AURONE DERIVATIVE-CONTAINING COMPOSITION FOR DIAGNOSIS

TECHNICAL FIELD

The present invention relates to an aurone derivative-containing composition for use in diagnosis. The composition of the present invention may be used for diagnosing amyloid-related diseases such as Alzheimer's disease.

BACKGROUND ART

With the rapid aging of population in recent years, increases in the number of patients with diseases associated with dementia such as Alzheimer's disease (AD) have become one of serious social problems. Currently, the Hasegawa Scale, ADAS, and MMSE are available as methods for clinical diagnosis of AD. All of these methods quantitatively evaluate the decreased cognitive function in an individual suspected of AD and are commonly used. In addition, image diagnosis methods (MRI, CT, etc.) are supplementarily used. However, these diagnostic methods are inadequate for definite diagnosis of AD, and development of senile plaques and neurofibrils needs to be confirmed in biopsy of the brain before death or histopathological examination of the brain after death to make definite diagnosis. Accordingly, it is difficult to diagnose AD at an early stage before occurrence of an extensive brain damage by the current diagnostic methods.

Although some reports have shown biological diagnostic markers for AD so far, no clinically practical technique has yet been developed. Under such circumstances, social demand for early diagnosis of AD is high, and urgent development of a method therefor is eagerly anticipated.

Senile plaques are the most characteristic brain lesion of AD, and the major component thereof is amyloid β protein having a β-sheet structure. Imaging of senile plaques from out of the body is considered to lead to the establishment of an effective diagnostic method for AD, and such imaging requires a probe compound that specifically binds to amyloid β protein. So far, several derivatives containing congo red or thioflavine T as a parent structure have been reported as probe compounds (Japanese Patent Unexamined Publications No. 2004-250407 and No. 2004-250411; Klunk W. E. et al., Annals of Neurology Vol. 55, No. 3, March 2004, 306-319), but these compounds suffer from many problems including low specificity to amyloid β protein, low permeability through the blood-brain barrier, and slow clearance due to their nonspecific binding in the brain.

Therefore, these reported compounds have not yet been put into practical use for diagnosis of diseases associated with accumulation of amyloid at present. The present inventors have found that flavone derivatives, chalcone derivatives, styrylchromone derivatives and coumarin derivatives specifically bind to amyloid β protein and already filed a patent application based on this finding (Patent Document 1).

[Patent Document 1]WO/2006/057323

DISCLOSURE OF THE INVENTION
Problem for Solution by the Invention

The present invention was accomplished under the technical background described above, and an object thereof is to provide a compound having the following three properties: high binding specificity to amyloid β protein, high permeability through the blood-brain barrier, and rapid clearance from sites without senile plaques in the brain.

Means to Solve the Problem

As a result of intensive and extensive researches toward the solution of the above problem, the present inventors have found that aurone derivatives have an excellent property as a probe compound for imaging amyloid β protein, and achieved the present invention based on this finding.

Specifically, the present invention provides the following (1) to (11).

(1) A composition for diagnosing amyloid-related diseases, comprising a compound represented by general formula (I):

wherein X represents O or NH; R¹, R², R³and R⁴are the same or different and each represent a hydrogen atom, a halogen atom, a group represented by a formula —(CH₂CH₂O)_n—F where n is an integer from 1 to 10 or a group represented by a formula —(CH₂CH₂O)_n—OH where n is an integer from 1 to 10; and R⁵, R⁶, R⁷, R⁸and R⁹are the same or different and each represent a hydrogen atom, a halogen atom, a dimethylamino group, a methylamino group, an amino group, a methoxy group, a hydroxyl group, a group represented by a formula —(CH₂CH₂O), —F where n is an integer from 1 to 10 or a group represented by a formula —(CH₂CH₂O)_n—OH where n is an integer from 1 to 10; or the compound labeled with a radionuclide, or a pharmaceutically acceptable salt of the compound or the labeled compound.

(2) The composition according to (1) above, wherein X in general formula (I) is O.

(3) The composition according to (1) or (2) above, wherein R²in general formula (I) is a fluorine atom, an iodine atom or a group represented by a formula —(CH₂CH₂O)_n—F where n is an integer from 1 to 10.

(4) The composition according to (1) or (2) above, wherein R²in general formula (I) is a fluorine atom, an iodine atom or a group represented by a formula —(CH₂CH₂O)_n—F where n is an integer from 1 to 10; and R¹, R³and R⁴each represent a hydrogen atom.

(5) The composition according to any one of (1) to (4) above, wherein R⁷in general formula (I) is a fluorine atom, an iodine atom, a dimethylamino group, a methylamino group, an amino group, a methoxy group, a hydroxyl group, a group represented by a formula —(CH₂CH₂O)_n—F where n is an integer from 1 to 10 or a group represented by a formula —(CH₂CH₂O)_n—OH where n is an integer from 1 to 10.

(6) The composition according to any one of (1) to (4) above, wherein R⁷in general formula (I) is a fluorine atom, an iodine atom, a dimethylamino group, a methylamino group, an amino group, a methoxy group, a hydroxyl group, a group represented by a formula —(CH₂CH₂O)_n—F where n is an integer from 1 to 10 or a group represented by a formula —(CH₂CH₂O)_n—OH where n is an integer from 1 to 10; and R⁵, R⁶, R⁸and R⁹each represent a hydrogen atom.

(7) The composition according to any one of (1) to (6) above, wherein the radionuclide is a positron-emitting radionuclide.

(8) The composition according to any one of (1) to (6) above, wherein the radionuclide is a γ-ray-emitting radionuclide.

(9) The composition according to any one of (1) to (8) above, wherein the amyloid-related disease is Alzheimer's disease.

(10) A method of screening for therapeutic or prophylactic agents for amyloid-related diseases, comprising a step of administering a test substance to an amyloid-related disease model animal, a step of administering the composition according to any one of (1) to (9) above to the model animal, and a step of examining the distribution or quantity of the compound represented by general formula (I) contained in the brain of the model animal.

(11) A method of evaluating therapeutic or prophylactic agents for amyloid-related diseases, comprising a step of administering a therapeutic or prophylactic agent for amyloid-related diseases to an amyloid-related disease model animal, a step of administering the composition according to any one of (1) to (9) above to the model animal, and a step of examining the distribution or quantity of the compound represented by general formula (I) contained in the brain of the model animal.

EFFECT OF THE INVENTION

Since the compound represented by general formula (I) has high binding specificity to amyloid β protein and a property of being rapidly eliminated from sites other than senile plaques in the brain, the compound is useful in diagnosing Alzheimer's disease.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows a process of synthesizing aurone derivatives with dimethylamino group or the like (1). Numbers in this figure correspond to compound numbers.

FIG. 2 shows a process of synthesizing aurone derivatives with dimethylamino group or the like (2). Numbers in this figure correspond to compound numbers.

FIG. 3 shows a process of synthesizing aurone derivatives with dimethylamino group or the like (3). Numbers in this figure correspond to compound numbers.

FIG. 4 shows the binding properties of [¹²⁵I]-labeled aurone derivatives to Ab(1-42) aggregate (1).

FIG. 5 shows the binding properties of [¹²⁵I]-labeled aurone derivatives to Ab(1-42) aggregate (2).

FIG. 6 shows the results of autoradiography and immunostaining on hippocampal sections from an Alzheimer's disease patient.

FIG. 7 shows a process of synthesizing aurone derivatives having hydroxyethoxy group or the like (1). Numbers in this figure correspond to compound numbers.

FIG. 8 shows a process of synthesizing aurone derivatives having hydroxyethoxy group or the like (2). Numbers in this figure correspond to compound numbers.

FIG. 9 shows the results of immunostaining on brain sections from an Alzheimer's disease model mouse (1: compound 26; 2: thioflavin S; 3: anti-amyloid antibody).

FIG. 10 shows the results of autoradiography and immunostaining on hippocampal sections from an Alzheimer's disease patient (A: autoradiography with [¹²⁵I]26; B: positive site in immunostaining with anti-amyloid β(1-42) antibody; C: negative site in immunostaining with anti-arnyloid β(1-42) antibody).

BEST MODE FOR CARRYING OUT THE INVENTION

Hereinbelow, the present invention will be described in details.

The term “halogen atom” used herein refers to, for example, fluorine atom, chlorine atom, bromine atom or iodine atom.

In a formula —(CH₂CH₂O)_n—F, n is preferably an integer from 1 to 3.

In a formula —(CH₂CH₂O)_n—OH, n is preferably an integer from 1 to 3.

In general formula (I), X is preferably O.

In general formula (I), R¹is preferably a hydrogen atom.

In general formula (I), R²is preferably a fluorine atom, an iodine atom or a group represented by a formula —(CH₂CH₂O)_n—F where n is an integer from 1 to 10; more preferably, R²is a fluorine atom, an iodine atom, a 2-fluoroethoxy group, a 2-(2-fluoroethoxy)ethoxy group or a 2-{2-(2-fluoroethoxy)ethoxy}ethoxy group.

In general formula (I), R³is preferably a hydrogen atom.

In general formula (I), R⁴is preferably a hydrogen atom.

In general formula (I), R⁵is preferably a hydrogen atom.

In general formula (I), R⁶is preferably a hydrogen atom.

In general formula (I), R⁷is preferably a fluorine atom, an iodine atom, a dimethylamino group, a methylamino group, an amino group, a methoxy group, a hydroxyl group, a group represented by a formula —(CH₂CH₂O)_n—F where n is an integer from 1 to 10 or a group represented by a formula —(CH₂CH₂O)_n—OH where n is an integer from 1 to 10; more preferably, R⁷is a fluorine atom, an iodine atom, a dimethylamino group, a methylamino group, an amino group, a methoxy group, a hydroxyl group, a 2-fluoroethoxy group, a 2-(2-fluoroethoxy)ethoxy group, a 2-{2-(2-fluoroethoxy)ethoxy}ethoxy group, a 2-hydroxyethoxy group, a 2-(2-hydroxyethoxy)ethoxy group or a 2-{2-(2-hydroxyethoxy)ethoxy}ethoxy group; still more preferably, R⁷is a dimethylamino group, a methylamino group, a 2-hydroxyethoxy group, a 2-(2-hydroxyethoxy)ethoxy group or a 2-{2-(2-hydroxyethoxy)ethoxy}ethoxy group.

In general formula (I), R⁸is preferably a hydrogen atom.

In general formula (I), R⁹is preferably a hydrogen atom.

Representative examples of those compounds represented by general formula (I) are shown in the following Tables. In the Tables, “Me” represents methyl group; “FX” represents 2-fluoroethoxy group; “FX2” represents 2-(2-fluoroethoxy)ethoxy group; “FX3” represents 2-{2-(2-fluoroethoxy)ethoxy}ethoxy group; “HOX” represents 2-hydroxyethoxy group; “HOX2” represents 2-(2-hydroxyethoxy)ethoxy group; and “HOX3” represents 2-{2-(2-hydroxyethoxy)ethoxy}ethoxy group.

