Bacillus licheniformis chromosome

Information

  • Patent Application
  • 20110086407
  • Publication Number
    20110086407
  • Date Filed
    December 17, 2010
    13 years ago
  • Date Published
    April 14, 2011
    13 years ago
Abstract
The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis. The present invention also relates to isolated genes of the chromosome of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the genes as well as methods for producing biologically active substances encoded by the genes and to methods of using the isolated genes of the complete chromosome of Bacillus licheniformis.
Description
REFERENCE TO A SEQUENCE LISTING

This application contains a Sequence Listing in computer readable form, which is incorporated herein by reference.


BACKGROUND OF THE INVENTION

1. Field of the Invention


The present invention relates to an isolated polynucleotide molecule comprising the complete chromosome of Bacillus licheniformis. The present invention also relates to features (genes) of the complete chromosomal DNA molecule of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the features as well as methods for producing biologically active substances encoded by the features and to methods of using the isolated features derived from the complete chromosomal DNA molecule of Bacillus licheniformis.


2. Description of the Related Art


Microbes, which make up most of the earth's biomass, have evolved for some 3.8 billion years. They are found in virtually every environment, surviving and thriving in extremes of heat, cold, radiation, pressure, salt, acidity, and darkness. Often in these environments, no other forms of life are found and the only nutrients come from inorganic matter. The diversity and range of their environmental adaptations indicate that microbes long ago “solved” many problems for which scientists are still actively seeking solutions. The value in determining the complete genome sequence of microbes is that it provides a detailed blueprint for the organism revealing all of the biochemical pathways, substrates, intermediates, and end products as well as regulatory networks, and evolutionary relationships to other microbes. A complete manifest of proteins, both structural and catalytic, is encoded as a list of features in the DNA molecule comprising the genome, as well as their likely cellular location.


Knowledge about the enormous range of microbial capacities has broad and far-reaching implications for environmental, energy, health, and industrial applications, such as cleanup of toxic-waste, production of novel therapeutic and preventive agents (drugs and vaccines), energy generation and development of renewable energy sources, production of chemical catalysts, reagents, and enzymes to improve efficiency of industrial processes, management of environmental carbon, nitrogen and nutrient cycling, detection of disease-causing organisms and monitoring of the safety of food and water supplies, use of genetically altered bacteria as living sensors (biosensors) to detect harmful chemicals in soil, air, or water, and understanding of specialized systems used by microbial cells to live in natural environments.



Bacillus licheniformis is a gram positive spore-forming bacterium that is widely distributed as a saprophytic organism in the environment. Unlike most other bacilli that are predominantly aerobic, Bacillus licheniformis is a facultative anaerobe which may allow it to grow in additional ecological niches. This species produces a diverse assortment of extracellular enzymes that are believed to contribute to the process of nutrient cycling in nature (Claus, D. and Berkeley, R. C. W., 1986, In Bergey's Manual of Systematic Bacteriology, Vol. 2., eds. Sneath, P. H. A. et al., Williams and Wilkins Co., Baltimore, Md., pp. 1105-1139). Certain Bacillus licheniformis isolates are capable of denitrification, however, the relevance of this characteristic to environmental denitrification may be small since the species generally persists in soil as endospores (Alexander, M., 1977, Introduction to Soil Microbiology. John Wiley and Sons, Inc., New York).


There are numerous industrial and agricultural uses for Bacillus licheniformis and its extracellular products. The species has been used for decades in the manufacture of industrial enzymes including several proteases, α-amylase, penicillinase, pentosanase, cycloglucosyltransferase, β-mannanase, and several pectinolytic enzymes, owing largely to its ability to secrete sizeable amounts of degradative enzymes. Bacillus licheniformis is also used to produce peptide antibiotics such as bacitracin and proticin, in addition to a number of specialty chemicals such as citric acid, inosine, inosinic acid, and poly-γ-glutamic acid. The proteases from Bacillus licheniformis are used in the detergent industry as well as for dehairing and batting of leather (Eveleigh, D. E., 1981, Scientific American 245, 155-178). Amylases from Bacillus licheniformis are deployed for the hydrolysis of starch, desizing of textiles, and sizing of paper (Erickson, R. J., 1976, In Microbiology, ed. Schlesinger, D. (Am. Soc. Microbiol., Washington, D.C.), pp. 406-419.). Certain strains of Bacillus licheniformis have shown efficacy to destroy fungal pathogens affecting maize, grasses, and vegetable crops (U.S. Pat. No. 5,589,381; U.S. Pat. No. 5,665,354). As an endospore-forming bacterium, the ability of the organism to survive under unfavorable environmental conditions may enhance its potential as a natural control agent.



Bacillus licheniformis can be differentiated from other bacilli on the basis of metabolic and physiological tests (Logan, N. A. and Berkeley, R. C. W., 1981, In The Aerobic Endospore-Forming Bacteria Classification and Identification, eds. Berkeley, R. C. W. and Goodfellow, M., Academic Press, Inc., London, pp. 106-140; O'Donnell, A. G., Norris, J. R., Berkeley, R. C. W., Claus, D., Kanero, T., Logan, N. A., and Nozaki, R., 1980, Internat. J. Systematic Bacteriol. 30: 448-459). However, biochemical and phenotypic characteristics may be ambiguous among closely related species. Lapidus et al. (Lapidus, A., Galleron, N., Andersen, J. T., Jørgensen, P. L. Ehrlich, S. D., and Sorokin, A., 2002, FEMS Microbiol. Lett. 209: 23-30) recently constructed a physical map of the Bacillus licheniformis chromosome using a PCR approach, and established a number of regions of co-linearity where gene content and organization were ostensibly conserved with the Bacillus subtilis chromosome.


It would be advantageous to the art to have available the complete primary structure of the chromosomal DNA molecule of the Bacillus licheniformis type strain ATCC 14580. With the complete chromosome data in hand, it should be possible to do comparative genomics and proteomics studies that can lead to improved industrial strains as well as to a better understanding of genome evolution among closely-related bacilli in the subtilis-licheniformis group.


It is an object of the present invention to provide an isolated polynucleotide with the sequence of the complete chromosome of Bacillus licheniformis.


SUMMARY OF THE INVENTION

The present invention relates to an isolated polynucleotide of the complete chromosomal DNA molecule of Bacillus licheniformis ATCC 14580 having the nucleotide sequence of SEQ ID NO: 1.


The present invention also relates to isolated features (genes) of the complete chromosomal DNA molecule of Bacillus licheniformis ATCC 14580 encoding biologically active substances, selected from the group consisting of:


(a) a gene comprising a nucleotide sequence having at least 60% identity with any of the polynucleotides of SEQ ID NOs: 2-4198; and


(b) a gene comprising a nucleotide sequence which hybridizes under at least medium stringency conditions with any of the polynucleotides of SEQ ID NOs: 2-4198, or a complementary strand thereof.


The present invention also relates to biologically active substances encoded by the isolated genes, and nucleic acid constructs, vectors, and host cells comprising the genes.


The present invention also relates to methods for producing such substances having biological activity comprising (a) cultivating a recombinant host cell comprising a nucleic acid construct comprising a gene encoding the biologically active substance under conditions suitable for production of the biologically active substance; and (b) recovering the biologically active substance.


The present invention also relates to methods for monitoring differential expression of a plurality of genes in a first bacterial cell relative to expression of the same genes in one or more second bacterial cells, comprising:


(a) adding a mixture of detection reporter-labeled nucleic acids isolated from the bacterial cells to a substrate containing an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198, under conditions where the detection reporter-labeled nucleic acids hybridize to complementary sequences of the Bacillus licheniformis genes on the array, wherein the nucleic acids from the first bacterial cell and the one or more second bacterial cells are labeled with a first detection reporter and one or more different second detection reporters, respectively; and


(b) examining the array under conditions wherein the relative expression of the genes in the bacterial cells is determined by the observed detection signal of each spot on the array in which (i) the Bacillus licheniformis genes on the array that hybridize to the nucleic acids obtained from either the first or the one or more second bacterial cells produce a distinct first detection signal or one or more second detection signals, respectively, and (ii) the Bacillus licheniformis genes on the array that hybridize to the nucleic acids obtained from both the first and one or more second bacterial produce a distinct combined detection signal.


The present invention also relates to methods for isolating a gene encoding an enzyme, comprising:


(a) adding a mixture of labeled first nucleic acid probes, isolated from a microbial strain cultured on medium without an inducing substrate, and labeled second nucleic acid probes, isolated from the microbial strain cultured on medium with the inducing substrate, to an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198, under conditions where the labeled nucleic acid probes hybridize to complementary sequences of the Bacillus licheniformis genes on the array, wherein the first nucleic acid probes are labeled with a first reporter and the second nucleic acid probes are labeled with a second reporter;


(b) examining the array under conditions wherein the relative expression of the genes of the microbial strain is determined by the observed hybridization reporter signal of each spot on the array in which (i) the Bacillus licheniformis genes on the array that hybridize to the first nucleic acid probes produce a distinct first hybridization reporter signal or the second nucleic acid probes produce a distinct second hybridization reporter signal, and (ii) the Bacillus licheniformis genes on the array that hybridize to both the first and second nucleic acid probes produce a distinct combined hybridization reporter signal; and


(c) isolating a gene from the microbial strain that encodes an enzyme that degrades or converts the substrate.


The present invention also relates to genes isolated by such methods and nucleic acid constructs, vectors, and host cells containing the genes.


DEFINITIONS

Biologically active substance: The term “biologically active substance” is defined herein as any substance which is encoded by a single gene or a series of genes (contiguous or non-contiguous) composing a biosynthetic or metabolic pathway or operon or may be the direct or indirect result of the product of a single gene or products of a series of genes of the Bacillus licheniformis chromosome. Such substances include, but are not limited to, biopolymers, metabolites, and cellular structures and components (e.g., ribosome, flagella, etc.). For purposes of the present invention, biological activity is determined according to procedures known in the art such as those described by Carpenter and Sabatini, 2004, Nature 5: 11-22; Sordie et al., 2003, Proceedings of the National Academy of Sciences USA 100: 11964-11969; Braun and LaBaer, 2003, TRENDS in Biotechnology 21: 383-388; and Kaberdin and McDowall, 2003, Genome Research 13: 1961-1965.


In the methods of the present invention, the biopolymer may be any biopolymer. The term “biopolymer” is defined herein as a chain (or polymer) of identical, similar, or dissimilar subunits (monomers). The biopolymer may be, but is not limited to, a nucleic acid, polyamine, polyol, polypeptide (or polyamide), or polysaccharide.


In a preferred aspect, the biopolymer is a polypeptide. The polypeptide may be any polypeptide having a biological activity of interest. The term “polypeptide” is not meant herein to refer to a specific length of the encoded product and, therefore, encompasses peptides, oligopeptides, and proteins. The term “polypeptide” also encompasses naturally occurring allelic variations.


In a preferred aspect, the polypeptide is an antibody, antigen, antimicrobial peptide, enzyme, growth factor, hormone, immunodilator, neurotransmitter, receptor, reporter protein, structural protein, transcription factor, and transporter.


In a more preferred aspect, the polypeptide is an oxidoreductase, transferase, hydrolase, lyase, isomerase, or ligase. In a most preferred aspect, the polypeptide is an alpha-glucosidase, aminopeptidase, amylase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, esterase, alpha-galactosidase, beta-galactosidase, glucoamylase, glucocerebrosidase, alpha-glucosidase, beta-glucosidase, invertase, laccase, lipase, mannosidase, mutanase, oxidase, pectinolytic enzyme, peroxidase, phospholipase, phytase, polyphenoloxidase, proteolytic enzyme, ribonuclease, transglutaminase, urokinase, xylanase.


In another preferred aspect, the polypeptide is a collagen or gelatin.


In another preferred aspect, the biopolymer is a polysaccharide. The polysaccharide may be any polysaccharide, including, but not limited to, a mucopolysaccharide (e.g., heparin and hyaluronic acid) and a nitrogen-containing polysaccharide (e.g., chitin). In a more preferred aspect, the polysaccharide is hyaluronic acid (hyaluronan).


In the methods of the present invention, the metabolite may be any metabolite. The metabolite may be encoded by one or more genes, such as a biosynthetic or metabolic pathway. The term “metabolite” encompasses both primary and secondary metabolites. Primary metabolites are products of primary or general metabolism of a cell, which are concerned with energy metabolism, growth, and structure. Secondary metabolites are products of secondary metabolism (see, for example, R. B. Herbert, The Biosynthesis of Secondary Metabolites, Chapman and Hall, New York, 1981).


The primary metabolite may be, but is not limited to, an amino acid, fatty acid, nucleoside, nucleotide, sugar, triglyceride, vitamin.


The secondary metabolite may be, but is not limited to, an alkaloid, coumarin, flavonoid, polyketide, quinine, steroid, peptide, or terpene. In a preferred aspect, the secondary metabolite is an antibiotic, antifeedant, attractant, bacteriocide, fungicide, hormone, insecticide, or rodenticide.


Isolated biologically active substance: The term “isolated biologically active substance” is defined herein as a substance which is at least about 20% pure, preferably at least about 40% pure, more preferably about 60% pure, even more preferably about 80% pure, most preferably about 90% pure, and even most preferably about 95% pure, as determined by SDS-PAGE, HPLC, capillary electrophoresis, or any other method used in the art.


Substantially pure biologically active substance or pure biologically active substance: The term “substantially pure biologically active substance” is defined herein as a biologically active substance preparation which contains at most 10%, preferably at most 8%, more preferably at most 6%, more preferably at most 5% by weight, more preferably at most 4%, at most 3%, even more preferably at most 2%, most preferably at most 1%, and even most preferably at most 0.5% by weight of other material with which it is natively associated. It is, therefore, preferred that the substantially pure biologically active substance is at least 92% pure, preferably at least 94% pure, more preferably at least 95% pure, more preferably at least 96% pure, more preferably at least 96% pure, more preferably at least 97% pure, even more preferably at least 98% pure, most preferably at least 99%, and even most preferably at least 99.5% pure by weight of the total material present in the preparation. The term “pure biologically active substance” is defined as a biologically active substance preparation which contains no other material with which it is natively associated.


The biologically active substances of the present invention are preferably in a substantially pure form. In particular, it is preferred that the biologically active substances are in “essentially pure form”, i.e., that the biologically active substance preparation is essentially free of other material with which it is natively associated. This can be accomplished, for example, by preparing the biologically active substance by means of well-known recombinant methods or by classical purification methods.


Identity: The relatedness between two amino acid sequences or between two nucleotide sequences is described by the parameter “identity”.


For purposes of the present invention, the degree of identity between two amino acid sequences is determined by the Smith-Waterman Protein method for the Genematcher2, as implemented by Paracel Inc. (Pasadena, Calif.), or the BLASTP method as described by Altschul et al., 1990, Journal of Molecular Biology 215: 403-410.


For purposes of the present invention, the degree of identity between two nucleotide sequences is determined by the Smith Waterman nucleotide method for the Genematcher2 or BLASTN for the BlastMachine as implemented by Paracel Inc.


Polypeptide Fragment: The term “polypeptide fragment” is defined herein as a polypeptide, which retains biological activity, having one or more amino acids deleted from the amino and/or carboxyl terminus of a polypeptide encoded by any of the genes of the present invention, i.e., polypeptides of SEQ ID NOs: 4199-8395. Preferably, a fragment contains at least 80%, preferably at least 85%, more preferably at least 90%, even more preferably at least 95%, and most preferably at least 97% of the amino acid residues of the mature encoded polypeptide product.


Subsequence: The term “subsequence” is defined herein as a polynucleotide comprising a nucleotide sequence of any of SEQ ID NOs: 2-4198 except that one or more nucleotides have been deleted from the 5′ and/or 3′ end. Preferably, a subsequence contains at least 80%, preferably at least 85%, more preferably at least 90%, even more preferably at least 95%, and most preferably at least 97% of the nucleotides of any of the genes of the present invention.


Allelic variant: The term “allelic variant” denotes herein any of two or more alternative forms of a gene occupying the same chromosomal locus. Allelic variation arises naturally through mutation, and may result in polymorphism within populations. Gene mutations can be silent (no change in the encoded polypeptide) or may encode polypeptides having altered amino acid sequences. An allelic variant of a polypeptide is a polypeptide encoded by an allelic variant of a gene.


Substantially pure polynucleotide or pure polynucleotide: The term “substantially pure polynucleotide” as used herein refers to a polynucleotide preparation free of other extraneous or unwanted nucleotides and is in a form suitable for use within genetically engineered production systems. Thus, a substantially pure polynucleotide contains at most 10%, preferably at most 8%, more preferably at most 6%, more preferably at most 5%, more preferably at most 4%, more preferably at most 3%, even more preferably at most 2%, most preferably at most 1%, and even most preferably at most 0.5% by weight of other polynucleotide material with which it is natively associated. A substantially pure polynucleotide may, however, include naturally occurring 5′ and 3′ untranslated regions, such as promoters and terminators. It is preferred that the substantially pure polynucleotide is at least 92% pure, preferably at least 94% pure, more preferably at least 95% pure, moire preferably at least 96% pure, more preferably at least 96% pure, more preferably at least 97% pure, more preferably at least 98% pure, most preferably at least 99%, and even most preferably at least 99.5% pure by weight. The polynucleotides of the present invention are preferably in a substantially pure form. In particular, it is preferred that the polynucleotides disclosed herein are in “essentially pure form”, i.e., that the polynucleotide preparation is essentially free of other polynucleotide material with which it is natively associated. The term “pure polynucleotide” is defined as a polynucleotide preparation which contains no other material with which it is natively associated.


Nucleic acid construct: The term “nucleic acid construct” as used herein refers to a nucleic acid molecule, either single- or double-stranded, which is isolated from a naturally occurring gene or which has been modified to contain segments of nucleic acids in a manner that would not otherwise exist in nature. The term nucleic acid construct is synonymous with the term “expression cassette” when the nucleic acid construct contains the control sequences required for expression of a coding sequence of the present invention.


Control sequence: The term “control sequences” is defined herein to include all components, which are necessary or advantageous for the expression of a biologically active substance of the present invention. Each control sequence may be native or foreign to the polynucleotide encoding the substance. Such control sequences include, but are not limited to, a leader, propeptide sequence, promoter, signal peptide sequence, and transcription terminator. At a minimum, the control sequences include a promoter, and transcriptional and translational stop signals. The control sequences may be provided with linkers for the purpose of introducing specific restriction sites facilitating ligation of the control sequences with the coding region of the polynucleotide encoding a biologically active substance.


Operably linked: The term “operably linked” as used herein refers to a configuration in which a control sequence is placed at an appropriate position relative to the coding sequence of the DNA sequence, such that the control sequence directs the expression of a biologically active substance.


Coding sequence: When used herein the term “coding sequence” is intended to cover a nucleotide sequence, which directly specifies the amino acid sequence of its protein product. The boundaries of the coding sequence are generally determined by an open reading frame, which usually begins with the ATG start codon or alternative start codons such as GTG and TTG.


Expression: The term “expression” includes any step involved in the production of a biologically active substance including, but not limited to, transcription, post-transcriptional modification, translation, post-translational modification, and secretion.


Expression vector: The term “expression vector” herein covers a DNA molecule, linear or circular, that comprises a segment encoding a biologically active substance of the invention, and which is operably linked to additional segments that provide for its transcription.


Host cell: The term “host cell”, as used herein, includes any cell type which is susceptible to transformation, transfection, conjugation, electroporation, etc. with a nucleic acid construct, plasmid, or vector.







DETAILED DESCRIPTION OF THE INVENTION


Bacillus licheniformis Chromosome and Features (Genes) Thereof


The present invention relates to an isolated polynucleotide of the complete chromosomal DNA molecule of Bacillus licheniformis ATCC 14580 having the nucleotide sequence of SEQ ID NO: 1. Bacillus licheniformis ATCC 14580, consists of a circular molecule of 4,222,336 base pairs with a mean G+C content of 46.2%. The chromosome contains 4208 predicted protein-coding genes (SEQ ID NOs: 2-4198) with an average size of 873 bp, 7 rRNA operons, and 72 tRNA genes. The deduced amino acid sequences of the 4208 predicted protein-coding genes are shown in SEQ ID NOs: 4199-8395. SEQ ID NO: 4210 corresponds to SEQ ID NO: 2, SEQ ID NO: 4211 corresponds to SEQ ID NO: 3, SEQ ID NO: 4212 corresponds to SEQ ID NO: 4, etc. The predicted functions of the 4208 gene products are shown in Table 1.


The Bacillus licheniformis chromosome possesses regions that are markedly co-linear with the chromosomes of Bacillus subtilis and Bacillus halodurans, and approximately 80% of the predicted genes have Bacillus subtilis orthologues.


The present invention also relates to isolated features (genes) of the complete chromosomal DNA molecule of Bacillus licheniformis ATCC 14580 encoding biologically active substances, selected from the group consisting of:


(a) a gene comprising a nucleotide sequence having at least 60% identity with any of the nucleotide sequences of SEQ ID NOs: 2-4198; and


(b) a gene comprising a nucleotide sequence which hybridizes under at least medium stringency conditions with any of the genes of SEQ ID NOs: 2-4198, or a complementary strand thereof.


In a first aspect, the present invention relates to isolated genes, which have a degree of identity to the nucleotide sequences of any of SEQ ID NOs: 2-4198 of at least about 60%, preferably at least about 65%, more preferably at least about 70%, more preferably at least about 75%, more preferably at least about 80%, more preferably at least about 85%, even more preferably at least about 90%, most preferably at least about 95%, and even most preferably at least about 97%, which encode biologically active substances having a particular biological activity (hereinafter “homologous biologically active substances”).


In a second aspect, the present invention relates to isolated genes comprising nucleotide sequences which hybridize under very low stringency conditions, preferably low stringency conditions, more preferably medium stringency conditions, more preferably medium-high stringency conditions, even more preferably high stringency conditions, and most preferably very high stringency conditions with any of (i) the genes of SEQ ID NOs: 2-4198, or subsequences thereof, or (ii) complementary strands thereof (J. Sambrook, E. F. Fritsch, and T. Maniatus, 1989, Molecular Cloning, A Laboratory Manual, 2d edition, Cold Spring Harbor, New York). Subsequences of SEQ ID NOs: 2-4198 may be at least 100 nucleotides or preferably at least 200 nucleotides. Moreover, the subsequences may encode fragments of a gene product which have biological activity. The biologically active substances may also be biologically active allelic variants of the biologically active substances.


The nucleotide sequences of SEQ ID NOs: 2-4198 or subsequences thereof, as well as the amino acid sequences of SEQ ID NOs: 4199-8395 or fragments thereof, may be used to design nucleic acid probes to identify and clone DNA encoding biologically active substances from strains of different genera or species according to methods well known in the art. In particular, such probes can be used for hybridization with the genomic DNA of the genus or species of interest, following standard Southern blotting procedures, in order to identify and isolate the corresponding gene therein. Such probes can be considerably shorter than the entire sequence, but should be at least 14, preferably at least 25, more preferably at least 35 nucleotides in length, such as at least 70 nucleotides in length. It is preferred, however, that the nucleic acid probes are at least 100 nucleotides in length. For example, the nucleic acid probes may be at least 200 nucleotides, at least 300 nucleotides, at least 400 nucleotides, or at least 500 nucleotides in length. Even longer probes may be used, e.g., nucleic acid probes which are at least 600 nucleotides, at least 700 nucleotides, at least 800 nucleotides, or at least 900 nucleotides in length. Both DNA and RNA probes can be used. The probes are typically labeled for detecting the corresponding gene (for example, with 32P, 3H, 35S, biotin, or avidin). Such probes are encompassed by the present invention.


A genomic DNA library prepared from such other organisms may, therefore, be screened for DNA which hybridizes with the probes described above and which encodes a biologically active substance. Genomic DNA from such other organisms may be separated by agarose or polyacrylamide gel electrophoresis, or other separation techniques. DNA from the libraries or the separated DNA may be transferred to and immobilized on nitrocellulose or other suitable carrier material. In order to identify a clone or DNA which is homologous with any of SEQ ID NOs: 2-4198 or subsequences thereof, the carrier material is used in a Southern blot.


For purposes of the present invention, hybridization indicates that a polynucleotide hybridizes to a labeled gene having the nucleotide sequence shown in any of SEQ ID NOs: 2-4198, complementary strands thereof, or subsequences thereof, under very low to very high stringency conditions. Molecules to which the nucleic acid probe hybridizes under these conditions can be detected using X-ray film.


In a preferred aspect, the nucleic acid probe is any of the genes of SEQ ID NOs: 2-4198, or subsequences thereof. In another preferred aspect, the nucleic acid probe is the mature coding region of any of the genes of SEQ ID NOs: 2-4198. In another preferred aspect, the nucleic acid probe is the gene of any of SEQ ID NOs: 2-4198 contained in Bacillus licheniformis ATCC 14580. In another preferred aspect, the nucleic acid probe is the mature coding region of any of the genes of SEQ ID NOs: 2-4198 contained in Bacillus licheniformis ATCC 14580.


For long probes of at least 100 nucleotides in length, very low to very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and either 25% formamide for very low and low stringencies, 35% formamide for medium and medium-high stringencies, or 50% formamide for high and very high stringencies, following standard Southern blotting procedures.


For long probes of at least 100 nucleotides in length, the carrier material is finally washed three times each for 15 minutes using 2×SSC, 0.2% SDS preferably at least at 45° C. (very low stringency), more preferably at least at 50° C. (low stringency), more preferably at least at 55° C. (medium stringency), more preferably at least at 60° C. (medium-high stringency), even more preferably at least at 65° C. (high stringency), and most preferably at least at 70° C. (very high stringency).


For short probes which are about 14 nucleotides to about 70 nucleotides in length, stringency conditions are defined as prehybridization, hybridization, and washing post-hybridization at about 5° C. to about 10° C. below the calculated Tm using the calculation according to Bolton and McCarthy (1962, Proceedings of the National Academy of Sciences USA 48:1390) in 0.9 M NaCl, 0.09 M Tris-HCl pH 7.6, 6 mM EDTA, 0.5% NP-40, 1×Denhardt's solution, 1 mM sodium pyrophosphate, 1 mM sodium monobasic phosphate, 0.1 mM ATP, and 0.2 mg of yeast RNA per ml following standard Southern blotting procedures.


For short probes which are about 14 nucleotides to about 70 nucleotides in length, the carrier material is washed once in 6×SCC plus 0.1% SDS for 15 minutes and twice each for 15 minutes using 6×SSC at 5′C to 10° C. below the calculated Tm.


Under salt-containing hybridization conditions, the effective Tm is what controls the degree of identity required between the probe and the filter bound DNA for successful hybridization. The effective Tm may be determined using the formula below to determine the degree of identity required for two DNAs to hybridize under various stringency conditions.





Effective Tm=81.5+16.6(log M[Na+])+0.41(% G+C)−0.72(% formamide)


The % G+C content of any of the genes of SEQ ID NOs: 2-4198 can easily be determined. For medium stringency, for example, the concentration of formamide is 35% and the Na+ concentration for 5×SSPE is 0.75 M. Applying this formula to these values, the Effective Tm in ° C. can be calculated. Another relevant relationship is that a 1% mismatch of two DNAs lowers the Tm 1.4° C. To determine the degree of identity required for two DNAs to hybridize under medium stringency conditions at 42° C., the following formula is used:





% Homology=100−[(Effective Tm−Hybridization Temperature)/1.41]


Applying this formula, the degree of identity required for two DNAs to hybridize under medium stringency conditions at 42° C. can be calculated.


Similar calculations can be made under other stringency conditions, as defined herein.


The present invention also relates to isolated polynucleotides obtained by (a) hybridizing a population of DNA under very low, low, medium, medium-high, high, or very high stringency conditions with any of (i) the genes of SEQ ID NOs: 2-4198, or subsequences thereof, or (ii) complementary strands thereof; and (b) isolating the hybridizing polynucleotide from the population of DNA. In a preferred aspect, the hybridizing polynucleotide encodes a polypeptide of any of SEQ ID NOs: 2-4198, or homologous polypeptides thereof.


In a third aspect, the present invention relates to isolated polypeptides having amino acid sequences which have a degree of identity to any of SEQ ID NOs: 4199-8395 of at least about 60%, preferably at least about 65%, more preferably at least about 70%, more preferably at least about 75%, more preferably at least about 80%, more preferably at least about 85%, even more preferably at least about 90%, most preferably at least about 95%, and even most preferably at least about 97%, which have biological activity (hereinafter “homologous polypeptides”). In a preferred aspect, the homologous polypeptides have an amino acid sequence which differs by ten amino acids, preferably by five amino acids, more preferably by four amino acids, even more preferably by three amino acids, most preferably by two amino acids, and even most preferably by one amino acid from the amino acid sequences of SEQ ID NOs: 4199-8395.


The polypeptides of the present invention preferably comprise the amino acid sequence of any of SEQ ID NOs: 4199-8395 or an allelic variant thereof; or a fragment thereof that has biological activity. In a more preferred aspect, the polypeptides of the present invention comprise the amino acid sequence of any of SEQ ID NOs: 4199-8395. In another preferred aspect, the polypeptides of the present invention comprise the mature polypeptide region of any of SEQ ID NOs: 4199-8395, or an allelic variant thereof; or a fragment thereof that has biological activity. In another preferred aspect, the polypeptides of the present invention comprise the mature polypeptide region of any of SEQ ID NOs: 4199-8395. In another preferred aspect, the polypeptides of the present invention consist of the amino acid sequence of any of SEQ ID NOs: 4199-8395 or an allelic variant thereof; or a fragment thereof that has biological activity. In another preferred aspect, the polypeptides of the present invention consist of the amino acid sequence of any of SEQ ID NOs: 4199-8395. In another preferred aspect, the polypeptides consist of the mature polypeptide region of any of SEQ ID NOs: 4199-8395 or an allelic variant thereof; or a fragment thereof that has biological activity. In another preferred aspect, the polypeptides consist of the mature polypeptide region of any of SEC) ID NOs: 4199-8395.


In a fourth aspect, the present invention relates to isolated substances having biological activity which are encoded by polynucleotides which hybridize, as described above, under very low stringency conditions, preferably low stringency conditions, more preferably medium stringency conditions, more preferably medium-high stringency conditions, even more preferably high stringency conditions, and most preferably very high stringency conditions with which hybridize under very low stringency conditions, preferably low stringency conditions, more preferably medium stringency conditions, more preferably medium-high stringency conditions, even more preferably high stringency conditions, and most preferably very high stringency conditions with any of (i) the genes of SEQ ID NOs: 2-4198, or subsequences thereof, or (ii) complementary strands thereof. A subsequence of any of SEQ ID NOs: 2-4198 may be at least 100 nucleotides or preferably at least 200 nucleotides. Moreover, the subsequence may encode a fragment, e.g., a polypeptide fragment, which has biological activity.


Nucleic Acid Constructs

The present invention also relates to nucleic acid constructs comprising an isolated gene or isolated genes (e.g., operon) of the present invention operably linked to one or more control sequences which direct the expression of the coding sequence in a suitable host cell under conditions compatible with the control sequences.


An isolated gene(s) of the present invention may be manipulated in a variety of ways to provide for production of a biologically active substance encoded directly or indirectly by the gene(s). Manipulation of the nucleotide sequence prior to its insertion into a vector may be desirable or necessary depending on the expression vector. The techniques for modifying nucleotide sequences utilizing recombinant DNA methods are well known in the art.


The control sequence may be an appropriate promoter sequence, a nucleotide sequence which is recognized by a host cell for expression of the gene(s) encoding the biologically active substance. The promoter sequence contains transcriptional control sequences which mediate the expression of the biologically active substance. The promoter may be any nucleotide sequence which shows transcriptional activity in the host cell of choice including mutant, truncated, and hybrid promoters, and may be obtained from genes encoding extracellular or intracellular polypeptides or biologically active substances either homologous or heterologous to the host cell.


Examples of suitable promoters for directing the transcription of the nucleic acid constructs of the present invention, especially in a bacterial host cell, are the promoters obtained from the E. coli lac operon, Streptomyces coelicolor agarase gene (dagA), Bacillus subtilis levansucrase gene (sacB), Bacillus licheniformis alpha-amylase gene (amyL), Bacillus stearothermophilus maltogenic amylase gene (amyM), Bacillus amyloliquefaciens alpha-amylase gene (amyQ), Bacillus licheniformis penicillinase gene (penP), Bacillus subtilis xylA and xylB genes, and prokaryotic beta-lactamase gene (Villa-Kamaroff et al., 1978, Proceedings of the National Academy of Sciences USA 75: 3727-3731), as well as the tac promoter (DeBoer et al., 1983, Proceedings of the National Academy of Sciences USA 80: 21-25). Further promoters are described in “Useful proteins from recombinant bacteria” in Scientific American, 1980, 242: 74-94; and in Sambrook et al., 1989, supra.


The control sequence may also be a suitable transcription terminator sequence, a sequence recognized by a host cell to terminate transcription. The terminator sequence is operably linked to the 3′ terminus of the gene encoding the biologically active substance. Any terminator which is functional in the host cell of choice may be used in the present invention.


The control sequence may also be a signal peptide coding region that codes for an amino acid sequence linked to the amino terminus of a polypeptide and directs the encoded polypeptide into the cell's secretory pathway. The 5′ end of the coding sequence of the nucleotide sequence may inherently contain a signal peptide coding region naturally linked in translation reading frame with the segment of the coding region which encodes the secreted polypeptide. Alternatively, the 5′ end of the coding sequence may contain a signal peptide coding region which is foreign to the coding sequence. The foreign signal peptide coding region may be required where the coding sequence does not naturally contain a signal peptide coding region. Alternatively, the foreign signal peptide coding region may simply replace the natural signal peptide coding region in order to enhance secretion of the polypeptide. However, any signal peptide coding region which directs the expressed polypeptide into the secretory pathway of a host cell of choice may be used in the present invention.


Effective signal peptide coding regions for bacterial host cells are the signal peptide coding regions obtained from the genes for Bacillus NCIB 11837 maltogenic amylase, Bacillus stearothermophilus alpha-amylase, Bacillus licheniformis subtilisin, Bacillus licheniformis beta-lactamase, Bacillus stearothermophilus neutral proteases (nprT, nprS, nprM), and Bacillus subtilis prsA. Further signal peptides are described by Simonen and Palva, 1993, Microbiological Reviews 57: 109-137.


The control sequence may also be a propeptide coding region that codes for an amino acid sequence positioned at the amino terminus of a polypeptide. The resultant polypeptide is known as a proenzyme or propolypeptide (or a zymogen in some cases). A propolypeptide is generally inactive and can be converted to a mature active polypeptide by catalytic or autocatalytic cleavage of the propeptide from the propolypeptide. The propeptide coding region may be obtained from the genes for Bacillus subtilis alkaline protease (aprE) and Bacillus subtilis neutral protease (nprT).


Where both signal peptide and propeptide regions are present at the amino terminus of a polypeptide, the propeptide region is positioned next to the amino terminus of a polypeptide and the signal peptide region is positioned next to the amino terminus of the propeptide region.


It may also be desirable to add regulatory sequences which allow the regulation of the expression of a biologically active substance relative to the growth of the host cell. Examples of regulatory systems are those which cause the expression of the gene to be turned on or off in response to a chemical or physical stimulus, including the presence of a regulatory compound. Regulatory systems in prokaryotic systems include the lac, tac, and trp operator systems. Other examples of regulatory sequences are those which allow for gene amplification. In eukaryotic systems, these include the dihydrofolate reductase gene which is amplified in the presence of methotrexate, and the metallothionein genes which are amplified with heavy metals. In these cases, the nucleotide sequence encoding the biologically active substance would be operably linked with the regulatory sequence.


Expression Vectors

The present invention also relates to recombinant expression vectors comprising an isolated gene of the present invention, a promoter, and transcriptional and translational stop signals. The various nucleic acid and control sequences described above may be joined together to produce a recombinant expression vector which may include one or more convenient restriction sites to allow for insertion or substitution of the nucleotide sequence encoding the polypeptide at such sites. Alternatively, a gene of the present invention may be expressed by inserting the nucleotide sequence or a nucleic acid construct comprising the sequence into an appropriate vector for expression. In creating the expression vector, the coding sequence is located in the vector so that the coding sequence is operably linked with the appropriate control sequences for expression.


The recombinant expression vector may be any vector (e.g., a plasmid or virus) which can be conveniently subjected to recombinant DNA procedures and can bring about the expression of a gene of the present invention. The choice of the vector will typically depend on the compatibility of the vector with the host cell into which the vector is to be introduced. The vectors may be linear or closed circular plasmids.


The vector may be an autonomously replicating vector, i.e., a vector which exists as an extrachromosomal entity, the replication of which is independent of chromosomal replication, e.g., a plasmid, an extrachromosomal element, a minichromosome, or an artificial chromosome. The vector may contain any means for assuring self-replication. Alternatively, the vector may be one which, when introduced into the host cell, is integrated into the genome and replicated together with the chromosome(s) into which it has been integrated. Furthermore, a single vector or plasmid or two or more vectors or plasmids which together contain the total DNA to be introduced into the genome of the host cell, or a transposon may be used.


The vectors of the present invention preferably contain one or more selectable markers which permit easy selection of transformed cells. A selectable marker is a gene the product of which provides for biocide or viral resistance, resistance to heavy metals, prototrophy to auxotrophs, and the like.


Examples of bacterial selectable markers are the dal genes from Bacillus subtilis or Bacillus licheniformis, or markers which confer antibiotic resistance such as ampicillin, kanamycin, chloramphenicol or tetracycline resistance.


The vectors of the present invention preferably contain an element(s) that permits integration of the vector into the host cell's genome or autonomous replication of the vector in the cell independent of the genome.


For integration into the host cell genome, the vector may rely on portions of the sequence of the gene or any other element of the vector for integration of the vector into the genome by homologous or nonhomologous recombination. Alternatively, the vector may contain additional nucleotide sequences for directing integration by homologous recombination into the genome of the host cell. The additional nucleotide sequences enable the vector to be integrated into the host cell genome at a precise location(s) in the chromosome(s). To increase the likelihood of integration at a precise location, the integrational elements should preferably contain a sufficient number of nucleotides, such as 100 to 10,000 base pairs, preferably 400 to 10,000 base pairs, and most preferably 800 to 10,000 base pairs, which are highly homologous with the corresponding target sequence to enhance the probability of homologous recombination. The integrational elements may be any sequence that is homologous with the target sequence in the genome of the host cell. Furthermore, the integrational elements may be non-encoding or encoding nucleotide sequences. On the other hand, the vector may be integrated into the genome of the host cell by non-homologous recombination.


For autonomous replication, the vector may further comprise an origin of replication enabling the vector to replicate autonomously in the host cell in question. The origin of replication may be any plasmid replicator mediating autonomous replication which functions in a cell. The term “origin of replication” or “plasmid replicator” is defined herein as a sequence that enables a plasmid or vector to replicate in vivo. Examples of bacterial origins of replication are the origins of replication of plasmids pBR322, pUC19, pACYC177, and pACYC184 permitting replication in E. coli, and pUB110, pE194, pTA1060, and pAMβ1 permitting replication in Bacillus.


More than one copy of a gene of the present invention may be inserted into the host cell to increase production of the gene product. An increase in the copy number of the gene can be obtained by integrating at least one additional copy of the sequence into the host cell genome or by including an amplifiable selectable marker gene with a gene of the present invention where cells containing amplified copies of the selectable marker gene, and thereby additional copies of the gene of the present invention, can be selected for by cultivating the cells in the presence of the appropriate selectable agent.


The procedures used to ligate the elements described above to construct the recombinant expression vectors of the present invention are well known to one skilled in the art (see, e.g., Sambrook et al., 1989, supra).


Host Cells

The present invention also relates to recombinant host cells, comprising an isolated gene of the present invention, where the host cells are advantageously used in the recombinant production of a biologically active substance encoded by the gene. A vector comprising a gene of the present invention is introduced into a host cell so that the vector is maintained as a chromosomal integrant or as a self-replicating extra-chromosomal vector as described earlier. The term “host cell” encompasses any progeny of a parent cell that is not identical to the parent cell due to mutations that occur during replication. The choice of a host cell will to a large extent depend upon the gene encoding the biologically active substance and its source.


The host cell may be any unicellular microorganism, e.g., a prokaryote.


Useful unicellular cells are bacterial cells such as gram positive bacteria including, but not limited to, a Bacillus cell, e.g., Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus cereus, Bacillus circulans, Bacillus clausii, Bacillus coagulans, Bacillus fastidiosus, Bacillus firmus, Bacillus lautus, Bacillus lentus, Bacillus licheniformis, Bacillus macerans, Bacillus megaterium, Bacillus methanolicus, Bacillus purnilus, Bacillus sphaericus, Bacillus stearothermophilus, Bacillus subtilis, and Bacillus thuringiensis; or a Streptomyces cell, e.g., Streptomyces lividans and Streptomyces murinus, or gram negative bacteria such as E. coli and Pseudomonas sp. In a preferred aspect, the bacterial host cell is a Bacillus lentus, Bacillus licheniformis, Bacillus stearothermophilus, or Bacillus subtilis cell. In another preferred aspect, the Bacillus cell is an alkalophilic Bacillus.


The introduction of a vector into a bacterial host cell may, for instance, be effected by protoplast transformation (see, e.g., Chang and Cohen, 1979, Molecular General Genetics 168: 111-115), using competent cells (see, e.g., Young and Spizizen, 1961, Journal of Bacteriology 81: 823-829, or Dubnau and Davidoff-Abelson, 1971, Journal of Molecular Biology 56: 209-221), electroporation (see, e.g., Shigekawa and Dower, 1988, Biotechniques 6: 742-751), or conjugation (see, e.g., Koehler and Thorne, 1987, Journal of Bacteriology 169: 5771-5278).


Methods of Production

The present invention also relates to methods for producing a biologically active substance of the present invention comprising (a) cultivating a strain, which in its wild-type form is capable of producing the biologically active substance, under conditions conducive for production of the biologically active substance; and (b) recovering the biologically active substance. Preferably, the strain is of the genus Bacillus, and more preferably Bacillus licheniformis.


The present invention also relates to methods for producing a biologically active substance of the present invention comprising (a) cultivating a host cell under conditions conducive for production of the biologically active substance; and (b) recovering the biologically active substance.


The present invention also relates to methods for producing a biologically active substance of the present invention comprising (a) cultivating a host cell under conditions conducive for production of the biologically active substance, wherein the host cell comprises a mutant polynucleotide comprising at least one mutation in the coding region of any of SEQ ID NOs: 2-4198, wherein the mutant polynucleotide encodes a biologically active substance which consists of SEQ ID NOs: 4199-8395, respectively, and (b) recovering the biologically active substance.


In the production methods of the present invention, the cells are cultivated in a nutrient medium suitable for production of the biologically active substance using methods known in the art. For example, the cell may be cultivated by shake flask cultivation, and small-scale or large-scale fermentation (including continuous, batch, fed-batch, or solid state fermentations) in laboratory or industrial fermentors performed in a suitable medium and under conditions allowing the biologically active substance to be expressed and/or isolated. The cultivation takes place in a suitable nutrient medium comprising carbon and nitrogen sources and inorganic salts, using procedures known in the art. Suitable media are available from commercial suppliers or may be prepared according to published compositions (e.g., in catalogues of the American Type Culture Collection). If the biologically active substance is secreted into the nutrient medium, the biologically active substance can be recovered directly from the medium. If the biologically active substance is not secreted, it can be recovered from cell lysates.


The biologically active substances may be detected using methods known in the art that are specific for the polypeptides. These detection methods may include use of specific antibodies, formation of an enzyme product, or disappearance of an enzyme substrate. For example, an enzyme assay may be used to determine the activity of an enzyme.


The resulting biologically active substances may be recovered by methods known in the art. For example, the biologically active substances may be recovered from the nutrient medium by conventional procedures including, but not limited to, centrifugation, filtration, extraction, spray-drying, evaporation, or precipitation.


The biologically active substances of the present invention may be purified by a variety of procedures known in the art including, but not limited to, chromatography (e.g., ion exchange, affinity, hydrophobic, chromatofocusing, and size exclusion), electrophoretic procedures (e.g., preparative isoelectric focusing), differential solubility (e.g., ammonium sulfate precipitation), SDS-PAGE, or extraction (see, e.g., Protein Purification, J.-C. Janson and Lars Ryden, editors, VCH Publishers, New York, 1989).


Plants

The present invention also relates to a transgenic plant, plant part, or plant cell which has been transformed with a gene encoding a biologically active substance of the present invention so as to express and produce the biologically active substance in recoverable quantities. The biologically active substance may be recovered from the plant or plant part. Alternatively, the plant or plant part containing the recombinant biologically active substance may be used as such for improving the quality of a food or feed, e.g., improving nutritional value, palatability, and rheological properties, or to destroy an antinutritive factor.


The transgenic plant can be dicotyledonous (a dicot) or monocotyledonous (a monocot). Examples of monocot plants are grasses, such as meadow grass (blue grass, Poa), forage grass such as festuca, lolium, temperate grass, such as Agrostis, and cereals, e.g., wheat, oats, rye, barley, rice, sorghum, and maize (corn).


Examples of dicot plants are tobacco, legumes, such as lupins, potato, sugar beet, pea, bean and soybean, and cruciferous plants (family Brassicaceae), such as cauliflower, rape seed, and the closely related model organism Arabidopsis thaliana.


Examples of plant parts are stem, callus, leaves, root, fruits, seeds, and tubers as well as the individual tissues comprising these parts, e.g., epidermis, mesophyll, parenchyma, vascular tissues, meristems. In the present context, also specific plant cell compartments, such as chloroplast, apoplast, mitochondria, vacuole, peroxisomes and cytoplasm are considered to be a plant part. Furthermore, any plant cell, whatever the tissue origin, is considered to be a plant part. Likewise, plant parts such as specific tissues and cells isolated to facilitate the utilisation of the invention are also considered plant parts e.g. embryos, endosperms, aleurone and seeds coats.


Also included within the scope of the present invention are the progeny of such plants, plant parts, and plant cells.


The transgenic plant or plant cell expressing a biologically active substance of the present invention may be constructed in accordance with methods known in the art. Briefly, the plant or plant cell is constructed by incorporating one or more expression constructs encoding a biologically active substance of the present invention into the plant host genome and propagating the resulting modified plant or plant cell into a transgenic plant or plant cell.


The expression construct is conveniently a nucleic acid construct which comprises a gene encoding a biologically active substance of the present invention operably linked with appropriate regulatory sequences required for expression of the nucleotide sequence in the plant or plant part of choice. Furthermore, the expression construct may comprise a selectable marker useful for identifying host cells into which the expression construct has been integrated and DNA sequences necessary for introduction of the construct into the plant in question (the latter depends on the DNA introduction method to be used).


The choice of regulatory sequences, such as promoter and terminator sequences and optionally signal or transit sequences is determined, for example, on the basis of when, where, and how the biologically active substance is desired to be expressed. For instance, the expression of the gene encoding a biologically active substance of the present invention may be constitutive or inducible, or may be developmental, stage or tissue specific, and the gene product may be targeted to a specific tissue or plant part such as seeds or leaves. Regulatory sequences are, for example, described by Tague et al., 1988, Plant Physiology 86: 506.


For constitutive expression the 35S-CaMV, the maize ubiquitin 1 and the rice actin 1 promoter may be used (Franck et al., 1980. Cell 21: 285-294, Christensen A H, Sharrock R A and Quail, 1992, Plant Mo. Biol. 18: 675-689; Zhang W, McElroy D. and Wu R., 1991, Plant Cell 3: 1155-1165). Organ-specific promoters may be, for example, a promoter from storage sink tissues such as seeds, potato tubers, and fruits (Edwards and Coruzzi, 1990, Ann. Rev. Genet. 24: 275-303), or from metabolic sink tissues such as meristems (Ito et al., 1994, Plant Mol. Biol. 24: 863-878), a seed specific promoter such as the glutelin, prolamin, globulin, or albumin promoter from rice (Wu et al., 1998, Plant and Cell Physiology 39: 885-889), a Vicia faba promoter from the legumin B4 and the unknown seed protein gene from Vicia faba (Conrad et al., 1998, Journal of Plant Physiology 152: 708-711), a promoter from a seed oil body protein (Chen et al., 1998, Plant and Cell Physiology 39: 935-941), the storage protein napA promoter from Brassica napus, or any other seed specific promoter known in the art, e.g., as described in WO 91/14772. Furthermore, the promoter may be a leaf specific promoter such as the rbcs promoter from rice or tomato (Kyozuka et al., 1993, Plant Physiology 102: 991-1000, the chlorella virus adenine methyltransferase gene promoter (Mitra and Higgins, 1994, Plant Molecular Biology 26: 85-93), or the aldP gene promoter from rice (Kagaya et al., 1995, Molecular and General Genetics 248: 668-674), or a wound inducible promoter such as the potato pint promoter (Xu et al., 1993, Plant Molecular Biology 22: 573-588). Likewise, the promoter may inducible by abiotic treatments such as temperature, drought or alterations in salinity or induced by exogenously applied substances that activate the promoter, e.g., ethanol, oestrogens, plant hormones like ethylene, abscisic acid and gibberellic acid and heavy metals.


A promoter enhancer element may also be used to achieve higher expression of the enzyme in the plant. For instance, the promoter enhancer element may be an intron which is placed between the promoter and the nucleotide sequence encoding a biologically active substance of the present invention. For instance, Xu et al., 1993, supra disclose the use of the first intron of the rice actin 1 gene to enhance expression.


The selectable marker gene and any other parts of the expression construct may be chosen from those available in the art.


The nucleic acid construct is incorporated into the plant genome according to conventional techniques known in the art, including Agrobacterium-mediated transformation, virus-mediated transformation, microinjection, particle bombardment, biolistic transformation, and electroporation (Gasser et al., 1990, Science 244: 1293; Potrykus, 1990, Bio/Technology 8; 535: Shimamoto et al., 1989, Nature 338: 274).


Presently, Agrobacterium tumefaciens-mediated gene transfer is the method of choice for generating transgenic dicots (for a review, see Hooykas and Schilperoort, 1992, Plant Molecular Biology 19: 15-38). However it can also be used for transforming monocots, although other transformation methods are generally preferred for these plants. Presently, the method of choice for generating transgenic monocots is particle bombardment (microscopic gold or tungsten particles coated with the transforming DNA) of embryonic calli or developing embryos (Christou, 1992, Plant Journal 2: 275-281; Shimamoto, 1994, Current Opinion Biotechnology 5: 158-162; Vasil et al., 1992, Bio/Technology 10: 667-674). An alternative method for transformation of monocots is based on protoplast transformation as described by Omirulleh et al., 1993, Plant Molecular Biology 21: 415-428.


Following transformation, the transformants having incorporated therein the expression construct are selected and regenerated into whole plants according to methods well-known in the art. Often the transformation procedure is designed for the selective elimination of selection genes either during regeneration or in the following generations by using, for example, co-transformation with two separate T-DNA constructs or site specific excision of the selection gene by a specific recombinase.


The present invention also relates to methods for producing a biologically active substance of the present invention comprising (a) cultivating a transgenic plant or a plant cell comprising a gene encoding a biologically active substance of the present invention under conditions conducive for production of the biologically active substance; and (b) recovering the biologically active substance.


Removal or Reduction of Biologically Active Substance

The present invention also relates to methods for producing a mutant of a parent cell, which comprises disrupting or deleting all or a portion of a gene encoding a biologically active substance of the present invention, which results in the mutant cell producing less of the biologically active substance than the parent cell when cultivated under the same conditions.


The mutant cell may be constructed by reducing or eliminating expression of a gene encoding or regulatory synthesis of a biologically active substance of the present invention using methods well known in the art, for example, insertions, disruptions, replacements, or deletions. The gene to be modified or inactivated may be, for example, the coding region or a part thereof essential for activity, or a regulatory element of the gene required for the expression of the coding region. An example of such a regulatory or control sequence may be a promoter sequence or a functional part thereof, i.e., a part that is sufficient for affecting expression of the gene. Other control sequences for possible modification include, but are not limited to, a leader, propeptide sequence, signal peptide sequence, transcription terminator, and transcriptional activator.


Modification or inactivation of the gene may be performed by subjecting the parent cell to mutagenesis and selecting for mutant cells in which expression of the gene has been reduced or eliminated. The mutagenesis, which may be specific or random, may be performed, for example, by use of a suitable physical or chemical mutagenizing agent, by use of a suitable oligonucleotide, or by subjecting the DNA sequence to PCR generated mutagenesis. Furthermore, the mutagenesis may be performed by use of any combination of these mutagenizing agents.


Examples of a physical or chemical mutagenizing agent suitable for the present purpose include ultraviolet (UV) irradiation, hydroxylamine, N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), O-methyl hydroxylamine, nitrous acid, ethyl methane sulphonate (EMS), sodium bisulphite, formic acid, and nucleotide analogues.


When such agents are used, the mutagenesis is typically performed by incubating the parent cell to be mutagenized in the presence of the mutagenizing agent of choice under suitable conditions, and screening and/or selecting for mutant cells exhibiting reduced or no expression of the gene.


Modification or inactivation of the nucleotide sequence may be accomplished by introduction, substitution, or removal of one or more nucleotides in the gene or a regulatory element required for the transcription or translation thereof. For example, nucleotides may be inserted or removed so as to result in the introduction of a stop codon, the removal of the start codon, or a change in the open reading frame. Such modification or inactivation may be accomplished by site-directed mutagenesis or PCR generated mutagenesis in accordance with methods known in the art. Although, in principle, the modification may be performed in vivo, i.e., directly on the cell expressing the nucleotide sequence to be modified, it is preferred that the modification be performed in vitro as exemplified below.


An example of a convenient way to eliminate or reduce expression of a nucleotide sequence by a cell of choice is based on techniques of gene replacement, gene deletion, or gene disruption. For example, in the gene disruption method, a nucleic acid sequence corresponding to the endogenous nucleotide sequence is mutagenized in vitro to produce a defective nucleic acid sequence which is then transformed into the parent cell to produce a defective gene. By homologous recombination, the defective nucleic acid sequence replaces the endogenous nucleotide sequence. It may be desirable that the defective nucleotide sequence also encodes a marker that may be used for selection of transformants in which the nucleotide sequence has been modified or destroyed. In a particularly preferred aspect, the nucleotide sequence is disrupted with a selectable marker such as those described herein.


Alternatively, modification or inactivation of the nucleotide sequence may be performed by established anti-sense techniques using a sequence complementary to the nucleotide sequence. More specifically, expression of the nucleotide sequence by a cell may be reduced or eliminated by introducing a sequence complementary to the nucleic acid sequence of the gene that may be transcribed in the cell and is capable of hybridizing to the mRNA produced in the cell. Under conditions allowing the complementary anti-sense nucleotide sequence to hybridize to the mRNA, the amount of protein translated is thus reduced or eliminated.


The present invention further relates to a mutant cell of a parent cell which comprises a disruption or deletion of a nucleotide sequence encoding the biologically active substance or a control sequence thereof, which results in the mutant cell producing less of the biologically active substance than the parent cell.


The biologically active substance-deficient mutant cells so created are particularly useful as host cells for the expression of homologous and/or heterologous substances, such as polypeptides. Therefore, the present invention further relates to methods for producing a homologous or heterologous substance comprising (a) cultivating the mutant cell under conditions conducive for production of the substance; and (b) recovering the substance. The term “heterologous substances” is defined herein as substances which are not native to the host cell, a native substance in which modifications have been made to alter the native sequence, or a native substance whose expression is quantitatively altered as a result of a manipulation of the host cell by recombinant DNA techniques.


In a further aspect, the present invention relates to a method for producing a protein product essentially free of a biologically active substance by fermentation of a cell which produces both a biologically active substance of the present invention as well as the protein product of interest by adding an effective amount of an agent capable of inhibiting activity of the biologically active substance to the fermentation broth before, during, or after the fermentation has been completed, recovering the product of interest from the fermentation broth, and optionally subjecting the recovered product to further purification.


In accordance with this aspect of the invention, it is possible to remove at least 60%, preferably at least 75%, more preferably at least 85%, still more preferably at least 95%, and most preferably at least 99% of the biologically active substance. Complete removal of biologically active substance may be obtained by use of this method.


The methods used for cultivation and purification of the product of interest may be performed by methods known in the art.


The methods of the present invention for producing an essentially biologically active substance-free product is of particular interest in the production of prokaryotic polypeptides, in particular bacterial proteins such as enzymes. The enzyme may be selected from, e.g., an amylolytic enzyme, lipolytic enzyme, proteolytic enzyme, cellulytic enzyme, oxidoreductase, or plant cell-wall degrading enzyme. Examples of such enzymes include an aminopeptidase, amylase, amyloglucosidase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, esterase, galactosidase, beta-galactosidase, glucoamylase, glucose oxidase, glucosidase, haloperoxidase, hemicellulase, invertase, isomerase, laccase, ligase, lipase, lyase, mannosidase, oxidase, pectinolytic enzyme, peroxidase, phytase, phenoloxidase, polyphenoloxidase, proteolytic enzyme, ribonuclease, transferase, transglutaminase, or xylanase. The biologically active substance-deficient cells may also be used to express heterologous proteins of pharmaceutical interest such as hormones, growth factors, receptors, and the like.


It will be understood that the term “prokaryotic polypeptides” includes not only native polypeptides, but also those polypeptides, e.g., enzymes, which have been modified by amino acid substitutions, deletions or additions, or other such modifications to enhance activity, thermostability, pH tolerance and the like.


In a further aspect, the present invention relates to a product of a protein or substance essentially free of a biologically active substance of the invention, produced by a method of the present invention.


Compositions

The present invention also relates to compositions comprising a biologically active substance of the present invention. Preferably, the compositions are enriched in the biologically active substance. The term “enriched” indicates that the biologically active substance of the composition has been increased, e.g., with an enrichment factor of 1.1.


The composition may comprise a biologically active substance of the invention as the major component, e.g., a mono-component composition. Alternatively, the composition may comprise multiple biologically active substances, for example, multiple enzymes, such as an aminopeptidase, amylase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, esterase, alpha-galactosidase, beta-galactosidase, glucoamylase, alpha-glucosidase, beta-glucosidase, haloperoxidase, invertase, laccase, lipase, mannosidase, oxidase, pectinolytic enzyme, peptidoglutaminase, peroxidase, phytase, polyphenoloxidase, proteolytic enzyme, ribonuclease, transglutaminase, or xylanase.


The compositions may be prepared in accordance with methods known in the art and may be in the form of a liquid or a dry composition. For instance, the composition may be in the form of a granulate or a microgranulate. The biologically active substance to be included in the composition may be stabilized in accordance with methods known in the art.


Methods for Using the Bacillus licheniformis Chromosome


The present invention also relates to methods for using the Bacillus licheniformis chromosome.


The chromosome of Bacillus licheniformis serves as a reservoir of new genes/proteins that have likely environmental, energy, health, and industrial applications (e.g., enzymes, antibiotics, biochemicals). A clear extension of this is that the newly discovered molecules can be used as starting points for further improvements via well-established gene shuffling, directed evolution, and protein engineering methods. Additionally, regions or motifs (e.g., signal peptides, active sites, substrate-binding regions) from the newly discovered molecules may be employed to derive novel chimeras with industrially advantageous properties.


The genes encoded in the chromosome may be used for monitoring global gene expression during the life cycle of the organism or during industrial fermentations (e.g., implemented on DNA microarrays). By monitoring global gene expression, for example, improved processes for industrial fermentation can be implemented with greater efficiency and economy.


The chromosome is useful in comparative evolutionary and ecological studies. For example, dozens of Bacillus licheniformis isolates can be readily compared on a global scale by hybridization of their genomic DNAs to a microarray fabricated from the reference strain presented in this case (so-called comparative genomic hybridization). Using this method, one can compare various isolates to look for similarities/differences among geographical and environmental niches or among biocontrol strains versus saprophytic isolates.


The chromosome sequence may be used to construct the metabolic blueprint for Bacillus licheniformis that includes all catabolic and anabolic pathways, signaling pathways, regulatory networks, growth substrates, biochemical intermediates, end products, electron donors/acceptors and others. In doing so, it is possible to modify the metabolic machinery of the organism by deleting unwanted pathways and/or adding enzymes/pathways from other organisms to generate useful chemicals and intermediates.


The pathways and components that contribute to production of extracellular and surface proteins in Bacillus licheniformis can be extracted from the chromosomal sequence. This affords opportunities for improved production of extracellular proteins by genetic manipulation of the secretion machinery.


The chromosome data allows deduction of the essential genes for Bacillus licheniformis (either by comparison to related bacteria such as Bacillus subtilis or by systematic gene-by-gene knock outs). Thus it has become possible to design custom-made strains which contain only the genes that are essential for production of specific proteins or metabolites (so-called cell factory concept).


The chromosome data may be used to construct interspecies hybrids between Bacillus licheniformis and other bacteria. Venter et al., 2003, Proc. Nat. Acad. Sci. USA 100, 15440-15445 have shown that it is possible to construct an entire virus genome from smaller DNA segments. Thus, segments of the Bacillus licheniformis chromosome may be employed to derive novel chromosomal segments or even entire chimeric chromosomes for specific applications.


In a preferred aspect, methods for using the Bacillus licheniformis chromosome include host improvement, e.g., secretion of a protein or metabolite, genome shuffling, construction of new genomes, metabolic engineering and pathway reconstruction, carrier for heterologous expression vectors, microarrays as described herein, identification of polypeptides in proteomics analyses, and comparative genomics with other Bacillus species or related organisms.


