AUTOMATION OF QUANTITATIVE 2-D GEL STAINING

Information

  • Research Project
  • 2649451
  • ApplicationId
    2649451
  • Core Project Number
    R43GM057754
  • Full Project Number
    1R43GM057754-01
  • Serial Number
    57754
  • FOA Number
  • Sub Project Id
  • Project Start Date
    3/15/1998 - 26 years ago
  • Project End Date
    9/14/1998 - 26 years ago
  • Program Officer Name
  • Budget Start Date
    3/15/1998 - 26 years ago
  • Budget End Date
    9/14/1998 - 26 years ago
  • Fiscal Year
    1998
  • Support Year
    1
  • Suffix
  • Award Notice Date
    3/13/1998 - 26 years ago

AUTOMATION OF QUANTITATIVE 2-D GEL STAINING

This proposal is to develop a complete integrated and automated system for silver staining and scanning two-dimensional high resolution gels to both increasing sensitivity and resolving power, and of improving quantitation. This system is required for studies on changes in gene expression in cells and tissues in response to drugs, xenobiotics, injury, stress and disease. The number of variables in the over 50 variations of silver staining published are large, making optimization using manual procedures extremely costly and tedious. In addition, quantitation is now realized to require dynamic measurements on each spot during image development since the kinetics of image development differs for different proteins. We have developed a prototype robotic silver staining system and propose its completion together with the development of a novel system for repeatedly scanning each gel during development. This requires a novel gel transport system, and a combined scanner-development system including a scanner which can require images at short intervals. The completed systems is expected to process up to 40 gels per day. The software required to match successive images, calculate integrated absorbances as a function of time, and, by reference to calibration runs, determine the amount of each protein present, will also be written. PROPOSED COMMERCIAL APPLICATION Potential commercial applications: A completely automatic computerized system for optimizing silver staining of 2D gels will be a key factor in the development of a high throughput 2D protein analysis systems essential for the development of proteomics.

IC Name
NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
  • Activity
    R43
  • Administering IC
    GM
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    821
  • Ed Inst. Type
  • Funding ICs
  • Funding Mechanism
  • Study Section
    ZRG3
  • Study Section Name
  • Organization Name
    LARGE SCALE BIOLOGY CORPORATION
  • Organization Department
  • Organization DUNS
  • Organization City
    GERMANTOWN
  • Organization State
    MD
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    208767005
  • Organization District
    UNITED STATES