TABLE 1

X
R¹
R²
R³
R⁴
R⁵
R⁶
R⁷
R⁸
R⁹

I-1
O
H
H
F
H
H
H
H
H
H

I-2
O
H
H
F
H
H
H
NH₂
H
H

I-3
O
H
H
F
H
H
H
OMe
H
H

I-4
O
H
H
F
H
H
H
OH
H
H

I-5
O
H
H
I
H
H
H
H
H
H

I-6
O
H
H
I
H
H
H
NH₂
H
H

I-7
O
H
H
I
H
H
H
OMe
H
H

I-8
O
H
H
I
H
H
H
OH
H
H

I-9
O
H
H
FX
H
H
H
H
H
H

I-10
O
H
H
FX
H
H
H
NH₂
H
H

I-11
O
H
H
FX
H
H
H
OMe
H
H

I-12
O
H
H
FX
H
H
H
OH
H
H

I-13
O
H
H
FX2
H
H
H
H
H
H

I-14
O
H
H
FX2
H
H
H
NH₂
H
H

I-15
O
H
H
FX2
H
H
H
OMe
H
H

I-16
O
H
H
FX2
H
H
H
OH
H
H

I-17
O
H
H
FX3
H
H
H
H
H
H

I-18
O
H
H
FX3
H
H
H
NH₂
H
H

I-19
O
H
H
FX3
H
H
H
OMe
H
H

I-20
O
H
H
FX3
H
H
H
OH
H
H

I-21
O
H
H
H
H
H
H
F
H
H

I-22
O
H
H
H
H
H
H
I
H
H

I-23
O
H
H
H
H
H
H
FX
H
H

I-24
O
H
H
H
H
H
H
FX2
H
H

I-25
O
H
H
H
H
H
H
FX3
H
H

I-26
O
H
H
H
H
NMe₂
H
H
H
H

I-27
O
H
H
H
H
H
NMe₂
H
H
H

I-28
O
H
H
H
H
H
H
NMe₂
H
H

I-29
O
H
H
H
H
H
H
H
NMe₂
H

I-30
O
H
H
H
H
H
H
H
H
NMe₂

I-31
O
H
H
H
H
NHMe
H
H
H
H

I-32
O
H
H
H
H
H
NHMe
H
H
H

I-33
O
H
H
H
H
H
H
NHMe
H
H

I-34
O
H
H
H
H
H
H
H
NHMe
H

I-35
O
H
H
H
H
H
H
H
H
NHMe

I-36
O
F
H
H
H
NMe₂
H
H
H
H

I-37
O
H
F
H
H
NMe₂
H
H
H
H

I-38
O
H
H
F
H
NMe₂
H
H
H
H

I-39
O
H
H
H
F
NMe₂
H
H
H
H

I-40
O
F
H
H
H
H
NMe₂
H
H
H

I-41
O
H
F
H
H
H
NMe₂
H
H
H

I-42
O
H
H
F
H
H
NMe₂
H
H
H

I-43
O
H
H
H
F
H
NMe₂
H
H
H

I-44
O
F
H
H
H
H
H
NMe₂
H
H

I-45
O
H
F
H
H
H
H
NMe₂
H
H

I-46
O
H
H
F
H
H
H
NMe₂
H
H

I-47
O
H
H
H
F
H
H
NMe₂
H
H

I-48
O
F
H
H
H
H
H
H
NMe₂
H

I-49
O
H
F
H
H
H
H
H
NMe₂
H

I-50
O
H
H
F
H
H
H
H
NMe₂
H

I-51
O
H
H
H
F
H
H
H
NMe₂
H

I-52
O
F
H
H
H
H
H
H
H
NMe₂

I-53
O
H
F
H
H
H
H
H
H
NMe₂

I-54
O
H
H
F
H
H
H
H
H
NMe₂

I-55
O
H
H
H
F
H
H
H
H
NMe₂

I-56
O
F
H
H
H
NHMe
H
H
H
H

I-57
O
H
F
H
H
NHMe
H
H
H
H

I-58
O
H
H
F
H
NHMe
H
H
H
H

I-59
O
H
H
H
F
NHMe
H
H
H
H

I-60
O
F
H
H
H
H
NHMe
H
H
H

I-61
O
H
F
H
H
H
NHMe
H
H
H

I-62
O
H
H
F
H
H
NHMe
H
H
H

I-63
O
H
H
H
F
H
NHMe
H
H
H

I-64
O
F
H
H
H
H
H
NHMe
H
H

I-65
O
H
F
H
H
H
H
NHMe
H
H

I-66
O
H
H
F
H
H
H
NHMe
H
H

TABLE 2

I-67
O
H
H
H
F
H
H
NHMe
H
H

I-68
O
F
H
H
H
H
H
H
NHMe
H

I-69
O
H
F
H
H
H
H
H
NHMe
H

I-70
O
H
H
F
H
H
H
H
NHMe
H

I-71
O
H
H
H
F
H
H
H
NHMe
H

I-72
O
F
H
H
H
H
H
H
H
NHMe

I-73
O
H
F
H
H
H
H
H
H
NHMe

I-74
O
H
H
F
H
H
H
H
H
NHMe

I-75
O
H
H
H
F
H
H
H
H
NHMe

I-76
O
I
H
H
H
NMe₂
H
H
H
H

I-77
O
H
I
H
H
NMe₂
H
H
H
H

I-78
O
H
H
I
H
NMe₂
H
H
H
H

I-79
O
H
H
H
I
NMe₂
H
H
H
H

I-80
O
I
H
H
H
H
NMe₂
H
H
H

I-81
O
H
I
H
H
H
NMe₂
H
H
H

I-82
O
H
H
I
H
H
NMe₂
H
H
H

I-83
O
H
H
H
I
H
NMe₂
H
H
H

I-84
O
I
H
H
H
H
H
NMe₂
H
H

I-85
O
H
I
H
H
H
H
NMe₂
H
H

I-86
O
H
H
I
H
H
H
NMe₂
H
H

I-87
O
H
H
H
I
H
H
NMe₂
H
H

I-88
O
I
H
H
H
H
H
H
NMe₂
H

I-89
O
H
I
H
H
H
H
H
NMe₂
H

I-90
O
H
H
I
H
H
H
H
NMe₂
H

I-91
O
H
H
H
I
H
H
H
NMe₂
H

I-92
O
I
H
H
H
H
H
H
H
NMe₂

I-93
O
H
I
H
H
H
H
H
H
NMe₂

I-94
O
H
H
I
H
H
H
H
H
NMe₂

I-95
O
H
H
H
I
H
H
H
H
NMe₂

I-96
O
I
H
H
H
NHMe
H
H
H
H

I-97
O
H
I
H
H
NHMe
H
H
H
H

I-98
O
H
H
I
H
NHMe
H
H
H
H

I-99
O
H
H
H
I
NHMe
H
H
H
H

I-100
O
I
H
H
H
H
NHMe
H
H
H

I-101
O
H
I
H
H
H
NHMe
H
H
H

I-102
O
H
H
I
H
H
NHMe
H
H
H

I-103
O
H
H
H
I
H
NHMe
H
H
H

I-104
O
I
H
H
H
H
H
NHMe
H
H

I-105
O
H
I
H
H
H
H
NHMe
H
H

I-106
O
H
H
I
H
H
H
NHMe
H
H

I-107
O
H
H
H
I
H
H
NHMe
H
H

I-108
O
I
H
H
H
H
H
H
NHMe
H

I-109
O
H
I
H
H
H
H
H
NHMe
H

I-110
O
H
H
I
H
H
H
H
NHMe
H

I-111
O
H
H
H
I
H
H
H
NHMe
H

I-112
O
I
H
H
H
H
H
H
H
NHMe

I-113
O
H
I
H
H
H
H
H
H
NHMe

I-114
O
H
H
I
H
H
H
H
H
NHMe

I-115
O
H
H
H
I
H
H
H
H
NHMe

I-116
O
FX
H
H
H
NMe₂
H
H
H
H

I-117
O
H
FX
H
H
NMe₂
H
H
H
H

I-118
O
H
H
FX
H
NMe₂
H
H
H
H

I-119
O
H
H
H
FX
NMe₂
H
H
H
H

I-120
O
FX
H
H
H
H
NMe₂
H
H
H

I-121
O
H
FX
H
H
H
NMe₂
H
H
H

I-122
O
H
H
FX
H
H
NMe₂
H
H
H

I-123
O
H
H
H
FX
H
NMe₂
H
H
H

I-124
O
FX
H
H
H
H
H
NMe₂
H
H

I-125
O
H
FX
H
H
H
H
NMe₂
H
H

I-126
O
H
H
FX
H
H
H
NMe₂
H
H

I-127
O
H
H
H
FX
H
H
NMe₂
H
H

I-128
O
FX
H
H
H
H
H
H
NMe₂
H

I-129
O
H
FX
H
H
H
H
H
NMe₂
H

I-130
O
H
H
FX
H
H
H
H
NMe₂
H

I-131
O
H
H
H
FX
H
H
H
NMe₂
H

I-132
O
FX
H
H
H
H
H
H
H
NMe₂

I-133
O
H
FX
H
H
H
H
H
H
NMe₂

TABLE 3

I-134
O
H
H
FX
H
H
H
H
H
NMe₂

I-135
O
H
H
H
FX
H
H
H
H
NMe₂

I-136
O
FX
H
H
H
NHMe
H
H
H
H

I-137
O
H
FX
H
H
NHMe
H
H
H
H

I-138
O
H
H
FX
H
NHMe
H
H
H
H

I-139
O
H
H
H
FX
NHMe
H
H
H
H

I-140
O
FX
H
H
H
H
NHMe
H
H
H

I-141
O
H
FX
H
H
H
NHMe
H
H
H

I-142
O
H
H
FX
H
H
NHMe
H
H
H

I-143
O
H
H
H
FX
H
NHMe
H
H
H

I-144
O
FX
H
H
H
H
H
NHMe
H
H

I-145
O
H
FX
H
H
H
H
NHMe
H
H

I-146
O
H
H
FX
H
H
H
NHMe
H
H

I-147
O
H
H
H
FX
H
H
NHMe
H
H

I-148
O
FX
H
H
H
H
H
H
NHMe
H

I-149
O
H
FX
H
H
H
H
H
NHMe
H

I-150
O
H
H
FX
H
H
H
H
NHMe
H

I-151
O
H
H
H
FX
H
H
H
NHMe
H

I-152
O
FX
H
H
H
H
H
H
H
NHMe

I-153
O
H
FX
H
H
H
H
H
H
NHMe

I-154
O
H
H
FX
H
H
H
H
H
NHMe

I-155
O
H
H
H
FX
H
H
H
H
NHMe

I-156
O
FX2
H
H
H
NMe₂
H
H
H
H

I-157
O
H
FX2
H
H
NMe₂
H
H
H
H

I-158
O
H
H
FX2
H
NMe₂
H
H
H
H

I-159
O
H
H
H
FX2
NMe₂
H
H
H
H

I-160
O
FX2
H
H
H
H
NMe₂
H
H
H

I-161
O
H
FX2
H
H
H
NMe₂
H
H
H

I-162
O
H
H
FX2
H
H
NMe₂
H
H
H

I-163
O
H
H
H
FX2
H
NMe₂
H
H
H

I-164
O
FX2
H
H
H
H
H
NMe₂
H
H

I-165
O
H
FX2
H
H
H
H
NMe₂
H
H

I-166
O
H
H
FX2
H
H
H
NMe₂
H
H

I-167
O
H
H
H
FX2
H
H
NMe₂
H
H

I-168
O
FX2
H
H
H
H
H
H
NMe₂
H

I-169
O
H
FX2
H
H
H
H
H
NMe₂
H

I-170
O
H
H
FX2
H
H
H
H
NMe₂
H

I-171
O
H
H
H
FX2
H
H
H
NMe₂
H

I-172
O
FX2
H
H
H
H
H
H
H
NMe₂

I-173
O
H
FX2
H
H
H
H
H
H
NMe₂

I-174
O
H
H
FX2
H
H
H
H
H
NMe₂

I-175
O
H
H
H
FX2
H
H
H
H
NMe₂

I-176
O
FX2
H
H
H
NHMe
H
H
H
H

I-177
O
H
FX2
H
H
NHMe
H
H
H
H

I-178
O
H
H
FX2
H
NHMe
H
H
H
H

I-179
O
H
H
H
FX2
NHMe
H
H
H
H

I-180
O
FX2
H
H
H
H
NHMe
H
H
H

I-181
O
H
FX2
H
H
H
NHMe
H
H
H

I-182
O
H
H
FX2
H
H
NHMe
H
H
H

I-183
O
H
H
H
FX2
H
NHMe
H
H
H

I-184
O
FX2
H
H
H
H
H
NHMe
H
H

I-185
O
H
FX2
H
H
H
H
NHMe
H
H

I-186
O
H
H
FX2
H
H
H
NHMe
H
H

I-187
O
H
H
H
FX2
H
H
NHMe
H
H

I-188
O
FX2
H
H
H
H
H
H
NHMe
H

I-189
O
H
FX2
H
H
H
H
H
NHMe
H

I-190
O
H
H
FX2
H
H
H
H
NHMe
H

I-191
O
H
H
H
FX2
H
H
H
NHMe
H

I-192
O
FX2
H
H
H
H
H
H
H
NHMe

I-193
O
H
FX2
H
H
H
H
H
H
NHMe

I-194
O
H
H
FX2
H
H
H
H
H
NHMe

I-195
O
H
H
H
FX2
H
H
H
H
NHMe

I-196
O
FX3
H
H
H
NMe₂
H
H
H
H

I-197
O
H
FX3
H
H
NMe₂
H
H
H
H

I-198
O
H
H
FX3
H
NMe₂
H
H
H
H

I-199
O
H
H
H
FX3
NMe₂
H
H
H
H

I-200
O
FX3
H
H
H
H
NMe₂
H
H
H

TABLE 4

I-201
O
H
FX3
H
H
H
NMe₂
H
H
H

I-202
O
H
H
FX3
H
H
NMe₂
H
H
H

I-203
O
H
H
H
FX3
H
NMe₂
H
H
H

I-204
O
FX3
H
H
H
H
H
NMe₂
H
H

I-205
O
H
FX3
H
H
H
H
NMe₂
H
H

I-206
O
H
H
FX3
H
H
H
NMe₂
H
H

I-207
O
H
H
H
FX3
H
H
NMe₂
H
H

I-208
O
FX3
H
H
H
H
H
H
NMe₂
H

I-209
O
H
FX3
H
H
H
H
H
NMe₂
H

I-210
O
H
H
FX3
H
H
H
H
NMe₂
H

I-211
O
H
H
H
FX3
H
H
H
NMe₂
H

I-212
O
FX3
H
H
H
H
H
H
H
NMe₂

I-213
O
H
FX3
H
H
H
H
H
H
NMe₂

I-214
O
H
H
FX3
H
H
H
H
H
NMe₂

I-215
O
H
H
H
FX3
H
H
H
H
NMe₂

I-216
O
FX3
H
H
H
NHMe
H
H
H
H

I-217
O
H
FX3
H
H
NHMe
H
H
H
H

I-218
O
H
H
FX3
H
NHMe
H
H
H
H

I-219
O
H
H
H
FX3
NHMe
H
H
H
H

I-220
O
FX3
H
H
H
H
NHMe
H
H
H

I-221
O
H
FX3
H
H
H
NHMe
H
H
H

I-222
O
H
H
FX3
H
H
NHMe
H
H
H

I-223
O
H
H
H
FX3
H
NHMe
H
H
H

I-224
O
FX3
H
H
H
H
H
NHMe
H
H

I-225
O
H
FX3
H
H
H
H
NHMe
H
H

I-226
O
H
H
FX3
H
H
H
NHMe
H
H

I-227
O
H
H
H
FX3
H
H
NHMe
H
H

I-228
O
FX3
H
H
H
H
H
H
NHMe
H

I-229
O
H
FX3
H
H
H
H
H
NHMe
H

I-230
O
H
H
FX3
H
H
H
H
NHMe
H

I-231
O
H
H
H
FX3
H
H
H
NHMe
H

I-232
O
FX3
H
H
H
H
H
H
H
NHMe

I-233
O
H
FX3
H
H
H
H
H
H
NHMe

I-234
O
H
H
FX3
H
H
H
H
H
NHMe

I-235
O
H
H
H
FX3
H
H
H
H
NHMe

I-236
NH
H
H
F
H
H
H
H
H
H

I-237
NH
H
H
F
H
H
H
NH₂
H
H

I-238
NH
H
H
F
H
H
H
OMe
H
H

I-239
NH
H
H
F
H
H
H
OH
H
H

I-240
NH
H
H
I
H
H
H
H
H
H

I-241
NH
H
H
I
H
H
H
NH₂
H
H

I-242
NH
H
H
I
H
H
H
OMe
H
H

I-243
NH
H
H
I
H
H
H
OH
H
H

I-244
NH
H
H
FX
H
H
H
H
H
H

I-245
NH
H
H
FX
H
H
H
NH₂
H
H

I-246
NH
H
H
FX
H
H
H
OMe
H
H

I-247
NH
H
H
FX
H
H
H
OH
H
H

I-248
NH
H
H
FX2
H
H
H
H
H
H

I-249
NH
H
H
FX2
H
H
H
NH₂
H
H

I-250
NH
H
H
FX2
H
H
H
OMe
H
H

I-251
NH
H
H
FX2
H
H
H
OH
H
H

I-252
NH
H
H
FX3
H
H
H
H
H
H

I-253
NH
H
H
FX3
H
H
H
NH₂
H
H

I-254
NH
H
H
FX3
H
H
H
OMe
H
H

I-255
NH
H
H
FX3
H
H
H
OH
H
H

I-256
NH
H
H
H
H
H
H
F
H
H

I-257
NH
H
H
H
H
H
H
I
H
H

I-258
NH
H
H
H
H
H
H
FX
H
H

I-259
NH
H
H
H
H
H
H
FX2
H
H

I-260
NH
H
H
H
H
H
H
FX3
H
H

I-261
NH
H
H
H
H
NMe₂
H
H
H
H

I-262
NH
H
H
H
H
H
NMe₂
H
H
H

I-263
NH
H
H
H
H
H
H
NMe₂
H
H

I-264
NH
H
H
H
H
H
H
H
NMe₂
H

I-265
NH
H
H
H
H
H
H
H
H
NMe₂

I-266
NH
H
H
H
H
NHMe
H
H
H
H

I-267
NH
H
H
H
H
H
NHMe
H
H
H

TABLE 5

I-268
NH
H
H
H
H
H
H
NHMe
H
H

I-269
NH
H
H
H
H
H
H
H
NHMe
H

I-270
NH
H
H
H
H
H
H
H
H
NHMe

I-271
NH
F
H
H
H
NMe₂
H
H
H
H

I-272
NH
H
F
H
H
NMe₂
H
H
H
H

I-273
NH
H
H
F
H
NMe₂
H
H
H
H

I-274
NH
H
H
H
F
NMe₂
H
H
H
H

I-275
NH
F
H
H
H
H
NMe₂
H
H
H

I-276
NH
H
F
H
H
H
NMe₂
H
H
H

I-277
NH
H
H
F
H
H
NMe₂
H
H
H

I-278
NH
H
H
H
F
H
NMe₂
H
H
H

I-279
NH
F
H
H
H
H
H
NMe₂
H
H

I-280
NH
H
F
H
H
H
H
NMe₂
H
H

I-281
NH
H
H
F
H
H
H
NMe₂
H
H

I-282
NH
H
H
H
F
H
H
NMe₂
H
H

I-283
NH
F
H
H
H
H
H
H
NMe₂
H

I-284
NH
H
F
H
H
H
H
H
NMe₂
H

I-285
NH
H
H
F
H
H
H
H
NMe₂
H

I-286
NH
H
H
H
F
H
H
H
NMe₂
H

I-287
NH
F
H
H
H
H
H
H
H
NMe₂

I-288
NH
H
F
H
H
H
H
H
H
NMe₂

I-289
NH
H
H
F
H
H
H
H
H
NMe₂

I-290
NH
H
H
H
F
H
H
H
H
NMe₂

I-291
NH
F
H
H
H
NHMe
H
H
H
H

I-292
NH
H
F
H
H
NHMe
H
H
H
H

I-293
NH
H
H
F
H
NHMe
H
H
H
H

I-294
NH
H
H
H
F
NHMe
H
H
H
H

I-295
NH
F
H
H
H
H
NHMe
H
H
H

I-296
NH
H
F
H
H
H
NHMe
H
H
H

I-297
NH
H
H
F
H
H
NHMe
H
H
H

I-298
NH
H
H
H
F
H