Methods for Isolating Genes

The present invention also relates to methods for isolating a gene encoding a biologically active substance from a microbial strain. The method comprises first the addition of a mixture of first labeled nucleic acid probes, isolated from a microbial strain cultured on medium without an inducing substrate, and a mixture of second labeled nucleic acid probes, isolated from the microbial strain cultured on medium with the inducing substrate, to an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198, under conditions where the labeled nucleic acid probes hybridize to complementary sequences of the Bacillus licheniformis genes on the array. The first nucleic acid probes are labeled with a first reporter and the second nucleic acid probes are labeled with a second reporter. The array is then examined under conditions wherein the relative expression of the genes of the microbial strain is determined by the observed hybridization reporter signal of each spot on the array in which (i) the Bacillus licheniformis genes on the array that hybridize to the first nucleic acid probes produce a distinct first hybridization reporter signal or to the second nucleic acid probes produce a distinct second hybridization reporter signal, and (ii) the Bacillus licheniformis genes on the array that hybridize to both the first and second nucleic acid probes produce a distinct combined hybridization reporter signal. The probe is then sequenced to isolate from the microbial strain the corresponding gene that encodes an enzyme that degrades or converts the substrate.


Enzymes. The gene of interest may encode any enzyme including an oxidoreductase, transferase, hydrolase, lyase, isomerase, or ligase. In a preferred aspect, the enzyme is an acylase, alpha-glucosidase, amidase, aminopeptidase, amylase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, dextrinase, endoglucanase, esterase, galactanase, alpha-galactosidase, beta-galactosidase, glucoamylase, glucanase, glucocerebrosidase, alpha-glucosidase, beta-glucosidase, hemicellulase, invertase, laccase, lignase, lipase, lysin, mannosidase, mutanase, oxidase, pectinolytic enzyme, peroxidase, phosphatase, phospholipase, phytase, polyphenoloxidase, proteolytic enzyme, pullulanase, ribonuclease, transglutaminase, urokinase, or xylanase.


Inducing Substrate. The inducing substrate may be any substrate that is subject to the action of an enzyme, i.e., that degrades or converts the substrate. In a preferred aspect, the inducing substrate is lignin or a lignin-containing material. In a more preferred aspect, the lignin-containing material is lignocellulose. In another preferred aspect, the inducing substrate is cellulose. In another preferred aspect, the inducing substrate is hemicellulose. In another preferred aspect, the inducing substrate is pectin. In another preferred aspect, the inducing substrate is a lipid. In another preferred aspect, the inducing substrate is phospholipid. In another preferred aspect, the inducing substrate is phytic acid. In another preferred aspect, the inducing substrate is protein. In another preferred aspect, the inducing substrate is a starch. In another preferred aspect, the inducing substrate is a medium that is low in nutrients such as amino acids, carbon, nitrogen, phosphate, or iron.


In a more preferred aspect, the protein substrate is blood, casein, egg, gelatin, gluten, milk protein, or soy protein. In another more preferred aspect, the lignin-containing material is hardwood thermomechanical pulp. In another more preferred aspect, the lignocellulose is corn stover. In another more preferred aspect, the lignocellulose is white poplar. In another more preferred aspect, the lignocellulose is rice straw. In another more preferred aspect, the lignocellulose is switch grass.


Microbial Strains. In the methods of the present invention, the microbial strain may be any microbial strain. The strain is cultured on a suitable nutrient medium with and without a substrate of interest. The strain cultured on medium without the substrate is used as a reference for identifying differences in expression of the same or similar complement of genes in the strain cultured on medium with substrate. The strain may be a wild-type, mutant, or recombinant strain.


In the methods of the present invention, the microbial strain is preferably a bacterium. In a more preferred aspect, the bacterium is a Bacillus, Pseudomonas, or Streptomyces strain or E. coli.


The Bacillus strain may be any Bacillus strain. In a preferred aspect, the Bacillus strain is Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus cereus, Bacillus circulans, Bacillus clausii, Bacillus coagulans, Bacillus fastidiosus, Bacillus firmus, Bacillus lautus, Bacillus lentos, Bacillus licheniformis, Bacillus macerans, Bacillus megaterium, Bacillus methanolicus, Bacillus pumilus, Bacillus sphaericus, Bacillus stearothermophilus, Bacillus subtilis, or Bacillus thuringiensis. It will be understood that the term “Bacillus” also encompasses relatives of Bacillus such as Paenibacillus, Oceanobacillus, and the like.


The Pseudomonas strain may be any Pseudomonas strain. In a preferred aspect, the Pseudomonas strain is Pseudomonas acidovorans, Pseudomonas aeruginosa, Pseudomonas alcaligenes, Pseudomonas anguilliseptica, Pseudomonas abtimicrobica, Pseudomonas aurantiaca, Pseudomonas aureofaciens, Pseudomonas beijerinckii, Pseudomonas boreopolis, Pseudomonas chlororaphis, Pseudomonas citronellolis, Pseudomonas cocovenenans, Pseudomonas diminuta, Pseudomonas doudoroffii, Pseudomonas echinoides, Pseudomonas elongata, Pseudomonas fluorescens, Pseudomonas fragi, Pseudomonas halophobica, Pseudomonas huttiensis, Pseudomonas indigofera, Pseudomonas lanceolata, Pseudomonas lemoignei, Pseudomonas lundensis, Pseudomonas mendocina, Pseudomonas mephitica, Pseudomonas mucidolens, Pseudomonas oleovorans, Pseudomonas phenazinium, Pseudomonas pictorium, Pseudomonas putida, Pseudomonas resinovorans, Pseudomonas saccharophila, Pseudomonas stanieri, Pseudomonas stutzeri, Pseudomonas taetrolens, or Pseudomonas vesicularis.


The Streptomyces strain may be any Streptomyces strain. In a preferred aspect, the Streptomyces strain is Streptomyces lividans. In another preferred aspect, the Streptomyces strain is Streptomyces murinus.


Microarrays. The term “an array of Bacillus licheniformis genes” is defined herein as a linear or two-dimensional array of preferably discrete elements of an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198 (e.g., synthetic oligonucleotides of, for example, 40-60 nucleotides), wherein each discrete element has a finite area, formed on the surface of a solid support. It shall be understood that the term “Bacillus licheniformis genes” encompasses nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198.


The term “microarray” is defined herein as an array of Bacillus licheniformis gene elements having a density of discrete of Bacillus licheniformis gene elements of at least about 100/cm2, and preferably at least about 1000/cm2. The Bacillus licheniformis gene elements in a microarray have typical dimensions, e.g., diameters, in the range of between about 10 to about 250 μm, preferably in the range of between about 10 to about 200 μm, more preferably in the range of between about 20 to about 150 μm, even more preferably in the range of between about 20 to about 100 μm, most preferably in the range of between about 50 to about 100 μm, and even most preferably in the range of between about 80 to about 100 μm, and are separated from other gene elements in the microarray by about the same distance.


Methods and instruments for forming microarrays on the surface of a solid support are well known in the art. See, for example, U.S. Pat. No. 5,807,522; U.S. Pat. No. 5,700,637; and U.S. Pat. No. 5,770,151. The instrument may be an automated device such as described in U.S. Pat. No. 5,807,522.


The term “a substrate containing an array of Bacillus licheniformis genes” is defined herein as a solid support having deposited on the surface of the support one or more of a plurality of Bacillus licheniformis genes, as described herein, for use in detecting binding of labeled nucleic acids to the Bacillus licheniformis genes.


The substrate may, in one aspect, be a glass support (e.g., glass slide) having a hydrophilic or hydrophobic coating on the surface of the support, and an array of distinct random nucleic acid fragments bound to the coating, where each distinct random nucleic acid fragment is disposed at a separate, defined position.


Each microarray in the substrate preferably contains at least 103 distinct Bacillus licheniformis in a surface area of less than about 5 or 6 cm2. Each distinct Bacillus licheniformis gene (i) is disposed at a separate, defined position on the array, (ii) has a length of at least 50 bp, and (iii) is present in a defined amount between about 0.1 femtomoles and 100 nanomoles or higher if necessary.


For a hydrophilic coating, the glass slide is coated by placing a film of a polycationic polymer with a uniform thickness on the surface of the slide and drying the film to form a dried coating. The amount of polycationic polymer added should be sufficient to form at least a monolayer of polymers on the glass surface. The polymer film is bound to the surface via electrostatic binding between negative silyl-OH groups on the surface and charged cationic groups in the polymers. Such polycationic polymers include, but are not limited to, polylysine and polyarginine.


Another coating strategy employs reactive aldehydes to couple DNA to the slides (Schena et al., 1996, Proceedings of the National Academy of Science USA 93: 10614-10619; Heller at al., 1997, Proceedings of the National Academy of Science USA 94: 2150-2155).


Alternatively, the surface may have a relatively hydrophobic character, i.e., one that causes aqueous medium deposited on the surface to bead. A variety of known hydrophobic polymers, such as polystyrene, polypropylene, or polyethylene, have desirable hydrophobic properties, as do glass and a variety of lubricant or other hydrophobic films that may be applied to the support surface. A support surface is “hydrophobic” if an aqueous droplet applied to the surface does not spread out substantially beyond the area size of the applied droplet, wherein the surface acts to prevent spreading of the droplet applied to the surface by hydrophobic interaction with the droplet.


In another aspect, the substrate may be a multi-cell substrate where each cell contains a microarray of Bacillus licheniformis and preferably an identical microarray, formed on a porous surface. For example, a 96-cell array may typically have array dimensions between about 12 and 244 mm in width and 8 and 400 mm in length, with the cells in the array having width and length dimension of 1/12 and ⅛ the array width and length dimensions, respectively, i.e., between about 1 and 20 in width and 1 and 50 mm in length.


The solid support may include a water-impermeable backing such as a glass slide or rigid polymer sheet, or other non-porous material. Formed on the surface of the backing is a water-permeable film, which is formed of porous material. Such porous materials include, but are not limited to, nitrocellulose membrane nylon, polypropylene, and polyvinylidene difluoride (PVDF) polymer. The thickness of the film is preferably between about 10 and 1000 μm. The film may be applied to the backing by spraying or coating, or by applying a preformed membrane to the backing.


Alternatively, the solid support may be simply a filter composed of nitrocellulose, nylon, polypropylene, or polyvinylidene difluoride (PVDF) polymer, or, for that matter, any material suitable for use.


The film surface may be partitioned into a desirable array of cells by water-impermeable grid lines typically at a distance of about 100 to 2000 μm above the film surface. The grid lines can be formed on the surface of the film by laying down an uncured flowable resin or elastomer solution in an array grid, allowing the material to infiltrate the porous film down to the backing, and then curing the grid lines to form the cell-array substrate.


The barrier material of the grid lines may be a flowable silicone, wax-based material, thermoset material (e.g., epoxy), or any other useful material. The grid lines may be applied to the solid support using a narrow syringe, printing techniques, heat-seal stamping, or any other useful method known in the art.


Each well preferably contains a microarray of distinct Bacillus licheniformis genes. “Distinct Bacillus licheniformis genes” as applied to the genes forming a microarray is defined herein as an array member which is distinct from other array members on the basis of a different Bacillus licheniformis gene sequence or oligo sequence thereof, and/or different concentrations of the same or distinct Bacillus licheniformis genes and/or different mixtures of distinct Bacillus licheniformis genes or different-concentrations of Bacillus licheniformis genes. Thus an array of “distinct Bacillus licheniformis genes” may be an array containing, as its members, (i) distinct Bacillus licheniformis genes which may have a defined amount in each member, (ii) different, graded concentrations of a specific Bacillus licheniformis gene, and/or (iii) different-composition mixtures of two or more distinct Bacillus licheniformis genes.


It will be understood, however, that in the methods of the present invention, any type of substrate known in the art may be used.


The delivery of a known amount of a selected Bacillus licheniformis gene to a specific position on the support surface is preferably performed with a dispensing device equipped with one or more tips for insuring reproducible deposition and location of the Bacillus licheniformis genes and for preparing multiple arrays. Any dispensing device known in the art may be used in the methods of the present invention. See, for example, U.S. Pat. No. 5,807,522.


For liquid-dispensing on a hydrophilic surface, the liquid will have less of a tendency to bead, and the dispensed volume will be more sensitive to the total dwell time of the dispenser tip in the immediate vicinity of the support surface.


For liquid-dispensing on a hydrophobic surface, flow of fluid from the tip onto the support surface will continue from the dispenser onto the support surface until it forms a liquid bead. At a given bead size, i.e., volume, the tendency of liquid to flow onto the surface will be balanced by the hydrophobic surface interaction of the bead with the support surface, which acts to limit the total bead area on the surface, and by the surface tension of the droplet, which tends toward a given bead curvature. At this point, a given bead volume will have formed, and continued contact of the dispenser tip with the bead, as the dispenser tip is being withdrawn, will have little or no effect on bead volume.


The desired deposition volume, i.e., bead volume, formed is preferably in the range 2 pl (picoliters) to 2 nl (nanoliters), although volumes as high as 100 nl or more may be dispensed. It will be appreciated that the selected dispensed volume will depend on (i) the “footprint” of the dispenser tip(s), i.e., the size of the area spanned by the tip(s), (ii) the hydrophobicity of the support surface, and (iii) the time of contact with and rate of withdrawal of the tip(s) from the support surface. In addition, bead size may be reduced by increasing the viscosity of the medium, effectively reducing the flow time of liquid from the dispensing device onto the support surface. The drop size may be further constrained by depositing the drop in a hydrophilic region surrounded by a hydrophobic grid pattern on the support surface.


At a given tip size, bead volume can be reduced in a controlled fashion by increasing surface hydrophobicity, reducing time of contact of the tip with the surface, increasing rate of movement of the tip away from the surface, and/or increasing the viscosity of the medium. Once these parameters are fixed, a selected deposition volume in the desired picoliter to nanoliter range can be achieved in a repeatable fashion.


After depositing a liquid droplet of a Bacillus licheniformis gene sample at one selected location on a support, the tip may be moved to a corresponding position on a second support, the Bacillus licheniformis gene sample is deposited at that position, and this process is repeated until the random nucleic acid fragment sample has been deposited at a selected position on a plurality of supports.


This deposition process may then be repeated with another random nucleic acid fragment sample at another microarray position on each of the supports.


The diameter of each Bacillus licheniformis gene region is preferably between about 20-200 μm. The spacing between each region and its closest (non-diagonal) neighbor, measured from center-to-center, is preferably in the range of about 20-400 μm. Thus, for example, an array having a center-to-center spacing of about 250 μm contains about 40 regions/cm or 1,600 regions/cm2. After formation of the array, the support is treated to evaporate the liquid of the droplet forming each region, to leave a desired array of dried, relatively flat Bacillus licheniformis gene or oligo thereof regions. This drying may be done by heating or under vacuum. The DNA can also be UV-crosslinked to the polymer coating.


Nucleic Add Probes. In the methods of the present invention, the strains are cultivated in a nutrient medium with and without a substrate using methods well known in the art for isolation of nucleic acids to be used as probes. For example, the strains may be cultivated by shake flask cultivation, small-scale or large-scale fermentation (including continuous, batch, fed-batch, or solid state fermentations) in laboratory or industrial fermentors performed in a suitable medium. The cultivation takes place in a suitable nutrient medium comprising carbon and nitrogen sources and inorganic salts, using procedures known in the art. Suitable media are available from commercial suppliers or may be prepared according to published compositions (e.g., in catalogues of the American Type Culture Collection).


The nucleic acid probes from the microbial strains cultured on medium with and without substrate may be any nucleic acid including genomic DNA, cDNA, and RNA, and may be isolated using standard methods known in the art.


The populations of isolated nucleic acid probes may be labeled with detection reporters such as colorimetric, radioactive for example, 32P, 33P, or 35S), fluorescent reporters, or other reporters using methods known in the art (Chen et al., 1998, Genomics 51: 313-324; DeRisi at al., 1997, Science 278: 680-686; U.S. Pat. No. 5,770,367).


In a preferred aspect, the probes are labeled with fluorescent reporters. For example, the DNA probes may be labeled during reverse transcription from the respective RNA pools by incorporation of fluorophores as dye-labeled nucleotides (DeRisi et al., 1997, supra), e.g., Cy5-labeled deoxyuridine triphosphate, or the isolated cDNAs may be directly labeled with different fluorescent functional groups. Fluorescent-labeled nucleotides include, but are not limited to, fluorescein conjugated nucleotide analogs (green fluorescence), lissamine nucleotide analogs (red fluorescence). Fluorescent functional groups include, but are not limited to, Cy3 (a green fluorescent dye) and Cy5 (red fluorescent dye).


Array Hybridization. The labeled nucleic acids from the two strains cultivated with and without substrate are then added to an array of Bacillus licheniformis genes under conditions where the nucleic acid pools from the two strains hybridize to complementary sequences of the Bacillus licheniformis genes on the array. For purposes of the present invention, hybridization indicates that the labeled nucleic acids from the two strains hybridize to the Bacillus licheniformis genes under very low to very high stringency conditions.


A small volume of the labeled nucleic acids mixture is loaded onto the substrate. The solution will spread to cover the entire microarray. In the case of a multi-cell substrate, one or more solutions are loaded into each cell which stop at the barrier elements.


For nucleic add probes of at least about 100 nucleotides in length, microarray hybridization conditions described by Eisen and Brown, 1999, Methods of Enzymology 303: 179-205, may be used. Hybridization is conducted under a cover slip at 65° C. in 3×SSC for 4-16 hours followed by post-hybridization at room temperature after removal of the cover slip in 2×SSC, 0.1% SDS by washing the array two or three times in the solution, followed by successive washes in 1×SSC for 2 minutes and 0.2×SSC wash for two or more minutes.


Conventional conditions of very low to very high stringency conditions may also be used. Very low to very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and either 25% formamide for very low and low stringencies, 35% formamide for medium and medium-high stringencies, or 50% formamide for high and very high stringencies, following standard Southern blotting procedures.


The carrier material is finally washed three times each for 15 minutes using 2×SSC, 0.2% SDS preferably at least at 45° C. (very low stringency), more preferably at east at 50° C. (low stringency), more preferably at least at 55° C. (medium stringency), more preferably at least at 60° C. (medium-high stringency), even more preferably at least at 65° C. (high stringency), and most preferably at least at 70° C. (very high stringency).


For shorter nucleic acid probes which are less than 50 nucleotides, microarray hybridization conditions described by Kane et al., 2000, Nucleic Acids Research 28: 4552-4557, may be used. Hybridization is conducted under a supported coverslip at 42° C. for 16-18 hours at high humidity in 50% formamide, 4.1×Denhardt's solution, 4.4×SSC, and 100 μg/ml of herring sperm DNA. Arrays are washed after removal of the coverslip in 4×SSC by immersion into 1×SSC, 0.1% SDS for 10 minutes, 0.1×SSC, 0.1% SDS twice for 10 minutes, and 0.1×SSC twice for 10 minutes.


For shorter nucleic acid probes which are about 50 nucleotides to about 100 nucleotides in length, conventional stringency conditions may be used. Such stringency conditions are defined as prehybridization, hybridization, and washing post-hybridization at 5° C. to 10° C. below the calculated Tm using the calculation according to Bolton and McCarthy (1962, Proceedings of the National Academy of Sciences USA 48:1390) in 0.9 M NaCl, 0.09 M Tris-HCl pH 7.6, 6 mM EDTA, 0.5% NP-40, 1×Denhardt's solution, 1 mM sodium pyrophosphate, 1 mM sodium monobasic phosphate, 0.1 mM ATP, and 0.2 mg of yeast RNA per ml following standard Southern blotting procedures.


The carrier material is finally washed once in 6×SSC plus 0.1% SDS for 15 minutes and twice each for 15 minutes using 6×SSC at 5° C. to 10° C. below the calculated Tm.


The choice of hybridization conditions will depend on the degree of homology between the Bacillus licheniformis genes and the nucleic acid probes obtained from the strain cultured with and without inducing substrate. For example, where the nucleic acid probes and the Bacillus licheniformis genes are obtained from identical strains, high stringency conditions may be most suitable. Where the strains are from a genus or species different from which the Bacillus licheniformis genes were obtained, low or medium stringency conditions may be more suitable.


In a preferred aspect, the hybridization is conducted under low stringency conditions. In a more preferred aspect, the hybridization is conducted under medium stringency conditions. In a most preferred aspect, the hybridization is conducted under high stringency conditions.


The entire solid support is than reacted with detection reagents if needed and analyzed using standard colorimetric, radioactive, or fluorescent detection means. All processing and detection steps are performed simultaneously to all of the microarrays on the solid support ensuring uniform assay conditions for all of the microarrays on the solid support.


Detection. The most common detection method is laser-induced fluorescence detection using confocal optics (Cheung et al., 1998, Nat. Genet. 18: 225-230). The array is examined under fluorescence excitation conditions such that (i) the Bacillus licheniformis genes on the array that hybridize to the first nucleic acid probes obtained from the strain cultured without inducing substrate and to the second nucleic acid probes obtained from the strain cultured with inducing substrate produce a distinct first fluorescence emission color and a distinct second fluorescence emission color, respectively, and (ii) the Bacillus licheniformis genes on the array that hybridize to substantially equal numbers of nucleic acid probes obtained from the strain cultured without inducing substrate and from the strain cultured with inducing substrate produce a distinct combined fluorescence emission color; wherein the relative expression of the genes in the strains can be determined by the observed fluorescence emission color of each spot on the array.


The fluorescence excitation conditions are based on the selection of the fluorescence reporters. For example, Cy3 and Cy5 reporters are detected with solid state lasers operating at 532 nm and 632 nm, respectively.


However, other methods of detection well known in the art may be used such as standard photometric, calorimetric, or radioactive detection means, as described earlier.


Data Analysis. The data obtained from the scanned image may then be analyzed using any of the commercially available image analysis software. The software preferably identifies array elements, subtracts backgrounds, deconvolutes multi-color images, flags or removes artifacts, verifies that controls have performed properly, and normalizes the signals (Chen et al., 1997, Journal of Biomedical Optics 2: 364-374).


Several computational methods have been described for the analysis and interpretation of microarray-based expression profiles including cluster analysis (Eisen at al., 1998, Proc. Nat Acad. Sci. USA 95: 14863-14868), parametric ordering of genes (Spellman et al., 1998, Mol. Biol. Cell 9: 3273-3297), and supervised clustering methods based on representative hand-picked or computer-generated expression profiles (Chu at al., 1998. Science 282: 699-705). Preferred methods for evaluating the results of the microarrays employ statistical analysis to determine the significance of the differences in expression levels. In the methods of the present invention, the difference in the detected expression level is at least about 10% or greater, preferably at least about 20% or greater, more preferably at least about 50% or greater, even more preferably at least about 75% or greater; and most preferably at least about 100% or greater.


One such preferred system is the Significance Analysis of Microarrays (SAM) (Tusher et al., 2001, Proc. Natl. Acad. Sci. USA 98: 5116-5121). Statistical analysis allows the determination of significantly altered expression of levels of about 50% or even less. The PAM (or predictive analysis for microarrays) represents another approach for analyzing the results of the microarrays (Tibshirani at al., 2002, Proc. Natl. Acad. Sci. USA 99: 6567-6572).


Cluster algorithms may also be used to analyze microarray expression data. From the analysis of the expression profiles it is possible to identify co-regulated genes that perform common metabolic or biosynthetic functions. Hierarchical clustering has been employed in the analysis of microarray expression data in order to place genes into clusters based on sharing similar patterns of expression (Eisen at al., 1998, supra). This method yields a graphical display that resembles a kind of phylogenetic tree where the relatedness of the expression behavior of each gene to every other gene is depicted by branch lengths. The programs Cluster and TreeView, both written by Michael Eisen (Eisen et al., 1998 Proc. Nat. Acad. Sci. USA 95: 14863-14868) are freely available. Genespring is a commercial program available for such analysis (Silicon Genetics, Redwood City, Calif.).


Self-organizing maps (SOMs), a non-hierarchical method, have also been used to analyze microarray expression data (Tamayo et al., 1999, Proc. Natl. Acad. Sri. USA 96: 2907-2912). This method involves selecting a geometry of nodes, where the number of nodes defines the number of clusters. Then, the number of genes analyzed and the number of experimental conditions that were used to provide the expression values of these genes are subjected to an iterative process (20,000-50,000 iterations) that maps the nodes and data points into multidimensional gene expression space. After the identification of significantly regulated genes, the expression level of each gene is normalized across experiments. As a result, the expression profile of the genome is highlighted in a manner that is relatively independent of each gene's expression magnitude. Software for the “GENECLUSTER” SOM program for microarray expression analysis can be obtained from the Whitehead/MIT Center for Genome Research. SOMs can also be constructed using the GeneSpring software package.


Isolation of Genes. Probes containing genes or portions thereof identified to be induced by the present of substrate in the medium are characterized by determining the sequence of the probe. Based on the sequence, the gene can then be isolated using methods well known in the art.


The techniques used to isolate or clone a gene include isolation from genomic DNA, preparation from cDNA, or a combination thereof. The cloning of the gene from such genomic DNA can be effected, e.g., by using the well known polymerase chain reaction (PCR) or antibody screening of expression libraries to detect cloned DNA fragments with shared structural features. See, e.g., Innis at al., 1990, PCR: A Guide to Methods and Application, Academic Press, New York. Other nucleic acid amplification procedures such as ligase chain reaction (LCR), ligated activated transcription (LAT) and nucleic acid sequence-based amplification (NASBA) may be used. The gene may be cloned from the strain of interest, or another or related organism and thus, for example, may be an allelic or species variant of the gene.


Methods for Monitoring Differential Expression of a Plurality of Genes

The present invention also relates to methods for monitoring differential expression of a plurality of genes in a first bacterial cell relative to expression of the same genes in one or more second bacterial cells, comprising:


(a) adding a mixture of detection reporter-labeled nucleic acids isolated from the bacterial cells to a substrate containing an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198, under conditions where the detection reporter-labeled nucleic acids hybridize to complementary sequences of the Bacillus licheniformis genes on the array, wherein the nucleic acids from the first bacterial cell and the one or more second bacterial cells are labeled with a first detection reporter and one or more different second detection reporters, respectively; and


(b) examining the array under conditions wherein the relative expression of the genes in the bacterial cells is determined by the observed detection signal of each spot on the array in which (i) the Bacillus licheniformis genes on the array that hybridize to the nucleic acids obtained from either the first or the one or more second bacterial cells produce a distinct first detection signal or one or more second detection signals, respectively, and (ii) the Bacillus licheniformis genes on the array that hybridize to the nucleic acids obtained from both the first and one or more second bacterial produce a distinct combined detection signal.


The methods of the present invention may be used to monitor global expression of a plurality of genes from a Bacillus cell, discover new genes, identify possible functions of unknown open reading frames, and monitor gene copy number variation and stability. For example, the global view of changes in expression of genes may be used to provide a picture of the way in which Bacillus cells adapt to changes in culture conditions, environmental stress, or other physiological provocation. Other possibilities for monitoring global expression include spore morphogenesis, recombination, metabolic or catabolic pathway engineering.


The methods of the present invention are particularly advantageous since one spot on an array equals one gene or open reading frame because extensive follow-up characterization is unnecessary since sequence information is available, and the Bacillus licheniformis microarrays can be organized based on function of the gene products.


Microarrays. Methods for preparing the microarrays are described herein.


Bacterial Cells. In the methods of the present invention, the two or more Bacillus cells may be any Bacillus cell where one of the cells is used as a reference for identifying differences in expression of the same or similar complement of genes in the other cell(s). In one aspect, the two or more cells are the same cell. For example, they may be compared under different growth conditions, e.g., oxygen limitation, nutrition, and/or physiology. In another aspect, one or more cells are mutants of the reference cell. For example, the mutant(s) may have a different phenotype. In a further aspect, the two or more cells are of different species (e.g., Bacillus clausii and Bacillus subtilis). In another further aspect, the two or more cells are of different genera. In an even further aspect, one or more cells are transformants of the reference cell, wherein the one or more transformants exhibit a different property. For example, the transformants may have an improved phenotype relative to the reference cell and/or one of the other transformants. The term “phenotype” is defined herein as an observable or outward characteristic of a cell determined by its genotype and modulated by its environment. Such improved phenotypes may include, but are not limited to, improved secretion or production of a protein or compound, reduced or no secretion or production of a protein or compound, improved or reduced expression of a gene, desirable morphology, an altered growth rate under desired conditions, relief of over-expression mediated growth inhibition, or tolerance to low oxygen conditions.


The Bacillus cells may be any Bacillus cells, but preferably Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus cereus, Bacillus circulans, Bacillus clausii, Bacillus coagulans, Bacillus fastidiosus, Bacillus firmus, Bacillus lautus, Bacillus lentus, Bacillus licheniformis, Bacillus macerans, Bacillus megaterium, Bacillus methanolicus, Bacillus pumilus, Bacillus sphaericus, Bacillus stearothermophilus, Bacillus subtilis, or Bacillus thuringiensis cells.


In a preferred aspect, the Bacillus cells are Bacillus alkalophilus cells. In another preferred aspect. The Bacillus cells are Bacillus amyloliquefaciens cells. In another preferred aspect, the Bacillus cells are Bacillus brevis cells. In another preferred aspect, the Bacillus cells are Bacillus cereus cells. In another preferred aspect, the Bacillus cells are Bacillus circulans cells. In another preferred aspect, the Bacillus cells are Bacillus clausii cells. In another preferred aspect, the Bacillus cells are Bacillus coagulans cells. In another preferred aspect, the Bacillus cells are Bacillus fastidiosus cells. In another preferred aspect, the Bacillus cells are Bacillus firmus cells. In another preferred aspect, the Bacillus cells are Bacillus lautus cells. In another preferred aspect, the Bacillus cells are Bacillus lentus cells. In another preferred aspect, the Bacillus cells are Bacillus licheniformis cells. In another preferred aspect, the Bacillus cells are Bacillus macerans cells. In another preferred aspect, the Bacillus cells are Bacillus megaterium cells. In another preferred aspect, the Bacillus cells are Bacillus methanolicus cells. In another preferred aspect, the Bacillus cells are Bacillus pumilus cells. In another preferred aspect, the Bacillus cells are Bacillus sphaericus cells. In another preferred aspect, the Bacillus cells are Bacillus stearothermophilus cells. In another preferred aspect, the Bacillus cells are Bacillus subtilis cells. In another preferred aspect, the Bacillus cells are Bacillus thuringiensis cells.


In a more preferred aspect, the Bacillus cells are Bacillus licheniformis cells. In a most preferred aspect, the Bacillus licheniformis cells are Bacillus licheniformis ATCC 14580 cells.


In another more preferred aspect, the Bacillus cells are Bacillus clausii cells. In another most preferred aspect, the Bacillus clausii cells are Bacillus clausii NCIB 10309 cells.


It will be understood that the term “Bacillus” also encompasses relatives of Bacillus such as Paenibacillus, Oceanobacillus, and the like.


In the methods of the present invention, the cells are cultivated in a nutrient medium suitable for growth using methods well known in the art for isolation of the nucleic acids to be used as probes. For example, the cells may be cultivated by shake flask cultivation, small-scale or large-scale fermentation (including continuous, batch, fed-batch, or solid state fermentations) in laboratory or industrial fermentors performed in a suitable medium. The cultivation takes place in a suitable nutrient medium comprising carbon and nitrogen sources and inorganic salts, using procedures known in the art. Suitable media are available from commercial suppliers or may be prepared according to published compositions (e.g., in catalogues of the American Type Culture Collection).


Nucleic Acid Probes. The nucleic acid probes from the two or more Bacillus cells may be any nucleic acid including genomic DNA, cDNA, and RNA, and may be isolated using standard methods known in the art, as described herein. The populations of isolated nucleic acid probes may be labeled with colorimetric, radioactive, fluorescent reporters, or other reporters using methods described herein.


In a preferred aspect, the probes are labeled with fluorescent reporters, e.g., Cy3 (a green fluorescent dye) and Cy5 (red fluorescent dye), as described herein.


Array Hybridization. The labeled nucleic acids from the two or more Bacillus cells are then added to a substrate containing an array of Bacillus licheniformis genes under conditions, as described herein, where the nucleic acid pools from the two or more Bacillus cells hybridize to complementary sequences of the Bacillus licheniformis genes on the array.


Detection and Data Analysis. The same methods as described herein are used for detection and data analysis.


Computer Readable Media and Computer-Based Systems

The Bacillus licheniformis chromosome and its genes described herein may be “provided” in a variety of media to facilitate their use. The term “provided” refers to a manufacture comprising an array of Bacillus licheniformis genes. Such manufactures provide the Bacillus licheniformis genes in a form which allows one skilled in the art to examine the manufacture using means not directly applicable to examining the chromosome or a subset thereof as it exists in nature or in purified form.


Thus, the present invention also relates to such a manufacture in the form of a computer readable medium comprising an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198.


In one application of this aspect, the Bacillus licheniformis genes of the present invention can be recorded on computer readable media. The term “computer readable media” is defined herein as any medium which can be read and accessed by a computer. Such computer readable media include, but are not limited to, magnetic storage media, e.g., floppy discs, hard disc storage medium, and magnetic tape; optical storage media, e.g., CD-ROM, DVD; electrical storage media, e.g., RAM and ROM; and hybrids of these categories, e.g., magnetic/optical storage media. One skilled in the art can readily appreciate how any of the presently known computer readable media can be used to create a manufacture comprising computer readable medium having recorded thereon a nucleotide sequence of the present invention. Likewise, it will be clear to those of skill how additional computer readable media that may be developed also can be used to create analogous manufactures having recorded thereon a nucleotide sequence of the present invention.


As used herein, “recorded” refers to a process for storing information on computer readable medium. One skilled in the art can readily adopt any of the presently known methods for recording information on computer readable medium to generate manufactures comprising the nucleotide sequence information of the present invention.


A variety of data storage structures are available for creating a computer readable medium having recorded thereon a nucleotide sequence of the present invention. The choice of the data storage structure will generally be based on the means chosen to access the stored information. In addition, a variety of data processor programs and formats can be used to store the nucleotide sequence information of the present invention on computer readable medium. The sequence information can be represented in a word processing text file, formatted in commercially-available software such as WordPerfect and Microsoft Word, or represented in the form of an ASCII file, stored in a database application, such as DB2, Sybase, Oracle, or the like. A skilled artisan can readily adapt any number of data-processor structuring formats (e.g., text file or database) in order to obtain computer readable medium having recorded thereon the nucleotide sequence information of the present invention.


Various computer software programs are publicly available that allow a skilled artisan to access sequence information provided in a computer readable medium. Thus, by providing in computer readable form an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198, enables one skilled in the art to routinely access the provided sequence information for a wide variety of purposes.


Software utilizing the BLAST (Altschul et al., 1990, supra), BLAZE (Brutlag et al., 1993, Comp. Chem. 17: 203-207), GENEMARK (Lukashin and Borodovsky, 1998, Nucleic Acids Research 26: 1107-1115), GENSCAN (Burge and Karlin, 1997, Journal of Molecular Biology 268: 78-94), GLIMMER (Salzberg et al., 1998, Nucleic Acids Research 26: 544-548), and GRAIL (Xu et al., 1994, Comput. Appl. Biosci. 10: 613-623) search algorithms may be used to identify open reading frames (ORFS) within a genome of interest, which contain homology to ORFs or proteins from both Bacillus licheniformis and Bacillus clausii and from other organisms. Among the ORFs discussed herein are protein encoding fragments of the Bacillus licheniformis and Bacillus clausii genomes useful in producing commercially important proteins, such as enzymes used in fermentation reactions and in the production of commercially useful metabolites.


The present invention further provides systems, particularly computer-based systems, which contain the sequence information described herein. Such systems are designed to identify, among other things, genes and gene products—many of which could be products themselves or used to genetically modify an industrial expression host through increased or decreased expression of a specific gene sequence(s).


The term “a computer-based system” is herein defined as the hardware means, software means, and data storage means used to analyze the nucleotide sequence information of the present invention. The minimum hardware means of the computer-based systems of the present invention comprises a central processing unit (CPU), input means, output means, and data storage means. One skilled in the art can readily appreciate that any currently available computer-based system is suitable for use in the present invention.


As stated above, the computer-based systems of the present invention comprise a data storage means having stored therein a nucleotide sequence of the present invention and the necessary hardware means and software means for supporting and implementing a search means.


The term “data storage means” is defined herein as memory which can store nucleotide sequence information of the present invention, or a memory access means which can access manufactures having recorded thereon the nucleotide sequence information of the present invention.


The term “search means” refers is defined herein as one or more programs which are implemented on the computer-based system to compare a target sequence or target structural motif with the sequence information stored within the data storage means. Search means are used to identify fragments or regions of the present genomic sequences which match a particular target sequence or target motif. A variety of known algorithms are disclosed publicly and a variety of commercially available software for conducting search means are and can be used in the computer-based systems of the present invention. Examples of such software includes, but is not limited to, MacPattern (Fuchs, 1991, Comput. Appl. Biosci. 7: 105-106), BLASTN and BLASTX National Center for Biotechnology Information (NCBI). One skilled in the art can readily recognize that any one of the available algorithms or implementing software packages for conducting homology searches can be adapted for use in the present computer-based systems.


The term “target sequence” is defined here as any DNA (genomic DNA, cDNA) or amino acid sequence of six or more nucleotides or two or more amino acids. One skilled in the art can readily recognize that the longer a target sequence is, the less likely a target sequence will be present as a random occurrence in the database. The most preferred sequence length of a target sequence is from about 10 to 100 amino acids or from about 30 to 300 nucleotide residues. However, it is well recognized that searches for commercially important fragments, such as sequence fragments involved in gene expression and protein processing, may be of shorter length.


The term “a target structural motif” or “target motif” is defined herein as any rationally selected sequence or combination of sequences in which the sequence(s) are chosen based on a three-dimensional configuration which is formed upon the folding of the target motif. There are a variety of target motifs known in the art. Protein target motifs include, but are not limited to, enzyme active sites and signal sequences, substrate and cofactor binding domains, transmembrane domains, and sites for post-translational modifications. Nucleic acid target motifs include, but are not limited to, promoter sequences, hairpin structures and inducible expression elements (protein binding sequences), repeats, palindromes, dyad symmetries, and transcription and translation start and stop sites.


A variety of structural formats for the input and output means can be used to input and output the information in the computer-based systems of the present invention. A preferred format for an output means ranks fragments of the Bacillus licheniformis or Bacillus clausii genomic sequences possessing varying degrees of homology to the target sequence or target motif. Such presentation provides one skilled in the art with a ranking of sequences which contain various amounts of the target sequence or target motif and identifies the degree of homology contained in the identified fragment.


A variety of comparing means can be used to compare a target sequence or target motif with the data storage means to identify sequence fragments of the Bacillus licheniformis and Bacillus clausii genomes. For example, implementing software which utilize the BLAST and BLAZE algorithms, described in Altschul at al., 1990, supra, may be used to identify open reading frames within the Bacillus licheniformis or Bacillus clausii genome or the genomes of other organisms. A skilled artisan can readily recognize that any one of the publicly available homology search programs can be used as the search means for the computer-based systems of the present invention. Suitable proprietary systems that may be known to those of skill also may be employed in this regard.


Codon Usage Tables

The present invention further relates to methods for preparing a synthetic gene, comprising (a) generating a codon usage table based on codons used in one or more open reading frames or portions thereof of SEQ ID NO: 1, (b) constructing a synthetic gene or portion thereof that contains in place of one or more native codons one or more preferred codons from the codon usage table, and (c) recovering the synthetic gene. In a preferred aspect, the codon usage table is Table 4 and/or Table 5.


The Bacillus licheniformis chromosomal sequence of SEQ ID NO: 1 or portions thereof can be used to generate codon usage tables to design synthetic genes for their efficient heterologous expression in Bacillus licheniformis host cells. The codon usage tables can be based on (1) the codon used in all the open reading frames, (2) selected open reading frames, (3) fragments of the open reading frames, or (4) fragments of selected open reading frames. With a codon usage table, synthetic genes can be designed with only the most preferred codon for each amino acid; with a number of common codons for each amino acid; or with the same or a similar statistical average of codon usages found in the table of choice.


The synthetic gene can be constructed using any method such as site-directed mutagenesis or PCR generated mutagenesis in accordance with methods known in the art. Although, in principle, the modification may be performed in vivo, i.e., directly on the cell expressing the nucleotide sequence to be modified, it is preferred that the modification is performed in vitro.


The synthetic gene can be further modified by operably linking the synthetic gene to one or more control sequences which direct the expression of the coding sequence in a suitable host cell under conditions compatible with the control sequences using the methods described herein. Nucleic acid constructs, recombinant expression vectors, and recombinant host cells comprising the synthetic gene can also be prepared using the methods described herein.


The present invention also relates to methods for producing a polypeptide encoded by such a synthetic gene comprising (a) cultivating a host cell comprising the synthetic gene under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.


The present invention is further described by the following examples which should not be construed as limiting the scope of the invention.


EXAMPLES
Example 1
Shotgun DNA Sequencing and Genome Assembly

The genome of the type strain Bacillus licheniformis ATCC 14580 was sequenced by a combination of the whole genome shotgun method described by Wilson, R. K. and Mardis, E. R., 1997, In Genome Analysis: A Laboratory Manual, Vol. 1, eds. Birren, B., Green, E. D., Meyers, R. M., and Roskams, J. (Cold Spring Harbor Press, Cold Spring Harbor, N.Y.), pp. 397-454, and fosmid end sequencing (Kim, U. J., Shizuya, H., de Jong, P. J., Birren, B. and Simon, M. I., 1992, Nucleic Acids Res. 20: 1083-1085; Longmire, J. L. and Brown, N. C., 2003, Biotechniques 35: 50-54; Zhao, S., Malek, J., Mahairas, G., Fu, L., Nierman, W., Venter, J. C., and Adams, M. D., 2000, Genomics 63: 321-332).


Genomic DNA of Bacillus licheniformis ATCC 14580 was isolated using the following method; A single colony was used to inoculate 20 ml of LB broth (Davis, R. W., Botstein, D., and Roth, J. R. 1980, Advanced Bacterial Genetics, Cold Spring Harbor Press, Cold Spring Harbor, N.Y.) in a sterile 125 ml Erlenmeyer flask. The culture was incubated at 37° C. overnight with agitation at 240 rpm. The resulting cells were collected by centrifugation in a 45 ml Oak Ridge tube for 10 minutes at 6000×g, and the cell pellet was resuspended in 5 ml of Tris-glucose buffer (50 mM Tris-HCl, pH 8.0, 50 mM glucose, 10 mM EDTA). Lysozyme was added to a final concentration of 50 μg/ml and the suspension was incubated in a 37° C. water bath for 25 minutes. Next, 200 μl of 10% SDS was added and the tubes were gently inverted several times. Five milliliters of a second detergent mixture (1% Brij, 1% deoxycholate, 50 mM EDTA, pH 7.5) was added, and the tubes were inverted several times while incubating for 20 minutes at room temperature. An equal volume of phenol:chloroform (1:1 v/v) was added and the tubes were inverted gently at room temperature for 20-30 minutes. The tubes were centrifuged for 20 minutes at 12,000×g, 4° C. The top aqueous layer was carefully removed with a wide-bore pipette and placed in a clean 45 ml Oak Ridge tube. The phenol:chloroform extraction was repeated and 1/10 volume of 3 M sodium acetate pH 5.2 was added to the aqueous layer. Two volumes of cold ethanol were carefully layered on top and the DNA was spooled from the solution onto a sterile glass rod. Spooled DNA was carefully rinsed in 70% ethanol and resuspended in a suitable amount of TE buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA).


Plasmid libraries were constructed using randomly-sheared and BamHI-digested genomic DNA that was enriched for 2-3 kb fragments by preparative agarose gel electrophoresis (Berka, R. M., Schneider, P., Golightly, E. J., Brown, S. H., Madden, M., Brown, K. M., Halkier, T., Mondorf, K., and Xu, F., 1997, Appl. Environ. Microbiol. 63: 3151-3157). Approximately 49,000 random clones were sequenced using dye-terminator chemistry (Applied Biosystems, Foster City, Calif.) with ABI 377 and ABI 3700 automated sequencers yielding approximately 6× coverage of the genome. A combination of methods was employed for gap closure including sequencing on fosmids (Kim, U. J., Shizuya, H., de Jong, P. J., Birren, B., and Simon, M. I., 1992, Nucleic Acids Res. 20: 1083-1085), primer walking on selected clones, and PCR-amplified DNA fragments. Fosmid libraries were constructed using a commercial kit from Epicentre (Madison, Wis.). Data from both ends of approximately 1975 fosmid clones with an average insert size of 40 kb were incorporated to aid in validation of the final assembly. In total, the number of input reads was 62,685 with 78.6% of these incorporated into the final assembly. Sequences were base called using TraceTuner 2.0 (Paracel, Inc., Pasadena, Calif.) and assembled using the Paracel Genome Assembler (Paracel, Inc., Pasadena, Calif.) with optimized parameters and the quality score set to >20. Phrap, Crossmatch, and Consed were used for sequence finishing (Gordon D., Abajian C., and Green P., 1998, Genome Res. 8: 195-202).


Example 2
Identification and Annotation of Open Reading Frames (ORFs)

Protein coding regions in the assembled genome sequence data were identified using EasyGene (Larsen, T. S., and Krogh, A., 2003, BMC Bioinformatics 4: 21), Glimmer (Delcher, A. L., Harmon, D., Kasif, S., White, O. and Salzberg, S. L., 1999, Nucleic Acids Res. 27, 4636-4641), and FrameD (Schiex, T., Gouzy, J., Moisan, A. and de Oliveira, Y., 2003, Nucleic Acids Res. 31, 3738-3741). Only EasyGene gene models with an R-value of less than 2 and log-odds score greater than −10 were used. Predicted proteins were compared to the non-redundant database PIR-NREF (Wu, C. H., Huang, H., Arminski, L., Castro-Alvear, J., Chen, Y., Hu, Z. Z., Ledley, R. S., Lewis, K. C., Mewes, H. W., Orcutt, B. C., 2002, Nucleic Acids Research 30: 35-37) and the Bacillus subtilis genome (SubtilList) using BLASTP with an E-value threshold of 10−5. InterProScan was used to predict putative function. (Zdobnov, E. M. and Apweiler, R., 2001, Bioinformatics 17, 847-848). The InterPro analysis included comparison to Pfam (Bateman, A., Coin, L., Durbin, R. Finn, R. D., Hollich, V., Griffiths-Jones, S., Khanna, A., Sonnhammer, E. L., et al., 2004, Nucleic Acids Res. 32, D138-D141), TIGRfam (Haft, D. J., Selengut, J. D. and White, O., 2003, Nucleic Acids Res. 31: 371-373), Interpro (Apweiler, R., Attwood, T. K., Bairock, A., Bateman, A., Birney, E., Biswas, M., Bucher, P., Cerutti, L., Corpet, F., Croning, M. D., et al., 2001, Nucleic Acids Res. 29: 37-40), signal peptide prediction using SignalP (Nielsen, H., Engelbrecht, J., Brunak, S., and von Heijne, G., 1997, Protein Engineering 10: 1-6), and trans-membrane domain prediction using TMHMM (Krogh, A., Larsson, B., von Heijne, G. and Sonnhammer, E. L. L., 2000, J. Mol. Biol. 305, 567-580). These ORFs were assigned to functional categories based on the Cluster of Orthologous Groups (COG) database (www.ncbi.nlm.nih.gov/COG) with manual verification as described (Tatusov, R. L., Koonin, E. V. and Lipman, D. J., 1997, Science 278: 631-637; Koonin, E. V. and Galperin, M. Y., 2002, Sequence-Evolution-Function: Computational Approaches in Comparative Genomics (Kluwer, Boston)). Transfer RNA genes were identified using tRNAscan-SE (Lowe, T. M. and Eddy, S. R., 1997, Nucleic Acids Res. 25: 955-964).


Example 3
General features of the Bacillus licheniformis Genome

The genome of Bacillus licheniformis ATCC 14580 was determined to consist of a circular molecule of 4,222,336 bp with an average GC content of 46.2% (Table 2). No plasmids were found during the genome analysis, and none were found by agarose gel electrophoresis.


The genome contains 4208 predicted protein-coding genes with an average size of 873 bp, seven rRNA operons, and 81 tRNA genes. Using a combination of several gene-finding algorithms 4208 protein coding ORFs were predicted. These ORFs constitute 87% of the genome and have an average length of 873 bp. Approximately 48% of the ORFs are encoded on one DNA strand and 52% on the other strand. Among the protein coding ORFs, 3948 (94%) have significant similarity to proteins in PIR, and 3187 of these gene models contain Interpro motifs and 2895 contain protein motifs found in PFAM. The number of hypothetical and conserved hypothetical proteins in the Bacillus licheniformis genome with hits in the FIR database was 1318 (212 conserved hypothetical ORFs). Among the list of hypothetical and conserved hypothetical ORFs, 683 (52%) have protein motifs contained in PFAM (148 conserved hypothetical ORFs). There are 72 tRNA genes representing all 20 amino acids and 7 rRNA operons.


The likely origin of replication was identified by similarities to several features of Bacillus subtilis origin (Moriya, S., Fukuoka, T., Ogasawara, N., and Yoshikawa, H., 1988, EMBO Journal 7: 2911-2917; Ogasawara, N., Nakai, S., and Yoshikawa, H., 1994, DNA Res. 1, 1-14; Kadoya, R., Hassan, A. K., Kasahara, Y., Ogasawara, N., and Moriya, S., 2002, Mol. Microbiol. 45: 73-87; Tosato, V., Gjuracic, K., Vlahovicek, K., Pongor, S., Danchin, A., and Bruschi, C. V., 2003, FEMS Microbiol, Lett. 218: 23-30). These included (a) colocalization of four genes (rpmH, dnaA, dnaN, and recF) found near the origin of the Bacillus subtilis chromosome, (b) GC nucleotide skew [(G−C)/(G+C)] analysis, and (c) the presence of multiple dnaA-boxes (Pedersen, A. G., Jensen, L. J., Brunak, S., Staerfeldt, H. H., and Ussery, D. W., 2000, Mol. Biol. 299: 907-930; Christensen, B. B., Atlung, T., and Hansen, F. G., 1999, J. Bacterial. 181: 2683-2688; Majka, J., Jakimowicz, D., Messer, W., Schrempf, H., Lisowski, M., and Zakrzewska-Czerwinska, J., 1999, Eur. J. Biochem. 260: 325-335) and AT-rich sequences in the region immediately upstream of the dnaA gene. On the basis of these observations, a cytosine residue of the BstBI restriction site was assigned between the rpmH and dnaA genes to be the first nucleotide of the Bacillus licheniformis genome. The replication termination site was localized near 2.02 Mb by GC skew analysis. This region lies roughly opposite the origin of replication.


Unlike Bacillus subtilis, no apparent gene encoding a replication terminator protein (rtp) was found in Bacillus licheniformis. The Bacillus halodurans genome also lacks an rtp function (Takami, H., Nakasone, K., Takaki, Y., Maeno, G., Sasaki, R., Masui, N., Fuji, F., Hirama, C., Nakamura, Y., Ogasawara, N. et al., 2000, Nucleic Acids Res. 28: 4317-4331), and it seems likely that Bacillus subtilis acquired the rtp gene following its divergence from Bacillus halodurans and Bacillus licheniformis.


Transposable elements and prophages. The genome of Bacillus licheniformis ATCC 14580 was determined to contain nine identical copies of a 1285 bp insertion sequence element termed IS3Bli1 (Lapidus, A., Galleron, N., Andersen, J. T., Jørgensen, P. L. Ehrlich, S. D., and Sorokin, A., 2002, FEMS Microbiol. Lett. 209: 23-30). This sequence shares a number of features with other IS3 family elements including direct repeats of three to five bp, a ten bp left inverted repeat, and a nine by right inverted repeat. IS3Bli1 encodes two predicted overlapping ORFs, designated orfA and orfB in relative translational reading frames of 0 and −1. The presence of a “slippery heptamer” motif, AAAAAAG, before the stop codon in orfA suggests that programmed translational frameshifting occurs between these two ORFs, resulting in a single gene product (Farabaugh, P., 1996, Microbiol. Rev. 60: 103-134). The orfB gene product harbors the DD[35]E[7]K motif, a highly conserved pattern among insertion sequences. Eight of the IS3Bli1 elements lie in intergenic regions, and one interrupts the comP gene. In addition to these insertion sequences, the genome encodes a putative transposase that is most closely related (E=1.8e-11) to one identified in the Thermoanaerobacter tengcongensis genome (Bao, Q., Tian, Y., Li, W., Xu, Z.; Xuan, Z., Hu, S., Dong, W., Yang, J., Chen, Y., Xue, Y., et al., 2002, Genome Res. 12: 689-700), however, similar genes are also found in the chromosomes of Bacillus halodurans (Takaki, H., Nakasone, K., Takaki, Y., Maeno, G., Sasaki, R., Masui, N., Fuji, F., Hirama, C., Nakamura, Y., Ogasawara, N. et al., 2000, Nucleic Acids Res. 28: 4317-4331), Oceanobacillus iheyensis (Takagi, H. Takaki, Y., and Uchiyama, I., 2002, Nucleic Acids Res. 30: 3927-3935.), Streptococcus agalactiae (Takahashi, S., Detrick, S., Whiting, A. A, Blaschke-Bonkowksy, A. J., Aoyagi, Y., Adderson, E. E., and Bohnsack, J. F., 2002, J. Infect. Dis. 186: 1034-1038), and Streptococcus pyogenes (Smoot, J. C., Barbian, K. D., Van Gompel, J. J., Smoot, L. M., Chaussee, M. S., Sylva, G. L., Sturdevant, D. E., Ricklefs, S. M., Porcella, S. F., Parkins, L. D., et al., 2002, Proc. Natl. Acad. Sci. U.S.A. 99: 4668-4673).


The presence of several bacteriophage lysogens or prophage-like elements was revealed by Smith-Waterman comparisons to other bacterial genomes and by their AT-rich signatures. Prophage sequences, designated NZP1 and NZP3 (similar to PBSX and φ-105, respectively), were uncovered by noting the presence of nearby genes encoding the large subunit of terminase, a signature protein that is highly conserved among prophages (Casjens, S., 2003, Mol. Microbiol. 49: 277-300). A terminase gene was not observed in a third putative prophage, termed NZP2 (similarity to SPP1), however, its absence may be the result of genome deterioration during evolution. Regions were observed in which the GC content is less than 39% usually encoded proteins that have no Bacillus subtilis orthologue and share identity only to hypothetical and conserved hypothetical genes. Two of these AT-rich segments correspond to the NZP2 and NZP3 prophages.


An isochore plot also revealed the presence of a region with an atypically high (62%) G+C content. This segment contains two hypothetical ORFs whose sizes (3831 and 2865 bp) greatly exceed the size of an average gene in Bacillus licheniformis. The first protein encodes a protein of 1277 amino acids for which Interpro predicted 16 collagen triple helix repeats, and the amino acid pattern TGATGPT is repeated 75 times within the polypeptide. The second ORF is smaller, and encodes a protein with 11 collagen triple helix repeats, and the same TGATGPT motif recurs 56 times. Interestingly, the chromosomal region (19 kb) adjacent to these genes is clearly non-colinear with the Bacillus subtilis genome, and virtually all of the predicted ORFs are hypothetical or conserved hypothetical proteins. There are a number of bacterial proteins listed in PIR that contain collagen triple helix repeat regions including two from Mesorhizobium loti (accession numbers NF00607049 and NF00607035) and three from Bacillus cereus (accession numbers NF01692528, NF01269899, and NF01694666). These putative orthologs share 53-76% amino acid sequence identity with their counterparts in Bacillus licheniformis, although their functions are unknown.


Extracellular enzymes. In the Bacillus licheniformis genome, 689 of the 4208 gene models have signal peptides as forecasted by SignalP (Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites, Henrik Nielsen, Jacob Engelbrecht, Søren Brunak and Gunnar von Heijne, 1997, Protein Engineering 10: 1-6). Of these, 309 have no trans-membrane domain as predicted with TMHMM (A. Krogh, B. Larsson, G. von Heijne, and E. L. L. Sonnhammer, 2000, Journal of Molecular Biology 305: 567-580) and 134 are hypothetical or conserved hypothetical genes. Based on a manual examination of the remaining 175 ORFs, at least 82 were determined to likely encode secreted proteins and enzymes. The sequence ID numbers for each of these genes are listed in Table 3.


Protein secretion, sporulation, and competence pathways. Kunst et al. (Kunst, F., Ogasawara, N, Mozser, I., Albertini, A. M., Alloni, G., Azebedo, V., Bertero, M. G., Bessieres, P., Bolotin, A., and Borchert, S., 1997, Nature 390: 249-256) listed 18 genes that play a major role in the secretion of extracellular enzymes by Bacillus subtilis 168. This list includes several chaperonins, signal peptidases, components of the signal recognition particle and protein translocase complexes. Thus, it is reasonable to expect that the central features of the secretory apparatus are conserved in Bacillus subtilis and Bacillus licheniformis.


From the list of 139 sporulation genes tabulated by Kunst et al. (Kunst, F., Ogasawara, N, Mozser, I., Albertini, A. M., Alloni, G., Azebedo, V., Bertero, M. G., Bessieres, P., Bolotin, A., and Borchert, S. et al., 1997, Nature 390: 249-256), all but six have obvious counterparts in Bacillus licheniformis. These six exceptions (spsABCEFG) comprise an operon involved in synthesis of spore coat polysaccharide in Bacillus subtilis. Additionally, the response regulator gene family (phrACEFGI) appears to have a low level of sequence conservation between Bacillus subtilis and Bacillus licheniformis.


Natural competence (the ability to take up and process exogenous DNA in specific growth conditions) is a feature of few Bacillus licheniformis strains (Gwinn, D. D. and Thorne, C. B., 1964, J. Bacteriol. 87: 519-526). The reasons for variability in competence phenotype have not been explored at the genetic level, but the genome data offered several possible explanations. Although the type strain genome encodes all of the late competence functions ascribed in Bacillus subtilis (e.g., comC, comEFG operons, comK, mecA), it lacks an obvious comS gene, and the comP gene is punctuated by an insertion sequence element, suggesting that the early stages of competence development have been preempted in Bacillus licheniformis ATCC 14580. Whether these early functions can be restored by introducing the corresponding genes from Bacillus subtilis is unknown. In addition to an apparent deficiency in DNA uptake, two Type I restriction-modification systems were discovered that may also contribute diminished transformation efficiencies. These are distinct from the ydiOPS genes of Bacillus subtilis, and could participate in degradation of improperly modified DNA from heterologous hosts used during construction of recombinant expression vectors. Lastly, the synthesis of a glutamyl polypeptide capsule has also been implicated as a potential barrier to transformation of Bacillus licheniformis strains (Thorne, C. B. and Stull, H. B., 1966, J. Bacteriol. 91: 1012-1020). Six genes were predicted (ywtABDEF and ywsC orthologues) that may be involved in the synthesis of this capsular material.


Antibiotics and secondary metabolites. Bacitracin is a cyclic peptide antibiotic that is synthesized non-ribosomally in Bacillus licheniformis (Katz, E. and Demain, A. L., 1977, Bacteriol. Rev. 41: 449-474). While there is variation in the prevalence of bacitracin synthase genes in laboratory strains of this species, one study suggested that up to 50% may harbor the bac operon (Ishihara, H., Takoh, M., Nishibayashi, R., and Sato, A., 2002, Curr. Microbiol. 45: 18-23). The bac operon was determined not to be present in the type strain (ATCC 14580) genome. Seemingly, the only non-ribosomal peptide synthase operon encoded by the Bacillus licheniformis type strain genome is that which is responsible for lichenysin biosynthesis. Lichenysin structurally resembles surfactin from Bacillus subtilis (Grangemard, I., Wallach, J., Maget-Dana, R., and Peypoux, F., 2001, Appl. Biochem. Biotechnol. 90: 199-210), and their respective biosynthetic operons are highly similar. No Bacillus licheniformis counterparts were found for the pps (plipastatin synthase) and polyketide synthase (pks) operons of Bacillus subtilis. Collectively, these two regions represent sizeable portions (80 kb and 38 kb, respectively) of the chromosome in Bacillus subtilis, although they are reportedly dispensable (Westers, H., Dorenbos, R., van Dijl, J. M., Kable, J., Flanagan, T., Devine, K. M., Jude, F., Seror, S. J., Beekman, A. C., Darmon, E., 2003, Mol. Biol. Evol. 20: 2076-2090). Unexpectedly, a gene cluster was found encoding a lantibiotic and associated processing and transport functions. This peptide of 69 amino acids was designated as lichenicidin, and its closest known orthologue is mersacidin from Bacillus sp. strain HIL-Y85/54728 (Altena, K., Guder, A., Cramer, C., and Bierbaum, G., 2000, Appl. Environ. Microbiol. 66: 2565-2571). Lantibiotics are ribosomally synthesized peptides that are modified post-translationally so that the final molecules contain rare thioether amino acids such as lanthionine and/or methyl-lanthionine (Pag, U. and Sahl, H. G., 2002, Curr. Pharm. Des. 8: 815-833). These antimicrobial compounds have attracted much attention in recent years as models for the design of new antibiotics (Hoffmann, A., Pag, U., Wiedemann, I., and Sahl, H. G., 2002, Farmaco. 57: 685-691).


Essential Genes. The gene models were also compared to the list of essential genes in Bacillus subtilis (Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., Asai, K., Ashikaga, S., Aymerch, S., Bessieres, P., 2003, Proc. Natl Acad. Sci. USA 100: 4678-4683). All the essential genes in Bacillus subtilis have orthologues in Bacillus licheniformis, and most are present in a wide range of bacterial taxa (Pedersen, P. B., Bjørnvad, M. E., Rasmussen, M. D., and Petersen, J. N., 2002, Reg. Toxicol. Pharmacol. 36: 155-161).


Example 4
Comparison of Bacillus licheniformis Genome with Other Bacilli

VisualGenome software (Rational Genomics, San Francisco, Calif.) was used for GC-skew analysis and global homology comparisons of the Bacillus licheniformis, Bacillus subtilis, and Bacillus halodurans genomes with pre-computed BLAST results stored in a local database. In pairwise comparisons (E-score threshold of 10−5) 66% (2771/4208) of the predicted Bacillus licheniformis ORFs have orthologs in Bacillus subtilis, and 55% (2321/4208) of the gene models are represented by orthologous sequences in Bacillus halodurans. Using a reciprocal BLASTP analysis 1719 orthologs were found that are common to all three species (E-score threshold of 10−5).


