Patent Application
20100004230
This invention relates to novel compounds, compositions containing them and their use as antibacterials.
WO02/08224, WO02/50061, WO02/56882, WO02/96907, WO2003087098, WO2003010138, WO2003064421, WO2003064431, WO2004002992, WO2004002490, WO2004014361, WO2004041210, WO2004096982, WO2002050036, WO2004058144, WO2004087145, WO06002047, WO06014580, WO06010040, WO06017326, WO06012396, WO06017468, WO06020561, WO01/25227, WO02/40474, WO02/07572, WO2004035569, WO2004089947, WO04024712, WO04024713, WO04087647, WO2005016916, WO2005097781, WO06010831, WO04035569, WO04089947, WO06021448, WO06032466, WO06038172, WO06046552, WO06134378 and WO06137485 disclose quinoline, naphthyridine, morpholine, cyclohexane, piperidine and piperazine derivatives having antibacterial activity.
This invention provides a compound of formula (I) or a pharmaceutically acceptable salt, solvate and/or N-oxide thereof:
wherein:
one of B and D is CH2 and the other is a bond;
one of Z1 and Z2 is CH or N and the other is CH;
R1a and R1b are independently selected from hydrogen; halogen; cyano; (C1-6)alkyl; (C1-6)alkylthio; trifluoromethyl; trifluoromethoxy; carboxy; hydroxy optionally substituted with (C1-6)alkyl or (C1-6)alkoxy-substituted(C1-6)alkyl; (C1-6)alkoxy-substituted(C1-6)alkyl; hydroxy(C1-6)alkyl; an amino group optionally N-substituted by one or two (C1-6)alkyl, formyl, (C1-6)alkylcarbonyl or (C1-6)alkylsulphonyl groups; or aminocarbonyl wherein the amino group is optionally substituted by (C1-4)alkyl;
provided that R1b is H when Z1 is N;
R2 is hydrogen, or (C1-4)alkyl, or together with R6 forms Y as defined below;
in which: R3 is as defined for R1a or R1b or is oxo and n is 1 or 2:
or A is a group (ii)
W1, W2 and W3 are CR4R8
or W2 and W3 are CR4R8 and W1 represents a bond between W3 and N.
X is O, CR4R8, or NR6;
one R4 is as defined for R1a and R1b and the remainder and R8 are hydrogen or one R4 and R8 are together oxo and the remainder are hydrogen;
R6 is hydrogen or (C1-6)alkyl; or together with R2 forms Y;
R7 is hydrogen; halogen; hydroxy optionally substituted with (C1-6)alkyl; or (C1-6)alkyl;
Y is CR4R8CH2; CH2CR4R8; (C═O); CR4R8; CR4R8(C═O); or (C═O)CR4R8;
or when X is CR4R8, R8 and R7 together represent a bond;
U is selected from CO, and CH2 and
R5 is an optionally substituted bicyclic carbocyclic or heterocyclic ring system (B):
containing up to four heteroatoms in each ring in which
at least one of rings (a) and (b) is aromatic;
X1 is C or N when part of an aromatic ring, or CR14 when part of a non-aromatic ring;
X2 is N, NR13, O, S(O)X, CO or CR14 when part of an aromatic or non-aromatic ring or may in addition be CR14R15 when part of a non aromatic ring;
X3 and X5 are independently N or C;
Y1 is a 0 to 4 atom linker group each atom of which is independently selected from N, NR13, O, S(O)X, CO and CR14 when part of an aromatic or non-aromatic ring or may additionally be CR14R15 when part of a non aromatic ring;
Y2 is a 2 to 6 atom linker group, each atom of Y2 being independently selected from N, NR13, O, S(O)X, CO, CR14 when part of an aromatic or non-aromatic ring or may additionally be CR14R15 when part of a non aromatic ring;
each of R14 and R15 is independently selected from: H; (C1-4)alkylthio; halo; carboxy(C1-4)alkyl; (C1-4)alkyl; (C1-4)alkoxycarbonyl; (C1-4)alkylcarbonyl; (C1-4)alkoxy(C1-4)alkyl; hydroxy; hydroxy(C1-4)alkyl; (C1-4)alkoxy; nitro; cyano; carboxy; amino or aminocarbonyl optionally mono- or di-substituted by (C1-4)alkyl; or
R14 and R15 may together represent oxo;
each R13 is independently H; trifluoromethyl; (C1-4)alkyl optionally substituted by hydroxy, (C1-6)alkoxy, (C1-6)alkylthio, halo or trifluoromethyl; (C2-4)alkenyl; (C1-4)alkoxycarbonyl; (C1-4)alkylcarbonyl; (C1-6)alkylsulphonyl; aminocarbonyl wherein the amino group is optionally mono or disubstituted by (C1-4)alkyl; and
each x is independently 0, 1 or 2.
This invention also provides a method of treatment of bacterial infections in mammals, particularly in man, which method comprises the administration to a mammal in need of such treatment an effective amount of a compound of formula (I), or a pharmaceutically acceptable salt, solvate and/or N-oxide thereof.
The invention also provides the use of a compound of formula (I), or a pharmaceutically acceptable salt, solvate and/or N-oxide thereof, in the manufacture of a medicament for use in the treatment of bacterial infections in mammals.
The invention also provides a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt, solvate and/or N-oxide thereof, and a pharmaceutically acceptable carrier.
In particular aspects:
(1) B is CH2 and D is a bond, Z1 is CH and Z2 is N;
(2) B is CH2 and D is a bond, Z1 is CH and Z2 is CH;
(3) B is CH2 and D is a bond, Z1 is N and Z2 is CH;
(4) B is a bond and D is CH2, Z1 is N and Z2 is CH; or
(5) B is a bond and D is CH2, Z1 is CH and Z2 is N.
In a particular aspect each R1a and R1b is independently hydrogen, (C1-4)alkoxy, (C1-4)alkylthio, (C1-4)alkyl, cyano, carboxy, hydroxymethyl or halogen such as fluoro; more particularly hydrogen, methoxy, methyl, cyano, or fluoro.
In certain embodiments only one group R1a or R1b is other than hydrogen. In particular embodiments R1a is fluoro or methoxy and R1b is hydrogen.
In a particular aspect R2 is hydrogen.
Particular examples of R3 include hydrogen; optionally substituted hydroxy; optionally substituted amino; halogen; (C1-4) alkyl; 1-hydroxy-(C1-4) alkyl; optionally substituted aminocarbonyl. More particular R3 groups are hydrogen; CONH2; 1-hydroxyalkyl e.g. CH2OH; optionally substituted hydroxy e.g. methoxy; optionally substituted amino; and halogen, in particular fluoro. Most particularly R3 is hydrogen, hydroxy or fluoro.
In a particular aspect, when A is (ia), n is 1. In a further aspect R3 is in the 3- or 4-position. In a more particular aspect, A is (ia), n is 1 and R3 is H or hydroxy in the 3-position, and more particularly is cis to the NR2 group.
In a particular aspect, when A is (ii), X is CR4R8 and R8 is H and R4 is H or OH. More particularly when R4 is OH it is trans to R7. In a further aspect W1 is a bond. In another aspect R7 is H. In an additional aspect W2 and W3 are both CH2. Where A is 3-hydroxypyrrolidin-4-ylmethyl, in a particular aspect the configuration is (3S,4S).
In certain embodiments U is CH2.
In certain embodiments R5 is an aromatic heterocyclic ring (B) having 8-11 ring atoms including 2-4 heteroatoms of which at least one is N or NR13 in which, in particular embodiments, Y2 contains 2-3 heteroatoms, one of which is S and 1-2 are N, with one N bonded to X3.
In alternative embodiments the heterocyclic ring (B) has ring (a) aromatic selected from optionally substituted benzo, pyrido, pyridazino and pyrimidino and ring (b) non aromatic and Y2 has 3-5 atoms, more particularly 4 atoms, including at least one heteroatom, with O, S, CH2 or NR13 bonded to X5 where R13 is other than hydrogen, and either NHCO bonded via N to X3, or O, S, CH2 or NH bonded to X3. In a particular aspect the ring (a) contains aromatic nitrogen, and more particularly ring (a) is pyridine, pyrazine or pyrimidine.
In certain embodiments R5 is:
in which:
→is the point of attachment;
Y3 is CH2 or O; and
R10 is independently selected from hydrogen, halogen, (C1-16)alkyl and (C1-6)alkoxy.
More particularly R10 is selected from hydrogen, chloro, methyl and methoxy.
Examples of rings (B) include optionally substituted:
(a) and (b) aromatic
1H-pyrrolo[2,3-b]-pyridin-2-yl, 1H-pyrrolo[3,2-b]-pyridin-2-yl, 3H-imidazo[4,5-b]-pyrid-2-yl, 3H-quinazolin-4-one-2-yl, benzimidazol-2-yl, benzo[1,2,3]-thiadiazol-5-yl, benzo[1,2,5]-oxadiazol-5-yl, benzofur-2-yl, benzothiazol-2-yl, benzo[b]thiophen-2-yl, benzoxazol-2-yl, chromen-4-one-3-yl, imidazo[1,2-a]pyridin-2-yl, imidazo-[1,2-a]-pyrimidin-2-yl, indol-2-yl, indol-6-yl, isoquinolin-3-yl, [1,8]-naphthyridine-3-yl, oxazolo[4,5-b]-pyridin-2-yl, quinolin-2-yl, quinolin-3-yl, quinoxalin-2-yl, naphthalen-2-yl, 1,3-dioxo-isoindol-2-yl, benzimidazol-2-yl, 1H-benzotriazol-5-yl, 1H-indol-5-yl, 3H-benzooxazol-2-one-6-yl, 3H-benzooxazol-2-thione-6-yl, 3H-benzothiazol-2-one-5-yl, 3H-quinazolin-4-one-6-yl, benzo[1,2,3]thiadiazol-6-yl, benzo[1,2,5]thiadiazol-5-yl, benzo[1,4]oxazin-2-one-3-yl, benzothiazol-5-yl, benzothiazol-6-yl, cinnolin-3-yl, imidazo[1,2-a]pyridazin-2-yl, pyrazolo[1,5-a]pyrazin-2-yl, pyrazolo[1,5-a]pyridin-2-yl, pyrazolo[1,5-a]pyrimidin-6-yl, pyrazolo[5,1-c][1,2,4]triazin-3-yl, pyrido[1,2-a]pyrimidin-4-one-2-yl, pyrido[1,2-a]pyrimidin-4-one-3-yl, quinazolin-2-yl, quinoxalin-6-yl, thiazolo[3,2-a]pyrimidin-5-one-7-yl, thiazolo[5,4-b]pyridin-2-yl, thieno[3,2-b]pyridin-6-yl, thiazolo[5,4-b]pyridin-6-yl, thiazolo[4,5-b]pyridin-5-yl, [1,2,3]thiadiazolo[5,4-b]pyridin-6-yl, 2H-isoquinolin-1-one-3-yl,
(a) is non aromatic
(2S)-2,3-dihydro-1H-indol-2-yl, (2S)-2,3-dihydro-benzo[1,4]dioxine-2-yl, 3-(R,S)-3,4-dihydro-2H-benzo[1,4]thiazin-3-yl, 3-(R)-2,3-dihydro-[1,4]dioxino[2,3-b]pyridin-3-yl, 3-(S)-2,3-dihydro-[1,4]dioxino[2,3-b]pyridin-3-yl, 2,3-dihydro-benzo[1,4]dioxan-2-yl, 3-substituted-3H-quinazolin-4-one-2-yl,
(b) is non aromatic
1,1,3-trioxo-1,2,3,4-tetrahydrol l6-benzo[1,4]thiazin-6-yl, benzo[1,3]dioxol-5-yl, 2,3-dihydro-benzo[1,4]dioxin-6-yl, 2-oxo-2,3-dihydro-benzooxazol-6-yl, 3-substituted-3H-benzooxazol-2-one-6-yl, 3-substituted-3H-benzooxazole-2-thione-6-yl, 3-substituted-3H-benzothiazol-2-one-6-yl, 4H-benzo[1,4]oxazin-3-one-6-yl (3-oxo-3,4-dihydro-2H-benzo[1,4]oxazin-6-yl), 4H-benzo[1,4]thiazin-3-one-6-yl (3-oxo-3,4-dihydro-2H-benzo[1,4]thiazin-6-yl), 4H-benzo[1,4]oxazin-3-one-7-yl, 4-oxo-2,3,4,5-tetrahydro-benzo[b][1,4]thiazepine-7-yl, 5-oxo-2,3-dihydro-5H-thiazolo[3,2-a]pyrimidin-6-yl, 1H-pyrido[2,3-b][1,4]thiazin-2-one-7-yl (2-oxo-2,3-dihydro-1H-pyrido[2,3-b]thiazin-7-yl), 2,3-dihydro-1H-pyrido[2,3-b][1,4]thiazin-7-yl, 2-oxo-2,3-dihydro-1H-pyrido[3,4-b]thiazin-7-yl, 2,3-dihydro-[1,4]dioxino[2,3-b]pyridin-6-yl, 2,3-dihydro-[1,4]dioxino[2,3-c]pyridin-7-yl, 2,3-dihydro-[1,4]dioxino[2,3-b]pyridin-7-yl, 3,4-dihydro-2H-benzo[1,4]oxazin-6-yl, 3,4-dihydro-2H-benzo[1,4]thiazin-6-yl, 3-oxo-3,4-dihydro-2H-pyrido[3,2-b][1,4]oxazin-6-yl, 3,4-dihydro-2H-pyrido[3,2-b][1,4]thiazin-6-yl, 3-oxo-3,4-dihydro-2H-pyrido[3,2-b][1,4]thiazin-6-yl, 3,4-dihydro-1H-quinolin-2-one-7-yl, 3,4-dihydro-1H-quinoxalin-2-one-7-yl, 6,7-dihydro-4H-pyrazolo[1,5-a]pyrimidin-5-one-2-yl, 1,2,3,4-tetrahydro-[1,8]naphthyridin-7-yl, 2-oxo-3,4-dihydro-1H-[1,8]naphthyridin-6-yl, 6-oxo-6,7-dihydro-5H-8-thia-1,2,5-triaza-naphthalen-3-yl(6-oxo-6,7-dihydro-5H-pyridazino[3,4-b][1,4]thiazin-3-yl), 2-oxo-2,3-dihydro-1H-pyrido[3,4-b][1,4]oxazin-7-yl, 2-oxo-2,3-dihydro-1H-pyrido[2,3-b][1,4]oxazin-7-yl, 6,7-dihydro-[1,4]dioxino[2,3-d]pyrimidin-2-yl, [1,3]oxathiolo[5,4-c]pyridin-6-yl, 3,4-dihydro-2H-pyrano[2,3-c]pyridine-6-yl, 2,3-dihydro[1,4]oxathiino[2,3-c]pyridine-7-yl, 2,3-dihydro-1-benzofuran-5-yl, 6,7-dihydro[1,4]dioxino[2,3-c]pyridazin-3-yl, 6,7-dihydro[1,4]oxathiino[2,3-c]pyridazin-3-yl, 2-substituted 1H-pyrimido[5,4-b][1,4]oxazin-7(6H)-one, 2-substituted 5,6-dihydropyrido[2,3-d]pyrimidin-7(1H)-one, 7-oxo-1,5,6,7-tetrahydro[1,8]naphthyridin-2-yl.
In some embodiments R13 is H if in ring (a) or in addition (C1-4)alkyl such as methyl or isopropyl when in ring (b). More particularly, in ring (b) R13 is H when NR13 is bonded to X3 and (C1-4)alkyl when NR13 is bonded to X5.
In further embodiments R14 and R15 are independently selected from hydrogen, halo, hydroxy, (C1-4) alkyl, (C1-4)alkoxy, nitro and cyano. More particularly R15 is hydrogen.
More particularly each R14 is selected from hydrogen, chloro, fluoro, hydroxy, methyl, methoxy, nitro and cyano. Still more particularly R14 is selected from hydrogen, fluorine or nitro.
Most particularly R14 and R15 are each H.
Particular groups R5 include:
especially
When used herein, the term “alkyl” includes groups having straight and branched chains, for instance, methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, t-butyl, pentyl and hexyl. The term ‘alkenyl’ should be interpreted accordingly.
Halo or halogen includes fluoro, chloro, bromo and iodo.
Haloalkyl moieties include 1-3 halogen atoms.
Compounds within the invention contain a heterocyclyl group and may occur in two or more tautomeric forms depending on the nature of the heterocyclyl group; all such tautomeric forms are included within the scope of the invention.
Some of the compounds of this invention may be crystallised or recrystallised from solvents such as aqueous and organic solvents. In such cases solvates may be formed. This invention includes within its scope stoichiometric solvates including hydrates as well as compounds containing variable amounts of water that may be produced by processes such as lyophilisation.
Furthermore, it will be understood that phrases such as “a compound of formula (I) or a pharmaceutically acceptable salt, solvate or N-oxide thereof” are intended to encompass the compound of formula (I), an N-oxide of formula (I), a pharmaceutically acceptable salt of the compound of formula (I), a solvate of formula (I), or any pharmaceutically acceptable combination of these. Thus by way of non-limiting example used here for illustrative purpose, “a compound of formula (I) or a pharmaceutically acceptable salt or solvate thereof” may include a pharmaceutically acceptable salt of a compound of formula (I) that is further present as a solvate.
Since the compounds of formula (I) are intended for use in pharmaceutical compositions it will readily be understood that in particular embodiments they are provided in substantially pure form, for example at least 60% pure, more suitably at least 75% pure and particularly at least 85%, especially at least 98% pure (% are on a weight for weight basis). Impure preparations of the compounds may be used for preparing the more pure forms used in the pharmaceutical compositions; these less pure preparations of the compounds should contain at least 1%, more suitably at least 5% and more particularly from 10 to 59% of a compound of the formula (I) or pharmaceutically acceptable salt, solvate and/or N-oxide thereof.
Particular compounds according to the invention include those mentioned in the examples and their pharmaceutically acceptable N-oxides, salts and solvates.
Pharmaceutically acceptable salts of the above-mentioned compounds of formula (I) include the acid addition or quaternary ammonium salts, for example their salts with mineral acids e.g. hydrochloric, hydrobromic, sulphuric nitric or phosphoric acids, or organic acids, e.g. acetic, fumaric, succinic, maleic, citric, benzoic, p-toluenesulphonic, methanesulphonic, naphthalenesulphonic acid or tartaric acids. Compounds of formula (I) may also be prepared as the N-oxide. The invention extends to all such derivatives.
Certain of the compounds of formula (I) may exist in the form of optical isomers, e.g. diastereoisomers and mixtures of isomers in all ratios, e.g. racemic mixtures. The invention includes all such forms, in particular the pure isomeric forms. For example the invention includes enantiomers and diastereoisomers at the attachment point of NR2 and R3. The different isomeric forms may be separated or resolved one from the other by conventional methods, or any given isomer may be obtained by conventional synthetic methods or by stereo specific or asymmetric syntheses. Certain compounds of formula (I) may also exist in polymorphic forms and the invention includes such polymorphic forms.