NHMe
H
H
H

I-299
NH
F
H
H
H
H
H
NHMe
H
H

I-300
NH
H
F
H
H
H
H
NHMe
H
H

I-301
NH
H
H
F
H
H
H
NHMe
H
H

I-302
NH
H
H
H
F
H
H
NHMe
H
H

I-303
NH
F
H
H
H
H
H
H
NHMe
H

I-304
NH
H
F
H
H
H
H
H
NHMe
H

I-305
NH
H
H
F
H
H
H
H
NHMe
H

I-306
NH
H
H
H
F
H
H
H
NHMe
H

I-307
NH
F
H
H
H
H
H
H
H
NHMe

I-308
NH
H
F
H
H
H
H
H
H
NHMe

I-309
NH
H
H
F
H
H
H
H
H
NHMe

I-310
NH
H
H
H
F
H
H
H
H
NHMe

I-311
NH
I
H
H
H
NMe₂
H
H
H
H

I-312
NH
H
I
H
H
NMe₂
H
H
H
H

I-313
NH
H
H
I
H
NMe₂
H
H
H
H

I-314
NH
H
H
H
I
NMe₂
H
H
H
H

I-315
NH
I
H
H
H
H
NMe₂
H
H
H

I-316
NH
H
I
H
H
H
NMe₂
H
H
H

I-317
NH
H
H
I
H
H
NMe₂
H
H
H

I-318
NH
H
H
H
I
H
NMe₂
H
H
H

I-319
NH
I
H
H
H
H
H
NMe₂
H
H

I-320
NH
H
I
H
H
H
H
NMe₂
H
H

I-321
NH
H
H
I
H
H
H
NMe₂
H
H

I-322
NH
H
H
H
I
H
H
NMe₂
H
H

I-323
NH
I
H
H
H
H
H
H
NMe₂
H

I-324
NH
H
I
H
H
H
H
H
NMe₂
H

I-325
NH
H
H
I
H
H
H
H
NMe₂
H

I-326
NH
H
H
H
I
H
H
H
NMe₂
H

I-327
NH
I
H
H
H
H
H
H
H
NMe₂

I-328
NH
H
I
H
H
H
H
H
H
NMe₂

I-329
NH
H
H
I
H
H
H
H
H
NMe₂

I-330
NH
H
H
H
I
H
H
H
H
NMe₂

I-331
NH
I
H
H
H
NHMe
H
H
H
H

I-332
NH
H
I
H
H
NHMe
H
H
H
H

I-333
NH
H
H
I
H
NHMe
H
H
H
H

I-334
NH
H
H
H
I
NHMe
H
H
H
H

TABLE 6

I-335
NH
I
H
H
H
H
NHMe
H
H
H

I-336
NH
H
I
H
H
H
NHMe
H
H
H

I-337
NH
H
H
I
H
H
NHMe
H
H
H

I-338
NH
H
H
H
I
H
NHMe
H
H
H

I-339
NH
I
H
H
H
H
H
NHMe
H
H

I-340
NH
H
I
H
H
H
H
NHMe
H
H

I-341
NH
H
H
I
H
H
H
NHMe
H
H

I-342
NH
H
H
H
I
H
H
NHMe
H
H

I-343
NH
I
H
H
H
H
H
H
NHMe
H

I-344
NH
H
I
H
H
H
H
H
NHMe
H

I-345
NH
H
H
I
H
H
H
H
NHMe
H

I-346
NH
H
H
H
I
H
H
H
NHMe
H

I-347
NH
I
H
H
H
H
H
H
H
NHMe

I-348
NH
H
I
H
H
H
H
H
H
NHMe

I-349
NH
H
H
I
H
H
H
H
H
NHMe

I-350
NH
H
H
H
I
H
H
H
H
NHMe

I-351
NH
FX
H
H
H
NMe₂
H
H
H
H

I-352
NH
H
FX
H
H
NMe₂
H
H
H
H

I-353
NH
H
H
FX
H
NMe₂
H
H
H
H

I-354
NH
H
H
H
FX
NMe₂
H
H
H
H

I-355
NH
FX
H
H
H
H
NMe₂
H
H
H

I-356
NH
H
FX
H
H
H
NMe₂
H
H
H

I-357
NH
H
H
FX
H
H
NMe₂
H
H
H

I-358
NH
H
H
H
FX
H
NMe₂
H
H
H

I-359
NH
FX
H
H
H
H
H
NMe₂
H
H

I-350
NH
H
FX
H
H
H
H
NMe₂
H
H

I-361
NH
H
H
FX
H
H
H
NMe₂
H
H

I-362
NH
H
H
H
FX
H
H
NMe₂
H
H

I-363
NH
FX
H
H
H
H
H
H
NMe₂
H

I-364
NH
H
FX
H
H
H
H
H
NMe₂
H

I-365
NH
H
H
FX
H
H
H
H
NMe₂
H

I-366
NH
H
H
H
FX
H
H
H
NMe₂
H

I-367
NH
FX
H
H
H
H
H
H
H
NMe₂

I-368
NH
H
FX
H
H
H
H
H
H
NMe₂

I-369
NH
H
H
FX
H
H
H
H
H
NMe₂

I-370
NH
H
H
H
FX
H
H
H
H
NMe₂

I-371
NH
FX
H
H
H
NHMe
H
H
H
H

I-372
NH
H
FX
H
H
NHMe
H
H
H
H

I-373
NH
H
H
FX
H
NHMe
H
H
H
H

I-374
NH
H
H
H
FX
NHMe
H
H
H
H

I-375
NH
FX
H
H
H
H
NHMe
H
H
H

I-376
NH
H
FX
H
H
H
NHMe
H
H
H

I-377
NH
H
H
FX
H
H
NHMe
H
H
H

I-378
NH
H
H
H
FX
H
NHMe
H
H
H

I-379
NH
FX
H
H
H
H
H
NHMe
H
H

I-380
NH
H
FX
H
H
H
H
NHMe
H
H

I-381
NH
H
H
FX
H
H
H
NHMe
H
H

I-382
NH
H
H
H
FX
H
H
NHMe
H
H

I-383
NH
FX
H
H
H
H
H
H
NHMe
H

I-384
NH
H
FX
H
H
H
H
H
NHMe
H

I-385
NH
H
H
FX
H
H
H
H
NHMe
H

I-386
NH
H
H
H
FX
H
H
H
NHMe
H

I-387
NH
FX
H
H
H
H
H
H
H
NHMe

I-388
NH
H
FX
H
H
H
H
H
H
NHMe

I-389
NH
H
H
FX
H
H
H
H
H
NHMe

I-390
NH
H
H
H
FX
H
H
H
H
NHMe

I-391
NH
FX2
H
H
H
NMe₂
H
H
H
H

I-392
NH
H
FX2
H
H
NMe₂
H
H
H
H

I-393
NH
H
H
FX2
H
NMe₂
H
H
H
H

I-394
NH
H
H
H
FX2
NMe₂
H
H
H
H

I-395
NH
FX2
H
H
H
H
NMe₂
H
H
H

I-396
NH
H
FX2
H
H
H
NMe₂
H
H
H

I-397
NH
H
H
FX2
H
H
NMe₂
H
H
H

I-398
NH
H
H
H
FX2
H
NMe₂
H
H
H

I-399
NH
FX2
H
H
H
H
H
NMe₂
H
H

I-400
NH
H
FX2
H
H
H
H
NMe₂
H
H

I-401
NH
H
H
FX2
H
H
H
NMe₂
H
H

TABLE 7

I-402
NH
H
H
H
FX2
H
H
NMe₂
H
H

I-403
NH
FX2
H
H
H
H
H
H
NMe₂
H

I-404
NH
H
FX2
H
H
H
H
H
NMe₂
H

I-405
NH
H
H
FX2
H
H
H
H
NMe₂
H

I-406
NH
H
H
H
FX2
H
H
H
NMe₂
H

I-407
NH
FX2
H
H
H
H
H
H
H
NMe₂

I-408
NH
H
FX2
H
H
H
H
H
H
NMe₂

I-409
NH
H
H
FX2
H
H
H
H
H
NMe₂

I-410
NH
H
H
H
FX2
H
H
H
H
NMe₂

I-411
NH
FX2
H
H
H
NHMe
H
H
H
H

I-412
NH
H
FX2
H
H
NHMe
H
H
H
H

I-413
NH
H
H
FX2
H
NHMe
H
H
H
H

I-414
NH
H
H
H
FX2
NHMe
H
H
H
H

I-415
NH
FX2
H
H
H
H
NHMe
H
H
H

I-416
NH
H
FX2
H
H
H
NHMe
H
H
H

I-417
NH
H
H
FX2
H
H
NHMe
H
H
H

I-418
NH
H
H
H
FX2
H
NHMe
H
H
H

I-419
NH
FX2
H
H
H
H
H
NHMe
H
H

I-420
NH
H
FX2
H
H
H
H
NHMe
H
H

I-421
NH
H
H
FX2
H
H
H
NHMe
H
H

I-422
NH
H
H
H
FX2
H
H
NHMe
H
H

I-423
NH
FX2
H
H
H
H
H
H
NHMe
H

I-424
NH
H
FX2
H
H
H
H
H
NHMe
H

I-425
NH
H
H
FX2
H
H
H
H
NHMe
H

I-426
NH
H
H
H
FX2
H
H
H
NHMe
H

I-427
NH
FX2
H
H
H
H
H
H
H
NHMe

I-428
NH
H
FX2
H
H
H
H
H
H
NHMe

I-429
NH
H
H
FX2
H
H
H
H
H
NHMe

I-430
NH
H
H
H
FX2
H
H
H
H
NHMe

I-431
NH
FX3
H
H
H
NMe₂
H
H
H
H

I-432
NH
H
FX3
H
H
NMe₂
H
H
H
H

I-433
NH
H
H
FX3
H
NMe₂
H
H
H
H

I-434
NH
H
H
H
FX3
NMe₂
H
H
H
H

I-435
NH
FX3
H
H
H
H
NMe₂
H
H
H

I-436
NH
H
FX3
H
H
H
NMe₂
H
H
H

I-437
NH
H
H
FX3
H
H
NMe₂
H
H
H

I-438
NH
H
H
H
FX3
H
NMe₂
H
H
H

I-439
NH
FX3
H
H
H
H
H
NMe₂
H
H

I-440
NH
H
FX3
H
H
H
H
NMe₂
H
H

I-441
NH
H
H
FX3
H
H
H
NMe₂
H
H

I-442
NH
H
H
H
FX3
H
H
NMe₂
H
H

I-443
NH
FX3
H
H
H
H
H
H
NMe₂
H

I-444
NH
H
FX3
H
H
H
H
H
NMe₂
H

I-445
NH
H
H
FX3
H
H
H
H
NMe₂
H

I-446
NH
H
H
H
FX3
H
H
H
NMe₂
H

I-447
NH
FX3
H
H
H
H
H
H
H
NMe₂

I-448
NH
H
FX3
H
H
H
H
H
H
NMe₂

I-449
NH
H
H
FX3
H
H
H
H
H
NMe₂

I-450
NH
H
H
H
FX3
H
H
H
H
NMe₂

I-451
NH
FX3
H
H
H
NHMe
H
H
H
H

I-452
NH
H
FX3
H
H
NHMe
H
H
H
H

I-453
NH
H
H
FX3
H
NHMe
H
H
H
H

I-454
NH
H
H
H
FX3
NHMe
H
H
H
H

I-455
NH
FX3
H
H
H
H
NHMe
H
H
H

I-456
NH
H
FX3
H
H
H
NHMe
H
H
H

I-457
NH
H
H
FX3
H
H
NHMe
H
H
H

I-458
NH
H
H
H
FX3
H
NHMe
H
H
H

I-459
NH
FX3
H
H
H
H
H
NHMe
H
H

I-460
NH
H
FX3
H
H
H
H
NHMe
H
H

I-461
NH
H
H
FX3
H
H
H
NHMe
H
H

I-462
NH
H
H
H
FX3
H
H
NHMe
H
H

I-463
NH
FX3
H
H
H
H
H
H
NHMe
H

I-464
NH
H
FX3
H
H
H
H
H
NHMe
H

I-465
NH
H
H
FX3
H
H
H
H
NHMe
H

I-466
NH
H
H
H
FX3
H
H
H
NHMe
H

I-467
NH
FX3
H
H
H
H
H
H
H
NHMe

I-468
NH
H
FX3
H
H
H
H
H
H
NHMe

TABLE 8

I-469
NH
H
H
FX3
H
H
H
H
H
NHMe

I-470
NH
H
H
H
FX3
H
H
H
H
NHMe

I-471
O
H
H
HOX
H
H
H
H
H
H

I-472
O
H
H
HOX
H
H
H
NH₂
H
H

I-473
O
H
H
HOX
H
H
H
OMe
H
H

I-474
O
H
H
HOX
H
H
H
OH
H
H

I-475
O
H
H
HOX2
H
H
H
H
H
H

I-476
O
H
H
HOX2
H
H
H
NH₂
H
H

I-477
O
H
H
HOX2
H
H
H
OMe
H
H

I-478
O
H
H
HOX2
H
H
H
OH
H
H

I-479
O
H
H
HOX3
H
H
H
H
H
H

I-480
O
H
H
HOX3
H
H
H
NH₂
H
H

I-481
O
H
H
HOX3
H
H
H
OMe
H
H

I-482
O
H
H
HOX3
H
H
H
OH
H
H

I-483
O
H
H
H
H
H
H
HOX
H
H

I-484
O
H
H
H
H
H
H
HOX2
H
H

I-485
O
H
H
H
H
H
H
HOX3
H
H

I-486
O
HOX
H
H
H
NMe₂
H
H
H
H

I-487
O
H
HOX
H
H
NMe₂
H
H
H
H

I-488
O
H
H
HOX
H
NMe₂
H
H
H
H

I-489
O
H
H
H
HOX
NMe₂
H
H
H
H

I-490
O
HOX
H
H
H
H
NMe₂
H
H
H

I-491
O
H
HOX
H
H
H
NMe₂
H
H
H

I-492
O
H
H
HOX
H
H
NMe₂
H
H
H

I-493
O
H
H
H
HOX
H
NMe₂
H
H
H

I-494
O
HOX
H
H
H
H
H
NMe₂
H
H

I-495
O
H
HOX
H
H
H
H
NMe₂
H
H

I-496
O
H
H
HOX
H
H
H
NMe₂
H
H

I-497
O
H
H
H
HOX
H
H
NMe₂
H
H

I-498
O
HOX
H
H
H
H
H
H
NMe₂
H

I-499
O
H
HOX
H
H
H
H
H
NMe₂
H

I-500
O
H
H
HOX
H
H
H
H
NMe₂
H

I-501
O
H
H
H
HOX
H
H
H
NMe₂
H

I-502
O
HOX
H
H
H
H
H
H
H
NMe₂

I-503
O
H
HOX
H
H
H
H
H
H
NMe₂

I-504
O
H
H
HOX
H
H
H
H
H
NMe₂

I-505
O
H
H
H
HOX
H
H
H
H
NMe₂

I-506
O
HOX
H
H
H
NHMe
H
H
H
H

I-507
O
H
HOX
H
H
NHMe
H
H
H
H

I-508
O
H
H
HOX
H
NHMe
H
H
H
H

I-509
O
H
H
H
HOX
NHMe
H
H
H
H

I-510
O
HOX
H
H
H
H
NHMe
H
H
H

I-511
O
H
HOX
H
H
H
NHMe
H
H
H

I-512
O
H
H
HOX
H
H
NHMe
H
H
H

I-513
O
H
H
H
HOX
H
NHMe
H
H
H

I-514
O
HOX
H
H
H
H
H
NHMe
H
H

I-515
O
H
HOX
H
H
H
H
NHMe
H
H

I-516
O
H
H
HOX
H
H
H
NHMe
H
H

I-517
O
H
H
H
HOX
H
H
NHMe
H
H

I-518
O
HOX
H
H
H
H
H
H
NHMe
H

I-519
O
H
HOX
H
H
H
H
H
NHMe
H

I-520
O
H
H
HOX
H
H
H
H
NHMe
H

I-521
O
H
H
H
HOX
H
H
H
NHMe
H

I-522
O
HOX
H
H
H
H
H
H
H
NHMe

I-523
O
H
HOX
H
H
H
H
H
H
NHNe

I-524
O
H
H
HOX
H
H
H
H
H
NHMe

I-525
O
H
H
H
HOX
H
H
H
H
NHMe

I-526
O
HOX2
H
H
H
NMe₂
H
H
H
H

I-527
O
H
HOX2
H
H
NMe₂
H
H
H
H

I-528
O
H
H
HOX2
H
NMe₂
H
H
H
H

I-529
O
H
H
H
HOX2
NMe₂
H
H
H
H

I-530
O
HOX2
H
H
H
H
NMe₂
H
H
H

I-531
O
H
HOX2
H
H
H
NMe₂
H
H
H

I-532
O
H
H
HOX2
H
H
NMe₂
H
H
H

I-533
O
H
H
H
HOX2
H
NMe₂
H
H
H

I-534
O
HOX2
H
H
H
H
H
NMe₂
H
H

I-535
O
H
HOX2
H
H
H
H
NMe₂
H
H

TABLE 9

I-536
O
H
H
HOX2
H
H
H
NMe₂
H
H

I-537
O
H
H
H
HOX2
H
H
NMe₂
H
H

I-538
O
HOX2
H
H
H
H
H
H
NMe₂
H

I-539
O
H
HOX2
H
H
H
H
H
NMe₂
H

I-540
O
H
H
HOX2
H
H
H
H
NMe₂
H

I-541
O
H
H
H
HOX2
H
H
H
NMe₂
H

I-542
O
HOX2
H
H
H
H
H
H
H
NMe₂

I-543
O
H
HOX2
H
H
H
H
H
H
NMe₂

I-544
O
H
H
HOX2
H
H
H
H
H
NMe₂

I-545
O
H
H
H
HOX2
H
H
H
H
NMe₂

I-546
O
HOX2
H
H
H
NHMe
H
H
H
H

I-547
O
H
HOX2
H
H
NHMe
H
H
H
H

I-548
O
H
H
HOX2
H
NHMe
H
H
H
H

I-549
O
H
H
H
HOX2
NHMe
H
H
H
H

I-550
O
HOX2
H
H
H
H
NHMe
H
H
H

I-551
O
H
HOX2
H
H
H
NHMe
H
H
H

I-552
O
H
H
HOX2
H
H
NHMe
H
H
H

I-553
O
H
H
H
HOX2
H
NHMe
H
H
H

I-554
O
HOX2
H
H
H
H
H
NHMe
H
H

I-555
O
H
HOX2
H
H
H
H
NHMe
H
H

I-556
O
H
H
HOX2
H
H
H
NHMe
H
H

I-557
O
H
H
H
HOX2
H
H
NHMe
H
H

I-558
O
HOX2
H
H
H
H
H
H
NHMe
H

I-559
O
H
HOX2
H
H
H
H
H
NHMe
H

I-560
O
H
H
HOX2
H
H
H
H
NHMe
H

I-561
O
H
H
H
HOX2
H
H
H
NHMe
H

I-562
O
HOX2
H
H
H
H
H
H
H
NHMe

I-563
O
H
HOX2
H
H
H
H
H
H
NHMe

I-564
O
H
H
HOX2
H
H
H
H
H
NHMe

I-565
O
H
H
H
HOX2
H
H
H
H
NHMe

I-566
O
HOX3
H
H
H
NMe₂
H
H
H
H

I-567
O
H
HOX3
H
H
NMe₂
H
H
H
H

I-568
O
H
H
HOX3
H
NMe₂
H
H
H
H

I-569
O
H
H
H
HOX3
NMe₂
H
H
H
H

I-570
O
HOX3
H
H
H
H
NMe₂
H
H
H

I-571
O
H
HOX3
H
H
H
NMe₂
H
H
H

I-572
O
H
H
HOX3
H
H
NMe₂
H
H
H

I-573
O
H
H
H
HOX3
H
NMe₂
H
H
H

I-574
O
HOX3
H
H
H
H
H
NMe₂
H
H

I-575
O
H
HOX3
H
H
H
H
NMe₂
H
H

I-576
O
H
H
HOX3
H
H
H
NMe₂
H
H

I-577
O
H
H
H
HOX3
H
H
NMe₂
H
H

I-578
O
HOX3
H
H
H
H
H
H
NMe₂
H

I-579
O
H
HOX3
H
H
H
H
H
NMe₂
H

I-580