As noted by Lapidus et al. (Lapidus, A., Galleron, N., Andersen, J. T., Jørgensen, P. L. Ehrlich, S. D., and Sorokin, A., 2002, FEMS Microbiol. Lett. 209: 23-30), there are broad regions of colinearity between the genomes of Bacillus licheniformis and Bacillus subtilis. Less conservation of genome organization exists between Bacillus licheniformis and Bacillus halodurans, and inversion of one or more large genomic segments is evident. Clearly this supports previous findings (Xu, D. and Côté, J. C., 2003, Internat. J. Syst. Evol. Microbiol. 53: 695-704) that Bacillus subtilis and Bacillus licheniformis are phylogenetically and evolutionarily closer than either species is to Bacillus halodurans. However, a number of important differences were also observed, both in the numbers and locations of prophages and transposable elements and in a number of biochemical pathways, which distinguish Bacillus licheniformis from Bacillus subtilis, including a region of more than 80 kb that comprises a cluster of polyketide synthase genes that are absent in Bacillus licheniformis.


Example 5
Codon Usage Tables

The evolution of codon bias, the unequal usage of synonomous codons, is thought to be due to natural selection for the use of preferred codons that match the most abundant species of isoaccepting tRNAs, resulting in increased translational efficiency and accuracy. The practical applications for utilizing codon bias information include optimizing expression of heterologous and mutant genes (Jiang and Mannervik, 1999, Protein Expression and Purification 15: 92-98), site-directed mutagenesis to derive variant polypeptides from a given gene (Wong at al., 1995. J. Immunol. 154: 3351-3358; Kaji, H. et al., 1999, J. Biochem. 126: 769-775), design and synthesis of synthetic genes (Libertini and Di Donato, 1992, Protein Engineering 5: 821-825: Feng et al., 2000, Biochem. 39: 15399-15409), and fine-tuning or reducing of translation efficiency of specific genes by introduction of non-preferred codons (Crombie, T. et al., 1992, J. Mol. Biol. 228: 7-12; Carlini and Stephan, 2003, Genetics 163: 239-243).


A codon usage table (Table 4) was generated from SEQ ID NO: 1 with CUSP, a software component of the EMBOSS package (Rice, Longden, and Bleasby, 2000, EMBOSS: The European Molecular Biology Open Software Suite. Trends in Genetics 16: 276-277) on all the predicted protein-coding genes of the Bacillus licheniformis chromosome. CUSP read the coding sequences and calculated the codon frequency table shown in Table 4.


A codon usage table (Table 5) was also generated based on the signal peptides of the 82 extracellular proteins described in Example 3.









TABLE 1







Predicted functions
















Bacillus








subtilis



SEQ



homolog


ID



(Gene


NO.
Description
UniRef Accession No.
Organism
Name)














2
Chromosomal replication initiator
UniRef100_P05648

Bacillus subtilis

DnaA



protein dnaA [Bacillus subtilis]


3
DNA polymerase III, beta chain
UniRef100_P05649

Bacillus subtilis

DnaN



[Bacillus subtilis]


4


5
DNA replication and repair protein recF
UniRef100_P05651

Bacillus subtilis

RecF



[Bacillus subtilis]


6


7
DNA gyrase subunit B [Bacillus subtilis]
UniRef100_P05652

Bacillus subtilis

GyrB


8
DNA gyrase subunit A [Bacillus subtilis]
UniRef100_P05653

Bacillus subtilis

GyrA


9



YaaC


10
Inosine-5′-monophosphate
UniRef100_P21879

Bacillus subtilis

GuaB



dehydrogenase [Bacillus subtilis]


11
D-alanyl-D-alanine carboxypeptidase
UniRef100_P08750

Bacillus subtilis

DacA



precursor [Bacillus subtilis]


12
Pyridoxine biosynthesis protein pdx1
UniRef100_P37527

Bacillus subtilis

YaaD



[Bacillus subtilis]


13
Hypothetical UPF0030 protein yaaE
UniRef100_P37528

Bacillus subtilis

YaaE



[Bacillus subtilis]


14
Seryl-tRNA synthetase [Bacillus
UniRef100_P37464

Bacillus subtilis

SerS




subtilis]



15
Glycerate kinase [Bacillus subtilis]
UniRef100_P42100

Bacillus subtilis

YxaA


16
H+/gluconate symporter [Vibrio
UniRef100_Q7MHW6

Vibrio vulnificus

YuiF




vulnificus]



17
Sugar diacid utilization regulator [Vibrio
UniRef100_Q8DBZ9

Vibrio vulnificus

YsfB




vulnificus]



18
Hypothetical protein [Bacillus
UniRef100_Q6HH43

Bacillus thuringiensis





thuringiensis]



19
Hypothetical protein yaaF [Bacillus
UniRef100_P37529

Bacillus subtilis

Dck




subtilis]



20
Hypothetical protein yaaG [Bacillus
UniRef100_P37530

Bacillus subtilis

Dgk




subtilis]



21
Hypothetical protein yaaH [Bacillus
UniRef100_P37531

Bacillus subtilis

YaaH




subtilis]



22
Hypothetical protein yaaI [Bacillus
UniRef100_P37532

Bacillus subtilis

YaaI




subtilis]



23



YaaJ


24



DnaX


25
Hypothetical UPF0133 protein yaaK
UniRef100_P24281

Bacillus subtilis

YaaK



[Bacillus subtilis]


26
Recombination protein recR [Bacillus
UniRef100_P24277

Bacillus subtilis

RecR




subtilis]



27
Hypothetical protein yaaL [Bacillus
UniRef100_P37533

Bacillus subtilis





subtilis]



28
Sigma-K factor processing regulatory
UniRef100_P24282

Bacillus subtilis




protein BOFA [Bacillus subtilis]


29
CsfB protein [Bacillus subtilis]
UniRef100_P37534

Bacillus subtilis



30
XpaC protein [Bacillus subtilis]
UniRef100_P37467

Bacillus subtilis

XpaC


31
Hypothetical protein yaaN [Bacillus
UniRef100_P37535

Bacillus subtilis

YaaN




subtilis]



32



YaaO


33
Thymidylate kinase [Bacillus subtilis]
UniRef100_P37537

Bacillus subtilis

Tmk


34
Hypothetical protein yaaQ [Bacillus
UniRef100_P37538

Bacillus subtilis

YaaQ




subtilis]



35


36
DNA polymerase III, delta′ subunit
UniRef100_P37540

Bacillus subtilis

HolB



[Bacillus subtilis]


37
Hypothetical protein yaaT [Bacillus
UniRef100_P37541

Bacillus subtilis

YaaT




subtilis]



38
Hypothetical protein yabA [Bacillus
UniRef100_P37542

Bacillus subtilis

YabA




subtilis]



39
Hypothetical protein yabB [Bacillus
UniRef100_P37543

Bacillus subtilis

YabB




subtilis]



40
Hypothetical UPF0213 protein yazA
UniRef100_O31414

Bacillus subtilis




[Bacillus subtilis]


41
Hypothetical UPF0011 protein yabC
UniRef100_P37544

Bacillus subtilis

YabC



[Bacillus subtilis]


42
Transition state regulatory protein abrB
UniRef100_P08874

Bacillus subtilis




[Bacillus subtilis]


43
Methionyl-tRNA synthetase [Bacillus
UniRef100_P37465

Bacillus subtilis

MetS




subtilis]



44
Putative deoxyribonuclease yabD
UniRef100_P37545

Bacillus subtilis

YabD



[Bacillus subtilis]


45



YabE


46
Hypothetical protein yabF [Bacillus
UniRef100_P37547

Bacillus subtilis

RnmV




subtilis]



47
Dimethyladenosine transferase (EC
UniRef100_P37468

Bacillus subtilis

KsgA



2.1.1.—) (S-adenosylmethionine-6-N′,N′-



adenosyl(rRNA) dimethyltransferase)



[Bacillus subtilis]


48
Hypothetical protein yabG [Bacillus
UniRef100_P37548

Bacillus subtilis

YabG




subtilis]



49
Veg protein [Bacillus subtilis]
UniRef100_P37466

Bacillus subtilis



50
SspF protein [Bacillus subtilis]
UniRef100_P37549

Bacillus subtilis



51



IspE


52
Pur operon repressor [Bacillus subtilis]
UniRef100_P37551

Bacillus subtilis

PurR


53
UPF0076 protein yabJ [Bacillus subtilis]
UniRef100_P37552

Bacillus subtilis

YabJ


54
Stage V sporulation protein G [Bacillus
UniRef100_P28015

Bacillus subtilis





subtilis]



55
Bifunctional gcaD protein (TMS protein)
UniRef100_P14192
Includes; UDP-
GcaD



[Includes: UDP-N-acetylglucosamine

N-



pyrophosphorylase (EC 2.7.7.23) (N-

acetylglucosamine



acetylglucosamine-1-phosphate

pyrophosphorylase



uridyltransferase); Glucosamine-1-

(EC 2.7.7.23)



phosphate N-acetyltransferase (EC

(N-



2.3.1.157)] [Bacillus subtilis]

acetylglucosamine-





1-phosphate





uridyltransferase);





Glucosamine-1-





phosphate N-





acetyltransferase





(EC 2.3.1.157)


56
Ribose-phosphate pyrophosphokinase
UniRef100_P14193

Bacillus subtilis

Prs



[Bacillus subtilis]


57
General stress protein ctc [Bacillus
UniRef100_P14194

Bacillus subtilis

Ctc




subtilis]



58
Peptidyl-tRNA hydrolase [Bacillus
UniRef100_P37470

Bacillus subtilis

SpoVC




subtilis]



59
Hypothetical protein yabK [Bacillus
UniRef100_P37553

Bacillus subtilis





subtilis]



60
Transcription-repair coupling factor
UniRef100_P37474

Bacillus subtilis

Mfd



[Bacillus subtilis]


61
Stage V sporulation protein T [Bacillus
UniRef100_P37554

Bacillus subtilis

SpoVT




subtilis]



62
Hypothetical protein yabM [Bacillus
UniRef100_P37555

Bacillus subtilis

YabM




subtilis]



63
Hypothetical protein yabN [Bacillus
UniRef100_P37556

Bacillus subtilis

YabN




subtilis]



64
Hypothetical protein yabO [Bacillus
UniRef100_P37557

Bacillus subtilis





subtilis]



65
Hypothetical protein yabP [Bacillus
UniRef100_P37558

Bacillus subtilis

YabP




subtilis]



66
Hypothetical protein yabQ [Bacillus
UniRef100_P37559

Bacillus subtilis

YabQ




subtilis]



67
Cell division protein divlC [Bacillus
UniRef100_P37471

Bacillus subtilis

DivlC




subtilis]



68
Hypothetical protein yabR [Bacillus
UniRef100_P37560

Bacillus subtilis

YabR




subtilis]



69
Stage II sporulation protein E [Bacillus
UniRef100_P37475

Bacillus subtilis

SpoIIE




subtilis]



70
Hypothetical protein yabS [Bacillus
UniRef100_P37561

Bacillus subtilis

YabS




subtilis]



71
Probable serine/threonine-protein
UniRef100_P37562

Bacillus subtilis

YabT



kinase yabT [Bacillus subtilis]


72
Hypothetical UPF0072 protein yacA
UniRef100_P37563

Bacillus subtilis

YacA



[Bacillus subtilis]


73
Hypoxanthine-guanine
UniRef100_P37472

Bacillus subtilis

HprT



phosphoribosyltransferase [Bacillus




subtilis]



74
Cell division protein ftsH homolog
UniRef100_P37476

Bacillus subtilis

FtsH



[Bacillus subtilis]


75
Putative 32 kDa replication protein
UniRef100_Q9F985

Bacillus

YacB



[Bacillus stearothermophilus]


stearothermophilus



76
33 kDa chaperonin [Bacillus subtilis]
UniRef100_P37565

Bacillus subtilis

YacC


77



YacD


78



CysK


79
Para-aminobenzoate synthase
UniRef100_P28820

Bacillus subtilis

PabB



component I [Bacillus subtilis]


80
Para-aminobenzoate/anthranilate
UniRef100_P28819
Includes: Para-
PabA



synthase glutamine amidotransferase

aminobenzoate



component II [Includes; Para-

synthase



aminobenzoate synthase glutamine

glutamine



amidotransferase component II (EC

amidotransferase



6.3.5.8) (ADC synthase); Anthranilate

component II



synthase component II (EC 4.1.3.27)]

(EC 6.3.5.8)



[Bacillus subtil

(ADC synthase);





Anthranilate





synthase





component II





(EC 4.1.3.27)


81
Aminodeoxychorismate lyase [Bacillus
UniRef100_P28821

Bacillus subtilis

PabC




subtilis]



82
Dihydropteroate synthase [Bacillus
UniRef100_P28822

Bacillus subtilis

SuI




subtilis]



83
Dihydroneopterin aldolase [Bacillus
UniRef100_P28823

Bacillus subtilis

FolB




subtilis]



84
2-amino-4-hydroxy-6-
UniRef100_P29252

Bacillus subtilis

FolK



hydroxymethyldihydropteridine



pyrophosphokinase [Bacillus subtilis]


85
YazB protein [Bacillus subtilis]
UniRef100_O31417

Bacillus subtilis



86
Probable tRNA-dihydrouridine synthase
UniRef100_P37567

Bacillus subtilis

YacF



1 [Bacillus subtilis]


87
Lysyl-tRNA synthetase [Bacillus
UniRef100_P37477

Bacillus subtilis

LysS




subtilis]



88
Transcriptional regulator ctsR [Bacillus
UniRef100_P37568

Bacillus subtilis

CtsR




subtilis]



89
Hypothetical protein yacH [Bacillus
UniRef100_P37569

Bacillus subtilis

McsA




subtilis]



90
Hypothetical ATP:guanido
UniRef100_P37570

Bacillus subtilis

McsB



phosphotransferase yacI [Bacillus




subtilis]



91
Negative regulator of genetic
UniRef100_P37571

Bacillus subtilis

ClpC



competence clpC/mecB [Bacillus




subtilis]



92



RadA


93
Hypothetical protein yacK [Bacillus
UniRef100_P37573

Bacillus subtilis

YacK




subtilis]



94
Hypothetical protein yacL [Bacillus
UniRef100_Q06754

Bacillus subtilis

YacL




subtilis]



95
2-C-methyl-D-erythritol 4-phosphate
UniRef100_Q06755

Bacillus subtilis

YacM



cytidylyltransferase [Bacillus subtilis]


96
2-C-methyl-D-erythritol 2,4-
UniRef100_Q06756

Bacillus subtilis

YacN



cyclodiphosphate synthase [Bacillus




subtilis]



97
Glutamyl-tRNA synthetase [Bacillus
UniRef100_P22250

Bacillus subtilis

GltX




subtilis]



98
Serine acetyltransferase [Bacillus
UniRef100_Q06750

Bacillus subtilis

CysE




subtilis]



99
Cysteinyl-tRNA synthetase [Bacillus
UniRef100_Q06752

Bacillus subtilis

CysS




subtilis]



100
YazC protein [Bacillus subtilis]
UniRef100_O31418

Bacillus subtilis

YazC


101
Hypothetical tRNA/rRNA
UniRef100_Q06753

Bacillus subtilis

YacO



methyltransferase yacO [Bacillus




subtilis]



102
Hypothetical protein yacP [Bacillus
UniRef100_P37574

Bacillus subtilis

YacP




subtilis]



103
RNA polymerase sigma-H factor
UniRef100_P17869

Bacillus subtilis

SigH



[Bacillus subtilis]


104
Preprotein translocase secE subunit
UniRef100_Q06799

Bacillus subtilis




[Bacillus subtilis]


105



NusG


106
50S ribosomal protein L11 [Bacillus
UniRef100_Q06796

Bacillus subtilis

RplK




subtilis]



107
50S ribosomal protein L1 [Bacillus
UniRef100_Q06797

Bacillus subtilis

RplA




subtilis]



108
50S ribosomal protein L10 [Bacillus
UniRef100_P42923

Bacillus subtilis

RplJ




subtilis]



109
50S ribosomal protein L7/L12 [Bacillus
UniRef100_P02394

Bacillus subtilis

RplL




subtilis]



110
Hypothetical protein ybxB [Bacillus
UniRef100_P37872

Bacillus subtilis

YbxB




subtilis]



111
DNA-directed RNA polymerase beta
UniRef100_P37870

Bacillus subtilis

RpoB



chain [Bacillus subtilis]


112
DNA-directed RNA polymerase beta′
UniRef100_P37871

Bacillus subtilis

RpoC



chain [Bacillus subtilis]


113
Putative ribosomal protein L7Ae-like
UniRef100_P46350

Bacillus subtilis




[Bacillus subtilis]


114
30S ribosomal protein S12 [Bacillus
UniRef100_P21472

Bacillus subtilis

RpsL




subtilis]



115
30S ribosomal protein S7 [Bacillus
UniRef100_P21469

Bacillus subtilis

RpsG




subtilis]



116
Elongation factor G [Bacillus subtilis]
UniRef100_P80868

Bacillus subtilis

FusA


117
Elongation factor Tu [Bacillus subtilis]
UniRef100_P33166

Bacillus subtilis

TufA


118
30S ribosomal protein S10 [Bacillus
UniRef100_Q9Z9L5

Bacillus

RpsJ




halodurans]



halodurans



119
50S ribosomal protein L3 [Bacillus
UniRef100_P42920

Bacillus subtilis

RplC




subtilis]



120
50S ribosomal protein L4 [Bacillus
UniRef100_P42921

Bacillus subtilis

RplD




subtilis]



121
50S ribosomal protein L23 [Bacillus
UniRef100_P42924

Bacillus subtilis





subtilis]



122
50S ribosomal protein L2 [Bacillus
UniRef100_P42919

Bacillus subtilis

RplB




subtilis]



123
30S ribosomal protein S19 [Bacillus
UniRef100_P21476

Bacillus subtilis





subtilis]



124
50S ribosomal protein L22 [Bacillus
UniRef100_P42060

Bacillus subtilis

RplV




subtilis]



125
30S ribosomal protein S3 [Bacillus
UniRef100_P21465

Bacillus subtilis

RpsC




subtilis]



126
50S ribosomal protein L16 [Bacillus
UniRef100_P14577

Bacillus subtilis

RplP




subtilis]



127
50S ribosomal protein L29 [Bacillus
UniRef100_P12873

Bacillus subtilis





subtilis]



128
30S ribosomal protein S17 [Bacillus
UniRef100_P12874

Bacillus subtilis





subtilis]



129


130
50S ribosomal protein L24 [Bacillus
UniRef100_P12876

Bacillus subtilis

RplX




subtilis]



131
50S ribosomal protein L5 [Bacillus
UniRef100_P12877

Bacillus subtilis

RplE




subtilis]



132
Ribosomal protein S14 [Bacillus cereus
UniRef100_Q63H77

Bacillus cereus ZK




ZK]


133
30S ribosomal protein S8 [Bacillus
UniRef100_P12879

Bacillus subtilis

RpsH




subtilis]



134
50S ribosomal protein L6 [Bacillus
UniRef100_P46898

Bacillus subtilis

RplF




subtilis]



135
50S ribosomal protein L18 [Bacillus
UniRef100_P46899

Bacillus subtilis

RplR




subtilis]



136
30S ribosomal protein S5 [Bacillus
UniRef100_P21467

Bacillus subtilis

RpsE




subtilis]



137
50S ribosomal protein L30 [Bacillus
UniRef100_P19947

Bacillus subtilis





subtilis]



138
50S ribosomal protein L15 [Bacillus
UniRef100_P19946

Bacillus subtilis

RplO




subtilis]



139



SecY


140
Adenylate kinase [Bacillus subtilis]
UniRef100_P16304

Bacillus subtilis

Adk


141
Methionine aminopeptidase [Bacillus
UniRef100_P19994

Bacillus subtilis

Map




subtilis]



142
C-125 initiation factor IF-I, RNA
UniRef100_O50629

Bacillus halodurans




polymerase alpha subunit and



ribosomal proteins, partial and



complete cds [Bacillus halodurans]


143
Translation initiation factor IF-1
UniRef100_P20458

Bacillus subtilis




[Bacillus subtilis]


144


145



RpsM


146
30S ribosomal protein S11 [Bacillus
UniRef100_P04969

Bacillus subtilis

RpsK




subtilis]



147
DNA-directed RNA polymerase alpha
UniRef100_P20429

Bacillus subtilis

RpoA



chain [Bacillus subtilis]


148
50S ribosomal protein L17 [Bacillus
UniRef100_P20277

Bacillus subtilis

RplQ




subtilis]



149
Hypothetical ABC transporter ATP-
UniRef100_P40735

Bacillus subtilis

YbxA



binding protein ybxA [Bacillus subtilis]


150
Hypothetical protein orf5 [Bacillus
UniRef100_P70970

Bacillus subtilis

YbaE




subtilis]



151
YbaF protein [Bacillus subtilis]
UniRef100_P70972

Bacillus subtilis

YbaF


152
tRNA pseudouridine synthase A
UniRef100_P70973

Bacillus subtilis

TruA



[Bacillus subtilis]


153
50S ribosomal protein L13 [Bacillus
UniRef100_P70974

Bacillus subtilis

RplM




subtilis]



154
30S ribosomal protein S9 [Bacillus
UniRef100_P21470

Bacillus subtilis

RpsI




subtilis]



155
Hypothetical protein [Bacillus cereus]
UniRef100_Q737T6

Bacillus cereus

YizA


156
Hypothetical protein ybaK [Bacillus
UniRef100_P50862

Bacillus subtilis

YbaK




subtilis]



157
Germination-specific N-
UniRef100_P50864

Bacillus subtilis

CwlD



acetylmuramoyl-L-alanine amidase



[Bacillus subtilis]


158



YbaL


159
Spore germination protein gerD
UniRef100_P16450

Bacillus subtilis

GerD



precursor [Bacillus subtilis]


160
KinB signaling pathway activation
UniRef100_P16449

Bacillus subtilis

KbaA



protein [Bacillus subtilis]


161
Hypothetical protein ybaN precursor
UniRef100_P50865

Bacillus subtilis

YbaN



[Bacillus subtilis]


162
Penicillin-binding protein [Bacillus
UniRef100_O31773

Bacillus subtilis

PbpX




subtilis]



163


164
Hypothetical protein ybaS [Bacillus
UniRef100_P55190

Bacillus subtilis

YbaS




subtilis]



165
Hypothetical protein yxaJ [Bacillus
UniRef100_P42109

Bacillus subtilis

YxaJ




subtilis]



166
Phenazine biosynthetic protein
UniRef100_Q9HHG6

Halobacterium

YfhB



[Halobacterium sp.]

sp.


167
Hypothetical protein ybbC precursor
UniRef100_P40407

Bacillus subtilis

YbbC



[Bacillus subtilis]


168
Hypothetical lipoprotein ybbD precursor
UniRef100_P40406

Bacillus subtilis

YbbD



[Bacillus subtilis]


169
Hypothetical UPF0214 protein ybbE
UniRef100_O05213

Bacillus subtilis

YbbE



precursor [Bacillus subtilis]


170
YbbF protein [Bacillus subtilis]
UniRef100_Q797S1

Bacillus subtilis

YbbF


171
Putative HTH-type transcriptional
UniRef100_Q45581

Bacillus subtilis

YbbH



regulator ybbH [Bacillus subtilis]


172
Hypothetical protein ybbI [Bacillus
UniRef100_Q45582

Bacillus subtilis

YbbI




subtilis]



173
Hypothetical protein ybbK [Bacillus
UniRef100_Q45584

Bacillus subtilis

YbbK




subtilis]



174
Arginase [Bacillus caldovelox]
UniRef100_P53608

Bacillus

RocF






caldovelox



175
RNA polymerase sigma factor sigW
UniRef100_Q45585

Bacillus subtilis

SigW



[Bacillus subtilis]


176
YbbM protein [Bacillus subtilis]
UniRef100_Q45588

Bacillus subtilis

YbbM


177
YbbP protein [Bacillus subtilis]
UniRef100_Q45589

Bacillus subtilis

YbbP


178
YbbR protein [Bacillus subtilis]
UniRef100_O34659

Bacillus subtilis

YbbR


179
YbbT protein [Bacillus subtilis]
UniRef100_O34824

Bacillus subtilis

YbbT


180
Glucosamine--fructose-6-phosphate
UniRef100_P39754
isomerizing
GlmS



aminotransferase [isomerizing] [Bacillus




subtilis]



181


182
Hypothetical protein [Bacillus
UniRef100_Q6HH47

Bacillus thuringiensis





thuringiensis]



183
UPI00003CBF92 UniRef100 entry
UniRef100_UPI00003CBF92

YtrB


184
Transcriptional regulator [Bacillus
UniRef100_Q9KF35

Bacillus

YtrA




halodurans]



halodurans



185


186
YbcL protein [Bacillus subtilis]
UniRef100_O34663

Bacillus subtilis

YbcL


187
BH0186 protein [Bacillus halodurans]
UniRef100_Q9KGB8

Bacillus halodurans



188
ABC transporter [Bacillus halodurans]
UniRef100_Q9KEY6

Bacillus

YvcC






halodurans



189
Hypothetical protein ywbO [Bacillus
UniRef100_P39598

Bacillus subtilis

YwbO




subtilis]



190


191
BH0695 protein [Bacillus halodurans]
UniRef100_Q9KF04

Bacillus halodurans



192
Aminoglycoside 6-adenylyltranserase
UniRef100_Q6V4U6

Enterococcus

AadK



[Enterococcus faecium]


faecium



193
Hypothetical conserved protein
UniRef100_Q8ERX2

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


194
YfnB [Bacillus subtilis]
UniRef100_O06480

Bacillus subtilis

YfnB


195
Hypothetical transport protein ybxG
UniRef100_P54425

Bacillus subtilis

YbxG



[Bacillus subtilis]


196
Mg(2+)/citrate complex secondary
UniRef100_P55069

Bacillus subtilis

CitM



transporter [Bacillus subtilis]


197
YflP protein [Bacillus subtilis]
UniRef100_O34439

Bacillus subtilis

YflP


198



CitT


199
Sensor protein citS [Bacillus subtilis]
UniRef100_O34427

Bacillus subtilis

CitS


200
Transcriptional regulator
UniRef100_Q8RB37

Thermoanaerobacter

YwfK



[Thermoanaerobacter tengcongensis]


tengcongensis



201
Complete genome; segment 8/17
UniRef100_Q7N4W7

Photorhabdus

YwfE



[Photorhabdus luminescens]


luminescens



202
Multidrug resistance protein B [Bacillus
UniRef100_Q81AF0

Bacillus cereus

YqjV




cereus]



203
Sigma-G-dependent sporulation
UniRef100_P54379

Bacillus subtilis




specific SASP protein [Bacillus subtilis]


204
Hypothetical protein ybxH [Bacillus
UniRef100_P54426

Bacillus subtilis





subtilis]



205
YbyB protein [Bacillus subtilis]
UniRef100_O31441

Bacillus subtilis



206
Hypothetical protein yyaL [Bacillus
UniRef100_P37512

Bacillus subtilis

YyaL




subtilis]



207
Hypothetical protein yyaO [Bacillus
UniRef100_P37509

Bacillus subtilis





subtilis]



208
Threonyl-tRNA synthetase 2 [Bacillus
UniRef100_P18256

Bacillus subtilis

ThrZ




subtilis]



209


210



YttB


211
Hypothetical protein [Acinetobacter sp.]
UniRef100_Q6FDN0

Acinetobacter sp.



212
Hypothetical UPF0053 protein yrkA
UniRef100_P54428

Bacillus subtilis

YrkA



[Bacillus subtilis]


213



YdeG


214



YbaJ


215


216



YqeW


217
YubF protein [Bacillus subtilis]
UniRef100_O32082

Bacillus subtilis



218



YbfF


219
Hypothetical protein [Bacteroides
UniRef100_Q64UG6

Bacteroides fragilis





fragilis]



220
Hypothetical transport protein ybhF
UniRef100_O31448

Bacillus subtilis

YbfH



[Bacillus subtilis]


221
YbfI protein [Bacillus subtilis]
UniRef100_O31449

Bacillus subtilis

YbfI


222


223
UPI00003CBC98 UniRef100 entry
UniRef100_UPI00003CBC98


224


225
Oxidoreductase [Lactococcus lactis]
UniRef100_Q9CFC5

Lactococcus lactis



226
Methyl-accepting chemotaxis protein
UniRef100_Q8EST3

Oceanobacillus

YvaQ



[Oceanobacillus iheyensis]


iheyensis



227
D-xylose-binding protein
UniRef100_O68456

Thermoanaerobacter

RbsB



[Thermoanaerobacter ethanolicus]


ethanolicus



228
Hypothetical protein OB0544
UniRef100_Q8ESS4

Oceanobacillus

YomI



[Oceanobacillus iheyensis]


iheyensis



229
Hypothetical protein [Bacillus cereus]
UniRef100_Q81IR4

Bacillus cereus



230
Streptogramin B lactonase
UniRef100_O87275

Staphylococcus cohnii




[Staphylococcus cohnii]


231
Hypothetical protein ycbP [Bacillus
UniRef100_P42248

Bacillus subtilis

YcbP




subtilis]



232
YbgF protein [Bacillus subtilis]
UniRef100_O31462

Bacillus subtilis

YbgF


233
YbgG protein [Bacillus subtilis]
UniRef100_O31463

Bacillus subtilis

YbgG


234


235
PTS system, n-acetylglucosamine-
UniRef100_Q9KF24

Bacillus

NagP



specific enzyme II, ABC component


halodurans




[Bacillus halodurans]


236
UPI00003CBDBC UniRef100 entry
UniRef100_UPI00003CBDBC

Mta


237
Hypothetical protein [Bacillus
UniRef100_Q6HIW0

Bacillus

YdfS




thuringiensis]



thuringiensis



238
Carbonic anhydrase [Methanosarcina
UniRef100_Q8PSJ1

Methanosarcina

YtoA




mazei]



mazei



239
Hypothetical protein yjgA [Bacillus
UniRef100_Q6HHU2

Bacillus

YjgA




thuringiensis]



thuringiensis



240
Amino acid carrier protein [Bacillus
UniRef100_Q6HGU4

Bacillus

YbgH




thuringiensis]



thuringiensis



241
Probable glutaminase ybgJ [Bacillus
UniRef100_O31465

Bacillus subtilis

YbgJ




subtilis]



242
Two-component sensor kinase ycbA
UniRef100_Q81BN8

Bacillus cereus

YcbA



[Bacillus cereus]


243
Hypothetical sensory transduction
UniRef100_P40759

Bacillus subtilis

YcbB



protein ycbB [Bacillus subtilis]


244



YoaA


245



YbxI


246


247
Beta-lactamase precursor (EC 3.5.2.6)
UniRef100_P00808
Contains: Large
PenP



(Penicillinase) [Contains: Large

exopenicillinase;



exopenicillinase; Small

Small



exopenicillinase] [Bacillus licheniformis]

exopenicillinase


248
Alkaline phosphatase D precursor
UniRef100_P42251

Bacillus subtilis

PhoD



[Bacillus subtilis]


249


250
Hypothetical protein ycbT [Bacillus
UniRef100_P42252

Bacillus subtilis

TatCD




subtilis]



251



YcbC


252
Probable aldehyde dehydrogenase
UniRef100_P42236

Bacillus subtilis

YcbD



ycbD [Bacillus subtilis]


253
Probable glucarate transporter [Bacillus
UniRef100_P42237

Bacillus subtilis

YcbE




subtilis]



254
Probable glucarate dehydratase
UniRef100_P42238

Bacillus subtilis

YcbF



[Bacillus subtilis]


255
Hypothetical transcriptional regulator
UniRef100_P42239

Bacillus subtilis

YcbG



ycbG [Bacillus subtilis]


256
Probable D-galactarate dehydratase
UniRef100_P42240

Bacillus subtilis

YcbH



[Bacillus subtilis]


257
Hypothetical sensory transduction
UniRef100_P42244

Bacillus subtilis

YcbL



protein ycbL [Bacillus subtilis]


258
Sensor histidine kinase [Bacillus cereus
UniRef100_Q633Q6

Bacillus cereus ZK




ZK]


259
Hypothetical ABC transporter ATP-
UniRef100_P42246

Bacillus subtilis

YcbN



binding protein ycbN [Bacillus subtilis]


260
Hypothetical protein ycbO [Bacillus
UniRef100_P42247

Bacillus subtilis

YcbO




subtilis]



261



YcbO


262
YfnK [Bacillus subtilis]
UniRef100_O06490

Bacillus subtilis

YetN


263
Hypothetical protein ybdO [Bacillus
UniRef100_O31437

Bacillus subtilis

YbdO




subtilis]



264
Hypothetical protein ycbJ [Bacillus
UniRef100_P42242

Bacillus subtilis

YcbJ




subtilis]



265
Hypothetical protein ywhA [Bacillus
UniRef100_P70993

Bacillus subtilis

YwhA




subtilis]



266



YdaB


267
AII0778 protein [Anabaena sp.]
UniRef100_Q8YYR8

Anabaena sp.



268
BH1298 protein [Bacillus halodurans]
UniRef100_Q9KDB5

Bacillus

YbdN






halodurans



269
BH1299 protein [Bacillus halodurans]
UniRef100_Q9KDB4

Bacillus halodurans



270
Transcriptional activator tipA, putative
UniRef100_Q81XN3

Bacillus

Mta



[Bacillus anthracis]


anthracis



271


272
Transcriptional regulator, PadR family
UniRef100_Q81BZ0

Bacillus cereus




[Bacillus cereus]


273
Hypothetical protein [Bacillus cereus
UniRef100_Q639U7

Bacillus cereus ZK




ZK]


274
Tryptophan RNA-binding attenuator
UniRef100_O31466

Bacillus subtilis




protein-inhibitory protein [Bacillus




subtilis]



275
Hypothetical transport protein ycbK
UniRef100_P42243

Bacillus subtilis

YcbK



[Bacillus subtilis]


276



YczC


277
Hypothetical protein yccF [Bacillus
UniRef100_O34478

Bacillus subtilis

YccF




subtilis]



278
SpaF [Bacillus subtilis]
UniRef100_Q45404

Bacillus subtilis

YhcH


279
SpaE [Bacillus subtilis]
UniRef100_O52853

Bacillus subtilis



280
Putative SpaG [Bacillus subtilis]
UniRef100_Q93GG9

Bacillus subtilis



281
Subtilin biosynthesis regulatory protein
UniRef100_P33112

Bacillus subtilis

YycF



spaR [Bacillus subtilis]


282
Putative histidine kinase [Bacillus
UniRef100_Q93GG7

Bacillus subtilis

ResE




subtilis]



283
UPI00003CA401 UniRef100 entry
UniRef100_UPI00003CA401

YtlI


284
YusQ protein [Bacillus subtilis]
UniRef100_O32183

Bacillus subtilis

YusQ


285
Complete genome; segment 6/17
UniRef100_Q7N6K9

Photorhabdus

YusR



[Photorhabdus luminescens]


luminescens



286
Methyltransferase [Bacillus
UniRef100_Q6HK82

Bacillus thuringiensis





thuringiensis]



287
Hypothetical lipoprotein ycdA precursor
UniRef100_O34538

Bacillus subtilis

YcdA



[Bacillus subtilis]


288
UPI00003CB481 UniRef100 entry
UniRef100_UPI00003CB481

YcgA


289
Hypothetical conserved protein
UniRef100_Q8ESQ2

Oceanobacillus

YtnL



[Oceanobacillus iheyensis]


iheyensis



290
Hypothetical conserved protein
UniRef100_Q8ET83

Oceanobacillus

YvbK



[Oceanobacillus iheyensis]


iheyensis



291



GatA


292
Oligoendopeptidase F, putative
UniRef100_Q81JJ8

Bacillus

YjbG



[Bacillus anthracis]


anthracis



293


294
UPI00003CC42D UniRef100 entry
UniRef100_UPI00003CC42D

PbpX


295
Hypothetical protein ysfD [Bacillus
UniRef100_P94534

Bacillus subtilis

YsfD




subtilis]



296
Hypothetical protein ysfC [Bacillus
UniRef100_P94535

Bacillus subtilis

YsfC




subtilis]



297
PTS system, cellobiose-specific
UniRef100_Q9KEE3

Bacillus halodurans




enzyme II, B component [Bacillus




halodurans]



298
PTS system, cellobiose-specific
UniRef100_Q9KEE2

Bacillus

LicA



enzyme II, A component [Bacillus


halodurans





halodurans]



299
PTS system, cellobiose-specific
UniRef100_Q8EP43

Oceanobacillus

YwbA



enzyme II, C component


iheyensis




[Oceanobacillus iheyensis]


300
6-phospho-beta-glucosidase [Bacillus
UniRef100_Q9KEE0

Bacillus

LicH




halodurans]



halodurans



301
Transcriptional regulator [Bacillus
UniRef100_Q9KED8

Bacillus

YbgA




halodurans]



halodurans



302


303



YvbX


304
Chitinase precursor [Streptomyces
UniRef100_Q9L3E8

Streptomyces olivaceoviridis





olivaceoviridis]



305
Extracellular metalloprotease precursor
UniRef100_P39790

Bacillus subtilis

Mpr



[Bacillus subtilis]


306
Glucose 1-dehydrogenase II [Bacillus
UniRef100_P80869

Bacillus subtilis

YcdF




subtilis]



307
Hypothetical protein OB0244
UniRef100_Q8ETL6

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


308
Hypothetical protein ycdC [Bacillus
UniRef100_O34772

Bacillus subtilis

YcdC




subtilis]



309


310
Cell wall hydrolase cwlJ [Bacillus
UniRef100_P42249

Bacillus subtilis

CwlJ




subtilis]



311
Hypothetical protein yceB [Bacillus
UniRef100_O34504

Bacillus subtilis

YceB




subtilis]



312
Hypothetical protein yvcE [Bacillus
UniRef100_P40767

Bacillus subtilis

YvcE




subtilis]



313
ABC transporter, permease [Bacillus
UniRef100_Q637N2

Bacillus cereus

YfiM




cereus ZK]


ZK


314
ABC transporter, ATP-binding protein
UniRef100_Q733P8

Bacillus cereus

YfiL



[Bacillus cereus]


315
UPI00003CC482 UniRef100 entry
UniRef100_UPI00003CC482

YsiA


316
Hypothetical protein OB3113
UniRef100_Q8ELV2

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


317
BH3953 protein [Bacillus halodurans]
UniRef100_Q9K5Y3

Bacillus halodurans



318
BH3951 protein [Bacillus halodurans]
UniRef100_Q9K5Y4

Bacillus

PadR






halodurans



319
Stress response protein SCP2 [Bacillus
UniRef100_P81100

Bacillus subtilis

YceC




subtilis]



320
General stress protein 16U [Bacillus
UniRef100_P80875

Bacillus subtilis

YceD




subtilis]



321
Hypothetical protein yceE [Bacillus
UniRef100_O34384

Bacillus subtilis

YceE




subtilis]



322
Hypothetical protein yceF [Bacillus
UniRef100_O34447

Bacillus subtilis

YceF




subtilis]



323
Hypothetical protein [Bacillus cereus]
UniRef100_Q72YD0

Bacillus cereus



324
YceG [Bacillus subtilis]
UniRef100_O34809

Bacillus subtilis

YceG


325
Hypothetical protein yceH [Bacillus
UniRef100_O34833

Bacillus subtilis

YceH




subtilis]



326
UPI00003CB694 UniRef100 entry
UniRef100_UPI00003CB694

CcdA


327
Hypothetical conserved protein
UniRef100_Q8ELB4

Oceanobacillus

YdiL



[Oceanobacillus iheyensis]


iheyensis



328



Mta


329
Nitrate transporter [Bacillus subtilis]
UniRef100_P42432

Bacillus subtilis

NasA


330
L-lactate dehydrogenase [Bacillus
UniRef100_P13714

Bacillus subtilis

Ldh




subtilis]



331
L-lactate permease [Bacillus subtilis]
UniRef100_P55910

Bacillus subtilis

LctP


332
YcgF protein [Bacillus subtilis]
UniRef100_P94381

Bacillus subtilis

YcgF


333
Homologue of aromatic amino acids
UniRef100_P94383

Bacillus subtilis

YcgH



transport protein of E. coli [Bacillus




subtilis]



334
NH(3)-dependent NAD(+) synthetase
UniRef100_P08164

Bacillus subtilis

NadE



[Bacillus subtilis]


335
Shikimate kinase [Bacillus subtilis]
UniRef100_P37944

Bacillus subtilis

AroK


336
YcgL protein [Bacillus subtilis]
UniRef100_P94389

Bacillus subtilis

YcgL


337
Proline dehydrogenase [Bacillus
UniRef100_Q8RL79

Bacillus subtilis

YcgM




subtilis]



338
1-pyrroline-5-carboxylate
UniRef100_P94391

Bacillus subtilis

YcgN



dehydrogenase 2 [Bacillus subtilis]


339
Homologue of proline permease of E. coli
UniRef100_P94392

Bacillus subtilis

YcgO



[Bacillus subtilis]


340
Hypothetical protein ycgP [Bacillus
UniRef100_P94393

Bacillus subtilis

YcgP




subtilis]



341
YcgQ protein [Bacillus subtilis]
UniRef100_P94394

Bacillus subtilis

YcgQ


342



YcgR


343
Cephalosporin-C deacetylase [Bacillus
UniRef100_Q59233

Bacillus subtilis

Cah




subtilis]



344
Transcriptional regulator [Bacillus
UniRef100_Q9KF41

Bacillus

YdgG




halodurans]



halodurans



345


346


347
Probable amino-acid ABC transporter
UniRef100_P42399

Bacillus subtilis

YckA



permease protein yckA [Bacillus




subtilis]



348
Probable ABC transporter extracellular
UniRef100_P42400

Bacillus subtilis

YckB



binding protein yckB precursor [Bacillus




subtilis]



349
NAD(P)H dehydrogenase (Quinone);
UniRef100_Q63FG6

Bacillus cereus

YwrO



possible modulator of drug activity B

ZK



[Bacillus cereus ZK]


350
BH0315 protein [Bacillus halodurans]
UniRef100_Q9KG01

Bacillus halodurans



351
Probable beta-glucosidase [Bacillus
UniRef100_P42403

Bacillus subtilis

YckE




subtilis]



352
Nin [Bacillus amyloliquefaciens]
UniRef100_Q70KK3

Bacillus

Nin






amyloliquefaciens



353
DNA-entry nuclease [Bacillus subtilis]
UniRef100_P12667

Bacillus subtilis

NucA


354



YbdM


355
Methyl-accepting chemotaxis protein
UniRef100_P39209

Bacillus subtilis

TlpC



tlpC [Bacillus subtilis]


356
Hypothetical protein yqcG [Bacillus
UniRef100_P45942

Bacillus subtilis

YqcG




subtilis]



357
Hydantoin utilization protein A
UniRef100_Q7WCS3

Bordetella bronchiseptica




[Bordetella bronchiseptica]


358
Hydantoin utilization protein B
UniRef100_Q987J6

Rhizobium loti




[Rhizobium loti]


359
UPI000027D233 UniRef100 entry
UniRef100_UPI000027D233

YvfK


360
358aa long hypothetical transporter
UniRef100_Q9YB65

Aeropyrum

MsmX



ATP-binding protein [Aeropyrum pernix]


pernix



361
AttA2-like ABC transporter, permease
UniRef100_Q92ZH0

Rhizobium

YesP



protein [Rhizobium meliloti]


meliloti



362



YurM


363



SrfAA


364



SrfAB


365



SrfAC


366



SrfAD


367



YcxC


368



YcxD


369
4′-phosphopantetheinyl transferase sfp
UniRef100_P39135

Bacillus subtilis

Sfp



[Bacillus subtilis]


370
Predicted esterase of alpha/beta
UniRef100_Q97HP7

Clostridium

YbbA



hydrolase superfamily, YBBA B. subtilis


acetobutylicum




ortholog [Clostridium acetobutylicum]


371
Transcriptional regulator [Clostridium
UniRef100_Q97LX8

Clostridium

YdeE




acetobutylicum]



acetobutylicum



372
YczE [Bacillus subtilis]
UniRef100_Q9F4F8

Bacillus subtilis

YczE


373
Hypothetical protein [Symbiobacterium
UniRef100_Q67MZ7

Symbiobacterium





thermophilum]



thermophilum



374



YfiL


375


376
Hypothetical protein [Symbiobacterium
UniRef100_Q67MZ9

Symbiobacterium





thermophilum]



thermophilum



377


378


379
Methyl-accepting chemotaxis protein
UniRef100_Q6HJV7

Bacillus

TlpB



[Bacillus thuringiensis]


thuringiensis



380
YckI [Bacillus subtilis]
UniRef100_Q9F4F9

Bacillus subtilis

YckI


381
YckJ [Bacillus subtilis]
UniRef100_Q9F4G0

Bacillus subtilis

YckJ


382



YckK


383



RocR


384
Ornithine aminotransferase [Bacillus
UniRef100_P38021

Bacillus subtilis

RocD




subtilis]



385
Amino-acid permease rocE [Bacillus
UniRef100_P39137

Bacillus subtilis

RocE




subtilis]



386
Arginase [Bacillus subtilis]
UniRef100_P39138

Bacillus subtilis

RocF


387
Homologue of als operon regulatory
UniRef100_P94403

Bacillus subtilis

YclA



protein AlsR of B. subtilis [Bacillus




subtilis]



388
Probable aromatic acid decarboxylase
UniRef100_P94404

Bacillus subtilis

YclB



[Bacillus subtilis]


389
Hypothetical protein yclC [Bacillus
UniRef100_P94405

Bacillus subtilis

YclC




subtilis]



390
YclD protein [Bacillus subtilis]
UniRef100_P94406

Bacillus subtilis

YclD


391


392


393


394
Hypothetical protein [Bacillus
UniRef100_Q6HMQ9

Bacillus thuringiensis





thuringiensis]



395
Hypothetical protein OB2810
UniRef100_Q8EMN2

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


396


397



YxiD


398


399



YxiB


400
Sugar ABC transporter [Bacillus
UniRef100_Q9K7B8

Bacillus

RbsB




halodurans]



halodurans



401
Two-component sensor histidine kinase
UniRef100_Q9K7B9

Bacillus

YesM



[Bacillus halodurans]


halodurans



402



YesN


403
Multiple sugar transport system
UniRef100_Q9K7C2

Bacillus

RbsB



[Bacillus halodurans]


halodurans



404
L-arabinose transport ATP-binding
UniRef100_Q9S472

Bacillus

RbsA



protein araG [Bacillus


stearothermophilus





stearothermophilus]



405
L-arabinose membrane permease
UniRef100_Q9S471

Bacillus

RbsC



[Bacillus stearothermophilus]


stearothermophilus



406
Transporter [Bacillus halodurans]
UniRef100_Q9K9F3

Bacillus

YbfB






halodurans



407
YbfA protein [Bacillus subtilis]
UniRef100_O31443

Bacillus subtilis

YbfA


408
Beta-D-galactosidase [Bacillus
UniRef100_Q45093

Bacillus circulans

LacA




circulans]



409



Phy


410
Hypothetical transporter yclF [Bacillus
UniRef100_P94408

Bacillus subtilis

YclF




subtilis]



411
YclG protein [Bacillus subtilis]
UniRef100_P94409

Bacillus subtilis

YclG


412


413



GerKA


414
Spore germination protein KC precursor
UniRef100_P49941

Bacillus subtilis

GerKC



[Bacillus subtilis]


415
Spore germination protein KB [Bacillus
UniRef100_P49940

Bacillus subtilis

GerKB




subtilis]



416



Mta


417
Hypothetical protein yclH [Bacillus
UniRef100_P94411

Bacillus subtilis

YclH




subtilis]



418
Hypothetical protein yclI [Bacillus
UniRef100_P94412

Bacillus subtilis

YclI




subtilis]



419
Hypothetical sensory transduction
UniRef100_P94413

Bacillus subtilis

YclJ



protein yclJ [Bacillus subtilis]


420
Hypothetical sensor-like histidine
UniRef100_P94414

Bacillus subtilis

YclK



kinase yclK [Bacillus subtilis]


421
Methyl-accepting chemotaxis protein
UniRef100_Q9K632

Bacillus

TlpA



[Bacillus halodurans]


halodurans



422
Probable aspartokinase [Bacillus
UniRef100_P94417

Bacillus subtilis

YclM




subtilis]



423
Homologue of ferric anguibactin
UniRef100_P94418

Bacillus subtilis

YclN



transport system permerase protein



FatD of V. anguillarum [Bacillus subtilis]


424
Homologue of ferric anguibactin
UniRef100_P94419

Bacillus subtilis

YclO



transport system permerase protein



FatC of V. anguillarum [Bacillus subtilis]


425
Homologue of iron dicitrate transport
UniRef100_P94420

Bacillus subtilis

YclP



ATP-binding protein FecE of E. coli



[Bacillus subtilis]


426
Ferric anguibactin-binding protein
UniRef100_P94421

Bacillus subtilis

YclQ



precusor FatB of V. anguillarum



[Bacillus subtilis]


427
Homologue of multidrug resistance
UniRef100_P94422

Bacillus subtilis

YcnB



protein B, EmrB, of E. coli [Bacillus




subtilis]



428
YcnC protein [Bacillus subtilis]
UniRef100_P94423

Bacillus subtilis

YcnC


429
Hypothetical protein [Bacillus cereus
UniRef100_Q636R0

Bacillus cereus

YqiQ



ZK]

ZK


430
Hypothetical oxidoreductase ycnD
UniRef100_P94424

Bacillus subtilis

YcnD



[Bacillus subtilis]


431
Hypothetical protein ycnE [Bacillus
UniRef100_P94425

Bacillus subtilis





subtilis]



432
YczG protein [Bacillus subtilis]
UniRef100_O31480

Bacillus subtilis



433
Homologue of regulatory protein MocR
UniRef100_P94426

Bacillus subtilis

GabR



of R. meliloti [Bacillus subtilis]


434
Probable 4-aminobutyrate
UniRef100_P94427

Bacillus subtilis

GabT



aminotransferase (EC 2.6.1.19) ((S)-3-



amino-2-methylpropionate



transaminase) [Bacillus subtilis]


435
Cationic amino acid transporter
UniRef100_Q8ESX7

Oceanobacillus

YhdG



[Oceanobacillus iheyensis]


iheyensis



436
Homologue of succinate semialdehyde
UniRef100_P94428

Bacillus subtilis

GabD



dehydrogenase GabD of E. coli



[Bacillus subtilis]


437



YwfM


438


439
YcnI protein [Bacillus subtilis]
UniRef100_P94431

Bacillus subtilis

YcnI


440
Homologue of copper export protein
UniRef100_P94432

Bacillus subtilis

YcnJ



PcoD of E. coli [Bacillus subtilis]


441
YcnK protein [Bacillus subtilis]
UniRef100_P94433

Bacillus subtilis

YcnK


442
Assimilatory nitrate reductase electron
UniRef100_P42433

Bacillus subtilis

NasB



transfer subunit [Bacillus subtilis]


443
Assimilatory nitrate reductase catalytic
UniRef100_P42434

Bacillus subtilis

NasC



subunit [Bacillus subtilis]


444
Nitrite reductase [NAD(P)H] [Bacillus
UniRef100_P42435
NAD(P)H
NasD




subtilis]



445
Assimilatory nitrite reductase
UniRef100_P42436
NAD(P)H
NasE



[NAD(P)H] small subunit [Bacillus




subtilis]



446
Uroporphyrin-III C-methyltransferase
UniRef100_P42437

Bacillus subtilis

NasF



[Bacillus subtilis]


447
Hypothetical transcriptional regulator
UniRef100_O05494

Bacillus subtilis

YdhC



ydhC [Bacillus subtilis]


448
Sodium-dependent transporter
UniRef100_Q8ENE3

Oceanobacillus

YflS



[Oceanobacillus iheyensis]


iheyensis



449
Hypothetical protein ycsD [Bacillus
UniRef100_P42961

Bacillus subtilis





subtilis]



450
UPI00003CC424 UniRef100 entry
UniRef100_UPI00003CC424


451
Possible transcriptional antiterminator,
UniRef100_Q63A16

Bacillus cereus

MtlR



bglG family [Bacillus cereus ZK]

ZK


452
Putative sugar-specific PTS component
UniRef100_Q7X1N9

Lactococcus raffinolactis




EIIB [Lactococcus raffinolactis]


453
SgaT protein [Mannheimia
UniRef100_Q65WA2

Mannheimia





succiniciproducens MBEL55E]



succiniciproducens MBEL55E



454
Transketolase, N-terminal subunit
UniRef100_Q8E202

Streptococcus

Tkt



[Streptococcus agalactiae]


agalactiae



455
Putative transketolase [Salmonella
UniRef100_Q8ZND5

Salmonella

Dxs




typhimurium]



typhimurium



456
Hypothetical protein ycsE [Bacillus
UniRef100_P42962

Bacillus subtilis

YcsE




subtilis]



457
Hypothetical UPF0271 protein ycsF
UniRef100_P42963

Bacillus subtilis

YcsF



[Bacillus subtilis]


458
Hypothetical protein ycsG [Bacillus
UniRef100_P42964

Bacillus subtilis

YcsG




subtilis]



459
Hypothetical UPF0317 protein ycsI
UniRef100_P42966

Bacillus subtilis

YcsI



[Bacillus subtilis]


460
Kinase A inhibitor [Bacillus subtilis]
UniRef100_P60495

Bacillus subtilis

KipI


461
KipI antagonist [Bacillus subtilis]
UniRef100_Q7WY77

Bacillus subtilis

KipA


462
HTH-type transcriptional regulator kipR
UniRef100_P42968

Bacillus subtilis

KipR



[Bacillus subtilis]


463
Hypothetical protein ycsK [Bacillus
UniRef100_P42969

Bacillus subtilis

YcsK




subtilis]



464
PTS system, mannitol-specific IIABC
UniRef100_P42956

Bacillus subtilis

MtlA



component [Bacillus subtilis]


465
Mannitol-1-phosphate 5-
UniRef100_P42957

Bacillus subtilis

MtlD



dehydrogenase [Bacillus subtilis]


466
YdaA protein [Bacillus subtilis]
UniRef100_P96574

Bacillus subtilis

MtlR


467
General stress protein 39 [Bacillus
UniRef100_P80873

Bacillus subtilis

YdaD




subtilis]



468
Hypothetical protein ydaE [Bacillus
UniRef100_P96578

Bacillus subtilis

YdaE




subtilis]



469
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81U55

Bacillus anthracis



470
General stress protein 26 [Bacillus
UniRef100_P80238

Bacillus subtilis

YdaG




subtilis]



471
YdaH protein [Bacillus subtilis]
UniRef100_P96581

Bacillus subtilis

YdaH


472
Lin0463 protein [Listeria innocua]
UniRef100_Q92EJ6

Listeria innocua

YvhJ


473
YdzA protein [Bacillus subtilis]
UniRef100_O31485

Bacillus subtilis



474
BH0424 protein [Bacillus halodurans]
UniRef100_Q9KFQ4

Bacillus halodurans



475
HTH-type transcriptional regulator lrpC
UniRef100_P96582

Bacillus subtilis

LrpC



[Bacillus subtilis]


476
PROBABLE DNA TOPOISOMERASE
UniRef100_P96583

Bacillus subtilis

TopB



III [Bacillus subtilis]


477


478


479


480



YdaO


481



YdaO


482
YdaP protein [Bacillus subtilis]
UniRef100_P96591

Bacillus subtilis

YdaP


483


484
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069


485
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012





anthracis str. A2012]



486


487
Similar to ribosomal-protein-serine N-
UniRef100_Q99WN5

Staphylococcus

YdaF



acetyltransferase [Staphylococcus


aureus





aureus]



488
Manganese transport protein mntH
UniRef100_P96593

Bacillus subtilis

MntH



[Bacillus subtilis]


489


490



AnsB


491



YojK


492
YdaT protein [Bacillus subtilis]
UniRef100_P96595

Bacillus subtilis

YdaT


493
Hypothetical protein ydbA [Bacillus
UniRef100_P96596

Bacillus subtilis

YdbA




subtilis]



494
Na+/H+ antiporter NhaC [Bacillus
UniRef100_Q81FX8

Bacillus cereus

NhaC




cereus]



495
YdbB protein [Bacillus subtilis]
UniRef100_P96597

Bacillus subtilis

YdbB


496
Glucose starvation-inducible protein B
UniRef100_P26907

Bacillus subtilis

GsiB



[Bacillus subtilis]


497
Hypothetical UPF0118 protein ydbI
UniRef100_P96604

Bacillus subtilis

YdbI



[Bacillus subtilis]


498



GltT


499
YdbJ protein [Bacillus subtilis]
UniRef100_P96605

Bacillus subtilis

YdbJ


500
YdbK protein [Bacillus subtilis]
UniRef100_P96606

Bacillus subtilis

YdbK


501
Hypothetical protein ydbL [Bacillus
UniRef100_P96607

Bacillus subtilis

YdbL




subtilis]



502
YdbM protein [Bacillus subtilis]
UniRef100_P96608

Bacillus subtilis

YdbM


503


504


505
YdbP protein [Bacillus subtilis]
UniRef100_P96611

Bacillus subtilis

YdbP


506
D-alanine--D-alanine ligase [Bacillus
UniRef100_P96612

Bacillus subtilis

Ddl




subtilis]



507



MurF


508
Esterase [Oceanobacillus iheyensis]
UniRef100_Q8ESM0

Oceanobacillus

YvaK






iheyensis



509
YdbR protein [Bacillus subtilis]
UniRef100_P96614

Bacillus subtilis

YdbR


510
YdbS protein [Bacillus subtilis]
UniRef100_P96615

Bacillus subtilis

YdbS


511
YdbT protein [Bacillus subtilis]
UniRef100_P96616

Bacillus subtilis

YdbT


512
YdcA protein [Bacillus subtilis]
UniRef100_P96617

Bacillus subtilis

YdcA


513



YdcC


514



Alr


515


516
YdcE protein [Bacillus subtilis]
UniRef100_P96622

Bacillus subtilis

YdcE


517



RsbR


518



RsbS


519



RsbT


520



RsbU


521



RsbV


522



RsbW


523



SigB


524



RsbX


525
YdcI protein [Bacillus subtilis]
UniRef100_O31489

Bacillus subtilis

YdcI


526
Transcriptional regulator, TetR family
UniRef100_Q6HGY5

Bacillus

YxbF



[Bacillus thuringiensis]


thuringiensis



527
Lin1189 protein [Listeria innocua]
UniRef100_Q92CI2

Listeria innocua

YdgH


528


529
Protein sprT-like [Bacillus subtilis]
UniRef100_P96628

Bacillus subtilis

YdcK


530
Possible transporter, EamA family
UniRef100_Q638K5

Bacillus cereus ZK




[Bacillus cereus ZK]


531


532
Hypothetical protein ORF00034
UniRef100_O87235

Lactococcus lactis




[Lactococcus lactis]


533
Delta5 acyl-lipid desaturase [Bacillus
UniRef100_Q81C02

Bacillus cereus

Des




cereus]



534
Cold shock protein cspC [Bacillus
UniRef100_P39158

Bacillus subtilis





subtilis]



535



YogA


536
Membrane protein, putative [Bacillus
UniRef100_Q734Y0

Bacillus cereus

YyaS




cereus]



537
Transcriptional regulator, MarR family
UniRef100_Q734X9

Bacillus cereus

YybA



[Bacillus cereus]


538
Acetyltransferase, GNAT family
UniRef100_Q734X8

Bacillus cereus

PaiA



[Bacillus cereus]


539
Protease synthase and sporulation
UniRef100_Q734X7

Bacillus cereus

PaiB



negative regulatory protein PAI 2



[Bacillus cereus]


540
BH0654 protein [Bacillus halodurans]
UniRef100_Q9KF33

Bacillus

RocF






halodurans



541


542
YdeO protein [Bacillus subtilis]
UniRef100_P96672

Bacillus subtilis

YdeO


543
Transporter, LysE family [Bacillus
UniRef100_Q81D17

Bacillus cereus

YrhP




cereus]



544



YwqM


545


546
Permease, putative [Bacillus anthracis]
UniRef100_Q81QY7

Bacillus

YvqJ






anthracis



547
Putative cyclase [Rhodopseudomonas
UniRef100_Q6N497

Rhodopseudomonas palustris





palustris]



548
Hypothetical transport protein ydgF
UniRef100_P96704

Bacillus subtilis

YdgF



[Bacillus subtilis]


549


550



YknW


551
RNA polymerase sigma factor sigV
UniRef100_O05404

Bacillus subtilis

SigV



[Bacillus subtilis]


552
Putative anti-SigV factor [Bacillus
UniRef100_O05403

Bacillus subtilis

YrhM




subtilis]



553
Hypothetical protein yrhL [Bacillus
UniRef100_O05402

Bacillus subtilis

YrhL




subtilis]



554



YdgK


555



YwpD


556



LytT


557
Collagen adhesion protein [Bacillus
UniRef100_Q630P2

Bacillus cereus ZK





cereus ZK]



558
Lin0929 protein [Listeria innocua]
UniRef100_Q92D88

Listeria innocua



559


560
Metabolite transport protein [Bacillus
UniRef100_O34718

Bacillus subtilis

YdjK




subtilis]



561
Thiamine-monophosphate kinase
UniRef100_O05514

Bacillus subtilis

ThiL



[Bacillus subtilis]


562
Hypothetical UPF0079 protein ydiB
UniRef100_O05515

Bacillus subtilis

YdiB



[Bacillus subtilis]