In a further aspect of the invention there is provided a process for preparing compounds of formula (I), and pharmaceutically acceptable salts, solvates and/or N-oxides thereof, which process comprises cyclising a compound of formula (IIA):
in which R21 is (C1-6)alkyl such as methyl, L2 is LCH2CH(OH)— or CH2═CH— where L is -A-N(R20)R2′ or a group convertible thereto, where R20 is UR5 or a group convertible thereto and R2′ is R2 or a group convertible thereto, wherein Z1, Z2, A, R1a, R1b, R2, U and R5 are as defined in formula (I),
to give a compound of formula (IIB):
and thereafter optionally or as necessary converting L to -A-NR2—UR5, interconverting any variable groups, and/or forming a pharmaceutically acceptable salt, solvate or N-oxide thereof.
Where L2 is LCH2CH(OH), the cyclisation reaction is effected by treatment of the compound of formula (IIA) with an activating agent such as trifluoromethanesulphonic anhydride, methanesulphonyl chloride, p-toluenesulphonyl chloride, methanesulfonic anhydride or p-toluene sulfonic anhydride and an organic base such as triethylamine or diisopropylethylamine. Mesylate or tosylate preparation takes place under standard conditions and the compound of formula (IIB) forms in situ.
Where L2 is CH2═CH— cyclisation can be effected by treatment of the olefin with m-chloroperbenzoic acid to generate the epoxide which cyclises in situ to give a compound of formula (IIB) in which L is OH. Alternatively treatment of the olefin with N-bromosuccinimide in water gives a compound of formula (IIB) in which L is Br.
The cyclisation produces a mixture of compounds of formula (IIB) where (i) B is CH2 and D is a bond, and (ii) B is a bond and D is CH2. The relative proportions of the resultant 5- and 6-membered ring products will be dependent on the particular substrates and the precise reaction conditions employed (for example the solvent or activating group). In cases where a mixture of products arise from the cyclisation step, chromatography may be used to separate the isomers.
L may be a hydroxy group which can be oxidised to the aldehyde by conventional means such as 1,1,1-tris-(acetyloxy)-1,1-dihydro-1,2-benziodooxol-3-(1H)-one for reductive alkylation with HA-N(R20)R2′ under conventional conditions (see for examples Smith, M. B.; March, J. M. Advanced Organic Chemistry, Wiley-Interscience).
Alternatively L may be bromo which can be alkylated with HA-N(R20)R2′ under conventional conditions.
Conveniently one of R20 and R2′ is an N-protecting group, such as such as t-butoxycarbonyl, benzyloxycarbonyl or 9-fluorenylmethyloxycarbonyl. This may be removed by several methods well known to those skilled in the art (for examples see “Protective Groups in Organic Synthesis, T. W. Greene and P. G. M. Wuts, Wiley-Interscience, 1999), for example conventional acid hydrolysis. The invention further provides compounds of formula (IIB) in which R20 is hydrogen.
The free amine of formula (JIB) in which R20 is hydrogen may be converted to NR2UR5 by conventional means such as amide or sulphonamide formation with an acyl derivative R5COW or R5SO2W, for compounds where U is CO or SO2 or, where U is CH2, by alkylation with an alkyl halide R5CH2-halide in the presence of base, acylation/reduction with an acyl derivative R5COW or reductive alkylation with an aldehyde R5CHO under conventional conditions (see for examples Smith, M. B.; March, J. M. Advanced Organic Chemistry, Wiley-Interscience). The appropriate reagents containing the required R5 group are known compounds or may be prepared analogously to known compounds, see for example WO02/08224, WO02/50061, WO02/56882, WO02/96907, WO2003087098, WO2003010138, WO2003064421, WO2003064431, WO2004002992, WO2004002490, WO2004014361, WO2004041210, WO2004096982, WO2002050036, WO2004058144, WO2004087145, WO2004/035569, WO2004/089947, WO2003082835, WO2002026723, WO06002047, WO06014580, WO06010040, WO06017326, WO06012396, WO06017468 and WO06020561, WO06132739, WO06134378, WO06137485 and EP0559285.
Where R5 contains an NH group, this may be protected with a suitable N-protecting group such as t-butoxycarbonyl, benzyloxycarbonyl or 9-fluorenylmethyloxycarbonyl during the coupling of the R5 derivative with the free amine of formula (IIB). The protecting group may be removed by conventional methods, such as by treatment with trifluoroacetic acid.
The compound of formula (IIA) may be prepared by the following Scheme 1:
(i) Heterocyclic bromides of type (1) can be converted to allyl derivatives (2) by reaction with allyl boron reagents (see Kotha, Sambasivarao et al, Synlett (2005) 12, 1877-1880), allyl tin reagents (see Bolm, Carsten et al, Journal of Organic Chemistry (2005) 70(6), 2346-2349), allyl magnesium Grignard reagents (see Hourdin, Marie et al, Journal of Combinatorial Chemistry (2005) 7(2), 285-297) or allyl indium reagents (see Lee, Phil, Advanced Synthesis & Catalysis (2004) 346(13-15), 1641-1645). Alternatively, the bromide can be metallated and the corresponding organometallic species (magnesium (Beinhoff, Matthias, Synthesis (2003) (1), 79-90); lithium (Lau, Stephen, Canadian Journal of Chemistry (2001) 79(11) 1541-1545); copper (Inoue, Atsushi, Journal of Organic Chemistry (2001) 66(12), 4333-4339)) may be alkylated with allyl bromide to give (2).
(ii) Oxidation of allyl derivatives (2) to diols (3) may be accomplished with standard reagent systems such as osmium tetroxide/N-methyl morpholine-N-oxide (Zheng, Tao et al, Journal of the American Chemical Society (2005) 127(19), 6946-6947). It will be appreciated that such oxidations can be performed chirally using appropriate chiral oxidising systems such as AD mix alpha or beta (see Pinard, E et al, Bioorganic & Medicinal Chemistry Letters (2001), 11(16), 2173-2176). This provides compounds of formula (IIA) where L2 is LCH2CH(OH).
(iii) The primary alcohol of diols (3) can be selectively activated towards displacement by reaction with sulphonyl halides (see Wallner, Sabine R, Organic & Biomolecular Chemistry (2005) 3(14), 2652-2656) and this selectivity may be enhanced by the use of catalytic quantities of dibutyltin oxide (see Boger, Dale, Journal of the American Chemical Society (1996) 118(9), 2301-2).
(iv) The sulphonates (4) may be cyclised to give epoxides (5) most conveniently under mildly basic conditions such as potassium carbonate in methanol (Gooding, Owen, Synthetic Communications (1995), 25(8), 1155-66) although strongly basic conditions such as butyl lithium may be used (Chong, J. Michael, Tetrahedron Letters (1994), 35(39), 7197-200). As an alternative to the epoxides, the diols may be activated as cyclic suphates (see Bonini, Carlo, Tetrahedron (2005) 61(27), 6580-6589). It will be appreciated that olefins (2) may be directly oxidised to epoxides (5) using ruthenium-based systems (see Yudin, Andrei K, Journal of Organic Chemistry (2001) 66(13), 4713-4718 and references therein) or by using meta chloroperbenzoic acid (Florez-Alvarez, Jose, Tetrahedron Letters (2002) 43(1), 171-174) or by the use of dimethyldioxirane (Bhoga, Umadevi, Tetrahedron Letters (2005) 46(31), 5239-5242).
(v) The epoxides may be reacted with amine HA-N(R20)R2 in solvents such as DMF or ethanol in the presence of bases such as sodium or potassium carbonate, disodium hydrogen phosphate or triethylamine to give the ethanolamines (6) (compounds of formula (IIA) in which L2 is LCH2CH(OH) and L is -A-N(R20)R2′). It is not essential or even necessary to isolate epoxide (5) as reaction of sulphonates (4) and amines with, for example, potassium carbonate as base, can afford the ethanolamines (6) with presumed in situ formation and reaction of epoxides (5) (see Bonini, Carlo, Tetrahedron (2005), 61(27), 6580-6589).
(vi) Alternatively, allyl derivatives (2) may be prepared from methyl heterocycles (7) by bromination with N-bromosuccinimide with catalysis from dibenzoyl peroxide or 2,2′-azodiisobutyronitrile (see Aguirre, Gabriela et al, Bioorganic & Medicinal Chemistry (2005) 13(23), 6324-6335) to give bromomethyl derivatives (8).
(vii) The bromomethyl derivatives (8) can be converted to the allyl analogues (2) by a variety of reagents, including vinyltributyltin (see Crawforth, Catherine et al, Tetrahedron Letters (2004) 45(3), 461-465), vinylmagnesium bromide with copper (I) iodide catalysis (see Esumi, Tomoyuki et al, Bioorganic & Medicinal Chemistry Letters (2004) 14(10), 2621-2625) and organoboron reagents (for a related example see Langle, Sandrine et al, Tetrahedron Letters (2003) 44(52), 9255-9258)
Intermediates such (1) and (7) may be prepared by the following schemes.
For the quinoxalinone system (Z1=N), Scheme 2 may be employed to give a methyl derivative (7):
Aniline (XVII) is converted by acylation to the chloroacetamide (XVI), which is nitrated to give (XV) and then hydrolysed to give the nitroaniline (XIV). This is converted into the cyanoacetamide (XIII) by treatment with cyanoacetic acid and phosphorus pentachloride (by the method of S. T. Hazeldine et al, J. Med. Chem., (2002) 44, 1758). Alternatively, the nitro-chloroacetamide (XV) may be treated with potassium cyanide to give cyanoacetamide (XIII) directly. Cyclisation with sodium hydroxide in pyridine gives a cyanoquinoxalinone-N-oxide (XII) which is reduced by sodium dithionite with loss of the cyano group to give a compound of formula (XI). This is first chlorinated with phosphorus oxychloride and then treated with sodium methoxide to give (X) or (XI) can be methylated with (trimethylsilyl)diazomethane in the presence of triethylamine. This route is particularly suitable for R1a=F.
For the quinolinone system where Z1 and Z2 are both carbon, Scheme 3 may be employed to give a methyl derivative (7):
The aniline (XVII) is converted to the cinnamide (XI), which is cyclised with aluminium chloride (with loss of the phenyl moiety—See M. C. Elliot et al. J. Med. Chem. 47 (22), 5405-5417 (2004), S. R. Inglis et al. Synlett, 5, 898-900 (2004), to give (VIII). This is selectively O-alkylated with e.g. methyl iodide to give (VII).
For the quinolinone system where Z1 and Z2 are both carbon, Scheme 4 may be employed to give a bromo derivative (1):
Aniline (VI) can be reacted with cinnamoyl chloride to give (V) which can subsequently be cyclised (for an example of this procedure see Cottet, F.; Marull, M.; Lefebvre, O.; Schlosser, M European Journal of Organic Chemistry (2003), 8, 1559) to give (IV). This can be converted into the bromo-quinoline (III) by o-methylation under standard conditions (see for examples Smith, M. B.; March, J. M. Advanced Organic Chemistry, Wiley-Interscience).
The naphthyridinone bromide (1) where Z1 and Z2 are both N is known in the literature, see for example WO 2004058144.
For the naphthyridone bromide (1) where Z1 and Z2 are both N and R1a is fluorine, Scheme 5 may be employed:
Carboxylic acid (XVIII) is converted to methoxy intermediate (XIX) and then to the protected aminopyridine (XX) via a Curtius type rearrangement under standard conditions (see for examples Smith, M. B.; March, J. M. Advanced Organic Chemistry, Wiley-Interscience). Enol ether (XXI) is formed via a Stille coupling using the procedure of Fu et al (for a reference see Littke, A. F.; Schwartx, L.; Fu, G. C. J. Am. Chem. Soc. 2002, 124, 6343). Reaction of (XXI) with Selectfluor® 1-chloromethyl-4-fluoro-1,4-diazoniabicyclo[2.2.2]octane gives fluoro-ketone (XXII) (see for examples Lal, G. S.; Pez, G. P.; Syvret, R. G. Chem. Rev, 1996, 96, 1737). Conversion to the enamino-ketone (XXIII), deprotection to give (XIV) and finally bromination gives intermediate (XXV) under standard conditions (see for examples Smith, M. B.; March, J. M. Advanced Organic Chemistry, Wiley-Interscience). This route is particularly suitable for R1b=F.
Interconversions of Z1, Z2, R1a, R1b, R2, A and R5 are conventional. In compounds which contain an optionally protected hydroxy group, suitable conventional hydroxy protecting groups which may be removed without disrupting the remainder of the molecule include acyl and alkylsilyl groups. N-protecting groups are removed by conventional methods.
Interconversion of R1a and R1b groups may be carried out conventionally, on compounds of formula (I), (IIA) or (IIB). For example R1a or R1b methoxy is convertible to R1a or R1b hydroxy by treatment with lithium and diphenylphosphine (general method described in Ireland et al, J. Amer. Chem. Soc., 1973, 7829) or HBr. Alkylation of the hydroxy group with a suitable alkyl derivative bearing a leaving group such as halide, yields R1a or R1b substituted alkoxy. R1a halogen is convertible to other R1a by conventional means, for example to hydroxy, alkylthiol (via thiol) and amino using metal catalysed coupling reactions, for example using copper as reviewed in Synlett (2003), 15, 2428-2439 and Angewandte Chemie, International Edition, 2003, 42(44), 5400-5449. R1b halo such as bromo may be introduced by the method of M. A. Alonso et al, Tetrahedron 2003, 59(16), 2821. R1a or R1b halo such as bromo may be converted to cyano by treatment with copper (I) cyanide in N,N-dimethylformamide. R1a or R1b carboxy may be obtained by conventional hydrolysis of R1a or R1b cyano, and the carboxy converted to hydroxymethyl by conventional reduction.
Compounds of formula HA-N(R20)R2′ are known compounds or may be prepared analogously to known compounds, see for example WO2004/035569, WO2004/089947, WO02/08224, WO02/50061, WO02/56882, WO02/96907, WO2003087098, WO2003010138, WO2003064421, WO2003064431, WO2004002992, WO2004002490, WO2004014361, WO2004041210, WO2004096982, WO2002050036, WO2004058144, WO2004087145, WO2003082835, WO2002026723, WO06002047, WO06014580, WO06134378 and WO06137485.
As shown in Scheme 6, the hydroxy-aminomethylpyrrolidines of formula (xiii) (HA-NH(R20), A is (ii), X is CR4R8, W1 is a bond, W2 and W3 are both CH2, R4 and R7 are H and R8 is OH) can be prepared from doubly protected chiral intermediate (xvi), separated by preparative HPLC. The benzyloxycarbonyl protecting group is removed by hydrogenation to give (xv) and the amino function converted to a trifluoroacetamide (xiv). The t-butoxycarbonyl (Boc) protecting group is removed with HCl to give the pyrrolidine hydrochloride salt (xiii).
The intermediate (xvi) may be prepared by the general method of Scheme 7:
Reagents and conditions: (a) N-Hydroxybenzylamine hydrochloride, paraformaldehyde, toluene, EtOH, 80° C.; (b) Pd(OH)2, H2 (50 psi), MeOH, room temperature; (c) Benzyloxycarbonyl-succinimide, Et3N, dichloromethane, room temperature.
In Scheme 8 the aminomethylpyrrolidine of formula (xvii) (HA-NH(R20), A is (ii), X is CR4R8, W1 is a bond, W2 and W3 are both CH2, R4, R7 and R8 are all H) can be prepared from commercially available Boc-protected aminomethylpyrrolidine, and converted to the trifluoroacetamide.
The aminomethylmorpholine intermediate of formula (xxi) (HA-NH(R20), A is (ii), X is O, W1, W2 and W3 are each CH2) may be prepared from a chiral dichlorobenzyl intermediate (xxiii) (WO2003082835) (Scheme 9) by first protecting the amino function with a Boc-protecting group (xxii), removing the dichlorobenzyl group by hydrogenation to give (xxi), protecting the morpholine N-atom with a benzyloxycarbonyl group (to allow purification by chromatography) (xx), and hydrogenation to afford the required morpholine derivative (xxi).
A method to prepare the pyrimidinyloxazinone unit R5 (C), where Y3=O, R10=H) is illustrated in Scheme 10.
A suitably protected ethyl glycolate (THP-protected in this example, 1) is formylated using ethyl formate and a base such as NaH in THF or diethyl ether. The intermediate formyl enolate 2 is then directly reacted with an amidine, in this case the (2E)-3-phenyl-2-propenimidamide 3, giving the pyrimidinone 4. Pyrimidinone 4 is converted to a trifluoromethansulfonate ester (5) which is then reacted with ammonia in a suitable solvent, such as 1,4-dioxane, providing amine 6. The amino alcohol 7 is then obtained by removing the THP-protecting group of 6 with acid in methanol. Treatment of 7 with a base and an ester of a halo-acetate in an alcohol solvent such as absolute ethanol, provides the bicyclic intermediate 8 directly. This transformation may be accomplished using a base such as potassium tert-butoxide and the alkylating agent ethyl bromoacetate. An amine base such as triethylamine may also be employed as an alternative to the alkoxide base illustrated herein (for similar examples see N. V. Sazonov and T. S. Safonova, Khimiya Geterotsiklicheskikh Soedinenii, 1971, 1285-1288). The final aldehyde intermediate 9 is then obtained via oxidative cleavage of the phenylethenyl side chain. One method to achieve this is by reacting 8 with NaIO4, in a mixture of 1,4-dioxane-water, with a catalytic amount of OSO4. Other methods, such as ozonolysis, may also be suitable to achieve the desired transformation.
Pyrimidine dihydropyridone aldehyde (R5 (C) where Y3=CH2 and R10=Cl) may be prepared as illustrated in Scheme 11.
By reacting the anion of dimethyl malonate (10) with ethyl acrylate (11), the triester 12 is obtained. Condensing 12 with (2E)-3-phenyl-2-propenimidamide (3), in the presence of a base, leads to the dihydroxypyrimidine 13. Triethylamine in EtOH can be used to carry out this transformation, however the preferred conditions utilize NaOMe in MeOH. It should be noted that under these latter conditions the methyl ester of 13 (R=Me) is obtained whereas the ethyl ester is preserved using the former conditions. Either ester form, methyl or ethyl, can be used to carry out the remaining steps of the synthesis. Treating 13 with POCl3 provides the dichloropyrimidine 14. Heating 14 in a sealed tube in the presence of NH4OH usually yields a mixture of the components 15, 16, and 17 with 15 and 16 predominating. Subsequently, intermediate 15 can be converted to 16 by treating with K2CO3 in MeOH. In addition, 17 can be recycled to 15 (R=Et) by treatment with ethanolic HCl. The preparation of the aldehyde 18 is then completed via oxidative cleavage of the olefin side chain of 16 using either OSO4 and NaIO4, or by ozonolysis.
Scheme 12 illustrates one convenient method to remove the chlorine substituent found in 18 in order to obtain the des-chloro aldehyde 20 (R5 (C) where Y3=CH2 and R10=H). This can be achieved by first protecting the aldehyde group of 18 by forming the dimethyl acetal using p-toluene sulfonic acid (p-TsOH) and MeOH, providing 19. The chlorine is then be removed by hydrogenation using Pd—C catalysis under an atmosphere of H2. Treatment with aqueous acid, such as TFA and water, once again liberates the aldehyde group, thus providing 20.