O
H
H
HOX3
H
H
H
H
NMe₂
H

I-581
O
H
H
H
HOX3
H
H
H
NMe₂
H

I-582
O
HOX3
H
H
H
H
H
H
H
NMe₂

I-583
O
H
HOX3
H
H
H
H
H
H
NMe₂

I-584
O
H
H
HOX3
H
H
H
H
H
NMe₂

I-585
O
H
H
H
HOX3
H
H
H
H
NMe₂

I-586
O
HOX3
H
H
H
NHMe
H
H
H
H

I-587
O
H
HOX3
H
H
NHMe
H
H
H
H

I-588
O
H
H
HOX3
H
NHMe
H
H
H
H

I-589
O
H
H
H
HOX3
NHMe
H
H
H
H

I-590
O
HOX3
H
H
H
H
NHMe
H
H
H

I-591
O
H
HOX3
H
H
H
NHMe
H
H
H

I-592
O
H
H
HOX3
H
H
NHMe
H
H
H

I-593
O
H
H
H
HOX3
H
NHMe
H
H
H

I-594
O
HOX3
H
H
H
H
H
NHMe
H
H

I-595
O
H
HOX3
H
H
H
H
NHMe
H
H

I-596
O
H
H
HOX3
H
H
H
NHMe
H
H

I-597
O
H
H
H
HOX3
H
H
NHMe
H
H

I-598
O
HOX3
H
H
H
H
H
H
NHMe
H

I-599
O
H
HOX3
H
H
H
H
H
NHMe
H

I-600
O
H
H
HOX3
H
H
H
H
NHMe
H

I-601
O
H
H
H
HOX3
H
H
H
NHMe
H

I-602
O
HOX3
H
H
H
H
H
H
H
NHMe

TABLE 10

I-603
O
H
HOX3
H
H
H
H
H
H
NHMe

I-604
O
H
H
HOX3
H
H
H
H
H
NHMe

I-605
O
H
H
H
HOX3
H
H
H
H
NHMe

I-606
NH
H
H
HOX
H
H
H
H
H
H

I-607
NH
H
H
HOX
H
H
H
NH₂
H
H

I-608
NH
H
H
HOX
H
H
H
OMe
H
H

I-609
NH
H
H
HOX
H
H
H
OH
H
H

I-610
NH
H
H
HOX2
H
H
H
H
H
H

I-611
NH
H
H
HOX2
H
H
H
NH₂
H
H

I-612
NH
H
H
HOX2
H
H
H
OMe
H
H

I-613
NH
H
H
HOX2
H
H
H
OH
H
H

I-614
NH
H
H
HOX3
H
H
H
H
H
H

I-615
NH
H
H
HOX3
H
H
H
NH₂
H
H

I-616
NH
H
H
HOX3
H
H
H
OMe
H
H

I-617
NH
H
H
HOX3
H
H
H
OH
H
H

I-618
NH
H
H
H
H
H
H
HOX
H
H

I-619
NH
H
H
H
H
H
H
HOX2
H
H

I-620
NH
H
H
H
H
H
H
HOX3
H
H

I-621
NH
HOX
H
H
H
NMe₂
H
H
H
H

I-622
NH
H
HOX
H
H
NMe₂
H
H
H
H

I-623
NH
H
H
HOX
H
NMe₂
H
H
H
H

I-624
NH
H
H
H
HOX
NMe₂
H
H
H
H

I-625
NH
HOX
H
H
H
H
NMe₂
H
H
H

I-626
NH
H
HOX
H
H
H
NMe₂
H
H
H

I-627
NH
H
H
HOX
H
H
NMe₂
H
H
H

I-628
NH
H
H
H
HOX
H
NMe₂
H
H
H

I-629
NH
HOX
H
H
H
H
H
NMe₂
H
H

I-630
NH
H
HOX
H
H
H
H
NMe₂
H
H

I-631
NH
H
H
HOX
H
H
H
NMe₂
H
H

I-632
NH
H
H
H
HOX
H
H
NMe₂
H
H

I-633
NH
HOX
H
H
H
H
H
H
NMe₂
H

I-634
NH
H
HOX
H
H
H
H
H
NMe₂
H

I-635
NH
H
H
HOX
H
H
H
H
NMe₂
H

I-636
NH
H
H
H
HOX
H
H
H
NMe₂
H

I-637
NH
HOX
H
H
H
H
H
H
H
NMe₂

I-638
NH
H
HOX
H
H
H
H
H
H
NMe₂

I-639
NH
H
H
HOX
H
H
H
H
H
NMe₂

I-640
NH
H
H
H
HOX
H
H
H
H
NMe₂

I-641
NH
HOX
H
H
H
NHMe
H
H
H
H

I-642
NH
H
HOX
H
H
NHMe
H
H
H
H

I-643
NH
H
H
HOX
H
NHMe
H
H
H
H

I-644
NH
H
H
H
HOX
NHMe
H
H
H
H

I-645
NH
HOX
H
H
H
H
NHMe
H
H
H

I-646
NH
H
HOX
H
H
H
NHMe
H
H
H

I-647
NH
H
H
HOX
H
H
NHMe
H
H
H

I-648
NH
H
H
H
HOX
H
NHMe
H
H
H

I-649
NH
HOX
H
H
H
H
H
NHMe
H
H

I-650
NH
H
HOX
H
H
H
H
NHMe
H
H

I-651
NH
H
H
HOX
H
H
H
NHMe
H
H

I-652
NH
H
H
H
HOX
H
H
NHMe
H
H

I-653
NH
HOX
H
H
H
H
H
H
NHMe
H

I-654
NH
H
HOX
H
H
H
H
H
NHMe
H

I-655
NH
H
H
HOX
H
H
H
H
NHMe
H

I-656
NH
H
H
H
HOX
H
H
H
NHMe
H

I-657
NH
HOX
H
H
H
H
H
H
H
NHMe

I-658
NH
H
HOX
H
H
H
H
H
H
NHMe

I-659
NH
H
H
HOX
H
H
H
H
H
NHMe

I-660
NH
H
H
H
HOX
H
H
H
H
NHMe

I-661
NH
HOX2
H
H
H
NMe₂
H
H
H
H

I-662
NH
H
HOX2
H
H
NMe₂
H
H
H
H

I-663
NH
H
H
HOX2
H
NMe₂
H
H
H
H

I-664
NH
H
H
H
HOX2
NMe₂
H
H
H
H

I-665
NH
HOX2
H
H
H
H
NMe₂
H
H
H

I-666
NH
H
HOX2
H
H
H
NMe₂
H
H
H

I-667
NH
H
H
HOX2
H
H
NMe₂
H
H
H

I-668
NH
H
H
H
HOX2
H
NMe₂
H
H
H

I-669
NH
HOX2
H
H
H
H
H
NMe₂
H
H

TABLE 11

I-670
NH
H
HOX2
H
H
H
H
NMe₂
H
H

I-671
NH
H
H
HOX2
H
H
H
NMe₂
H
H

I-672
NH
H
H
H
HOX2
H
H
NMe₂
H
H

I-673
NH
HOX2
H
H
H
H
H
H
NMe₂
H

I-674
NH
H
HOX2
H
H
H
H
H
NMe₂
H

I-675
NH
H
H
HOX2
H
H
H
H
NMe₂
H

I-676
NH
H
H
H
HOX2
H
H
H
NMe₂
H

I-677
NH
HOX2
H
H
H
H
H
H
H
NMe₂

I-678
NH
H
HOX2
H
H
H
H
H
H
NMe₂

I-679
NH
H
H
HOX2
H
H
H
H
H
NMe₂

I-680
NH
H
H
H
HOX2
H
H
H
H
NMe₂

I-681
NH
HOX2
H
H
H
NHMe
H
H
H
H

I-682
NH
H
HOX2
H
H
NHMe
H
H
H
H

I-683
NH
H
H
HOX2
H
NHMe
H
H
H
H

I-684
NH
H
H
H
HOX2
NHMe
H
H
H
H

I-685
NH
HOX2
H
H
H
H
NHMe
H
H
H

I-686
NH
H
HOX2
H
H
H
NHMe
H
H
H

I-687
NH
H
H
HOX2
H
H
NHMe
H
H
H

I-688
NH
H
H
H
HOX2
H
NHMe
H
H
H

I-689
NH
HOX2
H
H
H
H
H
NHMe
H
H

I-690
NH
H
HOX2
H
H
H
H
NHMe
H
H

I-691
NH
H
H
HOX2
H
H
H
NHMe
H
H

I-692
NH
H
H
H
HOX2
H
H
NHMe
H
H

I-693
NH
HOX2
H
H
H
H
H
H
NHMe
H

I-694
NH
H
HOX2
H
H
H
H
H
NHMe
H

I-695
NH
H
H
HOX2
H
H
H
H
NHMe
H

I-696
NH
H
H
H
HOX2
H
H
H
NHMe
H

I-697
NH
HOX2
H
H
H
H
H
H
H
NHMe

I-698
NH
H
HOX2
H
H
H
H
H
H
NHMe

I-699
NH
H
H
HOX2
H
H
H
H
H
NHMe

I-700
NH
H
H
H
HOX2
H
H
H
H
NHMe

I-701
NH
HOX3
H
H
H
NMe₂
H
H
H
H

I-702
NH
H
HOX3
H
H
NMe₂
H
H
H
H

I-703
NH
H
H
HOX3
H
NMe₂
H
H
H
H

I-704
NH
H
H
H
HOX3
NMe₂
H
H
H
H

I-705
NH
HOX3
H
H
H
H
NMe₂
H
H
H

I-706
NH
H
HOX3
H
H
H
NMe₂
H
H
H

I-707
NH
H
H
HOX3
H
H
NMe₂
H
H
H

I-708
NH
H
H
H
HOX3
H
NMe₂
H
H
H

I-709
NH
HOX3
H
H
H
H
H
NMe₂
H
H

I-710
NH
H
HOX3
H
H
H
H
NMe₂
H
H

I-711
NH
H
H
HOX3
H
H
H
NMe₂
H
H

I-712
NH
H
H
H
HOX3
H
H
NMe₂
H
H

I-713
NH
HOX3
H
H
H
H
H
H
NMe₂
H

I-714
NH
H
HOX3
H
H
H
H
H
NMe₂
H

I-715
NH
H
H
HOX3
H
H
H
H
NMe₂
H

I-716
NH
H
H
H
HOX3
H
H
H
NMe₂
H

I-717
NH
HOX3
H
H
H
H
H
H
H
NMe₂

I-718
NH
H
HOX3
H
H
H
H
H
H
NMe₂

I-719
NH
H
H
HOX3
H
H
H
H
H
NMe₂

I-720
NH
H
H
H
HOX3
H
H
H
H
NMe₂

I-721
NH
HOX3
H
H
H
NHMe
H
H
H
H

I-722
NH
H
HOX3
H
H
NHMe
H
H
H
H

I-723
NH
H
H
HOX3
H
NHMe
H
H
H
H

I-724
NH
H
H
H
HOX3
NHMe
H
H
H
H

I-725
NH
HOX3
H
H
H
H
NHMe
H
H
H

I-726
NH
H
HOX3
H
H
H
NHMe
H
H
H

I-727
NH
H
H
HOX3
H
H
NHMe
H
H
H

I-728
NH
H
H
H
HOX3
H
NHMe
H
H
H

I-729
NH
HOX3
H
H
H
H
H
NHMe
H
H

I-730
NH
H
HOX3
H
H
H
H
NHMe
H
H

I-731
NH
H
H
HOX3
H
H
H
NHMe
H
H

I-732
NH
H
H
H
HOX3
H
H
NHMe
H
H

I-733
NH
HOX3
H
H
H
H
H
H
NHMe
H

I-734
NH
H
HOX3
H
H
H
H
H
NHMe
H

I-735
NH
H
H
HOX3
H
H
H
H
NHMe
H

I-736
NH
H
H
H
HOX3
H
H
H
NHMe
H

TABLE 12

I-737
NH
HOX3
H
H
H
H
H
H
H
NHMe

I-738
NH
H
HOX3
H
H
H
H
H
H
NHMe

I-739
NH
H
H
HOX3
H
H
H
H
H
NHMe

I-740
NH
H
H
H
HOX3
H
H
H
H
NHMe

I-741
O
I
H
H
H
H
H
HOX
H
H

I-742
O
I
H
H
H
H
H
HOX2
H
H

I-743
O
I
H
H
H
H
H
HOX3
H
H

I-744
O
I
H
H
H
H
H
FX
H
H

I-745
O
I
H
H
H
H
H
FX2
H
H

I-746
O
I
H
H
H
H
H
FX3
H
H

I-747
NH
I
H
H
H
H
H
HOX
H
H

I-748
NH
I
H
H
H
H
H
HOX2
H
H

I-749
NH
I
H
H
H
H
H
HOX3
H
H

I-750
NH
I
H
H
H
H
H
FX
H
H

I-751
NH
I
H
H
H
H
H
FX2
H
H

I-752
NH
I
H
H
H
H
H
FX3
H
H

Among the above-described compounds, I-6 (compound 14), I-7 (compound 17), I-8 (compound 20), I-86 (compound 7), I-106 (compound 12), I-741 (compound 26), I-742 (compound 27) and I-743 (compound 28) are preferred. Among them, I-86 and I-106 are more preferable.

The compound represented by general formula (I) may be synthesized according to the descriptions in the Examples provided later, Varma R S et al, Tetrahedron Letters, 33(40), 5937-40, 1992, and the like.

The compound represented by general formula (I) is preferably labeled with a labeling substance. As the labeling substance, fluorescent substances, affinity-substances, and the like may be used. Preferably, radionuclides are used. Types of radionuclides used for labeling are not particularly limited, and may be suitably selected depending on the embodiment of use. For example, when the compound represented by general formula (I) is used for diagnosis by single photon emission computed tomography (SPECT), γ-ray-emitting radionuclides such as ^99mTc, ¹¹¹In, ⁶⁷Ga, ²⁰¹TI, ¹²³I, and ¹³³Xe (preferably, ^99mTc and ¹²³I) may be used as the radionuclides. Furthermore, when the compound represented by the general formula (I) is used for diagnosis by positron emission tomography (PET), positron-emitting radionuclides such as ¹¹C, ¹³N, ¹⁵O, ¹⁸F, ⁶²Cu, ⁶⁸Ga, and ⁷⁶Br (preferably, ¹¹C, ¹³N, ¹⁵O, and ¹⁸F) may be used. Furthermore, when the compound represented by general formula (I) is administered to animals other than human, radionuclides having a longer half life, such as ¹²⁵I, may be used. Radionuclides may be contained in the molecule of the compound represented by general formula (I) or bound to the compound represented by general formula (I).

As the method for binding a radionuclide to the compound represented by general formula (I), a method commonly used for each radionuclide may be used. Furthermore, when a radionuclide is bound to the compound represented by general formula (I), the radionuclide alone may be bound. Alternatively, a radionuclide bound to another substance may be bound. Since the above-mentioned ^99mTc is usually bound to a compound to be labeled in the form of a complex, a complex containing ^99mTc may be bound to the compound represented by general formula (I). Examples of complexes containing ^99mTc include a complex containing 2-hydrazinopyridine (Liu S et al., Bioconjug Chem. 1996 January-February; 7(1): 63-71), a complex containing N-(2-mercaptoethyl)-2-[(2-mercaptoethyl)amino]-acetamide (Zhen W et al., J Med. Chem. 1999 Jul. 29; 42(15): 2805-15), a complex containing 2,2′-(1,2-ethanediyldiimino)bis-ethanethiol (Oya S et al., Nucl Med. Biol. 1998 February; 25(2): 135-40), a tricarbonyl complex (Schibli R et al., Bioconjug Chem. 2000 May-June; 11(3): 345-51), and so forth.

Instead of the compound represented by general formula (I), a pharmaceutically acceptable salt thereof may be used. Examples of pharmaceutically acceptable salts include alkali metal salts (sodium salts, potassium salts, and lithium salts), alkaline earth metal salts (calcium salts and magnesium salts), sulfates, hydrochlorides, nitrates, phosphates, and so forth.

