mmol) of the starting material and 13 mg (0.35 mmol) of LiAlH4 were used in 1 ml of absolute tetrahydrofuran. Used starting materials: (2R)-α-tocopheryl acetate and (2S)-α-tocopheryl acetate.
1H-NMR (400.1 MHz, CD2Cl2): δ=0.86 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 0.87 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 0.88 (d, 3J=6.6 Hz, 6 H, CH(CH3)2), 1.02-1.63 (m, 21 H, 9 CH2, 3 CH), 1.23 (s, 3 H, O—*C—CH3), 1.79 (m, 2 H, O—*C—CH2), 2.10 (br s, 6 H, 2 Ph—CH3), 2.14 (s, 3 H, Ph—CH3), 2.60 (m, 2 H, CH2 (cycl.)), 4.28 (br s, 1 H, OH).
Preparation of (2R)-γ-Tocopherol and (2S)-γ-Tocopherol
Synthesis according to procedure 2, whereby 22.9 mg (0.05 mmol) of starting material and 13 mg (0.35 mmol) of LiAlH4 were used in 1 ml of absolute tetrahydrofuran. Used starting materials: (2R)-γ-tocopheryl acetate and (2S)-γ-tocopheryl acetate.
1H-NMR (400.1 MHz, CD2Cl2): δ=0.86 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 0.87 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 0.88 (d, 3J=6.6 Hz, 6 H, CH(CH3)2), 1.04-1.63 (m, 21 H, 9 CH2, 3 CH), 1.28 (s, 3 H, O—*C—CH3), 1.75 (m, 2 H, O—*C—CH2), 2.09 (s, 3 H, Ph—CH3), 2.11 (s, 3 H, Ph—CH3), 2.67 (m, 2 H, CH2 (cycl.), 4.35 (br s, 1 H, OH), 6.36 (s, 1 H, ar. CH).
Procedure 3
Preparation of the Methyl Ether of α- and γ-Tocopherol
0.25 mmol of the isolated α- or γ-tocopherol (raw product) were dissolved under an Ar atmosphere in 1 ml of absolute dimethoxyethan. 0.2 ml (2.5 mmol) of a 50 weight-% aqueous KOH solution were added dropwise. After stirring for 10 minutes 0.12 ml (1.25 mmol) of dimethyl sulfate were added dropwise. Afterwards the reaction mixture was stirred for one hour at room temperature. After complete turn-over the solvent was evaporated. The residue was stirred in 5 ml of distilled water and 10 ml of n-hexane for 5 minutes. The organic and the aqueous phase were separated. The aqueous phase was extracted with 10 ml of n-hexane. The combined organic phases were dried over MgSO4 and the solvent evaporated. The obtained raw product was analyzed without any further purification via GC to determine the ratio of the diastereomers.
Preparation of (2R)-α-Tocopheryl Methyl Ether and (2S)-α-Tocopheryl Methyl Ether
Synthesis according to procedure 3; GC: achiral column: CP-Sil-88 (50 m, 0.25 mm, 0.25 μm), 100% cyanopropylpolysiloxane; carrier gas: hydrogen (90 kPa); split injector (1:30), injection temperature: 280° C.; FID detector, detection temperature: 250° C.; temperature program: 170° C. (isotherm); solvent: ethyl acetate; tR of the products: tR (2R,4′R,8′S)=144.5 min, tR (2R,4′R,8′R)=146.2 min, tR (2R,4S,8R)=148.4 min, tR (2R,4′S,8′S) =150.8 min bzw. tR (2S,4′S,8′R)=144.5 min, tR (2S,4′S,8′S)=146.2 min, tR (2S,4′R,8′S)=148.4 min, tR (2S,4′R,8′R)=150.8 min.
1H-NMR (400.1 MHz, CD2Cl2): δ=0.86 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 0.87 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 0.88 (d, 3J=6.6 Hz, 6 H, CH(CH3)2), 1.03-1.65 (m, 21 H, 9 CH2, 3 CH), 1.25 (s, 3 H, O—*C—CH3), 1.75 (m, 2 H, O—*C—CH2), 2.09 (br s, 9 H, 3 Ph—CH3), 2.72 (m, 2 H, CH2 (cycl.), 3.74 (s, 3 H, —O—CH3).
Preparation of (2R)-γ-Tocopheryl Methyl Ether and (2S)-γ-Tocopheryl Methyl Ether
Preparation according to procedure 3; GC: achiral column: CP-Sil-88 (50 m, 0.25 mm, 0.25 μm), 100% cyanopropylpolysiloxane; carrier gas: hydrogen (90 kPa); split injector (1:30), injection temperature: 280° C.; FID detector, detection temperature: 250° C.; temperature program: 170° C. (isotherm); solvent: ethyl acetate; tR of the products: tR (2R,4′R,8′S)=126.0 min, tR (2R,4′R,8′R)=127.5 min, tR (2R,4′S,8′R)=129.5 min, tR (2R,4′S,8′S)=132.0 min; and tR (2S,4′S,8′R)=126.0 min, tR (2S,4′S,8′S)=127.5 min, tR (2S,4′R,8′S)=129.5 min, tR (2S,4′R,8′R)=132.0 min.
1H-NMR (400.1 MHz, CD2Cl2): δ=0.87 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 0.88 (d, 3J=6.6 Hz, 3 H, *CH—CH3), 0.89 (d, 3J=6.6 Hz, 6 H, CH(CH3)2), 1.03-1.65 (m, 21 H, 9 CH2, 3 CH), 1.29 (s, 3 H, O—*C—CH3), 1.77 (m, 2 H, O—*C—CH2), 2.09 (s, 3 H, Ph—CH3), 2.10 (s, 3 H, Ph—CH3), 2.72 (m, 2 H, CH2 (cycl.), 3.74 (s, 3 H, —O—CH3), 6.43 (s, 1 H, ar. CH).
(2R,3′E,7′E)-γ-Tocotrienyl acetate (22.7 mg, 0.05 mmol), catalyst (5×10−7 mol, 1 mol %) and dichloromethane (0.5 ml) were added under nitrogen to a 2 ml glass vial containing a magnetic stir bar and placed in an autoclave. The autoclave was pressurized to 50 bar with H2 and the solution stirred at 700 rpm for 2 hours. The pressure was then carefully released and the reaction mixture concentrated under reduced pressure. Hexane (1 ml) was added and the mixture filtered through a 0.2 μm syringe filter. The hexane solution was then concentrated to give 23 mg (100%) of (2R)-γ-tocopheryl acetate (>98% 2R,4′R,8′R; <0.5% 2R,4′R,8′S; <0.5% 2R,4′S,8′R; <0.5% 2R,4′S,8′S) as an oil.
| Number | Date | Country | Kind |
|---|---|---|---|
| 04030432 | Dec 2004 | EP | regional |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/EP2005/013694 | 12/20/2005 | WO | 00 | 8/8/2007 |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO2006/066863 | 6/29/2006 | WO | A |
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| 7534921 | Jäkel et al. | May 2009 | B2 |
| 20010047110 | Kenzo et al. | Nov 2001 | A1 |
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| 0 565 975 | Oct 1993 | EP |
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| 20080039638 A1 | Feb 2008 | US |