563
YdiC protein [Bacillus subtilis]
UniRef100_O05516

Bacillus subtilis

YdiC


564
YdiD protein [Bacillus subtilis]
UniRef100_O05517

Bacillus subtilis

YdiD


565
Probable O-sialoglycoprotein
UniRef100_O05518

Bacillus subtilis

Gcp



endopeptidase [Bacillus subtilis]


566



YdiF


567



YdiF


568
Molybdenum cofactor biosynthesis
UniRef100_O05520

Bacillus subtilis

YdiG



protein C [Bacillus subtilis]


569
Redox-sensing transcriptional repressor
UniRef100_O05521

Bacillus subtilis

YdiH



rex [Bacillus subtilis]


570
YdiI [Bacillus halodurans]
UniRef100_Q9Z9P5

Bacillus halodurans



571



TatCY


572
Hypothetical lipoprotein ydiK precursor
UniRef100_O05524

Bacillus subtilis




[Bacillus subtilis]


573



YdiL


574
10 kDa chaperonin [Bacillus subtilis]
UniRef100_P28599

Bacillus subtilis



575
60 kDa chaperonin [Bacillus subtilis]
UniRef100_P28598

Bacillus subtilis

GroEL


576


577


578
Hypothetical protein yolD
UniRef100_O64030
Bacteriophage SPBc2



[Bacteriophage SPBc2]


579


580


581
YoaR [Bacillus subtilis]
UniRef100_O34611

Bacillus subtilis

YoaR


582
Hypothetical protein yfmQ [Bacillus
UniRef100_O06475

Bacillus subtilis

YfmQ




subtilis]



583


584
YoqW protein [Bacteriophage SPBc2]
UniRef100_O64131
Bacteriophage
YoqW





SPBc2


585
Lin2076 protein [Listeria innocua]
UniRef100_Q92A46

Listeria innocua

YerO


586
PEP synthase [Bacillus subtilis]
UniRef100_O34309

Bacillus subtilis

Pps


587
Hypothetical protein yoaF [Bacillus
UniRef100_O31829

Bacillus subtilis





subtilis]



588
Hypothetical protein [Staphylococcus
UniRef100_Q6GK76

Staphylococcus aureus





aureus]



589
Short-chain dehydrodenase
UniRef100_Q97LM1

Clostridium

DltE



[Clostridium acetobutylicum]


acetobutylicum



590
Type B carboxylesterase [Bacillus sp.
UniRef100_Q9L378

Bacillus sp. BP-7

PnbA



BP-7]


591
Inositol transport protein
UniRef100_Q8ESX2

Oceanobacillus

IolF



[Oceanobacillus iheyensis]


iheyensis



592
Phage shock protein A homolog
UniRef100_P54617

Bacillus subtilis

PspA



[Bacillus subtilis]


593
YdjG protein [Bacillus subtilis]
UniRef100_O34434

Bacillus subtilis

YdjG


594
YdjH protein [Bacillus subtilis]
UniRef100_O35004

Bacillus subtilis

YdjH


595
YdjI protein [Bacillus subtilis]
UniRef100_O34789

Bacillus subtilis

YdjI


596
Putative oxidoreductase
UniRef100_Q67S08

Symbiobacterium

YtmO



[Symbiobacterium thermophilum]


thermophilum



597
YrhO [Bacillus subtilis]
UniRef100_O05405

Bacillus subtilis

YrhO


598



YrhP


599
Helix-turn-helix domain protein [Bacillus
UniRef100_Q73C00

Bacillus cereus





cereus]



600
Stage V sporulation protein E [Bacillus
UniRef100_Q9K7T4

Bacillus

SpoVE




halodurans]



halodurans



601
Stage V sporulation protein E [Bacillus
UniRef100_Q9K7T3

Bacillus

FtsW




halodurans]



halodurans



602


603
Hypothetical protein [Bacillus cereus]
UniRef100_Q72Z89

Bacillus cereus



604
BH1889 protein [Bacillus halodurans]
UniRef100_Q9KBN6

Bacillus

YobV






halodurans



605



YjeA


606



YjeA


607



TreA


608
Putative HTH-type transcriptional
UniRef100_O06987

Bacillus subtilis

YvdE



regulator yvdE [Bacillus subtilis]


609
Hypothetical protein yvdF [Bacillus
UniRef100_O06988

Bacillus subtilis

YvdF




subtilis]



610
Hypothetical protein yvdG [Bacillus
UniRef100_O06989

Bacillus subtilis

YvdG




subtilis]



611
Hypothetical protein yvdH [Bacillus
UniRef100_O06990

Bacillus subtilis

YvdH




subtilis]



612
Hypothetical protein yvdI [Bacillus
UniRef100_O06991

Bacillus subtilis

YvdI




subtilis]



613
Hypothetical protein yvdJ [Bacillus
UniRef100_O06992

Bacillus subtilis

YvdJ




subtilis]



614
Hypothetical glycosyl hydrolase yvdK
UniRef100_O06993

Bacillus subtilis

YvdK



[Bacillus subtilis]


615
Oligo-1,6-glucosidase [Bacillus subtilis]
UniRef100_O06994

Bacillus subtilis

MalL


616
Putative beta-phosphoglucomutase
UniRef100_O06995

Bacillus subtilis

PgcM



[Bacillus subtilis]


617
Tyrosyl-tRNA synthetase 2 [Bacillus
UniRef100_P25151

Bacillus subtilis

TyrZ




subtilis]



618
Putative HTH-type transcriptional
UniRef100_P25150

Bacillus subtilis

YwaE



regulator ywaE [Bacillus subtilis]


619


620
Hypothetical protein SE2399
UniRef100_Q8CQM7

Staphylococcus epidermidis




[Staphylococcus epidermidis]


621
Hypothetical protein ydjM [Bacillus
UniRef100_P40775

Bacillus subtilis

YdjM




subtilis]



622
YdjN protein [Bacillus subtilis]
UniRef100_O34353

Bacillus subtilis

YdjN


623
Hypothetical protein [Bacillus cereus]
UniRef100_Q81AT6

Bacillus cereus

YeaA


624


625
Signal peptidase I [Bacillus cereus]
UniRef100_Q73C25

Bacillus cereus

SipS


626


627
Hypothetical protein yhfK [Bacillus
UniRef100_O07609

Bacillus subtilis

YhfK




subtilis]



628
Transcriptional regulator [Bacillus
UniRef100_Q9K766

Bacillus

YdeE




halodurans]



halodurans



629
Spore coat protein A [Bacillus subtilis]
UniRef100_P07788

Bacillus subtilis

CotA


630



YkrP


631
Extracellular protein [Lactobacillus
UniRef100_Q88T27

Lactobacillus

YcdA




plantarum]



plantarum



632
Hypothetical UPF0018 protein yeaB
UniRef100_P46348

Bacillus subtilis

YeaB



[Bacillus subtilis]


633
YeaC [Bacillus subtilis]
UniRef100_P94474

Bacillus subtilis

YeaC


634
Hypothetical protein [Bacillus cereus]
UniRef100_Q739D8

Bacillus cereus

YeaD


635
YebA [Bacillus subtilis]
UniRef100_P94476

Bacillus subtilis

YebA


636
GMP synthase [glutamine-hydrolyzing]
UniRef100_P29727
glutamine-
GuaA



[Bacillus subtilis]

hydrolyzing


637
Hypoxanthine/guanine permease
UniRef100_O34987

Bacillus subtilis

PbuG



[Bacillus subtilis]


638



YebC


639
Hypothetical UPF0316 protein yebE
UniRef100_O34624

Bacillus subtilis

YebE



[Bacillus subtilis]


640
YebG protein [Bacillus subtilis]
UniRef100_O34700

Bacillus subtilis



641
Phosphoribosylaminoimidazole
UniRef100_P12044

Bacillus subtilis

PurE



carboxylase catalytic subunit [Bacillus




subtilis]



642



PurK


643
Adenylosuccinate lyase [Bacillus
UniRef100_P12047

Bacillus subtilis

PurB




subtilis]



644
Phosphoribosylaminoimidazole-
UniRef100_P12046

Bacillus subtilis

PurC



succinocarboxamide synthase [Bacillus




subtilis]



645
Hypothetical UPF0062 protein yexA
UniRef100_P12049

Bacillus subtilis




[Bacillus subtilis]


646
Phosphoribosylformylglycinamidine
UniRef100_P12041

Bacillus subtilis

PurQ



synthase I [Bacillus subtilis]


647
Phosphoribosylformylglycinamidine
UniRef100_P12042

Bacillus subtilis

PurL



synthase II [Bacillus subtilis]


648
Amidophosphoribosyltransferase
UniRef100_P00497

Bacillus subtilis

PurF



precursor [Bacillus subtilis]


649



PurM


650
Phosphoribosylglycinamide
UniRef100_P12040

Bacillus subtilis

PurN



formyltransferase [Bacillus subtilis]


651
Bifunctional purine biosynthesis protein
UniRef100_P12048
Includes:
PurH



purH [Includes:

Phosphoribosylaminoimidazolecarboxamide



Phosphoribosylaminoimidazolecarboxamide

formyltransferase



formyltransferase (EC 2.1.2.3)

(EC 2.1.2.3)



(AICAR transformylase); IMP

(AICAR



cyclohydrolase (EC 3.5.4.10)

transformylase);



(Inosinicase) (IMP synthetase) (ATIC)]

IMP



[Bacillus subtilis]

cyclohydrolase





(EC 3.5.4.10)





(Inosinicase)





(IMP synthetase)





(ATIC)


652
Phosphoribosylamine--glycine ligase
UniRef100_P12039

Bacillus subtilis

PurD



[Bacillus subtilis]


653



YxbF


654
Putative cytochrome P450 yjiB [Bacillus
UniRef100_O34374

Bacillus subtilis

YjiB




subtilis]



655


656
Hypothetical lipoprotein yybP precursor
UniRef100_P37488

Bacillus subtilis

YybP



[Bacillus subtilis]


657
Transposase [Thermoanaerobacter
UniRef100_Q8RCM3

Thermoanaerobacter





tengcongensis]



tengcongensis



658
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81ZG4

Bacillus anthracis



659
Putative adenine deaminase yerA
UniRef100_O34909

Bacillus subtilis

YerA



[Bacillus subtilis]


660
YerB protein [Bacillus subtilis]
UniRef100_O34968

Bacillus subtilis

YerB


661
YecD [Bacillus subtilis]
UniRef100_Q7BVT7

Bacillus subtilis

YerC


662
PcrB protein homolog [Bacillus subtilis]
UniRef100_O34790

Bacillus subtilis

PcrB


663
ATP-dependent DNA helicase pcrA
UniRef100_O34580

Bacillus subtilis

PcrA



[Bacillus subtilis]


664
DNA ligase [Bacillus subtilis]
UniRef100_O31498

Bacillus subtilis

LigA


665
YerH protein [Bacillus subtilis]
UniRef100_O34629

Bacillus subtilis

YerH


666
BH0586 protein [Bacillus halodurans]
UniRef100_Q9KF99

Bacillus halodurans



667


668


669
BH0589 protein [Bacillus halodurans]
UniRef100_Q9KF96

Bacillus halodurans



670
Phosphotriesterase homology protein
UniRef100_P45548

Escherichia coli




[Escherichia coli]


671
Similar to unknown protein YhfS of
UniRef100_Q7N5F2

Photorhabdus

Csd




Escherichia coli [Photorhabdus



luminescens





luminescens]



672
Phosphopentomutase
UniRef100_Q67S06

Symbiobacterium

Drm



[Symbiobacterium thermophilum]


thermophilum



673
Putative alanine racemase
UniRef100_Q67S05

Symbiobacterium




[Symbiobacterium thermophilum]


thermophilum



674
Glucosamine-6-phosphate deaminase
UniRef100_Q8ESL6

Oceanobacillus

NagB



[Oceanobacillus iheyensis]


iheyensis



675
SapB protein [Bacillus subtilis]
UniRef100_Q45514

Bacillus subtilis

SapB


676



OpuE


677
Glutamyl-tRNA(Gln) amidotransferase
UniRef100_O06492

Bacillus subtilis




subunit C [Bacillus subtilis]


678
Glutamyl-tRNA(Gln) amidotransferase
UniRef100_O06491

Bacillus subtilis

GatA



subunit A [Bacillus subtilis]


679



GatB


680
Hypothetical protein [Bacillus
UniRef100_Q848Y2

Bacillus megaterium





megaterium]



681


682



YdhT


683


684
Putative HTH-type transcriptional
UniRef100_O31500

Bacillus subtilis

YerO



regulator yerO [Bacillus subtilis]


685
Swarming motility protein swrC [Bacillus
UniRef100_O31501

Bacillus subtilis

YerP




subtilis]



686



YjcK


687
Inosine-uridine preferring nucleoside
UniRef100_Q81DM6

Bacillus cereus




hydrolase [Bacillus cereus]


688
YerQ protein [Bacillus subtilis]
UniRef100_O31502

Bacillus subtilis

YerQ


689
Hypothetical RNA methyltransferase
UniRef100_O31503

Bacillus subtilis

YefA



yefA [Bacillus subtilis]


690
Type I restriction-modification system
UniRef100_Q817S1

Bacillus cereus




specificity subunit [Bacillus cereus]


691
Type I restriction-modification system
UniRef100_Q817S2

Bacillus cereus




methylation subunit [Bacillus cereus]


692
Type IC specificity subunit
UniRef100_Q9RNW0

Streptococcus thermophilus




[Streptococcus thermophilus]


693
Type I restriction-modification system
UniRef100_Q817S4

Bacillus cereus




restriction subunit [Bacillus cereus]


694


695
Beta-glucosides PTS, EIIBCA
UniRef100_Q88T54

Lactobacillus

BglP



[Lactobacillus plantarum]


plantarum



696
6-phospho-beta-glucosidase
UniRef100_Q88T55

Lactobacillus

BglH



[Lactobacillus plantarum]


plantarum



697



LicT


698
Response regulator aspartate
UniRef100_O34930

Bacillus subtilis

RapK



phosphatase K [Bacillus subtilis]


699


700
Methyltransferase [Bacillus cereus ZK]
UniRef100_Q639N2

Bacillus cereus ZK



701
Hypothetical UPF0082 protein
UniRef100_P62032

Bacillus cereus

YeeI



BCE0595 [Bacillus cereus]


702
Putative HTH-type transcriptional
UniRef100_O28646

Archaeoglobus fulgidus




regulator AF1627 [Archaeoglobus




fulgidus]



703


704
YfmT [Bacillus subtilis]
UniRef100_O06478

Bacillus subtilis

YfmT


705
YfmS [Bacillus subtilis]
UniRef100_O06477

Bacillus subtilis

YfmS


706



YflS


707
YfmR [Bacillus subtilis]
UniRef100_O06476

Bacillus subtilis

YfmR


708


709
YciA protein [Bacillus subtilis]
UniRef100_P94398

Bacillus subtilis

YciA


710
UPI00002BDF65 UniRef100 entry
UniRef100_UPI00002BDF65

YpdA


711
Ferrusion transporter protein
UniRef100_Q6U5S9

Klebsiella pneumoniae




[Klebsiella pneumoniae]


712


713
YciC protein [Bacillus
UniRef100_Q70KK8

Bacillus

YciC




amyloliquefaciens]



amyloliquefaciens



714



BioW


715
Adenosylmethionine-8-amino-7-
UniRef100_P53555

Bacillus subtilis

BioA



oxononanoate aminotransferase



[Bacillus subtilis]


716
8-amino-7-oxononanoate synthase
UniRef100_P53556

Bacillus subtilis

BioF



[Bacillus subtilis]


717
BioD protein [Bacillus
UniRef100_Q70JZ0

Bacillus

BioD




amyloliquefaciens]



amyloliquefaciens



718
BioB protein [Bacillus
UniRef100_Q70JZ1

Bacillus

BioB




amyloliquefaciens]



amyloliquefaciens



719
BioI protein [Bacillus amyloliquefaciens]
UniRef100_Q70JZ2

Bacillus

BioI






amyloliquefaciens



720
BH1501 protein [Bacillus halodurans]
UniRef100_Q9KCR8

Bacillus halodurans



721



YfmP


722
Multidrug efflux protein yfmO [Bacillus
UniRef100_O06473

Bacillus subtilis

YfmO




subtilis]



723
YfmM protein [Bacillus subtilis]
UniRef100_O34512

Bacillus subtilis

YfmM


724
YfmL protein [Bacillus subtilis]
UniRef100_O34750

Bacillus subtilis

YfmL


725
YfmJ protein [Bacillus subtilis]
UniRef100_O34812

Bacillus subtilis

YfmJ


726
Hypothetical protein yfmB [Bacillus
UniRef100_O34626

Bacillus subtilis

YfmB




subtilis]



727
General stress protein 17M [Bacillus
UniRef100_P80241

Bacillus subtilis

YflT




subtilis]



728



YflS


729



YflS


730
Putative permease [Clostridium tetani]
UniRef100_Q895A0

Clostridium tetani



731
Possible Zn-dependent hydrolase,
UniRef100_Q6HKH5

Bacillus

YqgX



beta-lactamase superfamily [Bacillus


thuringiensis





thuringiensis]



732
YflN protein [Bacillus subtilis]
UniRef100_O34409

Bacillus subtilis

YflN


733
Zink-carboxypeptidase [Clostridium
UniRef100_Q898E1

Clostridium tetani





tetani]



734
Nitric oxide synthase oxygenase
UniRef100_O34453

Bacillus subtilis

YflM



[Bacillus subtilis]


735
Membrane protein, putative [Bacillus
UniRef100_Q72YN4

Bacillus cereus

YvaZ




cereus]



736
Transcriptional regulator, ArsR family
UniRef100_Q632K6

Bacillus cereus ZK




[Bacillus cereus ZK]


737
Putative acylphosphatase [Bacillus
UniRef100_O35031

Bacillus subtilis





subtilis]



738
YflK protein [Bacillus subtilis]
UniRef100_O34542

Bacillus subtilis

YflK


739


740


741
YflG protein [Bacillus subtilis]
UniRef100_O34484

Bacillus subtilis

YflG


742



YflE


743
Hypothetical protein yflB [Bacillus
UniRef100_O34887

Bacillus subtilis





subtilis]



744



YflA


745
Probable PTS system, trehalose-
UniRef100_P39794

Bacillus subtilis

TreP



specific IIBC component [Bacillus




subtilis]



746
Alpha-glucosidase [Bacillus sp.
UniRef100_Q9L872

Bacillus sp.

TreA



DG0303]

DG0303


747
Trehalose operon transcriptional
UniRef100_P39796

Bacillus subtilis

TreR



repressor [Bacillus subtilis]


748
Acetyltransferases
UniRef100_Q8RBZ9

Thermoanaerobacter

YvfD



[Thermoanaerobacter tengcongensis]


tengcongensis



749


750


751
Predicted pyridoxal phosphate-
UniRef100_Q8RBY7

Thermoanaerobacter

SpsC



dependent enzyme apparently involved


tengcongensis




in regulation of cell wall biogenesis



[Thermoanaerobacter tengcongensis]


752



YkuQ


753
UPI000029FB28 UniRef100 entry
UniRef100_UPI000029FB28

YtcB


754
Hypothetical protein MA2181
UniRef100_Q8TNU7

Methanosarcina acetivorans




[Methanosarcina acetivorans]


755


756
Beta 1,4 glucosyltransferase [Bacillus
UniRef100_Q81GJ1

Bacillus cereus

YolJ




cereus]



757
Predicted pyridoxal phosphate-
UniRef100_Q8RC02

Thermoanaerobacter

SpsC



dependent enzyme apparently involved


tengcongensis




in regulation of cell wall biogenesis



[Thermoanaerobacter tengcongensis]


758
Predicted dehydrogenases and related
UniRef100_Q8RC00

Thermoanaerobacter

YrbE



proteins [Thermoanaerobacter


tengcongensis





tengcongensis]



759
UDP-glucose: GDP-mannose
UniRef100_Q8CXB6

Oceanobacillus

TuaD



dehydrogenase [Oceanobacillus


iheyensis





iheyensis]



760
Hypothetical protein [Bacillus cereus
UniRef100_Q635H5

Bacillus cereus

YqkD



ZK]

ZK


761
Hypothetical UPF0087 protein ydeP
UniRef100_P96673

Bacillus subtilis

YdeP



[Bacillus subtilis]


762
Putative NAD(P)H nitroreductase yfkO
UniRef100_O34475

Bacillus subtilis

YfkO



[Bacillus subtilis]


763



YfkN


764
General stress protein 18 [Bacillus
UniRef100_P80876

Bacillus subtilis

YfkM




subtilis]



765
YfkK protein [Bacillus subtilis]
UniRef100_O35019

Bacillus subtilis



766
Amino acid transporter [Bacillus
UniRef100_Q9K5Q5

Bacillus

YflA




halodurans]



halodurans



767
YfkJ protein [Bacillus subtilis]
UniRef100_O35016

Bacillus subtilis

YfkJ


768
Hypothetical protein yfkI precursor
UniRef100_O34418

Bacillus subtilis

YfkI



[Bacillus subtilis]


769
YfkH protein [Bacillus subtilis]
UniRef100_O34437

Bacillus subtilis

YfkH


770
YfkF protein [Bacillus subtilis]
UniRef100_O34929

Bacillus subtilis

YfkF


771
Hypothetical conserved protein
UniRef100_Q8ELS1

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


772
YfkE protein [Bacillus subtilis]
UniRef100_O34840

Bacillus subtilis

YfkE


773
YfkD protein [Bacillus subtilis]
UniRef100_O34579

Bacillus subtilis

YfkD


774
Thioredoxin-like oxidoreductases
UniRef100_Q81IC7

Bacillus cereus

YfkA



[Bacillus cereus]


775
YfjT protein [Bacillus subtilis]
UniRef100_O35041

Bacillus subtilis



776



YfjS


777
UPI000029390C UniRef100 entry
UniRef100_UPI000029390C

AraM


778


779
YfjQ protein [Bacillus subtilis]
UniRef100_O31543

Bacillus subtilis

YfjQ


780
YfjP protein [Bacillus subtilis]
UniRef100_O31544

Bacillus subtilis

YfjP


781



YfjO


782
YfjM protein [Bacillus subtilis]
UniRef100_O31547

Bacillus subtilis

YfjM


783


784
Hypothetical protein yfjL [Bacillus
UniRef100_P40773

Bacillus subtilis

YfjL




subtilis]



785
UPI00003CB259 UniRef100 entry
UniRef100_UPI00003CB259

YvkB


786
YdhE protein [Bacillus subtilis]
UniRef100_O05496

Bacillus subtilis

YdhE


787
Hypothetical protein yckD precursor
UniRef100_P42402

Bacillus subtilis




[Bacillus subtilis]


788
Hypothetical metabolite transport
UniRef100_O34691

Bacillus subtilis

YceI



protein yceI [Bacillus subtilis]


789



SacX


790
Levansucrase and sucrase synthesis
UniRef100_P15401

Bacillus subtilis

SacY



operon antiterminator [Bacillus subtilis]


791
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81NL1

Bacillus

YbcF





anthracis


792
Hypothetical protein ybcD [Bacillus
UniRef100_Q639F7

Bacillus cereus

YbcD




cereus ZK]


ZK


793
Potential NADH-quinone
UniRef100_P39755

Bacillus subtilis

NdhF



oxidoreductase subunit 5 [Bacillus




subtilis]



794
YbcI protein [Bacillus subtilis]
UniRef100_O34380

Bacillus subtilis

YbcI


795



YraA


796
TPP-dependent acetoin dehydrogenase
UniRef100_Q81PM6

Bacillus

AcoA



E1 alpha-subunit [Bacillus anthracis]


anthracis



797
TPP-dependent acetoin dehydrogenase
UniRef100_Q736U7

Bacillus cereus

AcoB



E1 beta-subunit [Bacillus cereus]


798
Dihydrolipoyllysine-residue
UniRef100_O31550

Bacillus subtilis

AcoC



acetyltransferase component of acetoin



cleaving system [Bacillus subtilis]


799
Dihydrolipoyl dehydrogenase [Bacillus
UniRef100_O34324

Bacillus subtilis

AcoL




subtilis]



800
Acetoin operon transcriptional activator,
UniRef100_Q736V6

Bacillus cereus

AcoR



putative [Bacillus cereus]


801
Hypothetical UPF0060 protein yfjF
UniRef100_O31553

Bacillus subtilis

YfjF



[Bacillus subtilis]


802
Maltose-6′-phosphate glucosidase
UniRef100_P54716

Bacillus subtilis

MalA



[Bacillus subtilis]


803
HTH-type transcriptional regulator glvR
UniRef100_P54717

Bacillus subtilis

YfiA



[Bacillus subtilis]


804
PTS system, arbutin-like IIBC
UniRef100_P54715

Bacillus subtilis

MalP



component [Bacillus subtilis]


805
UPI00003651CF UniRef100 entry
UniRef100_UPI00003651CF


806
UPI000034AA1D UniRef100 entry
UniRef100_UPI000034AA1D


807
Transcriptional regulator, MarR family
UniRef100_Q638I2

Bacillus cereus

YvaP



[Bacillus cereus ZK]

ZK


808



YfiD


809
Hypothetical protein yfiE [Bacillus
UniRef100_P54721

Bacillus subtilis

YfiE




subtilis]



810
Xylosidase/arabinosidase [Bacteroides
UniRef100_Q8A036

Bacteroides

XynB




thetaiotaomicron]



thetaiotaomicron



811
Xylan beta-1,4-xylosidase [Bacillus
UniRef100_Q9K6P5

Bacillus

XynB




halodurans]



halodurans



812
Trancriptional regulator of AraC family
UniRef100_Q97FW8

Clostridium

YbfI



[Clostridium acetobutylicum]


acetobutylicum



813



YtcQ


814
NAD(P)H dehydrogenase, quinone
UniRef100_Q638S8

Bacillus cereus ZK




family [Bacillus cereus ZK]


815
Mutator MutT protein [Bacillus
UniRef100_Q9K8B7

Bacillus

YjhB




halodurans]



halodurans



816
YfiT protein [Bacillus subtilis]
UniRef100_O31562

Bacillus subtilis

YfiT


817
YfiX [Bacillus subtilis]
UniRef100_O52961

Bacillus subtilis

YfiX


818
Hypothetical protein yfhB [Bacillus
UniRef100_O31570

Bacillus subtilis

YfhB




subtilis]



819
YfhC protein [Bacillus subtilis]
UniRef100_O31571

Bacillus subtilis

YfhC


820
Hypothetical protein yfhD [Bacillus
UniRef100_O31572

Bacillus subtilis





subtilis]



821


822
BH0923 homolog [Bacillus cereus]
UniRef100_Q81IA0

Bacillus cereus



823
Hypothetical UPF0105 protein yfhF
UniRef100_O31574

Bacillus subtilis

YfhF



[Bacillus subtilis]


824
Regulatory protein recX [Bacillus
UniRef100_O31575

Bacillus subtilis

YfhG




subtilis]



825
YfhH protein [Bacillus subtilis]
UniRef100_O31576

Bacillus subtilis

YfhH


826


827
YfhJ protein [Bacillus subtilis]
UniRef100_O31578

Bacillus subtilis



828
CsbB protein [Bacillus subtilis]
UniRef100_Q45539

Bacillus subtilis

CsbB


829
Hypothetical protein SE1997
UniRef100_Q8CR87

Staphylococcus epidermidis




[Staphylococcus epidermidis]


830
YfhO protein [Bacillus subtilis]
UniRef100_O31582

Bacillus subtilis

YfhO


831
YfhP protein [Bacillus subtilis]
UniRef100_O31583

Bacillus subtilis

YfhP


832
YfhQ protein [Bacillus subtilis]
UniRef100_O31584

Bacillus subtilis

YfhQ


833
YfhS protein [Bacillus subtilis]
UniRef100_O31585

Bacillus subtilis



834
Unidentfied dehydrogenase [Bacillus
UniRef100_P71079

Bacillus subtilis

FabL




subtilis]



835


836
Hypothetical protein ygaB [Bacillus
UniRef100_P71080

Bacillus subtilis





subtilis]



837
YgaC protein [Bacillus subtilis]
UniRef100_Q796Z1

Bacillus subtilis

YgaC


838
Unidentified transporter-ATP binding
UniRef100_P71082

Bacillus subtilis

YgaD



[Bacillus subtilis]


839
Oligopeptide ABC transporter [Bacillus
UniRef100_Q9K6T0

Bacillus

AppD




halodurans]



halodurans



840
Oligopeptide ABC transporter [Bacillus
UniRef100_Q9K6T1

Bacillus

AppF




halodurans]



halodurans



841
Dipeptide transporter protein DppA
UniRef100_P94310

Bacillus firmus

OppA



[Bacillus firmus]


842
Dipeptide ABC transporter [Bacillus
UniRef100_Q9K6T3

Bacillus

AppB




halodurans]



halodurans



843
Dipeptide transport system permease
UniRef100_P94312

Bacillus

AppC



protein dppC [Bacillus pseudofirmus]


pseudofirmus



844
Hypothetical 40.7 kd protein [Bacillus
UniRef100_P71083

Bacillus subtilis

YgaE




subtilis]



845
Glutamate-1-semialdehyde 2,1-
UniRef100_P71084

Bacillus subtilis

GsaB



aminomutase 2 [Bacillus subtilis]


846
YgaF protein [Bacillus subtilis]
UniRef100_Q796Y8

Bacillus subtilis

YgaF


847
Peroxide operon regulator [Bacillus
UniRef100_P71086

Bacillus subtilis

PerR




subtilis]



848


849
Hypothetical protein ygxA [Bacillus
UniRef100_Q04385

Bacillus subtilis

YgxA




subtilis]



850



RapD


851


852


853


854


855


856
Hypothetical protein [Bacillus
UniRef100_Q6HH72

Bacillus thuringiensis





thuringiensis]



857



YxiD


858
Hypothetical protein [uncultured
UniRef100_Q64DF2
uncultured archaeon



archaeon GZfos18F2]

GZfos18F2


859


860



YxiD


861



RapE


862
Putative membrane protein
UniRef100_Q82NM0

Streptomyces avermitilis




[Streptomyces avermitilis]


863
3-dehydroquinate dehydratase [Listeria
UniRef100_Q8Y9N4

Listeria

AroC




monocytogenes]



monocytogenes



864



ThiC


865
Putative aliphatic sulfonates transport
UniRef100_P97027

Bacillus subtilis

SsuB



ATP-binding protein ssuB [Bacillus




subtilis]



866
Putative aliphatic sulfonates binding
UniRef100_P40400

Bacillus subtilis

SsuA



protein precursor [Bacillus subtilis]


867
Putative aliphatic sulfonates transport
UniRef100_P40401

Bacillus subtilis

SsuC



permease protein ssuC [Bacillus




subtilis]



868



SsuD


869



SsuD


870
Hypothetical lipoprotein ygaO precursor
UniRef100_P97029

Bacillus subtilis

YgaO



[Bacillus subtilis]


871
DNA-binding protein [Bacillus anthracis]
UniRef100_Q81V18

Bacillus anthracis



872


873
ABC-type multidrug transport system,
UniRef100_Q8RBH0

Thermoanaerobacter

YhaQ



ATPase component


tengcongensis




[Thermoanaerobacter tengcongensis]


874


875


876


877


878
30S ribosomal protein S14-2 [Bacillus
UniRef100_O31587

Bacillus subtilis





subtilis]



879
Hypothetical protein yhzB [Bacillus
UniRef100_O31588

Bacillus subtilis

YhzB




subtilis]



880
Hypothetical 48.5 kd protein [Bacillus
UniRef100_P97030

Bacillus subtilis

YhbA




subtilis]



881
Hypothetical 35.8 kd protein [Bacillus
UniRef100_P97031

Bacillus subtilis

YhbB




subtilis]



882
CspR [Bacillus subtilis]
UniRef100_Q45512

Bacillus subtilis

CspR


883
Hypothetical 27 kd protein [Bacillus
UniRef100_P97032

Bacillus subtilis

YhbD




subtilis]



884
Hypothetical Cytosolic Protein [Bacillus
UniRef100_Q813H1

Bacillus cereus

YhbE




cereus]



885
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81PD2

Bacillus

YhbF






anthracis



886


887
PrkA protein [Bacillus subtilis]
UniRef100_P39134

Bacillus subtilis

PrkA


888
Stress response UPF0229 protein yhbH
UniRef100_P45742

Bacillus subtilis

YhbH



[Bacillus subtilis]


889
YhbJ protein [Bacillus subtilis]
UniRef100_O31593

Bacillus subtilis

YhbJ


890
Hypothetical transport protein yhcA
UniRef100_P54585

Bacillus subtilis

YhcA



[Bacillus subtilis]


891
Hypothetical protein yhcB [Bacillus
UniRef100_P54586

Bacillus subtilis

YhcB




subtilis]



892



YhcC


893


894
Hypothetical ABC transporter ATP-
UniRef100_P54591

Bacillus subtilis




binding protein yhcG [Bacillus subtilis]


895
Hypothetical ABC transporter ATP-
UniRef100_P54592

Bacillus subtilis

YhcH



binding protein yhcH [Bacillus subtilis]


896
Hypothetical protein yhcI [Bacillus
UniRef100_P54593

Bacillus subtilis

YhcI




subtilis]



897
Cold shock protein cspB [Bacillus
UniRef100_P32081

Bacillus subtilis





subtilis]



898
ABC transporter, ATP-binding protein
UniRef100_Q6HP89

Bacillus

YusC



[Bacillus thuringiensis]


thuringiensis



899
MW0417 protein [Staphylococcus
UniRef100_Q8NY20

Staphylococcus

YusB




aureus]



aureus



900
ABC transporter substrate-binding
UniRef100_Q81IN6

Bacillus cereus

YhcJ



protein [Bacillus cereus]


901
Hypothetical symporter yhcL [Bacillus
UniRef100_P54596

Bacillus subtilis

YhcL




subtilis]



902
Hypothetical protein yhcM [Bacillus
UniRef100_P54597

Bacillus subtilis

YhcM




subtilis]



903
Acylamino-acid-releasing enzyme
UniRef100_Q8CXN6

Oceanobacillus

YuxL



[Oceanobacillus iheyensis]


iheyensis



904
Lipoprotein yhcN precursor [Bacillus
UniRef100_P54598

Bacillus subtilis

YhcN




subtilis]



905
Hypothetical protein yhcP [Bacillus
UniRef100_P54600

Bacillus subtilis

YhcP




subtilis]



906
Hypothetical protein yhcQ [Bacillus
UniRef100_P54601

Bacillus subtilis

YhcQ




subtilis]



907
Two-component sensor histidine kinase
UniRef100_O31661

Bacillus subtilis

KinE



[Bacillus subtilis]


908
Hypothetical protein yhcR precursor
UniRef100_P54602

Bacillus subtilis

YhcR



[Bacillus subtilis]


909
Hypothetical protein yhcS [Bacillus
UniRef100_P54603

Bacillus subtilis

YhcS




subtilis]



910
Hypothetical pseudouridine synthase
UniRef100_P54604

Bacillus subtilis

YhcT



yhcT [Bacillus subtilis]


911
Hypothetical protein yhcU [Bacillus
UniRef100_P54605

Bacillus subtilis

YhcU




subtilis]



912
Hypothetical protein yhcV [Bacillus
UniRef100_P54606

Bacillus subtilis

YhcV




subtilis]



913
Hypothetical protein yhcW [Bacillus
UniRef100_P54607

Bacillus subtilis

YhcW




subtilis]



914
Hypothetical UPF0012 protein yhcX
UniRef100_P54608

Bacillus subtilis

YhcX



[Bacillus subtilis]


915
ABC transporter [Bacillus halodurans]
UniRef100_Q9KBA3

Bacillus

YdiF






halodurans



916
Hypothetical transport protein yrhG
UniRef100_O05399

Bacillus subtilis

YrhG



[Bacillus subtilis]


917
Lin0826 protein [Listeria innocua]
UniRef100_Q92DI7

Listeria innocua

YwkB


918
Alcohol dehydrogenase [Bacillus
UniRef100_Q81CT4

Bacillus cereus

YogA




cereus]



919
Glycerol uptake operon antiterminator
UniRef100_P30300

Bacillus subtilis

GlpP



regulatory protein [Bacillus subtilis]


920
Glycerol uptake facilitator protein
UniRef100_P18156

Bacillus subtilis

GlpF



[Bacillus subtilis]


921
Glycerol kinase [Bacillus subtilis]
UniRef100_P18157

Bacillus subtilis

GlpK


922
Aerobic glycerol-3-phosphate
UniRef100_P18158

Bacillus subtilis

GlpD



dehydrogenase [Bacillus subtilis]


923
Alpha-phosphoglucomutase [Bacillus
UniRef100_Q68VA2

Bacillus subtilis

YhxB




subtilis subsp. subtilis]


subsp. subtilis


924
Hypothetical conserved protein
UniRef100_Q8ELS7

Oceanobacillus

YcgB



[Oceanobacillus iheyensis]


iheyensis



925
Transcriptional regulator
UniRef100_Q8ELS8

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


926



YhcY


927
Hypothetical protein yhcZ [Bacillus
UniRef100_O07528

Bacillus subtilis

YhcZ




subtilis]



928
Hypothetical protein yhdA [Bacillus
UniRef100_O07529

Bacillus subtilis

YhdA




subtilis]



929


930


931
Hypothetical UPF0074 protein yhdE
UniRef100_O07573

Bacillus subtilis

YhdE



[Bacillus subtilis]


932
Flavohemoprotein [Bacillus halodurans]
UniRef100_Q9RC40

Bacillus

Hmp






halodurans



933
Stage V sporulation protein R [Bacillus
UniRef100_P37875

Bacillus subtilis

SpoVR




subtilis]



934
Probable endopeptidase IytE precursor
UniRef100_P54421

Bacillus subtilis

LytE



[Bacillus subtilis]


935



CitR


936



CitA


937
Glucose dehydrogenase-B [Bacillus
UniRef100_Q9Z9R3

Bacillus halodurans





halodurans]



938
Hypothetical oxidoreductase yhdF
UniRef100_O07575

Bacillus subtilis

YhdF



[Bacillus subtilis]


939
Hypothetical protein yhdH [Bacillus
UniRef100_O07577

Bacillus subtilis

YhdH




subtilis]



940
2,4-diaminobutyrate decarboxylase
UniRef100_Q9KFB9

Bacillus halodurans




[Bacillus halodurans]


941
YdeE protein [Bacillus subtilis]
UniRef100_P96662

Bacillus subtilis

YdeE


942
YdeL protein [Bacillus subtilis]
UniRef100_P96669

Bacillus subtilis

YdeL


943
BH1582 protein [Bacillus halodurans]
UniRef100_Q9KCI9

Bacillus

YhdJ






halodurans



944
YhdK [Bacillus subtilis subsp. spizizenii]
UniRef100_Q7X2K9

Bacillus subtilis subsp.







spizizenii



945
YhdL [Bacillus subtilis subsp. spizizenii]
UniRef100_Q7X2L0

Bacillus subtilis

YhdL





subsp. spizizenii


946
Hypothetical protein yhdM (RNA
UniRef100_O07582

Bacillus subtilis

SigM



polymerase ECF (Extracytoplasmic



function)-type sigma factor) [Bacillus




subtilis]



947



YrkC


948
Hypothetical protein yhdO [Bacillus
UniRef100_O07584

Bacillus subtilis

YhdO




subtilis]



949


950
Acyl-CoA thioesterase 1 [Clostridium
UniRef100_Q97DR5

Clostridium acetobutylicum





acetobutylicum]



951
UPI00003CB259 UniRef100 entry
UniRef100_UPI00003CB259

YvkB


952



YhdP


953
HTH-type transcriptional regulator cueR
UniRef100_O07586

Bacillus subtilis

YhdQ



[Bacillus subtilis]


954



YhdT


955


956
BH3511 protein [Bacillus halodurans]
UniRef100_Q9K762

Bacillus halodurans



957
Sporulation specific N-acetylmuramoyl-
UniRef100_Q8CX69

Oceanobacillus

CwlC



L-alanine amidase [Oceanobacillus


iheyensis





iheyensis]



958
Protein crcB homolog 1 [Bacillus
UniRef100_O07590

Bacillus subtilis

YhdU




subtilis]



959
Protein crcB homolog 2 [Bacillus
UniRef100_O07591

Bacillus subtilis

YhdV




subtilis]



960



YhdW


961


962
Hypothetical UPF0003 protein yhdY
UniRef100_O07594

Bacillus subtilis

YhdY



[Bacillus subtilis]


963
NAD-dependent deacetylase [Bacillus
UniRef100_O07595

Bacillus subtilis

YhdZ




subtilis]



964


965
Hypothetical protein yheN [Bacillus
UniRef100_O07596

Bacillus subtilis

YheN




subtilis]



966



Dat


967
Na(+)/H(+) antiporter [Bacillus subtilis]
UniRef100_O07553

Bacillus subtilis

NhaC


968
Hypothetical protein yoxA [Bacillus
UniRef100_P39840

Bacillus subtilis

YoxA




subtilis]



969
Hypothetical protein ydhH [Bacillus
UniRef100_O05500

Bacillus subtilis

YdhH




subtilis]



970
Hypothetical protein [Bacillus cereus
UniRef100_Q63EB4

Bacillus cereus ZK




ZK]


971
Hypothetical protein [Bacillus cereus]
UniRef100_Q81GF4

Bacillus cereus



972
Stress response protein nhaX [Bacillus
UniRef100_O07552

Bacillus subtilis

NhaX




subtilis]



973
Hypothetical protein yheI [Bacillus
UniRef100_O07550

Bacillus subtilis

YheI




subtilis]



974
Hypothetical protein yheH [Bacillus
UniRef100_O07549

Bacillus subtilis

YheH




subtilis]



975
Hypothetical protein yheG [Bacillus
UniRef100_O07548

Bacillus subtilis

YheG




subtilis]



976
Small, acid-soluble spore protein B
UniRef100_P04832

Bacillus subtilis




[Bacillus subtilis]


977
BH1139 protein [Bacillus halodurans]
UniRef100_Q9KDS2

Bacillus halodurans



978
Sugar ABC transporter ATP-binding
UniRef100_Q8CUH3

Oceanobacillus

MsmX



protein [Oceanobacillus iheyensis]


iheyensis



979
Fis-type helix-turn-helix domain protein
UniRef100_Q73A84

Bacillus cereus

YxkF



[Bacillus cereus]


980
Hypothetical protein yheE [Bacillus
UniRef100_O07546

Bacillus subtilis





subtilis]



981
Hypothetical protein yheD [Bacillus
UniRef100_O07545

Bacillus subtilis

YheD




subtilis]



982
Hypothetical protein yheC [Bacillus
UniRef100_O07544

Bacillus subtilis

YheC




subtilis]



983
Hypothetical protein yheB [Bacillus
UniRef100_O07543

Bacillus subtilis

YheB




subtilis]



984
Hypothetical protein yheA [Bacillus
UniRef100_O07542

Bacillus subtilis

YheA




subtilis]



985
Stress response protein yhaX [Bacillus
UniRef100_O07539

Bacillus subtilis

YhaX




subtilis]



986



HemZ


987
YhaR protein [Bacillus subtilis]
UniRef100_O07533

Bacillus subtilis

YhaR


988
Response regulator aspartate
UniRef100_P96649

Bacillus subtilis

RapI



phosphatase I [Bacillus subtilis]


989


990
Hypothetical protein yhaQ [Bacillus
UniRef100_O07524

Bacillus subtilis

YhaQ




subtilis]



991
Hypothetical protein yhaP [Bacillus
UniRef100_O07523

Bacillus subtilis

YhaP




subtilis]



992



YhaO


993
Hypothetical protein yhaN [Bacillus
UniRef100_O08455

Bacillus subtilis

YhaN




subtilis]



994



YhaM


995
Hypothetical protein yhaL [Bacillus
UniRef100_O07520

Bacillus subtilis





subtilis]



996
Foldase protein prsA precursor
UniRef100_P24327

Bacillus subtilis

PrsA



[Bacillus subtilis]


997


998
Hypothetical protein yhaK [Bacillus
UniRef100_O07519

Bacillus subtilis





subtilis]



999
Hypothetical protein yhaI [Bacillus
UniRef100_O07517

Bacillus subtilis

YhaI




subtilis]



1000
Protease production regulatory protein
UniRef100_P11065

Bacillus subtilis

Hpr



hpr [Bacillus subtilis]


1001
Hypothetical protein yhaH [Bacillus
UniRef100_O07516

Bacillus subtilis

YhaH




subtilis]



1002
Probable tryptophan transport protein
UniRef100_O07515

Bacillus subtilis

YhaG



[Bacillus subtilis]


1003
Phosphoserine aminotransferase
UniRef100_P80862

Bacillus subtilis

SerC



[Bacillus subtilis]


1004
Hit protein [Bacillus subtilis]
UniRef100_O07513

Bacillus subtilis

Hit


1005


1006
ABC-type transporter ATP-binding
UniRef100_P55339

Bacillus subtilis

EcsA



protein ecsA [Bacillus subtilis]


1007
Protein ecsB [Bacillus subtilis]
UniRef100_P55340

Bacillus subtilis

EcsB


1008
Protein ecsC [Bacillus subtilis]
UniRef100_P55341

Bacillus subtilis

EcsC


1009
YhaA protein [Bacillus subtilis]
UniRef100_O07598

Bacillus subtilis

YhaA


1010
Hypothetical protein yhfA [Bacillus
UniRef100_O07599

Bacillus subtilis

YhfA




subtilis]



1011
Hypothetical protein yhgC [Bacillus
UniRef100_P38049

Bacillus subtilis

YhgC




subtilis]



1012
Penicillin-binding protein 1F [Bacillus
UniRef100_P38050

Bacillus subtilis

PbpF




subtilis]



1013
Uroporphyrinogen decarboxylase
UniRef100_P32395

Bacillus subtilis

HemE



[Bacillus subtilis]


1014
Ferrochelatase [Bacillus subtilis]
UniRef100_P32396

Bacillus subtilis

HemH


1015
Protoporphyrinogen oxidase [Bacillus
UniRef100_P32397

Bacillus subtilis

HemY




subtilis]



1016



YhgD


1017
Hypothetical protein yhgE [Bacillus
UniRef100_P32399

Bacillus subtilis

YhgE




subtilis]



1018
3-oxoacyl-[acyl-carrier-protein]
UniRef100_O07600
acyl-carrier-
FabHB



synthase III protein 2 [Bacillus subtilis]

protein


1019
Hypothetical protein yhfE [Bacillus
UniRef100_O07603

Bacillus subtilis

YhfE




subtilis]



1020


1021
Hypothetical protein yhfG [Bacillus
UniRef100_O07605

Bacillus subtilis

GltT




subtilis]



1022
Hypothetical protein yhfI [Bacillus
UniRef100_O07607

Bacillus subtilis

YhfI




subtilis]



1023
Hypothetical protein yhfJ [Bacillus
UniRef100_O07608

Bacillus subtilis

YhfJ




subtilis]



1024
Hypothetical protein yhfL [Bacillus
UniRef100_O07610

Bacillus subtilis

YhfL




subtilis]



1025
Hypothetical protein yhfM precursor
UniRef100_O07611

Bacillus subtilis

YhfM



[Bacillus subtilis]


1026
BH2909 protein [Bacillus halodurans]
UniRef100_Q9K8U3

Bacillus halodurans



1027
Branched-chain amino acid transporter
UniRef100_Q9K8U2

Bacillus

AzlC



[Bacillus halodurans]


halodurans



1028
BH2911 protein [Bacillus halodurans]
UniRef100_Q9K8U1

Bacillus halodurans



1029
Putative metalloprotease yhfN [Bacillus
UniRef100_P40769

Bacillus subtilis

YhfN




subtilis]



1030



AprE


1031
Transporter, drug/metabolite exporter
UniRef100_Q63D40

Bacillus cereus

YdeD



family [Bacillus cereus ZK]

ZK


1032
Hypothetical protein yhfQ [Bacillus
UniRef100_O07616

Bacillus subtilis

YhfQ




subtilis]



1033
YfmD protein [Bacillus subtilis]
UniRef100_O34933

Bacillus subtilis

YfmD


1034
YfmE protein [Bacillus subtilis]
UniRef100_O34832

Bacillus subtilis

YfmE


1035
Hypothetical protein yhfR [Bacillus
UniRef100_O07617

Bacillus subtilis

YhfR




subtilis]



1036
Heme-based aerotactic transducer
UniRef100_O07621

Bacillus subtilis

HemAT



hemAT [Bacillus subtilis]


1037
Rieske 2Fe—2S iron-sulfur protein,
UniRef100_Q73E94

Bacillus cereus

YhfW



putative [Bacillus cereus]


1038



YhxC


1039
Hypothetical protein yhzC [Bacillus
UniRef100_O31594

Bacillus subtilis





subtilis]



1040



ComK


1041



YhjD


1042



YhjE


1043
Signal peptidase I V [Bacillus subtilis]
UniRef100_O07560

Bacillus subtilis

SipV


1044
Minor extracellular protease epr
UniRef100_P16396

Bacillus subtilis

Epr



precursor [Bacillus subtilis]


1045
Putative permease [Klebsiella
UniRef100_Q765R6

Klebsiella

YybO




pneumoniae]



pneumoniae



1046
Hypothetical protein [Enterococcus
UniRef100_Q82ZQ4

Enterococcus

PucR




faecalis]



faecalis



1047
Putative allantoinase [Staphylococcus
UniRef100_Q9EV52

Staphylococcus

PucH




xylosus]



xylosus



1048
Peptidase, M20/M25/M40 family
UniRef100_Q82ZQ2

Enterococcus

YurH



[Enterococcus faecalis]


faecalis



1049
Hypothetical protein STY0574
UniRef100_Q8XFX7

Salmonella typhi




[Salmonella typhi]


1050
Ureidoglycolate dehydrogenase
UniRef100_Q838P9

Enterococcus

YjmC



[Enterococcus faecalis]


faecalis



1051
Ureidoglycolate dehydrogenase
UniRef100_Q838P9

Enterococcus

YjmC



[Enterococcus faecalis]


faecalis



1052
Hypothetical protein [Enterococcus
UniRef100_Q838Q3

Enterococcus

SucD




faecalis]



faecalis



1053
Hypothetical protein [Enterococcus
UniRef100_Q838Q3

Enterococcus faecalis





faecalis]



1054


1055
Carbamate kinase [Clostridium tetani]
UniRef100_Q890W1

Clostridium tetani



1056
Major facilitator family transporter
UniRef100_Q838Q1

Enterococcus

YcbE



[Enterococcus faecalis]


faecalis



1057
Hypothetical protein yjfA precursor
UniRef100_O34554

Bacillus subtilis




[Bacillus subtilis]


1058
Response regulator aspartate
UniRef100_O32294

Bacillus subtilis

RapG



phosphatase G [Bacillus subtilis]


1059


1060


1061


1062
Lacl-family transcription regulator
UniRef100_O34829

Bacillus subtilis

MsmR



[Bacillus subtilis]


1063
Multiple sugar-binding protein [Bacillus
UniRef100_O34335

Bacillus subtilis

MsmE




subtilis]



1064
Sugar transporter [Bacillus subtilis]
UniRef100_O34706

Bacillus subtilis

AmyD


1065
Sugar transporter [Bacillus subtilis]
UniRef100_O34518

Bacillus subtilis

AmyC


1066
Alpha-galactosidase [Bacillus subtilis]
UniRef100_O34645

Bacillus subtilis

MelA


1067
Hypothetical protein yhjN [Bacillus
UniRef100_O07568

Bacillus subtilis

YhjN




subtilis]



1068
Spore coat-associated protein JA
UniRef100_Q63FK5

Bacillus cereus ZK




[Bacillus cereus ZK]


1069
CotJB protein [Bacillus subtilis]
UniRef100_Q45537

Bacillus subtilis



1070
CotJC protein [Bacillus subtilis]
UniRef100_Q45538

Bacillus subtilis

CotJC


1071
Long-chain fatty-acid-CoA ligase
UniRef100_Q9KDT0

Bacillus

YngI



[Bacillus halodurans]


halodurans



1072
Hypothetical protein yhjO [Bacillus
UniRef100_O07569

Bacillus subtilis

YhjO




subtilis]



1073
Hypothetical protein [Bacillus cereus
UniRef100_Q63FZ3

Bacillus cereus

LytB



ZK]

ZK


1074
Sensor histidine kinase [Bacillus
UniRef100_Q6HNG3

Bacillus

PhoR




thuringiensis]



thuringiensis



1075
Two-component response regulator
UniRef100_Q81I36

Bacillus cereus

YclJ



[Bacillus cereus]


1076



YhjR


1077
Putative molybdate binding protein,
UniRef100_O32208

Bacillus subtilis

YvgL



YvgL [Bacillus subtilis]


1078
Putative molybdate transport protein,
UniRef100_O32209

Bacillus subtilis

YvgM



YvgM [Bacillus subtilis]


1079
ATP-dependent nuclease subunit B
UniRef100_P23477

Bacillus subtilis

AddB



[Bacillus subtilis]


1080
ATP-dependent nuclease subunit A
UniRef100_P23478

Bacillus subtilis

AddA



[Bacillus subtilis]


1081
Exonuclease sbcD homolog [Bacillus
UniRef100_P23479

Bacillus subtilis

SbcD




subtilis]



1082
Nuclease sbcCD subunit C [Bacillus
UniRef100_O06714

Bacillus subtilis

YirY




subtilis]



1083
Probable spore germination protein
UniRef100_O06716

Bacillus subtilis




gerPF [Bacillus subtilis]


1084
Probable spore germination protein
UniRef100_O06717

Bacillus subtilis

GerPE



gerPE [Bacillus subtilis]


1085
Probable spore germination protein
UniRef100_O06718

Bacillus subtilis




gerPD [Bacillus subtilis]


1086
Probable spore germination protein
UniRef100_O06719

Bacillus subtilis

GerPC



gerPC [Bacillus subtilis]


1087
Probable spore germination protein
UniRef100_O06720

Bacillus subtilis




gerPB [Bacillus subtilis]


1088
Probable spore germination protein
UniRef100_O06721

Bacillus subtilis




gerPA [Bacillus subtilis]


1089
Hypothetical protein yitR [Bacillus
UniRef100_O06753

Bacillus subtilis





subtilis]



1090


1091
Spore coat protein H [Bacillus cereus]
UniRef100_Q81EE9

Bacillus cereus

CotH


1092



CotG


1093
YisK [Bacillus subtilis]
UniRef100_O06724

Bacillus subtilis

YisK


1094
Hypothetical protein yloQ [Bacillus
UniRef100_Q63CF2

Bacillus cereus

YloQ




cereus ZK]


ZK


1095
YisL [Bacillus subtilis]
UniRef100_O06725

Bacillus subtilis

YisL


1096
Hypothetical protein yisN [Bacillus
UniRef100_O06727

Bacillus subtilis

YisN




subtilis]



1097
Asparagine synthetase [glutamine-
UniRef100_O05272
glutamine-
AsnO



hydrolyzing] 3 [Bacillus subtilis]

hydrolyzing


1098



NrgA


1099
Nitrogen regulatory PII protein
UniRef100_Q8ERT8

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


1100
YisQ [Bacillus subtilis]
UniRef100_O07940

Bacillus subtilis

YisQ


1101
Putative HTH-type transcriptional
UniRef100_P40331

Bacillus subtilis

YisR



regulator yisR [Bacillus subtilis]


1102
Acetyltransferase, GNAT family
UniRef100_Q63C80

Bacillus cereus

YokL



[Bacillus cereus ZK]

ZK


1103
HTH-type transcriptional regulator degA
UniRef100_P37947

Bacillus subtilis

DegA



[Bacillus subtilis]


1104
Hypothetical oxidoreductase yisS
UniRef100_P40332

Bacillus subtilis

YisS



[Bacillus subtilis]


1105
YisV protein [Bacillus subtilis]
UniRef100_Q796Q6

Bacillus subtilis

YisV


1106
Diaminobutyrate--pyruvate
UniRef100_Q9K9M1

Bacillus

GabT



transaminase [Bacillus halodurans]


halodurans



1107
L-2,4-diaminobutyrate decarboxylase
UniRef100_Q8YZR2

Anabaena sp.




[Anabaena sp.]


1108
AII0394 protein [Anabaena sp.]
UniRef100_Q8YZR3

Anabaena sp.