The composition of the present invention is used for diagnosing an amyloid-related disease. Here, the term “amyloid-related disease” means a disease caused by accumulation of amyloid β protein, primarily Alzheimer's disease; this term also includes diseases such as Down's syndrome, hereditary cerebral hemorrhage with amyloidosis-Dutch type (HCHWA-D), etc. Furthermore, prodromes of such diseases, which are not generally recognized as a “disease,” are also included in the “amyloid-related disease” in the present invention. As an examples of such a prodrome, mild cognitive impairment (MC1) observed before the onset of Alzheimer's disease may be given.

Usually, diagnosis of an amyloid-related disease using the composition of the present invention is made by administering the composition of the present invention to a patient for whom a diagnosis is to be made, a laboratory animal, or the like, then imaging the brain thereof, and making diagnosis based on the state of the compound represented by general formula (I) (quantity, distribution, etc.) in the image. The administration method of the composition of the present invention is not particularly limited and may be suitably selected depending on the type of a compound, the type of a labeling substance, and the like. Usually, the composition of the present invention is administered by injection, drip infusion, or the like into the skin, the peritoneal cavity, the vein, the artery, or the spinal fluid. The dose of the composition of the present invention is not particularly limited and may be suitably selected depending on the type of a compound, the type of a labeling substance, and the like. For adults, preferably 10⁻¹⁰to 10⁻³mg, more preferably 10⁻⁸to 10⁻⁵mg of the compound represented by general formula (I) per day is administered.

Since the composition of the present invention is usually administered by injection or drip infusion as described above, components usually contained in injection solutions or drip infusion solutions may be contained in the composition. Examples of such components include liquid carriers (for example, potassium phosphate buffer, physiological saline, Ringer's solution, distilled water, polyethylene glycol, vegetable oils and fats, ethanol, glycerine, dimethyl sulfoxide, propylene glycol, etc.), antibacterial agents, local anesthetics (for example, procaine hydrochloride, dibucaine hydrochloride, etc.), buffers (for example, Tris-hydrochloride buffer, HEPES buffer, etc.), and osmotic modifiers (for example, glucose, sorbitol, sodium chloride, etc.).

The composition for diagnosing amyloid-related diseases of the present invention may also be used for screening for therapeutic or prophylactic agents for amyloid-related diseases. For example, a test substance is administered to a model animal of a “disease” such as Alzheimer's disease; the composition for diagnosing amyloid-related diseases of the present invention is administered to the model animal; and then the distribution or the quantity of the compound represented by general formula (I) contained in the brain of the model animal is examined. As a result, when a significant difference (for example, a reduced distribution site, a decreased quantity, etc.) is detected as compared with a control (model animal not receiving the test substance), the test substance can be a candidate therapeutic agent for the amyloid-related disease. Furthermore, after a test substance is administered to a model animal of a “prodrome of a disease” such as mild cognitive impairment, the composition for diagnosing amyloid-related diseases of the present invention is administered to the model animal, and then the distribution or the quantity of the compound represented by general formula (I) contained in the brain of the model animal is examined. As a result, when a significant difference (for example, a reduction or a slowed enlargement of distribution site, a decrease or a slowed increase in quantity, etc.) is detected as compared with a control, the test substance can be a candidate prophylactic agent for the amyloid-related disease.

Furthermore, the composition for diagnosing amyloid-related diseases of the present invention may also be used to evaluate therapeutic or prophylactic agents for amyloid-related diseases whose effects have already been confirmed. Specifically, after the therapeutic or prophylactic agent for the disease is administered to a model animal of an amyloid-related disease, the composition for diagnosing amyloid-related diseases of the present invention is administered to the model animal, and then the distribution or the quantity of the compound represented by general formula (I) contained in the brain of the model animal is examined. This allows evaluation of the above-mentioned therapeutic or prophylactic agent (specifically, effective dosage, effective administration method, etc.).

EXAMPLES

Hereafter, the present invention will be explained more specifically with reference to the following examples.

Example 1
Experimental Methods
(1) Reagents and Instruments

As radioactive iodine-125 (¹²⁵I), IODINE-125 (185 MBq) from Amersham Bioscience was used. For reversed-phase HPLC, Cosmosil 5C18-AR column (4.6×150 mm) from Nacalai Tesque was used with an elution solvent of ultrapure water (A) and acetonitrile (B) mixed at A:B=30:70, at a flow rate of 1.0 mL/min. Mass spectra were obtained on a JEOL IMS-DX instrument. Amyloid (3 protein (Human, 1-42) [TFA form] was purchased from Peptide Institute, Inc. Other reagents were of special grade. ¹H-NMR spectra were obtained on a Varian Gemini 300 spectrometer with tetramethylsilane as an internal standard.

(2) Synthesis of Aurone Derivatives
Synthesis of 2-(2-(methoxy-2-oxyethoxy)-5-bromobenzoic acid methyl ester (Compound 1)

To a solution of 2-hydroxy-5-bromobenzoic acid methyl ester (1.5 g, 6.49 mmol) in acetone (10 mL) was added K₂CO₃(2.7 g). Subsequently, ethylbromoacetate (1.3 mL) was added thereto dropwise under stirring, and the mixture was heated to reflux for 3 h. After completion of the reaction was completed, the solvent was evaporated. The residue was dissolved in purified water (100 mL) and extracted with ethyl acetate (100 mL). After the extract was dried over anhydrous sodium sulfate, evaporation of the solvent gave Compound 1. Yield: 1.60 g (yield rate: 77.7%). ¹H NMR NMR (300 MHz, CDCl₃) d 1.29 (t, J=7.2 Hz, 3H), 3.91 (s, 3H), 4.24 (q, J=7.2 Hz, 2H), 4.69 (s, 2H), 6.78 (d, J=9.0 Hz, 1H), 7.54 (d, J=6.3 Hz, 1H), 7.96 (s, 1H).

Synthesis of 2-(carboxymethoxy)-5-bromobenzoic acid (Compound 2)

To a solution of Compound 1 (1.6 g, 5.04 mmol) in methanol (10 mL) was added 10% NaOH (3.0 mL). The mixture was reacted for 2 h. After completion of the reaction, 1 N HCl was added under cooling. The resultant crystals were filtered to give Compound 2. Yield: 1.10 g (yield rate: 79.4%).

Synthesis of 5-bromo-3-acetoxybenzofuran (Compound 3)

In a mixture of acetic anhydride (20 mL) and acetic acid (4 mL), Compound 2 (1.10 g, 4.00 mmol) was dissolved. After addition of sodium acetate (1.0 g) thereto, the mixture was heated to reflux for 5 h. After completion of the reaction, purified water (100 mL) was added, and the reaction mixture was extracted with chloroform (100 mL) to give Compound 3. Yield: 0.70 g (yield rate: 68.4%) ¹H NMR (300 MHz, CDCl₃) d 2.37 (s, 3H), 7.33 (d, J=9.0 Hz, 1H), 7.43 (dd, J=3.6, 2.1 Hz, 1H), 4.69 (s, 2H), 7.71 (s, 1H), 8.02 (s, 1H).

Synthesis of 5-bromo-3(2H)-benzofuranone (Compound 4)

A mixture of Compound 3, methanol (10 mL), purified water (5 mL), and 1 N HCl (2 mL) was heated to reflux for 3 h under stirring. After completion of the reaction, the reaction solution was cooled and purified water (100 ml) was added thereto. The crystals formed were collected by vacuum filtration to give Compound 4. Yield: 0.50 g (yield rate: 86.9%) ¹H NMR (300 MHz, CDCl₃) d 4.67 (s, 2H), 7.06 (d, J=9.0 Hz, 1H), 7.69 (dd, J=2.1, 2.1 Hz, 1H), 7.79 (d, J=2.1 Hz, 1H).

Synthesis of 5-bromo-4′-dimethylaminoaurone (Compound 5)

To a solution of Compound 4 (50 mg, 0.23 mmol) and 4-dimethylaminobenzaldehyde (35 mg, 0.235 mmol) in chloroform (5 mL) was added aluminum oxide (1.6 g). The mixture was stirred for 20 min. After completion of the reaction, the reaction mixture was extracted with chloroform (100 mL). Evaporation of the solvent gave Compound 5. Yield: 42 mg (yield rate: 51.9%) ¹H NMR (300 MHz, CDCl₃) d 3.09 (s, 6H), 6.70 (d, J=11.4 Hz, 2H), 6.95 (s, 1H), 7.22 (d, J=8.7 Hz, 1H), 7.67 (d, J=9.6 Hz, 1H), 7.83 (d, J=9.0 Hz, 2H), 7.92 (d, J=2.1 Hz, 1H).

Synthesis of 5-tributyltin-4′-dimethylaminoaurone (Compound 6)

To a solution of Compound 5 (12 mg, 0.035 mmol) in dioxane (5 mL), bis(tributyltin) (0.3 mL), tetra-triphenylphosphinepalladium (50 mg, 0.043 mmol) and triethylamine (5 mL) were added. The mixture was heated at 90° C. for 3 h. After the solvent was evaporated, the residue was subjected to silica gel column chromatography with an elution solvent of ethyl acetate/hexane (1/4) to thereby obtain Compound 6. Yield: 7.0 mg (yield rate: 35.2%) ¹H NMR (300 MHz, CDCl₃) d 0.86-1.57 (m, 27H), 3.07 (s, 6H), 6.75 (d, J=9.0 Hz, 2H), 6.92 (s, 1H), 7.29 (d, J=7.2 Hz, 1H), 7.67 (dd, J=1.2, 0.9 Hz, 1H), 7.86 (d, J=9.0 Hz, 2H), 7.95 (s, 1H).