Scheme 13 illustrates a method to prepare analogs incorporating alternative substituents at the 4-position on the pyrimidine ring, for example for R5 (C) where Y3 CH2 and R10=OMe or Me. These analogs can be prepared from the previously described intermediate 16 using a variety of well known methods. Illustrated in Scheme 13 is the preparation of the 4-methoxy and the 4-methyl derivatives, however similar or other methods may be employed to incorporate a wide range of substituents. As shown below, 16 can be treated with NaOMe in refluxing methanol to provide the methoxy-containing intermediate 21A. The methyl group can be prepared from 16 via a Pd-mediated reaction with methyl boronic acid, thus affording 21B. The aldehyde functional group is once again liberated by oxidative cleavage of the olefin side chain using methods such as ozonolysis, or by reaction with OsO4 and NaIO4, to provide 22A and 22B.
The pyrimidine oxazinone aldehyde unit needed to prepare examples of R5 (C) where Y3=O and R10=Cl, is shown in Scheme 14 starting from dimethyl diazomalonate (23), prepared according to Peace, Carman, Wulfinan, Synthesis, 658-661, (1971). Reaction of 23 with ethyl glycolate under rhodium catalysis provides the substituted malonate 24. The pyrimidine ring system is constructed through the reaction of 24 with (2E)-3-phenyl-2-propenimidamide (3), and sodium methoxide to give 25. Intermediate 25 is isolated as the carboxylic acid as the methyl ester is hydrolyzed under the sodium methoxide reaction conditions. Treatment of 25 with POCl3 followed by the addition of MeOH provides dichloride-methyl ester 26. Exchanging one of the chlorines with ammonia can be accomplished by treating 26 with NH4OH, also providing the primary amide, which is then converted to the ethyl ester 27 with HCl and EtOH. Formation of the oxazinone ring can be carried out by treating 27 with a base such as K2CO3 in a polar solvent such as DMF. Heating is usually required to complete the conversion to the bicyclic system 28. Conversion to the aldehyde can be achieved by oxidative cleavage of the 2-phenylethenyl side chain. In this particular example, the side chain of 28 is reacted with OSO4 and NaIO4 to give aldehyde 29.
Further details for the preparation of compounds of formula (I) are found in the examples.
The antibacterial compounds according to the invention may be formulated for administration in any convenient way for use in human or veterinary medicine, by analogy with other antibacterials.
The pharmaceutical compositions of the invention include those in a form adapted for oral, topical or parenteral use and may be used for the treatment of bacterial infection in mammals including humans.
The composition may be formulated for administration by any route. The compositions may be in the form of tablets, capsules, powders, granules, lozenges, creams or liquid preparations, such as oral or sterile parenteral solutions or suspensions.
The topical formulations of the present invention may be presented as, for instance, ointments, creams or lotions, eye ointments and eye or ear drops, impregnated dressings and aerosols, and may contain appropriate conventional additives such as preservatives, solvents to assist drug penetration and emollients in ointments and creams.
The formulations may also contain compatible conventional carriers, such as cream or ointment bases and ethanol or oleyl alcohol for lotions. Such carriers may be present as from about 1% up to about 98% of the formulation. More usually they will form up to about 80% of the formulation.
Tablets and capsules for oral administration may be in unit dose presentation form, and may contain conventional excipients such as binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, or polyvinylpyrrolidone; fillers, for example lactose, sugar, maize-starch, calcium phosphate, sorbitol or glycine; tabletting lubricants, for example magnesium stearate, talc, polyethylene glycol or silica; disintegrants, for example potato starch; or acceptable wetting agents such as sodium lauryl sulphate. The tablets may be coated according to methods well known in normal pharmaceutical practice. Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives, such as suspending agents, for example sorbitol, methyl cellulose, glucose syrup, gelatin, hydroxyethyl cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenated edible fats, emulsifying agents, for example lecithin, sorbitan monooleate, or acacia; non-aqueous vehicles (which may include edible oils), for example almond oil, oily esters such as glycerine, propylene glycol, or ethyl alcohol; preservatives, for example methyl or propyl p-hydroxybenzoate or sorbic acid, and, if desired, conventional flavouring or colouring agents.
Suppositories will contain conventional suppository bases, e.g. cocoa-butter or other glyceride.
For parenteral administration, fluid unit dosage forms are prepared utilizing the compound and a sterile vehicle, water being preferred. The compound, depending on the vehicle and concentration used, can be either suspended or dissolved in the vehicle. In preparing solutions the compound can be dissolved in water for injection and filter sterilised before filling into a suitable vial or ampoule and sealing.
Advantageously, agents such as a local anaesthetic, preservative and buffering agents can be dissolved in the vehicle. To enhance the stability, the composition can be frozen after filling into the vial and the water removed under vacuum. The dry lyophilized powder is then sealed in the vial and an accompanying vial of water for injection may be supplied to reconstitute the liquid prior to use. Parenteral suspensions are prepared in substantially the same manner except that the compound is suspended in the vehicle instead of being dissolved and sterilization cannot be accomplished by filtration. The compound can be sterilised by exposure to ethylene oxide before suspending in the sterile vehicle. Advantageously, a surfactant or wetting agent is included in the composition to facilitate uniform distribution of the compound.
The compositions may contain from 0.1% by weight, preferably from 10-60% by weight, of the active material, depending on the method of administration. Where the compositions comprise dosage units, each unit will preferably contain from 50-1000 mg of the active ingredient. The dosage as employed for adult human treatment will preferably range from 100 to 3000 mg per day, for instance 1500 mg per day depending on the route and frequency of administration. Such a dosage corresponds to 1.5 to 50 mg/kg per day. Suitably the dosage is from 5 to 20 mg/kg per day.
No toxicological effects are indicated when a compound of formula (I) or a pharmaceutically acceptable salt, solvate and/or N-oxide thereof is administered in the above-mentioned dosage range.
The compound of formula (I) may be the sole therapeutic agent in the compositions of the invention or a combination with other antibacterials. If the other antibacterial is a β-lactam then a β-lactamase inhibitor may also be employed.
Compounds of formula (I) may be used in the treatment of bacterial infections caused by a wide range of organisms including both Gram-negative and Gram-positive organisms, such as upper and/or lower respiratory tract infections, skin and soft tissue infections and/or urinary tract infections. Some compounds of formula (I) may be active against more than one organism. This may be determined by the methods described herein.
The following examples illustrate the preparation of certain compounds of formula (I) and the activity of certain compounds of formula (I) against various bacterial organisms.
LC-MS=Liquid chromatography mass spectrometry.
Rt=retention time
Certain reagents are also abbreviated herein. DMF refers to N,N-dimethylformamide, TFA refers to trifluoroacetic acid, THF refers to tetrahydrofuran and DCM refers to dichloromethane. NBS refers to N-bromosuccinimide.
Proton nuclear magnetic resonance (1H NMR) spectra were recorded at 400, or 250 MHz, and chemical shifts are reported in parts per million (6) downfield from the internal standard tetramethylsilane (TMS). Abbreviations for NMR data are as follows: s=singlet, d=doublet, t=triplet, q=quartet, m=multiplet, dd=doublet of doublets, dt=doublet of triplets, app=apparent, br=broad. CDCl3 is deuteriochloroform, DMSO-d6 is hexadeuteriodimethylsulfoxide, and CD3OD is tetradeuteriomethanol. Mass spectra were obtained using electrospray (ES) ionization techniques. All temperatures are reported in degrees Celsius.
MP-carbonate resin refers to macroporous triethylammonium methylpolystyrene carbonate (Argonaut Technologies). Amberlyst®A21 is a weakly basic, macroreticular resin with alkyl amine functionality, ®Registered trademark of Rohm & Haas Co. SCX is an ion exchange column containing strong cation exchange resin (benzene sulfonic acid) supplied by Varian, USA.
Chiralpak AS-H columns comprise of a chiral adsorbent based on amylose tris [(S)-alpha methylbenzylcarbamate]coated onto 5 um silica gel (21 mm ID×250 mm L, Chiral Technologies, Inc). Measured retention times are dependent on the precise conditions of the chromatographic procedures. Where quoted below in the Examples they are indicative of the order of elution. Chiralpak IA column comprise of silica for preparative column (5 um particle size, 21 mm ID×250 mm L) immobilized with Amylose tris(3,5-dimethylphenylcarbamate).
AD mix alpha is prepared by mixing potassium osmate (K2OsO4.2H2O) (0.52 g), (3a,9R,3′″a,4′″b,9′″R)-9,9′-[1,4-phthalazinediylbis(oxy)]bis[6′-(methyloxy)-10,11-dihydrocinchonan] [(DHQ)2PHAL] (5.52 g), then adding potassium ferricyanide [K3Fe(CN)6] (700 g) and powdered potassium carbonate (294 g). This mixture is stirred in a blender for 30 minutes. This provides approximately 1 kg of AD mix alpha, which is commercially available from Aldrich. See K. Barry Sharpless et al, J. Org. Chem., 1992, 57 (10), 2771. AD mix beta is the corresponding mixture prepared with (9S,9′″S)-9,9′-[1,4-phthalazinediylbis(oxy)]bis[6′-(methyloxy)-10,11-dihydrocinchonan] [(DHQD)2PHAL]. Where AD mix alpha and beta is referred to, this is a 1:1 mixture of the alpha and beta mix.
Reactions involving metal hydrides including lithium hydride, lithium aluminium hydride, di-isobutylaluminium hydride, sodium hydride, sodium borohydride, sodium triacetoxyborohydride, (polystyrylmethyl)trimethylammonium cyanoborohydride are carried out under argon or other inert gas.
As will be understood by the skilled chemist, references to preparations carried out in a similar manner to, or by the general method of, other preparations, may encompass variations in routine parameters such as time, temperature, workup conditions, minor changes in reagent amounts etc.
A suspension of 8-bromo-7-fluoro-2-(methyloxy)-1,5-naphthyridine (20 g, 77.8 mmol), (for a synthesis, see WO2004058144, Example 53(g)) in degassed 1,4-dioxane (200 ml) was treated under argon with tris(dibenzylideneacetone)dipalladium(0) (0.71 g, 0.8 mmol), bis(tri-tert-butylphosphine)palladium(0) (0.8 g, 1.6 mmol), allyltributylstannane (24.7 ml, 80 mmol) and cesium fluoride (26 mg, 0.2 mmol) and the mixture heated at 70° C. for 1 hour. The mixture was filtered through a plug of Keiselguhr, washing with ethyl acetate (200 ml). The filtrate was treated with half saturated brine (200 ml) and the phases separated. The aqueous phase was further extracted with ethyl acetate (2×100 ml). The combined organic extracts were dried and evaporated affording a brown oil (44 g). This material was chromatographed eluting with 0-100% ethyl acetate in hexane affording a yellow oil (17 g, 100%).
MS (+ve ion electrospray) m/z 219 (MH+).
A solution of 7-fluoro-2-(methyloxy)-8-(2-propen-1-yl)-1,5-naphthyridine (17 g, 78 mmol) in tert-butanol (500 ml) was treated with water (500 ml) and the cloudy suspension cooled in an ice bath. An equal mixture of AD mix alpha and beta (50 g of each) was added portionwise over 45 minutes. The mixture was stirred at ambient temperature for 18 hours. More AD mix alpha and beta (20 g of each) was added and the mixture stirred for a further 5 days. The phases were separated and the aqueous phase was extracted with 10% methanol in DCM (4×250 ml). The combined organic extracts were not homogenous and a second phase separation was carried out. The organic phase was dried (sodium sulphate) and evaporated affording a white solid (18 g, 92%).
MS (+ve ion electrospray) m/z 253 (MH+).
A suspension of (2R/S)-3-[3-fluoro-6-(methyloxy)-1,5-naphthyridin-4-yl]-1,2-propanediol (18 g, 71 mmol) in DCM (400 ml), THF (400 ml) and DMF (400 ml) was treated at 15° C. with triethylamine (15 ml, 107 mmol), dibutyltin oxide (0.9 g, 3.5 mmol) and para-toluenesulphonyl chloride (13.6 g, 71 mmol). After 12 hours the mixture was treated with saturated aqueous sodium bicarbonate solution (300 ml) and the phases separated. The aqueous phase was further extracted with 10% methanol in dichloromethane (3×200 ml). The combined organic extracts were dried (sodium sulphate) and evaporated affording a yellow oil. Chromatography eluting with 0-2% methanol in DCM afforded some pure material which was isolated by trituration with ether followed by filtration affording a solid (18.4 g). The filtrate was combined with the impure column fractions and evaporated. Chromatography eluting with 0-10% methanol in DCM afforded some further material which was isolated by trituration with ether followed by filtration affording a solid (3 g). Combined yield=21.4 g (74%).
MS (+ve ion electrospray) m/z 407 (MH+).
A suspension of (2R/S)-3-[3-fluoro-6-(methyloxy)-1,5-naphthyridin-4-yl]-2-hydroxypropyl 4-methylbenzenesulfonate (21.3 g, 52.5 mmol), 1,1-dimethylethyl 4-piperidinylcarbamate (21 g, 105 mmol) in ethanol/DMF (250 ml/65 ml) and sodium carbonate (16.7 g, 156 mmol) was heated to 40° C. under argon for 2 days. The mixture was filtered through Keiselguhr and evaporated. The residue was partitioned between DCM and water (100 ml each) and the aqueous phase further extracted with DCM (50 ml). The combined extracts were added to a silica gel column which was subject to chromatography eluting with 0-2% methanol in ethyl acetate. Impure fractions were combined and rechromatographed eluting with 0-20% methanol in ethyl acetate. All pure fractions were combined and evaporated to afford a foam (9.7 g, 42%).
MS (+ve ion electrospray) m/z 435 (MH+).
A solution of 1,1-dimethylethyl (1-{(2R/S)-3-[3-fluoro-6-(methyloxy)-1,5-naphthyridin-4-yl]-2-hydroxypropyl}-4-piperidinyl)carbamate (2.0 g, 4.6 mmol) in DCM (100 ml) was cooled to 0° C. (ice-salt bath) under argon and treated with pyridine (0.82 ml, 10.1 mmol) then over 10 minutes with trifluoromethanesulphonic anhydride (0.93 ml, 5.5 mmol). The mixture was allowed to come to room temperature overnight, then recooled to 4° C. and treated with a second portion of trifluoromethanesulphonic anhydride (0.19 ml, 1.1 mmol). Again, the mixture was allowed to come to room temperature overnight. The dark reaction mixture was poured into water (500 ml). The phases were separated and the aqueous phase further extracted with DCM (4×50 ml). The combined organic extracts were dried and evaporated affording a dark foam (3 g). Chromatography eluting with 0-100% ethyl acetate in hexane then 0-50% methanol in ethyl acetate afforded an approximately equal mixture of starting material and 1,1-dimethylethyl {1-[(5R/S)-3-fluoro-8-oxo-5,6-dihydro-4H,8H-pyrido[3,2,1-de]-1,5-naphthyridin-5-yl]-4-piperidinyl}carbamate (451 mg) then 1,1-dimethylethyl (1-{[(5R/S)-3-fluoro-7-oxo-4,5-dihydro-7H-pyrrolo[3,2,1-de]-1,5-naphthyridin-5-yl]methyl}-4-piperidinyl)carbamate (487 mg, 26%).
MS (+ve ion electrospray) m/z 403 (MH+).
The above mixture of starting material and 1,1-dimethylethyl {1-[(5R/S)-3-fluoro-8-oxo-5,6-dihydro-4H,8H-pyrido[3,2,1-de]-1,5-naphthyridin-5-yl]-4-piperidinyl}carbamate (451 mg) was dissolved in dimethylsulphoxide and treated with acetonitrile. On standing 1,1-dimethylethyl {1-[(5R/S)-3-fluoro-8-oxo-5,6-dihydro-4H,8H-pyrido[3,2,1-de]-1,5-naphthyridin-5-yl]-4-piperidinyl}carbamate crystallised out and was isolated by filtration and drying in vacuo as a white crystalline solid (35 mg).
MS (+ve ion electrospray) m/z 403 (MH+).
A solution of 1,1-dimethylethyl (1-{[(5R/S)-3-fluoro-7-oxo-4,5-dihydro-7H-pyrrolo[3,2,1-de]-1,5-naphthyridin-5-yl]methyl}-4-piperidinyl)carbamate (300 mg, 0.75 mmol) in TFA/DCM (3 ml/3 ml) was stirred for 1 hour then evaporated. The residue was dissolved in 1/1 DCM/methanol and treated with MP-carbonate resin (until a moistened pH indicator strip indicated ca pH8). Filtration, washing with methanol and evaporation afforded a brown oily solid (175 mg, 78%).
MS (+ve ion electrospray) m/z 303 (MH+).
A solution of (5R/S)-5-[(4-amino-1-piperidinyl)methyl]-3-fluoro-4,5-dihydro-7H-pyrrolo[3,2,1-de]-1,5-naphthyridin-7-one (66 mg, 0.22 mmol) and 2,3-dihydro[1,4]dioxino[2,3-c]pyridine-7-carboxaldehyde (for a synthesis see WO2004058144, Example 2(c) or WO03/087098, Example 19(d)) (36 mg, 0.22 mmol) in chloroform/methanol (2.5 ml/0.25 ml) was treated with sodium triacetoxyborohydride (140 mg, 6.6 mmol) and stirred under argon for 2 hours. The mixture was treated with saturated aqueous sodium bicarbonate solution (5 ml) and 10% methanol in DCM (5 ml) and the phases separated. The aqueous phase was further extracted with 10% methanol in dichloromethane (3×5 ml). The combined organic extracts were dried (sodium sulphate) and evaporated affording a yellow oil. Chromatography eluting with 0-30% methanol in DCM afforded the free bases of the title compounds (54 mg, 55%).
δH (CD3OD, 250 MHz) 1.20-1.50 (2H, m), 1.75-1.95 (2H, m), 2.10-2.30 (2H, m), 2.42-2.60 (1H, m), 2.60-2.75 (2H, m), 3.00-3.15 (2H, m), 3.50-3.70 (2H, m), 3.80 (2H, s), 4.25-4.40 (4H, m), 5.10-5.20 (1H, m), 6.75 (1H, d), 6.95 (1H, s), 7.95 (1H, d), 8.00 (1H, s), 8.40 (1H, s)
MS (+ve ion electrospray) m/z 452 (MH+).
This material was converted to the corresponding dihydrochloride salt (54 mg). Chromatography of a portion (14 mg) of this material on a Chiralpak AS-H column eluting with 85:15:0.1 acetonitrile:methanol:isopropylamine afforded 4.8 mg of each of the separate free bases of the title compounds (Rt Enantiomer 1, 4.6 minutes, Rt Enantiomer 2, 8.7 minutes). Each free base was then converted to the separate title compounds with one equivalent of hydrochloric acid.
This was prepared by a slight variation on the method of Kasnar et al, Nucleosides & Nucleotides (1994), 13(1-3), 459-79.
Hydrazine sulphate salt (51 g) was suspended in water (250 ml), heated to reflux and bromomaleic anhydride (90.38 g) was added dropwise. The mixture was heated at reflux for 4 hours then cooled to room temperature. The reaction was repeated with 29 g hydrazine sulphate, 53 g bromomaleic anhydride and 130 ml water. The precipitates were collected by filtration, washed with water and acetone and dried as a combined batch in vacuo to afford 4-bromo-1,2-dihydro-3,6-pyridazinedione as a white solid (113 g).
The solid in two batches was treated with phosphorus oxychloride (2×200 ml) and heated to reflux for 3.5 hours. The mixture was cooled, evaporated and azeotroped with toluene. The residue was partitioned between dichloromethane and saturated aqueous sodium bicarbonate solution and extracted with DCM twice more. The organic extracts were dried and evaporated. This residue was re-dissolved in dichloromethane, and chromatographed on silica gel (300 g) (DCM as eluent) to give a white solid (101.5 g, 87%).
