Claims
- 1. An isolated nucleic acid encoding a protein which binds CD28 or CTLA4 comprising a contiguous nucleotide sequence derived from at least one T cell costimulatory molecule gene, the nucleotide sequence represented by a formula A-B-C-D-E, wherein
A comprises a nucleotide sequence of at least one first exon of a T cell costimulatory molecule gene, wherein the at least one first exon encodes a signal peptide domain, B comprises a nucleotide sequence of at least one second exon of a T cell costimulatory molecule gene, wherein the at least one second exon encodes an immunoglobulin variable region-like domain, C comprises a nucleotide sequence of at least one third exon of a T cell costimulatory molecule gene, wherein the at least one third exon encodes an immunoglobulin constant region-like domain, D comprises a nucleotide sequence of at least one fourth exon of a T cell costimulatory molecule gene, wherein the at least one fourth exon encodes a transmembrane domain, and E comprises a nucleotide sequence of at least one fifth exon of a T cell costimulatory molecule gene, wherein the at least one fifth exon encodes a cytoplasmic domain, with the proviso that E does not comprise a nucleotide sequence selected from a group consisting of SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29 and SEQ ID NO:31.
- 2. The isolated nucleic acid of claim 1 which is a cDNA.
- 3. The isolated nucleic acid of claim 2 which comprises a coding region of the cDNA.
- 4. The isolated nucleic acid of claim 1, wherein the nucleotide sequence is derived from a T cell costimulatory molecule gene encoding B7-1.
- 5. The isolated nucleic acid of claim 4, wherein B7-1 is murine.
- 6. The isolated nucleic acid of claim 4, wherein B7-1 is human.
- 7. The isolated nucleic acid of claim 5, wherein E comprises a nucleotide sequence shown in SEQ ID NO:4.
- 8. The isolated nucleic acid of claim 5, wherein E comprises a nucleotide sequence encoding an amino acid sequence shown in SEQ ID NO:5.
- 9. An isolated nucleic acid encoding a protein which binds CD28 or CTLA4 and is encoded by a T cell costimulatory molecule gene having
at least one first exon encoding a first cytoplasmic domain comprising a nucleotide sequence selected from the group consisting of a nucleotide sequence of SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29 and SEQ ID NO:31, and at least one second exon encoding a second cytoplasmic domain, wherein the isolated nucleic acid comprises a nucleotide sequence encoding the second cytoplasmic domain.
- 10. The isolated nucleic acid of claim 9 which comprises a coding region of a cDNA.
- 11. The isolated nucleic acid of claim 9 which does not comprise a nucleotide sequence encoding the first cytoplasmic domain.
- 12. The isolated nucleic acid of claim 9 wherein the T cell costimulatory molecule gene is B7-1.
- 13. The isolated nucleic acid of claim 12 wherein B7-1 is murine.
- 14. The isolated nucleic acid of claim 12 wherein B7-1 is human.
- 15. An isolated nucleic acid encoding a protein which binds CD28 or CTLA4 comprising a nucleotide sequence shown in SEQ ID NO: 1.
- 16. An isolated nucleic acid encoding a protein which binds CD28 or CTLA4 comprising a nucleotide sequence shown in SEQ ID NO:3.
- 17. An isolated nucleic acid encoding a cytoplasmic domain derived from a protein which binds CD28 or CTLA4, the nucleic acid comprising a nucleotide sequence shown in SEQ ID NO:4.
- 18. A recombinant expression vector comprising the nucleic acid molecule of claim 15.
- 19. A host cell which contains the recombinant expression vector of claim 18.
- 20. An isolated nucleic acid encoding a protein which binds CD28 or CTLA4 comprising a contiguous nucleotide sequence derived from at least one T cell costimulatory molecule gene, the nucleotide sequence represented by a formula A-B-C-D-E, wherein
A comprises a nucleotide sequence of at least one first exon of a T cell costimulatory molecule gene, wherein the at least one first exon encodes a signal peptide domain, B comprises a nucleotide sequence of at least one second exon of a T cell costimulatory molecule gene, wherein the at least one second exon encodes an immunoglobulin variable region-like domain, C comprises a nucleotide sequence of at least one third exon of a T cell costimulatory molecule gene, wherein the at least one third exon encodes an immunoglobulin constant region-like domain, D, which may or may not be present, comprises a nucleotide sequence of at least one fourth exon of a T cell costimulatory molecule gene, wherein the at least one fourth exon encodes a transmembrane domain, and E, which may or may not be present, comprises a nucleotide sequence of at least one fifth exon of a T cell costimulatory molecule gene, wherein the at least one fifth exon encodes a cytoplasmic domain, with the proviso that A does not comprise a nucleotide sequence selected from a group consisting of SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:39 and SEQ ID NO:41.