Synthesis of 5-iodo-4′-dimethylaminoaurone (Compound 7)

To a solution of Compound 6 (7 mg, 0.35 mmol) in chloroform (3 mL), iodine in chloroform (0.7 mL, 0.25 M) was added at room temperature under stirring. After 10 min reaction at room temperature, saturated aqueous solution of sodium hydrogen sulfite (15 mL) was added to terminate the reaction. The chloroform layer was separated and dried with sodium sulfate. After evaporation of the solvent, the residue was subjected to silica gel chromatography using ethyl acetate/hexane (1/4) as an elution solvent to thereby obtain 7. Yield: 3.0 mg (yield rate: 63.9%) ¹H NMR (300 MHz, CDCl₃) d 3.08 (s, 6H), 6.74 (d, J=9.0 Hz, 2H), 6.95 (s, 1H), 7.12 (d, J=8.7 Hz, 1H), 7.82-7.88 (m, 3H), 8.12 (s, 1H). MS m/z 391 (M⁺).

Synthesis of 5-bromo-4′-nitroaurone (Compound 8)

To a solution of Compound 4 (100 mg, 0.47 mmol) and 4-nitrobenzaldehyde (80 mg, 0.53 mmol) in chloroform, aluminum oxide (3.0 g) was added and the mixture was stirred for 20 min. After completion of the reaction, the reaction mixture was extracted with chloroform. Then, evaporation of the solvent gave Compound 8. Yield: 100 mg (yield rate: 61.6%).

Synthesis of 5-bromo-4′-aminoaurone (Compound 9)

To a solution of Compound 8 (10 mg, 0.028 mmol) in ethanol (10 mL), tin(II) chloride (0.64 g, 2.37 mmol) was added gradually under stirring. The mixture was heated to reflux for 2 h. After completion of the reaction, 1 N aqueous sodium hydroxide (100 mL) was added. Then, the reaction mixture was extracted with ethyl acetate (100 mL) and dried over anhydrous sodium acetate. Evaporation of the solvent gave Compound 9. Yield: 7.0 mg (yield rate: 79.1%) ¹H NMR (300 MHz, CDCl₃) d 4.11 (s, 2H), 6.72 (d, J=8.7 Hz, 2H), 6.90 (s, 1H), 7.23 (d, J=8.4 Hz, 1H), 7.70 (dd, J=2.1, 2.1 Hz, 1H), 7.76 (d, J=8.4 Hz, 2H), 7.92 (d, J=1.8 Hz, 1H).

Synthesis of 5-bromo-4′-methylaminoaurone (Compound 10)

Compound 9 (100 mg, 0.316 mmol) was dissolved in DMSO (7 mL).

Subsequently, K₂CO₃(380 mg) and CH₃I (0.15 mL) were added thereto and the mixture was stirred at 50° C. for 3 h. After completion of the reaction, purified water (100 mL) was added. The reaction mixture was extracted with ethyl acetate (100 mL). The solvent was evaporated and the residue was subjected to silica gel column chromatography using ethyl acetate/hexane (1/2) as an elution solvent, to thereby obtain Compound 10. Yield: 49 mg (yield rate: 47.5%) ¹H NMR (300 MHz, CDCl₃) d 2.92 (s, 3H), 4.21 (s, 1H), 6.59 (d, J=8.7 Hz, 2H), 6.92 (s, 1H), 7.25 (d, J=8.7 Hz, 1H), 7.68 (dd, J=2.1, 2.1 Hz, 1H), 7.78 (d, J=8.7 Hz, 2H), 7.91 (d, J=2.1 Hz, 1H).

Synthesis of 5-tributyltin-4′-methylaminoaurone (Compound 11)

To a solution of Compound 10 (49 mg, 0.15 mmol) in dioxane (5 mL), bis(tributyltin) (0.4 mL), tetra-triphenylphosphinepalladium (50 mg, 0.043 mmol) and triethylamine (5 mL) were added. The mixture was heated at 90° C. for 5 h. After evaporation of the solvent, the residue was subjected to silica gel column chromatography using ethyl acetate/hexane (1/2) as an elution solvent, to thereby obtain Compound 11. Yield: 28 mg (yield rate: 34.6%) ¹H NMR (300 MHz, CDCl₃) d 1.06-1.57 (m, 27H), 3.12 (s, 3H), 4.42 (s, 1H), 6.73 (d, J=8.7 Hz, 2H), 7.10 (s, 1H), 7.49 (d, J=8.1 Hz, 1H), 7.88 (dd, J=1.2, 1.2 Hz, 1H), 8.01 (d, J=8.7 Hz, 2H), 8.09 (s, 1H).

Synthesis of 5-iodo-4′-methylaminoaurone (Compound 12)

To a solution of Compound 11 (30 mg, 0.055 mmol) in chloroform (3 mL), iodine in chloroform (1 mL, 0.25 M) was added at room temperature under stirring. After 10 min reaction at room temperature, saturated aqueous solution of sodium hydrogen sulfite (15 mL) was added to terminate the reaction. The chloroform layer was separated and dried with sodium sulfate. After evaporation of the solvent, the residue was subjected to silica gel column chromatography using ethyl acetate/hexane (1/2) as an elution solvent to thereby obtain 12. Yield: 10 mg (yield rate: 48.2%) ¹H NMR (300 MHz, CDCl₃) d 2.92 (s, 3H), 4.27 (s, 1H), 6.64 (d, J=9.0 Hz, 2H), 6.90 (s, 1H), 7.12 (d, J=8.4 Hz, 1H), 7.79 (d, J=8.7 Hz, 2H), 7.86 (dd, J=2.1, 2.1 Hz, 1H), 8.11 (d, J=2.1 Hz, 1H).

Synthesis of 5-tributyltin-4′-aminoaurone (Compound 13)

To a solution of Compound 9 (78 mg, 0.25 mmol) in dioxane (7 mL), bis(tributyltin) (0.4 mL, tetra-triphenylphosphinepalladium (50 mg, 0.043 mmol) and triethylamine (5 mL) were added. The mixture was heated at 90° C. for 4 h. After evaporation of the solvent, the residue was subjected to silica gel column chromatography using ethyl acetate/hexane (1/2) as an elution solvent to thereby obtain 13. Yield: 30 mg (yield rate: 23.1%) ¹H NMR (300 MHz, CDCl₃) d 0.86-1.57 (m, 27H), 4.05 (s, 2H), 6.73 (d, J=8.7 Hz, 2H), 6.87 (s, 1H), 7.30 (d, J=8.1 Hz, 1H), 7.69 (dd, J=0.6, 0.9 Hz, 1H), 7.78 (d, J=8.4 Hz, 2H), 7.89 (s, 1H).

Synthesis of 5-iodo-4′-aminoauron (Compound 14)

To a solution of Compound 13 (30 mg, 0.057 mmol) in chloroform (3 mL), iodine in chloroform (1 mL, 0.25 M) was added at room temperature. After 10 min reaction at room temperature, saturated aqueous solution of sodium hydrogen sulfite (15 mL) was added to terminate the reaction. The chloroform layer was separated and dried with sodium sulfate. After evaporation of the solvent, the residue was subjected to silica gel column chromatography using ethyl acetate/hexane (1/2) as an elution solvent to thereby obtain 14. Yield: 14 mg (yield rate: 67.6%) ¹H NMR (300 MHz, CDCl₃) d 4.10 (s, 2H), 6.72 (d, J=8.7 Hz, 2H), 6.90 (s, 1H), 7.13 (d, J=9.0 Hz, 1H), 7.76 (d, J=8.4 Hz, 2H), 7.87 (d, J=10.5 Hz, 1H), 8.11 (s, 1H).

Synthesis of 5-bromo-4′-methoxyaurone (Compound 15)

To a solution of Compound 4 (300 mg, 1.41 nmol) and 4-methoxybenzaldehyde (192 mg, 1.41 mmol) in chloroform, aluminum oxide (5.0 g) was added, and the mixture was stirred for 20 min. After completion of the reaction, the reaction mixture was extracted with chloroform (100 mL). Evaporation of the solvent gave Compound 15. Yield: 410 mg (yield rate: 85.7%) ¹H NMR (300 MHz, CDCl₃) d 3.88 (s, 3H), 6.91 (s, 1H), 6.99 (d, J=9.0 Hz, 2H), 7.24 (d, J=8.4 Hz, 1H), 7.73 (dd, J=2.1, 2.1 Hz, 1H), 7.88 (d, J=8.7 Hz, 2H), 7.92 (d, J=1.8 Hz, 1H).

Synthesis of 5-tributyltin-4′-methoxyaurone (Compound 16)

To a solution of Compound 9 (200 mg, 0.60 mmol) in dioxane (5 mL), bis(tributyltin) (0.4 mL), tetra-triphenylphosphinepalladium (50 mg, 0.043 mmol) and triethylamine (5 mL) were added. The mixture was heated at 90° C. for 24 h. After evaporation of the solvent, the residue was subjected to silica gel column chromatography using chloroform as an elution solvent to thereby obtain 16. Yield: 50 mg (yield rate: 15.3%) ¹H NMR (300 MHz, CDCl₃) δ 0.89-1.64 (m, 27H), 3.87 (s, 3H), 6.89 (s, 1H), 6.99 (d, J=8.7 Hz, 2H), 7.31 (d, J=8.1 Hz, 1H), 7.71 (dd, J=0.9, 0.9 Hz, 1H), 7.86 (d, J=9.0 Hz, 2H), 7.90 (d, J=8.7 Hz 1H).

Synthesis of 5-iodo-4′-methoxyaurone (Compound 17)

To a solution of Compound 16 (10 mg, 0.35 mmol) in chloroform (3 mL), iodine in chloroform (1 mL, 0.25 M) was added at room temperature. After 10 min reaction at room temperature, saturated aqueous solution of sodium hydrogen sulfite (15 mL) was added to terminate the reaction. The chloroform layer was separated and dried with sodium sulfate. After evaporation of the solvent, the residue was subjected to silica gel column chromatography using chloroform as an elution solvent to thereby obtain 17. Yield: 7 mg (yield rate: 71.5%) ¹H NMR (300 MHz, CDCl₃) d 3.88 (s, 3H), 6.91 (s, 1H), 6.98 (d, J=6.9 Hz, 2H), 7.14 (d, J=8.4 Hz, 1H), 7.87-7.91 (m, 3H), 8.12 (d, J=2.1 Hz, 1H).

Synthesis of 5-bromo-4′-hydroxyaurone (Compound 18)

Compound 15 (300 mg, 0.91 mmol) was dissolved in dichloromethane (25 mL).

Under ice-cooling, boron tribromide in dichloromethane (3.0 mL) was gradually added thereto. After reaction on ice, the reaction mixture was gradually added to ice water and extracted separately in dichloromethane layer and aqueous layer. After evaporation of the solvent, the residue was subjected to silica gel column chromatography using ethyl acetate/hexane (3/2) as an elution solvent to thereby obtain 18. Yield: 15 mg (yield rate: 5.2%).

Synthesis of 5-tributyltin-4′-hydroxyaurone (Compound 19)

To a solution of Compound 18 (50 mg, 0.16 mmol) in dioxane (5 mL), bis(tributyltin) (0.4 mL), tetra-triphenylphosphinepalladium (50 mg, 0.043 mmol) and triethylamine (5 mL) were added. The mixture was heated at 90° C. for 10 h. After evaporation of the solvent, the residue was subjected to silica gel column chromatography using ethyl acetate/hexane (3/2) as an elution solvent to thereby obtain 19. Yield: 10 mg (yield rate: 12.0%) ¹H NMR (300 MHz, CDCl₃) d 0.86-1.60 (m, 27H), 6.74 (d, J=9.0 Hz, 2H), 6.90 (s, 1H), 7.30 (d, J=8.1 Hz, 1H), 7.69 (d, J=8.1 Hz, 1H), 7.80 (d, J=8.4 Hz, 2H), 7.89 (s, 1H).

Synthesis of 5-iodo-4′-hydroxyaurone (Compound 20)

To a solution of Compound 19 (10 mg, 0.027 mmol) in chloroform (3 mL), iodine in chloroform (1 mL, 0.25 M) was added at room temperature. After 10 min reaction at room temperature, saturated aqueous solution of sodium hydrogen sulfite (15 mL) was added to terminate the reaction. The chloroform layer was separated and dried with sodium sulfate. After evaporation of the solvent, the residue was subjected to silica gel column chromatography using ethyl acetate/hexane (3/2) as an elution solvent to thereby obtain Compound 20. Yield: 6 mg (yield rate: 84.0%).

(3) In Vitro Binding Experiment Using A3 (1-42) Aggregates

Aβ aggregates were prepared by dissolving in a buffer containing 10 mM sodium phosphate and 1 mM EDTA (pH 7.4) at a concentration of 0.5 mg/mL and incubating at 37° C. for 42 h. The binding experiment was performed using 12×75 mm borosilicate glass tubes. [¹²⁵I]IMPY used for comparison was prepared according to the method described previously (Zhuang Z P, Kung M P, Wilson A, Lee C W, Plossi K, Hou C, Holzman D M, Kung H F. Structure-activity relationship of imidazo[1,2-a]pyridines as ligands for detecting β-amyloid plaques in the brain. J. Med. Chem. 2003, 46(2): 237-43). 900 μL of 10% ethanol solution, 50 μL of [¹²⁵I]-labeled aurone derivative with various concentrations (in 10% aqueous ethanol) or 50 μL of [¹²⁵I]IMPY, and 50 μL (29 nM) of an Aβ(1-42) aggregate solution were mixed, and the mixture was allowed to stand at room temperature for 3 h. Aβ3 aggregate-bound aurone derivative and nonbound aurone derivative were separated with a Brandel M-24R cell harvester using Whatman GF/B filters. The radioactivities of substances remaining on the filter used for filtration were measured with a γ counter.

(4) In Vivo Radioactivity Biodistribution Experiment in Normal Mice

¹²⁵I-labeled compounds were diluted with 10% ethanol-containing physiological saline. Five 5-week-old male ddY mice per group were decapitated at 2, 10, 30, or 60 min after intravenous administration of 100 μL (0.5 to 1 μCi) of the labeled compound. After their blood was collected, organs were removed, and the weights and radioactivities thereof were measured.

(5) Autoradiography Using Brain Tissue Sections from Alzheimer's Disease Patients

Brain tissue sections from Alzheimer's disease patients (hippocampus, paraffin sections, 5 μm) were purchased from BioChain. These sections were dewaxed by washing in xylene (5 min, twice) and in 100% ethanol, 100% ethanol, 95% ethanol, 85% ethanol and 70% ethanol (each 1 min, once). Autoradiography was carried out by reacting the section with ¹²⁵I-labeled aurone compound (5-iodo-4′-aminoaurone; Compound 14) (0.2 nM) prepared in advance. After the reaction, each section was washed in a solution of saturated lithium carbonate in 40% aqueous ethanol (2 min, twice), in 40% ethanol (2 min, once) and in water (30 sec, once). After air drying, the section was fixed on an imaging plate (Fuji Film) for 48 h, followed by analysis with BAS500 (Fuji Film). Immunostaining of senile plaque amyloid was performed using sections adjacent to the brain section used in autoradiography and according to the technique described in Amyloid β Immunostaining Kit (Wako Purechemical).