(LC/MS analysis showed ca 20-30% impurity, isomers of bromo-dichloropyridazine).
MS (+ve ion electrospray) m/z 184/185/186 (MH+), trichloropyridazine.
MS (+ve ion electrospray) m/z 228/229/231 (MH+), bromo-dichloropyridazine.
A solution of ethylene glycol (55 ml) in tetrahydrofuran (200 ml) was treated at around 0° C. (ice bath cooling) with sodium hydride (60% dispersion in oil, 5.9 g) over 40 minutes. After the addition was complete, 3,4,6-trichloropyridazine (27 g) containing isomers of bromo-dichloropyridazine as impurity was added portionwise and washed in with more dry THF (50 ml) and the mixture was stirred at 0° C. for 1 hour and then at room temperature overnight. The mixture was concentrated (to ⅓ volume) then diluted with aqueous sodium bicarbonate solution and extracted with chloroform (5×) and ethyl acetate (3×). The combined organic extracts were washed with water, dried over sodium sulphate and evaporated and the solids filtered off and washed with CHCl3 (×3) and dried in a vacuum oven overnight at 40° C. affording a white solid (25.5 g, 83%), containing some bromo-derivative (10-15%).
MS (+ve ion electrospray) m/z 209/211 (MH+).
MS (+ve ion electrospray) m/z 255/7 (MH+), bromo-derivative.
A solution of 2-[(3,6-dichloro-4-pyridazinyl)oxy]ethanol (5.5 g) in tetrahydrofuran (4.5 litres) was treated with sodium hydride (60% dispersion in oil, 8 g) and heated at 80 deg C. for 72 hours. The reaction mixture was quenched with wet tetrahydrofuran then ice and evaporated to dryness. The residue was chromatographed on silica eluting with 0-100% ethyl acetate in hexane affording a white solid (2.5 g, 55%) containing some bromo species (5%).
MS (+ve ion electrospray) m/z 173 (MH+).
A solution of 3-chloro-6,7-dihydro[1,4]dioxino[2,3-c]pyridazine (2.5 g, 14.5 mmol) in dimethoxyethane (75 ml) was degassed under argon then tetrakis(triphenylphosphine)palladium (0) (500 mg), potassium carbonate (1.9 g), 2,4,6-trivinylcyclotriboroxane pyridine complex (commercially available from Astatech Ltd) and water (10 ml) were added. The mixture was heated at 95° C. for 16 hours. The mixture was treated with aqueous sodium bicarbonate solution and evaporated to dryness. The residue was chromatographed on silica eluting with 0-100% ethyl acetate in hexane affording a white solid (1.7 g, 70%).
MS (+ve ion electrospray) m/z 165 (MH+).
A solution of 3-ethenyl-6,7-dihydro[1,4]dioxino[2,3-c]pyridazine (1.4 g) in 1,4-dioxane/water (100 ml/35 ml) was treated with an aqueous solution of osmium tetroxide (4% w/v, 8 ml) and sodium periodate (3.9 g). After 5 hours the mixture was evaporated onto silica and chromatographed eluting with 0-100% ethyl acetate in hexane affording a white solid (820 mg, 60%).
MS (+ve ion electrospray) m/z 165 (MH+).
A solution of (5R/S)-5-[(4-amino-1-piperidinyl)methyl]-3-fluoro-4,5-dihydro-7H-pyrrolo[3,2,1-de]-1,5-naphthyridin-7-one (58 mg, 0.19 mmol) and 6,7-dihydro[1,4]dioxino[2,3-c]pyridazine-3-carbaldehyde (32 mg, 0.19 mmol) in chloroform/methanol (2.5 ml/0.25 ml) was treated with sodium triacetoxyborohydride (120 mg, 5.7 mmol) and stirred under argon for 2 hours. The mixture was treated with saturated aqueous sodium bicarbonate solution (5 ml) and 10% methanol in DCM (5 ml) and the phases separated. The aqueous phase was further extracted with 10% methanol in dichloromethane (3×5 ml). The combined organic extracts were dried (sodium sulphate) and evaporated. Chromatography eluting with 0-30% methanol in DCM afforded the free bases of the title compounds (43 mg, 50%).
δH (CD3OD, 250 MHz) 1.20-1.50 (2H, m), 1.75-1.95 (2H, m), 2.10-2.30 (2H, m), 2.42-2.55 (1H, m), 2.60-2.75 (2H, m), 3.00-3.15 (2H, m), 3.50-3.70 (2H, m), 3.90 (2H, s), 4.40-4.55 (4H, m), 5.10-5.20 (1H, m), 6.78 (1H, d), 7.25 (1H, s), 7.95 (1H, d), 8.40 (1H, s)
MS (+ve ion electrospray) m/z 453 (MH+).
This material was converted to the corresponding dihydrochloride salt (45 mg) Chromatography of a portion (18 mg) of this material on a Chiralpak AS-H column eluting with 90:10:0.1 acetonitrile:methanol:isopropylamine afforded 6.0 mg of each of the separate free bases of the title compounds (Rt Enantiomer 1, 3.9 minutes, Rt Enantiomer 2, 11.3 minutes). Each free base was then converted to the separate title compounds with one equivalent of hydrochloric acid.
A solution of (5R/S)-5-[(4-amino-1-piperidinyl)methyl]-3-fluoro-4,5-dihydro-7H-pyrrolo[3,2,1-de]-1,5-naphthyridin-7-one (58 mg, 0.19 mmol) and [1,3]oxathiolo[5,4-c]pyridine-6-carbaldehyde (for a synthesis, see WO2004058144, Example 61) (32 mg, 0.19 mmol) in chloroform/methanol (2.5 ml/0.25 ml) was treated with sodium triacetoxyborohydride (120 mg, 5.7 mmol) and stirred under argon for 2 hours. The mixture was treated with saturated aqueous sodium bicarbonate solution (5 ml) and 10% methanol in DCM (5 ml) and the phases separated. The aqueous phase was further extracted with 10% methanol in dichloromethane (3×5 ml). The combined organic extracts were dried (sodium sulphate) and evaporated. Chromatography eluting with 0-30% methanol in DCM afforded the free bases of the title compounds (46 mg, 53%).
δH (CD3OD, 250 MHz) 1.20-1.50 (2H, m), 1.75-1.95 (2H, m), 2.10-2.30 (2H, m), 2.40-2.55 (1H, m), 2.60-2.75 (2H, m), 3.00-3.15 (2H, m), 3.50-3.70 (2H, m), 3.75 (2H, s), 5.10-5.20 (1H, m), 5.80 (2H, s), 6.78 (1H, d), 7.35 (1H, s), 7.80 (1H, s), 7.95 (1H, d), 8.40 (1H, s)
MS (+ve ion electrospray) m/z 454 (MH+).
This material was converted to the corresponding dihydrochloride salt (50 mg) Chromatography of a portion (12 mg) of this material on a Chiralpak AS-H column eluting with 90:10:0.1 acetonitrile:methanol:isopropylamine afforded 4.6 mg of each of the separate free bases of the title compounds (Rt Enantiomer 1, 5.4 minutes, Rt Enantiomer 2, 10.7 minutes). Each free base was then converted to the separate title compounds with one equivalent of hydrochloric acid.
A solution of (5R/S)-5-[(4-amino-1-piperidinyl)methyl]-3-fluoro-4,5-dihydro-7H-pyrrolo[3,2,1-de]-1,5-naphthyridin-7-one (57 mg, 0.19 mmol) and 2,3-dihydro[1,4]oxathiino[2,3-c]pyridine-7-carbaldehyde (34 mg, 0.19 mmol) (for a synthesis, see WO2004058144, Example 60(i)) in chloroform/methanol (2.5 ml/0.25 ml) was treated with sodium triacetoxyborohydride (120 mg, 5.7 mmol) and stirred under argon for 2 hours. The mixture was treated with saturated aqueous sodium bicarbonate solution (5 ml) and 10% methanol in DCM (5 ml) and the phases separated. The aqueous phase was further extracted with 10% methanol in dichloromethane (3×5 ml). The combined organic extracts were dried (sodium sulphate) and evaporated. Chromatography eluting with 0-30% methanol in DCM afforded the free bases of the title compounds (90 mg, 100%).
δH (CD3OD, 250 MHz) 1.30-1.50 (2H, m), 2.00-2.25 (2H, m), 2.50-2.70 (2H, m), 3.20-3.40 (4H, m), 3.60-4.00 (5H, m), 4.35 (2H, s), 4.50 (2H, t), 5.70 (1H, m), 6.95 (1H, d), 7.35 (1H, s), 8.10 (2H, m), (1H, d), 8.50 (1H, s)
MS (+ve ion electrospray) m/z 468 (MH+).
This material was converted to the corresponding dihydrochloride salt (69 mg). Chromatography of a portion (14 mg) of this material on a Chiralpak AS-H column eluting with 85:15:0.1 acetonitrile:methanol:isopropylamine afforded 3.8 mg of each of the separate free bases of the title compounds (Rt Enantiomer 1, 5.2 minutes, Rt Enantiomer 2, 11.1 minutes). Each free base was then converted to the separate title compounds with one equivalent of hydrochloric acid.
A solution of (5R/S)-5-[(4-amino-1-piperidinyl)methyl]-3-fluoro-4,5-dihydro-7H-pyrrolo[3,2,1-de]-1,5-naphthyridin-7-one (57 mg, 0.19 mmol) and 3-oxo-3,4-dihydro-2H-pyrido[3,2-b][1,4]thiazine-6-carboxaldehyde (37 mg, 0.19 mmol) (for a synthesis, see, WO2004058144, Example 7(d)) in chloroform/methanol (2.5 ml/0.25 ml) was treated with sodium triacetoxyborohydride (120 mg, 5.7 mmol) and stirred under argon for 2 hours. The mixture was treated with saturated aqueous sodium bicarbonate solution (5 ml) and 10% methanol in DCM (5 ml) and the phases separated. The aqueous phase was further extracted with 10% methanol in dichloromethane (3×5 ml). The combined organic extracts were dried (sodium sulphate) and evaporated. Chromatography eluting with 0-30% methanol in DCM afforded the free bases of the title compounds (44 mg, 48%).
δH (CD3OD, 250 MHz) 1.15-1.40 (2H, m), 1.70-1.95 (2H, m), 2.05-2.25 (2H, m), 2.40-2.50 (1H, m), 2.60-2.70 (2H, m), 2.95-3.10 (2H, m), 3.45-3.65 (4H, m), 3.75 (2H, s), 5.05-5.15 (1H, m), 6.75 (1H, d), 7.05 (1H, d), 7.70 (1H, d), 7.95 (1H, d), 8.40 (1H, s)
MS (+ve ion electrospray) m/z 481 (MH+).
This material was converted to the corresponding dihydrochloride salt (43 mg)
Chromatography of a portion (12 mg) of this material on a Chiralpak AS-H column eluting with 85:15:0.1 acetonitrile:methanol:isopropylamine afforded 3.1 mg of each of the separate free bases of the title compounds (Rt Enantiomer 1, 5.8 minutes, Rt Enantiomer 2, 9.1 minutes). Each free base was then converted to the separate title compounds with one equivalent of hydrochloric acid.
A solution of (5R/S)-5-[(4-amino-1-piperidinyl)methyl]-3-fluoro-4,5-dihydro-7H-pyrrolo[3,2,1-de]-1,5-naphthyridin-7-one (57 mg, 0.19 mmol) and 3-oxo-3,4-dihydro-2H-pyrido[3,2-b][1,4]oxazine-6-carboxaldehyde (for a synthesis, see WO2004058144, Example 1(1)) (34 mg, 0.19 mmol) in chloroform/methanol (2.5 ml/0.25 ml) was treated with sodium triacetoxyborohydride (120 mg, 5.7 mmol) and stirred under argon for 2 hours. The mixture was treated with saturated aqueous sodium bicarbonate solution (5 ml) and 10% methanol in DCM (5 ml) and the phases separated. The aqueous phase was further extracted with 10% methanol in dichloromethane (3×5 ml). The combined organic extracts were dried (sodium sulphate) and evaporated. Chromatography eluting with 0-30% methanol in DCM afforded the free base of the title compound (51 mg, 57%).
δH (DMSO-d6, 250 MHz) 1.10-1.35 (2H, m), 1.70-1.90 (2H, m), 2.00-2.22 (2H, m), 2.40-2.70 (3H, m), 2.90-3.00 (2H, m), 3.40-3.65 (4H, m), 3.70 (2H, s), 4.62 (2H, s), 5.05-5.15 (1H, m), 6.75 (1H, d), 7.00 (1H, d), 7.30 (1H, d), 7.95 (1H, d), 8.45 (1H, s)
MS (+ve ion electrospray) m/z 465 (MH+).
A solution of the above material was treated with 1M hydrochloric acid in methanol (0.3 ml) and evaporated to dryness affording the dihydrochloride salt.
(1) (2E)-N-(3-Fluoro-2-methylphenyl)-3-phenyl-2-propenamide A solution of cinnamyl chloride (100 g, 610 mmol) in ethyl acetate (400 ml) was added to a vigorously-stirred mixture of 3-fluoro-2-methylaniline (75 g, 400 mmol), saturated aqueous sodium bicarbonate (850 ml), ice (ca 100 g) and ethyl acetate (400 ml). After 1 hour the mixture was concentrated on a rotary evaporator (removing most of the ethyl acetate) and filtered, washing with water. The resulting white solid was dried in vacuo (˜160 g, 100%).
MS (+ve ion electrospray) m/z 256 (MH+).
A solution of (2E)-N-(3-fluoro-2-methylphenyl)-3-phenyl-2-propenamide (75 g, 305 mmol) in chlorobenzene (400 ml) was treated slowly with aluminium trichloride (163 g, 1.2 mol) over 10 minutes, with the temperature <30° C. The reaction was heated to 75° C. (internal temperature) for 1 hour. The mixture was allowed to cool (ca 40° C.), then added to excess ice with vigorous stirring and left to stand at room temperature overnight. The resulting oily precipitate was isolated by filtration and washing with water. Drying in vacuo afforded a light brown solid (42.5 g, 79%).
MS (+ve ion electrospray) m/z 178 (MH+).
Crude 7-fluoro-8-methyl-2(1H)-quinolinone (46 g, 260 mmol) was suspended in DMSO (300 ml), warmed to 35° C., then treated with potassium t-butoxide (32 g, 286 mmol), under argon (the internal temperature rose to 45° C.). After 15 minutes methyl iodide (21 ml, 48 g, 338 mmol) was added over 2 minutes. (The internal temperature rose to 60° C.). The mixture was added to water (2 litres) and extracted with hexane (1.5 litres). The hexane extract was further washed with brine, dried over sodium sulphate, and filtered through a short plug of silica (approx. 80 g), eluting with 1:1 hexane:dichloromethane (500 ml). Evaporation afforded an oil (36.8 g, 74%).
MS (+ve ion electrospray) m/z 192 (MH+).
A solution of 7-fluoro-8-methyl-2-(methyloxy)quinoline (36.7 g, 192 mmol) in trifluoromethylbenzene (500 ml) was treated with N-bromosuccinimide (37.6 g, 211 mmol) and benzoyl peroxide (243 mg, 1 mmol) and heated at 70° C. while irradiating with a 120 Watt tungsten lamp for 1 hour. The cooled mixture was filtered, washed with dichloromethane, and the combined organic fractions were washed with saturated aqueous sodium bicarbonate solution then dried. The solution was filtered through a plug of silica and evaporated affording a pale yellow solid (51.4 g, 99%).
MS (+ve ion electrospray) m/z 271 (MH+).
A solution of 8-(bromomethyl)-7-fluoro-2-(methyloxy)quinoline (18.25 g; 67.6 mmol) in dimethoxyethane (340 ml) and water (51 ml) was degassed with a stream of argon for 10 minutes, then potassium carbonate (8.8 g), [1,1′-bis(diphenylphosphino)ferrocene]dichloro-palladium(II): DCM complex (1.12 g; 1.53 mmol) and 2,4,6-trivinylcyclotriboroxane pyridine complex (14.7 g) were added and the mixture was heated under reflux overnight. More 2,4,6-trivinylcyclotriboroxane pyridine complex (7.3 g) was added and heating was continued overnight. The mixture was evaporated to dryness and azeotroped with dry toluene and the residue was chromatographed on silica gel, eluting with DCM, then 5% ethyl acetate/hexane gave impure product (about 50% pure by LC-MS and NMR) (5.5 g).
LC-MS (+ve ion electrospray) m/z 218 (MH+).
Further elution with methanol gave a 1-{[7-fluoro-2-(methyloxy)-8-quinolinyl]methyl}pyridinium species [MS (+ve ion electrospray) m/z 269 (MH+) (12 g), which on treatment with 2,4,6-trivinylcyclotriboroxane pyridine complex (as above, for 72 hours followed by evaporation, treatment with sodium bicarbonate and extraction with chloroform) gave further product (3.4 g; ca. 80% pure by LC-MS), after chromatography on silica gel (eluting with 2% methanol-DCM).
Impure 7-fluoro-2-(methyloxy)-8-(2-propen-1-yl)quinoline (ca. 50% pure; 4.1 g) in 2-butanol (50 ml) and water (50 ml) was stirred with AD mix a/p (1:1 mixture) (28.4 g) at room temperature for 72 hours. Water was added to dissolve the solid and the clear top layer (butanol) was separated and washed with a little water. The combined aqueous layers were extracted with 10% methanol-chloroform, dried (sodium sulphate), added to the butanol layer and evaporated. The product was chromatographed on silica gel, eluting with 0-15% methanol-DCM, to afford the product (3.0 g) as an oil (88% pure by LC-MS).
LC-MS (+ve ion electrospray) m/z 252 (MH+).
(2R/S)-3-[7-Fluoro-2-(methyloxy)-8-quinolinyl]-1,2-propanediol (2.18 g; 8.7 mmol)) in DCM (30 ml) and triethylamine (1.22 ml) was treated with tosyl chloride (1.82 g; 9.6 mmol) and dibutyltin(IV) oxide (108 mg; 5 mol. %), and the mixture was stirred at room temperature for 4.5 hr. Further tosyl chloride (150 mg) was added and the mixture was stirred for a further 30 minutes, then sodium bicarbonate solution was added and the mixture was extracted (3×) with DCM, dried (sodium sulphate) and evaporated to dryness to give the product, which was used immediately in the next step.
LC-MS (+ve ion electrospray) m/z 406 (MH+).
(2R/S)-3-[7-Fluoro-2-(methyloxy)-8-quinolinyl]-2-hydroxypropyl 4-methylbenzenesulfonate (assume 8.7 mmol) and 1,1-dimethylethyl 4-piperidinylcarbamate (7.0 g, 35 mmol) in ethanol/DMF (5 ml/1 ml) and disodium hydrogenphosphate (5.0 g, 35 mmol) were heated under reflux for 18 hours and cooled. Water and sodium bicarbonate was added and the mixture was extracted (3×) with DCM, dried (sodium sulphate), evaporated and chromatographed on silica gel, eluting with 0-100% ethyl acetate-hexane to afford the product as an oil (ca. 75% pure, 1.05 g).