1109
BH2621 protein [Bacillus halodurans]
UniRef100_Q9K9M4

Bacillus halodurans



1110
BH2620 protein [Bacillus halodurans]
UniRef100_Q9K9M5

Bacillus halodurans



1111
BH2618 protein [Bacillus halodurans]
UniRef100_Q9K9M7

Bacillus halodurans



1112
YitI protein [Bacillus subtilis]
UniRef100_O06744

Bacillus subtilis

YitI


1113
Glr2355 protein [Gloeobacter violaceus]
UniRef100_Q7N129

Gloeobacter

YcdF






violaceus



1114
BH0411 protein [Bacillus halodurans]
UniRef100_Q9KFR6

Bacillus

YobV






halodurans



1115
5-methyltetrahydrofolate S-
UniRef100_Q9KCE1

Bacillus

YitJ



homocysteine methyltransferase


halodurans




[Bacillus halodurans]


1116
YitJ [Bacillus subtilis]
UniRef100_O06745

Bacillus subtilis

YitJ


1117
Hypothetical UPF0234 protein yitk
UniRef100_O06746

Bacillus subtilis

YitK



[Bacillus subtilis]


1118
YitL protein [Bacillus subtilis]
UniRef100_O06747

Bacillus subtilis

YitL


1119


1120


1121
Hypothetical UPF0230 protein yitS
UniRef100_P70945

Bacillus subtilis

YitS



[Bacillus subtilis]


1122
Hypothetical protein yitT [Bacillus
UniRef100_P39803

Bacillus subtilis

YitT




subtilis]



1123
Intracellular proteinase inhibitor
UniRef100_P39804

Bacillus subtilis

Ipi



[Bacillus subtilis]


1124
GMP reductase [Bacillus subtilis]
UniRef100_O05269

Bacillus subtilis

GuaC


1125


1126


1127
Putative orf protein [Bacillus subtilis]
UniRef100_P70947

Bacillus subtilis

YitU


1128
Putative orf protein [Bacillus subtilis]
UniRef100_P70948

Bacillus subtilis

YitV


1129



YitW


1130
N-acetyl-gamma-glutamyl-phosphate
UniRef100_P23715

Bacillus subtilis

ArgC



reductase [Bacillus subtilis]


1131
Arginine biosynthesis bifunctional
UniRef100_Q9ZJ14
Includes:
ArgJ



protein argJ [Includes: Glutamate N-

Glutamate N-



acetyltransferase (EC 2.3.1.35)

acetyltransferase



(Ornithine acetyltransferase) (Ornithine

(EC 2.3.1.35)



transacetylase) (OATase); Amino-acid

(Ornithine



acetyltransferase (EC 2.3.1.1) (N-

acetyltransferase)



acetylglutamate synthase) (AGS)]

(Ornithine



[Contai

transacetylase)





(OATase);





Amino-acid





acetyltransferase





(EC 2.3.1.1) (N-





acetylglutamate





synthase) (AGS)


1132
Acetylglutamate kinase [Bacillus
UniRef100_P36840

Bacillus subtilis

ArgB




subtilis]



1133
Acetylornithine aminotransferase
UniRef100_P36839

Bacillus subtilis

ArgD



[Bacillus subtilis]


1134
Carbamoyl-phosphate synthase,
UniRef100_P36838

Bacillus subtilis

CarA



arginine-specific, small chain [Bacillus




subtilis]



1135
Carbamoyl-phosphate synthase,
UniRef100_P18185

Bacillus subtilis

CarB



arginine-specific, large chain [Bacillus




subtilis]



1136
Ornithine carbamoyltransferase
UniRef100_P18186

Bacillus subtilis

ArgF



[Bacillus subtilis]


1137
Undecaprenyl-diphosphatase 1
UniRef100_Q81HV4

Bacillus cereus

YubB



[Bacillus cereus]


1138
YjzC protein [Bacillus subtilis]
UniRef100_O34585

Bacillus subtilis



1139


1140
Hypothetical protein yjaU [Bacillus
UniRef100_O35001

Bacillus subtilis

YjaU




subtilis]



1141
ArgF and med genes, partial and
UniRef100_O32435

Bacillus subtilis

YjaV



complete cds [Bacillus subtilis]


1142
Transcriptional activator protein med
UniRef100_O32436

Bacillus subtilis

Med



precursor [Bacillus subtilis]


1143
ComG operon repressor [Bacillus
UniRef100_O32437

Bacillus subtilis





subtilis]



1144
Hypothetical protein yjzB [Bacillus
UniRef100_O34891

Bacillus subtilis





subtilis]



1145
3-oxoacyl-[acyl-carrier-protein]
UniRef100_O34746
acyl-carrier-
FabHA



synthase III protein 1 [Bacillus subtilis]

protein


1146
Beta-ketoacyl-acyl carrier protein
UniRef100_O34340

Bacillus subtilis

FabF



synthase II [Bacillus subtilis]


1147
YjaZ protein [Bacillus subtilis]
UniRef100_O31596

Bacillus subtilis

YjaZ


1148
Oligopeptide transport ATP-binding
UniRef100_P42064

Bacillus subtilis

AppD



protein appD [Bacillus subtilis]


1149
Oligopeptide transport ATP-binding
UniRef100_P42065

Bacillus subtilis

AppF



protein appF [Bacillus subtilis]


1150
Oligopeptide-binding protein appA
UniRef100_P42061

Bacillus subtilis

AppA



precursor [Bacillus subtilis]


1151
Oligopeptide transport system
UniRef100_P42062

Bacillus subtilis

AppB



permease protein appB [Bacillus




subtilis]



1152
Oligopeptide transport system
UniRef100_P42063

Bacillus subtilis

AppC



permease protein appC [Bacillus




subtilis]



1153
Permease, putative [Bacillus cereus]
UniRef100_Q734Y3

Bacillus cereus

YvqJ


1154



YjbA


1155
Tryptophanyl-tRNA synthetase [Bacillus
UniRef100_P21656

Bacillus subtilis

TrpS




subtilis]



1156
Oligopeptide-binding protein oppA
UniRef100_P24141

Bacillus subtilis

OppA



precursor [Bacillus subtilis]


1157
Oligopeptide transport system
UniRef100_P24138

Bacillus subtilis

OppB



permease protein oppB [Bacillus




subtilis]



1158
Oligopeptide transport system
UniRef100_P24139

Bacillus subtilis

OppC



permease protein oppC [Bacillus




subtilis]



1159
Oligopeptide transport ATP-binding
UniRef100_P24136

Bacillus subtilis

OppD



protein oppD [Bacillus subtilis]


1160
Oligopeptide transport ATP-binding
UniRef100_P24137

Bacillus subtilis

OppF



protein oppF [Bacillus subtilis]


1161
YjbC protein [Bacillus subtilis]
UniRef100_O31601

Bacillus subtilis

YjbC


1162
Regulatory protein spx [Bacillus subtilis]
UniRef100_O31602

Bacillus subtilis

YjbD


1163
YjbE protein [Bacillus subtilis]
UniRef100_O31603

Bacillus subtilis

YjbE


1164
Adapter protein mecA 1 [Bacillus
UniRef100_P37958

Bacillus subtilis

MecA




subtilis]



1165
Hypothetical conserved protein
UniRef100_Q8ELH8

Oceanobacillus

YflP



[Oceanobacillus iheyensis]


iheyensis



1166
Hypothetical protein OB3248
UniRef100_Q8ELH9

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


1167
Hypothetical conserved protein
UniRef100_Q8ELI0

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


1168
Response regulator of citrate/malate
UniRef100_Q7ML23

Vibrio vulnificus

CitT



metabolism [Vibrio vulnificus]


1169
Sensor protein citS [Bacillus
UniRef100_Q9RC53

Bacillus

YufL




halodurans]



halodurans



1170
YjbF protein [Bacillus subtilis]
UniRef100_O31604

Bacillus subtilis

YjbF


1171



YjbG


1172


1173
YjbH protein [Bacillus subtilis]
UniRef100_O31606

Bacillus subtilis

YjbH


1174
YjbI protein [Bacillus subtilis]
UniRef100_O31607

Bacillus subtilis

YjbI


1175
YjbJ protein [Bacillus subtilis]
UniRef100_O31608

Bacillus subtilis

YjbJ


1176
YjbK protein [Bacillus subtilis]
UniRef100_O31609

Bacillus subtilis

YjbK


1177
YjbL protein [Bacillus subtilis]
UniRef100_O31610

Bacillus subtilis

YjbL


1178
YjbM protein [Bacillus subtilis]
UniRef100_O31611

Bacillus subtilis

YjbM


1179



YjbN


1180
Hypothetical pseudouridine synthase
UniRef100_O31613

Bacillus subtilis

YjbO



yjbO [Bacillus subtilis]


1181
YjbP protein [Bacillus subtilis]
UniRef100_O31614

Bacillus subtilis

YjbP


1182
YjbQ protein [Bacillus subtilis]
UniRef100_O31615

Bacillus subtilis

YjbQ


1183
Transcriptional activator tenA [Bacillus
UniRef100_P25052

Bacillus subtilis

TenA




subtilis]



1184
Regulatory protein tenI [Bacillus
UniRef100_P25053

Bacillus subtilis

TenI




subtilis]



1185
Glycine oxidase [Bacillus subtilis]
UniRef100_O31616

Bacillus subtilis

GoxB


1186
ThiS protein [Bacillus subtilis]
UniRef100_O31617

Bacillus subtilis



1187
Thiazole biosynthesis protein thiG
UniRef100_O31618

Bacillus subtilis

ThiG



[Bacillus subtilis]


1188
ThiF protein [Bacillus subtilis]
UniRef100_O31619

Bacillus subtilis

ThiF


1189
YjbV protein [Bacillus subtilis]
UniRef100_O31620

Bacillus subtilis

YjbV


1190
Enoyl-[acyl-carrier-protein] reductase
UniRef100_P54616
acyl-carrier-
FabI



[NADH] [Bacillus subtilis]

protein


1191
YjbX protein [Bacillus subtilis]
UniRef100_O31622

Bacillus subtilis

YjbX


1192
Spore coat protein Z [Bacillus subtilis]
UniRef100_Q08312

Bacillus subtilis

CotZ


1193
Spore coat protein Y [Bacillus subtilis]
UniRef100_Q08311

Bacillus subtilis

CotY


1194
Spore coat protein X [Bacillus subtilis]
UniRef100_Q08313

Bacillus subtilis

CotX


1195
Spore coat protein W [Bacillus subtilis]
UniRef100_Q08310

Bacillus subtilis

CotW


1196
Spore coat protein V [Bacillus subtilis]
UniRef100_Q08309

Bacillus subtilis

CotV


1197
YjcA protein [Bacillus subtilis]
UniRef100_O31623

Bacillus subtilis

YjcA


1198


1199


1200
YjcC protein [Bacillus subtilis]
UniRef100_O31625

Bacillus subtilis



1201



YjcD


1202



YngC


1203



GalE


1204
YngB protein [Bacillus subtilis]
UniRef100_O31822

Bacillus subtilis

YngB


1205
YngA protein [Bacillus
UniRef100_Q70JY6

Bacillus

YngA




amyloliquefaciens]



amyloliquefaciens



1206
YjcF protein [Bacillus subtilis]
UniRef100_O31628

Bacillus subtilis

YjcF


1207
YjcG protein [Bacillus subtilis]
UniRef100_O31629

Bacillus subtilis

YjcG


1208
YjcH protein [Bacillus subtilis]
UniRef100_O31630

Bacillus subtilis

YjcH


1209
Hypothetical protein [Bacillus cereus]
UniRef100_Q739H9

Bacillus cereus



1210
BH1889 protein [Bacillus halodurans]
UniRef100_Q9KBN6

Bacillus

YobV






halodurans



1211
YjcI protein [Bacillus subtilis]
UniRef100_O31631

Bacillus subtilis

YjcI


1212
YjcJ protein [Bacillus subtilis]
UniRef100_O31632

Bacillus subtilis

YjcJ


1213
YjcL protein [Bacillus subtilis]
UniRef100_O31634

Bacillus subtilis

YjcL


1214
Transcriptional regulator, MarR/EmrR
UniRef100_Q97DR6

Clostridium acetobutylicum




family [Clostridium acetobutylicum]


1215
Penicillin-binding protein 4* [Bacillus
UniRef100_P32959

Bacillus subtilis

PbpE




subtilis]



1216



AbnA


1217


1218
Maltose transacetylase [Bacillus
UniRef100_Q75TH6

Bacillus

Maa




stearothermophilus]



stearothermophilus



1219


1220
Putative HTH-type transcriptional
UniRef100_P39647

Bacillus subtilis

YwfK



regulator ywfK [Bacillus subtilis]


1221
Sulfite reductase [Bacillus halodurans]
UniRef100_Q9KF76

Bacillus

YvgR






halodurans



1222
Sulfite reductase [Bacillus halodurans]
UniRef100_Q9KF75

Bacillus

YvgQ






halodurans



1223
Putative HTH-type transcriptional
UniRef100_O34701

Bacillus subtilis

YoaU



regulator yoaU [Bacillus subtilis]


1224
Hypothetical transport protein yoaV
UniRef100_O34416

Bacillus subtilis

YoaV



[Bacillus subtilis]


1225
Hypothetical protein VPA0302 [Vibrio
UniRef100_Q87JF1

Vibrio

YyaH




parahaemolyticus]



parahaemolyticus



1226
Hypothetical protein yoeB precursor
UniRef100_O34841

Bacillus subtilis

YoeB



[Bacillus subtilis]


1227



YocH


1228
Permease, general substrate
UniRef100_Q6HMC3

Bacillus

LmrB



transporter [Bacillus thuringiensis]


thuringiensis



1229
Putative HTH-type transcriptional
UniRef100_P42105

Bacillus subtilis

YxaF



regulator yxaF [Bacillus subtilis]


1230


1231



YmzD


1232



YeeF


1233



YjqB


1234
Phage-like element PBSX protein xkdA
UniRef100_P39780

Bacillus subtilis

XkdA



[Bacillus subtilis]


1235
HTH-type transcriptional regulator xre
UniRef100_P23789

Bacillus subtilis

Xre



[Bacillus subtilis]


1236


1237


1238
Phage-like element PBSX protein xkdB
UniRef100_P39781

Bacillus subtilis

XkdB



[Bacillus subtilis]


1239
Phage-like element PBSX protein xkdC
UniRef100_P39782

Bacillus subtilis

XkdC



[Bacillus subtilis]


1240
Phage-like element PBSX protein xkdD
UniRef100_P39783

Bacillus subtilis

XkdD



[Bacillus subtilis]


1241
Phage-like element PBSX protein xtrA
UniRef100_P54344

Bacillus subtilis




[Bacillus subtilis]


1242
Positive control factor [Bacillus subtilis]
UniRef100_P39784

Bacillus subtilis

Xpf


1243
PBSX phage terminase small subunit
UniRef100_P39785

Bacillus subtilis

XtmA



[Bacillus subtilis]


1244
PBSX phage terminase large subunit
UniRef100_P39786

Bacillus subtilis

XtmB



[Bacillus subtilis]


1245
Phage-like element PBSX protein xkdE
UniRef100_P54325

Bacillus subtilis

XkdE



[Bacillus subtilis]


1246
Phage-like element PBSX protein xkdF
UniRef100_P54326

Bacillus subtilis

XkdF



[Bacillus subtilis]


1247
Phage-like element PBSX protein xkdG
UniRef100_P54327

Bacillus subtilis

XkdG



[Bacillus subtilis]


1248
Hypothetical protein yqbG [Bacillus
UniRef100_P45923

Bacillus subtilis

YqbG




subtilis]



1249
Hypothetical protein yqbH [Bacillus
UniRef100_P45924

Bacillus subtilis

YqbH




subtilis]



1250
Phage-like element PBSX protein xkdI
UniRef100_P54329

Bacillus subtilis

XkdI



[Bacillus subtilis]


1251
Phage-like element PBSX protein xkdJ
UniRef100_P54330

Bacillus subtilis

XkdJ



[Bacillus subtilis]


1252
Lin1277 protein [Listeria innocua]
UniRef100_Q92CB2

Listeria innocua



1253
Phage-like element PBSX protein xkdK
UniRef100_P54331

Bacillus subtilis

XkdK



[Bacillus subtilis]


1254
Phage-like element PBSX protein xkdM
UniRef100_P54332

Bacillus subtilis

XkdM



[Bacillus subtilis]


1255
Phage-like element PBSX protein xkdN
UniRef100_P54333

Bacillus subtilis

XkdN



[Bacillus subtilis]


1256
Phage-like element PBSX protein xkdO
UniRef100_P54334

Bacillus subtilis

XkdO



[Bacillus subtilis]


1257
Phage-like element PBSX protein xkdP
UniRef100_P54335

Bacillus subtilis

YqbP



[Bacillus subtilis]


1258
Hypothetical protein yqbQ [Bacillus
UniRef100_P45950

Bacillus subtilis

YqbQ




subtilis]



1259
Hypothetical protein yqbR [Bacillus
UniRef100_P45933

Bacillus subtilis

YqbR




subtilis]



1260
Phage-like element PBSX protein xkdS
UniRef100_P54338

Bacillus subtilis

XkdS



[Bacillus subtilis]


1261
Hypothetical protein yqbT [Bacillus
UniRef100_P45935

Bacillus subtilis

YqbT




subtilis]



1262
Phage-like element PBSX protein xkdU
UniRef100_P54340

Bacillus subtilis

XkdU



[Bacillus subtilis]


1263


1264



XkdV


1265



YomR


1266


1267


1268



BlyA


1269
Regulatory protein [Bacillus
UniRef100_Q9ZFL9

Bacillus

YdhC




stearothermophilus]



stearothermophilus



1270
Zinc-containing alcohol dehydrogenase
UniRef100_O35045

Bacillus subtilis

YjmD



[Bacillus subtilis]


1271
Mannonate dehydratase 1 [Bacillus
UniRef100_Q9KDZ8

Bacillus

UxuA




halodurans]



halodurans



1272
D-mannonate oxidoreductase [Bacillus
UniRef100_Q9KDZ4

Bacillus

YjmF




halodurans]



halodurans



1273
UPI00002F2634 UniRef100 entry
UniRef100_UPI00002F2634

YjmD


1274
Hexuronate transporter [Bacillus
UniRef100_O34456

Bacillus subtilis

ExuT




subtilis]



1275
Stage II sporulation protein SB [Bacillus
UniRef100_O34800

Bacillus subtilis





subtilis]



1276
Stage II sporulation protein SA [Bacillus
UniRef100_O34853

Bacillus subtilis

SpoIISA




subtilis]



1277
UPI00003CC121 UniRef100 entry
UniRef100_UPI00003CC121

Pit


1278
Hypothetical UPF0111 protein ykaA
UniRef100_O34454

Bacillus subtilis

YkaA



[Bacillus subtilis]


1279



Ggt


1280
YesL protein [Bacillus subtilis]
UniRef100_O31515

Bacillus subtilis

YesL


1281
YesM protein [Bacillus subtilis]
UniRef100_O31516

Bacillus subtilis

YesM


1282
YesN protein [Bacillus subtilis]
UniRef100_O31517

Bacillus subtilis

YesN


1283
YesO protein [Bacillus subtilis]
UniRef100_O31518

Bacillus subtilis

YesO


1284
Probable ABC transporter permease
UniRef100_O31519

Bacillus subtilis

YesP



protein yesP [Bacillus subtilis]


1285
Probable ABC transporter permease
UniRef100_O31520

Bacillus subtilis

YesQ



protein yesQ [Bacillus subtilis]


1286
YesR protein [Bacillus subtilis]
UniRef100_O31521

Bacillus subtilis

YesR


1287
YesS protein [Bacillus subtilis]
UniRef100_O31522

Bacillus subtilis

YesS


1288
YesT protein [Bacillus subtilis]
UniRef100_O31523

Bacillus subtilis

YesT


1289



YesU


1290
YesV protein [Bacillus subtilis]
UniRef100_O31525

Bacillus subtilis

YesV


1291
YesW protein [Bacillus subtilis]
UniRef100_O31526

Bacillus subtilis

YesW


1292


1293



YesT


1294
YesX protein [Bacillus subtilis]
UniRef100_O31527

Bacillus subtilis

YesX


1295
Putative ion-channel protein
UniRef100_Q8Z4X6

Salmonella typhi

YccK



[Salmonella typhi]


1296
YesY protein [Bacillus subtilis]
UniRef100_O31528

Bacillus subtilis

YesY


1297
YesZ protein [Bacillus subtilis]
UniRef100_O31529

Bacillus subtilis

YesZ


1298



YetA


1299
Lipoprotein lplA precursor [Bacillus
UniRef100_P37966

Bacillus subtilis

LplA




subtilis]



1300
LplB protein [Bacillus subtilis]
UniRef100_P39128

Bacillus subtilis

LplB


1301
LplC protein [Bacillus subtilis]
UniRef100_P39129

Bacillus subtilis

LplC


1302
YkbA protein [Bacillus subtilis]
UniRef100_O34739

Bacillus subtilis

YkbA


1303
YkcA protein [Bacillus subtilis]
UniRef100_O34689

Bacillus subtilis

YkcA


1304
Hypothetical protein [Bacillus cereus]
UniRef100_Q81CP9

Bacillus cereus



1305
Probable serine protease do-like htrA
UniRef100_O34358

Bacillus subtilis

HtrA



[Bacillus subtilis]


1306
Pyrroline-5-carboxylate reductase 3
UniRef100_Q00777

Bacillus subtilis

ProG



[Bacillus subtilis]


1307
D-aminopeptidase [Bacillus subtilis]
UniRef100_P26902

Bacillus subtilis

DppA


1308
Dipeptide transport system permease
UniRef100_P26903

Bacillus subtilis

DppB



protein dppB [Bacillus subtilis]


1309
Dipeptide transport system permease
UniRef100_P26904

Bacillus subtilis

DppC



protein dppC [Bacillus subtilis]


1310
Dipeptide transport ATP-binding protein
UniRef100_P26905

Bacillus subtilis

DppD



dppD [Bacillus subtilis]


1311
Dipeptide-binding protein dppE
UniRef100_P26906

Bacillus subtilis

DppE



precursor [Bacillus subtilis]


1312
Hypothetical protein ykfA [Bacillus
UniRef100_O34851

Bacillus subtilis

YkfA




subtilis]



1313
YkfB [Bacillus subtilis]
UniRef100_O34508

Bacillus subtilis

YkfB


1314
YkfC [Bacillus subtilis]
UniRef100_O35010

Bacillus subtilis

YkfC


1315
YkfD [Bacillus subtilis]
UniRef100_O34480

Bacillus subtilis

YkfD


1316
BH1779 protein [Bacillus halodurans]
UniRef100_Q9KBZ5

Bacillus

YkgA






halodurans



1317
Putative acyl-CoA thioester hydrolase
UniRef100_P49851

Bacillus subtilis

YkhA



ykhA [Bacillus subtilis]


1318



YkjA


1319
Pectate lyase 47 precursor [Bacillus sp.
UniRef100_Q9AJM4

Bacillus sp. TS-

Pel



TS-47]

47


1320
Transcriptional regulator, PadR family
UniRef100_Q6HED5

Bacillus thuringiensis




[Bacillus thuringiensis]


1321
Hypothetical protein OB0568
UniRef100_Q8ESQ3

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


1322
BH1312 protein [Bacillus halodurans]
UniRef100_Q9KDA2

Bacillus halodurans



1323
Hypothetical protein ykkA [Bacillus
UniRef100_P49854

Bacillus subtilis

YkkA




subtilis]



1324
Hypothetical protein ykkC [Bacillus
UniRef100_P49856

Bacillus subtilis

YkkC




subtilis]



1325


1326
YkkE [Bacillus subtilis]
UniRef100_O34990

Bacillus subtilis

YkkE


1327
Glutamate 5-kinase 1 [Bacillus subtilis]
UniRef100_P39820

Bacillus subtilis

ProB


1328
Gamma-glutamyl phosphate reductase
UniRef100_P39821

Bacillus subtilis

ProA



[Bacillus subtilis]


1329
Organic hydroperoxide resistance
UniRef100_O34762

Bacillus subtilis

YklA



protein ohrA [Bacillus subtilis]


1330
Organic hydroperoxide resistance
UniRef100_O34777

Bacillus subtilis

YkmA



transcriptional regulator [Bacillus




subtilis]



1331
Organic hydroperoxide resistance
UniRef100_P80242

Bacillus subtilis

YkzA



protein ohrB [Bacillus subtilis]


1332


1333
Guanine deaminase [Bacillus subtilis]
UniRef100_O34598

Bacillus subtilis

GuaD


1334
Phosphoglycerate mutase [Bacillus
UniRef100_Q9ALU0

Bacillus

YhfR




stearothermophilus]



stearothermophilus



1335


1336


1337
5-methyltetrahydropteroyltriglutamate--
UniRef100_P80877

Bacillus subtilis

MetE



homocysteine methyltransferase



[Bacillus subtilis]


1338
Intracellular serine protease [Bacillus
UniRef100_Q69DB4

Bacillus sp.

IspA



sp. WRD-2]

WRD-2


1339


1340
YkoK [Bacillus subtilis]
UniRef100_O34442

Bacillus subtilis

YkoK


1341


1342
Integrase [Oceanobacillus iheyensis]
UniRef100_Q8ETV2

Oceanobacillus

YdcL






iheyensis



1343



YqaB


1344
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81UE0

Bacillus anthracis



1345



YonS


1346


1347
Putative HTH-type transcriptional
UniRef100_P45902

Bacillus subtilis

YqaE



regulator yqaE [Bacillus subtilis]


1348
Transcriptional regulator
UniRef100_Q7P886

Fusobacterium nucleatum




[Fusobacterium nucleatum subsp.

subsp. vincentii ATCC 49256




vincentii ATCC 49256]



1349


1350


1351
Lin1236 protein [Listeria innocua]
UniRef100_Q92CD6

Listeria innocua



1352


1353


1354


1355



YqaJ


1356
35 protein [Bacteriophage SPP1]
UniRef100_Q38143
Bacteriophage
YqaK





SPP1


1357



YqaL


1358



YqaM


1359
Hypothetical protein yqaO [Bacillus
UniRef100_P45912

Bacillus subtilis





subtilis]



1360
Hypothetical protein yopY
UniRef100_O64108
Bacteriophage SPBc2



[Bacteriophage SPBc2]


1361


1362


1363


1364
Hypothetical protein CTC02137
UniRef100_Q892G2

Clostridium tetani




[Clostridium tetani]


1365
Hypothetical protein MW1918
UniRef100_Q8NVN5

Staphylococcus

YqaN



[Staphylococcus aureus]


aureus



1366


1367
Single-strand binding protein 2 [Listeria
UniRef100_Q8Y4X1

Listeria monocytogenes





monocytogenes]



1368


1369


1370
Hypothetical protein yqaQ [Bacillus
UniRef100_P45948

Bacillus subtilis

YqaQ




subtilis]



1371


1372
Hypothetical protein yqaS [Bacillus
UniRef100_P45915

Bacillus subtilis

YqaS




subtilis]



1373
Hypothetical protein yqaT [Bacillus
UniRef100_P45916

Bacillus subtilis

YqaT




subtilis]



1374
Hypothetical phage associated protein
UniRef100_Q8K610

Streptococcus pyogenes




SpyM3_1326 [Streptococcus pyogenes]


1375
Minor head structural component GP7
UniRef100_Q38442
Bacteriophage SPP1



[Bacteriophage SPP1]


1376


1377


1378


1379
Hypothetical protein CTC01553
UniRef100_Q894J0

Clostridium tetani




[Clostridium tetani]


1380
Major capsid protein [Bacteriophage
UniRef100_Q9T1B7
Bacteriophage A118



A118]


1381
ORF28 [Bacteriophage phi-105]
UniRef100_Q9ZXF5
Bacteriophage phi-105


1382


1383
15 protein [Bacteriophage SPP1]
UniRef100_Q38584
Bacteriophage SPP1


1384
Complete nucleotide sequence
UniRef100_O48446
Bacteriophage SPP1



[Bacteriophage SPP1]


1385


1386
Complete nucleotide sequence
UniRef100_O48448
Bacteriophage SPP1



[Bacteriophage SPP1]


1387
Complete nucleotide sequence
UniRef100_O48449
Bacteriophage SPP1



[Bacteriophage SPP1]


1388


1389
Complete nucleotide sequence
UniRef100_O48453
Bacteriophage SPP1



[Bacteriophage SPP1]


1390


1391
Complete nucleotide sequence
UniRef100_O48455
Bacteriophage
XkdO



[Bacteriophage SPP1]

SPP1


1392
Complete nucleotide sequence
UniRef100_O48459
Bacteriophage SPP1



[Bacteriophage SPP1]


1393
Complete nucleotide sequence
UniRef100_O48463
Bacteriophage SPP1



[Bacteriophage SPP1]


1394


1395


1396
LycA [Clostridium botulinum]
UniRef100_Q6R100

Clostridium botulinum



1397
Hypothetical protein yrkC [Bacillus
UniRef100_P54430

Bacillus subtilis

YrkC




subtilis]



1398



YhjR


1399
YdfS protein [Bacillus subtilis]
UniRef100_P96697

Bacillus subtilis

YdfS


1400
HTH-type transcriptional regulator tnrA
UniRef100_Q45666

Bacillus subtilis

TnrA



[Bacillus subtilis]


1401
Hypothetical protein ykzB [Bacillus
UniRef100_O34923

Bacillus subtilis





subtilis]



1402


1403
YkoM [Bacillus subtilis]
UniRef100_O34949

Bacillus subtilis

YkoM


1404
YkoU protein [Bacillus subtilis]
UniRef100_O34398

Bacillus subtilis

YkoU


1405
YkoV protein [Bacillus subtilis]
UniRef100_O34859

Bacillus subtilis

YkoV


1406
Signaling protein ykoW [Bacillus
UniRef100_O34311

Bacillus subtilis

YkoW




subtilis]



1407
YkoX protein [Bacillus subtilis]
UniRef100_O34908

Bacillus subtilis

YkoX


1408
YkoY protein [Bacillus subtilis]
UniRef100_O34997

Bacillus subtilis

YkoY


1409
RNA polymerase sigma factor [Bacillus
UniRef100_O31654

Bacillus subtilis

SigI




subtilis]



1410



YkrI


1411
Small, acid-soluble spore protein C3
UniRef100_P10572

Bacillus megaterium




[Bacillus megaterium]


1412
YkrK protein [Bacillus subtilis]
UniRef100_O31656

Bacillus subtilis

YkrK


1413
Probable protease htpX homolog
UniRef100_O31657

Bacillus subtilis

YkrL



[Bacillus subtilis]


1414
YkrM protein [Bacillus subtilis]
UniRef100_O31658

Bacillus subtilis

YkrM


1415
Penicillin-binding protein 3 [Bacillus
UniRef100_P42971

Bacillus subtilis

PbpC




subtilis]



1416
Hypothetical protein [Bacillus cereus]
UniRef100_Q73BI4

Bacillus cereus



1417


1418
YkrP protein [Bacillus subtilis]
UniRef100_O31660

Bacillus subtilis

YkrP


1419
Two-component sensor histidine kinase
UniRef100_O31661

Bacillus subtilis

KinE



[Bacillus subtilis]


1420
Methylated-DNA--protein-cysteine
UniRef100_P11742

Bacillus subtilis

Ogt



methyltransferase [Bacillus subtilis]


1421


1422
Methylthioribose-1-phosphate
UniRef100_O31662

Bacillus subtilis

YkrS



isomerase [Bacillus subtilis]


1423
Methylthioribose kinase [Bacillus
UniRef100_O31663

Bacillus subtilis

YkrT




subtilis]



1424
YkrU protein [Bacillus subtilis]
UniRef100_O31664

Bacillus subtilis

YkrU


1425
Transaminase mtnE [Bacillus subtilis]
UniRef100_O31665

Bacillus subtilis

YkrV


1426
2,3-diketo-5-methylthiopentyl-1-
UniRef100_O31666

Bacillus subtilis

YkrW



phosphate enolase [Bacillus subtilis]


1427
Methylthioribulose-1-phosphate
UniRef100_O31668

Bacillus subtilis

YkrY



dehydratase [Bacillus subtilis]


1428
1,2-dihydroxy-3-keto-5-
UniRef100_O31669

Bacillus subtilis

YkrZ



methylthiopentene dioxygenase



[Bacillus subtilis]


1429
Metallothiol transferase fosB
UniRef100_Q8CXK5

Oceanobacillus

YndN



[Oceanobacillus iheyensis]


iheyensis



1430
YkvA protein [Bacillus subtilis]
UniRef100_O31670

Bacillus subtilis



1431
Stage 0 sporulation regulatory protein
UniRef100_P05043

Bacillus subtilis




[Bacillus subtilis]


1432
Two-component sensor histidine kinase
UniRef100_O31671

Bacillus subtilis

KinD



[Bacillus subtilis]


1433



YkvE


1434
Chemotaxis motB protein [Bacillus
UniRef100_P28612

Bacillus subtilis

MotB




subtilis]



1435
Chemotaxis motA protein [Bacillus
UniRef100_P28611

Bacillus subtilis

MotA




subtilis]



1436
ATP-dependent Clp protease-like
UniRef100_O31673

Bacillus subtilis

ClpE



[Bacillus subtilis]


1437
YkvI protein [Bacillus subtilis]
UniRef100_O31674

Bacillus subtilis

YkvI


1438
YkvJ protein [Bacillus subtilis]
UniRef100_O31675

Bacillus subtilis

YkvJ


1439
YkvK protein [Bacillus subtilis]
UniRef100_O31676

Bacillus subtilis

YkvK


1440
YkvL protein [Bacillus subtilis]
UniRef100_O31677

Bacillus subtilis

YkvL


1441
YkvM protein [Bacillus subtilis]
UniRef100_O31678

Bacillus subtilis

YkvM


1442
DNA integration/recombination protein
UniRef100_Q894H7

Clostridium tetani

CodV



[Clostridium tetani]


1443
Integrase/recombinase [Bacillus cereus
UniRef100_Q633V7

Bacillus cereus

RipX



ZK]

ZK


1444


1445


1446


1447
YoqV protein [Bacteriophage SPBc2]
UniRef100_O64130
Bacteriophage
LigB





SPBc2


1448


1449


1450
UPI00003CC586 UniRef100 entry
UniRef100_UPI00003CC586


1451


1452


1453


1454


1455
Prophage LambdaBa02, HNH
UniRef100_Q81W86

Bacillus anthracis




endonuclease family protein [Bacillus




anthracis]



1456
Terminase small subunit
UniRef100_Q6GAL5

Staphylococcus aureus




[Staphylococcus aureus]


1457
Prophage LambdaBa02, terminase,
UniRef100_Q6HUD2

Bacillus anthracis




large subunit, putative [Bacillus




anthracis]



1458
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81W89

Bacillus anthracis



1459
ClpP family serine protease, possible
UniRef100_Q97HW4

Clostridium

ClpP



phage related [Clostridium


acetobutylicum





acetobutylicum]



1460
Prophage LambdaBa02, major capsid
UniRef100_Q81W91

Bacillus anthracis




protein, putative [Bacillus anthracis]


1461
Precursor polypeptide (AA −37 to 1647)
UniRef100_Q03658
unidentified bacterium



precursor [unidentified bacterium]


1462
Gp7 protein [Bacteriophage phi3626]
UniRef100_Q8SBP7
Bacteriophage phi3626


1463
Uncharacterized phage related protein
UniRef100_Q97HW7

Clostridium acetobutylicum




[Clostridium acetobutylicum]


1464
Hypothetical protein CAC1887
UniRef100_Q97HW9

Clostridium acetobutylicum




[Clostridium acetobutylicum]


1465
Prophage LambdaBa02, major tail
UniRef100_Q81W97

Bacillus anthracis




protein, putative [Bacillus anthracis]


1466


1467


1468
Prophage LambdaBa02, tape measure
UniRef100_Q81WA0

Bacillus

YqbO



protein, putative [Bacillus anthracis]


anthracis



1469
Lin2382 protein [Listeria innocua]
UniRef100_Q928Z8

Listeria innocua



1470
Protein gp18 [Listeria monocytogenes]
UniRef100_Q8Y4Z4

Listeria monocytogenes



1471



YclG


1472



XkdV


1473



XkdW


1474
YomP protein [Bacteriophage SPBc2]
UniRef100_O64052
Bacteriophage SPBc2


1475
Glycerophosphoryl diester
UniRef100_Q737E6

Bacillus cereus

GlpQ



phosphodiesterase, putative [Bacillus




cereus]



1476
Protein bhlA [Bacteriophage SPBc2]
UniRef100_O64039
Bacteriophage SPBc2


1477
ORF46 [Bacteriophage phi-105]
UniRef100_Q9ZXD7
Bacteriophage
XlyB





phi-105


1478


1479


1480


1481
Transcriptional regulator, DeoR family
UniRef100_Q816D5

Bacillus cereus




[Bacillus cereus]


1482
Hypothetical protein yolD
UniRef100_O64030
Bacteriophage SPBc2



[Bacteriophage SPBc2]


1483
DNA integration/recombination/invertion
UniRef100_Q81GD4

Bacillus cereus

YdcL



protein [Bacillus cereus]


1484
YkvM protein [Bacillus subtilis]
UniRef100_O31678

Bacillus subtilis



1485
Response regulator aspartate
UniRef100_P40771

Bacillus subtilis

RapH



phosphatase H [Bacillus subtilis]


1486
YoaT [Bacillus subtilis]
UniRef100_O34535

Bacillus subtilis

YoaT


1487
YozG protein [Bacillus subtilis]
UniRef100_O31834

Bacillus subtilis



1488
YoaS protein [Bacillus subtilis]
UniRef100_O31833

Bacillus subtilis

YoaS


1489


1490


1491


1492


1493


1494
YkvS protein [Bacillus subtilis]
UniRef100_O31684

Bacillus subtilis



1495
BH2327 protein [Bacillus halodurans]
UniRef100_Q9KAG0

Bacillus halodurans



1496
YkvT protein [Bacillus subtilis]
UniRef100_O31685

Bacillus subtilis

YkvT


1497
YkvU protein [Bacillus subtilis]
UniRef100_O31686

Bacillus subtilis

YkvU


1498
YkvV protein [Bacillus subtilis]
UniRef100_O31687

Bacillus subtilis

YkvV


1499



YkvW


1500
YkvY protein [Bacillus subtilis]
UniRef100_O31689

Bacillus subtilis

YkvY


1501
Necrosis and ethylene inducing protein
UniRef100_Q9KFT2

Bacillus halodurans




[Bacillus halodurans]


1502
Putative HTH-type transcriptional
UniRef100_O31690

Bacillus subtilis

YkvZ



regulator ykvZ [Bacillus subtilis]


1503
Transcription antiterminator [Bacillus
UniRef100_O06710

Bacillus subtilis

GlcT




subtilis]



1504



PtsG


1505
Phosphocarrier protein HPr [Bacillus
UniRef100_P08877

Bacillus subtilis





subtilis]



1506
Phosphoenolpyruvate-protein
UniRef100_P08838

Bacillus subtilis

PtsI



phosphotransferase [Bacillus subtilis]


1507
SplA [Bacillus amyloliquefaciens]
UniRef100_O54358

Bacillus amyloliquefaciens



1508
Spore photoproduct lyase [Bacillus
UniRef100_O54359

Bacillus

SplB




amyloliquefaciens]



amyloliquefaciens



1509
Hypothetical protein orf1 [Bacillus
UniRef100_O05187

Bacillus subtilis

YkwB




subtilis]



1510
Methyl-accepting chemotaxis protein
UniRef100_P54576

Bacillus subtilis

McpC



mcpC [Bacillus subtilis]


1511
3-oxoacyl-(Acyl carrier protein)
UniRef100_Q8EMP9

Oceanobacillus

FabG



reductase [Oceanobacillus iheyensis]


iheyensis



1512
Hypothetical oxidoreductase ykwC
UniRef100_O34948

Bacillus subtilis

YkwC



[Bacillus subtilis]


1513
YkwD protein [Bacillus subtilis]
UniRef100_O31694

Bacillus subtilis

YkwD


1514
YkuA protein [Bacillus subtilis]
UniRef100_O31399

Bacillus subtilis

YkuA


1515
Sporulation kinase A [Bacillus subtilis]
UniRef100_P16497

Bacillus subtilis

KinA


1516
Putative aminotransferase A [Bacillus
UniRef100_P16524

Bacillus subtilis

PatA




subtilis]



1517


1518
Hypothetical protein yxaI [Bacillus
UniRef100_P42108

Bacillus subtilis

YxaI




subtilis]



1519



YxiO


1520
Chemotaxis protein cheV [Bacillus
UniRef100_P37599

Bacillus subtilis

CheV




subtilis]



1521
Hypothetical protein ykyB [Bacillus
UniRef100_P42430

Bacillus subtilis

YkyB




subtilis]



1522



YkuC


1523
YkuD protein [Bacillus subtilis]
UniRef100_O34816

Bacillus subtilis

YkuD


1524



YkuE


1525
Hypothetical oxidoreductase ykuF
UniRef100_O34717

Bacillus subtilis

YkuF



[Bacillus subtilis]


1526
YkuI protein [Bacillus subtilis]
UniRef100_O35014

Bacillus subtilis

YkuI


1527


1528
YkuJ protein [Bacillus subtilis]
UniRef100_O34588

Bacillus subtilis



1529
YkuK protein [Bacillus subtilis]
UniRef100_O34776

Bacillus subtilis

YkuK


1530
Hypothetical protein ykzF [Bacillus
UniRef100_O31697

Bacillus subtilis





subtilis]



1531
YkuL protein [Bacillus subtilis]
UniRef100_O31698

Bacillus subtilis

YkuL


1532
Putative HTH-type transcriptional
UniRef100_O34827

Bacillus subtilis

CcpC



regulator ykuM [Bacillus subtilis]


1533
Probable flavodoxin 1 [Bacillus subtilis]
UniRef100_O34737

Bacillus subtilis

YkuN


1534
YkuO protein [Bacillus subtilis]
UniRef100_O34879

Bacillus subtilis

YkuO


1535
Probable flavodoxin 2 [Bacillus subtilis]
UniRef100_O34589

Bacillus subtilis

YkuP


1536
YkuQ protein [Bacillus subtilis]
UniRef100_O34981

Bacillus subtilis

YkuQ


1537
YkuR protein [Bacillus subtilis]
UniRef100_O34916

Bacillus subtilis

YkuR


1538
Hypothetical UPF0180 protein ykuS
UniRef100_O34783

Bacillus subtilis




[Bacillus subtilis]


1539
YkuU protein [Bacillus subtilis]
UniRef100_O34564

Bacillus subtilis

YkuU


1540
YkuV protein [Bacillus subtilis]
UniRef100_O31403

Bacillus subtilis

YkuV


1541
Repressor rok [Bacillus subtilis]
UniRef100_O34857

Bacillus subtilis

Rok


1542
YknT protein [Bacillus subtilis]
UniRef100_O31700

Bacillus subtilis

YknT


1543



MobA


1544
Molybdopterin biosynthesis protein
UniRef100_O31702

Bacillus subtilis

MoeB



MoeB [Bacillus subtilis]


1545
Molybdopterin biosynthesis protein
UniRef100_O31703

Bacillus subtilis

MoeA



MoeA [Bacillus subtilis]


1546
Molybdopterin-guanine dinucleotide
UniRef100_O31704

Bacillus subtilis

MobB



biosynthesis protein B [Bacillus subtilis]


1547
Molybdopterin converting factor subunit
UniRef100_O31705

Bacillus subtilis

MoaE



2 [Bacillus subtilis]


1548
Molybdopterin converting factor,
UniRef100_O31706

Bacillus subtilis




subunit 1 [Bacillus subtilis]


1549


1550
YknU protein [Bacillus subtilis]
UniRef100_O31707

Bacillus subtilis

YknU


1551
YknV protein [Bacillus subtilis]
UniRef100_O31708

Bacillus subtilis

YknV


1552
Hypothetical protein yknW [Bacillus
UniRef100_O31709

Bacillus subtilis

YknW




subtilis]



1553
YknX protein [Bacillus subtilis]
UniRef100_O31710

Bacillus subtilis

YknX


1554
YknY protein [Bacillus subtilis]
UniRef100_O31711

Bacillus subtilis

YknY


1555
Hypothetical protein yknZ [Bacillus
UniRef100_O31712

Bacillus subtilis

YknZ




subtilis]



1556



FruR


1557
1-phosphofructokinase [Bacillus
UniRef100_O31714

Bacillus subtilis

FruK




subtilis]



1558
Phosphotransferase system (PTS)
UniRef100_P71012

Bacillus subtilis

FruA



fructose-specific enzyme IIABC



component [Bacillus subtilis]


1559
Signal peptidase I T [Bacillus subtilis]
UniRef100_P71013

Bacillus subtilis

SipT


1560
Hypothetical protein ykoA [Bacillus
UniRef100_O31715

Bacillus subtilis





subtilis]



1561


1562
YkpA protein [Bacillus subtilis]
UniRef100_O31716

Bacillus subtilis

YkpA


1563
BH1921 protein [Bacillus halodurans]
UniRef100_Q9KBK5

Bacillus halodurans



1564
Aminopeptidase ampS [Bacillus subtilis]
UniRef100_P39762

Bacillus subtilis

AmpS


1565


1566
MreBH protein [Bacillus subtilis]
UniRef100_P39763

Bacillus subtilis

MreBH


1567


1568
Sporulation kinase C [Bacillus subtilis]
UniRef100_P39764

Bacillus subtilis

KinC


1569
Hypothetical protein ykqB [Bacillus
UniRef100_P39760

Bacillus subtilis

YkqB




subtilis]



1570
Adenine deaminase [Bacillus subtilis]
UniRef100_P39761

Bacillus subtilis

AdeC


1571



YkqC


1572
YkzG protein [Bacillus subtilis]
UniRef100_O31718

Bacillus subtilis



1573
Hypothetical protein ykrA [Bacillus
UniRef100_Q45494

Bacillus subtilis

YkrA




subtilis]



1574



YkrB


1575


1576
Hypothetical protein ykyA [Bacillus
UniRef100_P21884

Bacillus subtilis

YkyA




subtilis]



1577
Pyruvate dehydrogenase E1
UniRef100_P21881

Bacillus subtilis

PdhA



component, alpha subunit [Bacillus




subtilis]



1578
Pyruvate dehydrogenase E1
UniRef100_P21882

Bacillus subtilis

PdhB



component, beta subunit [Bacillus




subtilis]



1579
Dihydrolipoyllysine-residue
UniRef100_P21883

Bacillus subtilis

PdhC



acetyltransferase component of



pyruvate dehydrogenase complex



[Bacillus subtilis]


1580
Dihydrolipoyl dehydrogenase [Bacillus
UniRef100_P21880

Bacillus subtilis

PdhD




subtilis]



1581
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069


1582
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012





anthracis str. A2012]



1583


1584


1585
Arginine decarboxylase [Bacillus
UniRef100_P21885

Bacillus subtilis

SpeA




subtilis]



1586
Hypothetical UPF0223 protein yktA
UniRef100_Q45497

Bacillus subtilis




[Bacillus subtilis]


1587
Hypothetical protein yktB [Bacillus
UniRef100_Q45498

Bacillus subtilis

YktB




subtilis]



1588
YkzI protein [Bacillus subtilis]
UniRef100_O31719

Bacillus subtilis



1589
Inositol-1-monophosphatase [Bacillus
UniRef100_Q45499

Bacillus subtilis

YktC




subtilis]



1590
Hypothetical protein ykzC [Bacillus
UniRef100_O31720

Bacillus subtilis

YkzC




subtilis]



1591
Hypothetical protein ylaA [Bacillus
UniRef100_O07625

Bacillus subtilis

YlaA




subtilis]



1592
Hypothetical protein ylaB [Bacillus
UniRef100_O07626

Bacillus subtilis





subtilis]



1593
YlaC protein [Bacillus subtilis]
UniRef100_O07627

Bacillus subtilis

YlaC


1594
Hypothetical protein ylaD [Bacillus
UniRef100_O07628

Bacillus subtilis





subtilis]



1595
Hypothetical protein ylaF [Bacillus
UniRef100_O07630

Bacillus subtilis





subtilis]



1596
GTP-binding protein typA/bipA homolog
UniRef100_O07631

Bacillus subtilis

YlaG



[Bacillus subtilis]


1597
YlaH protein [Bacillus subtilis]
UniRef100_O07632

Bacillus subtilis

YlaH


1598
YhzA homolog [Bacillus subtilis]
UniRef100_O07562

Bacillus subtilis

YhjH


1599
Hypothetical protein yhjG [Bacillus
UniRef100_O07561

Bacillus subtilis

YhjG




subtilis]



1600


1601
Hypothetical lipoprotein ylaJ precursor
UniRef100_O07634

Bacillus subtilis

YlaJ



[Bacillus subtilis]


1602
YlaK protein [Bacillus subtilis]
UniRef100_O07635

Bacillus subtilis

YlaK


1603
UPI00003CB7B1 UniRef100 entry
UniRef100_UPI00003CB7B1

YlaL


1604
Probable glutaminase ylaM [Bacillus
UniRef100_O07637

Bacillus subtilis

YlaM




subtilis]



1605
YlaN protein [Bacillus subtilis]
UniRef100_O07638

Bacillus subtilis



1606
Hypothetical protein ylaO [Bacillus
UniRef100_O07639

Bacillus subtilis

FtsW




subtilis]



1607



PycA


1608
Cytochrome AA3 controlling protein
UniRef100_P12946

Bacillus subtilis

CtaA



[Bacillus subtilis]


1609
Protoheme IX farnesyltransferase
UniRef100_P24009

Bacillus subtilis

CtaB



[Bacillus subtilis]


1610
Cytochrome c oxidase polypeptide II
UniRef100_P24011

Bacillus subtilis

CtaC



precursor (EC 1.9.3.1) (Cytochrome



aa3 subunit 2) (Caa-3605 subunit 2)



(Oxidase aa(3) subunit 2) [Bacillus




subtilis]



1611
Cytochrome c oxidase polypeptide I
UniRef100_P24010

Bacillus subtilis

CtaD



(EC 1.9.3.1) (Cytochrome aa3 subunit



1) (Caa-3605 subunit 1) (Oxidase aa(3)



subunit 1) [Bacillus subtilis]


1612
Cytochrome c oxidase polypeptide III
UniRef100_P24012

Bacillus subtilis

CtaE



(EC 1.9.3.1) (Cytochrome aa3 subunit



3) (Caa-3605 subunit 3) (Oxidase aa(3)



subunit 3) [Bacillus subtilis]


1613
Cytochrome c oxidase polypeptide IVB
UniRef100_P24013

Bacillus subtilis

CtaF



[Bacillus subtilis]


1614
CtaG protein [Bacillus subtilis]
UniRef100_O34329

Bacillus subtilis

CtaG


1615
YlbA protein [Bacillus subtilis]
UniRef100_O34743

Bacillus subtilis

YlbA


1616
YlbB protein [Bacillus subtilis]
UniRef100_O34682

Bacillus subtilis

YlbB


1617
YlbC protein [Bacillus subtilis]
UniRef100_O34586

Bacillus subtilis

YlbC


1618
YlbD protein [Bacillus subtilis]
UniRef100_O34880

Bacillus subtilis

YlbD


1619
YlbE protein [Bacillus subtilis]
UniRef100_O34958

Bacillus subtilis



1620
Regulatory protein ylbF [Bacillus
UniRef100_O34412

Bacillus subtilis

YlbF




subtilis]



1621
Hypothetical UPF0298 protein ylbG
UniRef100_O34658

Bacillus subtilis




[Bacillus subtilis]


1622
YlbH protein [Bacillus subtilis]
UniRef100_O34331

Bacillus subtilis

YlbH


1623
Phosphopantetheine
UniRef100_O34797

Bacillus subtilis

YlbI



adenylyltransferase [Bacillus subtilis]


1624



YlbJ


1625
YlbL protein [Bacillus subtilis]
UniRef100_O34470

Bacillus subtilis

YlbL


1626
YlbM protein [Bacillus subtilis]
UniRef100_O34513

Bacillus subtilis

YlbM


1627
YlbN protein [Bacillus subtilis]
UniRef100_O34445

Bacillus subtilis

YlbN


1628
50S ribosomal protein L32 [Bacillus
UniRef100_O34687

Bacillus subtilis





subtilis]



1629
Hypothetical protein ylbO [Bacillus
UniRef100_O34549

Bacillus subtilis

YlbO




subtilis]



1630
YlbP protein [Bacillus subtilis]
UniRef100_O34468

Bacillus subtilis

YlbP


1631
Probable 2-dehydropantoate 2-
UniRef100_O34661

Bacillus subtilis

YlbQ



reductase [Bacillus subtilis]


1632



YllA


1633
Protein mraZ [Bacillus subtilis]
UniRef100_P55343

Bacillus subtilis

YllB


1634
S-adenosyl-methyltransferase mraW
UniRef100_Q07876

Bacillus subtilis

YlxA



[Bacillus subtilis]


1635



FtsL


1636
Penicillin-binding protein 2B [Bacillus
UniRef100_Q07868

Bacillus subtilis

PbpB




subtilis]



1637
Stage V sporulation protein D [Bacillus
UniRef100_Q03524

Bacillus subtilis

SpoVD




subtilis]



1638
UDP-N-acetylmuramoylalanyl-D-
UniRef100_Q03523

Bacillus subtilis

MurE



glutamate--2,6-diaminopimelate ligase



[Bacillus subtilis]


1639
Phospho-N-acetylmuramoyl-
UniRef100_Q03521

Bacillus subtilis

MraY



pentapeptide-transferase [Bacillus




subtilis]



1640
UDP-N-acetylmuramoylalanine--D-
UniRef100_Q03522

Bacillus subtilis

MurD



glutamate ligase [Bacillus subtilis]


1641
Stage V sporulation protein E [Bacillus
UniRef100_P07373

Bacillus subtilis

SpoVE




subtilis]



1642
UDP-N-acetylglucosamine--N-
UniRef100_P37585

Bacillus subtilis

MurG



acetylmuramyl-(pentapeptide)pyrophosphoryl-



undecaprenol N-



acetylglucosamine transferase [Bacillus




subtilis]



1643
UDP-N-acetylenolpyruvoylglucosamine
UniRef100_P18579

Bacillus subtilis

MurB



reductase [Bacillus subtilis]


1644



DivIB


1645



YlxW


1646



YlxX


1647



Sbp


1648



FtsA


1649
Cell division protein ftsZ [Bacillus
UniRef100_P17865

Bacillus subtilis

FtsZ




subtilis]



1650



Bpr


1651
Bacillopeptidase F precursor [Bacillus
UniRef100_P16397

Bacillus subtilis

Bpr




subtilis]



1652
Sporulation sigma-E factor processing
UniRef100_P13801

Bacillus subtilis

SpoIIGA



peptidase [Bacillus subtilis]


1653
RNA polymerase sigma-E factor
UniRef100_P06222

Bacillus subtilis

SigE



precursor [Bacillus subtilis]


1654
RNA polymerase sigma-G factor
UniRef100_P19940

Bacillus subtilis

SigG



[Bacillus subtilis]


1655
YlmA protein [Bacillus subtilis]
UniRef100_O31723

Bacillus subtilis

YlmA


1656


1657
YlmC protein [Bacillus subtilis]
UniRef100_O31725

Bacillus subtilis



1658
Hypothetical UPF0124 protein ylmD
UniRef100_O31726

Bacillus subtilis

YlmD



[Bacillus subtilis]


1659



YlmE


1660
YlmF protein [Bacillus subtilis]
UniRef100_O31728

Bacillus subtilis

YlmF


1661
YlmG protein [Bacillus subtilis]
UniRef100_O31729

Bacillus subtilis



1662
Minicell-associated protein [Bacillus
UniRef100_P71020

Bacillus subtilis

YlmH




subtilis]



1663
Minicell-associated protein DivIVA
UniRef100_P71021

Bacillus subtilis

DivIVA



[Bacillus subtilis]


1664
Isoleucyl-tRNA synthetase [Bacillus
UniRef100_Q45477

Bacillus subtilis

IleS




subtilis]



1665



YlyA


1666



LspA


1667
Hypothetical pseudouridine synthase
UniRef100_Q45480

Bacillus subtilis

YlyB



ylyB [Bacillus subtilis]


1668
PyrR bifunctional protein [Includes:
UniRef100_P39765
Includes:
PyrR



Pyrimidine operon regulatory protein;

Pyrimidine



Uracil phosphoribosyltransferase (EC

operon



2.4.2.9) (UPRTase)] [Bacillus subtilis]

regulatory





protein; Uracil





phosphoribosyltransferase





(EC





2.4.2.9)





(UPRTase)


1669
Uracil permease [Bacillus subtilis]
UniRef100_P39766

Bacillus subtilis

PyrP


1670
Aspartate carbamoyltransferase
UniRef100_P05654

Bacillus subtilis

PyrB



[Bacillus subtilis]


1671
Dihydroorotase [Bacillus subtilis]
UniRef100_P25995

Bacillus subtilis

PyrC


1672
Carbamoyl-phosphate synthase,
UniRef100_P25993

Bacillus subtilis

PyrAA



pyrimidine-specific, small chain



[Bacillus subtilis]


1673
Carbamoyl-phosphate synthase,
UniRef100_P25994

Bacillus subtilis

PyrAB



pyrimidine-specific, large chain [Bacillus




subtilis]



1674
Dihydroorotate dehydrogenase electron
UniRef100_P25983

Bacillus subtilis

PyrK



transfer subunit [Bacillus subtilis]


1675
Dihydroorotate dehydrogenase,
UniRef100_P25996

Bacillus subtilis

PyrD



catalytic subunit [Bacillus subtilis]


1676
Orotidine 5′-phosphate decarboxylase
UniRef100_P25971

Bacillus subtilis

PyrF



[Bacillus subtilis]


1677
Orotate phosphoribosyltransferase
UniRef100_P25972

Bacillus subtilis

PyrE



[Bacillus subtilis]


1678


1679



CysH


1680
YlnA protein [Bacillus subtilis]
UniRef100_O34734

Bacillus subtilis

CysP


1681
Sulfate adenylyltransferase [Bacillus
UniRef100_O34764

Bacillus subtilis

Sat




subtilis]



1682
Probable adenylyl-sulfate kinase
UniRef100_O34577

Bacillus subtilis

CysC



[Bacillus subtilis]


1683
Putative S-adenosyl L-methionine:
UniRef100_O34744

Bacillus subtilis

YlnD



uroporphyrinogen III methyltransferase



[Bacillus subtilis]


1684
Sirohydrochlorin ferrochelatase
UniRef100_O34632

Bacillus subtilis

YlnE



[Bacillus subtilis]


1685
YlnF protein [Bacillus subtilis]
UniRef100_O34813

Bacillus subtilis

YlnF


1686
Putative fibronectin-binding protein
UniRef100_O34693

Bacillus subtilis

YloA



[Bacillus subtilis]


1687
YloB protein [Bacillus subtilis]
UniRef100_O34431

Bacillus subtilis

YloB


1688
YloC protein [Bacillus subtilis]
UniRef100_O34441

Bacillus subtilis

YloC


1689
Hypothetical UPF0296 protein ylzA
UniRef100_Q7WY72

Bacillus subtilis




[Bacillus subtilis]


1690
Guanylate kinase [Bacillus subtilis]
UniRef100_O34328

Bacillus subtilis

Gmk


1691
DNA-directed RNA polymerase omega
UniRef100_O35011

Bacillus subtilis




chain [Bacillus subtilis]


1692
YloI protein [Bacillus subtilis]
UniRef100_O35033

Bacillus subtilis

YloI


1693
Primosomal protein N′ [Bacillus subtilis]
UniRef100_P94461

Bacillus subtilis

PriA


1694
Peptide deformylase 1 [Bacillus subtilis]
UniRef100_P94462

Bacillus subtilis

Def


1695
Methionyl-tRNA formyltransferase
UniRef100_P94463

Bacillus subtilis

Fmt



[Bacillus subtilis]


1696
Ribosomal RNA small subunit
UniRef100_P94464

Bacillus subtilis

YloM



methyltransferase B (EC 2.1.1.—) (rRNA



(cytosine-C(5)-)-methyltransferase)



[Bacillus subtilis]


1697
Hypothetical UPF0063 protein yloN
UniRef100_O34617

Bacillus subtilis

YloN



[Bacillus subtilis]


1698
Protein phosphatase [Bacillus subtilis]
UniRef100_O34779

Bacillus subtilis

PrpC


1699
Probable serine/threonine-protein
UniRef100_O34507

Bacillus subtilis

PrkC



kinase yloP [Bacillus subtilis]


1700
Probable GTPase engC [Bacillus
UniRef100_O34530

Bacillus subtilis

YloQ




subtilis]



1701
Ribulose-phosphate 3-epimerase
UniRef100_O34557

Bacillus subtilis

Rpe



[Bacillus subtilis]


1702
YloS protein [Bacillus subtilis]
UniRef100_O34664

Bacillus subtilis

YloS


1703


1704
50S ribosomal protein L28 [Bacillus
UniRef100_P37807

Bacillus subtilis





subtilis]



1705
Hypothetical protein yloU [Bacillus
UniRef100_O34318

Bacillus subtilis

YloU




subtilis]



1706



YloV


1707
Probable L-serine dehydratase, beta
UniRef100_O34635

Bacillus subtilis

SdaAB



chain [Bacillus subtilis]


1708
Probable L-serine dehydratase, alpha
UniRef100_O34607

Bacillus subtilis

SdaAA



chain [Bacillus subtilis]


1709
ATP-dependent DNA helicase recG
UniRef100_O34942

Bacillus subtilis

RecG



[Bacillus subtilis]


1710
Transcription factor fapR [Bacillus
UniRef100_O34835

Bacillus subtilis

YlpC




subtilis]



1711
Fatty acid/phospholipid synthesis
UniRef100_P71018

Bacillus subtilis

PlsX



protein plsX [Bacillus subtilis]


1712
Malonyl CoA-acyl carrier protein
UniRef100_P71019

Bacillus subtilis

FabD



transacylase [Bacillus subtilis]


1713
3-oxoacyl-[acyl-carrier-protein]
UniRef100_P51831
acyl-carrier-
FabG



reductase [Bacillus subtilis]

protein


1714
Acyl carrier protein [Bacillus subtilis]
UniRef100_P80643

Bacillus subtilis



1715



Rnc


1716
Chromosome partition protein smc
UniRef100_P51834

Bacillus subtilis

Smc



[Bacillus subtilis]


1717



FtsY


1718


1719
Signal recognition particle protein
UniRef100_P37105

Bacillus subtilis

Ffh



[Bacillus subtilis]


1720
30S ribosomal protein S16 [Bacillus
UniRef100_P21474

Bacillus subtilis





subtilis]



1721


1722
YlqD protein [Bacillus subtilis]
UniRef100_O31739

Bacillus subtilis

YlqD


1723



RimM


1724



TrmD


1725
50S ribosomal protein L19 [Bacillus
UniRef100_O31742

Bacillus subtilis

RplS




subtilis]



1726
YlqF protein [Bacillus subtilis]
UniRef100_O31743

Bacillus subtilis

YlqF


1727
Ribonuclease HII [Bacillus subtilis]
UniRef100_O31744

Bacillus subtilis

RnhB


1728
YlqG protein [Bacillus subtilis]
UniRef100_O31745

Bacillus subtilis

YlqG


1729
YlqH protein [Bacillus subtilis]
UniRef100_O34867

Bacillus subtilis



1730
Succinyl-CoA synthetase beta chain
UniRef100_P80886

Bacillus subtilis

SucC



[Bacillus subtilis]


1731
Succinyl-CoA synthetase alpha chain
UniRef100_P80865

Bacillus subtilis

SucD



[Bacillus subtilis]


1732



Smf


1733



TopA


1734
Protein gid [Bacillus subtilis]
UniRef100_P39815

Bacillus subtilis

Gid


1735
Tyrosine recombinase xerC [Bacillus
UniRef100_P39776

Bacillus subtilis

CodV




subtilis]



1736
ATP-dependent protease hslV
UniRef100_P39070

Bacillus subtilis

ClpQ



precursor [Bacillus subtilis]


1737
ATP-dependent hsl protease ATP-
UniRef100_P39778

Bacillus subtilis

ClpY



binding subunit hslU [Bacillus subtilis]


1738
GTP-sensing transcriptional pleiotropic
UniRef100_P39779

Bacillus subtilis

CodY



repressor codY [Bacillus subtilis]


1739
Flagellar basal-body rod protein flgB
UniRef100_P24500

Bacillus subtilis

FlgB



[Bacillus subtilis]


1740
Flagellar basal-body rod protein flgC
UniRef100_P24501

Bacillus subtilis

FlgC



[Bacillus subtilis]


1741
Flagellar hook-basal body complex
UniRef100_P24502

Bacillus subtilis

FliE



protein fliE [Bacillus subtilis]


1742
Flagellar M-ring protein [Bacillus
UniRef100_P23447

Bacillus subtilis

FliF




subtilis]



1743
Flagellar motor switch protein fliG
UniRef100_P23448

Bacillus subtilis

FliG



[Bacillus subtilis]


1744
Probable flagellar assembly protein fliH
UniRef100_P23449

Bacillus subtilis

FliH



[Bacillus subtilis]


1745
Flagellum-specific ATP synthase
UniRef100_P23445

Bacillus subtilis

FliI



[Bacillus subtilis]


1746
Flagellar fliJ protein [Bacillus subtilis]
UniRef100_P20487

Bacillus subtilis

FliJ


1747
FlaA locus 22.9 kDa protein [Bacillus
UniRef100_P23454

Bacillus subtilis

YlxF




subtilis]



1748
Probable flagellar hook-length control
UniRef100_P23451

Bacillus subtilis

FliK



protein [Bacillus subtilis]


1749
FlaA locus hypothetical protein ylxG
UniRef100_P23455

Bacillus subtilis

YlxG



[Bacillus subtilis]


1750



FlgE


1751
BH2448 protein [Bacillus halodurans]
UniRef100_Q9KA42

Bacillus halodurans



1752

Flagellar fliL protein [Bacillus subtilis]

UniRef100_P23452

Bacillus subtilis

FliL


1753

Flagellar motor switch protein fliM

UniRef100_P23453

Bacillus subtilis

FliM



[Bacillus subtilis]


1754

Flagellar motor switch protein fliY

UniRef100_P24073

Bacillus subtilis

FliY



[Bacillus subtilis]


1755
Chemotaxis protein cheY homolog
UniRef100_P24072

Bacillus subtilis

CheY



[Bacillus subtilis]


1756

Flagellar biosynthetic protein fliZ

UniRef100_P35536

Bacillus subtilis

FliZ



precursor [Bacillus subtilis]


1757

Flagellar biosynthetic protein fliP

UniRef100_P35528

Bacillus subtilis

FliP



[Bacillus subtilis]


1758

Flagellar biosynthetic protein fliQ

UniRef100_P35535

Bacillus subtilis




[Bacillus subtilis]


1759

Flagellar biosynthetic protein fliR

UniRef100_P35537

Bacillus subtilis

FliR



[Bacillus subtilis]


1760

Flagellar biosynthetic protein flhB

UniRef100_P35538

Bacillus subtilis

FlhB



[Bacillus subtilis]


1761

Flagellar biosynthesis protein flhA

UniRef100_P35620

Bacillus subtilis

FlhA



[Bacillus subtilis]


1762

Flagellar biosynthesis protein flhF

UniRef100_Q01960

Bacillus subtilis

FlhF



[Bacillus subtilis]


1763
Hypothetical protein ylxH [Bacillus
UniRef100_P40742

Bacillus subtilis

YlxH




subtilis]



1764
Chemotaxis response regulator protein-
UniRef100_Q05522

Bacillus subtilis

CheB



glutamate methylesterase [Bacillus




subtilis]



1765
Chemotaxis protein cheA [Bacillus
UniRef100_P29072

Bacillus subtilis

CheA




subtilis]



1766
Chemotaxis protein cheW [Bacillus
UniRef100_P39802

Bacillus subtilis

CheW




subtilis]



1767
Chemotaxis protein cheC [Bacillus
UniRef100_P40403

Bacillus subtilis

CheC




subtilis]



1768
Chemotaxis protein cheD [Bacillus
UniRef100_P40404

Bacillus subtilis

CheD




subtilis]



1769
RNA polymerase sigma-D factor
UniRef100_P10726

Bacillus subtilis

SigD



[Bacillus subtilis]


1770
Swarming motility protein swrB [Bacillus
UniRef100_P40405

Bacillus subtilis

YlxL




subtilis]



1771
30S ribosomal protein S2 [Bacillus
UniRef100_P21464

Bacillus subtilis

RpsB




subtilis]



1772
Translation elongation factor Ts
UniRef100_Q65JJ8

Bacillus cereus

Tsf



[Bacillus cereus]


1773



PyrH


1774



Frr


1775
Undecaprenyl pyrophosphate
UniRef100_O31751

Bacillus subtilis

UppS



synthetase [Bacillus subtilis]


1776
Phosphatidate cytidylyltransferase
UniRef100_O31752

Bacillus subtilis

CdsA



[Bacillus subtilis]


1777
1-deoxy-D-xylulose 5-phosphate
UniRef100_O31753

Bacillus subtilis

Dxr



reductoisomerase [Bacillus subtilis]


1778
Hypothetical zinc metalloprotease yluc
UniRef100_O31754

Bacillus subtilis

YluC



[Bacillus subtilis]


1779
Prolyl-tRNA synthetase [Bacillus
UniRef100_O31755

Bacillus subtilis

ProS




subtilis]



1780
DNA polymerase III polC-type [Bacillus
UniRef100_P13267

Bacillus subtilis

PolC




subtilis]



1781


1782
Cellulose 1,4-beta-cellobiosidase
UniRef100_Q8KKF7

Paenibacillus sp. BP-23




precursor [Paenibacillus sp. BP-23]


1783
Endoglucanase B precursor
UniRef100_P23550

Paenibacillus lautus




[Paenibacillus lautus]


1784
Beta-mannosidase [Thermotoga
UniRef100_Q9RIK7

Thermotoga neapolitana





neapolitana]



1785
Hypothetical UPF0090 protein ylxS
UniRef100_P32726

Bacillus subtilis

YlxS



[Bacillus subtilis]


1786
Transcription elongation protein nusA
UniRef100_P32727

Bacillus subtilis

NusA



[Bacillus subtilis]


1787
Hypothetical protein ylxR [Bacillus
UniRef100_P32728

Bacillus subtilis





subtilis]



1788
Probable ribosomal protein ylxQ
UniRef100_P32729

Bacillus subtilis




[Bacillus subtilis]


1789
Translation initiation factor IF-2
UniRef100_P17889

Bacillus subtilis

InfB



[Bacillus subtilis]


1790
Hypothetical protein ylxP [Bacillus
UniRef100_P32730

Bacillus subtilis





subtilis]



1791
Ribosome-binding factor A [Bacillus
UniRef100_P32731

Bacillus subtilis

RbfA




subtilis]



1792



TruB


1793
Riboflavin biosynthesis protein ribC
UniRef100_P54575
Includes:
RibC



[Includes: Riboflavin kinase (EC

Riboflavin kinase



2.7.1.26) (Flavokinase); FMN

(EC 2.7.1.26)



adenylyltransferase (EC 2.7.7.2) (FAD

(Flavokinase);



pyrophosphorylase) (FAD synthetase)]

FMN



[Bacillus subtilis]

adenylyltransferase





(EC 2.7.7.2)





(FAD





pyrophosphorylase)





(FAD





synthetase)


1794
30S ribosomal protein S15 [Bacillus
UniRef100_P21473

Bacillus subtilis





subtilis]



1795
Polyribonucleotide
UniRef100_P50849

Bacillus subtilis

PnpA



nucleotidyltransferase [Bacillus subtilis]


1796
Hypothetical protein ylxY precursor
UniRef100_P50850

Bacillus subtilis

YlxY



[Bacillus subtilis]


1797
Hypothetical zinc protease ymxG
UniRef100_Q04805

Bacillus subtilis

MlpA



[Bacillus subtilis]


1798
Hypothetical protein ymxH [Bacillus
UniRef100_Q04811

Bacillus subtilis





subtilis]



1799
Dipicolinate synthase, A chain [Bacillus
UniRef100_Q04809

Bacillus subtilis

SpoVFA




subtilis]



1800
Dipicolinate synthase, B chain [Bacillus
UniRef100_Q04810

Bacillus subtilis

SpoVFB




subtilis]



1801
Aspartate-semialdehyde
UniRef100_Q04797

Bacillus subtilis

Asd



dehydrogenase [Bacillus subtilis]


1802
Aspartokinase 1 (EC 2.7.2.4)
UniRef100_Q04795
Contains:
DapG



(Aspartokinase I) (Aspartate kinase 1)

Aspartokinase I



[Contains: Aspartokinase I alpha

alpha subunit;



subunit; Aspartokinase I beta subunit]

Aspartokinase I



[Bacillus subtilis]

beta subunit


1803
Dihydrodipicolinate synthase [Bacillus
UniRef100_Q04796

Bacillus subtilis

DapA




subtilis]



1804
YmfA protein [Bacillus subtilis]
UniRef100_O31760

Bacillus subtilis

YmfA


1805
Translocation-enhancing protein tepA
UniRef100_Q99171

Bacillus subtilis

TepA



[Bacillus subtilis]


1806


1807
DNA translocase ftsK [Bacillus subtilis]
UniRef100_P21458

Bacillus subtilis

SpoIIIE


1808
Hypothetical transcriptional regulator
UniRef100_O31761

Bacillus subtilis

YmfC



ymfC [Bacillus subtilis]


1809
Multidrug resistance protein [Bacillus
UniRef100_Q9K7Q2

Bacillus

YitG




halodurans]



halodurans



1810
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81WP6

Bacillus

YmfG






anthracis



1811
YmfH protein [Bacillus subtilis]
UniRef100_O31766

Bacillus subtilis

YmfH


1812


1813


1814
YmfJ protein [Bacillus subtilis]
UniRef100_O31768

Bacillus subtilis



1815
Hypothetical protein [Bacillus cereus
UniRef100_Q636P2

Bacillus cereus

YmfK



ZK]

ZK


1816



YmfM


1817
CDP-diacylglycerol--glycerol-3-
UniRef100_P46322

Bacillus subtilis

PgsA



phosphate 3-phosphatidyltransferase



[Bacillus subtilis]


1818
CinA-like protein [Bacillus subtilis]
UniRef100_P46323

Bacillus subtilis

CinA


1819
RecA protein [Bacillus
UniRef100_Q8GJG2

Bacillus

RecA




amyloliquefaciens]



amyloliquefaciens



1820
Hypothetical UPF0144 protein ymdA
UniRef100_O31774

Bacillus subtilis

YmdA



[Bacillus subtilis]


1821
YmdB protein [Bacillus subtilis]
UniRef100_O31775

Bacillus subtilis

YmdB


1822
Stage V sporulation protein S [Bacillus
UniRef100_P45693

Bacillus subtilis





subtilis]



1823


1824


1825
L-threonine 3-dehydrogenase [Bacillus
UniRef100_O31776

Bacillus subtilis

Tdh




subtilis]



1826
2-amino-3-ketobutyrate coenzyme A
UniRef100_O31777

Bacillus subtilis

Kbl



ligase [Bacillus subtilis]


1827
Hypothetical UPF0004 protein ymcB
UniRef100_O31778

Bacillus subtilis

YmcB



[Bacillus subtilis]


1828
YmcA protein [Bacillus subtilis]
UniRef100_O31779

Bacillus subtilis

YmcA


1829
Spore coat protein E [Bacillus subtilis]
UniRef100_P14016

Bacillus subtilis

CotE


1830



MutS


1831
DNA mismatch repair protein mutL
UniRef100_P49850

Bacillus subtilis

MutL



[Bacillus subtilis]


1832
YjcS protein [Bacillus subtilis]
UniRef100_O31641

Bacillus subtilis



1833



YxiD


1834


1835


1836
All1751 protein [Anabaena sp.]
UniRef100_Q8YW65

Anabaena sp.