[Experimental Results]
(1) Synthesis of Aurone Derivatives

FIGS. 1, 2 and 3 show synthetic pathways for aurone derivatives. The aurone skeleton was formed by aldol condensation reaction of benzofuranone (synthesized by a conventional method) and aldehyde. Compounds 5, 9, 10, 15 and 18 were converted to tributyltin forms through a reaction with bis(tributyltin) using palladium as a catalyst. Compounds 6, 11, 13, 16 and 19 in tributyltin forms were converted to Compounds 7, 12, 14, 17 and 20 through a reaction with iodine. Further, the tributyltin compounds were labeled with ¹²⁵I through a conventional tributyltin-iodine exchange reaction. Briefly, 50 μL of a tributyltin compound (1.0 mg/ml ethanol solution) was placed in a glass vial to which 1-2 μL of [¹²⁵I]NaI (3700-7400 kBq), 50 μL of 1 N HCl and 50 μL of 3% hydrogen peroxide were added. The resultant mixture was left at room temperature for 1 to 3 min. Then, 100 μL of saturated aqueous sodium hydrogen sulfite and 100 μL of saturated aqueous sodium hydrogen carbonate were added thereto to terminate the reaction. After addition of ethyl acetate (1 mL), the reaction mixture was fed to a Pasteur pipette packed with sodium sulfate for dehydration. The ethyl acetate was evaporated under nitrogen gas flow. The residue was dissolved in 100 μl of ethanol and purified by reversed-phase HPLC using water:acetonitrile (3:7) as an elution solvent, to thereby obtain a ¹²⁵I-labelled aurone derivative of interest.

(2) Binding Experiment of Aurone Derivatives to Ab (1-42) Aggregates

FIGS. 4 and 5 show the results of experiments in which aurone derivatives were reacted in the presence and absence of Ab aggregates; the reaction mixture was filtered with a cell harvester; and the remaining radioactivity on the filter was measured. While the remaining radioactivity on the filter was low in any aurone derivatives in the absence of Ab aggregates, ¹²⁵I-labeled aurone derivatives showed remarkably high radioactivities in the presence of Ab aggregates (FIG. 5). These results revealed that any aurone derivative possesses a high binding property to Ab aggregates, though the property varies depending of the type of the substituent introduced into their side chains. The intensity of the binding property increased in the following order: [¹²⁵I] Compound 7>[¹²⁵I] Compound 12=[¹²⁵I] IMPY>[¹²⁵I] Compound 14=[¹²⁵I] Compound 17>[¹²⁵I] Compound 20 (FIG. 4). The binding property to Ab aggregates of those compounds with —NH₂, OMe- and OH group in their side chains are lower than the binding property of [¹²⁵] IMPY. On the other hand, those compounds with NHMe- and NMe₂group show equal or higher binding property to Ab aggregates compared to the binding property of [¹²⁵I] IMPY. All the 5 types of aurone derivatives tested this time showed a very high binding property to Ab aggregates. Among all, the binding of Compound 7 to Ab aggregates was equal or more than the binding of [¹²⁵I] IMPY which is clinically tested at present. Therefore, it has become clear that this compound satisfies a critical requirement as an imaging probe for amyloid β.

(3) In Vivo Radioactivity Distribution Experiment in Mice

FIG. 13 shows biodistribution of radioactivity in normal mice after the injection of aurone derivatives. When any of the aurone derivatives was used, sufficient radioactivity to perform imaging of intracerebral amyloid migrated to the brain from the early stage of injection (1.7-4.6% ID/g). Subsequently, rapid clearance of radioactivity was observed with the passage of time. The radioactivity remaining in the brain at 30 min after injection was 7-12% of the radioactivity remaining at 2 min after injection; and the radioactivity remaining in the brain at 60 min after injection was 2-8% of the radioactivity remaining at 2 min after injection. These results suggested that when aurone derivatives are applied to Alzheimer's disease model mice or Alzheimer's disease patients, they should exhibit a high binding property to amyloid deposited sites and rapid clearance of radioactivity from normal sites in the brain suffering from Alzheimer's disease; thus, aurone derivatives are capable of achieving a high S/N ratio. It was also shown that, in any of the aurone derivatives, in vivo radioactivity behavior is discharged mainly from the liver to the bile duct and the intestinal tract.

TABLE 13

Biodistribution of Radioactivity in Normal Mice^a

Time (min) after Injection

Tissue
2
10
30
60

[¹²⁵I]7

Blood
2.69 (0.29)
1.96 (0.34)
1.48 (0.15)
0.94 (0.25)

Liver
10.21 (1.87)
7.86 (2.25)
5.60 (0.59)
4.33 (0.76)

Kidney
7.84 (1.92)
8.31 (3.19)
10.37 (2.34)
4.51 (1.43)

Intestine
1.65 (0.35)
5.37 (1.10)
9.50 (1.45)
11.85 (2.70)

Spleen
1.76 (0.28)
0.81 (0.54)
0.51 (0.10)
0.37 (0.11)

Lung
4.02 (0.83)
2.44 (0.54)
1.51 (0.41)
1.17 (0.26)

Stomach^b
1.21 (0.29)
1.35 (1.25)
1.87 (0.79)
1.51 (1.04)

Pancreas
3.17 (0.68)
1.04 (0.25)
0.55 (0.21)
0.30 (0.06)

Heart
3.80 (0.66)
1.36 (0.66)
0.55 (0.29)
0.45 (0.11)

Brain
1.89 (0.38)
0.69 (0.21)
0.26 (0.04)
0.11 (0.03)

[¹²⁵I]12

Blood
4.16 (0.98)
2.72 (1.01)
2.84 (1.48)
2.41 (0.70)

Liver
11.26 (2.81)
9.70 (2.78)
6.11 (2.16)
4.91 (0.72)

Kidney
9.91 (2.00)
11.44 (2.76)
6.00 (2.14)
3.52 (0.77)

Intestine
3.06 (1.11)
6.59 (3.43)
10.34 (4.91)
14.04 (2.87)

Spleen
2.37 (0.41)
1.28 (0.19)
0.86 (0.14)
0.75 (0.17)

Lung
6.86 (0.65)
4.44 (0.39)
2.41 (0.66)
2.94 (0.62)

Stomach^b
1.30 (0.43)
2.53 (0.97)
2.82 (1.88)
2.26 (0.88)

Pancreas
4.83 (0.60)
1.81 (0.15)
0.83 (0.10)
0.77 (0.28)

Heart
5.07 (0.47)
2.17 (0.34)
0.98 (0.21)
0.84 (0.37)

Brain
3.17 (0.45)
1.22 (0.09)
0.32 (0.02)
0.24 (0.05)

[¹²⁵I]14

Blood
3.64 (0.78)
4.38 (0.52)
2.87 (0.82)
2.35 (1.38)

Liver
9.17 (3.45)
8.48 (2.84)
7.64 (2.19)
5.85 (1.09)

Kidney
10.87 (2.95)
13.12 (4.30)
12.05 (5.44)
4.95 (1.82)

Intestine
3.12 (0.85)
5.06 (1.51)
15.47 (5.48)
18.30 (7.21)

Spleen
2.29 (0.36)
1.64 (0.29)
0.84 (0.29)
0.92 (0.07)

Lung
9.06 (0.47)
7.01 (0.28)
5.83 (0.55)
5.23 (1.44)

Stomach^b
1.59 (0.71)
2.94 (0.86)
4.01 (2.29)
3.49 (2.00)

Pancreas
4.96 (0.44)
2.01 (0.49)
1.22 (0.21)
0.87 (0.30)

Heart
6.77 (0.74)
2.69 (0.45)
1.30 (0.41)
0.98 (0.25)

Brain
4.57 (0.27)
1.51 (0.17)
0.49 (0.06)
0.26 (0.03)

[¹²⁵I]17

Blood
3.16 (0.82)
1.51 (0.23)
0.95 (0.19)
0.70 (0.60)

Liver
6.87 (2.18)
5.16 (0.73)
2.76 (0.40)
1.86 (0.83)

Kidney
7.26 (2.18)
7.00 (1.49)
4.93 (1.52)
2.43 (1.17)

Intestine
1.59 (0.51)
4.70 (1.46)
11.67 (3.56)
9.02 (3.14)

Spleen
1.45 (0.56)
0.74 (0.10)
0.48 (0.10)
0.43 (0.14)

Lung
6.71 (1.39)
1.76 (0.29)
0.97 (0.20)
0.75 (0.33)

Stomach^b
0.68 (0.33)
1.06 (0.66)
0.48 (0.09)
0.96 (0.46)

Pancreas
2.83 (0.75)
0.77 (0.17)
0.29 (0.14)
0.16 (0.05)

Heart
3.84 (1.04)
1.06 (0.10)
0.37 (0.09)
0.25 (0.12)

Brain
1.69 (0.43)
0.54 (0.12)
0.11 (0.05)
0.03 (0.02)

[¹²⁵I]20

Blood
3.14 (0.73)
2.77 (0.28)
1.75 (0.38)
0.87 (0.28)

Liver
6.05 (1.49)
6.63 (1.08)
4.23 (0.63)
4.45 (3.14)

Kidney
11.08 (2.96)
11.22 (2.26)
5.82 (1.11)
2.43 (1.04)

Intestine
2.11 (0.75)
6.12 (0.83)
12.67 (2.13)
14.87 (5.42)

Spleen
2.18 (0.48)
1.36 (0.15)
0.69 (0.14)
0.42 (0.02)

Lung
6.69 (0.86)
3.14 (0.23)
1.62 (0.22)
0.80 (0.19)

Stomach^b
1.30 (0.43)
2.53 (0.97)
2.82 (1.88)
2.26 (0.88)

Pancreas
5.28 (0.99)
2.65 (0.64)
0.92 (0.19)
0.36 (0.09)

Heart
6.23 (0.63)
2.57 (0.41)
0.98 (0.14)
0.42 (0.09)

Brain
3.07 (0.39)
1.48 (0.19)
0.37 (0.07)
0.14 (0.12)

^aExpressed as % injected dose per gram, Each value represents the mean for 3-5 animals.

Values in parentheses are standard deviations.

^bExpressed as % injected dose per organ.

(4) Autoradiography Using Brain Tissue Sections from Alzheimer's Disease Patients

FIG. 6 shows the results of autoradiography and immunostaining using hippocampal sections from Alzheimer's disease patients. In this Figure, panel A shows the results of autoradiography with aurone derivatives. Panels B, C, D and E show the results of immunostaining with anti-Aβ342 antibody. In the site E where accumulation of radioactivity was not observed, deposition of senile plaques by immunostaining was not recognized. On the other hand, in the sites B, C and D where intensive radioactivity was observed in autoradiography, deposition of senile plaques by immunostaining was recognized. These results demonstrated that aurone derivatives have a selective binding property to senile plaques on brain sections from Alzheimer's disease patients.

Example 2
Experimental Methods
(1) Reagents and Instruments

The same reagents and instruments as used in Example 1 were used.

(2) Synthesis of Aurone Derivatives
Synthesis of 2-((ethoxycarbonyl)methoxy)-5-iodobenzoic acid methyl ester (Compound 21)

To a solution of 2-hydroxy-5-iodobenzoic acid methyl ester (2.0 g, 7.19 mmol) in acetone (10 mL), K₂CO₃(2.7 g) was added. Subsequently, ethylbromoacetate (2.0 mL) was added thereto dropwise under stirring. The mixture was heated to reflux for 3 h. After completion of the reaction, the solvent was evaporated. The residue was dissolved in purified water (100 mL) and extracted with ethyl acetate (100 mL). The extract was dried over anhydrous sodium sulfate, and evaporation of the solvent gave Compound 21. Yield: 2.62 g (yield rate: 99%) ¹H NMR (300 MHz, CDCl₃) 1.29 (t, J=7.2 Hz, 3H), 3.90 (s, 3H), 4.25 (q, J=6.0 Hz, 2H), 4.69 (s, 2H), 6.66 (d, J=8.7 Hz, 1H), 7.71 (dd, J=2.4, 2.4 Hz, 1H), 8.12 (d, J=2.1 Hz, 1H).

Synthesis of 2-(carboxymethoxy)-5-iodobenzoic acid (Compound 22)

To a solution of Compound 21 (2.62 g, 7.19 mmol) in methanol (2 mL), 10% KOH (3.0 mL) was gradually added thereto. The mixture was reacted for 2 h. After completion of the reaction, 1 N HCl was added under cooling. The formed crystals were vacuum filtered to give Compound 22. Yield: 2.02 g (yield rate: 87.2%).

Synthesis of 5-iodobenzofuran-3-ylacetate (Compound 23)

Compound 22 (2.02 g, 6.27 mmol) was dissolved in a mixture of acetic anhydride (30 mL) and acetic acid (6 mL). Then, sodium acetate (1.5 g) was added thereto, and the mixture was heated to reflux for 24 h. After completion of the reaction, the reaction mixture was dissolved in 100 ml of purified water and extracted with 200 mL of ethyl acetate, to thereby obtain Compound 23. Yield: 1.45 g (yield rate: 76.6%) ¹H NMR (300 MHz, CDCl₃). 2.37 (s, 3H), 7.23 (d, J=8.1 Hz, 1H), 7.59 (d, J=1.5, 1.8 Hz, 1H), 7.90 (d, J=1.5 Hz, 1H), 7.98 (s, 1H).

Synthesis of 5-iodobenzofuran-3(2H)-one (Compound 24)

To a solution of Compound 23 (1.45 g, 4.80 mmol) in methanol (18 mL), purified water (10 mL) and 1N HCl (4 mL) were added. The mixture was heated to reflux for 3 h under stirring. After completion of the reaction, the reaction mixture was cooled and purified water (100 mL) was added thereto. The resultant crystals were vacuum filtered to thereby obtain Compound 24. Yield: 1.17 g (yield rate: 93.8%) ¹H NMR (300 MHz, CDCl₃). 4.65 (s, 2H), 6.96 (d, J=9.0 Hz, 1H), 7.85 (dd, J=1.8, 1.8 Hz, 1H), 7.99 (d, J=1.8 Hz, 1H).

Synthesis of (Z)-2-(4-hydroxybenzylidene)-5-iodobenzofuran-3(2H)-one (Compound 25)

To a solution of Compound 24 (50 mg, 0.1922 mmol) and 4-hydroxybenzaldehyde (30 mg, 0.246 mmol) in chloroform, aluminum (1.7 g) was added. Then, the mixture was stirred for 10 h. After completion of the reaction, the reaction mixture was extracted with methanol (100 mL). Evaporation of the solvent gave Compound 25. Yield: 78 mg (yield rate: 87.0%) ¹H NMR (300 MHz, CDCl₃)-6.91 (s, 1H), 6.99 (d, J=6.9 Hz, 2H), 7.14 (d, J=8.4 Hz, 1H), 7.85-7.90 (m, 3H), 8.11 (d, J=2.1 Hz, 1H).

Synthesis of (Z)-2-(4-(2-hydroxyethoxy)benzylidene)-5-iodobenzofuran-3(2H)-one (Compound 26)

To a solution of Compound 25 (194 mg, 0.533 mmol) and ethylene chlorohydrin (0.1 mL, 1.43 mmol) in dimethylsulfoxide (7 mL), potassium carbonate (3.7 g) was added.

