Claims
- 1. A nucleic acid assay system for analyzing a sample using a reagent, comprising:
holding means for receiving said sample and said reagent; PCR reactor means for amplifying said sample; detection means for detection of PCR amplicon; transport means for selectively transporting said sample and said reagent to said holding means, said PCR reactor means, and said detection means, said transport means operatively connected to said holding means, said PCR reactor means, and said detection means; control means for selectively adding said reagent to said sample, mixing said sample and said reagent, performing PCR amplification, and detecting PCR amplicon, said control means operatively connected to said holding means, said PCR reactor means, said detection means, and said transport means; and means for decontaminating said holding means, said PCR reactor means, said detection means.
- 2. The nucleic acid assay system of claim 1, including conduits within said holding means, said PCR reactor means, said detection means, and said transport means; and wherein said means for decontaminating said holding means, said PCR reactor means, said detection means includes means for decontaminating said conduits.
- 3. The nucleic acid assay system of claim 2, wherein said conduits include tubing.
- 4. The nucleic acid assay system of claim 2, wherein said conduits include microchannels.
- 5. The nucleic acid assay system of claim 2, wherein said conduits include passages within said PCR reactor means.
- 6. The nucleic acid assay system of claim 1, wherein said holding means mixes said sample with said reagent.
- 7. The nucleic acid assay system of claim 6, wherein said reagent is a PCR reagent.
- 8. The nucleic acid assay system of claim 6, wherein said PCR reagent includes primers.
- 9. The nucleic acid assay system of claim 6, wherein said PCR reagent includes oligos.
- 10. The nucleic acid assay system of claim 6, wherein said PCR reagent includes enzymes.
- 11. The nucleic acid assay system of claim 1, wherein said PCR reactor means cycles between a relatively high temperature and a relatively low temperature to produce PCR amplification.
- 12. The nucleic acid assay system of claim 1, wherein said PCR reactor means includes a section that can be held at a relatively high temperature and a section that can be held at a relatively low temperature and said PCR reactor means cycles said sample between said section that can be held at a relatively high temperature and said section that can be held at a relatively low temperature.
- 13. The nucleic acid assay system of claim 1, wherein said PCR reactor means includes an embedded thermocouple calibration conduit.
- 14. A nucleic acid assay method for analyzing a sample using a reagent, comprising the steps of:
providing a holding means for receiving said sample and said reagent; providing a PCR reactor means for amplifying said sample; providing a detection means for detection of PCR amplicon; transporting said sample and said reagent to said holding means, said PCR reactor means, and said detection means; said transport means operatively connected to said holding means, said PCR reactor means, and said detection means; providing a decontamination means for decontaminating said holding means, said PCR reactor means, said detection means; and providing a control means for selectively mixing said sample and said reagent, performing PCR amplification, detecting PCR amplicon, and decontaminating said holding means, said PCR reactor means, and said detection means; said control means operatively connected to said holding means, said PCR reactor means, and said decontamination means.
- 15. The nucleic acid assay method of claim 14, including conduits within said holding means, said PCR reactor means, said detection means, and said transport means; and wherein said decontamination means includes means for decontaminating said conduits.
- 16. The nucleic acid assay method of claim 15, wherein said conduits include tubing.
- 17. The nucleic acid assay method of claim 15, wherein said conduits include microchannels.
- 18. The nucleic acid assay method of claim 15, wherein said conduits include passages within said PCR reactor means.
- 19. The nucleic acid assay method of claim 14, wherein said sample and said reagent are mixed within said holding means.
- 20. The nucleic acid assay method of claim 19, wherein said reagent is a PCR reagent.
- 21. The nucleic acid assay method of claim 20, wherein said PCR reagent includes primers.
- 22. The nucleic acid assay method of claim 20, wherein said PCR reagent includes oligos.
- 23. The nucleic acid assay system of claim 20, wherein said PCR reagent includes enzymes.
- 24. The nucleic acid assay system of claim 14, including the step of cycling said sample between a relatively high temperature and a relatively low temperature to produce PCR amplification.
- 25. The nucleic acid assay system of claim 14, wherein said PCR reactor means includes a section that can be held at a relatively high temperature and a section that can be held at a relatively low temperature and said PCR reactor means cycles said sample between said section that can be held at a relatively high temperature and said section that can be held at a relatively low temperature.
- 26. A nucleic acid assay method for analyzing a sample, comprising the steps of:
utilizing a holding vessel for mixing said sample with a reagent; utilizing a reactor for amplifying said sample and producing an amplified sample; utilizing a detector for detecting PCR amplicon; utilizing a fluidic system for selectively transporting said sample, said reagent, and said amplified sample relative to said holding means; decontaminating and conditioning said nucleic acid assay system; and utilizing a control for controlling the selectively adding of said reagent to said sample, mixing of said sample and said reagent, performing PCR amplification, detecting PCR amplicon, and decontaminating and conditioning said nucleic acid assay system.
- 27. The nucleic acid assay method of claim 26, wherein said reagent is a PCR reagent.
- 28. The nucleic acid assay method of claim 26, wherein said PCR reagent includes primers.
- 29. The nucleic acid assay method of claim 26, wherein said PCR reagent includes oligos.
- 30. The nucleic acid assay system of claim 26, wherein said PCR reagent includes enzymes.
- 31. The nucleic acid assay system of claim 26, including the step of cycling said sample between a relatively high temperature and a relatively low temperature to produce PCR amplification.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional Application No. 60/303,537, filed Jul. 6, 2001, and titled “AUTOMATED NUCLEIC ACID ASSAY SAMPLE PREPARATION AND DETECTION,” which is incorporated herein by this reference.
Government Interests
[0002] The United States Government has rights in this invention pursuant to Contract No. W-7405-ENG-48 between the United States Department of Energy and the University of California for the operation of Lawrence Livermore National Laboratory.
Provisional Applications (1)
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Number |
Date |
Country |
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60303637 |
Jul 2001 |
US |