LC-MS (+ve ion electrospray) m/z 434 (MH+).
A solution of (2R/S)-1,1-dimethylethyl (1-{3-[7-fluoro-2-(methyloxy)-8-quinolinyl]-2-hydroxypropyl}-4-piperidinyl)carbamate (1.1 g; ca. 75% pure) (2.54 mmol), methanesulfonic anhydride (0.575 g; 3.3 mmol) and diisopropylethylamine (0.86 ml; 5.1 mmol) was heated at 88° C. for 3 hours and cooled. Sodium carbonate solution was added and the mixture was extracted with chloroform and then DCM (×2), dried (sodium sulphate), evaporated and chromatographed on silica gel, eluting with 0-100% ethyl acetate-hexane to afford the product. Several impure fractions were re-chromatographed, as above, to give further material. (Total yield 0.58 g).
LC-MS (+ve ion electrospray) m/z 402 (MH+).
(10) (2R/S)-9-Fluoro-2-(hydroxymethyl)-1,2-dihydro-4H-pyrrolo[3,2,1-ij]quinolin-4-one
A solution of 7-fluoro-2-(methyloxy)-8-(2-propen-1-yl)quinoline (ca. 50% pure, 2.0 g; ca. 4.6 mmol) in chloroform was treated with m-chloroperbenzoic acid (ca. 60% pure with water; 1.6 g) and the solution was stirred at room temperature overnight. More m-chloroperbenzoic acid (0.8 g) was added and the solution was stirred at room temperature for 72 hours. MP Carbonate resin (12 g) and excess solid sodium metabisulphite was added and the mixture was stirred at room temperature for 3 hours. It was filtered, evaporated to one third volume and chromatographed on silica gel, eluting with 0-2% methanol-DCM, then 1:1 ethyl acetate-DCM to afford a 57:25 mixture (0.85 g) of 9-fluoro-2-(hydroxymethyl)-1,2-dihydro-4H-pyrrolo[3,2,1-ij]quinolin-4-one (less polar, longer retention time on a reverse phase LC-MS system) and an isomer, believed to be 10-fluoro-2-hydroxy-2,3-dihydro-1H,5H-pyrido[3,2,1-ij]quinolin-5-one (more polar, shorter retention time on a reverse phase LC-MS system).
LC-MS (+ve ion electrospray) m/z 220 (MH+).
A 57:25 mixture (80% pure; 0.75 g; 3.42 mmol) of (2R/S)-9-fluoro-2-(hydroxymethyl)-1,2-dihydro-4H-pyrrolo[3,2,1-ij]quinolin-4-one and 10-fluoro-2-hydroxy-2,3-dihydro-1H,5H-pyrido[3,2,1-ij]quinolin-5-one in dry DCM (40 ml) was stirred with 1,1,1-tris-(acetyloxy)-1,1-dihydro-1,2-benziodooxol-3-(1H)-one (Dess-Martin periodinane) (1.74 g; 4.1 mmol), solid sodium bicarbonate (2.87 g; 3.4 mmol) and 3A molecular sieves at room temperature for 2 hours. Methanol (5 drops) was added and the mixture was filtered, evaporated and divided into two equal portions. One portion was chromatographed on silica gel, eluting with 0-5% methanol-DCM, to afford a fraction (70 mg) containing impure 9-fluoro-4-oxo-1,2-dihydro-4H-pyrrolo[3,2,1-ij]quinoline-2-carbaldehyde (70 mg).
MS (+ve ion electrospray) m/z 218 (MH+).
δH (CDCl3), (400 MHz) 9.95 (1H, s, CHO)
Impure 9-fluoro-4-oxo-1,2-dihydro-4H-pyrrolo[3,2,1-ij]quinoline-2-carbaldehyde (assume 200 mg 0.922 mmol), and 1,1-dimethylethyl 4-piperidinylcarbamate (370 mg; 1.85 mmol) were heated in chloroform (3 ml) and dry methanol (3 ml) under reflux with 3A molecular sieves for 1 hour. The mixture was cooled and sodium triacetoxyborohydride (586 mg; 2.76 mmol) was added and the mixture was stirred at room temperature for 72 hours. Sodium carbonate solution was added and the mixture was extracted (3×) with DCM, dried (sodium sulphate), evaporated and chromatographed on silica gel, eluting with 0-15% methanol-DCM, to give an oil (27 mg).
MS (+ve ion electrospray) m/z 402 (MH+).
A solution of (2R/S)-1,1-dimethylethyl {1-[(9-fluoro-4-oxo-1,2-dihydro-4H-pyrrolo[3,2,1-ij]quinolin-2-yl)methyl]-4-piperidinyl}carbamate (0.70 g) in dry DCM (10 ml) and dry methanol (10 ml) was treated with 4M hydrogen chloride in 1,4-dioxane (15 ml) and the solution was stirred for 3 hours at room temperature, then evaporated to dryness and azeotroped with toluene/methanol. It was dissolved in methanol (10 ml) and DCM (10 ml) and stirred with excess MP-carbonate resin (7.0 g) at room temperature overnight, filtered, and evaporated to give a solid (760 mg) (contains some inorganics/resin). (95% pure by LC-MS).
LC-MS (+ve ion electrospray) m/z 302 (MH+).
A solution of (2R/S)-[(4-amino-1-piperidinyl)methyl]-9-fluoro-1,2-dihydro-4H-pyrrolo[3,2,1-ij]quinolin-4-one (ca. 67% pure) (110 mg, assume 73 mg pure material, 0.242 mmol) and 6,7-dihydro[1,4]dioxino[2,3-c]pyridazine-3-carbaldehyde (for a preparation see Example 3(e)) (46 mg, 0.28 mmol) in chloroform/methanol (3 ml/3 ml) was stirred at room temperature under argon overnight with excess 3A molecular sieves. The mixture was treated with sodium triacetoxyborohydride (154 mg, 0.7 mmol) and stirred for 5 hours at room temperature. Further 6,7-dihydro[1,4]dioxino[2,3-c]pyridazine-3-carbaldehyde (15 mg) was added and the mixture was stirred at room temperature overnight. The mixture was treated with saturated aqueous sodium carbonate solution and 10% methanol in chloroform and the phases separated. The aqueous phase was further extracted (3×) with 10% methanol in chloroform. The combined organic extracts were dried (sodium sulphate) and evaporated. Chromatography eluting with 0-15% methanol in DCM afforded the free bases of the title compounds as the racemate (50 mg).
LC-MS (+ve ion electrospray) m/z 452 (MH+).
δH (CDCl3), (400 MHz) 1.30-1.50 (2H, m), 1.75-1.95 (2H, m), 2.15-2.30 (2H, m), 2.40-2.60 (2H, m), 2.67 (1H, d), 3.05 (1H, d), 3.11 (1H, dd), 3.40-3.55 (3H, m), 4.00 (2H, s), 4.38 (2H, m), 4.51 (2H, m), 5.08 (1H, m), 6.55 (1H, d), 6.88 (1H, t), 7.05 (1H, s), 7.35 (1H, m), 7.65 (1H, d)
LC-MS (+ve ion electrospray) m/z 452 (MH+).
The racemate free base in methanol/chloroform was treated with excess 4M HCl in 1,4-dioxane, evaporated, and the resulting solid washed with ether to give the title compounds (dihydrochloride) as a racemic mixture. LC-MS (+ve ion electrospray) m/z 452 (MH+).
Racemic title compound dihydrochloride (54 mg) was separated by chiral preparative HPLC on a Chiralpak AS-H column (21×250 mm L) eluting with 90:10:0.1 acetonitrile/methanol/isopropylamine mixture at 20 ml/min and monitoring by UV at 254 nM, to provide Enantiomer 1 (free base), 22 mg (>99% ee; retention time 6.1 min) and Enantiomer 2 (free base), 19 mg (>99% ee; retention time 12.8 min).
The free bases were converted to mono hydrochloride salts by dissolving in methanol, treatment with 1 equivalent of 6.0 M HCl, and evaporating to dryness.
To a solution of 2-bromo-3-fluoroaniline (5.29 g, 27.8 mmol) in acetone (12 ml) was added potassium carbonate (5.75 g, 41.8 mmol) followed by water (15 ml) and the stirred mixture cooled to 0° C. Cinnamoyl chloride (4.63 g, 27.8 mmol) was added portionwise over 15 min then the mixture stirred for a further 2 h. After this time the reaction mixture was poured onto ice/water and the resulting precipitate collected and dried to yield a white solid (8.1 g).
LC-MS: m/z 320/322 (MH+)+.
(b) 8-Bromo-7-fluoro-2(1H)-quinolinone To a stirred suspension of N-(2-bromo-3-fluorophenyl)-3-phenyl-2-propenamide (8.0 g, 25 mmol) in chlorobenzene (40 ml) was added aluminium chloride (20.0 g, 150 mmol) portionwise over about 5 min. The mixture was then heated to 120° C. for 1.5 h cooled to ˜50° C. and added slowly onto ice. A pink oil/solid separated out and the mixture was allowed to stand overnight. The mixture was extracted with ethyl acetate and the organics dried and concentrated to a reddish solid (4.9 g) which was largely the title compound. The solids were washed with hexane and dried to yield a pink solid (2.35 g).
LC-MS: m/z 242/244 (MH+)+.
To a solution of 8-bromo-7-fluoro-2(1H)-quinolinone (2.0 g, 8.3 mmol) in DMF (30 ml) was added potassium carbonate (2.28 g, 16.6 mmol) followed by methyl iodide (11.0 ml, 9.9 mmol) and the mixture stirred for 3 h. The mixture was separated between ethyl acetate and water and the organics isolated, dried and concentrated to provide a dark solid (2.1 g).
LC-MS: m/z 257 (MH+)+.
To a solution of 8-bromo-7-fluoro-2-(methyloxy)quinoline (1.51 g, 5.9 mmol) in 1,4-dioxane (20 ml) was added allyltributylstannane (1.9 ml, 5.9 mmol) followed by cesium fluoride (1.9 g, 12.9 mmol). The mixture was degassed thoroughly then tris(dibenzylideneacetone)dipalladium(0) (0.06 g, 0.06 mmol) and bis(tri-t-butylphosphine)palladium(0) (0.06 g, 0.12 mmol) added and the mixture degassed again. The mixture was heated at 75° C. for 3 h then allowed to cool and separated between ethyl acetate and brine. The organics were isolated, dried and concentrated to a red oil. Chromatography (10 g silica SPE, gradient elution hexane/dichloromethane 0% to 15%) provided the title compound contaminated with some tin residues (1.72 g).
LC-MS: m/z 218 (MH+)+.
To a solution of 7-fluoro-2-(methyloxy)-8-(2-propen-1-yl)quinoline (0.5 g, 2.3 mmol) in DMF (5 ml) was added water (0.3 ml) followed by NBS (0.41 g, 2.3 mmol) and the solution stirred for 2 h. The reaction mixture was separated between ethyl acetate and brine and the organics isolated, washed with brine, dried and concentrated. Chromatography of the residues (70 g silica SPE, gradient elution with dichloromethane/hexane 2:1 to dichloromethane then dichloromethane/ethyl acetate 1:0 to 4:1) to yield a mixture of 2-(bromomethyl)-9-fluoro-1,2-dihydro-4H-pyrrolo[3,2,1-ij]quinolin-4-one and 2-bromo-10-fluoro-2,3-dihydro-1H,5H-pyrido[3,2,1-ij]quinolin-5-one in a 4:1 ratio (0.27 g).
LC-MS: m/z 282/284 (MH+)+.
To a solution of 2-(bromomethyl)-9-fluoro-1,2-dihydro-4H-pyrrolo[3,2,1-ij]quinolin-4-one and 2-bromo-10-fluoro-2,3-dihydro-1H,5H-pyrido[3,2,1-ij]quinolin-5-one in a 4:1 ratio (0.25 g, 0.9 mmol) in acetonitrile (20 ml) was added 1,1-dimethylethyl 4-piperidinylcarbamate (0.18 g, 0.9 mmol) and potassium carbonate (0.124 g, 0.9 mmol). The mixture was heated at 50° C. for 24 h whereupon lc/ms monitoring of the reaction showed only 50% conversion. Therefore a further equivalent of 1,1-dimethylethyl 4-piperidinylcarbamate (0.18 g, 0.9 mmol) was added together with potassium carbonate (0.124 g, 0.9 mmol) and heated for a further 24 h at 50° C. followed by heating at 70° C. for 2 h. After cooling the solution was concentrated and the residues separated between ethyl acetate and water. The organics were separated, dried and concentrated. Chromatography of the residues (20 g silica SPE, gradient elution with hexane/ethyl acetate 40%-100%) provided a yellow solid (0.15 g).
LC-MS: m/z 402 (MH+)+.
To a solution of 1,1-dimethylethyl (1-{[(2R/S)-9-fluoro-4-oxo-1,2-dihydro-4H-pyrrolo[3,2,1-ij]quinolin-2-yl]methyl}-4-piperidinyl}carbamate (0.14 g, 0.35 mmol) in dichloromethane (1 ml) was added HCl in 1,4dioxane (1 ml, 4M solution) followed by methanol (0.5 ml). The solution was stirred for 3 h then concentrated to provide a cream solid (0.14 g).
LC-MS: m/z 302 (MH+)+.
To a suspension of (2R/S)-2-[(4-amino-1-piperidinyl)methyl]-9-fluoro-1,2-dihydro-4H-pyrrolo[3,2,1-ij]quinolin-4-one hydrochloride (0.12 g, 0.32 mmol) in dichloromethane (5 ml) was added triethylamine (0.11 ml, 0.8 mmol) whereupon all the solids dissolved. To the solution was added 2,3-dihydro[1,4]dioxino[2,3-c]pyridine-7-carbaldehyde (for a synthesis see WO2004058144, Example 2(c) or WO03/087098, Example 19(d)) (0.053 g, 0.32 mmol) followed by sodium triacetoxyborohydride (0.20 g, 0.96 mmol) and the solution stirred for 4 h. The reaction mixture was separated between dichloromethane and saturated sodium bicarbonate solution. The organics were separated and washed with brine, dried (MgSO4) and concentrated to provide the free bases of the title compounds (racemate) as a clear oil (0.14 g, 100%).
δH (CDCl3), (400 MHz) 1.30-1.50 (2H, m), 1.75-1.95 (2H, m), 2.15-2.30 (2H, m), 2.40-2.55 (2H, m), 2.65 (1H, d), 3.05 (1H, d), 3.11 (1H, dd), 3.40-3.55 (3H, m), 3.75 (2H, s), 4.25 (2H, m), 4.35 (2H, m), 5.08 (1H, m), 6.55 (1H, d), 6.8 (1H, s), 6.85 (1H, t), 7.35 (1H, m), 7.65 (1H, d), 8.1 (1H, s).
LC-MS: m/z 451 (MH+)+.
Chromatography of a portion (80 mg) of this material on a Chiralpak AS-H column eluting with 90:10:0.1 acetonitrile:methanol:isopropylamine afforded 19 mg of the free base of Enantiomer 1 isomer (retention time 5.0 minute), followed by 23 mg of the free base of Enantiomer 2 isomer (retention time 13.2 minutes).
The free bases were converted to hydrochloride salts by dissolving in methanol, treatment with 1 equivalent of 6.0 M HCl, and evaporating to dryness.
A solution of 3,4,6-trichloropyridazine (25 g) in tetrahydrofuran (200 ml) and triethylamine (19 ml) was treated at 0° C. (ice bath cooling) with 2-mercaptoethanol (8.33 ml) over 5 minutes. After the addition was complete, the mixture was stirred at room temperature for 72 hours. The mixture was stirred with aqueous sodium bicarbonate solution and dichloromethane and the solid was collected, washed with water, ether and pentane and dried in vacuo, giving (22.9 g). The combined aqueous and organic fraction was evaporated to half volume giving further solid, which was washed and dried as above (5.0 g). The total yield of solid (27.9 g; 91%) contained some bromo-derivative (5-10%) by NMR.
A solution of 2-[(3,6-dichloro-4-pyridazinyl)thio]ethanol (13 g) (previously dried at 50° C. in vacuo) in dry 1,4-dioxane (250 ml) was treated with lithium hydride (3 g) in portions and heated at 105-110° C. for 24 hours. The reaction mixture was cooled and quenched with iced-water. The solution was taken to pH 10-11 with 5M hydrochloric acid and evaporated. Water was added and the mixture was extracted 4× with dichloromethane, dried (sodium sulphate), evaporated, and chromatographed on silica gel, eluting with 0-100% ethyl acetate-hexane, to afford a white solid (1.61 g) (containing ca. 10% of a bromo species).
MS (+ve ion electrospray) m/z 189/91 (Cl MH+); 233/5 (Br MH+)
δH (CDCl3, 400 MHz) 3.23 (2H, m), 4.67 (2H, m), 7.26 (1H, s) (for major chloro-compound).
A solution of 3-chloro-6,7-dihydro[1,4]oxathiino[2,3-c]pyridazine (1.0 g) in dimethoxyethane (25 ml) was degassed under argon then tetrakis(triphenylphosphine)palladium (0) (135 mg), potassium carbonate (0.695 g), triethenylboroxin pyridine complex (0.8 g) and water (3.7 ml) were added. The mixture was heated overnight at 105° C. More triethenylboroxin pyridine complex (0.4 g) and tetrakis(triphenylphosphine)palladium (0) (30 mg) were added and heating was continued for 24 hours. The mixture was cooled, treated with aqueous sodium bicarbonate solution, extracted (4×) with dichloromethane, dried (sodium sulphate), evaporated and chromatographed on silica gel (70 g), eluting with 0-100% ethyl acetate-hexane, affording a solid (0.56 g) (87% pure by LC-MS).
MS (+ve ion electrospray) m/z 181 (MH+).
A solution of 3-ethenyl-6,7-dihydro[1,4]oxathiino[2,3-c]pyridazine (320 mg) in 1,4-dioxane/water (20 ml/5 ml) was treated with an aqueous solution of osmium tetroxide (4% w/v, 2 ml) and sodium periodate (1.08 g), initially stirred in an ice-bath, then allowed to warm to room temperature. After 2.5 hours the mixture was evaporated to dryness and dissolved in 1,4-dioxane and chloroform. Silica gel was added and the mixture was evaporated to dryness, added to a silica column (50 g) and chromatographed, eluting with 0-100% ethyl acetate in hexane, to afford a white solid (116 mg, 36%).
MS (+ve ion electrospray) m/z 183 (MH+).
A solution of (2R/S)-[(4-amino-1-piperidinyl)methyl]-9-fluoro-1,2-dihydro-4H-pyrrolo[3,2,1-ij]quinolin-4-one (for a preparation see Example 12(b)) (ca. 67% pure) (110 mg, assume 73 mg pure material; 0.242 mmol) and 6,7-dihydro[1,4]oxathiino[2,3-c]pyridazine-3-carbaldehyde (ca. 80% pure) (55 mg, assume 44 mg pure material; 0.243 mmol) in methanol (2 ml) and chloroform (2 ml) with 3A sieves was stirred overnight. Sodium triacetoxyborohydride (150 mg, 0.70 mmol) was added and stirred for 8 hours. Further sodium triacetoxyborohydride was added and stirring was continued overnight. The mixture was basified with sodium carbonate solution and extracted with 10% methanol in DCM (3×), and the extracts were dried and evaporated. Chromatography on silica gel eluting with 0-10% methanol in DCM gave the racemic free base (29 mg).