The mixture was heated and stirred for 15 h. After completion of the reaction, the reaction mixture was extracted with ethyl acetate (100 mL). After the solvent was evaporated, the residue was subjected to silica gel column chromatography using ethyl acetate as an elution solvent, to thereby obtain Compound 26. Yield: 123 mg (yield rate: 56.5%) ¹H NMR (300 MHz, CDCl₃). 2.01 (s, 1H), 4.02 (s, 2H), 4.18 (t, J=3.9 Hz, 2H), 6.89 (s, 1H), 7.00 (d, J=7.5 Hz, 2H), 7.14 (d, J=8.4 Hz, 1H), 7.80-7.92 (m, 3H), 8.12 (d, J=1.8 Hz, 1H).

Synthesis of (Z)-2-(4-(2-(2-hydroxyethoxy)ethoxy)benzylidene)-5-iodobenzofuran-3(2H)-one (Compound 27)

To a solution of Compound 25 (150 mg, 0.412 mmol) and ethylene glycol mono-2-chloroethyl ether (0.15 mL, 1.42 mmol) in dimethylsulfoxide (7 mL), potassium carbonate (3.7 g) was added. The mixture was heated and stirred for 5 h. After completion of the reaction, the reaction mixture was extracted with ethyl acetate (200 mL). After the solvent was evaporated, the residue was subjected to silica gel column chromatography using ethyl acetate as an elution solvent, to thereby obtain Compound 27. Yield: 56 mg (yield rate: 30.2%) ¹H NMR (300 MHz, CDCl₃). 2.17 (s, 1H), 3.70 (d, J=4.8 Hz, 2H), 3.78 (d, J=4.8 Hz, 2H), 3.92 (t, J=4.8 Hz, 2H), 4.21 (t, J=4.5 Hz, 2H), 6.89 (s, 1H), 7.00 (d, J=11.4 Hz, 2H), 7.13 (d, J=8.7 Hz, 1H), 7.84-7.91 (m, 3H), 8.11 (d, J=2.1 Hz, 1H).

Synthesis of (Z)-2-(4-(2-(2-(2-hydroxyethoxy)ethoxy)ethoxy)benzylidene)-5-iodobenzofuran-3(2H)-one (Compound 28)

To a solution of Compound 25 (150 mg, 0.412 mmol) and 2-[2-(2-cloroethoxy)ethoxy]ethanol (0.1 mL, 0.69 mmol) in dimethylsulfoxide (7 mL), potassium carbonate (3.7 g) was added. The mixture was heated and stirred for 6 h. After completion of the reaction, the reaction mixture was extracted with ethyl acetate (200 mL).

The solvent was evaporated and the residue was subjected to silica gel column chromatography using ethyl acetate as an elution solvent, to thereby obtain Compound 28. Yield: 140 mg (yield rate: 67.9%).

Synthesis of (Z)-2-(4-(2-hydroxyethoxy)benzylidene)-5-(tributyltin)benzofuran-3(2H)-one (Compound 29)

To a solution of Compound 26 (20 mg, 0.048 mmol) in dioxane (3 mL), bis(tributyltin) (0.4 mL), tetra-triphenylphosphinepalladium (50 mg, 0.043 mmol) and triethylamine (3 mL) were added. The mixture was heated at 90° C. for 5 h. After the solvent was evaporated, the residue was subjected to silica gel column chromatography with an elution solvent of ethyl acetate/hexane (1/1) to thereby obtain Compound 29. Yield: 7.0 mg (yield rate: 25.0%) ¹H NMR (300 MHz, CDCl₃) 0.86-1.61 (m, 27H), 2.06 (s, 1H), 4.02 (s, 2H), 4.16 (t, J=3.6 Hz, 21-1H), 6.88 (s, 1H), 6.99 (d, J=9.0 Hz, 2H), 7.30 (d, J=12.3 Hz, 1H), 7.73 (d, J=9.0 Hz, 1H), 7.90-7.92 (m, 3H).

Synthesis of (Z)-2-(4-(2-(2-hydroxyethoxy)ethoxy)benzylidene)-5-(tributyltin)benzofuran-3(2H)-one (Compound 30)

To a solution of Compound 27 (40 mg, 0.089 mmol) in dioxane (7 mL), bis(tributyltin) (0.4 mL), tetra-triphenylphosphinepalladium (50 mg, 0.043 mmol) and triethylamine (4 mL) were added. The mixture was heated at 90° C. for 7 h. After the solvent was evaporated, the residue was subjected to silica gel column chromatography using ethyl acetate as an elution solvent to thereby obtain Compound 30. Yield: 17 mg (yield rate: 31.4%) ¹H NMR (300 MHz, CDCl₃). 0.86-1.32 (m, 27H), 2.11 (s, 1H), 3.70 (t, J=4.8 Hz, 2H), 3.78 (t, J=4.8 Hz, 2H), 3.91 (t, J=4.5 Hz, 2H), 4.22 (t, J=5.1 Hz, 2H), 6.89 (s, 1H), 7.00 (d, J=7.2 Hz, 2H), 7.31 (d, J=8.1 Hz, 1H), 7.71 (d, J=9.0 Hz, 1H), 7.88-7.91 (m, 3H).

Synthesis of (Z)-2-(4-(2-(2-(2-hydroxyethoxy)ethoxy)ethoxy)benzylidene)-5-(tributyltin)benzofuran-3(2H)-one (Compound 31)

To a solution of Compound 28 (10 mg, 0.02 mmol) in dioxane (5 mL), bis(tributyltin) (0.3 mL), tetra-triphenylphosphinepalladium (40 mg, 0.034 mmol) and triethylamine (4 mL) were added. The mixture was heated at 90° C. for 10 h. After the solvent was evaporated, the residue was subjected to silica gel column chromatography using ethyl acetate as an elution solvent, to thereby obtain Compound 31. Yield: 4 mg (yield rate: 30.3%) ¹H NMR (300 MHz, CDCl₃) 0.86-1.59 (m, 27H), 2.11 (s, 1H), 3.63 (t, J=3.3 Hz, 2H), 3.73-3.75 (m, 4H), 3.90 (t, J=4.2 Hz, 2H), 4.21 (t, J=4.2 Hz, 2H), 6.88 (s, 1H), 7.10 (d, J=8.7 Hz, 2H), 7.31 (d, J=8.1 Hz, 1H), 7.64-7.70 (m, 3H), 7.90 (d, J=4.8 Hz, 1H).

(3) Labeling of Aurone Derivatives with ¹²⁵I

[¹²⁵]NaI (1 to 5 mCi) and 1 N hydrochloric acid (50 mL) were added to solutions of various tributyltin compounds (Compounds 29, 30 and 31) (1 mg/mL) in ethanol (50 mL), and 3% (w/v) aqueous hydrogen peroxide (50 mL) was added finally (FIG. 9). The mixture was allowed to stand for 1 min at room temperature, then saturated aqueous sodium hydrogen sulfite (100 mL) was added to terminate the reaction, and saturated aqueous sodium hydrogen carbonate (100 mL) was added to neutralize the reaction solution. The mixture was extracted with ethyl acetate, dehydrated by passing through a Pasteur pipette containing sodium sulfate, and then purified by reversed-phase HPLC (water:acetonitrile=3:7). Nonradioactive compounds were analyzed for absorbance at 254 nm by HPLC as standards. Then, labeled compounds having matching absorbances were separated and extracted with ethyl acetate, followed by evaporation of ethyl acetate under nitrogen gas flow.

(4) Preparation of Ab(1-42) aggregates

Ab(1-42) was dissolved in 10 mM phosphate buffer containing 1 mM EDTA (pH 7.4) to give a concentration of 0.25 mg/mL, and the mixture was incubated at 37° C. for 42 h to thereby prepare Ab(1-42) aggregates.

(5) Calculation of Inhibition Constant (K_ivalues) by Binding Inhibition Experiment Using Ab(1-42) Aggregates

850 mL of 10% EtOH solution, 50 mL of a solution of [¹²⁵I](Z)-2-(4-aminobenzylidene)-5-iodobenzofuran-3(2H)-one in 10% ethanol, and 50 mL of a sample solution at various concentrations (0 to 20 mM) were mixed. Finally, 50 mL of Ab(1-42) aggregate solution was added thereto, and the mixture was allowed to stand at room temperature for 3 h. Ab(1-42) aggregate-bound compounds and nonbound compounds were separated with a Brandel M-24R cell harvester using a Whatman GF/B filter. Radioactivities remaining on the filter were measured with a γ-counter, and inhibition curves were created using Graph Pad Prism 4.0 to calculate inhibition constants (K_ivalues).

(6) In Vivo Radioactivity Distribution Experiment in Normal Mice

¹²⁵I-labeled compounds (Compounds 26, 27 and 28) were diluted with physiological saline containing 10% ethanol. To each of 5-week-old male ddY mice (5 animals/group), 100 μL of the labeled compound (0.3 to 0.5 μCi) was injected intravenously. Animals were decapitated at 2, 10, 30 or 60 min after the injection, and the blood was collected. Then, major organs were removed, and weights and radioactivities thereof were measured.

(7) Fluorescence Staining and Immunostaining on Brain Sections from Transgenic Mice

As Alzheimer's disease model mouse, Tg2576 mice (20-month-old) were used. Brain tissues were removed and frozen in carboxymethyl cellulose (4%). Then, a series of sections 10 μm thick were prepared therefrom. The thus prepared sections were reacted with a ligand (a solution of Compound 26 and thioflavin in 50% EtOH) for 3 min. Then, the sections were washed with 50% EtOH (1 min×twice) and observed under a fluorescence microscope. Further, using adjacent sections, immunostaining was performed by conventional methods with anti-amyloid (3(1-42) antibody.

(8) In Vitro Autoradiography and Immunostaining on Brain Sections from Alzheimer's Disease Patients

Hippocampal sections (5 μm thick) from Altzheimer's disease patients were dewaxed by washing with xylene ((5 min×2) and with 100% EtOH, 100% EtOH, 95% EtOH, 85% EtOH and 70% EtOH (1 min×1 for each). After air-drying, the sections were reacted with a ligand (40% EtOH solution) for 1 h. Then, the sections were washed with saturated aqueous lithium carbonate solution in 40% EtOH (2 min×2), with 40% EtOH (2 min×2) and with water (30 sec×1), and immobilized on imaging plates for 24 h. Subsequently, the sections were analyzed with BAS2500. Further, using adjacent sections, immunostaining was performed by conventional methods with anti-amyloid β(1-42) antibody.

[Experimental Results]
(1) Synthesis of Aurone Derivatives

FIG. 7 shows synthetic pathways for aurone derivatives. The aurone skeleton was formed by an aldol condensation reaction. Individual iodinated compounds were converted to tributyltin forms through a reaction with bis(tributyltin) using palladium as a catalyst. These tributyltin compounds were used as labeling precursor compounds in the labeling with radioactive iodine.

(2) ¹²⁵I Labeling Experiment of Aurone Derivatives

FIG. 8 shows a ¹²⁵I labeling pathway for aurone derivatives. ¹²⁵I labeling was performed with hydrogen peroxide as an oxidizing agent and through tin-iodine exchange reaction, to thereby obtain ¹²⁵I-labeled compounds of interest. Absorbances at 254 nm of non-radioactive compounds were analyzed by reversed-phase HPLC in advance. By isolating and purifying those labeled compounds with matching retention time, ¹²⁵I-labeled compounds of interest were obtained with a radiochemical purity of 95% or more.

(3) Examination concerning the Binding Affinity of Aurone Derivatives to Ab(1-42) Aggregates

In order to calculate inhibition constants (K_ivalues) for various aurone derivatives synthesized, binding inhibition experiments were performed using [¹²⁵I](Z)-2-(4-aminobenzylidene)-5-iodobenzofuran-3(2H)-one as a radioactive ligand. The K_ivalues obtained by the experiments are shown in Table 14. The binding affinity of aurone derivatives to Ab(1-42) aggregates is within the range from 1.05 to 3.36 nM in K_ivalues. Thus, aurone derivatives have shown a high binding property to amyloid aggregates. No difference in binding property resulting from the length of introduced ethyleneoxy chains was observed.

TABLE 14

In vitro Binding Inhibition Experiment Using A β 42 Aggregates

Compound
Ki (nM)

26
1.05 ± 0.06

27
3.36 ± 0.29

28
2.56 ± 0.31

(4) In Vivo Radioactivity Distribution Experiment of ¹²⁵I-Labeled Aurone Derivatives in Normal Mice

In vivo radioactivity distribution experiments of ¹²⁵I-labeled aurone derivatives were performed using 5-week-old normal mice. The results of biodistribution of radioactivity after injection of [¹²⁵I] Compound 26, [¹²⁵I] Compound 27 and [¹²⁵I] Compound 28 into mice from the tail vein are shown in Table 15. All of [¹²⁵I] Compound 26, [¹²⁵I] Compound 27 and [¹²⁵I] Compound 28 have shown high migration to the brain at 2 min after the injection (2.4 to 4.5% ID/g). Further, the radioactivity remaining in the brain at 30 min after injection was as low as 0.08 to 0.16% ID/g. From these results, it has become clear that these three types of aurone derivatives show rapid clearance of radioactivity from normal brain. Besides, no remarkable radioactivity stasis in the blood was observed.

TABLE 15

Radioactivity Distribution of Aurone Derivatives in the

Brain and Blood of Normal Mice

Time (min) after Injection

2
10
30
60

[125I]26

Brain

Mean
4.51
1.49
0.24
0.09

Standard Deviation
0.25
0.28
0.03
0.04

Blood

Mean
4.97
3.88
2.38
1.24

Standard Deviation
0.96
1.09
0.85
0.35

[125I]27

Brain

Mean
3.69
1.53
0.38
0.16

Standard Deviation
0.22
0.31
0.05
0.03

Blood

Mean
3.61
5.18
1.11
0.68

Standard Deviation
0.74
2.54
0.73
0.45

[125I]28

Brain

Mean
2.81
0.84
0.18
0.08

Standard Deviation
0.36
0.12
0.02
0.02

Blood

Mean
4.75
2.70
1.13
0.55

Standard Deviation
0.79
0.23
0.29
0.05

(5) Fluorescence Staining and Immunostaining on Brain Sections from Transgenic Mice

Fluorescence staining was performed on brain sections from Alzheimer's disease model mouse (Tg2576) utilizing the fluorescence property of Compound 26 (FIG. 9). As a result, the fluorescence image of Compound 26 was consistent with the fluorescence image of thioflavin S on adjacent section and the immunostaining image of anti-amyloid antibody on adjacent section. From these results, it was demonstrated that Compound 26 possesses a binding property not only to synthetic amyloid 1342 aggregates (in vitro binding experiment) but also to senile plaques in mice.

(6) In Vitro Autoradiography and Immunostaining on Brain Sections from Alzheimer's Disease Patients

Autoradiography was performed on brain sections from Altzheimer's disease patients with [¹²⁵I]26 (FIG. 10). While the radioactivity of [¹²⁵I]26 was observed at the positive site of immunostaining with anti-amyloid antibody, no accumulation of radioactivity was observed in sites without senile plaques. From these results, it was suggested that [¹²⁵I]26 has a binding property to the senile plaque amyloid accumulating in Altzheimer's disease patients.

The present specification encompasses the contents disclosed in the specifications and/or the drawings of Japanese Patent Applications (No. 2006-328131 and No. 2007-081602) based on which the present patent application claims priority. All publications, patents and patent applications cited herein are incorporated herein by reference in their entirety.

Number	Date	Country	Kind
2006-328131	Dec 2006	JP	national
2007-081602	Mar 2007	JP	national

AURONE DERIVATIVE-CONTAINING COMPOSITION FOR DIAGNOSIS

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