YciC


1837
BH0367 protein [Bacillus halodurans]
UniRef100_Q9KFV4

Bacillus halodurans



1838
Phosphinothricin N-acetyltransferase
UniRef100_Q9KFP5

Bacillus

YwnH



[Bacillus halodurans]


halodurans



1839
UPI00003CC0D8 UniRef100 entry
UniRef100_UPI00003CC0D8


1840


1841
Putative L-amino acid oxidase [Bacillus
UniRef100_O34363

Bacillus subtilis

YobN




subtilis]



1842



YoaK


1843
Na+/H+ antiporter [Bacillus halodurans]
UniRef100_Q9K5Q0

Bacillus

YvgP






halodurans



1844
Aromatic hydrocarbon catabolism
UniRef100_Q8CV32

Oceanobacillus iheyensis




protein [Oceanobacillus iheyensis]


1845
Hypothetical UPF0145 protein VP1283
UniRef100_Q87Q67

Vibrio parahaemolyticus




[Vibrio parahaemolyticus]


1846
Hypothetical protein yqeD [Bacillus
UniRef100_P54449

Bacillus subtilis

YqeD




subtilis]



1847
Penicillin-binding protein, putative
UniRef100_Q738U9

Bacillus cereus

PbpE



[Bacillus cereus]


1848
Hypothetical glycosyl transferase
UniRef100_O34539

Bacillus subtilis

YjiC



[Bacillus subtilis]


1849
Asparate-proton symporter [Bacillus
UniRef100_O07002

Bacillus subtilis

YveA




subtilis]



1850
Spore coat protein [Bacillus halodurans]
UniRef100_Q9KEV6

Bacillus halodurans



1851


1852



YdhD


1853
Putative HTH-type transcriptional
UniRef100_Q7WY76

Bacillus subtilis

YezE



regulator yezE [Bacillus subtilis]


1854
Hypothetical protein yesE [Bacillus
UniRef100_O31511

Bacillus subtilis

YesE




subtilis]



1855
YesF protein [Bacillus subtilis]
UniRef100_O31512

Bacillus subtilis

YesF


1856
UPI00003CBA3B UniRef100 entry
UniRef100_UPI00003CBA3B


1857
YmaD protein [Bacillus subtilis]
UniRef100_O31790

Bacillus subtilis

YmaD


1858
Multidrug resistance protein ebrB
UniRef100_O31791

Bacillus subtilis

EbrB



[Bacillus subtilis]


1859
Multidrug resistance protein ebrA
UniRef100_O31792

Bacillus subtilis

EbrA



[Bacillus subtilis]


1860


1861
Hypothetical protein ymaF [Bacillus
UniRef100_O31794

Bacillus subtilis

YmaF




subtilis]



1862
tRNA delta(2)-
UniRef100_O31795

Bacillus subtilis

MiaA



isopentenylpyrophosphate transferase



[Bacillus subtilis]


1863
Hfq protein [Bacillus subtilis]
UniRef100_O31796

Bacillus subtilis



1864
Hypothetical protein ymzA [Bacillus
UniRef100_O31798

Bacillus subtilis





subtilis]



1865
NrdI protein [Bacillus subtilis]
UniRef100_P50618

Bacillus subtilis

YmaA


1866
Ribonucleoside-diphosphate reductase
UniRef100_P50620

Bacillus subtilis

NrdE



alpha chain [Bacillus subtilis]


1867
Ribonucleoside-diphosphate reductase
UniRef100_P50621

Bacillus subtilis

NrdF



beta chain [Bacillus subtilis]


1868
Hypothetical protein ymaB [Bacillus
UniRef100_P50619

Bacillus subtilis

YmaB




subtilis]



1869
Blr6966 protein [Bradyrhizobium
UniRef100_Q89EV4

Bradyrhizobium

YtnP




japonicum]



japonicum



1870
Nitrogen fixation protein [Bacillus
UniRef100_Q9KFV2

Bacillus

YurV




halodurans]



halodurans



1871
Transcription regulator Fur family-like
UniRef100_Q8CNQ7

Staphylococcus

PerR



protein [Staphylococcus epidermidis]


epidermidis



1872
UPI00003CB681 UniRef100 entry
UniRef100_UPI00003CB681

YdhC


1873
Hypothetical protein [Bacillus cereus]
UniRef100_Q81C60

Bacillus cereus

YjlA


1874



CwlC


1875
Membrane protein, putative [Listeria
UniRef100_Q720L9

Listeria monocytogenes





monocytogenes]



1876
Lin1174 protein [Listeria innocua]
UniRef100_Q92CJ7

Listeria innocua



1877


1878


1879
Transcriptional regulator [Aquifex
UniRef100_O66635

Aquifex aeolicus

YdgC




aeolicus]



1880
Hypothetical membrane-anchored
UniRef100_Q92VA1

Rhizobium meliloti




protein [Rhizobium meliloti]


1881
Cytosine permease [Bacillus
UniRef100_Q9KBP3

Bacillus

YxlA




halodurans]



halodurans



1882
AgaF [Agrobacterium tumefaciens]
UniRef100_O50265

Agrobacterium tumefaciens



1883


1884
Hydantoin utilization protein B
UniRef100_Q88H51

Pseudomonas putida




[Pseudomonas putida]


1885
Hypothetical protein SMb20139
UniRef100_Q92X23

Rhizobium meliloti




[Rhizobium meliloti]


1886
BH2340 protein [Bacillus halodurans]
UniRef100_Q9KAE7

Bacillus halodurans



1887


1888


1889
Stage V sporulation protein K [Bacillus
UniRef100_P27643

Bacillus subtilis

SpoVK




subtilis]



1890
YnbA [Bacillus subtilis]
UniRef100_P94478

Bacillus subtilis

YnbA


1891
YnbB [Bacillus subtilis]
UniRef100_P94479

Bacillus subtilis

YnbB


1892
HTH-type transcriptional regulator glnR
UniRef100_P37582

Bacillus subtilis

GlnR



[Bacillus subtilis]


1893
Glutamine synthetase [Bacillus subtilis]
UniRef100_P12425

Bacillus subtilis

GlnA


1894


1895
Hypothetical protein CAC3435
UniRef100_Q97DN7

Clostridium acetobutylicum




[Clostridium acetobutylicum]


1896


1897


1898
Hypothetical protein CAC0350
UniRef100_Q97M50

Clostridium acetobutylicum




[Clostridium acetobutylicum]


1899
Hypothetical HIT-like protein MJ0866
UniRef100_Q58276

Methanococcus

Hit



[Methanococcus jannaschii]


jannaschii



1900
Hypothetical protein [Bacillus anthracis]
UniRef100_Q6I2B3

Bacillus anthracis



1901
Hypothetical protein [Bacillus cereus]
UniRef100_Q81CM1

Bacillus cereus

YdjC


1902


1903
Methyltransferase [Bacillus cereus]
UniRef100_Q81CJ2

Bacillus cereus



1904



YoaO


1905
Acetyltransferase, GNAT family
UniRef100_Q737B4

Bacillus cereus




[Bacillus cereus]


1906
Repressor rok [Bacillus subtilis]
UniRef100_O34857

Bacillus subtilis

Rok


1907


1908
Hypothetical protein [Bacteriophage T5]
UniRef100_Q6QGK2
Bacteriophage T5


1909
Hypothetical protein yvdT [Bacillus
UniRef100_O07001

Bacillus subtilis

YvdT




subtilis]



1910
Hypothetical protein yvdS [Bacillus
UniRef100_O32262

Bacillus subtilis

YvdS




subtilis]



1911
Hypothetical protein yvdR [Bacillus
UniRef100_O06999

Bacillus subtilis

YvdR




subtilis]



1912
Spermidine N1-acetyltransferase
UniRef100_Q72Y03

Bacillus cereus

YoaA



[Bacillus cereus]


1913
Hypothetical Membrane Associated
UniRef100_Q812L6

Bacillus cereus




Protein [Bacillus cereus]


1914
Hypothetical protein yoaW precursor
UniRef100_O34541

Bacillus subtilis

YoaW



[Bacillus subtilis]


1915
Thiol-disulfide oxidoreductase resA
UniRef100_Q9KCJ4

Bacillus

ResA



[Bacillus halodurans]


halodurans



1916
Manganese-containing catalase
UniRef100_Q9KAU6

Bacillus

YdbD



[Bacillus halodurans]


halodurans



1917
BH1562 protein [Bacillus halodurans]
UniRef100_Q9KCK9

Bacillus halodurans



1918
Acetyltransferase, GNAT family
UniRef100_Q739K0

Bacillus cereus

YjcK



[Bacillus cereus]


1919
Hypothetical conserved protein
UniRef100_Q8ELR7

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


1920


1921
Transcriptional regulator, MarR family
UniRef100_Q81BM5

Bacillus cereus

YkvE



[Bacillus cereus]


1922
Putative NAD(P)H nitroreductase ydfN
UniRef100_P96692

Bacillus subtilis

YdfN



[Bacillus subtilis]


1923
YdfO protein [Bacillus subtilis]
UniRef100_P96693

Bacillus subtilis

YdfO


1924
Hypothetical protein [Bacillus cereus
UniRef100_Q630S7

Bacillus cereus

YwrF



ZK]

ZK


1925
BH1010 protein [Bacillus halodurans]
UniRef100_Q9KE48

Bacillus

YhcB






halodurans



1926
ORF28 [Staphylococcus phage K]
UniRef100_Q6Y7T8

Staphylococcus phage K



1927


1928
Thymidylate synthase [Bacteriophage
UniRef100_P07606
Bacteriophage
ThyA



phi-3T]

phi-3T


1929
Sporulation-specific extracellular
UniRef100_P42983

Bacillus subtilis

NucB



nuclease precursor [Bacillus subtilis]


1930
LexA repressor [Bacillus subtilis]
UniRef100_P31080

Bacillus subtilis

LexA


1931
YneA [Bacillus subtilis]
UniRef100_Q45056

Bacillus subtilis

YneA


1932
YneB [Bacillus subtilis]
UniRef100_Q45057

Bacillus subtilis

YneB


1933
Hypothetical UPF0291 protein ynzC
UniRef100_O31818

Bacillus subtilis




[Bacillus subtilis]


1934
Transketolase [Bacillus subtilis]
UniRef100_P45694

Bacillus subtilis

Tkt


1935
Hypothetical protein yneE [Bacillus
UniRef100_P45707

Bacillus subtilis

YneE




subtilis]



1936
Hypothetical UPF0154 protein yneF
UniRef100_P45708

Bacillus subtilis




[Bacillus subtilis]


1937


1938
Cytochrome c-type biogenesis protein
UniRef100_P45706

Bacillus subtilis

CcdA



ccdA [Bacillus subtilis]


1939
CcdB protein [Bacillus subtilis]
UniRef100_P45709

Bacillus subtilis

YneI


1940
CcdC protein [Bacillus subtilis]
UniRef100_P45710

Bacillus subtilis

YneJ


1941
Hypothetical protein yneK [Bacillus
UniRef100_P45711

Bacillus subtilis

YneK




subtilis]



1942
Spore coat protein M [Bacillus subtilis]
UniRef100_Q45058

Bacillus subtilis

CotM


1943


1944


1945



CitB


1946
YneN protein [Bacillus subtilis]
UniRef100_O31820

Bacillus subtilis

YneN


1947


1948


1949
Small, acid-soluble spore protein tlp
UniRef100_Q45060

Bacillus subtilis




[Bacillus subtilis]


1950
YneP [Bacillus subtilis]
UniRef100_Q45061

Bacillus subtilis

YneP


1951
YneQ [Bacillus subtilis]
UniRef100_Q45062

Bacillus subtilis



1952
Hypothetical protein [Bacillus cereus]
UniRef100_Q815P1

Bacillus cereus



1953
Conserved domain protein [Bacillus
UniRef100_Q72YR7

Bacillus cereus





cereus]



1954
YneR [Bacillus subtilis]
UniRef100_Q45063

Bacillus subtilis



1955
Hypothetical UPF0078 protein yneS
UniRef100_Q45064

Bacillus subtilis

YneS



[Bacillus subtilis]


1956
YneT [Bacillus subtilis]
UniRef100_Q45065

Bacillus subtilis

YneT


1957
Topoisomerase IV subunit B [Bacillus
UniRef100_Q59192

Bacillus subtilis

ParE




subtilis]



1958
Topoisomerase IV subunit A [Bacillus
UniRef100_Q45066

Bacillus subtilis

ParC




subtilis]



1959



AraR


1960
Hypothetical conserved protein
UniRef100_Q8EMP2

Oceanobacillus

XylB



[Oceanobacillus iheyensis]


iheyensis



1961
L-ribulose-5-phosphate 4-epimerase
UniRef100_Q8EMP3

Oceanobacillus

AraD



[Oceanobacillus iheyensis]


iheyensis



1962
L-arabinose isomerase [Oceanobacillus
UniRef100_Q8EMP4

Oceanobacillus

AraA




iheyensis]



iheyensis



1963



YwtG


1964



FabG


1965
Hypothetical protein ynfC [Bacillus
UniRef100_Q45067

Bacillus subtilis

YnfC




subtilis]



1966
Amino acid carrier protein alsT [Bacillus
UniRef100_Q45068

Bacillus subtilis

AlsT




subtilis]



1967



NarI


1968



NarJ


1969



NarH


1970



NarG


1971


1972
Hypothetical protein yqfB [Lactococcus
UniRef100_Q9CF70

Lactococcus lactis





lactis]



1973



AlbA


1974



ArfM


1975



YwiC


1976
Transcriptional regulator of anaerobic
UniRef100_Q9KG81

Bacillus

Fnr



genes [Bacillus halodurans]


halodurans



1977
Nitrite extrusion protein [Bacillus
UniRef100_P46907

Bacillus subtilis

NarK




subtilis]



1978


1979
CAMP-binding domains-Catabolite
UniRef100_Q8R5P4

Thermoanaerobacter

Fnr



gene activator and regulatory subunit of


tengcongensis




cAMP-dependent protein kinases



[Thermoanaerobacter tengcongensis]


1980
Putative nitric oxide reductase
UniRef100_Q6GK48

Staphylococcus aureus




[Staphylococcus aureus]


1981


1982



YngL


1983



BglC


1984
Hypothetical protein ynfE [Bacillus
UniRef100_Q45069

Bacillus subtilis





subtilis]



1985
Hypothetical protein [Bacillus
UniRef100_Q9ZF48

Bacillus

YkkB




megaterium]



megaterium



1986
Hypothetical protein [Bacillus
UniRef100_Q70K06

Bacillus amyloliquefaciens





amyloliquefaciens]



1987
Alkyl hydroperoxide reductase large
UniRef100_Q9Z9W3

Bacillus

AhpF



subunit [Bacillus halodurans]


halodurans



1988
Methyltransferase [Methanosarcina
UniRef100_Q8PU82

Methanosarcina mazei





mazei]



1989
Similar to B. subtilis ywgB gene [Bacillus
UniRef100_Q9Z9W2

Bacillus

YwgB




halodurans]



halodurans



1990
Hypothetical protein ywoF [Bacillus
UniRef100_P94576

Bacillus subtilis

YwoF




subtilis]



1991
Branched-chain amino acid transport
UniRef100_P94499

Bacillus subtilis

BrnQ



system carrier protein brnQ [Bacillus




subtilis]



1992
NADP-dependent alcohol
UniRef100_O06007

Bacillus subtilis

AdhA



dehydrogenase [Bacillus subtilis]


1993
Transcriptional regulator, MerR family
UniRef100_Q721Z3

Listeria

YraB



[Listeria monocytogenes]


monocytogenes



1994
HPr-like protein crh [Bacillus subtilis]
UniRef100_O06976

Bacillus subtilis



1995
BH2089 protein [Bacillus halodurans]
UniRef100_Q9Z9R4

Bacillus

YddR






halodurans



1996


1997
Enoyl-CoA hydratase/isomerase family
UniRef100_Q738L0

Bacillus cereus

YngF



protein [Bacillus cereus]


1998
Hypothetical protein ysiB [Bacillus
UniRef100_P94549

Bacillus subtilis

YsiB




subtilis]



1999
Methylmalonic acid semialdehyde
UniRef100_Q63BL0

Bacillus cereus

MmsA



dehydrogenase [Bacillus cereus ZK]

ZK


2000
3-hydroxyisobutyrate dehydrogenase
UniRef100_Q63BL1

Bacillus cereus

YkwC



[Bacillus cereus ZK]

ZK


2001
Acyl-CoA dehydrogenase [Bacillus
UniRef100_Q81DR7

Bacillus cereus

YusJ




cereus]



2002
Mannose-6-phosphate isomerase
UniRef100_O31646

Bacillus subtilis

ManA



[Bacillus subtilis]


2003
Phosphotransferase system (PTS)
UniRef100_O31645

Bacillus subtilis

ManP



mannose-specific enzyme IIBCA



component [Bacillus subtilis]


2004


2005


2006
Hypothetical protein [Bacillus cereus]
UniRef100_Q72YT6

Bacillus cereus



2007
Transcriptional regulator [Bacillus
UniRef100_O31644

Bacillus subtilis

ManR




subtilis]



2008


2009
UPI00003CC220 UniRef100 entry
UniRef100_UPI00003CC220

YtrB


2010
Transcriptional regulator [Bacillus
UniRef100_Q9KF35

Bacillus

YtrA




halodurans]



halodurans



2011
Probable oxidoreductase [Clostridium
UniRef100_Q8XP17

Clostridium

YjmF




perfringens]



perfringens



2012
Mannonate dehydratase [Clostridium
UniRef100_Q8XP15

Clostridium

UxuA




perfringens]



perfringens



2013
Glucosidase [Bacillus halodurans]
UniRef100_Q9KEZ5

Bacillus halodurans



2014
C4-dicarboxylate transport system
UniRef100_Q9KEZ6

Bacillus halodurans




[Bacillus halodurans]


2015
C4-dicarboxylate transport system
UniRef100_Q8EMM5

Oceanobacillus iheyensis




permease small protein



[Oceanobacillus iheyensis]


2016
C4-dicarboxylate transport system
UniRef100_Q9KEZ8

Bacillus

DctB



[Bacillus halodurans]


halodurans



2017
Transcriptional regulator
UniRef100_Q8EL22

Oceanobacillus

CcpA



[Oceanobacillus iheyensis]


iheyensis



2018
Arsenate reductase [Bacillus subtilis]
UniRef100_P45947

Bacillus subtilis

ArsC


2019
YdfA protein [Bacillus subtilis]
UniRef100_P96678

Bacillus subtilis

YdfA


2020
YdeT protein [Bacillus subtilis]
UniRef100_P96677

Bacillus subtilis

YdeT


2021
YdeI [Bacillus halodurans]
UniRef100_Q9Z9R5

Bacillus

YdeI






halodurans



2022
Putative secreted protein
UniRef100_Q9S1Z5

Streptomyces coelicolor




[Streptomyces coelicolor]


2023
Probable glucose uptake protein glcU
UniRef100_P40419

Bacillus

GlcU



[Bacillus megaterium]


megaterium



2024
YngK protein [Bacillus subtilis]
UniRef100_O35015

Bacillus subtilis

YngK


2025
YngD protein [Bacillus subtilis]
UniRef100_O31824

Bacillus subtilis

YngD


2026
Pyruvate formate-lyase-activating
UniRef100_Q73DZ6

Bacillus cereus

YkvL



enzyme [Bacillus cereus]


2027
Formate acetyltransferase [Bacillus
UniRef100_Q81YX1

Bacillus anthracis





anthracis]



2028



DacC


2029
NADH dehydrogenase-like protein yjlD
UniRef100_P80861

Bacillus subtilis

YjlD



[Bacillus subtilis]


2030
Hypothetical protein yjlC [Bacillus
UniRef100_O34633

Bacillus subtilis

YjlC




subtilis]



2031


2032
Hypothetical protein [Bacillus cereus]
UniRef100_Q81IJ8

Bacillus cereus



2033
Hypothetical protein ykzH [Bacillus
UniRef100_O31653

Bacillus subtilis





subtilis]



2034
Acetyl-CoA synthetase [Bacillus
UniRef100_Q9KDS4

Bacillus

AcsA



halodurans]


halodurans



2035
YngE protein [Bacillus subtilis]
UniRef100_O31825

Bacillus subtilis

YngE


2036
Hydroxybutyryl-dehydratase [Bacillus
UniRef100_Q9L7W1

Bacillus subtilis

YngF




subtilis]



2037
YngG protein [Bacillus subtilis]
UniRef100_O34873

Bacillus subtilis

YngG


2038
YngXX [Bacillus subtilis]
UniRef100_Q9R9I3

Bacillus subtilis



2039
YngH [Bacillus subtilis]
UniRef100_Q9R9I4

Bacillus subtilis

YngH


2040
YngI [Bacillus subtilis]
UniRef100_Q9R9I5

Bacillus subtilis

YngI


2041
YngJ protein [Bacillus subtilis]
UniRef100_O34421

Bacillus subtilis

YngJ


2042
NAD(P)H oxidoreductase YRKL
UniRef100_Q7P6P0

Fusobacterium

YrkL



[Fusobacterium nucleatum subsp.


nucleatum





vincentii ATCC 49256]


subsp. vincentii





ATCC 49256


2043
Transcriptional regulator, MarR family
UniRef100_Q8RE85

Fusobacterium nucleatum




[Fusobacterium nucleatum]


2044
Glutamate-5-semialdehyde
UniRef100_Q6HHC2

Bacillus

ProA



dehydrogenase [Bacillus thuringiensis]


thuringiensis



2045
Glutamate 5-kinase 2 [Bacillus subtilis]
UniRef100_O07509

Bacillus subtilis

ProJ


2046
Pyrroline-5-carboxylate reductase 1
UniRef100_P14383

Bacillus subtilis

ProH



[Bacillus subtilis]


2047
UPI00003CB6CD UniRef100 entry
UniRef100_UPI00003CB6CD

YetF


2048
Sodium-dependent phosphate
UniRef100_Q9KCT1

Bacillus

CysP



transporter [Bacillus halodurans]


halodurans



2049
Probable phosphoadenosine
UniRef100_O06737

Bacillus subtilis

YitB



phosphosulfate reductase [Bacillus




subtilis]



2050
Phosphosulfolactate synthase (EC
UniRef100_O06739

Bacillus subtilis

YitD



4.4.1.19) ((2R)-phospho-3-sulfolactate



synthase) [Bacillus subtilis]


2051
YitE [Bacillus subtilis]
UniRef100_O06740

Bacillus subtilis

YitE


2052
YitF [Bacillus subtilis]
UniRef100_O06741

Bacillus subtilis

YitF


2053
YitG [Bacillus subtilis]
UniRef100_O06742

Bacillus subtilis

YitG


2054
Putative glycosyl transferase ykoT
UniRef100_O34755

Bacillus subtilis

YkoT



[Bacillus subtilis]


2055
YkoR [Bacillus subtilis]
UniRef100_O34830

Bacillus subtilis

YkoS


2056
Glutamate synthase [NADPH] small
UniRef100_O34399
NADPH
GltB



chain [Bacillus subtilis]


2057



GltA


2058
HTH-type transcriptional regulator gltC
UniRef100_P20668

Bacillus subtilis

GltC



[Bacillus subtilis]


2059
AI17121 protein [Anabaena sp.]
UniRef100_Q8YL17

Anabaena sp.



2060


2061
Lmo0606 protein [Listeria
UniRef100_Q8Y9C6

Listeria monocytogenes





monocytogenes]



2062
ABC transporter ATP-binding protein
UniRef100_Q67MU2

Symbiobacterium

YfiB



[Symbiobacterium thermophilum]


thermophilum



2063
ABC transporter ATP-binding protein
UniRef100_Q67MU3

Symbiobacterium

YfiC



[Symbiobacterium thermophilum]


thermophilum



2064
Cytochrome P450 109 [Bacillus subtilis]
UniRef100_P27632

Bacillus subtilis

YjiB


2065
Hypothetical oxidoreductase yoxD
UniRef100_P14802

Bacillus subtilis

YoxD



[Bacillus subtilis]


2066



Pps


2067
Permease, general substrate
UniRef100_Q6HMC3

Bacillus

LmrB



transporter [Bacillus thuringiensis]


thuringiensis



2068
Putative formate dehydrogenase
UniRef100_O34323

Bacillus subtilis

YoaE



[Bacillus subtilis]


2069
Transcriptional regulatory protein
UniRef100_Q89KD1

Bradyrhizobium

YcgE



[Bradyrhizobium japonicum]


japonicum



2070
Drug resistance transporter,
UniRef100_Q73615

Bacillus cereus

Mdr



EmrB/QacA family [Bacillus cereus]


2071
YndE protein [Bacillus subtilis]
UniRef100_O31809

Bacillus subtilis

YndE


2072
YndF protein [Bacillus subtilis]
UniRef100_O31810

Bacillus subtilis

YndF


2073



YndE


2074



YndE


2075
UPI00003CB22E UniRef100 entry
UniRef100_UPI00003CB22E

YndD


2076


2077
Nucleotide binding protein expZ
UniRef100_P39115

Bacillus subtilis

ExpZ



[Bacillus subtilis]


2078



TlpB


2079
CsaA protein [Bacillus subtilis]
UniRef100_P37584

Bacillus subtilis

CsaA


2080
Alkaline serine protease [Bacillus
UniRef100_O31788

Bacillus subtilis

AprX




subtilis]



2081
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81SD4

Bacillus

YnzE






anthracis



2082
Transcriptional regulator, TetR family
UniRef100_Q63D70

Bacillus cereus

YrhI



[Bacillus cereus ZK]

ZK


2083
Putative HTH-type transcriptional
UniRef100_P40762

Bacillus subtilis

YvmB



regulator yvmB [Bacillus subtilis]


2084
Hydrolase [Bacillus cereus]
UniRef100_Q81D79

Bacillus cereus

YqeK


2085
Hypothetical conserved protein
UniRef100_Q8ETG3

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


2086
YndJ protein [Bacillus subtilis]
UniRef100_O31813

Bacillus subtilis

YndJ


2087
YndH protein [Bacillus subtilis]
UniRef100_O31812

Bacillus subtilis

YndH


2088
Hypothetical protein [Bacillus cereus]
UniRef100_Q73A97

Bacillus cereus

YndG


2089
UPI00003CBA97 UniRef100 entry
UniRef100_UPI00003CBA97

YobS


2090
UPI00003CBA98 UniRef100 entry
UniRef100_UPI00003CBA98

YobT


2091
DNA-binding protein YobU [Bacillus
UniRef100_O34637

Bacillus subtilis

YobU




subtilis]



2092
Hypothetical protein [Bacteroides
UniRef100_Q64RP1

Bacteroides fragilis





fragilis]



2093
Transcription regulator [Bacillus subtilis]
UniRef100_O34920

Bacillus subtilis

YobV


2094



YobW


2095
YozA protein [Bacillus subtilis]
UniRef100_O31844

Bacillus subtilis



2096
Possible metallo-beta-lactamase family
UniRef100_Q638G1

Bacillus cereus

YmaE



protein [Bacillus cereus ZK]

ZK


2097


2098



YfnA


2099



YocA


2100
UPI00003CBE6E UniRef100 entry
UniRef100_UPI00003CBE6E

YveM


2101
Hypothetical protein [Bacillus cereus]
UniRef100_Q817C2

Bacillus cereus



2102


2103
Glycosyl transferase [Bacillus
UniRef100_Q9K7I1

Bacillus

YtcC




halodurans]



halodurans



2104
YozB protein [Bacillus subtilis]
UniRef100_O31845

Bacillus subtilis

YozB


2105
Lmo2079 protein [Listeria
UniRef100_Q8Y5I3

Listeria monocytogenes





monocytogenes]



2106
YocC [Bacillus subtilis]
UniRef100_O35042

Bacillus subtilis

YocC


2107
Na+/myo-inositol cotransporter [Bacillus
UniRef100_Q9KAR5

Bacillus

YcgO




halodurans]



halodurans



2108
YocH [Bacillus subtilis]
UniRef100_O34669

Bacillus subtilis

YocH


2109
RecQ homolog [Bacillus subtilis]
UniRef100_O34748

Bacillus subtilis

YocI


2110
Hypothetical protein yqbC [Bacillus
UniRef100_P45919

Bacillus subtilis

YqbC




subtilis]



2111
Hypothetical protein yjfB [Bacillus
UniRef100_O34438

Bacillus subtilis





subtilis]



2112
Hypothetical protein yyaQ [Bacillus
UniRef100_P37507

Bacillus subtilis

YyaQ




subtilis]



2113
Hypothetical protein OB2103
UniRef100_Q8EPJ8

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


2114


2115
Hypothetical protein yjgD [Bacillus
UniRef100_O34681

Bacillus subtilis

YjgD




subtilis]



2116



YjgC


2117



YjgC


2118
Hypothetical protein OB3361
UniRef100_Q8EL70

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


2119
Hypothetical protein ypfA [Bacillus
UniRef100_P38491

Bacillus subtilis

YpfA




subtilis]



2120


2121


2122



YxiB


2123


2124


2125


2126



YndF


2127


2128
Hypothetical protein [Bacillus cereus]
UniRef100_Q816C2

Bacillus cereus



2129
BH0185 protein [Bacillus halodurans]
UniRef100_Q9KGB9

Bacillus halodurans



2130


2131



YisY


2132
Hypothetical protein yoqH
UniRef100_O64117
Bacteriophage
YoqH



[Bacteriophage SPBc2]

SPBc2


2133


2134
BH0429 protein [Bacillus halodurans]
UniRef100_Q9KFP9

Bacillus

YrhP






halodurans



2135
30S ribosomal protein S14
UniRef100_Q8ETX0

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


2136
UPI00002DEBB5 UniRef100 entry
UniRef100_UPI00002DEBB5

MutT


2137
Pyruvate water dikinase
UniRef100_Q8TN35

Methanosarcina

Pps



[Methanosarcina acetivorans]


acetivorans



2138
Transcriptional regulator
UniRef100_Q8ESJ8

Oceanobacillus

YxbF



[Oceanobacillus iheyensis]


iheyensis



2139


2140
YndM protein [Bacillus subtilis]
UniRef100_O31816

Bacillus subtilis

YndM


2141
Hypothetical protein yisT [Bacillus
UniRef100_O07939

Bacillus subtilis

YisT




subtilis]



2142
Putative acyl carrier protein
UniRef100_O35022

Bacillus subtilis

YocJ



phosphodiesterase 1 [Bacillus subtilis]


2143
General stress protein 16O [Bacillus
UniRef100_P80872

Bacillus subtilis

YocK




subtilis]



2144


2145


2146
Aldehyde dehydrogenase [Bacillus
UniRef100_O34660

Bacillus subtilis

DhaS




subtilis]



2147
YjbB protein [Bacillus subtilis]
UniRef100_O31600

Bacillus subtilis

YjbB


2148
Aminoglycoside N6′-acetyltransferase
UniRef100_Q81AT3

Bacillus cereus

YjcK



[Bacillus cereus]


2149
Blasticidin S deaminase, putative
UniRef100_Q81Y61

Bacillus anthracis




[Bacillus anthracis]


2150



SqhC


2151
Probable superoxide dismutase [Fe]
UniRef100_O35023
Fe
SodF



[Bacillus subtilis]


2152
Stress response protein yvgO precursor
UniRef100_O32211

Bacillus subtilis

YvgO



[Bacillus subtilis]


2153
Putative transporter [Bacillus subtilis]
UniRef100_O34383

Bacillus subtilis

YocR


2154
Putative transporter [Bacillus subtilis]
UniRef100_O34524

Bacillus subtilis

YocS


2155
Dihydrolipoyllysine-residue
UniRef100_P16263

Bacillus subtilis

OdhB



succinyltransferase component of 2-



oxoglutarate dehydrogenase complex



[Bacillus subtilis]


2156
2-oxoglutarate dehydrogenase E1
UniRef100_P23129

Bacillus subtilis

OdhA



component [Bacillus subtilis]


2157
YojO protein [Bacillus subtilis]
UniRef100_O31849

Bacillus subtilis

YojO


2158
YojN protein [Bacillus subtilis]
UniRef100_O31850

Bacillus subtilis

YojN


2159
Hypothetical superoxide dismutase-like
UniRef100_O31851

Bacillus subtilis

YojM



protein yojM precursor [Bacillus subtilis]


2160
Hypothetical protein yojL precursor
UniRef100_O31852

Bacillus subtilis

LytF



[Bacillus subtilis]


2161
Probable multidrug resistance protein
UniRef100_O31855

Bacillus subtilis

YojI



norM (Na(+)/drug antiporter) [Bacillus




subtilis]



2162
Hypothetical protein [Bacillus cereus
UniRef100_Q637Z8

Bacillus cereus

YojG



ZK]

ZK


2163
YojF protein [Bacillus subtilis]
UniRef100_O31858

Bacillus subtilis

YojF


2164
YojE [Bacillus subtilis]
UniRef100_O68260

Bacillus subtilis



2165
YojE protein [Bacillus subtilis]
UniRef100_O31859

Bacillus subtilis

YojE


2166
Hypothetical protein yozR [Bacillus
UniRef100_Q7WY67

Bacillus subtilis

YozR




subtilis]



2167
YoaJ [Bacillus subtilis]
UniRef100_O34918

Bacillus subtilis

YoaJ


2168


2169
UPI00003CBE9B UniRef100 entry
UniRef100_UPI00003CBE9B

YwfD


2170
Hypothetical UPF0087 protein yodB
UniRef100_O34844

Bacillus subtilis

YodB



[Bacillus subtilis]


2171
Putative NAD(P)H nitroreductase 12C
UniRef100_P81102

Bacillus subtilis

YodC



[Bacillus subtilis]


2172
YolF [Bacillus subtilis]
UniRef100_O34842

Bacillus subtilis

YodD


2173
UPI0000315ACC UniRef100 entry
UniRef100_UPI0000315ACC


2174
YodF protein [Bacillus subtilis]
UniRef100_O34745

Bacillus subtilis

YodF


2175
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012





anthracis str. A2012]



2176
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069


2177
OrfRM1 [Bacillus subtilis]
UniRef100_O34666

Bacillus subtilis

CtpA


2178
YolB [Bacillus subtilis]
UniRef100_O34954

Bacillus subtilis

YodH


2179


2180
Carboxypeptidase [Bacillus subtilis]
UniRef100_O34866

Bacillus subtilis

YodJ


2181
Purine nucleoside phosphorylase II
UniRef100_O34925

Bacillus subtilis

DeoD



[Bacillus subtilis]


2182
Hypothetical Membrane Spanning
UniRef100_Q813P0

Bacillus cereus

YcgR



Protein [Bacillus cereus]


2183
YcgQ protein [Bacillus subtilis]
UniRef100_P94394

Bacillus subtilis

YcgQ


2184


2185
Hypothetical protein yodL [Bacillus
UniRef100_O30472

Bacillus subtilis

YodL




subtilis]



2186



YodM


2187
Hypothetical protein yozD [Bacillus
UniRef100_O31863

Bacillus subtilis





subtilis]



2188
Hypothetical protein yodN [Bacillus
UniRef100_O34414

Bacillus subtilis

YodN




subtilis]



2189


2190
YokU [Bacillus subtilis]
UniRef100_O30470

Bacillus subtilis



2191
Hypothetical UPF0069 protein yodO
UniRef100_O34676

Bacillus subtilis

KamA



[Bacillus subtilis]


2192
YodP [Bacillus subtilis]
UniRef100_O34895

Bacillus subtilis

YodP


2193
Acetylornitine deacetylase [Bacillus
UniRef100_O34984

Bacillus subtilis

YodQ




subtilis]



2194
Butirate-acetoacetate CoA transferase
UniRef100_O34466

Bacillus subtilis

YodR



[Bacillus subtilis]


2195
Butyrate acetoacetate-CoA transferase
UniRef100_O34317

Bacillus subtilis

YodS



[Bacillus subtilis]


2196
Probable aminotransferase yodT
UniRef100_O34662

Bacillus subtilis

YodT



[Bacillus subtilis]


2197
Multidrug resistance protein; possible
UniRef100_Q6HK46

Bacillus

YkuC



tetracycline resistance determinant


thuringiensis




[Bacillus thuringiensis]


2198
Protein cgeE [Bacillus subtilis]
UniRef100_P42093

Bacillus subtilis

CgeE


2199
Peptide methionine sulfoxide reductase
UniRef100_P54155

Bacillus subtilis

YppQ



msrB [Bacillus subtilis]


2200



MsrA


2201
Putative HTH-type transcriptional
UniRef100_P54182

Bacillus subtilis

YpoP



regulator ypoP [Bacillus subtilis]


2202


2203
Hypothetical protein yhcK [Bacillus
UniRef100_P54595

Bacillus subtilis

YhcK




subtilis]



2204
Hypothetical protein ypnP [Bacillus
UniRef100_P54181

Bacillus subtilis

YpnP




subtilis]



2205
Hypothetical conserved protein
UniRef100_Q746K9

Thermus thermophilus




[Thermus thermophilus]


2206


2207
Hypothetical protein ypmS [Bacillus
UniRef100_P54179

Bacillus subtilis

YpmS




subtilis]



2208
Hypothetical protein ypmR [Bacillus
UniRef100_P40766

Bacillus subtilis

YpmR




subtilis]



2209
Hypothetical protein ypmQ [Bacillus
UniRef100_P54178

Bacillus subtilis

YpmQ




subtilis]



2210
DegV family protein [Bacillus cereus
UniRef100_Q63BU6

Bacillus cereus

YviA



ZK]

ZK


2211
Hypothetical protein ypmP [Bacillus
UniRef100_P54177

Bacillus subtilis





subtilis]



2212
Threonine dehydratase biosynthetic
UniRef100_P37946

Bacillus subtilis

IlvA



[Bacillus subtilis]


2213
Putative sigma L-dependent
UniRef100_P54156

Bacillus subtilis

YplP



transcriptional regulator yplP [Bacillus




subtilis]



2214
Hemolysin III homolog [Bacillus subtilis]
UniRef100_P54175

Bacillus subtilis

YplQ


2215
Hypothetical protein ypkP [Bacillus
UniRef100_P54174

Bacillus subtilis

YpkP




subtilis]



2216
Dihydrofolate reductase [Bacillus
UniRef100_P11045

Bacillus subtilis

DfrA




subtilis]



2217
Hypothetical protein ypjQ [Bacillus
UniRef100_P54173

Bacillus subtilis

YpjQ




subtilis]



2218



YpjP


2219



YpjP


2220
Hypothetical protein yphP [Bacillus
UniRef100_P54170

Bacillus subtilis

YphP




subtilis]



2221
Dihydroxy-acid dehydratase [Bacillus
UniRef100_P51785

Bacillus subtilis

IlvD




subtilis]



2222



YpgR


2223
Hypothetical protein ypgQ [Bacillus
UniRef100_P54168

Bacillus subtilis

YpgQ




subtilis]



2224
Glutathione peroxidase homolog bsaA
UniRef100_P52035

Bacillus subtilis

BsaA



[Bacillus subtilis]


2225
UPI00003CBA0F UniRef100 entry
UniRef100_UPI00003CBA0F


2226
Homoserine O-succinyltransferase
UniRef100_P54167

Bacillus subtilis

MetA



[Bacillus subtilis]


2227
Putative glycosyl transferase ypfP
UniRef100_P54166

Bacillus subtilis

UgtP



[Bacillus subtilis]


2228


2229
Cold shock protein cspD [Bacillus
UniRef100_P51777

Bacillus subtilis





subtilis]



2230
Regulatory protein degR [Bacillus
UniRef100_P06563

Bacillus subtilis





subtilis]



2231
Hypothetical protein ypzA [Bacillus
UniRef100_O32007

Bacillus subtilis





subtilis]



2232


2233
Hypothetical protein ypeP [Bacillus
UniRef100_P54164

Bacillus subtilis

YpeP




subtilis]



2234
Hypothetical protein ypdP [Bacillus
UniRef100_P54163

Bacillus subtilis

YpdP




subtilis]



2235
14.7 kDa ribonuclease H-like protein
UniRef100_P54162

Bacillus subtilis

YpdQ



[Bacillus subtilis]


2236
Probable 5′-3′ exonuclease [Bacillus
UniRef100_P54161

Bacillus subtilis

YpcP




subtilis]



2237


2238
Hypothetical protein ypbS [Bacillus
UniRef100_P54160

Bacillus subtilis





subtilis]



2239
Hypothetical protein ypbR [Bacillus
UniRef100_P54159

Bacillus subtilis

YpbR




subtilis]



2240


2241
Hypothetical protein ypbQ [Bacillus
UniRef100_P54158

Bacillus subtilis

YpbQ




subtilis]



2242



BcsA


2243
Predicted acetyltransferase [Clostridium
UniRef100_Q97G03

Clostridium

YokL




acetobutylicum]



acetobutylicum



2244
Xanthine permease [Bacillus subtilis]
UniRef100_P42086

Bacillus subtilis

PbuX


2245
Xanthine phosphoribosyltransferase
UniRef100_P42085

Bacillus subtilis

Xpt



[Bacillus subtilis]


2246
Hypothetical metalloprotease ypwA
UniRef100_P50848

Bacillus subtilis

YpwA



[Bacillus subtilis]


2247



YpvA


2248
Hypothetical protein yptA precursor
UniRef100_P50841

Bacillus subtilis




[Bacillus subtilis]


2249


2250
Hypothetical UPF0020 protein ypsC
UniRef100_P50840

Bacillus subtilis

YpsC



precursor [Bacillus subtilis]


2251
Hypothetical protein ypsB [Bacillus
UniRef100_P50839

Bacillus subtilis





subtilis]



2252
Hypothetical protein ypsA [Bacillus
UniRef100_P50838

Bacillus subtilis

YpsA




subtilis]



2253
Spore coat protein D [Bacillus subtilis]
UniRef100_P07791

Bacillus subtilis



2254


2255



YprB


2256



YprA


2257
Putative PTS system IIA component
UniRef100_P50829

Bacillus subtilis

YpqE



ypqE [Bacillus subtilis]


2258
Hypothetical protein ypqA precursor
UniRef100_P50836

Bacillus subtilis

YpqA



[Bacillus subtilis]


2259
Hypothetical protein yppG [Bacillus
UniRef100_P50835

Bacillus subtilis

YppG




subtilis]



2260


2261



YppE


2262


2263


2264
Hypothetical protein yppC [Bacillus
UniRef100_P39791

Bacillus subtilis

YppC




subtilis]



2265
Recombination protein U [Bacillus
UniRef100_P39792

Bacillus subtilis

RecU




subtilis]



2266
Penicillin-binding protein 1A/1B (PBP1)
UniRef100_P39793
Includes:
PonA



[Includes: Penicillin-insensitive

Penicillin-



transglycosylase (EC 2.4.2.—)

insensitive



(Peptidoglycan TGase); Penicillin-

transglycosylase



sensitive transpeptidase (EC 3.4.—.—)

(EC 2.4.2.—)



(DD-transpeptidase)] [Bacillus subtilis]

(Peptidoglycan





TGase);





Penicillin-





sensitive





transpeptidase





(EC 3.4.—.—) (DD-





transpeptidase)


2267
Hypothetical protein ypoC [Bacillus
UniRef100_P39789

Bacillus subtilis

YpoC




subtilis]



2268
Probable endonuclease III (EC
UniRef100_P39788

Bacillus subtilis

Nth



4.2.99.18) (DNA-(apurinic or



apyrimidinic site) lyase) [Bacillus




subtilis]



2269
DNA replication protein dnaD [Bacillus
UniRef100_P39787

Bacillus subtilis

DnaD




subtilis]



2270
Asparaginyl-tRNA synthetase [Bacillus
UniRef100_P39772

Bacillus subtilis

AsnS




subtilis]



2271
Aspartate aminotransferase [Bacillus
UniRef100_P53001

Bacillus subtilis

AspB




subtilis]



2272
Hypothetical protein ypmB [Bacillus
UniRef100_P54396

Bacillus subtilis

YpmB




subtilis]



2273
Hypothetical protein ypmA [Bacillus
UniRef100_P54395

Bacillus subtilis





subtilis]



2274
Probable ATP-dependent helicase dinG
UniRef100_P54394

Bacillus subtilis

DinG



homolog [Bacillus subtilis]


2275
Aspartate 1-decarboxylase precursor
UniRef100_P52999

Bacillus subtilis

PanD



[Bacillus subtilis]


2276
Pantoate--beta-alanine ligase [Bacillus
UniRef100_P52998

Bacillus subtilis

PanC




subtilis]



2277
3-methyl-2-oxobutanoate
UniRef100_P52996

Bacillus subtilis

PanB



hydroxymethyltransferase [Bacillus




subtilis]



2278
BirA bifunctional protein [Includes:
UniRef100_P42975
Includes: Biotin
BirA



Biotin operon repressor; Biotin--[acetyl-

operon



CoA-carboxylase] synthetase (EC

repressor; Biotin--



6.3.4.15) (Biotin--protein ligase)]



[Bacillus subtilis]


2279
Poly(A) polymerase [Bacillus subtilis]
UniRef100_P42977

Bacillus subtilis

Cca


2280
Putative glycosyl transferase ypjH
UniRef100_P42982

Bacillus subtilis

YpjH



[Bacillus subtilis]


2281
Hypothetical protein ypjG [Bacillus
UniRef100_P42981

Bacillus subtilis

YpjG




subtilis]



2282
Methylglyoxal synthase [Bacillus
UniRef100_P42980

Bacillus subtilis

MgsA




subtilis]



2283
Dihydrodipicolinate reductase [Bacillus
UniRef100_P42976

Bacillus subtilis

DapB




subtilis]



2284
Hypothetical protein ypjD [Bacillus
UniRef100_P42979

Bacillus subtilis

YpjD




subtilis]



2285



YpjC


2286
Hypothetical protein ypjB precursor
UniRef100_P54393

Bacillus subtilis

YpjB



[Bacillus subtilis]


2287



YpjA


2288



QcrC


2289
Menaquinol-cytochrome c reductase
UniRef100_P46912

Bacillus subtilis

QcrB



cytochrome b subunit [Bacillus subtilis]


2290
Menaquinol-cytochrome c reductase
UniRef100_P46911

Bacillus subtilis

QcrA



iron-sulfur subunit [Bacillus subtilis]


2291
Hypothetical protein ypiF [Bacillus
UniRef100_P54391

Bacillus subtilis

YpiF




subtilis]



2292
Hypothetical UPF0302 protein ypiB
UniRef100_P54390

Bacillus subtilis

YpiB



[Bacillus subtilis]


2293
Hypothetical protein ypiA [Bacillus
UniRef100_P54389

Bacillus subtilis

YpiA




subtilis]



2294
3-phosphoshikimate 1-
UniRef100_P20691

Bacillus subtilis

AroE



carboxyvinyltransferase [Bacillus




subtilis]



2295
Prephenate dehydrogenase [Bacillus
UniRef100_P20692

Bacillus subtilis

TyrA




subtilis]



2296



HisC


2297
Tryptophan synthase alpha chain
UniRef100_P07601

Bacillus subtilis

TrpA



[Bacillus subtilis]


2298
Tryptophan synthase beta chain
UniRef100_P07600

Bacillus subtilis

TrpB



[Bacillus subtilis]


2299
N-(5′-phosphoribosyl)anthranilate
UniRef100_P20167

Bacillus subtilis

TrpF



isomerase [Bacillus subtilis]


2300
Indole-3-glycerol phosphate synthase
UniRef100_P03964

Bacillus subtilis

TrpC



[Bacillus subtilis]


2301
Anthranilate phosphoribosyltransferase
UniRef100_P03947

Bacillus subtilis

TrpD



[Bacillus subtilis]


2302
Anthranilate synthase component I
UniRef100_P03963

Bacillus subtilis

TrpE



[Bacillus subtilis]


2303
Chorismate mutase [Bacillus subtilis]
UniRef100_P19080

Bacillus subtilis

AroH


2304
3-dehydroquinate synthase [Bacillus
UniRef100_P31102

Bacillus subtilis

AroB




subtilis]



2305
Chorismate synthase [Bacillus subtilis]
UniRef100_P31104

Bacillus subtilis

AroF


2306
Chemotaxis protein methyltransferase
UniRef100_P31105

Bacillus subtilis

CheR



[Bacillus subtilis]


2307
Nucleoside diphosphate kinase
UniRef100_P31103

Bacillus subtilis

Ndk



[Bacillus subtilis]


2308
Heptaprenyl diphosphate synthase
UniRef100_P31114

Bacillus subtilis

HepT



component II [Bacillus subtilis]


2309
Menaquinone biosynthesis
UniRef100_P31113

Bacillus subtilis

MenH



methyltransferase ubiE [Bacillus




subtilis]



2310
Heptaprenyl diphosphate synthase
UniRef100_P31112

Bacillus subtilis

HepS



component I [Bacillus subtilis]


2311
Transcription attenuation protein mtrB
UniRef100_P19466

Bacillus subtilis




[Bacillus subtilis]


2312
GTP cyclohydrolase I [Bacillus subtilis]
UniRef100_P19465

Bacillus subtilis

MtrA


2313
DNA-binding protein HU 1 [Bacillus
UniRef100_P08821

Bacillus subtilis





subtilis]



2314
Stage IV sporulation protein A [Bacillus
UniRef100_P35149

Bacillus subtilis

SpoIVA




subtilis]



2315
Hypothetical protein yphF [Bacillus
UniRef100_P39911

Bacillus subtilis

YphF




subtilis]



2316
Hypothetical protein yphE [Bacillus
UniRef100_P50744

Bacillus subtilis





subtilis]



2317
Glycerol-3-phosphate dehydrogenase
UniRef100_P46919
NAD(P)+
GpsA



[NAD(P)+] (EC 1.1.1.94) (NAD(P)H-



dependent glycerol-3-phosphate



dehydrogenase) (NAD(P)H-dependent



dihydroxyacetone-phosphate



reductase) [Bacillus subtilis]


2318



YphC


2319
Hypothetical protein OB1798
UniRef100_Q8EQA7

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


2320
Hypothetical protein yphB [Bacillus
UniRef100_P50742

Bacillus subtilis

SeaA




subtilis]



2321
Hypothetical protein yphA [Bacillus
UniRef100_P50741

Bacillus subtilis

YphA




subtilis]



2322


2323



YpgA


2324
30S ribosomal protein S1 homolog
UniRef100_P38494

Bacillus subtilis

YpfD



[Bacillus subtilis]


2325
Cytidylate kinase [Bacillus subtilis]
UniRef100_P38493

Bacillus subtilis

Cmk


2326
Hypothetical protein ypfB [Bacillus
UniRef100_P38492

Bacillus subtilis





subtilis]



2327
Sporulation protein ypeB [Bacillus
UniRef100_P38490

Bacillus subtilis

YpeB




subtilis]



2328
Spore cortex-lytic enzyme precursor
UniRef100_P50739

Bacillus subtilis

SleB



[Bacillus subtilis]


2329



YpdC


2330



YccC


2331
Hypothetical protein ypdA [Bacillus
UniRef100_P50736

Bacillus subtilis

YpdA




subtilis]



2332
NAD-specific glutamate dehydrogenase
UniRef100_P50735

Bacillus subtilis

GudB



[Bacillus subtilis]


2333
Adapter protein mecA 2 [Bacillus
UniRef100_P50734

Bacillus subtilis

YpbH




subtilis]



2334
Hypothetical protein ypbG precursor
UniRef100_P50733

Bacillus subtilis

YpbG



[Bacillus subtilis]


2335



YpbF


2336
Hypothetical protein ypbE [Bacillus
UniRef100_P50731

Bacillus subtilis

YpbE




subtilis]



2337
Hypothetical protein ypbD [Bacillus
UniRef100_P50730

Bacillus subtilis

YpbD




subtilis]



2338
ATP-dependent DNA helicase recQ
UniRef100_P50729

Bacillus subtilis

RecQ



[Bacillus subtilis]


2339
Hypothetical protein ypbB [Bacillus
UniRef100_P50728

Bacillus subtilis

YpbB




subtilis]



2340
Ferredoxin [Bacillus subtilis]
UniRef100_P50727

Bacillus subtilis



2341
Hypothetical protein ypaA [Bacillus
UniRef100_P50726

Bacillus subtilis

YpaA




subtilis]



2342


2343
D-3-phosphoglycerate dehydrogenase
UniRef100_P35136

Bacillus subtilis

SerA



[Bacillus subtilis]


2344
BH1600 protein [Bacillus halodurans]
UniRef100_Q9KCH1

Bacillus halodurans



2345
Sigma-X negative effector [Bacillus
UniRef100_P35166

Bacillus subtilis

RsiX




subtilis]



2346
RNA polymerase sigma factor sigX
UniRef100_P35165

Bacillus subtilis

SigX



[Bacillus subtilis]


2347
Transcriptional regulator [Bacillus
UniRef100_Q72XJ3

Bacillus cereus

LytR




cereus]



2348
Endo-1,4-beta-xylanase [Bacillus
UniRef100_Q9K630

Bacillus

YheN




halodurans]



halodurans



2349


2350
Alkaline phosphatase synthesis sensor
UniRef100_Q898N3

Clostridium tetani

YclK



protein phoR [Clostridium tetani]


2351
Response regulators consisting of a
UniRef100_Q8R9H7

Thermoanaerobacter

YycF



CheY-like receiver domain and a HTH


tengcongensis




DNA-binding domain



[Thermoanaerobacter tengcongensis]