δH (CDCl3), (250 MHz) 1.30-1.50 (2H, m), 1.55-1.95 (2H, m), 2.10-2.35 (2H, m), 2.35-2.60 (2H, m), 2.70 (1H, d), 3.00-3.30 (4H, m), 3.35-3.60 (2H, m), 3.97 (2H, s), 4.60-4.75 (2H, m), 5.10 (1H, m), 6.57 (1H, d), 6.89 (1H, t), 7.34 (2H, m), 7.66 (1H, d).
The enantiomers were separated by chiral preparative HPLC on a Chiralpak AS-H column (21×250 mm L) eluting with 90:10:0.1 acetonitrile/methanol/isopropylamine mixture at 20 ml/min and monitoring by UV at 254 nM. 24 mg of racemate was processed to provide Enantiomer 1 (7 mg, >99% ee; retention time 3.9 min) and Enantiomer 2 (7 mg, >99% ee; retention time 10.9 min).
The free bases were dissolved in methanol and treated with one equivalent of 6.0 M HCl, and evaporated to give the title compounds (each 8 mg).
MS (+ve ion electrospray) m/z 468 (MH+).
A solution of chloroacetyl chloride (38.4 ml, 480 mmol) in ethyl acetate (100 ml) was added to a vigorously-stirred mixture of 3-fluoro-2-methylaniline (60 g, 480 mmol), saturated aq. sodium bicarbonate (400 ml), ice (ca 100 g) and ethyl acetate (400 ml). After 1 hour the mixture was concentrated on a rotary evaporator (removing most of the ethyl acetate) and filtered, washing with water. The resulting white solid was dried in vacuo (94.4 g, 98%).
LC-MS (+ve ion electrospray): m/z 202/204 [MH+]
A solution of 2-chloro-N-(3-fluoro-2-methylphenyl)acetamide (47.1 g, 243 mmol) in conc. sulphuric acid (220 ml) was treated at ca. −20° C. (ethanol/water/CO2 bath) with conc. nitric acid (69%, 15.2 ml, 19 g, 235 mmol) over 30 minutes, keeping internal temperature <10° C. The reaction was complete at the end of the addition. The mixture was added to ice with vigorous stirring then left to stand overnight. Filtration, washing of the solid with water and drying afforded the product (54.6 g, 95%).
LC-MS (−ve ion electrospray): m/z 245/247 [(MH+)−]
2-Chloro-N-(3-fluoro-2-methyl-6-nitrophenyl)acetamide (prepared from 47.2 g of 2-chloro-N-(3-fluoro-2-methylphenyl)acetamide, ca. 234 mmol) was suspended in water (220 ml) then treated with aqueous sodium hydroxide solution (12.5M, 110 ml, 1.37 mol). Tetrahydrofuran (110 ml) was added, then the mixture was heated to reflux for 5 hours, then concentrated on a rotary evaporator (removing most of the tetrahydrofuran). The yellow solid was filtered off and washed with cold water until washing were non-alkaline. Drying in vacuo afforded a yellow solid (32.3 g, 81% over 2 steps).
LC-MS (+ve ion electrospray): m/z 171 [MH+]
To a solution of 3-fluoro-2-methyl-6-nitroaniline (10.0 g, 58.8 mmol) and cyanoacetic acid (10.1 g, 117.5 mmol) in dry toluene (500 ml) was added phosphorus pentachloride (25.8 g, 117.5 mmol) portionwise with stirring. The mixture was then heated at 120-125° C. while passing air over the mixture. After 2.5 h, an extra 1 g of cyanoacetic acid and 2.6 g phosphorus pentachloride were added and heating continued for 1 h. After cooling, toluene was evaporated and the residue was dissolved in ethyl acetate and washed with brine, brine/sodium bicarbonate (twice) and brine, dried and evaporated. The crude product was recrystallised from ethanol, washed with ethanol/hexane (1:1) and dried to give 10.72 g (77%), which contained some 6-fluoro-5-methyl-3-oxo-3,4-dihydro-2-quinoxalinecarbonitrile 1-oxide (lower-running yellow spot on TLC, 1:1 ethyl acetate/hexane).
LC-MS (−ve ion electrospray): m/z 236 [(M-H+)−]
2-Cyano-N-(3-fluoro-2-methyl-6-nitrophenyl)acetamide (11.17 g, 45 mmol) was partially dissolved in pyridine (50 ml) and 1M aq. sodium hydroxide (46 ml) was added, giving a clear solution. The mixture was stirred at room temperature for 24 h, then water was added to dissolve all solids and the mixture was filtered, washing through with water. The filtrate (˜300 ml) was acidified with 5M HCl to pH6. The precipitate was filtered off, washed with water and dried to give 8.13 g of product. The filtrate was acidified to pH1 and extracted three times with ethyl acetate. The extracts were dried and evaporated and the residue was azeotroped with toluene to give 2.28 g product containing some of the acetamide starting material.
Total yield 10.41 g, containing approx. 7% acetamide.
LC-MS (+ve ion electrospray): m/z 220 [MH+]
A mixture of 6-fluoro-5-methyl-3-oxo-3,4-dihydro-2-quinoxalinecarbonitrile 1-oxide (15.9 g, 71.8 mmol) and sodium dithionite (36.7 g, 179.6 mmol) in ethanol (200 ml) and water (400 ml) was heated under reflux for 1 h, with a flow of argon over the top of the condenser leading to a bleach-filled Drechsel bottle to trap HCN. The cooled mixture was carefully acidified to pH1 with 5M HCl and the mixture was stirred for 45 min at room temperature. Sodium hydroxide (50% aq.) was then added to give pH11-12 and the mixture was evaporated to remove approx. 500 ml. The residue was acidified to pH 6 with 5M HCl (caution: cyanide still present!) and the precipitate was filtered off, washed with water and dried to give a solid (10.37 g, 81%). Extraction of the aqueous liquor with 10% methanol/dichloromethane and evaporation of the extracts gave a further small amount of product (0.8 g). Total yield 11.2 g, 88%.
LC-MS (+ve ion electrospray): m/z 179 [MH+]
7-Fluoro-8-methyl-2(1H)-quinoxalinone (5.75 g, 32.3 mmol) in phosphorus oxychloride (30 ml) was heated under reflux for 2 h. Phosphorus oxychloride was removed by evaporation, and the residue was basified with aq. sodium bicarbonate and extracted several times with ethyl acetate. The extracts were dried and evaporated, and the residue was dissolved in dichloromethane and passed quickly through a short column of silica (20 g), washing through with more dichloromethane. Removal of solvent gave a light brown solid (4.13 g, 65%).
LC-MS (+ve ion electrospray): m/z 197/199 [MH+]
2-Chloro-7-fluoro-8-methylquinoxaline (4.1 g, 20.9 mmol) was suspended in dry methanol and a 25% solution of sodium methoxide in methanol (4.73 ml, 21.9 mmol) was added. The mixture was heated under reflux for 2 h, then evaporated. The residue was dissolved in dichloromethane and water and the phases were separated. The aqueous phase was extracted twice with dichloromethane, and combined organic fractions were dried and evaporated to give a light brown solid (3.84 g, 96%).
LC-MS (+ve ion electrospray): m/z 193 [MH+]
7-Fluoro-8-methyl-2-(methyloxy)quinoxaline (50 g, 260 mmol) was dissolved in dry chloroform (IL). N-Bromosuccinimide (52 g, 292 mmol) and benzoyl peroxide (70%, 0.42 g) were added and the mixture was heated under reflux, illuminated with a 120 W lamp, for 2 h. After cooling, the mixture was washed with water. The aqueous phase was re-extracted twice with dichloromethane and the combined organic fractions were washed with water, dried and evaporated to give an off-white solid (72.1 g, 100%).
LC-MS (+ve ion electrospray): m/z 271/273 [MH+]
A solution of 8-bromomethyl-7-fluoro-2-(methyloxy)quinoxaline (25 g, 92.2 mmol) in 1,4-dioxane (450 mL) and water (60 mL) was flushed with argon, then 2,4,6-trivinylcyclotriboroxane pyridine complex (15 g, 62.1 mmol), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) dichloromethane complex (1.52 g) and potassium carbonate (12 g, 185 mmol) were added. The mixture was heated under reflux, under argon, for approx. 72 h. After cooling, water and ethyl acetate were added and the phases were separated. The aqueous phase was extracted twice with ethyl acetate and the combined organic fractions were dried and evaporated. Chromatography on silica, eluting with 0-20% ether/hexane gave the product (8.23 g, 41%), containing a few mole percent of the 8-methyl analogue.
LC-MS (+ve ion electrospray): m/z 219 [MH+]
To a solution of 7-fluoro-2-(methyloxy)-8-(2-propen-1-yl)quinoxaline (8.23 g, 37.75 mmol), in tert-butanol (250 mL) and water (250 mL) was added a 1:1 mixture of AD mix alpha and beta (50 g). The mixture was stirred overnight at room temperature, another portion of 10 g of AD mix alpha and beta was added and stirring was continued for three days. Sodium sulphite (60 g) was added and the mixture was stirred for 30 min., then the phases were separated. The aqueous phase was extracted twice with 10% methanol/dichloromethane and the combined organic fractions were dried and evaporated to give the product (9.57 g, 100%).
LC-MS (+ve ion electrospray): m/z 253 [MH+]
(2R/S)-3-[6-Fluoro-3-(methyloxy)-5-quinoxalinyl]-1,2-propanediol (9.57 g, 37.75 mmol) was partially dissolved in anhydrous tetrahydrofuran/dichloromethane (1:1, 400 mL). Triethylamine (7.85 mL, 56.6 mmol) and dibutyltin(IV) oxide (0.47 g, 1.9 mmol) were added, followed by 4-methylbenzensulfonyl chloride (7.16 g, 37.75 mmol). The mixture was stirred at room temperature overnight, then aqueous sodium bicarbonate was added and the phases were separated. The aqueous phase was extracted with dichloromethane and the organic fractions were washed with brine, dried and evaporated to give the product (15.4 g, approx. 100%), containing some residual triethylamine and a few percent of 7-fluoro-2-(methyloxy)-8-(2-oxiranylmethyl)quinoxaline.
LC-MS (+ve ion electrospray): m/z 407 [MH+]
A mixture of (2R/S)-3-[6-fluoro-3-(methyloxy)-5-quinoxalinyl]-2-hydroxypropyl 4-methylbenzenesulfonate (15.4 g, 37.75 mmol), 1,1-dimethylethyl 4-piperidinylcarbamate (15.1 g, 75.5 mmol) and sodium carbonate (12.0 g, 113.25 mmol) in ethanol (180 mL) and dimethylformamide (45 mL) was heated at 40° C. for approx. 64 h. The mixture was evaporated and the residue was dissolved in brine and ethyl acetate. The phases were separated and the aqueous phase was extracted twice with ethyl acetate. Organic fractions were dried and evaporated, and the residue was chromatographed on silica, eluting with 0-20% methanol/ethyl acetate to give the product (8.05 g, 49%).
LC-MS (+ve ion electrospray): m/z 435 [MH+]
To a solution of 1,1-dimethylethyl (1-{(2R/S)-3-[6-fluoro-3-(methyloxy)-5-quinoxalinyl]-2-hydroxypropyl}-4-piperidinyl)carbamate (7.02 g, 16.15 mmol) in dry chloroform (80 mL) was added methanesulfonic anhydride (3.37 g, 18.5 mmol) and di-isopropylethylamine (6.32 mL, 36.2 mmol). The mixture was stirred for 0.5 h at room temperature, and then heated under reflux for 24 h. After cooling, water was added and the phases were separated. The aqueous phase was extracted twice with dichloromethane, and the organic fractions were dried and evaporated. Chromatography on silica eluted with 50-100% ethyl acetate/hexane gave the title isomeric products 1,1-dimethylethyl (1-{[(5R/S)-7-fluoro-3-oxo-5,6-dihydro-3H-pyrrolo[1,2,3-de]quinoxalin-5-yl]methyl}-4-piperidinyl)carbamate (eluted first, 3.55 g, 55%), and 1,1-dimethylethyl {1-[(6R/S)-8-fluoro-3-oxo-6,7-dihydro-3H,5H-pyrido[1,2,3-de]quinoxalin-6-yl]-4-piperidinyl}carbamate (eluted second, 0.84 g, 13%).
LC-MS (+ve ion electrospray): m/z 403 [MH+]
To a solution of 1,1-dimethylethyl (1-{[(5R/S)-7-fluoro-3-oxo-5,6-dihydro-3H-pyrrolo[1,2,3-de]quinoxalin-5-yl]methyl}-4-piperidinyl)carbamate (0.21 g, 0.52 mmol) in dichloromethane (4 mL) and methanol (2.5 mL) was added hydrogen chloride in 1,4-dioxane (4M, 4 mL). The mixture was stirred for 1.75 h then evaporated to dryness. The residue was dissolved in 10% methanol/dichloromethane (20 mL) and stirred with MP-carbonate resin (0.77 g, 2.69 mmol/g) for 1 h. The resin was filtered off, washed a few times with 10% methanol/dichloromethane and methanol alternately, and the filtrate was evaporated to give the free amine (0.19 g, approx. 83% pure).
LC-MS (+ve ion electrospray): m/z 303 [MH+]
(5R/S)-5-[(4-Amino-1-piperidinyl)methyl]-7-fluoro-5,6-dihydro-3H-pyrrolo[1,2,3-de]quinoxalin-3-one (approx. 83% pure, 0.19 g, 0.52 mmol) and 6,7-dihydro[1,4]dioxino[2,3-c]pyridazine-3-carbaldehyde (for a preparation see Example 3(e)) (96 mg, 0.58 mmol) were dissolved in dry chloroform and methanol (1:1, 14 mL) and heated under reflux overnight with 3A molecular sieves. After cooling, sodium triacetoxyborohydride (0.44 g, 2.08 mmol) was added and the mixture was stirred for 8 h at room temperature. Aqueous sodium bicarbonate was added to basify and the phases were separated. The aqueous phase was extracted several times with dichloromethane and 10% methanol/dichloromethane, and the organic fractions were dried and evaporated. Chromatography on silica, eluting with 0-10% methanol/dichloromethane gave the title compounds as free bases (0.144 g, 61%).
δH (CDCl3, 250 MHz) 1.38 (2H, m), 1.88 (2H, m), 2.24 (2H, m), 2.50 (2H, m), 2.69 (1H, br. d), 3.00 (1H, br. d), 3.13 (1H, dd), 3.48 (1H, m, part. obscured by solvent)), 3.99 (2H, s), 4.37 (2H, m), 4.51 (2H, m), 5.08 (1H, m), 6.99 (1H, t), 7.02 (1H, s), 7.65 (1H, dd), 8.15 (1H, s)
LC-MS (+ve ion electrospray): m/z 453 [MH+]
The free bases in dichloromethane/methanol were treated with 0.4M hydrogen chloride in 1,4-dioxane (1.6 mL), and the solvent was evaporated to give the racemic dihydrochloride salt (167 mg).
Racemic 5-({4-[(6,7-dihydro[1,4]dioxino[2,3-c]pyridazin-3-ylmethyl)amino]-1-piperidinyl}methyl)-7-fluoro-5,6-dihydro-3H-pyrrolo[1,2,3-de]quinoxalin-3-one dihydrochloride (148 mg) was separated by preparative HPLC on a 21×250 mm Chiralpak AS-H® column, 2 injections, eluting with acetonitrile/methanol/isopropylamine (90/10/0.1, 20 ml/min, UV monitoring at 254 nm) to give the two enantiomers E1 (retention time 3.6 min.) and E2 (retention time 5.1 min.) of the free base of the title compounds.
The separate enantiomers in methanol were both treated with one equivalent of aqueous hydrochloric acid, and the solvent was evaporated to give the title hydrochloride salts.
A solution of the free base (0.84 g) in dichloromethane/methanol was treated with a solution of fumaric acid (216 mg) in methanol. The mixture was evaporated and the resulting solid triturated with ether and dried (1.05 g).
The free base (29 mg) was purified on a reverse phase C18 column eluting with aqueous acetonitrile containing 1% TFA. Removal of solvents afforded a solid (30 mg).
A solution of the free base (1.1 g) in dichloromethane/methanol was treated with a solution of fumaric acid (281 mg) in methanol. The mixture was evaporated and the resulting solid triturated with ether and dried (1.24 g).
The free base (900 mg) was dissolved in methanol (50 ml) and treated with benzoic acid (243 mg) then evaporated to dryness affording an off-white solid (1.17 g).
1,1-Dimethylethyl {1-[(6R/S)-8-fluoro-3-oxo-6,7-dihydro-3H,5H-pyrido[1,2,3-de]quinoxalin-6-yl]-4-piperidinyl}carbamate (0.88 g, 2.19 mmol) was dissolved in dichloromethane (16 mL) and methanol (10 mL) and treated with 4M hydrogen chloride I 1,4-dioxane (16 mL). After stirring for 1.5 h at room temperature, the mixture was evaporated. The residue was partially dissolved in 10% methanol/dichloromethane (50 mL) and stirred with MP-carbonate resin (2.69 mmol/g, 3.27 g) for approx. 2 h. The resin was filtered off, washed several times with 10% methanol/dichloromethane and methanol alternately, and the filtrate was evaporated. The crude product was chromatographed on silica, eluting with 20-50% methanol/dichloromethane to give the amine (0.51 g, 77%).
LC-MS (+ve ion electrospray): m/z 302 [MH+]
A solution of (6R/S)-6-(4-amino-1-piperidinyl)-8-fluoro-6,7-dihydro-3H,5H-pyrido[1,2,3-de]quinoxalin-3-one (0.10 g, 0.33 mmol) and 2,3-dihydro[1,4]dioxino[2,3-c]pyridine-7-carboxaldehyde (for a synthesis see WO2004058144, Example 2(c) or WO03/087098, Example 19(d)) (55 mg, 0.33 mmol) in dry 1:1 chloroform/methanol (10 ml) was heated under reflux with 3A molecular sieves overnight. After cooling, sodium triacetoxyborohydride (0.28 g, 1.32 mmol) was added and the mixture was stirred at room temperature for 8 h, then allowed to stand overnight. Aqueous sodium bicarbonate was added to basify, and the phases were separated. The aqueous phase was extracted three times with 10% methanol/dichloromethane, and the organic fractions were dried and evaporated. Chromatography on silica, eluting with 0-20% methanol/dichloromethane, gave the free base (131 mg, 88%).
δH (CDCl3, 250 MHz) 1.44 (2H, m), 1.92 (2H, m), 2.39 (2H, m), 2.53 (1H, m), 2.70-3.10 (4H, complex m), 3.22 (1H, d), 3.63 (1H, dd), 3.81 (2H, s), 4.30 (4H, m), 4.65 (1H, d), 6.82 (1H, s), 7.05 (1H, t), 7.70 (1H, dd), 8.11 (1H, s), 8.23 (1H, s).
LC-MS (+ve ion electrospray): m/z 452 [MH+]
The free base (31 mg) in dichloromethane/methanol was treated with excess 0.4M hydrogen chloride in 1,4-dioxane (0.34 mL), and the solvent was evaporated to give the title dihydrochloride salt (35 mg). The racemic material (100 mg) was partially resolved on Chiralpak IA using acetonitrile:THF:isopropylamine. The enriched second eluting enantiomer (E2) was then further resolved on Chiralpak IA using CH3OH:CH3CN:isopropanol:isopropylamine to give 28 mg of free base which was converted to the mono hydrochloride salt (30 mg) with 1 equivalent of hydrochloric acid.