- 21. The isolated nucleic acid of claim 20 which is a cDNA.
- 22. The isolated nucleic acid of claim 21 which comprises a coding region of the cDNA.
- 23. The isolated nucleic acid of claim 20, wherein the nucleotide sequence is derived from a T cell costimulatory molecule gene encoding B7-2.
- 24. The isolated nucleic acid of claim 23, wherein B7-2 is murine.
- 25. The isolated nucleic acid of claim 23, wherein B7-2 is human.
- 26. The isolated nucleic acid of claim 24, wherein A comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 14.
- 27. An isolated nucleic acid encoding a protein which binds CD28 or CTLA4 and is encoded by a T cell costimulatory molecule gene having
at least one first exon encoding a first signal peptide domain comprising a nucleotide sequence selected from the group consisting of a nucleotide sequence of SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37 SEQ ID NO:39 and SEQ ID NO:41, and at least one second exon encoding a second signal peptide domain, wherein the isolated nucleic acid comprises a nucleotide sequence encoding the second signal peptide domain.
- 28. The isolated nucleic acid of claim 27 which comprises a coding region of a cDNA.
- 29. The isolated nucleic acid of claim 27 which does not comprise a nucleotide sequence encoding the first signal peptide domain.
- 30. The isolated nucleic acid of claim 27 wherein the T cell costimulatory molecule gene is B7-2.
- 31. The isolated nucleic acid of claim 30 wherein B7-2 is murine.
- 32. The isolated nucleic acid of claim 30 wherein B7-2 is human.
- 33. An isolated nucleic acid encoding a protein which binds CD28 or CTLA4 comprising a nucleotide sequence shown in SEQ ID NO:12.
- 34. An isolated nucleic acid encoding a signal peptide domain derived from a protein which binds CD28 or CTLA4, the nucleic acid comprising a nucleotide sequence shown in SEQ ID NO:14.
- 35. A recombinant expression vector comprising the nucleic acid molecule of claim 33.
- 36. A host cell which contains the recombinant expression vector of claim 35.
- 37. An isolated nucleic acid encoding a protein comprising a contiguous nucleotide sequence derived from at least one T cell costimulatory molecule gene, the nucleotide sequence represented by a formula A-B-C-D, wherein
A comprises a nucleotide sequence of at least one first exon of a T cell costimulatory molecule gene, wherein the at least one first exon encodes a signal peptide domain, B comprises a nucleotide sequence of at least one second exon of a T cell costimulatory molecule gene, wherein the at least one second exon encodes an immunoglobulin constant region-like domain, C comprises a nucleotide sequence of at least one third exon of a T cell costimulatory molecule gene, wherein the at least one third exon encodes a transmembrane domain, and D comprises a nucleotide sequence of at least one fourth exon of a T cell costimulatory molecule gene, wherein the at least one fourth exon encodes a cytoplasmic domain.
- 38. The isolated nucleic acid of claim 37 comprising a nucleotide sequence shown in SEQ ID NO:8.
- 39. The isolated nucleic acid of claim 37 comprising a nucleotide sequence shown in SEQ ID NO:10.
RELATED APPLICATIONS
[0001] This application is a divisional application of U.S. application Ser. No. 09/837,867, filed Apr. 17, 2001, entitled “B7 Domain-Specific Antibodies (As Amended)”; which is a divisional application of U.S. application Ser. No. 08/205,697 filed on Mar. 2, 1994, issued as U.S. Pat. No. 6,218,510 on Apr. 17, 2001, entitled “Novel Forms of T Cell Costimulatory Molecules and Uses Therefor”. The contents of all of the aforementioned applications are hereby incorporated by reference.
GOVERNMENT FUNDING
[0002] Work described herein was supported under CA-40216 and GM46883 awarded by the National Instiutes of Health. The U.S. government therefore may have certain rights in this invention.
Divisions (2)
|
Number |
Date |
Country |
Parent |
09837867 |
Apr 2001 |
US |
Child |
10643768 |
Aug 2003 |
US |
Parent |
08205697 |
Mar 1994 |
US |
Child |
09837867 |
Apr 2001 |
US |