2352
Sensor protein resE [Bacillus subtilis]
UniRef100_P35164

Bacillus subtilis

ResE


2353
Transcriptional regulatory protein resD
UniRef100_P35163

Bacillus subtilis

ResD



[Bacillus subtilis]


2354
Protein resC [Bacillus subtilis]
UniRef100_P35162

Bacillus subtilis

ResC


2355
ResB protein [Bacillus subtilis]
UniRef100_P35161

Bacillus subtilis

ResB


2356
Thiol-disulfide oxidoreductase resA
UniRef100_P35160

Bacillus subtilis

ResA



[Bacillus subtilis]


2357
Ribosomal large subunit pseudouridine
UniRef100_P35159

Bacillus subtilis

RluB



synthase B [Bacillus subtilis]


2358
Spore maturation protein B [Bacillus
UniRef100_P35158

Bacillus subtilis

SpmB




subtilis]



2359
Spore maturation protein A [Bacillus
UniRef100_P35157

Bacillus subtilis

SpmA




subtilis]



2360
Penicillin-binding protein 5* precursor
UniRef100_P35150

Bacillus subtilis

DacB



[Bacillus subtilis]


2361
Hypothetical protein ypul [Bacillus
UniRef100_P35156

Bacillus subtilis

YpuI




subtilis]



2362
Segregation and condensation protein
UniRef100_P35155

Bacillus subtilis

YpuH



B [Bacillus subtilis]


2363
Segregation and condensation protein
UniRef100_P35154

Bacillus subtilis

YpuG



A [Bacillus subtilis]


2364


2365
Hypothetical protein ypuF [Bacillus
UniRef100_P17617

Bacillus subtilis

YpuF




subtilis]



2366
RibT protein [Bacillus subtilis]
UniRef100_P17622

Bacillus subtilis

RibT


2367
6,7-dimethyl-8-ribityllumazine synthase
UniRef100_Q44681

Bacillus

RibH



[Bacillus amyloliquefaciens]


amyloliquefaciens



2368
Riboflavin biosynthesis protein ribA
UniRef100_P17620
Includes: GTP
RibA



[Includes: GTP cyclohydrolase II (EC

cyclohydrolase II



3.5.4.25); 3,4-dihydroxy-2-butanone 4-

(EC 3.5.4.25);



phosphate synthase (DHBP synthase)]

3,4-dihydroxy-2-



[Bacillus subtilis]

butanone 4-





phosphate





synthase (DHBP





synthase)


2369
Riboflavin synthase alpha chain
UniRef100_P16440

Bacillus subtilis

RibE



[Bacillus subtilis]


2370
Riboflavin biosynthesis protein ribD
UniRef100_P17618
Includes:
RibD



[Includes:

Diaminohydroxyphosphoribosylaminopyrimidine



Diaminohydroxyphosphoribosylamino-

deaminase (EC



pyrimidine deaminase (EC 3.5.4.26)

3.5.4.26)



(Riboflavin-specific deaminase); 5-

(Riboflavin-



amino-6-(5-phosphoribosylamino)uracil

specific



reductase (EC 1.1.1.193) (HTP

deaminase); 5-



reductase)] [Bacillus subtilis]

amino-6-(5-





phosphoribosylamino)uracil





reductase (EC





1.1.1.193) (HTP





reductase)


2371
Hypothetical protein ypuD [Bacillus
UniRef100_P17616

Bacillus subtilis

YpuD




subtilis]



2372
Putative serine/threonine protein
UniRef100_Q7VFC1

Helicobacter

YjbP



phosphatase [Helicobacter hepaticus]


hepaticus



2373
Stress response homolog Hsp [Bacillus
UniRef100_Q9X3Z5

Bacillus subtilis





subtilis]



2374
Response regulator aspartate
UniRef100_Q00828

Bacillus subtilis

RapA



phosphatase A [Bacillus subtilis]


2375
Peptidyl-prolyl cis-trans isomerase B
UniRef100_P35137

Bacillus subtilis

PpiB



[Bacillus subtilis]


2376



YpuA


2377
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012





anthracis str. A2012]



2378
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069


2379



YndL


2380
Diaminopimelate decarboxylase
UniRef100_P23630

Bacillus subtilis

LysA



[Bacillus subtilis]


2381


2382
Stage V sporulation protein AF [Bacillus
UniRef100_P31845

Bacillus subtilis

SpoVAF




subtilis]



2383
Stage V sporulation protein AE [Bacillus
UniRef100_P40870

Bacillus subtilis

SpoVAE




subtilis]



2384
Stage V sporulation protein AE [Bacillus
UniRef100_P40870

Bacillus subtilis

SpoVAE




subtilis]



2385
Stage V sporulation protein AD
UniRef100_P40869

Bacillus subtilis

SpoVAD



[Bacillus subtilis]


2386



SpoVAC


2387
Stage V sporulation protein AB [Bacillus
UniRef100_P40867

Bacillus subtilis

SpoVAB




subtilis]



2388
Stage V sporulation protein AA [Bacillus
UniRef100_P40866

Bacillus subtilis

SpoVAA




subtilis]



2389



SigF


2390



SpoIIAB


2391



SpoIIAA


2392
Penicillin-binding protein dacF
UniRef100_P38422

Bacillus subtilis

DacF



precursor [Bacillus subtilis]


2393
Purine nucleoside phosphorylase I
UniRef100_P46354

Bacillus subtilis

PunA



[Bacillus subtilis]


2394
Phosphopentomutase [Bacillus subtilis]
UniRef100_P46353

Bacillus subtilis

Drm


2395
Tyrosine recombinase xerD [Bacillus
UniRef100_P46352

Bacillus subtilis

RipX




subtilis]



2396


2397
Ferric uptake regulation protein
UniRef100_P54574

Bacillus subtilis

Fur



[Bacillus subtilis]


2398
Stage II sporulation protein M [Bacillus
UniRef100_P37873

Bacillus subtilis

SpoIIM




subtilis]



2399
Lmo2763 protein [Listeria
UniRef100_Q926Y4

Listeria

YdhO




monocytogenes]



monocytogenes



2400
UPI00003CA2F1 UniRef100 entry
UniRef100_UPI00003CA2F1


2401
Probable allantoin permease [Bacillus
UniRef100_P94575

Bacillus subtilis

YwoE




subtilis]



2402



YdaF


2403
Hypothetical protein yqkK [Bacillus
UniRef100_P54573

Bacillus subtilis





subtilis]



2404



YybD


2405
Hypothetical protein [Bacillus pumilus]
UniRef100_Q93PN4

Bacillus pumilus

YqxK


2406
ADP-ribose pyrophosphatase [Bacillus
UniRef100_P54570

Bacillus subtilis

NudF




subtilis]



2407


2408
YdgC protein [Bacillus subtilis]
UniRef100_P96701

Bacillus subtilis

YdgC


2409
Hypothetical protein ydgD [Bacillus
UniRef100_P96702

Bacillus subtilis

YdgD




subtilis]



2410
Hypothetical oxidoreductase yqkF
UniRef100_P54569

Bacillus subtilis

YqkF



[Bacillus subtilis]


2411


2412



YqkD


2413
Hypothetical protein yqkC [Bacillus
UniRef100_P54566

Bacillus subtilis





subtilis]



2414
Hypothetical protein yqkB [Bacillus
UniRef100_P54565

Bacillus subtilis

YqkB




subtilis]



2415
UPI00003CBE2B UniRef100 entry
UniRef100_UPI00003CBE2B


2416
Hypothetical UPF0157 protein yqkA
UniRef100_P54564

Bacillus subtilis

YqkA



[Bacillus subtilis]


2417
Hypothetical protein yqjZ [Bacillus
UniRef100_P54563

Bacillus subtilis

YqjZ




subtilis]



2418
Lipase precursor [Bacillus subtilis]
UniRef100_P37957

Bacillus subtilis

Lip


2419


2420
Nickel ABC transporter [Bacillus
UniRef100_Q9KBX8

Bacillus

AppA




halodurans]



halodurans



2421
Nickel ABC transporter [Bacillus
UniRef100_Q9KBX7

Bacillus

AppB




halodurans]



halodurans



2422
Nickel ABC transporter [Bacillus
UniRef100_Q9KBX6

Bacillus

AppC




halodurans]



halodurans



2423
Putative oligopeptide ABC transporter
UniRef100_Q895A4

Clostridium tetani

DppD



[Clostridium tetani]


2424
Putative oligopeptide ABC transporter
UniRef100_Q895A5

Clostridium tetani

OppF



[Clostridium tetani]


2425
DNA-damage-inducible protein [Bacillus
UniRef100_Q9KDR1

Bacillus

YojI




halodurans]



halodurans



2426
Fibronectin-binding protein [Bacillus
UniRef100_Q63CW1

Bacillus cereus ZK





cereus ZK]



2427
Hypothetical protein yolD
UniRef100_O64030
Bacteriophage
YolD



[Bacteriophage SPBc2]


SPBc2



2428



UvrX


2429
Hypothetical protein yqzH [Bacillus
UniRef100_O32014

Bacillus subtilis





subtilis]



2430
Hypothetical transport protein yqjV
UniRef100_P54559

Bacillus subtilis

YqjV



[Bacillus subtilis]


2431
Hypothetical protein yqjT [Bacillus
UniRef100_P54557

Bacillus subtilis

YqjT




subtilis]



2432



CoaA


2433
LacG [Lactococcus lactis]
UniRef100_Q9RAU9

Lactococcus lactis



2434
LacF [Lactococcus lactis]
UniRef100_Q9RAV2

Lactococcus

YdbJ






lactis



2435


2436
Hypothetical protein [Bacillus
UniRef100_Q70K07

Bacillus amyloliquefaciens





amyloliquefaciens]



2437
Hypothetical protein [Bacillus cereus]
UniRef100_Q737G3

Bacillus cereus



2438
YmaC protein [Bacillus subtilis]
UniRef100_O31789

Bacillus subtilis

YmaC


2439
Hypothetical oxidoreductase yqjQ
UniRef100_P54554

Bacillus subtilis

YqjQ



[Bacillus subtilis]


2440



YqjP


2441



ProI


2442
Hypothetical protein yqjN [Bacillus
UniRef100_P54551

Bacillus subtilis

YqjN




subtilis]



2443
Probable NADH-dependent flavin
UniRef100_P54550

Bacillus subtilis

YqjM



oxidoreductase yqjM [Bacillus subtilis]


2444
Hypothetical protein yqjL [Bacillus
UniRef100_P54549

Bacillus subtilis

YqjL




subtilis]



2445


2446
Ribonuclease Z [Bacillus subtilis]
UniRef100_P54548

Bacillus subtilis

YqjK


2447
Glucose-6-phosphate 1-dehydrogenase
UniRef100_P54547

Bacillus subtilis

Zwf



[Bacillus subtilis]


2448
Hypothetical conserved protein
UniRef100_Q8ELZ9

Oceanobacillus

CitH



[Oceanobacillus iheyensis]


iheyensis



2449
Hypothetical protein OB3065
UniRef100_Q8ELZ8

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


2450
Immunogenic protein [Oceanobacillus
UniRef100_Q8ELZ7

Oceanobacillus

SsuA




iheyensis]



iheyensis



2451
YdhQ protein [Bacillus subtilis]
UniRef100_O05509

Bacillus subtilis

YdhQ


2452
Beta-glucosidase [Bacillus halodurans]
UniRef100_Q9K615

Bacillus

YdhP






halodurans



2453
Putative cellobiose-specific enzyme IIC
UniRef100_Q8KP28

Bacillus pumilus

YdhO



[Bacillus pumilus]


2454
YdhO protein [Bacillus subtilis]
UniRef100_O05507

Bacillus subtilis

YdhO


2455
YdhN protein [Bacillus subtilis]
UniRef100_O05506

Bacillus subtilis

YdhN


2456
PTS system, cellobiose-specific
UniRef100_Q9K613

Bacillus halodurans




enzyme II, B component [Bacillus




halodurans]



2457
Alkaline phosphatase [Bacillus cereus
UniRef100_Q639W1

Bacillus cereus

PhoB



ZK]

ZK


2458
Glucose 1-dehydrogenase A [Bacillus
UniRef100_P10528

Bacillus

Gdh




megaterium]



megaterium



2459
6-phosphogluconate dehydrogenase,
UniRef100_P80859

Bacillus subtilis

YqjI



decarboxylating II [Bacillus subtilis]


2460
DNA polymerase IV 1 [Bacillus subtilis]
UniRef100_P54545

Bacillus subtilis

YqjH


2461
Hypothetical protein yqzJ [Bacillus
UniRef100_Q7WY64

Bacillus subtilis





subtilis]



2462



YqjG


2463
Hypothetical protein yqjE [Bacillus
UniRef100_P54542

Bacillus subtilis

YqjE




subtilis]



2464
Methylmalonyl-CoA decarboxylase
UniRef100_Q9K8P6

Bacillus

YqjD



alpha subunit [Bacillus halodurans]


halodurans



2465
Hypothetical protein yqjA [Bacillus
UniRef100_P54538

Bacillus subtilis

YqjA




subtilis]



2466
Probable amino-acid ABC transporter
UniRef100_P54537

Bacillus subtilis

YqiZ



ATP-binding protein yqiZ [Bacillus




subtilis]



2467
Probable amino-acid ABC transporter
UniRef100_P54536

Bacillus subtilis

YqiY



permease protein yqiY [Bacillus subtilis]


2468
Probable amino-acid ABC transporter
UniRef100_P54535

Bacillus subtilis

YqiX



extracellular binding protein yqiX



precursor [Bacillus subtilis]


2469
Hypothetical protein yqiW [Bacillus
UniRef100_P54534

Bacillus subtilis

YqiW




subtilis]



2470
Protein bmrU [Bacillus subtilis]
UniRef100_P39074

Bacillus subtilis

BmrU


2471
Lipoamide acyltransferase component
UniRef100_P37942

Bacillus subtilis

BkdB



of branched-chain alpha-keto acid



dehydrogenase complex (EC 2.3.1.168)



(Dihydrolipoyllysine-residue (2-



methylpropanoyl)transferase) [Bacillus




subtilis]



2472
2-oxoisovalerate dehydrogenase beta
UniRef100_P37941

Bacillus subtilis

BkdAB



subunit [Bacillus subtilis]


2473
2-oxoisovalerate dehydrogenase alpha
UniRef100_P37940

Bacillus subtilis

BkdAA



subunit [Bacillus subtilis]


2474
Dihydrolipoyl dehydrogenase [Bacillus
UniRef100_P54533

Bacillus subtilis

LpdV




subtilis]



2475
Probable butyrate kinase [Bacillus
UniRef100_P54532

Bacillus subtilis

Buk




subtilis]



2476
Leucine dehydrogenase [Bacillus
UniRef100_P54531

Bacillus subtilis

Bcd




subtilis]



2477
Probable phosphate butyryltransferase
UniRef100_P54530

Bacillus subtilis

Ptb



[Bacillus subtilis]


2478
Putative sigma L-dependent
UniRef100_P54529

Bacillus subtilis

BkdR



transcriptional regulator yqiR [Bacillus




subtilis]



2479
Hypothetical protein yqzF [Bacillus
UniRef100_O32015

Bacillus subtilis





subtilis]



2480
Hypothetical protein yqiK [Bacillus
UniRef100_P54527

Bacillus subtilis

YqiK




subtilis]



2481
Hypothetical protein yqiI [Bacillus
UniRef100_P54525

Bacillus subtilis

YqiI




subtilis]



2482



YqiH


2483
Serine O-acetyltransferase
UniRef100_Q8PSY4

Methanosarcina

CysE



[Methanosarcina mazei]


mazei



2484


2485
Stage 0 sporulation protein A [Bacillus
UniRef100_P06534

Bacillus subtilis

Spo0A




subtilis]



2486



SpoIVB


2487
DNA repair protein recN [Bacillus
UniRef100_Q659H4

Bacillus

RecN




amyloliquefaciens]



amyloliquefaciens



2488
Arginine repressor [Bacillus subtilis]
UniRef100_P17893

Bacillus subtilis

AhrC


2489
Hypothetical protein yqxC [Bacillus
UniRef100_P19672

Bacillus subtilis

YqxC




subtilis]



2490



Dxs


2491
Geranyltranstransferase [Bacillus
UniRef100_P54383

Bacillus subtilis

YqiD




subtilis]



2492
Probable exodeoxyribonuclease VII
UniRef100_P54522

Bacillus subtilis




small subunit [Bacillus subtilis]


2493
Probable exodeoxyribonuclease VII
UniRef100_P54521

Bacillus subtilis

YqiB



large subunit [Bacillus subtilis]


2494
FolD bifunctional protein [Includes:
UniRef100_P54382
Includes:
FolD



Methylenetetrahydrofolate

Methylenetetrahydrofolate



dehydrogenase (EC 1.5.1.5);

dehydrogenase



Methenyltetrahydrofolate

(EC 1.5.1.5);



cyclohydrolase (EC 3.5.4.9)] [Bacillus

Methenyltetrahydrofolate




subtilis]


cyclohydrolase





(EC 3.5.4.9)


2495
N utilization substance protein B
UniRef100_P54520

Bacillus subtilis

NusB



homolog [Bacillus subtilis]


2496
Hypothetical protein yqhY [Bacillus
UniRef100_P54519

Bacillus subtilis

YqhY




subtilis]



2497



AccC


2498
Biotin carboxyl carrier protein of acetyl-
UniRef100_P49786

Bacillus subtilis

AccB



CoA carboxylase [Bacillus subtilis]


2499
Stage III sporulation protein AH
UniRef100_P49785

Bacillus subtilis

SpoIIIAH



[Bacillus subtilis]


2500
Stage III sporulation protein AG
UniRef100_P49784

Bacillus subtilis

SpoIIIAG



[Bacillus subtilis]


2501
Stage III sporulation protein AF
UniRef100_P49783

Bacillus subtilis

SpoIIIAF



[Bacillus subtilis]


2502
Stage III sporulation protein AE
UniRef100_P49782

Bacillus subtilis

SpoIIIAE



[Bacillus subtilis]


2503
Stage III sporulation protein AD
UniRef100_P49781

Bacillus subtilis

SpoIIIAD



[Bacillus subtilis]


2504
Stage III sporulation protein AC
UniRef100_P49780

Bacillus subtilis




[Bacillus subtilis]


2505
Stage III sporulation protein AB
UniRef100_Q01368

Bacillus subtilis

SpoIIIAB



[Bacillus subtilis]


2506



SpoIIIAA


2507
Hypothetical protein yqhV [Bacillus
UniRef100_P49779

Bacillus subtilis





subtilis]



2508
Elongation factor P [Bacillus subtilis]
UniRef100_P49778

Bacillus subtilis

Efp


2509
Putative peptidase yqhT [Bacillus
UniRef100_P54518

Bacillus subtilis

YqhT




subtilis]



2510
3-dehydroquinate dehydratase [Bacillus
UniRef100_P54517

Bacillus subtilis

YqhS




subtilis]



2511
Hypothetical protein yqhR [Bacillus
UniRef100_P54516

Bacillus subtilis

YqhR




subtilis]



2512
Hypothetical protein yqhQ [Bacillus
UniRef100_P54515

Bacillus subtilis

YqhQ




subtilis]



2513
Hypothetical protein yqhP [Bacillus
UniRef100_P54514

Bacillus subtilis

YqhP




subtilis]



2514
Hypothetical protein yqhO [Bacillus
UniRef100_P54513

Bacillus subtilis

YqhO




subtilis]



2515
Transcriptional regulator mntR [Bacillus
UniRef100_P54512

Bacillus subtilis

MntR




subtilis]



2516
Hypothetical protein yqhM [Bacillus
UniRef100_P54511

Bacillus subtilis

YqhM




subtilis]



2517
Hypothetical protein yqhL [Bacillus
UniRef100_P54510

Bacillus subtilis

YqhL




subtilis]



2518
Glycine betaine-binding protein
UniRef100_P46922

Bacillus subtilis

OpuAC



precursor [Bacillus subtilis]


2519
Glycine betaine transport system
UniRef100_P46921

Bacillus subtilis

OpuAB



permease protein opuAB [Bacillus




subtilis]



2520
Glycine betaine transport ATP-binding
UniRef100_P46920

Bacillus subtilis

OpuAA



protein opuAA [Bacillus subtilis]


2521
Probable glycine dehydrogenase
UniRef100_P54377
decarboxylating
GcvPB



[decarboxylating] subunit 2 [Bacillus




subtilis]



2522
Probable glycine dehydrogenase
UniRef100_P54376
decarboxylating
GcvPA



[decarboxylating] subunit 1 [Bacillus




subtilis]





subtilis]



2523
Aminomethyltransferase [Bacillus
UniRef100_P54378

Bacillus subtilis

GcvT




subtilis]



2524
Hypothetical helicase yqhH [Bacillus
UniRef100_P54509

Bacillus subtilis

YqhH




subtilis]



2525
Hypothetical protein yqhG [Bacillus
UniRef100_P54508

Bacillus subtilis

YqhG




subtilis]



2526


2527



SinR


2528
Spore coat-associated protein N
UniRef100_P54507

Bacillus subtilis

TasA



[Bacillus subtilis]


2529
Signal peptidase I W [Bacillus subtilis]
UniRef100_P54506

Bacillus subtilis

SipW


2530
Hypothetical protein yqxM [Bacillus
UniRef100_P40949

Bacillus subtilis

YqxM




subtilis]



2531
YqzG protein [Bacillus subtilis]
UniRef100_O32019

Bacillus subtilis

YqzG


2532
YqzE protein [Bacillus subtilis]
UniRef100_O32020

Bacillus subtilis



2533



ComGG


2534



ComGF


2535
ComG operon protein 5 precursor
UniRef100_P25957

Bacillus subtilis

ComGE



[Bacillus subtilis]


2536
ComG operon protein 4 precursor
UniRef100_P25956

Bacillus subtilis

ComGD



[Bacillus subtilis]


2537
ComG operon protein 3 precursor
UniRef100_P25955

Bacillus subtilis




[Bacillus subtilis]


2538



ComGB


2539
ComG operon protein 1 [Bacillus
UniRef100_P25953

Bacillus subtilis

ComGA




subtilis]



2540
Hypothetical protein yqhA [Bacillus
UniRef100_P54504

Bacillus subtilis

YqhA




subtilis]



2541
Hypothetical protein yqgZ [Bacillus
UniRef100_P54503

Bacillus subtilis

YqgZ




subtilis]



2542
Hypothetical protein yqgY [Bacillus
UniRef100_P54502

Bacillus subtilis





subtilis]



2543
Hypothetical protein yqgX [Bacillus
UniRef100_P54501

Bacillus subtilis

YqgX




subtilis]



2544
Hypothetical protein yqgW [Bacillus
UniRef100_P54500

Bacillus subtilis





subtilis]



2545
Hypothetical protein [Staphylococcus
UniRef100_Q8CSE8

Staphylococcus epidermidis





epidermidis]



2546



YqgU


2547
Hypothetical protein yqgT [Bacillus
UniRef100_P54497

Bacillus subtilis

YqgT




subtilis]



2548
Ferrichrome-binding protein precursor
UniRef100_P37580

Bacillus subtilis

FhuD



[Bacillus subtilis]


2549
Hypothetical protein yqgS [Bacillus
UniRef100_P54496

Bacillus subtilis

YqgS




subtilis]



2550
Glucokinase [Bacillus subtilis]
UniRef100_P54495

Bacillus subtilis

GlcK


2551
Hypothetical protein yqgQ [Bacillus
UniRef100_P54494

Bacillus subtilis





subtilis]



2552
Stage V sporulation protein AF
UniRef100_Q8EQ08

Oceanobacillus

SpoVAF



[Oceanobacillus iheyensis]


iheyensis



2553
Hypothetical protein yqgP [Bacillus
UniRef100_P54493

Bacillus subtilis

YqgP




subtilis]



2554
Hypothetical protein yqgO [Bacillus
UniRef100_P54492

Bacillus subtilis





subtilis]



2555
Hypothetical protein yqgN [Bacillus
UniRef100_P54491

Bacillus subtilis

YqgN




subtilis]



2556


2557



YqgM


2558
Hypothetical protein yqgL [Bacillus
UniRef100_P54489

Bacillus subtilis

YqgL




subtilis]



2559



YqzD


2560
YqzC protein [Bacillus subtilis]
UniRef100_O32023

Bacillus subtilis

YqzC


2561
Phosphate import ATP-binding protein
UniRef100_P46342

Bacillus subtilis

PstBB



pstB 1 [Bacillus subtilis]


2562
Phosphate import ATP-binding protein
UniRef100_P46341

Bacillus subtilis

PstBA



pstB 2 [Bacillus subtilis]


2563
Probable ABC transporter permease
UniRef100_P46340

Bacillus subtilis

PstA



protein yqqI [Bacillus subtilis]


2564
Probable ABC transporter permease
UniRef100_P46339

Bacillus subtilis

PstC



protein yqgH [Bacillus subtilis]


2565
Probable ABC transporter binding
UniRef100_P46338

Bacillus subtilis

PstS



protein yqgG precursor [Bacillus




subtilis]



2566
Hypothetical protein yqgF [Bacillus
UniRef100_P54488

Bacillus subtilis

PbpA




subtilis]



2567
Hypothetical protein yqgE [Bacillus
UniRef100_P54487

Bacillus subtilis

YqgE




subtilis]



2568



SodA


2569
Hypothetical protein yqgC [Bacillus
UniRef100_P54486

Bacillus subtilis

YqgC




subtilis]



2570
Hypothetical protein yqgB [Bacillus
UniRef100_P54485

Bacillus subtilis

YqgB




subtilis]



2571
Hypothetical protein yqfZ [Bacillus
UniRef100_P54483

Bacillus subtilis





subtilis]



2572
4-hydroxy-3-methylbut-2-en-1-yl
UniRef100_P54482

Bacillus subtilis

YqfY



diphosphate synthase (EC 1.17.4.3) (1-



hydroxy-2-methyl-2-(E)-butenyl 4-



diphosphate synthase) [Bacillus subtilis]


2573
Hypothetical protein yqfX [Bacillus
UniRef100_P54481

Bacillus subtilis

YqfX




subtilis]



2574
Putative nucleotidase yqfW [Bacillus
UniRef100_P54480

Bacillus subtilis

YqfW




subtilis]



2575
Zinc-specific metalloregulatory protein
UniRef100_P54479

Bacillus subtilis

Zur



[Bacillus subtilis]


2576
Metal (Zinc) transport protein [Listeria
UniRef100_Q926D9

Listeria innocua

YceA




innocua]



2577



YcdI


2578
Hypothetical protein yqfU [Bacillus
UniRef100_P54478

Bacillus subtilis

YqfU




subtilis]



2579


2580
Probable endonuclease IV [Bacillus
UniRef100_P54476

Bacillus subtilis

YqfS




subtilis]



2581
Probable RNA helicase yqfR [Bacillus
UniRef100_P54475

Bacillus subtilis

YqfR




subtilis]



2582
Hypothetical protein yqfQ [Bacillus
UniRef100_P54474

Bacillus subtilis

YqfQ




subtilis]



2583
4-hydroxy-3-methylbut-2-enyl
UniRef100_P54473

Bacillus subtilis

YqfP



diphosphate reductase [Bacillus




subtilis]



2584



YqfO


2585
Hypothetical protein yqfN [Bacillus
UniRef100_P54471

Bacillus subtilis

YqfN




subtilis]



2586
YwqL protein [Bacillus subtilis]
UniRef100_P96724

Bacillus subtilis

YwqL


2587


2588


2589


2590


2591
Hypothetical protein CAC0336
UniRef100_Q97M62

Clostridium acetobutylicum




[Clostridium acetobutylicum]


2592
Hypothetical protein [Bacillus
UniRef100_Q6HGF2

Bacillus thuringiensis





thuringiensis]



2593


2594
YwqJ protein [Bacillus subtilis]
UniRef100_P96722

Bacillus subtilis

YwqJ


2595
Hypothetical protein ywqI [Bacillus
UniRef100_P96721

Bacillus subtilis





subtilis]



2596



YwqH


2597
Cytochrome c-550 [Bacillus subtilis]
UniRef100_P24469

Bacillus subtilis

CccA


2598



SigA


2599
DNA primase [Bacillus subtilis]
UniRef100_P05096

Bacillus subtilis

DnaG


2600
Hypothetical UPF0178 protein yqxD
UniRef100_P17868

Bacillus subtilis

YqxD



[Bacillus subtilis]


2601
Hypothetical UPF0085 protein yqfL
UniRef100_P54470

Bacillus subtilis

YqfL



[Bacillus subtilis]


2602
YqzB protein [Bacillus subtilis]
UniRef100_O34994

Bacillus subtilis

YqzB


2603
Glycyl-tRNA synthetase beta chain
UniRef100_P54381

Bacillus subtilis

GlyS



[Bacillus subtilis]


2604
Glycyl-tRNA synthetase alpha chain
UniRef100_P54380

Bacillus subtilis

GlyQ



[Bacillus subtilis]


2605
DNA repair protein recO [Bacillus
UniRef100_P42095

Bacillus subtilis

RecO




subtilis]



2606


2607
GTP-binding protein era homolog
UniRef100_P42182

Bacillus subtilis

Era



[Bacillus subtilis]


2608
Cytidine deaminase [Bacillus subtilis]
UniRef100_P19079

Bacillus subtilis

Cdd


2609


2610
Hypothetical UPF0054 protein yqfG
UniRef100_P46347

Bacillus subtilis

YqfG



[Bacillus subtilis]


2611
Hypothetical protein yqfF [Bacillus
UniRef100_P46344

Bacillus subtilis

YqfF




subtilis]



2612
PhoH-like protein [Bacillus subtilis]
UniRef100_P46343

Bacillus subtilis

PhoH


2613



YqfD


2614
Hypothetical protein yqfC [Bacillus
UniRef100_P54468

Bacillus subtilis





subtilis]



2615
Hypothetical protein yqfB [Bacillus
UniRef100_P54467

Bacillus subtilis

YqfB




subtilis]



2616
Hypothetical protein yqfA [Bacillus
UniRef100_P54466

Bacillus subtilis

YqfA




subtilis]



2617



YqeZ


2618
Hypothetical protein yqeY [Bacillus
UniRef100_P54464

Bacillus subtilis

YqeY




subtilis]



2619


2620
Hypothetical protein yqeW [Bacillus
UniRef100_P54463

Bacillus subtilis

YqeW




subtilis]



2621
Deoxyribose-phosphate aldolase
UniRef100_Q92A19

Listeria innocua

Dra



[Listeria innocua]


2622
Hypothetical UPF0004 protein yqeV
UniRef100_P54462

Bacillus subtilis

YqeV



[Bacillus subtilis]


2623
Hypothetical UPF0088 protein yqeU
UniRef100_P54461

Bacillus subtilis

YqeU



[Bacillus subtilis]


2624
Ribosomal protein L11
UniRef100_P54460

Bacillus subtilis

YqeT



methyltransferase [Bacillus subtilis]


2625
Chaperone protein dnaJ [Bacillus
UniRef100_P17631

Bacillus subtilis

DnaJ




subtilis]



2626



DnaK


2627


2628


2629
Heat-inducible transcription repressor
UniRef100_P25499

Bacillus subtilis

HrcA



hrcA [Bacillus subtilis]


2630
Probable oxygen-independent
UniRef100_P54304

Bacillus subtilis

HemN



coproporphyrinogen III oxidase



[Bacillus subtilis]


2631



LepA


2632
Hypothetical protein yqxA [Bacillus
UniRef100_P38425

Bacillus subtilis

YqxA




subtilis]



2633



SpoIIP


2634
Germination protease precursor
UniRef100_P22322

Bacillus subtilis

Gpr



[Bacillus subtilis]


2635
30S ribosomal protein S20 [Bacillus
UniRef100_P21477

Bacillus subtilis





subtilis]



2636
Hypothetical protein yqeN [Bacillus
UniRef100_P54459

Bacillus subtilis

YqeN




subtilis]



2637


2638
ComE operon protein 3 [Bacillus
UniRef100_P39695

Bacillus subtilis

ComEC




subtilis]



2639
ComE operon protein 2 [Bacillus
UniRef100_P32393

Bacillus subtilis

ComEB




subtilis]



2640
ComE operon protein 1 [Bacillus
UniRef100_P39694

Bacillus subtilis

ComEA




subtilis]



2641
ComE operon protein 4 [Bacillus
UniRef100_P39696

Bacillus subtilis

ComER




subtilis]



2642
Hypothetical protein yqeM [Bacillus
UniRef100_P54458

Bacillus subtilis

YqeM




subtilis]



2643
Hypothetical protein yqeL [Bacillus
UniRef100_P54457

Bacillus subtilis

YqeL




subtilis]



2644
Hypothetical protein yqeK [Bacillus
UniRef100_P54456

Bacillus subtilis

YqeK




subtilis]



2645
Nicotinate-nucleotide
UniRef100_P54455

Bacillus subtilis

YqeJ



adenylyltransferase (EC 2.7.7.18)



(Deamido-NAD(+) pyrophosphorylase)



(Deamido-NAD(+) diphosphorylase)



[Bacillus subtilis]


2646
Hypothetical UPF0044 protein yqeI
UniRef100_P54454

Bacillus subtilis




[Bacillus subtilis]


2647
Shikimate dehydrogenase [Bacillus
UniRef100_P54374

Bacillus subtilis

AroD




subtilis]



2648
Hypothetical protein yqeH [Bacillus
UniRef100_P54453

Bacillus subtilis

YqeH




subtilis]



2649
Hypothetical protein yqeG [Bacillus
UniRef100_P54452

Bacillus subtilis

YqeG




subtilis]



2650


2651


2652
Hypothetical lipoprotein yqeF precursor
UniRef100_P54451

Bacillus subtilis

YqeF



[Bacillus subtilis]


2653
Acetyltransferase, GNAT family
UniRef100_Q81KW8

Bacillus

YdfB



[Bacillus anthracis]


anthracis



2654
Hypothetical protein yrhF [Bacillus
UniRef100_O05398

Bacillus subtilis

YrhF




subtilis]



2655
Formate dehydrogenase chain A
UniRef100_O05397

Bacillus subtilis

YrhE



[Bacillus subtilis]


2656
Hypothetical protein yrhD [Bacillus
UniRef100_O05396

Bacillus subtilis

YrhD




subtilis]



2657


2658
RNA polymerase sigma-K factor
UniRef100_P12254

Bacillus subtilis

SpoIIIC



precursor [Bacillus subtilis]


2659



YcnB


2660
BH2157 protein [Bacillus halodurans]
UniRef100_Q9KAX9

Bacillus

YuaI






halodurans



2661


2662


2663
Alanyl-tRNA synthetase family protein
UniRef100_Q81Y73

Bacillus

AlaS



[Bacillus anthracis]


anthracis



2664
METAL-ACTIVATED PYRIDOXAL
UniRef100_Q8YCI2

Brucella melitensis




ENZYME [Brucella melitensis]


2665
Probable translation initiation inhibitor
UniRef100_Q6LKM3

Photobacterium

YabJ



[Photobacterium profundum)]


profundum)



2666



YccC


2667
Putative threonine synthase
UniRef100_Q82IF6

Streptomyces

ThrC



[Streptomyces avermitilis]


avermitilis



2668



YabJ


2669
Hypothetical protein
UniRef100_Q8RBA0

Thermoanaerobacter




[Thermoanaerobacter tengcongensis]


tengcongensis



2670


2671
UPI000032CE59 UniRef100 entry
UniRef100_UPI000032CE59


2672
Multidrug-efflux transporter 1 regulator
UniRef100_P39075

Bacillus subtilis

BmrR



[Bacillus subtilis]


2673
Metallo-beta-lactamase/rhodanese-like
UniRef100_Q81Q95

Bacillus

YrkH



domain protein [Bacillus anthracis]


anthracis



2674
Hypothetical protein [Bacillus cereus
UniRef100_Q63B51

Bacillus cereus

YumB



ZK]

ZK


2675
NreC [Staphylococcus carnosus]
UniRef100_Q7WZY4

Staphylococcus

DegU






carnosus



2676
Two-component sensor histidine kinase
UniRef100_Q67JE7

Symbiobacterium

DegS



[Symbiobacterium thermophilum]


thermophilum



2677
YdfQ protein [Bacillus subtilis]
UniRef100_P96695

Bacillus subtilis

YdfQ


2678
Hypothetical Membrane Spanning
UniRef100_Q813Y5

Bacillus cereus

YrkJ



Protein [Bacillus cereus]


2679
Hypothetical UPF0033 protein yrkI
UniRef100_P54436

Bacillus subtilis




[Bacillus subtilis]


2680
UPI00003CB3C6 UniRef100 entry
UniRef100_UPI00003CB3C6

YrkH


2681
Molybdopterin biosynthesis MoeB
UniRef100_Q81HL2

Bacillus cereus

YrkF



protein [Bacillus cereus]


2682
Hypothetical protein yrkE [Bacillus
UniRef100_P54432

Bacillus subtilis

YrkE




subtilis]



2683
Hypothetical conserved protein
UniRef100_Q8EN37

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


2684
S-adenosylmethionine-dependent
UniRef100_Q97FB3

Clostridium

YcgJ



methyltransferase [Clostridium


acetobutylicum





acetobutylicum]



2685
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81N81

Bacillus anthracis



2686
YciB protein [Bacillus subtilis]
UniRef100_P94399

Bacillus subtilis



2687
Acetylxylan esterase related enzyme
UniRef100_Q97LM8

Clostridium acetobutylicum




[Clostridium acetobutylicum]


2688
Hypothetical UPF0161 protein
UniRef100_P61464

Bacillus cereus




BCE4947 [Bacillus cereus]


2689
Delta-aminolevulinic acid dehydratase
UniRef100_Q9K8G2

Bacillus halodurans




[Bacillus halodurans]


2690
6-phospho-3-hexuloisomerase [Bacillus
UniRef100_Q6TV53

Bacillus

HxlB




methanolicus]



methanolicus



2691
Probable hexulose-6-phosphate
UniRef100_P42405

Bacillus subtilis

HxlA



synthase [Bacillus subtilis]


2692
Transcriptional regulator [Bacillus
UniRef100_Q70KJ9

Bacillus

HxlR




amyloliquefaciens]



amyloliquefaciens



2693
Fatty acid desaturase [Bacillus subtilis]
UniRef100_O34653

Bacillus subtilis

Des


2694
Sensor kinase [Bacillus subtilis]
UniRef100_O34757

Bacillus subtilis

YocF


2695
Sensor regulator [Bacillus subtilis]
UniRef100_O34723

Bacillus subtilis

YocG


2696
UPI00003CC1E4 UniRef100 entry
UniRef100_UPI00003CC1E4

YcgT


2697
Nickel transport system [Bacillus
UniRef100_Q9KFB8

Bacillus

AppA




halodurans]



halodurans



2698
Nickel transport system [Bacillus
UniRef100_Q9KFB7

Bacillus

AppB




halodurans]



halodurans



2699
Nickel transport system [Bacillus
UniRef100_Q9KFB6

Bacillus

AppC




halodurans]



halodurans



2700
Oligopeptide ABC transporter [Bacillus
UniRef100_Q9KFB5

Bacillus

DppD




halodurans]



halodurans



2701
Oligopeptide ABC transporter [Bacillus
UniRef100_Q9KFB4

Bacillus

AppF




halodurans]



halodurans



2702
UPI00003CB880 UniRef100 entry
UniRef100_UPI00003CB880

YdfL


2703
UPI00003CA374 UniRef100 entry
UniRef100_UPI00003CA374

YoeA


2704
PROBABLE TRANSCRIPTION
UniRef100_Q8XS91

Ralstonia solanacearum




REGULATOR PROTEIN [Ralstonia




solanacearum]



2705
Short chain dehydrogenase family
UniRef100_Q834I5

Enterococcus

YvaG



protein [Enterococcus faecalis]


faecalis



2706
Uncharacterized protein, containing
UniRef100_Q97L24

Clostridium acetobutylicum




predicted phosphatase domain



[Clostridium acetobutylicum]


2707
UPI000025758C UniRef100 entry
UniRef100_UPI000025758C

PnbA


2708
Cytochrome P450 [Bacillus subtilis]
UniRef100_O08469

Bacillus subtilis

CypA


2709
YtnM [Bacillus subtilis]
UniRef100_O34430

Bacillus subtilis

YtnM


2710
Hypothetical protein yndA precursor
UniRef100_O31805

Bacillus subtilis

YndA



[Bacillus subtilis]


2711
YvaG protein [Bacillus subtilis]
UniRef100_O32229

Bacillus subtilis

YvaG


2712
Levanase precursor [Bacillus subtilis]
UniRef100_P05656

Bacillus subtilis

SacC


2713
PTS system, fructose-specific IID
UniRef100_P26382

Bacillus subtilis

LevG



component [Bacillus subtilis]


2714
PTS system, fructose-specific IIC
UniRef100_P26381

Bacillus subtilis

LevF



component [Bacillus subtilis]


2715
PTS system, fructose-specific IIB
UniRef100_P26380

Bacillus subtilis

LevE



component [Bacillus subtilis]


2716
PTS system, fructose-specific IIA
UniRef100_P26379

Bacillus subtilis

LevD



component [Bacillus subtilis]


2717
Transcriptional regulatory protein levR
UniRef100_P23914

Bacillus subtilis

LevR



[Bacillus subtilis]


2718
Hypothetical protein [Bacillus cereus]
UniRef100_Q734Q5

Bacillus cereus



2719
Hypothetical protein yrhK [Bacillus
UniRef100_O05401

Bacillus subtilis





subtilis]



2720
UPI00003CB785 UniRef100 entry
UniRef100_UPI00003CB785


2721



AdaA


2722
Methylated-DNA--protein-cysteine S-
UniRef100_Q732Y7

Bacillus cereus

AdaB



methyltransferase [Bacillus cereus]


2723
Oxidoreductase, aldo/keto reductase
UniRef100_Q6HBJ5

Bacillus

YtbE



family [Bacillus thuringiensis]


thuringiensis



2724



YtbD


2725
Hypothetical UPF0087 protein ytcD
UniRef100_O34533

Bacillus subtilis

YtcD



[Bacillus subtilis]


2726


2727
Hypothetical protein yjjA [Bacillus
UniRef100_O34394

Bacillus subtilis

YjjA




subtilis]



2728
UPI000028298B UniRef100 entry
UniRef100_UPI000028298B


2729



YxeB


2730
Putative HTH-type transcriptional
UniRef100_P37499

Bacillus subtilis

YybE



regulator yybE [Bacillus subtilis]


2731
Hypothetical transport protein yybF
UniRef100_P37498

Bacillus subtilis

YybF



[Bacillus subtilis]


2732
Hypothetical protein [Bacillus cereus]
UniRef100_Q732J0

Bacillus cereus



2733
Probable bifunctional P-450: NADPH-
UniRef100_O08336
Includes:
YrhJ



P450 reductase 2 [Includes:

Cytochrome



Cytochrome P450 102 (EC 1.14.14.1);

P450 102 (EC



NADPH--cytochrome P450 reductase

1.14.14.1);



(EC 1.6.2.4)] [Bacillus subtilis]

NADPH--





cytochrome





P450 reductase





(EC 1.6.2.4)


2734
Regulatory protein [Bacillus subtilis]
UniRef100_O08335

Bacillus subtilis

YrhI


2735



WprA


2736
YrhH [Bacillus subtilis]
UniRef100_O05400

Bacillus subtilis

YrhH


2737


2738


2739


2740
Cystathionine gamma-lyase [Bacillus
UniRef100_O05394

Bacillus subtilis

YrhB




subtilis]



2741
Cysteine synthase [Bacillus subtilis]
UniRef100_O05393

Bacillus subtilis

YrhA


2742
MTA/SAH nucleosidase [Bacillus
UniRef100_O32028

Bacillus subtilis

Mtn




subtilis]



2743
YrrT protein [Bacillus subtilis]
UniRef100_O32029

Bacillus subtilis

YrrT


2744
Hypothetical protein yrzA [Bacillus
UniRef100_O32030

Bacillus subtilis





subtilis]



2745



YrrS


2746
YrrR protein [Bacillus subtilis]
UniRef100_O32032

Bacillus subtilis

YrrR


2747
Transcription elongation factor greA
UniRef100_P80240

Bacillus subtilis

GreA



[Bacillus subtilis]


2748
Uridine kinase [Bacillus subtilis]
UniRef100_O32033

Bacillus subtilis

Udk


2749
YrrO protein [Bacillus subtilis]
UniRef100_O32034

Bacillus subtilis

YrrO


2750
YrrN protein [Bacillus subtilis]
UniRef100_O32035

Bacillus subtilis

YrrN


2751
YrrM protein [Bacillus subtilis]
UniRef100_O32036

Bacillus subtilis

YrrM


2752
YrrL protein [Bacillus subtilis]
UniRef100_O34758

Bacillus subtilis

YrrL


2753
YrzB protein [Bacillus subtilis]
UniRef100_O34828

Bacillus subtilis



2754
Putative Holliday junction resolvase
UniRef100_O34634

Bacillus subtilis

YrrK



[Bacillus subtilis]


2755
Hypothetical UPF0297 protein yrzL
UniRef100_Q7WY61

Bacillus subtilis




[Bacillus subtilis]


2756
Alanyl-tRNA synthetase [Bacillus
UniRef100_O34526

Bacillus subtilis

AlaS




subtilis]



2757
Hypothetical UPF0118 protein yrrI
UniRef100_O34472

Bacillus subtilis

YrrI



[Bacillus subtilis]


2758


2759
Hypothetical protein [Bacillus cereus
UniRef100_Q634F2

Bacillus cereus ZK




ZK]


2760
YrrD protein [Bacillus subtilis]
UniRef100_O34402

Bacillus subtilis

YrrD


2761
YrrC protein [Bacillus subtilis]
UniRef100_O34481

Bacillus subtilis

YrrC


2762
YrrB protein [Bacillus subtilis]
UniRef100_O34452

Bacillus subtilis

YrrB


2763
Probable tRNA (5-methylaminomethyl-
UniRef100_O35020

Bacillus subtilis

TrmU



2-thiouridylate)-methyltransferase



[Bacillus subtilis]


2764
YrvO protein [Bacillus subtilis]
UniRef100_O34599

Bacillus subtilis

YrvO


2765
BH1259 protein [Bacillus halodurans]
UniRef100_Q9KDF4

Bacillus halodurans



2766
YrvN protein [Bacillus subtilis]
UniRef100_O34528

Bacillus subtilis

YrvN


2767
YrvM protein [Bacillus subtilis]
UniRef100_O32037

Bacillus subtilis

YrvM


2768
Aspartyl-tRNA synthetase [Bacillus
UniRef100_O32038

Bacillus subtilis

AspS




subtilis]



2769
Histidyl-tRNA synthetase [Bacillus
UniRef100_O32039

Bacillus subtilis

HisS




subtilis]



2770


2771



YrvJ


2772
Putative D-tyrosyl-tRNA(Tyr)
UniRef100_O32042

Bacillus subtilis

YrvI



deacylase-like protein [Bacillus subtilis]


2773
GTP pyrophosphokinase (EC 2.7.6.5)
UniRef100_O54408

Bacillus subtilis

RelA



(ATP:GTP 3′-pyrophosphotransferase)



(ppGpp synthetase I) ((P)ppGpp



synthetase) [Bacillus subtilis]


2774
Adenine phosphoribosyltransferase
UniRef100_O34443

Bacillus subtilis

Apt



[Bacillus subtilis]


2775
YrvE protein [Bacillus subtilis]
UniRef100_O32044

Bacillus subtilis

YrvE


2776
YrvD protein [Bacillus subtilis]
UniRef100_O32045

Bacillus subtilis



2777
SecDF protein [Bacillus subtilis]
UniRef100_O32047

Bacillus subtilis

SecDF


2778
YrzD protein [Bacillus subtilis]
UniRef100_O32049

Bacillus subtilis



2779



SpoVB


2780
Hypothetical protein yrbG [Bacillus
UniRef100_O32050

Bacillus subtilis

YrbG




subtilis]



2781
YrzE protein [Bacillus subtilis]
UniRef100_O32051

Bacillus subtilis

YrzE


2782
Hypothetical UPF0092 protein yrbF
UniRef100_O32052

Bacillus subtilis




[Bacillus subtilis]


2783
Queuine tRNA-ribosyltransferase
UniRef100_O32053

Bacillus subtilis

Tgt



[Bacillus subtilis]


2784
S-adenosylmethionine:tRNA
UniRef100_O32054

Bacillus subtilis

QueA



ribosyltransferase-isomerase [Bacillus




subtilis]



2785


2786
Holliday junction DNA helicase ruvB
UniRef100_O32055

Bacillus subtilis

RuvB



[Bacillus subtilis]


2787
Holliday junction DNA helicase ruvA
UniRef100_O05392

Bacillus subtilis

RuvA



[Bacillus subtilis]


2788
BofC protein precursor [Bacillus subtilis]
UniRef100_O05391

Bacillus subtilis

BofC


2789
Hypothetical conserved protein
UniRef100_Q8ERL7

Oceanobacillus

YrzF



[Oceanobacillus iheyensis]


iheyensis



2790


2791
Small, acid-soluble spore protein H
UniRef100_Q9KB75

Bacillus halodurans




[Bacillus halodurans]


2792
Hypothetical protein yjoA [Bacillus
UniRef100_O34334

Bacillus subtilis

YjoA




subtilis]



2793
YmaC protein [Bacillus subtilis]
UniRef100_O31789

Bacillus subtilis

YmaC


2794


2795


2796
Hypothetical UPF0082 protein yrbC
UniRef100_P94447

Bacillus subtilis

YrbC



[Bacillus subtilis]


2797
Sporulation cortex protein coxA
UniRef100_P94446

Bacillus subtilis

CoxA



[Bacillus subtilis]


2798
Morphogenetic protein associated with
UniRef100_O32062

Bacillus subtilis

SafA



SpoVID [Bacillus subtilis]


2799
Quinolinate synthetase A [Bacillus
UniRef100_Q9KWZ1

Bacillus subtilis

NadA




subtilis]



2800
Probable nicotinate-nucleotide
UniRef100_P39666
carboxylating
NadC



pyrophosphorylase [carboxylating]



[Bacillus subtilis]


2801
L-aspartate oxidase [Bacillus subtilis]
UniRef100_P38032

Bacillus subtilis

NadB


2802
Probable cysteine desulfurase [Bacillus
UniRef100_P38033

Bacillus subtilis

NifS




subtilis]



2803



YrxA


2804
Prephenate dehydratase [Bacillus
UniRef100_P21203

Bacillus subtilis

PheA




subtilis]



2805
ACT domain protein pheB [Bacillus
UniRef100_P21204

Bacillus subtilis

PheB




subtilis]



2806
Spo0B-associated GTP-binding protein
UniRef100_Q659J4

Bacillus

Obg



[Bacillus amyloliquefaciens]


amyloliquefaciens



2807
Sporulation initiation
UniRef100_P06535

Bacillus subtilis

Spo0B



phosphotransferase B [Bacillus subtilis]


2808
50S ribosomal protein L27 [Bacillus
UniRef100_P05657

Bacillus subtilis





subtilis]



2809


2810
50S ribosomal protein L21 [Bacillus
UniRef100_P26908

Bacillus subtilis

RplU




subtilis]



2811
Stage IV sporulation protein FB
UniRef100_P26937

Bacillus subtilis

SpoIVFB



[Bacillus subtilis]


2812
Stage IV sporulation protein FA
UniRef100_P26936

Bacillus subtilis

SpoIVFA



[Bacillus subtilis]


2813
Hypothetical protein [Bacillus cereus]
UniRef100_Q816V6

Bacillus cereus

YndB


2814
Transcriptional regulator, ArsR family
UniRef100_Q632Y0

Bacillus cereus ZK




[Bacillus cereus ZK]


2815
Septum site-determining protein minD
UniRef100_Q01464

Bacillus subtilis

MinD



[Bacillus subtilis]


2816



MinC


2817
Rod shape-determining protein mreD
UniRef100_Q01467

Bacillus subtilis

MreD



[Bacillus subtilis]


2818
Rod shape-determining protein mreC
UniRef100_Q01466

Bacillus subtilis

MreC



[Bacillus subtilis]


2819
Rod shape-determining protein mreB
UniRef100_Q01465

Bacillus subtilis

MreB



[Bacillus subtilis]


2820
DNA repair protein radC homolog
UniRef100_Q02170

Bacillus subtilis

RadC



[Bacillus subtilis]


2821
Septum formation protein Maf [Bacillus
UniRef100_Q02169

Bacillus subtilis

Maf




subtilis]



2822
Stage II sporulation protein B [Bacillus
UniRef100_P37575

Bacillus subtilis

SpoIIB




subtilis]



2823
Type 4 prepilin-like proteins leader
UniRef100_P15378
Includes: Leader
ComC



peptide processing enzyme (Late

peptidase (EC



competence protein comC) [Includes:

3.4.23.43)



Leader peptidase (EC 3.4.23.43)

(Prepilin



(Prepilin peptidase); N-

peptidase); N-



methyltransferase (EC 2.1.1.—)] [Bacillus

methyltransferase




subtilis]


(EC 2.1.1.—)


2824



FolC


2825
Valyl-tRNA synthetase [Bacillus subtilis]
UniRef100_Q05873

Bacillus subtilis

ValS


2826
Hypothetical protein OB2062
UniRef100_Q8EPN1

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


2827
Hypothetical protein ysxE [Bacillus
UniRef100_P37964

Bacillus subtilis

YsxE




subtilis]



2828
Stage VI sporulation protein D [Bacillus
UniRef100_P37963

Bacillus subtilis

SpoVID




subtilis]



2829
Glutamate-1-semialdehyde 2,1-
UniRef100_P30949

Bacillus subtilis

HemL



aminomutase [Bacillus subtilis]


2830
Delta-aminolevulinic acid dehydratase
UniRef100_P30950

Bacillus subtilis

HemB



[Bacillus subtilis]


2831
Uroporphyrinogen-III synthase [Bacillus
UniRef100_P21248

Bacillus subtilis

HemD




subtilis]



2832
Porphobilinogen deaminase [Bacillus
UniRef100_P16616

Bacillus subtilis

HemC




subtilis]



2833
Protein hemX [Bacillus subtilis]
UniRef100_P16645

Bacillus subtilis

HemX


2834
Glutamyl-tRNA reductase [Bacillus
UniRef100_P16618

Bacillus subtilis

HemA




subtilis]



2835
Hypothetical protein ysxD [Bacillus
UniRef100_P40736

Bacillus subtilis

YsxD




subtilis]



2836
Probable GTP-binding protein engB
UniRef100_P38424

Bacillus subtilis

YsxC



[Bacillus subtilis]


2837
ATP-dependent protease La 1 [Bacillus
UniRef100_P37945

Bacillus subtilis

LonA




subtilis]



2838
ATP-dependent protease La homolog
UniRef100_P42425

Bacillus subtilis

LonB



[Bacillus subtilis]


2839
ATP-dependent Clp protease ATP-
UniRef100_P50866

Bacillus subtilis

ClpX



binding subunit clpX [Bacillus subtilis]


2840
Trigger factor [Bacillus subtilis]
UniRef100_P80698

Bacillus subtilis

Tig


2841
Hypothetical protein ysoA [Bacillus
UniRef100_P94569

Bacillus subtilis

YsoA




subtilis]



2842
3-isopropylmalate dehydratase small
UniRef100_P94568

Bacillus subtilis

LeuD



subunit [Bacillus subtilis]


2843
3-isopropylmalate dehydratase large
UniRef100_P80858

Bacillus subtilis

LeuC



subunit [Bacillus subtilis]


2844



LeuB


2845
2-isopropylmalate synthase [Bacillus
UniRef100_P94565

Bacillus subtilis

LeuA




subtilis]



2846
Ketol-acid reductoisomerase [Bacillus
UniRef100_P37253

Bacillus subtilis

IlvC




subtilis]



2847
Acetolactate synthase small subunit
UniRef100_P37252

Bacillus subtilis

IlvH



[Bacillus subtilis]


2848
Acetolactate synthase large subunit
UniRef100_P37251

Bacillus subtilis

IlvB



[Bacillus subtilis]


2849
Branched-chain amino acid
UniRef100_Q6HLF7

Bacillus

Dat



aminotransferase [Bacillus


thuringiensis





thuringiensis]



2850


2851



RocG


2852
BH3337 protein [Bacillus halodurans]
UniRef100_Q9K7M4

Bacillus halodurans



2853



YxeD


2854
Hypothetical protein yqbA [Bacillus
UniRef100_P45917

Bacillus subtilis





subtilis]



2855
Hypothetical protein yqaT [Bacillus
UniRef100_P45916

Bacillus subtilis

YqaT




subtilis]



2856
Lin1266 protein [Listeria innocua]
UniRef100_Q92CC3

Listeria innocua



2857
Lin1733 protein [Listeria innocua]
UniRef100_Q92B18

Listeria innocua



2858


2859


2860


2861



MtbP


2862
BH3535 protein [Bacillus halodurans]
UniRef100_Q9K738

Bacillus halodurans



2863



YeeF


2864



YxiD


2865



RapI


2866


2867


2868


2869
Hypothetical UPF0025 protein ysnB
UniRef100_P94559

Bacillus subtilis

YsnB



[Bacillus subtilis]


2870
HAM1 protein homolog [Bacillus
UniRef100_P94558

Bacillus subtilis

YsnA




subtilis]



2871



Rph


2872
Germination protein gerM [Bacillus
UniRef100_P39072

Bacillus subtilis

GerM




subtilis]



2873



RacE


2874
Hypothetical protein ysmB [Bacillus
UniRef100_P97247

Bacillus subtilis

YsmB




subtilis]



2875
Germination protein gerE [Bacillus
UniRef100_P11470

Bacillus subtilis





subtilis]



2876
Oxidoreductase [Clostridium
UniRef100_Q97TP7

Clostridium

YqjQ




acetobutylicum]



acetobutylicum



2877
Hypothetical protein ysmA [Bacillus
UniRef100_Q6L874

Bacillus subtilis

YsmA




subtilis]



2878
Succinate dehydrogenase iron-sulfur
UniRef100_P08066

Bacillus subtilis

SdhB



protein [Bacillus subtilis]


2879



SdhA


2880


2881
Succinate dehydrogenase cytochrome
UniRef100_P08064

Bacillus subtilis

SdhC



B-558 subunit [Bacillus subtilis]


2882
Hypothetical protein yslB [Bacillus
UniRef100_P42955

Bacillus subtilis

YslB




subtilis]



2883
Aspartokinase 2 (EC 2.7.2.4)
UniRef100_P08495
Contains:
LysC



(Aspartokinase II) (Aspartate kinase 2)

Aspartokinase II



[Contains: Aspartokinase II alpha

alpha subunit;



subunit; Aspartokinase II beta subunit]

Aspartokinase II



[Bacillus subtilis]

beta subunit


2884
UvrABC system protein C [Bacillus
UniRef100_P14951

Bacillus subtilis

UvrC




subtilis]



2885
Thioredoxin [Bacillus subtilis]
UniRef100_P14949

Bacillus subtilis

TrxA


2886
Electron transfer flavoprotein alpha-
UniRef100_P94551

Bacillus subtilis

EtfA



subunit [Bacillus subtilis]


2887
Electron transfer flavoprotein beta-
UniRef100_P94550

Bacillus subtilis

EtfB



subunit [Bacillus subtilis]


2888
Hypothetical protein ysiB [Bacillus
UniRef100_P94549

Bacillus subtilis

YsiB




subtilis]



2889
Hypothetical protein ysiA [Bacillus
UniRef100_P94548

Bacillus subtilis

YsiA




subtilis]



2890
Long-chain-fatty-acid--CoA ligase
UniRef100_P94547

Bacillus subtilis

LcfA



[Bacillus subtilis]


2891
Hypothetical protein yshE [Bacillus
UniRef100_P94546

Bacillus subtilis

YshE




subtilis]



2892
MutS2 protein [Bacillus subtilis]
UniRef100_P94545

Bacillus subtilis

MutSB


2893
Hypothetical protein yshC [Bacillus
UniRef100_P94544

Bacillus subtilis

YshC




subtilis]



2894



YshB


2895


2896
Ribonuclease HIII [Bacillus subtilis]
UniRef100_P94541

Bacillus subtilis

RnhC


2897


2898


2899


2900


2901



YxlF


2902


2903
Phenylalanyl-tRNA synthetase beta
UniRef100_P17922

Bacillus subtilis

PheT



chain [Bacillus subtilis]


2904
Phenylalanyl-tRNA synthetase alpha
UniRef100_Q659J3

Bacillus

PheS



chain [Bacillus amyloliquefaciens]


amyloliquefaciens



2905
Hypothetical protein ysgA [Bacillus
UniRef100_P94538

Bacillus subtilis

YsgA




subtilis]



2906
Small, acid-soluble spore protein I
UniRef100_P94537

Bacillus subtilis




[Bacillus subtilis]


2907
Carbon starvation protein A homolog
UniRef100_P94532

Bacillus subtilis

CstA



[Bacillus subtilis]


2908
Alpha-N-arabinofuranosidase [Bacillus
UniRef100_Q9XBQ3

Bacillus

AbfA




stearothermophilus]



stearothermophilus



2909
L-arabinose transport system permease
UniRef100_P94530

Bacillus subtilis

AraQ



protein araQ [Bacillus subtilis]


2910
L-arabinose transport system permease
UniRef100_P94529

Bacillus subtilis

AraP



protein araP [Bacillus subtilis]


2911
Probable arabinose-binding protein
UniRef100_P94528

Bacillus subtilis

AraN



precursor [Bacillus subtilis]


2912
Arabinose operon protein araM
UniRef100_P94527

Bacillus subtilis

AraM



[Bacillus subtilis]


2913
L-ribulose-5-phosphate 4-epimerase
UniRef100_P94525

Bacillus subtilis

AraD



[Bacillus subtilis]


2914
Ribulokinase [Bacillus subtilis]
UniRef100_P94524

Bacillus subtilis

AraB


2915
L-arabinose isomerase [Bacillus
UniRef100_P94523

Bacillus subtilis

AraA




subtilis]