A solution of (6R/S)-6-(4-amino-1-piperidinyl)-8-fluoro-6,7-dihydro-3H,5H-pyrido[1,2,3-de]quinoxalin-3-one (for a preparation see Example 20) (0.10 g, 0.33 mmol) and 6,7-dihydro[1,4]dioxino[2,3-c]pyridazine-3-carbaldehyde (for a preparation see Example 3(e)) (55 mg, 0.33 mmol) in dry 1:1 chloroform/methanol (10 ml) was heated under reflux with 3A molecular sieves overnight. After cooling, sodium triacetoxyborohydride (0.28 g, 1.32 mmol) was added and the mixture was stirred at room temperature for 8 h, then allowed to stand overnight. Aqueous sodium bicarbonate was added to basify, and the phases were separated. The aqueous phase was extracted three times with 10% methanol/dichloromethane, and the organic fractions were dried and evaporated. Chromatography on silica, eluting with 0-20% methanol/dichloromethane, gave the free base (118 mg, 79%).
δH (CDCl3, 250 MHz) 1.41 (2H, m), 1.94 (2H, br. d), 2.38 (2H, q), 2.55 (1H, m), 2.70-3.10 (4H, complex m), 3.21 (1H, d), 3.67 (1H, dd), 4.01 (2H, s), 4.37 (2H, m), 4.51 (2H, m), 4.62 (1H, d), 7.04 (1H, s) overlapping 7.05 (1H, t), 7.70 (1H, dd), 8.23 (1H, s).
LC-MS (+ve ion electrospray): m/z 453 [MH+]
The free base (28 mg) in dichloromethane/methanol was treated with 0.4M hydrogen chloride in 1,4-dioxane (0.31 mL), and the solvent was evaporated to give the dihydrochloride salt (33 mg).
Racemic 6-{4-[(6,7-Dihydro[1,4]dioxino[2,3-c]pyridazin-3-ylmethyl)amino]-1-piperidinyl}-8-fluoro-6,7-dihydro-3H,5H-pyrido[1,2,3-de]quinoxalin-3-one (90 mg) was separated by preparative HPLC on a 21×250 mm Chiralpak AS-H® column, 4 injections, eluting with acetonitrile/methanol/isopropylamine (98/2/0.1, 20 ml/min, UV monitoring at 254 nm) to give the two enantiomers E1 (retention time 6.9 min.) and E2 (retention time 7.5 min.).
The separate isomers in methanol were both treated with one equivalent of aqueous hydrochloric acid, and the solvent was evaporated to give the hydrochloride salts.
A solution of (5R/S)-5-[(4-amino-1-piperidinyl)methyl]-7-fluoro-5,6-dihydro-3H-pyrrolo[1,2,3-de]quinoxalin-3-one (for a preparation see Example 18(o)) (0.30 g, 1 mmol) in dry methanol (5 mL) was treated with sodium methoxide (25% in methanol, 0.65 mL, 3 mmol) and heated under reflux overnight. The cooled mixture was treated with aqueous ammonium chloride (6 drops) and evaporated to dryness. The residue was extracted three times with 10% methanol/dichloromethane, the extracts were filtered and evaporated. The crude product was chromatographed on silica, eluting with 0-20% (2M ammonia in methanol)/dichloromethane to give the amine (0.20 g, 64%).
LC-MS (+ve ion electrospray): m/z 315 [MH+]
A solution of (5R/S)-5-[(4-amino-1-piperidinyl)methyl]-7-(methyloxy)-5,6-dihydro-3H-pyrrolo[1,2,3-de]quinoxalin-3-one (0.20 g, 0.64 mmol) and 6,7-dihydro[1,4]dioxino[2,3-c]pyridazine-3-carbaldehyde (for a preparation see Example 3(e)) (116 mg, 0.70 mmol) in dry 1:1 chloroform/methanol (16 ml) was heated under reflux with 3A molecular sieves overnight. After cooling, sodium triacetoxyborohydride (0.54 g, 2.55 mmol) was added and the mixture was stirred at room temperature overnight. Aqueous sodium bicarbonate was added to basify, and the phases were separated. The aqueous phase was extracted several times with 10% methanol/dichloromethane, and the organic fractions were dried and evaporated. Chromatography on silica, eluting with 0-20% methanol/dichloromethane, gave the free base (185 mg, 62%).
δH (CDCl3, 250 MHz) 1.40 (2H, m), 1.88 (2H, m, part. obscured by water), 2.24 (2H, m), 2.48 (1H, m), 2.52 (1H, m), 2.72 (1H, br. d), 3.02 (1H, br. d), 3.17 (1H, dd), 3.39 (1H, m), 3.96 (3H, s), 4.00 (2H, s), 4.38 (2H, m), 4.51 (2H, m), 5.04 (1H, m), 6.87 (1H, d), 7.03 (1H, s), 7.63 (1H, d), 8.05 (1H, s).
LC-MS (+ve ion electrospray): m/z 465 [MH+]
The free base in chloroform/dichloromethane/methanol was treated with excess 0.4M hydrogen chloride in 1,4-dioxane (1.99 mL), and the solvent was evaporated to give the dihydrochloride salt (213 mg).
A solution of 1,1-dimethylethyl {1-[(5R/S)-3-fluoro-8-oxo-5,6-dihydro-4H,8H-pyrido[3,2,1-de]-1,5-naphthyridin-5-yl]-4-piperidinyl}carbamate (for a preparation see Example 1(e)) (35 mg, 0.09 mmol) was dissolved in dichloromethane and trifluoroacetic acid (1 ml/1 ml) and stirred for 1 hour then evaporated. The residue was dissolved in dichloromethane/methanol and treated with MP-carbonate resin until a moistened pH paper indicated pH 8. The mixture was filtered, washing with methanol and evaporated affording an oil. Analysis indicated a 4:1 mixture of the anticipated (5R/S)-5-(4-amino-1-piperidinyl)-3-fluoro-5,6-dihydro-4H,8H-pyrido[3,2,1-de]-1,5-naphthyridin-8-one and 3-fluoro-6H,8H-pyrido[3,2,1-de]-1,5-naphthyridin-8-one. This material was dissolved in chloroform/methanol (1 ml/0.1 ml) and treated with 2,3-dihydro[1,4]dioxino[2,3-c]pyridine-7-carboxaldehyde (for a synthesis see WO2004058144, Example 2(c) or WO03/087098, Example 19(d)) (14 mg, 0.09 mmol) and sodium triacetoxyborohydride (55 mg, 0.3 mmol). After 2 hours the mixture was added to a silica column and chromatographed eluting with 0-30% methanol in dichloromethane affording the monoacetate salt of the free base of the title compound as an oil (30 mg).
δH (CD3OD, 250 MHz) 1.50-1.70 (2H, m), 1.95 (3H, s), 2.10-2.30 (2H, m), 2.35-2.50 (2H, m), 3.05-3.25 (4H, m), 4.05-4.20 (3H, m), 4.25-4.40 (7H, m), 6.85 (1H, d), 7.00 (1H, s), 7.95 (1H, d), 8.10 (1H, s), 8.45 (1H, s)
This material was treated with excess hydrochloric acid in methanol and evaporated to afford the dihydrochloride salt (ca 30 mg).
MS (+ve ion electrospray) m/z 452 (MH+).
A solution of (5R/S)-5-[(4-amino-1-piperidinyl)methyl]-3-fluoro-4,5-dihydro-7H-pyrrolo[3,2,1-de]-1,5-naphthyridin-7-one (for a preparation see Example 1(f)) (332 mg, 1.1 mmol) in methanol (25 ml) was treated with sodium methoxide (25% solution in methanol, 1.0 ml, 4.6 mmol) and heated to 60° C. for 6 hours. More sodium methoxide (25% solution in methanol, 1.0 ml, 4.6 mmol) was added and the mixture heated at 60° C. for 17 hours. The mixture was cooled in an ice bath and taken to ca pH8 with saturated aqueous ammonium chloride then evaporated to dryness. The residue was suspended in methanol and added to an SCX column, washing off with 2M methanolic ammonia. Evaporation afforded an orange gum (389 mg,).
MS (ES+) m/z 315 (MH+).
A solution of 5-[(4-amino-1-piperidinyl)methyl]-3-(methyloxy)-4,5-dihydro-7H-pyrrolo[3,2,1-de]-1,5-naphthyridin-7-one (170 mg) and 2,3-dihydro[1,4]dioxino[2,3-c]pyridine-7-carboxaldehyde (for a synthesis see WO2004058144, Example 2(c) or WO03/087098, Example 19(d)) (89 mg) in dichloromethane/methanol (10 ml/2 ml) was treated with sodium triacetoxyborohydride (343 mg, 0.52 mmol) and stirred for 20 hours then treated with saturated aqueous sodium bicarbonate solution. The phases were separated and the aqueous phase further extracted three times with 10% methanol in dichloromethane. The combined extracts were washed with brine, dried and evaporated. Chromatography eluting with 0-30% methanol in dichloromethane afforded the free base of the title compounds as a yellow gum (121 mg). The free base (20 mg) was dissolved in chloroform (0.33 ml) and treated with 1M hydrochloric acid in ether (0.5 ml) followed by ether (1 ml). Removal of solvent gave the product (23 mg).
1H NMR (400 MHz) δ (CDCl3) 1.30-1.45 (2H, m), 1.75-1.95 (2H, m), 2.18 (1H, t), 2.25 (1H, t), 2.50 (2H, m), 2.65 (1H, d), 3.05 (1H, d), 3.15 (1H, dd), 3.45 (1H, dd), 3.55 (1H, dd), 3.80 (2H, s), 4.08 (3H, s), 4.30 (2H, m), 4.38 (2H, m), 5.05 (1H, m), 6.68 (1H, d), 6.80 (1H, s), 7.82 (1H, d), 8.10 (1H, s), 8.22 (1H, s),
MS (ES+) m/z 464 (MH+).
Chromatography of a portion (78 mg) of this material (free base) on a Chiralpak AS-H column eluting with a water/acetonitrile gradient (1% isopropylamine present) afforded separate enantiomers (Rt Enantiomer 1, 2.4 minutes, alphaD+133 (c=0.5 in methanol), Rt Enantiomer 2, 3.9 minutes, alphaD−143 (c=0.9 in methanol)). Each material was then converted to the separate title compounds with one equivalent of hydrochloric acid (25 mg E1 and 28 mg E2).
(a) 6-(Methyloxy)-4-(2-propen-1-yl)-1,5-naphthyridine-3-carbonitrile A solution of 4-bromo-6-(methyloxy)-1,5-naphthyridine-3-carbonitrile (prepared from 4-bromo-6-(methyloxy)-1,5-naphthyridine-3-carboxylic acid (WO2004058144, Example 53(d)) via conversion to 4-bromo-6-(methyloxy)-1,5-naphthyridine-3-carboxamide with oxalyl bromide then ammonia and then treatment of 4-bromo-6-(methyloxy)-1,5-naphthyridine-3-carboxamide with trifluoromethane sulfonic anhydride and triethylamine to give 4-bromo-6-(methyloxy)-1,5-naphthyridine-3-carbonitrile) (200 mg, 0.76 mmol) in degassed 1,4-dioxane (2 ml) was treated with tris(dibenzylideneacetone)dipalladium(0), (6.9 mg, 0.008 mmol), bis(tri-tert-butylphosphine)palladium(0) (7.8 mg, 0.015 mmol), allyl tributylstannane (0.2 ml, 0.76 mmol) and cesium fluoride (253 mg, 1.7 mmol). The reaction mixture was heated at 70° C. under argon for 2 hours, cooled and partitioned between water and ethyl acetate. The aqueous was extracted further with ethyl acetate and the combined organic phases were dried, evaporated and chromatographed (15% ethyl acetate/hexane) to afford the desired compound (156 mg; 92%).
MS (ES+) m/z 226 (MH+).
A solution of 6-(methyloxy)-4-(2-propen-1-yl)-1,5-naphthyridine-3-carbonitrile (60 mg, 0.0.27 mmol) in 1:1 THF:water (1 ml) was treated with sodium chlorate (43 mg, 0.4 mmol) and then osmium tetroxide (0.01 ml, 4% aqueous solution, 0.00134 mmol) The reaction mixture was stirred for 6 h, then evaporated, treated with acetonitrile, sonicated for 5 minutes and then filtered. The filtrate was evaporated and the residue chromatographed (80% ethyl acetate/hexane) to afford the desired compound as a white solid (39 mg; 56%).
MS (ES+) m/z 260 (MH+).
A solution of 4-(2,3-dihydroxypropyl)-6-(methyloxy)-1,5-naphthyridine-3-carbonitrile (3 g, 11.6 mmol) in DCM (150 ml) at 0° C. under argon was treated with triethylamine (3.22 ml, 23.2 mmol) and para-toluenesulfonic anhydride (5.28 g, 16.2 mmol) and stirred at 0° C. for 1 hour. Ethanol was added and the reaction stirred for a further 0.5 hour, reaction was evaporated, treated with ethanol and evaporated again. The residue was suspended in ethanol (100 ml) and treated with 1,1-dimethylethyl 4-piperidinylcarbamate (4.6 g, 23 mmol) and sodium carbonate (3.87 g, 34.8 mmol) and then stirred at 60° C. under argon for 24 hours. The reaction was then partitioned between water and DCM, and the aqueous phase extracted twice more with DCM. The combined organic phases were dried, evaporated and chromatographed (99:1:0.1 DCM:Methanol:0.88 ammonia) to afford the desired compound (2.92 g; 57%).
MS (ES+) m/z 442 (MH+).
A solution of 1,1-dimethylethyl (1-{3-[3-cyano-6-(methyloxy)-1,5-naphthyridin-4-yl]-2-hydroxypropyl}-4-piperidinyl)carbamate (2.15 g, 4.87 mmol) in DCM (40 ml) at 0° C. under argon was treated with sodium carbonate (2.12 g, 20 mmol) and methanesulfonic anhydride (1.74 g, 10 mmol) and stirred for 4 hours. More sodium carbonate (2.12 g, 20 mmol) and methanesulfonic anhydride (1.74 g, 10 mmol) were added and the reaction was allowed to stir overnight. The solvent was then evaporated, then the residue dissolved in chloroform (40 ml) and treated with sodium carbonate (2.12 g, 20 mmol) and methanesulfonic anhydride (1.74 g, 10 mmol). The reaction mixture was stirred for 6 hours, then partitioned between water and DCM, and the aqueous phase separated twice more with DCM. The combined organic phases were dried, evaporated and chromatographed (98:2:0.2 DCM:methanol:0.88 ammonia) to afford a mixture of the desired compound and starting material (1.7 g). Some of this material (1.26 g) was treated with sodium carbonate (6.36 g) and methanesulfonic anhydride (5.22 g) in DCM (20 ml) at 0° C. under argon and stirred for 4 hours and then worked up again as above and purified by MDAP (Mass Directed Auto Purification) to give the desired product (65 mg).
MS (ES+) m/z 410 (MH+).
A solution of 1,1-dimethylethyl 1′-[(3-cyano-7-oxo-4,5-dihydro-7H-pyrrolo[3,2,1-de]-1,5-naphthyridin-4-yl)methyl]-4-piperidinyl}carbamate (84 mg) in DCM (4 ml) was treated with trifluoroacetic acid and stirred for 1 hour. The reaction mixture was then evaporated to dryness then redissolved and passed through a column of Amberlyst A-21 basic resin, eluting with methanol. The fractions containing the desired compound were then evaporated and chromatographed (99:1:0.1 DCM:Methanol:0.88 ammonia) to afford the desired compound (41 mg; 64%).
MS (ES+) m/z 310 (MH+).
A solution of 4-[(4-amino-1-piperidinyl)methyl]-7-oxo-4,5-dihydro-7H-pyrrolo[3,2,1-de]-1,5-naphthyridine-3-carbonitrile (41 mg, 0.113 mmol) and 2,3-dihydro[1,4]dioxino[2,3-c]pyridine-7-carboxaldehyde (22 mg, 0.113 mmol)(for a synthesis see WO2004058144, Example 2(c) or WO03/087098, Example 19(d)) in 1:1 methanol:chloroform (2 ml) with 3A molecular sieves was heated at 65° C. for 4 hours, cooled in ice and treated with sodium triacetoxyborohydride (56 mg, 0.266 mmol) and stirred overnight. The reaction was then partitioned between water and 20% methanol:DCM, and the aqueous phase extracted twice more with 20% methanol:DCM. The combined organic phases were dried, evaporated and purified by MDAP (Mass Directed Auto Purification), evaporated and triturated with diethyl ether to afford the desired compound (40 mg; 54%).
1H NMR (400 MHz) δ (mixture of CD3OD:CDCl3) 1.69 (m, 2H), 2.05 (m, 2H), 2.30 (m, 2H), 2.60 (m, 1H), 2.80 (m, 1H), 3.0 (m, 1H), 3.15 (m, 2H), 3.72 (m, 2H), 4.12 (s, 2H), 4.48 (m, 4H), 5.12 (m, 1H), 7.01 (m, 2H), 8.02 (d, 1H), 8.30 (s, 1H), 8.41 (br s, 2H), 8.71 (s, 1H)
MS (ES+) m/z 459 (MH+).
A mixture of (5R/S)-5-[(4-amino-1-piperidinyl)methyl]-7-fluoro-5,6-dihydro-3H-pyrrolo[1,2,3-de]quinoxalin-3-one (for a preparation see Example 18(o)) (200 mg, 0.66 mmol), 2,3-dihydro[1,4]dioxino[2,3-c]pyridine-7-carboxaldehyde (for a synthesis see WO2004058144, Example 2(c) or WO03/087098, Example 19(d)) (130 mg, 0.8 mmol), triethylamine (0.2 ml), in methanol/chloroform (10 ml/10 ml) was heated to reflux overnight in the presence of 3A molecular sieves. The mixture was treated at room temperature with sodium triacetoxyborohydride (367 mg) and stirred for 7 hours then treated with saturated aqueous sodium bicarbonate solution. The phases were separated and the aqueous phase further extracted several times with 10% methanol in dichloromethane. The combined extracts were dried and evaporated affording a yellow solid (320 mg). Chromatography eluting with 0-20% methanol in dichloromethane afforded the free base of the title compounds as a yellow gum (87 mg, 30%).
1H NMR (250 MHz, CDCl3) 1.42 (2H, m), 1.89 (2H, br t), 2.10-2.35 (2H, m), 2.50 (2H, m), 2.70 (1H, d), 3.01 (1H, dd), 3.12 (1H, dd), 3.49 (2H, m), 3.80 (2H, s), 4.30 (4H, m), 5.08 (1H, m), 6.82 (1H, s), 6.99 (1H, t), 7.65 (1H, dd), 8.10 (1H, s), 8.15 (1H, s)
MS (ES+) m/z 452 (MH+).
This material was converted to the corresponding fumarate (98 mg) by adding 1 equivalent of fumaric acid to a dichloromethane solution of the free base then evaporating. Chromatography of a portion (70 mg) of this material on a Chiralpak AS-H column eluting with a water/acetonitrile gradient (1% TFA present) afforded separate enantiomers (Rt Enantiomer 1, 5.6 min minutes; Rt Enantiomer 2, 10.6 minutes, baseline separation). Each material was then converted to the separate title compounds with one equivalent of benzoic acid (24 mg E1 and 23 mg E2).
A mixture of (5R/S)-5-[(4-amino-1-piperidinyl)methyl]-7-fluoro-5,6-dihydro-3H-pyrrolo[1,2,3-de]quinoxalin-3-one (for a preparation see Example 18(o)) (50 mg, 0.17 mmol) and 3-oxo-3,4-dihydro-2H-pyrido[3,2-b][1,4]oxazine-6-carboxaldehyde (for a synthesis, see WO2004058144, Example 1(1)) (35 mg, 0.2 mmol) in chloroform/methanol (3 ml/3 ml) was heated under reflux with 3A molecular sieves overnight. The mixture was treated at room temperature with sodium triacetoxyborohydride (110 mg, 0.52 mmol) and stirred for 24 hours then treated with saturated aqueous sodium bicarbonate solution. The phases were separated and the aqueous phase further extracted three times with 10% methanol in dichloromethane. The combined extracts were dried and evaporated. Chromatography eluting with 0-20% methanol in dichloromethane afforded the free base of the title compounds as a yellow gum (55 mg, 71%).
1H NMR (250 MHz, CDCl3) 1.45 (2H, m), 1.89 (2H, br t), 2.15-2.35 (2H, m), 2.52 (2H, m), 2.72 (1H, br d), 3.02 (1H, br d), 3.15 (1H, dd), 3.48 (2H, m), 3.84 (2H, s), 4.64 (2H, s), 5.07 (1H, m), 6.98 (2H, m), 7.20 (1H, d), 7.65 (1H, dd), 8.15 (1H, s)
MS (ES+) m/z 465 (MH+)
This material was converted to the corresponding dihydrochloride (59 mg) by adding 2 equivalents of hydrochloric acid in 1,4-dioxane to a chloroform/dichloromethane solution of the free base then evaporating. Chromatography of a portion (30 mg) of this material on a Chiralpak AS-H column eluting with 85:15:0.1 acetonitrile:methanol:isopropylamine afforded separate enantiomers (Rt Enantiomer 1, 5.5 min minutes; Rt Enantiomer 2, 7.0 minutes), giving 9.5 mg E1 and 9.5 mg E2.
(6R/S)-6-(4-Amino-1-piperidinyl)-8-fluoro-6,7-dihydro-3H,5H-pyrido[1,2,3-de]quinoxalin-3-one (for a preparation see Example 20(a)) (500 mg) was chromatographed on a Chiralpak AS-H column eluting with 85:15:0.1 acetonitrile:methanol:isopropylamine afforded separate enantiomers (Rt Enantiomer 1, 5.3 minutes; Rt Enantiomer 2, 13.6 minutes), giving 189 mg E1 and 159 mg E2.
A mixture of 6-(4-amino-1-piperidinyl)-8-fluoro-6,7-dihydro-3H,5H-pyrido[1,2,3-de]quinoxalin-3-one, Enantiomer E1 (100 mg, 0.33 mmol) and 3-oxo-3,4-dihydro-2H-pyrido[3,2-b][1,4]oxazine-6-carboxaldehyde (for a synthesis, see WO2004058144, Example 1(1)) (60 mg, 0.33 mmol) in chloroform/methanol (5 ml/5 ml) with 3A molecular sieves and acetic acid (16 drops) was stirred at room temperature for 1 hour then sodium cyanoborohydride (84 mg) was added. After 3 hours saturated aqueous sodium bicarbonate solution was added. The phases were separated and the aqueous phase further extracted with 10% methanol in dichloromethane. The combined extracts were dried and evaporated. Chromatography eluting with 0-20% methanol in dichloromethane afforded a yellow gum (40 mg). This material was converted to the dihydrochloride salt by treatment with 2 equivalents of hydrochloric acid in 1,4-dioxane (40 mg). This material was dissolved in dichloromethane/methanol (1 ml/6 ml) and treated with MP-carbonate resin followed by manganese(II) oxide. After 2 hours the mixture was filtered and evaporated to give the free base of the title compound (28 mg). This material was converted to the dihydrochloride salt by treating a solution of the free base with 2 equivalents of HCl in 1,4-dioxane followed by evaporation affording the title compound as an off-white solid (25 mg).
1H NMR (di-HCl salt) (250 MHz, d6-DMSO) 2.19 (2H, br), 2.38 (2H, br), 3.65 (2H, br), 3.96 (2H, br), 4.18 (2H, s), 4.71 (2H, s), 4.89 (1H, br), 7.24 (1H, d), 7.30 (1H, t), 7.46 (1H, d), 7.81 (1H, m), 8.25 (1H, s), 9.59 (2H, br).
MS (ES+) m/z 465 (MH+)
This was prepared from 6-(4-amino-1-piperidinyl)-8-fluoro-6,7-dihydro-3H,5H-pyrido[1,2,3-de]quinoxalin-3-one, Enantiomer E2 by the procedure of Example 32, yielding the title product as an off-white solid (32 mg).
1H NMR (di-HCl salt) (250 MHz, CD3OD) 1.72 (2H, br), 2.22 (2H, br), 2.58 (2H, br), 3.11 (2H, m), 3.69 (2H, m), 4.07 (1H, m), 4.26 (2H, s), 4.44 (1H, d), 4.70 (2H, s), 7.09 (1H, d), 7.18 (1H, t), 7.37 (1H, d), 7.75 (1H, m), 8.18 (1H, s).
MS (ES+) m/z 465 (MH+)
To a solution of dimethyl malonate (2.5 g, 18.9 mmol) in anhydrous THF (20 mL) was added NaH (0.038 g, 0.95 mmol, 60% in mineral oil). The reaction was stirred at ambient temperature for 15 minutes. In a separate flask, ethyl acrylate (1.02 mL, 9.45 mmol) was dissolved in anhydrous THF (1 mL) and then added dropwise over 30 minutes to the dimethyl malonate solution. The reaction was stirred at ambient temperature overnight and then concentrated under vacuum. The residue was dissolved in EtOAc, washed with saturated NH4Cl solution and brine. The organic phase was dried over Na2SO4, filtered, and concentrated under vacuum. The crude residue was purified by column chromatography (silica gel) using an EtOAc/hexanes gradient to yield the desired compound (1.68 g, 77%).
1H NMR (400 MHz, CDCl3) δ 1.24 (t, J=7.07 Hz, 3H) 2.20 (q, J=7.24 Hz, 2H) 2.37 (t, J=7.33 Hz, 2H) 3.47 (t, J=7.33 Hz, 1H) 3.70-3.75 (m, 6H) 4.12 (q, J=7.24 Hz, 2H).
Cinnamonitrile (25.0 g, 194 mmol) was dissolved in EtOH. The solution was cooled to 0° C. and HCl gas bubbled through the solution for 30 minutes. The solution was stirred at ambient temperature for 1 h and then concentrated under vacuum. The residue was dissolved in EtOH (100 mL), cooled to 0° C. and a solution of NH3/MeOH (7M, 69 mL, 484 mmol) was added dropwise through an addition funnel. Once added, the solution was allowed to warm to ambient temperature and stirred overnight and the resulting NH4Cl was filtered off. The solution was concentrated under vacuum and the resulting product was used without further purification (28.6 g crude).
LC-MS: m/z 147.4 (MH+).
3-Ethyl 1,1-dimethyl 1,1,3-propanetricarboxylate (1.65 g, 7.11 mmol) and (2E)-3-phenyl-2-propenimidamide (1.04 g, 7.11 mmol) were combined in EtOH (36 mL). Triethylamine (1.98 mL, 14.2 mmol) was added and the solution was heated at reflux for 3 h with no change based on LC-MS. The solution was cooled to room temperature and treated with NaOMe in MeOH (1.0 mL, 5.33 mmol, 25-30% w/w solution) and the solution was refluxed for 3 h. Another two portions of NaOMe in MeOH (2×1.0 mL) were added and the solution was refluxed overnight. After this time, a yellow precipitate had formed which was filtered off. The mother liquor was acidified to pH 2 with 1N HCl, and the solution was concentrated under vacuum. The resulting material was combined with the yellow solid and used without further purification.
LC-MS: m/z 315.2 (MH+).
Crude ethyl 3-{4-hydroxy-6-oxo-2-[(E)-2-phenylethenyl]-1,6-dihydro-5-pyrimidinyl}propanoate (7.1 mmol) was dissolved in POCl3 (25 mL) and N,N-dimethylaniline (0.862 g, 0.9 mL, 7.1 mmol) was slowly added to the solution. The reaction was then heated at reflux for 2 h. After cooling to ambient temperature, the resulting solution was carefully and slowly added to ice water to quench the excess POCl3. The mixture was extracted with EtOAc (3×) and concentrated under vacuum. The crude residue was then purified by column chromatography (silica gel) using an EtOAc/hexanes gradient to yield the desired compound as a yellow solid (0.48 g, 19% over 2 steps).
LC-MS: m/z 351.4 (MH+).
To a solution of ethyl 3-{4,6-dichloro-2-[(E)-2-phenylethenyl]-5-pyrimidinyl}propanoate (0.42 g, 1.19 mmol) in 1,4-dioxane (5 mL) was added conc. NH4OH (3.5 mL). The reaction was heated at 75° C. in a sealed tube overnight. The solution was concentrated under vacuum, diluted with water, and extracted with EtOAc/DCM. The organic layer was washed with brine, dried over Na2SO4, and concentrated under vacuum. The crude residue was then purified by column chromatography (silica gel) to yield the desired compound (0.072 g, 21%).
LC-MS: m/z 286.2 (MH+).
Also obtained was 3-{4-amino-6-chloro-2-[(E)-2-phenylethenyl]-5-pyrimidinyl}propanamide (0.175 g).
LC-MS: m/z 303.3 (MH+).
3-{4-Amino-6-chloro-2-[(E)-2-phenylethenyl]-5-pyrimidinyl}propanamide (0.175 g, 0.58 mmol) was dissolved in EtOH and HCl gas was bubbled through the solution until saturated. The solution was heated at reflux for 2 h, cooled to ambient temperature and concentrated under vacuum. The residue was dissolved in water, neutralised to pH9 with K2CO3 solution and extracted with EtOAc (3×). The organic layers were combined, dried over Na2SO4, filtered and concentrated under vacuum to yield ethyl 3-{4-amino-6-chloro-2-[(E)-2-phenylethenyl]-5-pyrimidinyl}propanoate as a white solid. LC-MS: m/z 332.2 (MH+). This product was then dissolved in DMF (5 mL), treated with K2CO3 (0.16 g, 1.16 mmol) and heated at 75° C. for 30 minutes. The solution was cooled, diluted with water and extracted with Et2O (3×). The organic layer was dried over Na2SO4, filtered, and concentrated under vacuum. The crude residue was then purified by column chromatography (silica gel) with DCM/(DCM:MeOH:NH4OH) 90:10:1 to yield an additional 0.11 g of the desired compound.
LC-MS: m/z 286.2 (MH+).
4-Chloro-2-[(E)-2-phenylethenyl]-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one (0.18 g, 0.64 mmol) was dissolved in a 2:1 solution of 1,4-dioxane/water (6 mL) and cooled to 0° C. NaIO4 (0.314 g, 1.47 mmol) and catalytic OSO4 (1 mL, 4% aq. solution) were added and the solution was then stirred at ambient temperature overnight. The reaction solution was concentrated under vacuum, diluted with water, and extracted with 10% MeOH/DCM (4×). The organic layers were combined, dried over Na2SO4, filtered and concentrated under vacuum. The crude residue was then purified by column chromatography (silica gel) using a DCM/DCM-MeOH—NH4OH (90:10:1) gradient to yield the desired compound (0.05 g, 44%).
LC-MS: m/z 212.0 (MH+).
To a solution of 4-chloro-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidine-2-carbaldehyde (1.43 g, 6.78 mmol) in MeOH was added p-TsOH.H2O (0.13 g, 0.68 mmol). The solution was heated at reflux for 2.5 h and then cooled to ambient temperature. The solution was concentrated under vacuum to yield the desired product which was used without further purification.
LC-MS: m/z 257.9 (MH+).
To crude 2-[bis(methyloxy)methyl]-4-chloro-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one (presumed 6.78 mmol) dissolved in MeOH was added 10% Pd/C (0.15 g). The solution was stirred under an atmosphere of H2 (balloon) overnight. The Pd/C was filtered off and the solution concentrated under vacuum. The crude residue was purified by column chromatography (silica gel) using a DCM/DCM-MeOH—NH4OH (90:10:1) gradient to yield the desired product as a white solid (0.873 g, 58% over 2 steps).
LC-MS: m/z 223.9 (MH+).
To a solution of 2-[bis(methyloxy)methyl]-5,8-dihydropyrido[2,3-d]pyrimidin-7(6H)-one (0.873 g, 3.91 mmol) in 1:1 H2O/acetone (10 mL) was added p-TsOH.H2O (0.074 g, 0.391 mmol) and the reaction was heated to 80° C. for 3 days with additional p-TsOH.H2O (0.20 g). After the disappearance of starting material, the solution was concentrated under vacuum to yield the desired product (1.023 g).
LC-MS: m/z 178.0 (MH+).
To a solution of 5-[(4-amino-1-piperidinyl)methyl]-7-fluoro-5,6-dihydro-3H-pyrrolo[1,2,3-de]quinoxalin-3-one (for a preparation see Example 18(o)) (60 mg, 0.2 mmol) in methanol/DCM (10 ml/10 ml), was added 7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidine-2-carbaldehyde (50 mg; 0.2 mmol), and excess solid sodium sulphate. The solution was stirred at ambient temperature for 16 hours, followed by addition of sodium triacetoxyborohydride (0.126 g; 0.6 mmol). The resulting solution was stirred for 2 hours. The solution was then concentrated onto silica gel under vacuum and the crude residue purified by column chromatography on silica gel (CH2Cl2/90:10:1 CH2Cl2/MeOH/NH4OH) to yield the free base of the desired product as a yellowish oily film (0.033 g, 36%).
1H NMR 6 (400 MHz, CDCl3) 1.55 (d, 2H) 1.88-2.04 (m, 2H), 2.14-2.37 (m, 2H) 2.53 (dd, 1H), 2.61-2.81 (m, 4H), 2.98 (t, 2H), 3.06 (d, 1H), 3.17 (dd, 1H), 3.51 (d, 2H), 4.05 (s, 2H), 5.09 (dd, 1H), 5.31 (s, 1H), 6.99 (t, 1H), 7.66 (dd, 1H), 8.16 (s, 1H) 8.39 (s, 1H).
MS (ES+) m/z 464.2 (MH+).
The free base of the title compound dissolved in DCM was treated with one equivalent 1M hydrogen chloride in diethyl ether and evaporated to dryness to give the title compound as a light tan solid. (31 mg).
A solution of 5-[(4-amino-1-piperidinyl)methyl]-3-(methyloxy)-4,5-dihydro-7H-pyrrolo[3,2,1-de]-1,5-naphthyridin-7-one (for a preparation see Example 25(a)) (170 mg, 0.54 mmol) and 6,7-dihydro[1,4]dioxino[2,3-c]pyridazine-3-carbaldehyde (for a preparation see Example 3(e)) (100 mg, 0.60 mmol) in dichloromethane/methanol (10 ml/2 ml) was treated with sodium triacetoxyborohydride (343 mg, 0.52 mmol) and stirred for 18 hours. More aldehyde (20 mg) and more sodium triacetoxyborohydride (100 mg) were added. After 6 hours the mixture was treated with saturated aqueous sodium bicarbonate solution and dichloromethane. The phases were separated and the aqueous phase further extracted with dichloromethane. The combined extracts were washed with brine, dried and evaporated. Chromatography eluting with 0-30% methanol in dichloromethane afforded partially purified material which was triturated with ether then dried in vacuo affording the free base of the title compound as a yellow gum (56 mg).
1H NMR (250 MHz) 6 (CDCl3) 1.30-1.45 (2H, m), 1.75-1.95 (2H, m), 2.20 (1H, t), 2.28 (1H, t), 2.45-2.55 (2H, m), 2.65 (1H, d), 3.05 (1H, d), 3.15 (1H, dd), 3.45 (1H, dd), 3.55 (1H, dd), 3.98 (2H, s), 4.07 (3H, s), 4.35 (2H, m), 4.52 (2H, m), 5.05 (1H, m), 6.68 (1H, d), 7.03 (1H, s), 7.85 (1H, d), 8.10 (1H, s), 8.25 (1H, s),
MS (ES+) m/z 465 (MH+).
The free base of the title compound was dissolved in 2.5 ml of 19:1 chloroform:methanol and treated with 1M hydrochloric acid in ether (1 ml). More ether (4 ml) was added and the resulting suspension was centrifuged. Decantation of the supernatant and drying of the residue afforded the title compound (70 mg) as a solid.
Whole-cell antimicrobial activity was determined by broth microdilution using the Clinical and Laboratory Standards Institute (CLSI) recommended procedure, Document M7-A7, “Methods for Dilution Susceptibility Tests for Bacteria that Grow Aerobically”. The compounds were tested in serial two-fold dilutions ranging from 0.016 to 16 mcg/mL.
Compounds were evaluated against Gram-positive organisms, selected from Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Enterococcus faecalis and Enterococcus faecium.
In addition, compounds were evaluated against Gram-negative organisms selected from Haemophilus influenzae, Moraxella catarrhalis, Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Chlamydia pneumoniae, Enterobacter cloacae, Enterobacter aerogenes, Klebsiella pneumoniae and Stenotrophomonas maltophilia.
For the C. pneumoniae isolates, stocks were thawed and diluted in CCM (Chlamydia Culture Media) to yield an inoculum containing ˜1×104 inclusion forming units/ml (IFUs/ml). A 100 μL aliquot of the inoculum was added to all wells of a microtitre plate containing HEp-2 (Human Epithelial (pharyngeal) cell line) cells grown to confluence. Microtitre plates were centrifuged for 1 hour at 1700 g., then incubated for 1 hour at 35° C. in 5% CO2. One hundred microliters of diluted test compounds, prepared as a 2-fold dilution series in CCM/cycloheximide was then added to the microtiter plates. After 72 hours incubation at 35° C. in 5°% CO2, the microtitre plates were stained with a murine monoclonal fluorescein-conjugated antibody (Kallestad Cat. #532 Roche Biomedical Products) in accordance with the manufacturer recommendations. Upon staining, the IFUs produced an apple-green color, visible against the red counter stained HEp-2 cells when viewed at 100× magnification. The MIC was defined as the lowest concentration of compound at which no IFUs were seen.
The minimum inhibitory concentration (MIC) was determined as the lowest concentration of compound that inhibited visible growth. A mirror reader was used to assist in determining the MIC endpoint.
Each of the listed Examples, as identified in the present application, was tested in at least one exemplified salt form. The listed Examples had a MIC≦2 μg/ml against a strain of at least one of the organisms listed above. For at least one strain of every organism listed above, at least one Example had a MIC≦32 μg/ml.
| Number | Date | Country | Kind |
|---|---|---|---|
| 0613208.8 | Jul 2006 | GB | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/EP2007/056664 | 7/3/2007 | WO | 00 | 12/18/2008 |