2916



AbnA


2917
Hypothetical protein ysdC [Bacillus
UniRef100_P94521

Bacillus subtilis

YsdC




subtilis]



2918
Hypothetical protein ysdB [Bacillus
UniRef100_P94520

Bacillus subtilis

YsdB




subtilis]



2919
Hypothetical protein ysdA [Bacillus
UniRef100_P94519

Bacillus subtilis





subtilis]



2920
50S ribosomal protein L20 [Bacillus
UniRef100_P55873

Bacillus subtilis

RplT




subtilis]



2921
50S ribosomal protein L35 [Bacillus
UniRef100_P55874

Bacillus subtilis





subtilis]



2922



InfC


2923
Antiholin-like protein IrgB [Bacillus
UniRef100_P94516

Bacillus subtilis

YsbB




subtilis]



2924
Antiholin-like protein IrgA [Bacillus
UniRef100_P94515

Bacillus subtilis

YsbA




subtilis]



2925



PhoD


2926
Sensory transduction protein lytT
UniRef100_P94514

Bacillus subtilis

LytT



[Bacillus subtilis]


2927
Sensor protein lytS [Bacillus subtilis]
UniRef100_P94513

Bacillus subtilis

LytS


2928
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81N00

Bacillus anthracis



2929
Hypothetical protein ysaA [Bacillus
UniRef100_P94512

Bacillus subtilis

YsaA




subtilis]



2930
Threonyl-tRNA synthetase 1 [Bacillus
UniRef100_P18255

Bacillus subtilis

ThrS




subtilis]



2931
Hypothetical protein ytxC [Bacillus
UniRef100_P06569

Bacillus subtilis

YtxC




subtilis]



2932
Hypothetical UPF0043 protein ytxB
UniRef100_P06568

Bacillus subtilis

YtxB



[Bacillus subtilis]


2933
Primosomal protein dnaI [Bacillus
UniRef100_P06567

Bacillus subtilis

DnaI




subtilis]



2934
Replication initiation and membrane
UniRef100_P07908

Bacillus subtilis

DnaB



attachment protein [Bacillus subtilis]


2935
Hypothetical UPF0168 protein ytcG
UniRef100_Q45549

Bacillus subtilis

YtcG



[Bacillus subtilis]


2936


2937



SpeD


2938



GapB


2939
Pectin lyase [Bacillus subtilis]
UniRef100_P94449

Bacillus subtilis

PelB


2940
Dephospho-CoA kinase [Bacillus
UniRef100_O34932

Bacillus subtilis

YtaG




subtilis]



2941



YtaF


2942
Formamidopyrimidine-DNA glycosylase
UniRef100_O34403

Bacillus subtilis

MutM



[Bacillus subtilis]


2943
DNA polymerase I [Bacillus subtilis]
UniRef100_O34996

Bacillus subtilis

PolA


2944
Alkaline phosphatase synthesis sensor
UniRef100_P23545

Bacillus subtilis

PhoR



protein phoR [Bacillus subtilis]


2945
Alkaline phosphatase synthesis
UniRef100_P13792

Bacillus subtilis

PhoP



transcriptional regulatory protein phoP



[Bacillus subtilis]


2946
Malate dehydrogenase [Bacillus
UniRef100_P49814

Bacillus subtilis

Mdh




subtilis]



2947
Isocitrate dehydrogenase [NADP]
UniRef100_P39126
NADP
Icd



[Bacillus subtilis]


2948
Citrate synthase II [Bacillus subtilis]
UniRef100_P39120

Bacillus subtilis

CitZ


2949
YtwI [Bacillus subtilis]
UniRef100_O34811

Bacillus subtilis

YtwI


2950
Hypothetical UPF0118 protein ytvI
UniRef100_O34991

Bacillus subtilis

YtvI



[Bacillus subtilis]


2951
YtzA protein [Bacillus subtilis]
UniRef100_O32064

Bacillus subtilis

YtzA


2952



Pyk


2953
6-phosphofructokinase [Bacillus
UniRef100_O34529

Bacillus subtilis

PfkA




subtilis]



2954
Acetyl-coenzyme A carboxylase
UniRef100_O34847

Bacillus subtilis

AccA



carboxyl transferase subunit alpha



[Bacillus subtilis]


2955
Acetyl-CoA carboxylase subunit
UniRef100_O34571

Bacillus subtilis

AccD



[Bacillus subtilis]


2956



YtsJ


2957
DNA polymerase III alpha subunit
UniRef100_O34623

Bacillus subtilis

DnaE



[Bacillus subtilis]


2958
Hypothetical Membrane Spanning
UniRef100_Q812P3

Bacillus cereus




Protein [Bacillus cereus]


2959
YtrI [Bacillus subtilis]
UniRef100_O34460

Bacillus subtilis

YtrI


2960
BH3172 protein [Bacillus halodurans]
UniRef100_Q9K835

Bacillus halodurans



2961
YtqI [Bacillus subtilis]
UniRef100_O34600

Bacillus subtilis

YtqI


2962
YtpI [Bacillus subtilis]
UniRef100_O34922

Bacillus subtilis



2963



YtoI


2964



PadR


2965
YtkL protein [Bacillus subtilis]
UniRef100_Q795U4

Bacillus subtilis

YtkL


2966



YtkK


2967


2968
Argininosuccinate lyase [Bacillus
UniRef100_O34858

Bacillus subtilis

ArgH




subtilis]



2969
Argininosuccinate synthase [Bacillus
UniRef100_O34347

Bacillus subtilis

ArgG




subtilis]



2970
Molybdenum cofactor biosynthesis
UniRef100_O34457

Bacillus subtilis

MoaB



protein B [Bacillus subtilis]


2971



AckA


2972
Hypothetical protein ytxK [Bacillus
UniRef100_P37876

Bacillus subtilis

YtxK




subtilis]



2973
Probable thiol peroxidase [Bacillus
UniRef100_P80864

Bacillus subtilis

Tpx




subtilis]



2974
YtfJ [Bacillus subtilis]
UniRef100_O34806

Bacillus subtilis

YtfJ


2975



YtfI


2976
YteJ [Bacillus subtilis]
UniRef100_O34424

Bacillus subtilis

YteJ


2977
Putative signal peptide peptidase sppA
UniRef100_O34525

Bacillus subtilis

SppA



[Bacillus subtilis]


2978
Probable inorganic polyphosphate/ATP-
UniRef100_O34934

Bacillus subtilis

YtdI



NAD kinase 2 (EC 2.7.1.23)



(Poly(P)/ATP NAD kinase 2) [Bacillus




subtilis]



2979
YhbJ protein [Bacillus subtilis]
UniRef100_O31593

Bacillus subtilis

YhbJ


2980
Multidrug resistance protein
UniRef100_Q8CQB1

Staphylococcus

YubD



[Staphylococcus epidermidis]


epidermidis



2981
Putative HTH-type transcriptional
UniRef100_P42103

Bacillus subtilis

YxaD



regulator yxaD [Bacillus subtilis]


2982
YtcI [Bacillus subtilis]
UniRef100_O34613

Bacillus subtilis

YtcI


2983
Small, acid-soluble spore protein 1
UniRef100_P06552

Bacillus stearothermophilus




[Bacillus stearothermophilus]


2984
Probable thiamine biosynthesis protein
UniRef100_O34595

Bacillus subtilis

YtbJ



thil [Bacillus subtilis]


2985
NifS2 [Bacillus subtilis]
UniRef100_O34874

Bacillus subtilis

NifZ


2986
Branched-chain amino acid transport
UniRef100_O34545

Bacillus subtilis

BraB



system carrier protein braB [Bacillus




subtilis]



2987
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012





anthracis str. A2012]



2988
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069


2989
Septation ring formation regulator ezrA
UniRef100_O34894

Bacillus subtilis

EzrA



[Bacillus subtilis]


2990
Histidinol-phosphatase [Bacillus
UniRef100_O34411

Bacillus subtilis

HisJ




subtilis]



2991
Probable HTH-type transcriptional
UniRef100_O34970

Bacillus subtilis

YttP



regulator yttP [Bacillus subtilis]


2992
Hypothetical conserved protein
UniRef100_Q8EPB0

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


2993
YtrP [Bacillus subtilis]
UniRef100_O34325

Bacillus subtilis

YtrP


2994
30S ribosomal protein S4 [Bacillus
UniRef100_P21466

Bacillus subtilis

RpsD




subtilis]



2995


2996


2997



YddR


2998
HTH-type transcriptional regulator lrpA
UniRef100_P96652

Bacillus subtilis

LrpA



[Bacillus subtilis]


2999


3000
Tyrosyl-tRNA synthetase 1 [Bacillus
UniRef100_P22326

Bacillus subtilis

TyrS




subtilis]



3001
Acetyl-coenzyme A synthetase [Bacillus
UniRef100_P39062

Bacillus subtilis

AcsA




subtilis]



3002
Acetoin utilization protein acuA [Bacillus
UniRef100_P39065

Bacillus subtilis

AcuA




subtilis]



3003
Acetoin utilization acuB protein [Bacillus
UniRef100_P39066

Bacillus subtilis

AcuB




subtilis]



3004
Acetoin utilization protein acuC
UniRef100_P39067

Bacillus subtilis

AcuC



[Bacillus subtilis]


3005
Hypothetical protein ytxE [Bacillus
UniRef100_P39064

Bacillus subtilis

YtxE




subtilis]



3006
Hypothetical protein ytxD [Bacillus
UniRef100_P39063

Bacillus subtilis

YtxD




subtilis]



3007
Catabolite control protein A [Bacillus
UniRef100_P25144

Bacillus subtilis

CcpA




subtilis]



3008
AroA(G) protein [Includes: Phospho-2-
UniRef100_P39912
Includes:
AroA



dehydro-3-deoxyheptonate aldolase

Phospho-2-



(EC 2.5.1.54) (Phospho-2-keto-3-

dehydro-3-



deoxyheptonate aldolase) (DAHP

deoxyheptonate



synthetase) (3-deoxy-D-arabino-

aldolase (EC



heptulosonate 7-phosphate synthase);

2.5.1.54)



Chorismate mutase (EC 5.4.99.5)]

(Phospho-2-keto-



[Bacillus subtilis]

3-





deoxyheptonate





aldolase) (DAHP





synthetase) (3-





deoxy-D-arabino-





heptulosonate 7-





phosphate





synthase);





Chorismate





mutase (EC





5.4.99.5)


3009
Similar to hypothetical repeat
UniRef100_Q7N3B8

Photorhabdus luminescens




containing protein [Photorhabdus




luminescens]



3010
Hypothetical protein ytxJ [Bacillus
UniRef100_P39914

Bacillus subtilis

YtxJ




subtilis]



3011
Hypothetical protein ytxH [Bacillus
UniRef100_P40780

Bacillus subtilis

YtxH




subtilis]



3012
Hypothetical protein ytxG [Bacillus
UniRef100_P40779

Bacillus subtilis

YtxG




subtilis]



3013



MurC


3014
YtpT [Bacillus subtilis]
UniRef100_O34749

Bacillus subtilis

YtpT


3015
YtpR [Bacillus subtilis]
UniRef100_O34943

Bacillus subtilis

YtpR


3016
YtpQ [Bacillus subtilis]
UniRef100_O34496

Bacillus subtilis

YtpQ


3017
Putative thioredoxin [Bacillus subtilis]
UniRef100_O34357

Bacillus subtilis

YtpP


3018
YtoQ [Bacillus subtilis]
UniRef100_O34305

Bacillus subtilis

YtoQ


3019
YtoP [Bacillus subtilis]
UniRef100_O34924

Bacillus subtilis

YtoP


3020
YtzB protein [Bacillus subtilis]
UniRef100_O32065

Bacillus subtilis

YtzB


3021
Probable NAD-dependent malic
UniRef100_O34389

Bacillus subtilis

MalS



enzyme 3 [Bacillus subtilis]


3022
YtnP [Bacillus subtilis]
UniRef100_O34760

Bacillus subtilis

YtnP


3023
tRNA (guanine-N(7)-)-
UniRef100_O34522

Bacillus subtilis

YtmQ



methyltransferase (EC 2.1.1.33)



(tRNA(m7G46)-methyltransferase)



[Bacillus subtilis]


3024
YtzH protein [Bacillus subtilis]
UniRef100_O32066

Bacillus subtilis



3025
YtmP [Bacillus subtilis]
UniRef100_O34935

Bacillus subtilis

YtmP


3026
AmyX protein [Bacillus subtilis]
UniRef100_O34587

Bacillus subtilis

AmyX


3027
YtlR [Bacillus subtilis]
UniRef100_O34799

Bacillus subtilis

YtlR


3028
YtlQ [Bacillus subtilis]
UniRef100_O34471

Bacillus subtilis

YtlQ


3029
Hypothetical UPF0097 protein ytlP
UniRef100_O34570

Bacillus subtilis

YtlP



[Bacillus subtilis]


3030
Probable cysteine synthase (EC
UniRef100_O34476

Bacillus subtilis

YtkP



2.5.1.47) (O-acetylserine sulfhydrylase)



(O-acetylserine (Thiol)-lyase) [Bacillus




subtilis]



3031
Hypothetical protein [Bacillus cereus]
UniRef100_Q81BR8

Bacillus cereus

YncE


3032
Putative peptidase [Bacillus subtilis]
UniRef100_O34944

Bacillus subtilis

YtjP


3033
YtiP [Bacillus subtilis]
UniRef100_O34978

Bacillus subtilis

YtiP


3034
YtzE protein [Bacillus subtilis]
UniRef100_O32067

Bacillus subtilis



3035
Ribosomal small subunit pseudouridine
UniRef100_Q816W1

Bacillus cereus

YtzF



synthase A [Bacillus cereus]


3036
YtgP [Bacillus subtilis]
UniRef100_O34674

Bacillus subtilis

YtgP


3037
YtfP [Bacillus subtilis]
UniRef100_O30505

Bacillus subtilis

YtfP


3038



OpuD


3039
Protein cse60 [Bacillus subtilis]
UniRef100_P94496

Bacillus subtilis



3040
Rhodanese-like domain protein
UniRef100_Q72YZ9

Bacillus cereus




[Bacillus cereus]


3041



RapA


3042
Hypothetical protein [Bacillus
UniRef100_Q6HI31

Bacillus thuringiensis





thuringiensis]



3043
YteU [Bacillus subtilis]
UniRef100_O34378

Bacillus subtilis

YteU


3044



YteT


3045



YteS


3046
YteR [Bacillus subtilis]
UniRef100_O34559

Bacillus subtilis

YteR


3047
Transmembrane lipoprotein [Bacillus
UniRef100_Q9KFJ5

Bacillus

LplB




halodurans]



halodurans



3048
YtdP protein [Bacillus subtilis]
UniRef100_O32071

Bacillus subtilis

YtdP


3049
YtcQ protein [Bacillus subtilis]
UniRef100_Q795R2

Bacillus subtilis

YtcQ


3050



YtcP


3051
Hypothetical protein ytbQ [Bacillus
UniRef100_P53560

Bacillus subtilis

YtbQ




subtilis]



3052
YtaP [Bacillus subtilis]
UniRef100_O34973

Bacillus subtilis

YtaP


3053
Amino acid/polyamine transporter;
UniRef100_Q6LYX9

Methanococcus

YecA



family I [Methanococcus maripaludis]


maripaludis



3054
Transcriptional regulator, LysR family
UniRef100_Q97DX1

Clostridium

YwqM



[Clostridium acetobutylicum]


acetobutylicum



3055
Prolyl endopeptidase [Bacillus cereus]
UniRef100_Q81C54

Bacillus cereus

YycE


3056
Leucyl-tRNA synthetase [Bacillus
UniRef100_P36430

Bacillus subtilis

LeuS




subtilis]



3057



YtvB


3058
YttB [Bacillus subtilis]
UniRef100_O34546

Bacillus subtilis

YttB


3059
Lipoprotein [Oceanobacillus iheyensis]
UniRef100_Q8EPK3

Oceanobacillus

YusA






iheyensis



3060
YttA [Bacillus subtilis]
UniRef100_O30500

Bacillus subtilis

YttA


3061
YtrF [Bacillus subtilis]
UniRef100_O35005

Bacillus subtilis

YtrF


3062
Hypothetical ABC transporter ATP-
UniRef100_O34392

Bacillus subtilis

YtrE



binding protein ytrE [Bacillus subtilis]


3063
YtrC [Bacillus subtilis]
UniRef100_O34898

Bacillus subtilis

YtrC


3064
Transporter [Bacillus subtilis]
UniRef100_O34641

Bacillus subtilis

YtrB


3065
Transcription regulator [Bacillus subtilis]
UniRef100_O34712

Bacillus subtilis

YtrA


3066
Hypothetical protein ytzC [Bacillus
UniRef100_O32073

Bacillus subtilis





subtilis]



3067
YtqA [Bacillus subtilis]
UniRef100_O35008

Bacillus subtilis

YtqA


3068



YtqB


3069
Proton glutamate symport protein
UniRef100_P39817

Bacillus subtilis

GltP



[Bacillus subtilis]


3070
Hypothetical protein ytpB [Bacillus
UniRef100_O34707

Bacillus subtilis

YtpB




subtilis]



3071
Probable lysophospholipase [Bacillus
UniRef100_O34705

Bacillus subtilis

YtpA




subtilis]



3072
YtoA [Bacillus subtilis]
UniRef100_O34696

Bacillus subtilis

YtoA


3073



YwoA


3074
Glycosyl transferase, group 1 family
UniRef100_Q6HCB9

Bacillus

TuaC



[Bacillus thuringiensis]


thuringiensis



3075
Asparagine synthetase [glutamine-
UniRef100_P54420
glutamine-
AsnB



hydrolyzing] 1 [Bacillus subtilis]

hydrolyzing


3076
S-adenosylmethionine synthetase
UniRef100_P54419

Bacillus subtilis

MetK



[Bacillus subtilis]


3077
Phosphoenolpyruvate carboxykinase
UniRef100_P54418
ATP
PckA



[ATP] [Bacillus subtilis]


3078
Sodium:dicarboxylate symporter
UniRef100_Q8EP16

Oceanobacillus

DctP



[Oceanobacillus iheyensis]


iheyensis



3079
Hypothetical protein ytmB [Bacillus
UniRef100_O34365

Bacillus subtilis





subtilis]



3080
Putative peptidase [Bacillus subtilis]
UniRef100_O34493

Bacillus subtilis

YtmA


3081
ABC transporter substrate-binding
UniRef100_Q816P5

Bacillus cereus

YtlA



protein [Bacillus cereus]


3082
Putative transporter [Bacillus subtilis]
UniRef100_O34314

Bacillus subtilis

YtlC


3083



YtlD


3084
YtkD [Bacillus subtilis]
UniRef100_O35013

Bacillus subtilis

YtkD


3085
Hypothetical protein [Bacillus
UniRef100_Q6HC91

Bacillus thuringiensis





thuringiensis]



3086
Hypothetical protein ytkC [Bacillus
UniRef100_O34883

Bacillus subtilis

YtkC




subtilis]



3087
General stress protein 20U [Bacillus
UniRef100_P80879

Bacillus subtilis

Dps




subtilis]



3088
Hypothetical protein ytkA [Bacillus
UniRef100_P40768

Bacillus subtilis

YtkA




subtilis]



3089
S-ribosylhomocysteinase [Bacillus
UniRef100_O34667

Bacillus subtilis

LuxS




subtilis]



3090
Hypothetical UPF0161 protein ytjA
UniRef100_O34601

Bacillus subtilis




[Bacillus subtilis]


3091
YtiB [Bacillus subtilis]
UniRef100_O34872

Bacillus subtilis

YtiB


3092
Low-affinity zinc transport protein
UniRef100_Q81F90

Bacillus cereus

YciC



[Bacillus cereus]


3093
High-affinity zinc uptake system protein
UniRef100_Q81EF8

Bacillus cereus

YcdH



znuA [Bacillus cereus]


3094
50S ribosomal protein L31 type B
UniRef100_O34967

Bacillus subtilis




[Bacillus subtilis]


3095
YthA [Bacillus subtilis]
UniRef100_O34655

Bacillus subtilis

YthA


3096
YthB [Bacillus subtilis]
UniRef100_O34505

Bacillus subtilis

YthB


3097
Hypothetical protein [Bacillus cereus]
UniRef100_Q737J1

Bacillus cereus



3098


3099
O-succinylbenzoate synthase (EC
UniRef100_O34514

Bacillus subtilis

MenC



4.2.1.—) (OSB synthase) (OSBS) (4-(2′-



carboxyphenyl)-4-oxybutyric acid



synthase) [Bacillus subtilis]


3100
O-succinylbenzoate--CoA ligase
UniRef100_P23971

Bacillus subtilis

MenE



[Bacillus subtilis]


3101
Naphthoate synthase [Bacillus subtilis]
UniRef100_P23966

Bacillus subtilis

MenB


3102



YtxM


3103
Menaquinone biosynthesis protein
UniRef100_P23970
Includes: 2-
MenD



menD [Includes: 2-succinyl-6-hydroxy-

succinyl-6-



2,4-cyclohexadiene-1-carboxylate

hydroxy-2,4-



synthase (EC 2.5.1.64) (SHCHC

cyclohexadiene-



synthase); 2-oxoglutarate

1-carboxylate



decarboxylase (EC 4.1.1.71) (Alpha-

synthase (EC



ketoglutarate decarboxylase) (KDC)]

2.5.1.64)



[Bacillus subtilis]

(SHCHC





synthase); 2-





oxoglutarate





decarboxylase





(EC 4.1.1.71)





(Alpha-





ketoglutarate





decarboxylase)





(KDC)


3104
Menaquinone-specific isochorismate
UniRef100_P23973

Bacillus subtilis

MenF



synthase [Bacillus subtilis]


3105
Probable 1,4-dihydroxy-2-naphthoate
UniRef100_P39582

Bacillus subtilis

MenA



octaprenyltransferase [Bacillus subtilis]


3106
Hypothetical protein yteA [Bacillus
UniRef100_P42408

Bacillus subtilis

YteA




subtilis]



3107
Glycogen phosphorylase [Bacillus
UniRef100_P39123

Bacillus subtilis

GlgP




subtilis]



3108
Glycogen synthase [Bacillus subtilis]
UniRef100_P39125

Bacillus subtilis

GlgA


3109
Glycogen biosynthesis protein glgD
UniRef100_P39124

Bacillus subtilis

GlgD



[Bacillus subtilis]


3110
Glucose-1-phosphate
UniRef100_P39122

Bacillus subtilis

GlgC



adenylyltransferase [Bacillus subtilis]


3111
1,4-alpha-glucan branching enzyme
UniRef100_P39118

Bacillus subtilis

GlgB



[Bacillus subtilis]


3112



AraR


3113
YuaJ protein [Bacillus subtilis]
UniRef100_O32074

Bacillus subtilis

YuaJ


3114
BH4010 protein [Bacillus halodurans]
UniRef100_Q9K5S8

Bacillus

YhcS






halodurans



3115
BH4011 protein [Bacillus halodurans]
UniRef100_Q9K5S7

Bacillus halodurans



3116


3117



RapD


3118
Pyrrolidone-carboxylate peptidase
UniRef100_P46107

Bacillus

Pcp



[Bacillus amyloliquefaciens]


amyloliquefaciens



3119
BH0597 protein [Bacillus halodurans]
UniRef100_Q9KF88

Bacillus

YuaA






halodurans



3120



YubG


3121



YxxF


3122
YuaE protein [Bacillus subtilis]
UniRef100_O32078

Bacillus subtilis

YuaE


3123
YuaD protein [Bacillus subtilis]
UniRef100_O32079

Bacillus subtilis

YuaD


3124
Alcohol dehydrogenase [Bacillus
UniRef100_P71017

Bacillus subtilis

GbsB




subtilis]



3125
Betaine aldehyde dehydrogenase
UniRef100_P71016

Bacillus subtilis

GbsA



[Bacillus subtilis]


3126
Hypothetical protein yuaC [Bacillus
UniRef100_P71015

Bacillus subtilis

YuaC




subtilis]



3127
UPI00002D3D35 UniRef100 entry
UniRef100_UPI00002D3D35

OpuE


3128
Hypothetical protein yktD [Bacillus
UniRef100_Q45500

Bacillus subtilis

YktD




subtilis]



3129
Alanine racemase 2 [Bacillus subtilis]
UniRef100_P94494

Bacillus subtilis

YncD


3130
Oxalate decarboxylase [Bacillus
UniRef100_Q81GZ6

Bacillus cereus

YoaN




cereus]



3131
Hypothetical protein CAC0135
UniRef100_Q97MQ7

Clostridium acetobutylicum




[Clostridium acetobutylicum]


3132
Hypothetical protein [Bacillus
UniRef100_Q6HGC9

Bacillus thuringiensis





thuringiensis]



3133
Hypothetical protein [Bacillus
UniRef100_Q6HGC8

Bacillus thuringiensis





thuringiensis]



3134


3135
Hypothetical protein [Bacillus
UniRef100_Q6HGC6

Bacillus thuringiensis





thuringiensis]



3136
Hypothetical protein [Bacillus
UniRef100_Q6HGC5

Bacillus thuringiensis





thuringiensis]



3137
Hypothetical conserved protein
UniRef100_Q8ETF5

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


3138


3139


3140
YkoN [Bacillus subtilis]
UniRef100_O34625

Bacillus subtilis

YkoN


3141
Hypothetical protein ykoP [Bacillus
UniRef100_O34495

Bacillus subtilis

YkoP




subtilis]



3142
Hypothetical UPF0151 protein ykoQ
UniRef100_O35040

Bacillus subtilis

YkoQ



[Bacillus subtilis]


3143
Undecaprenyl-diphosphatase [Bacillus
UniRef100_P94507

Bacillus subtilis

YubB




subtilis]



3144
Hypothetical UPF0118 protein yubA
UniRef100_O32086

Bacillus subtilis

YubA



[Bacillus subtilis]


3145
Hypothetical oxidoreductase yulF
UniRef100_O05265

Bacillus subtilis

YulF



[Bacillus subtilis]


3146
Lmo2256 protein [Listeria
UniRef100_Q929B9

Listeria

YraA




monocytogenes]



monocytogenes



3147



McpA


3148



McpA


3149



McpA


3150
Protein-glutamine gamma-
UniRef100_P40746

Bacillus subtilis

Tgl



glutamyltransferase [Bacillus subtilis]


3151
2-nitropropane dioxygenase [Bacillus
UniRef100_O05413

Bacillus subtilis

YrpB




subtilis]



3152
Hypothetical UPF0047 protein yugU
UniRef100_O05243

Bacillus subtilis

YugU



[Bacillus subtilis]


3153
Hypothetical protein yugT [Bacillus
UniRef100_O05242

Bacillus subtilis

YugT




subtilis]



3154
Transcriptional regulator, TetR family
UniRef100_Q81GX6

Bacillus cereus

YfiR



[Bacillus cereus]


3155
Hypothetical protein yqeB [Bacillus
UniRef100_P54447

Bacillus subtilis

YqeB




subtilis]



3156
Beta(1,4)-glucan glucanohydrolase
UniRef100_Q6D3B7

Erwinia carotovora




[Erwinia carotovora]


3157
Hypothetical UPF0053 protein yugS
UniRef100_O05241

Bacillus subtilis

YugS



[Bacillus subtilis]


3158
Hypothetical protein yugP [Bacillus
UniRef100_O05248

Bacillus subtilis

YugP




subtilis]



3159
YugO protein [Bacillus subtilis]
UniRef100_Q795M8

Bacillus subtilis



3160
Hypothetical protein yugN [Bacillus
UniRef100_O05246

Bacillus subtilis

YugN




subtilis]



3161
Hypothetical protein [Bacillus
UniRef100_Q6HIW1

Bacillus

YdfR




thuringiensis]



thuringiensis



3162
YtaB protein [Bacillus subtilis]
UniRef100_O34694

Bacillus subtilis

YtaB


3163
Predicted acetyltransferase [Clostridium
UniRef100_Q97IT3

Clostridium

YkkB




acetobutylicum]



acetobutylicum



3164
Glucose-6-phosphate isomerase
UniRef100_P80860

Bacillus subtilis

Pgi



[Bacillus subtilis]


3165
Probable NADH-dependent butanol
UniRef100_O05239

Bacillus subtilis

YugJ



dehydrogenase 1 [Bacillus subtilis]


3166
YuzA protein [Bacillus subtilis]
UniRef100_O32087

Bacillus subtilis



3167
General stress protein 13 [Bacillus
UniRef100_P80870

Bacillus subtilis

YugI




subtilis]



3168
Alanine transaminase [Bacillus subtilis]
UniRef100_Q795M6

Bacillus subtilis

AlaT


3169
Transcriptional regulator [Bacillus
UniRef100_O05236

Bacillus subtilis

AlaR




subtilis]



3170
Hypothetical protein yugF [Bacillus
UniRef100_O05235

Bacillus subtilis

YugF




subtilis]



3171
Hypothetical protein yugE [Bacillus
UniRef100_O05234

Bacillus subtilis





subtilis]



3172
Hypothetical protein SMU.305
UniRef100_Q9X669

Streptococcus mutans




[Streptococcus mutans]


3173
Putative aminotransferase B [Bacillus
UniRef100_Q08432

Bacillus subtilis

PatB




subtilis]



3174


3175
Kinase-associated lipoprotein B
UniRef100_Q08429

Bacillus subtilis

KapB



precursor [Bacillus subtilis]


3176
Hypothetical protein yugB [Bacillus
UniRef100_O05231

Bacillus subtilis

KapD




subtilis]



3177



YuxJ


3178



PbpD


3179
Hypothetical protein yuxK [Bacillus
UniRef100_P40761

Bacillus subtilis

YuxK




subtilis]



3180
Hypothetical protein yufK [Bacillus
UniRef100_O05249

Bacillus subtilis

YufK




subtilis]



3181
Hypothetical protein yufL [Bacillus
UniRef100_O05250

Bacillus subtilis

YufL




subtilis]



3182
Hypothetical protein yufM [Bacillus
UniRef100_O05251

Bacillus subtilis

YufM




subtilis]



3183


3184
UPI00003CB938 UniRef100 entry
UniRef100_UPI00003CB938

PssA


3185
Hypothetical protein ybfM [Bacillus
UniRef100_O31453

Bacillus subtilis

YbfM




subtilis]



3186
Phosphatidylserine decarboxylase
UniRef100_Q6HDI5

Bacillus

Psd



[Bacillus thuringiensis]


thuringiensis



3187
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069


3188
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012





anthracis str. A2012]



3189
Na(+)-malate symporter [Bacillus
UniRef100_O05256

Bacillus subtilis

MaeN




subtilis]



3190


3191
Na(+)/H(+) antiporter subunit A
UniRef100_Q9K2S2

Bacillus subtilis

MrpA



[Bacillus subtilis]


3192
Na(+)/H(+) antiporter subunit B
UniRef100_O05259

Bacillus subtilis

MrpB



[Bacillus subtilis]


3193
Na(+)/H(+) antiporter subunit C
UniRef100_O05260

Bacillus subtilis

MrpC



[Bacillus subtilis]


3194
Na(+)/H(+) antiporter subunit D
UniRef100_O05229

Bacillus subtilis

MrpD



[Bacillus subtilis]


3195
Na(+)/H(+) antiporter subunit E
UniRef100_Q7WY60

Bacillus subtilis

MrpE



[Bacillus subtilis]


3196
Na(+)/H(+) antiporter subunit F
UniRef100_O05228

Bacillus subtilis




[Bacillus subtilis]


3197
Na(+)/H(+) antiporter subunit G
UniRef100_O05227

Bacillus subtilis

MrpG



[Bacillus subtilis]


3198



YuxO


3199



ComA


3200



ComP


3201
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012





anthracis str. A2012]



3202
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069


3203



ComP


3204


3205
ComQ [Bacillus subtilis]
UniRef100_Q9K5L3

Bacillus subtilis

ComQ


3206


3207
Hypothetical protein yuzC [Bacillus
UniRef100_O32089

Bacillus subtilis

YuzC




subtilis]



3208
Hypothetical protein yuxH [Bacillus
UniRef100_P14203

Bacillus subtilis

YuxH




subtilis]



3209
YueK protein [Bacillus subtilis]
UniRef100_O32090

Bacillus subtilis

YueK


3210
YueJ protein [Bacillus subtilis]
UniRef100_O32091

Bacillus subtilis

YueJ


3211



YueI


3212


3213
Hypothetical protein yueG [Bacillus
UniRef100_O32094

Bacillus subtilis





subtilis]



3214



YueF


3215
RRF2 family protein [Bacillus cereus]
UniRef100_Q81EX1

Bacillus cereus

YwnA


3216
Probable lipase/esterase
UniRef100_Q7UUA2

Rhodopirellula

YuxL



[Rhodopirellula baltica]


baltica



3217
BH1896 protein [Bacillus halodurans]
UniRef100_Q9KBN0

Bacillus halodurans



3218
YueE protein [Bacillus subtilis]
UniRef100_O32098

Bacillus subtilis

YueE


3219
YueD protein [Bacillus subtilis]
UniRef100_O32099

Bacillus subtilis

YueD


3220
Hypothetical protein yueC [Bacillus
UniRef100_O32100

Bacillus subtilis

YueC




subtilis]



3221
YueB protein [Bacillus subtilis]
UniRef100_O32101

Bacillus subtilis

YueB


3222
YukA protein [Bacillus subtilis]
UniRef100_P71068

Bacillus subtilis

YukA


3223
YukC protein [Bacillus subtilis]
UniRef100_P71070

Bacillus subtilis

YukC


3224
YukD protein [Bacillus subtilis]
UniRef100_P71071

Bacillus subtilis



3225
Lin0049 protein [Listeria innocua]
UniRef100_Q92FQ4

Listeria innocua



3226



YflA


3227
YukF protein [Bacillus subtilis]
UniRef100_P71073

Bacillus subtilis

YukF


3228
Alanine dehydrogenase [Bacillus
UniRef100_Q08352

Bacillus subtilis

Ald




subtilis]



3229


3230
YuiH protein [Bacillus subtilis]
UniRef100_O32103

Bacillus subtilis

YuiH


3231
YuiG protein [Bacillus subtilis]
UniRef100_O32104

Bacillus subtilis

YuiG


3232
YuiF protein [Bacillus subtilis]
UniRef100_O32105

Bacillus subtilis

YuiF


3233
Probable cytosol aminopeptidase
UniRef100_O32106

Bacillus subtilis

YuiE



[Bacillus subtilis]


3234
YuiD protein [Bacillus subtilis]
UniRef100_O32107

Bacillus subtilis

YuiD


3235
YuiC protein [Bacillus subtilis]
UniRef100_O32108

Bacillus subtilis

YuiC


3236
YuiB protein [Bacillus subtilis]
UniRef100_O32109

Bacillus subtilis

YuiB


3237


3238



YumB


3239
Thioredoxine reductase [Bacillus
UniRef100_O05268

Bacillus subtilis

YumC




subtilis]



3240


3241
YdjO protein [Bacillus subtilis]
UniRef100_O34759

Bacillus subtilis



3242



YxbD


3243
Hypothetical protein yutM [Bacillus
UniRef100_O32113

Bacillus subtilis

YutM




subtilis]



3244
Diaminopimelate epimerase [Bacillus
UniRef100_O32114

Bacillus subtilis

DapF




subtilis]



3245
YutK protein [Bacillus subtilis]
UniRef100_O32115

Bacillus subtilis

YutK


3246
YuzB protein [Bacillus subtilis]
UniRef100_O32116

Bacillus subtilis



3247
YutJ protein [Bacillus subtilis]
UniRef100_O32117

Bacillus subtilis

YutJ


3248
YdhG protein [Bacillus subtilis]
UniRef100_O05499

Bacillus subtilis

YdhG


3249
Response regulator aspartate
UniRef100_Q9KBE1

Bacillus

RapI



phosphatase [Bacillus halodurans]


halodurans



3250
Phenolic acid decarboxylase [Bacillus
UniRef100_O07006

Bacillus subtilis

PadC




subtilis]



3251
BH2266 protein [Bacillus halodurans]
UniRef100_Q9KAM1

Bacillus halodurans



3252
YuzD protein [Bacillus subtilis]
UniRef100_O32118

Bacillus subtilis

YuzD


3253
YutI protein [Bacillus subtilis]
UniRef100_O32119

Bacillus subtilis



3254
Probable peptidase yuxL [Bacillus
UniRef100_P39839

Bacillus subtilis

YuxL




subtilis]



3255
Homoserine kinase [Bacillus subtilis]
UniRef100_P04948

Bacillus subtilis

ThrB


3256
Threonine synthase [Bacillus subtilis]
UniRef100_P04990

Bacillus subtilis

ThrC


3257
Homoserine dehydrogenase [Bacillus
UniRef100_P19582

Bacillus subtilis

Hom




subtilis]



3258
Glycerate dehydrogenase
UniRef100_Q8ENW9

Oceanobacillus

YvcT



[Oceanobacillus iheyensis]


iheyensis



3259
YutH protein [Bacillus subtilis]
UniRef100_O32123

Bacillus subtilis

YutH


3260
Hypothetical protein yutG [Bacillus
UniRef100_O32124

Bacillus subtilis

YutG




subtilis]



3261
YutF protein [Bacillus subtilis]
UniRef100_O32125

Bacillus subtilis

YutF


3262
YutE protein [Bacillus subtilis]
UniRef100_O32126

Bacillus subtilis

YutE


3263
YutD protein [Bacillus subtilis]
UniRef100_O32127

Bacillus subtilis



3264
YutC protein [Bacillus subtilis]
UniRef100_O32128

Bacillus subtilis

YutC


3265



LipA


3266
YunA protein [Bacillus subtilis]
UniRef100_O32130

Bacillus subtilis

YunA


3267


3268
Sodium-dependent transporter [Bacillus
UniRef100_Q9K7C5

Bacillus

YocR




halodurans]



halodurans



3269
YunB protein [Bacillus subtilis]
UniRef100_O32131

Bacillus subtilis

YunB


3270
YunC protein [Bacillus subtilis]
UniRef100_O32132

Bacillus subtilis

YunC


3271
YunD protein [Bacillus subtilis]
UniRef100_O32133

Bacillus subtilis

YunD


3272
YunE protein [Bacillus subtilis]
UniRef100_O32134

Bacillus subtilis

YunE


3273
YunF protein [Bacillus subtilis]
UniRef100_O32135

Bacillus subtilis

YunF


3274



YmcC


3275
TetR family transcriptional regulator?
UniRef100_Q67KA4

Symbiobacterium

PksA



[Symbiobacterium thermophilum]


thermophilum



3276


3277
Purine catabolism protein pucG
UniRef100_O32148

Bacillus subtilis

YurG



[Bacillus subtilis]


3278
Allantoate amidohydrolase [Bacillus
UniRef100_O32149

Bacillus subtilis

YurH




subtilis]



3279
Purine catabolism regulatory protein
UniRef100_O32138

Bacillus subtilis

PucR



[Bacillus subtilis]


3280
Multidrug resistance protein B [Bacillus
UniRef100_Q63FH7

Bacillus cereus

Blt



cereus ZK]

ZK


3281
BH2308 protein [Bacillus halodurans]
UniRef100_Q9KAH9

Bacillus

YcgA






halodurans



3282



TrpD


3283
Anthranilate phosphoribosyltransferase
UniRef100_Q8U089

Pyrococcus furiosus




[Pyrococcus furiosus]


3284
Extracellular ribonuclease precursor
UniRef100_O32150

Bacillus subtilis

YurI



[Bacillus subtilis]


3285
BH1977 protein [Bacillus halodurans]
UniRef100_Q9KBF1

Bacillus halodurans



3286



YurR


3287
Putative membrane protein [Bordetella
UniRef100_Q7WGW7

Bordetella bronchiseptica





bronchiseptica]



3288
UPI00003CB453 UniRef100 entry
UniRef100_UPI00003CB453


3289
Response regulator aspartate
UniRef100_P96649

Bacillus subtilis

RapI



phosphatase I [Bacillus subtilis]


3290
YurU protein [Bacillus subtilis]
UniRef100_O32162

Bacillus subtilis

YurU


3291
NifU-like protein [Bacillus subtilis]
UniRef100_O32163

Bacillus subtilis

YurV


3292
Probable cysteine desulfurase [Bacillus
UniRef100_O32164

Bacillus subtilis

Csd




subtilis]



3293
YurX protein [Bacillus subtilis]
UniRef100_O32165

Bacillus subtilis

YurX


3294
Vegetative protein 296 [Bacillus subtilis]
UniRef100_P80866

Bacillus subtilis

YurY


3295
Lmo2575 protein [Listeria
UniRef100_Q8Y480

Listeria

CzcD




monocytogenes]



monocytogenes



3296


3297
BH3473 protein [Bacillus halodurans]
UniRef100_Q9K796

Bacillus

YurZ






halodurans



3298
YusA protein [Bacillus subtilis]
UniRef100_O32167

Bacillus subtilis

YusA


3299
YusB protein [Bacillus subtilis]
UniRef100_O32168

Bacillus subtilis

YusB


3300
YusC protein [Bacillus subtilis]
UniRef100_O32169

Bacillus subtilis

YusC


3301
Hypothetical protein yusD [Bacillus
UniRef100_O32170

Bacillus subtilis

YusD




subtilis]



3302
YusE protein [Bacillus subtilis]
UniRef100_O32171

Bacillus subtilis



3303
YusF protein [Bacillus subtilis]
UniRef100_O32172

Bacillus subtilis

YusF


3304


3305
Glycine cleavage system H protein
UniRef100_O32174

Bacillus subtilis

GcvH



[Bacillus subtilis]


3306
Hypothetical protein yusI [Bacillus
UniRef100_O32175

Bacillus subtilis

YusI




subtilis]



3307
YusJ protein [Bacillus subtilis]
UniRef100_O32176

Bacillus subtilis

YusJ


3308
YusK protein [Bacillus subtilis]
UniRef100_O32177

Bacillus subtilis

YusK


3309
YusL protein [Bacillus subtilis]
UniRef100_O32178

Bacillus subtilis

YusL


3310


3311


3312
YusN protein [Bacillus subtilis]
UniRef100_O32180

Bacillus subtilis

YusN


3313
Hypothetical protein yusU [Bacillus
UniRef100_O32187

Bacillus subtilis





subtilis]



3314
BH1040 protein [Bacillus halodurans]
UniRef100_Q9KE18

Bacillus halodurans



3315
YusV protein [Bacillus subtilis]
UniRef100_O32188

Bacillus subtilis

YusV


3316



YfhA


3317
YfiZ protein [Bacillus subtilis]
UniRef100_O31568

Bacillus subtilis

YfiZ


3318
YfiY protein [Bacillus subtilis]
UniRef100_O31567

Bacillus subtilis

YfiY


3319
Hypothetical protein yusW precursor
UniRef100_O32189

Bacillus subtilis

YusW



[Bacillus subtilis]


3320
YusX protein [Bacillus subtilis]
UniRef100_O32190

Bacillus subtilis

YusX


3321
D-alanyl-D-alanine carboxypeptidase
UniRef100_Q8ERG0

Oceanobacillus

DacB



[Oceanobacillus iheyensis]


iheyensis



3322
Hypothetical oxidoreductase yusZ
UniRef100_P37959

Bacillus subtilis

YusZ



[Bacillus subtilis]


3323
Metalloregulation DNA-binding stress
UniRef100_P37960

Bacillus subtilis

MrgA



protein [Bacillus subtilis]


3324
Probable serine protease yvtA [Bacillus
UniRef100_Q9R9I1

Bacillus subtilis

YvtA




subtilis]



3325
Transcriptional regulatory protein cssR
UniRef100_O32192

Bacillus subtilis

CssR



[Bacillus subtilis]


3326
Sensor protein cssS [Bacillus subtilis]
UniRef100_O32193

Bacillus subtilis

CssS


3327
YirB [Bacillus subtilis]
UniRef100_O32302

Bacillus subtilis



3328
Putative HTH-type transcriptional
UniRef100_P40950

Bacillus subtilis

YuxN



regulator yuxN [Bacillus subtilis]


3329
Fumarate hydratase class II [Bacillus
UniRef100_P07343

Bacillus subtilis

CitG




subtilis]



3330


3331
Spore germination protein A1 [Bacillus
UniRef100_P07868

Bacillus subtilis

GerAA




subtilis]



3332
Spore germination protein A2 [Bacillus
UniRef100_P07869

Bacillus subtilis

GerAB




subtilis]



3333
Spore germination protein A3 precursor
UniRef100_P07870

Bacillus subtilis

GerAC



[Bacillus subtilis]


3334


3335



YvqC


3336
YvqE protein [Bacillus subtilis]
UniRef100_O32198

Bacillus subtilis

YvqE


3337
YvqF protein [Bacillus subtilis]
UniRef100_O32199

Bacillus subtilis

YvqF


3338
YvqG protein [Bacillus subtilis]
UniRef100_O32200

Bacillus subtilis

YvqG


3339
Hypothetical protein yvqH [Bacillus
UniRef100_O32201

Bacillus subtilis

YvqH




subtilis]



3340
Hypothetical protein yvqI [Bacillus
UniRef100_O32202

Bacillus subtilis

YvqI




subtilis]



3341
Pectate lyase P358 [Bacillus sp. P-358]
UniRef100_Q8RR73

Bacillus sp. P-358



3342
YvqK protein [Bacillus subtilis]
UniRef100_O34899

Bacillus subtilis

YvqK


3343
UPI00002E3648 UniRef100 entry
UniRef100_UPI00002E3648

FabG


3344


3345



GbsB


3346



DapA


3347
Putative metal binding protein, YvrA
UniRef100_O34631

Bacillus subtilis

YvrA



[Bacillus subtilis]


3348
Putative hemin permease, YvrB
UniRef100_O34451

Bacillus subtilis

YvrB



[Bacillus subtilis]


3349
Putative metal binding protein, YvrC
UniRef100_O34805

Bacillus subtilis

YvrC



[Bacillus subtilis]


3350
Transcriptional regulator, GntR family
UniRef100_Q81SA7

Bacillus

YdhC



[Bacillus anthracis]


anthracis



3351
Putative ketoreductase, YvrD [Bacillus
UniRef100_O34782

Bacillus subtilis

YvrD




subtilis]



3352
UPI00003CC410 UniRef100 entry
UniRef100_UPI00003CC410

YflK


3353
Transcriptional regulators, LysR family
UniRef100_Q81DJ6

Bacillus cereus

AlsR



[Bacillus cereus]


3354
Exo-poly-alpha-D-galacturonosidase,
UniRef100_Q9WYR8

Thermotoga maritima




putative [Thermotoga maritima]


3355
Altronate hydrolase [Bacillus subtilis]
UniRef100_O34673

Bacillus subtilis

UxaA


3356
Altronate oxidoreductase [Bacillus
UniRef100_O34354

Bacillus subtilis

UxaB




subtilis]



3357
LacI repressor-like protein [Bacillus
UniRef100_Q9JMQ1

Bacillus subtilis

ExuR




subtilis]



3358
Hypothetical symporter yjmB [Bacillus
UniRef100_O34961

Bacillus subtilis

YjmB




subtilis]



3359
Uronate isomerase [Bacillus subtilis]
UniRef100_O34808

Bacillus subtilis

UxaC


3360
Putative sensory protein kinase, YvrG
UniRef100_O34989

Bacillus subtilis

YvrG



[Bacillus subtilis]


3361
Putative DNA binding response
UniRef100_P94504

Bacillus subtilis

YvrH



regulator, YvrH [Bacillus subtilis]


3362
Ferrichrome transport ATP-binding
UniRef100_P49938

Bacillus subtilis

FhuC



protein fhuC [Bacillus subtilis]


3363
Ferrichrome transport system
UniRef100_P49937

Bacillus subtilis

FhuG



permease protein fhuG [Bacillus




subtilis]



3364
Ferrichrome transport system
UniRef100_P49936

Bacillus subtilis

FhuB



permease protein fhuB [Bacillus




subtilis]



3365
Putative arginine ornithine antiporter,
UniRef100_O32204

Bacillus subtilis

YvsH



YvsH [Bacillus subtilis]


3366
Hypothetical protein yvsG precursor
UniRef100_O32205

Bacillus subtilis

YvsG



[Bacillus subtilis]


3367
Putative molybdate binding protein,
UniRef100_O32206

Bacillus subtilis

YvgJ



YvgJ [Bacillus subtilis]


3368



YcnB


3369
Putative reductase protein, YvgN
UniRef100_O32210

Bacillus subtilis

YvgN



[Bacillus subtilis]


3370
Fructokinase [Listeria monocytogenes]
UniRef100_Q722A5

Listeria

YdhR






monocytogenes



3371
Hypothetical protein ycbU precursor
UniRef100_P42253

Bacillus subtilis

YcbU



[Bacillus subtilis]


3372
Hypothetical protein CPE0889
UniRef100_Q8XM01

Clostridium perfringens




[Clostridium perfringens]


3373
YvgS protein [Bacillus subtilis]
UniRef100_O32215

Bacillus subtilis

YvgS


3374
Hypothetical UPF0126 protein yvgT
UniRef100_O32216

Bacillus subtilis

YvgT



[Bacillus subtilis]


3375
Glutamate-rich protein grpB [Bacillus
UniRef100_Q81CT5

Bacillus cereus

YqkA




cereus]



3376
Acetyltransferase, GNAT family
UniRef100_Q6HJN8

Bacillus

YuaI



[Bacillus thuringiensis]


thuringiensis



3377
Disulfide bond formation protein C
UniRef100_O32217

Bacillus subtilis

BdbC



[Bacillus subtilis]


3378
Disulfide bond formation protein D
UniRef100_O32218

Bacillus subtilis

BdbD



precursor [Bacillus subtilis]


3379
YvgW protein [Bacillus subtilis]
UniRef100_O32219

Bacillus subtilis

YvgW


3380
ABC transporter, substrate binding
UniRef100_Q8U7T8

Agrobacterium

YvfK



protein [Agrobacterium tumefaciens]


tumefaciens



3381
Putative sugar transport integral
UniRef100_Q9K442

Streptomyces

YurN



membrane protein [Streptomyces


coelicolor





coelicolor]



3382
Putative sugar transport integral
UniRef100_Q9K441

Streptomyces

YurM



membrane protein [Streptomyces


coelicolor





coelicolor]



3383
Alpha-glucosidase [Clostridium
UniRef100_Q97K36

Clostridium acetobutylicum





acetobutylicum]



3384
Glucan-glucohydrolase [Microbispora
UniRef100_Q59506

Microbispora

YbbD




bispora]



bispora



3385
ABC transporter, ATP-binding protein
UniRef100_Q81NK1

Bacillus

MntB



[Bacillus anthracis]


anthracis



3386
ABC transporter, permease protein
UniRef100_Q81NK2

Bacillus

MntD



[Bacillus anthracis]


anthracis



3387
ABC transporter, manganese-binding
UniRef100_Q71YG9

Listeria

YcdH



protein [Listeria monocytogenes]


monocytogenes



3388
Hypothetical protein [Bacillus cereus]
UniRef100_Q74NQ1

Bacillus cereus

YhjQ


3389
Phosphosugar-binding transcriptional
UniRef100_Q837Y3

Enterococcus

YfiA



regulator, RpiR family, putative


faecalis




[Enterococcus faecalis]


3390
1-phosphofructokinase [Enterococcus
UniRef100_Q837Y2

Enterococcus

FruK




faecalis]



faecalis



3391
PTS system, fructose-specific family,
UniRef100_Q837Y1

Enterococcus

FruA



IIBC components [Enterococcus


faecalis





faecalis]



3392
PTS system, IIA component
UniRef100_Q837Y0

Enterococcus

FruA



[Enterococcus faecalis]


faecalis



3393
Fructose/tagatose bisphosphate
UniRef100_Q7MC78

Vibrio vulnificus

FbaA



aldolase [Vibrio vulnificus]


3394


3395
Heavy metal-transporting ATPase
UniRef100_Q6HF81

Bacillus

YvgX



[Bacillus thuringiensis]


thuringiensis



3396
Copper chaperone copZ [Bacillus
UniRef100_O32221

Bacillus subtilis





subtilis]



3397
YvgZ protein [Bacillus subtilis]
UniRef100_O32222

Bacillus subtilis

YvgZ


3398
Intracellular proteinase [Thermoplasma
UniRef100_Q97BN0

Thermoplasma

YraA




volcanium]



volcanium



3399
Putative HTH-type transcriptional
UniRef100_O05261

Bacillus subtilis

YulB



regulator yulB [Bacillus subtilis]


3400
Rhamnulose kinase/L-fuculose kinase
UniRef100_Q8A1A3

Bacteroides

YulC



[Bacteroides thetaiotaomicron]


thetaiotaomicron



3401
Putative sugar isomerase
UniRef100_Q9XAB3

Streptomyces coelicolor




[Streptomyces coelicolor]


3402
Hypothetical protein [Polyangium
UniRef100_Q9L8B7

Polyangium cellulosum





cellulosum]



3403
Hypothetical oxidoreductase yuxG
UniRef100_P40747

Bacillus subtilis

YuxG



[Bacillus subtilis]


3404
YfhI protein [Bacillus subtilis]
UniRef100_O31577

Bacillus subtilis

YfhI


3405
YvmA [Bacillus subtilis]
UniRef100_O34307

Bacillus subtilis

YvmA


3406
YvmC [Bacillus subtilis]
UniRef100_O34351

Bacillus subtilis

YvmC


3407
Putative cytochrome P450 CYPX
UniRef100_O34926

Bacillus subtilis

CypX



[Bacillus subtilis]


3408
Hypothetical protein [Chromobacterium
UniRef100_Q7NRR6

Chromobacterium violaceum





violaceum]



3409



YvnA


3410
Hypothetical protein BRA0099 [Brucella
UniRef100_Q8FXH8

Brucella suis





suis]



3411
Chloroplast Toc64-2 [Physcomitrella
UniRef100_Q6RJN6

Physcomitrella

GatA




patens]



patens



3412
Hypothetical oxidoreductase yvaA
UniRef100_O32223

Bacillus subtilis

YvaA



[Bacillus subtilis]


3413
Putative acyl carrier protein
UniRef100_O32224

Bacillus subtilis

YvaB



phosphodiesterase 2 [Bacillus subtilis]


3414
Hypothetical UPF0176 protein ybfQ
UniRef100_O31457

Bacillus subtilis

YbfQ



[Bacillus subtilis]


3415
Putative DNA binding response
UniRef100_P94504

Bacillus subtilis




regulator, YvrH [Bacillus subtilis]


3416
YvrI protein [Bacillus subtilis]
UniRef100_O34843

Bacillus subtilis

YvrI


3417


3418
Oxalate decarboxylase oxdC [Bacillus
UniRef100_O34714

Bacillus subtilis

OxdC




subtilis]



3419
Hypothetical protein yvrL [Bacillus
UniRef100_O34686

Bacillus subtilis

YvrL




subtilis]



3420
BH3120 protein [Bacillus halodurans]
UniRef100_Q9K886

Bacillus halodurans



3421


3422
Group-specific protein [Bacillus cereus
UniRef100_Q633V1

Bacillus cereus ZK




ZK]


3423


3424


3425


3426
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069


3427
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012





anthracis str. A2012]



3428
LexA repressor [Listeria
UniRef100_Q720B9

Listeria monocytogenes





monocytogenes]



3429
Hypothetical protein [Bacillus cereus
UniRef100_Q630E5

Bacillus cereus ZK




ZK]


3430
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81XX1

Bacillus anthracis



3431


3432
Hypothetical protein RSc1705
UniRef100_Q8XYQ3

Ralstonia solanacearum




[Ralstonia solanacearum]


3433



YxiD


3434
Hypothetical protein [Bacillus
UniRef100_Q6HH72

Bacillus thuringiensis





thuringiensis]



3435



BlyA


3436


3437


3438


3439
YomQ protein [Bacteriophage SPBc2]
UniRef100_O64053
Bacteriophage
YomQ





SPBc2


3440



XkdV


3441


3442


3443



YubE


3444
ORF38 [Bacteriophage phi-105]
UniRef100_Q9ZXE5
Bacteriophage phi-105


3445



YqbO


3446
Hypothetical protein MW1895
UniRef100_Q8NVQ7

Staphylococcus aureus




[Staphylococcus aureus]


3447


3448


3449
Prophage LambdaBa02, major tail
UniRef100_Q81W97

Bacillus anthracis




protein, putative [Bacillus anthracis]


3450


3451
ORF32 [Bacteriophage phi-105]
UniRef100_Q9ZXF1
Bacteriophage phi-105


3452


3453
Gp7 protein [Bacteriophage phi3626]
UniRef100_Q8SBP7
Bacteriophage phi3626


3454
ORF29 [Bacteriophage phi-105]
UniRef100_Q9ZXF4
Bacteriophage phi-105


3455
ORF27 [Bacteriophage phi-105]
UniRef100_Q9ZXF6
Bacteriophage phi-105


3456
ClpP family serine protease, possible
UniRef100_Q97HW4

Clostridium

ClpP



phage related [Clostridium


acetobutylicum





acetobutylicum]



3457
ORF25 [Bacteriophage phi-105]
UniRef100_Q9ZXF8
Bacteriophage phi-105


3458


3459
Putative terminase large subunit
UniRef100_Q8SBQ2
Bacteriophage phi3626



[Bacteriophage phi3626]


3460
Phage terminase-like protein, small
UniRef100_Q97HW1

Clostridium acetobutylicum




subunit [Clostridium acetobutylicum]


3461
Prophage LambdaBa02, HNH
UniRef100_Q81W86

Bacillus anthracis




endonuclease family protein [Bacillus




anthracis]



3462
Spore coat protein D [Bacillus subtilis]
UniRef100_P07791

Bacillus subtilis



3463
ORF16 [Bacteriophage phi-105]
UniRef100_Q9ZXB9
Bacteriophage phi-105


3464


3465


3466
ORF14 [Bacteriophage phi-105]
UniRef100_Q9ZXC1
Bacteriophage phi-105


3467
ORF13 [Bacteriophage phi-105]
UniRef100_Q9ZXC2
Bacteriophage phi-105


3468
ORF12 [Bacteriophage phi-105]
UniRef100_Q9ZXC3
Bacteriophage phi-105


3469


3470
ORF11 [Bacteriophage phi-105]
UniRef100_Q9ZXC4
Bacteriophage phi-105


3471
ORF10 [Bacteriophage phi-105]
UniRef100_Q9ZXC5
Bacteriophage phi-105


3472
DNA, complete sequence
UniRef100_Q9ZXC7
Bacteriophage phi-105



[Bacteriophage phi-105]


3473
DNA, complete sequence
UniRef100_Q9ZXC8
Bacteriophage phi-105



[Bacteriophage phi-105]


3474


3475


3476


3477
Orf 36 [Staphylococcus aureus
UniRef100_Q9T1Z5

Staphylococcus aureus




bacteriophage PVL]

bacteriophage PVL


3478


3479
Orf16 [Bacteriophage bIL312]
UniRef100_Q9AZE4
Bacteriophage bIL312


3480
Gp26 protein [Bacteriophage phi3626]
UniRef100_Q8SBM8
Bacteriophage phi3626


3481
Cl like protein [Lactobacillus casei
UniRef100_O64370

Lactobacillus casei




bacteriophage A2]

bacteriophage A2


3482
Int gene product [Staphylococcus
UniRef100_Q932E5

Staphylococcus

YdcL




aureus]



aureus



3483
SsrA-binding protein [Bacillus subtilis]
UniRef100_O32230

Bacillus subtilis

SmpB


3484
Ribonuclease R [Bacillus subtilis]
UniRef100_O32231

Bacillus subtilis

Rnr


3485
YvaK protein [Bacillus subtilis]
UniRef100_O32232

Bacillus subtilis

YvaK


3486


3487


3488


3489



YvaO


3490



YvaO


3491
Glycine betaine/carnitine/choline
UniRef100_O34742

Bacillus subtilis

OpuCD



transport system permease protein



opuCD [Bacillus subtilis]


3492
Glycine betaine/carnitine/choline-
UniRef100_O32243

Bacillus subtilis

OpuCC



binding protein precursor [Bacillus




subtilis]



3493
Glycine betaine/carnitine/choline
UniRef100_O34878

Bacillus subtilis

OpuCB



transport system permease protein



opuCB [Bacillus subtilis]


3494
Glycine betaine/carnitine/choline
UniRef100_O34992

Bacillus subtilis

OpuCA



transport ATP-binding protein opuCA



[Bacillus subtilis]


3495
ORF-1 [Bacillus subtilis]
UniRef100_O34709

Bacillus subtilis

YvaV


3496



YvbG


3497



YvbI


3498
YvbJ protein [Bacillus subtilis]
UniRef100_O32247

Bacillus subtilis

YvbJ


3499
UPI00003CBDCC UniRef100 entry
UniRef100_UPI00003CBDCC

YfiY


3500


3501
YoaZ [Bacillus subtilis]
UniRef100_O34947

Bacillus subtilis

YoaZ


3502
Enolase [Bacillus subtilis]
UniRef100_P37869

Bacillus subtilis

Eno


3503
2,3-bisphosphoglycerate-independent
UniRef100_P39773

Bacillus subtilis

Pgm



phosphoglycerate mutase [Bacillus




subtilis]



3504
Triosephosphate isomerase [Bacillus
UniRef100_P27876

Bacillus subtilis

TpiA




subtilis]



3505
Phosphoglycerate kinase [Bacillus
UniRef100_P40924

Bacillus subtilis

Pgk




subtilis]



3506
Glyceraldehyde-3-phosphate
UniRef100_P09124

Bacillus subtilis

GapA



dehydrogenase 1 [Bacillus subtilis]


3507
Central glycolytic genes regulator
UniRef100_O32253

Bacillus subtilis

CggR



[Bacillus subtilis]


3508
Hypothetical protein yvbT [Bacillus
UniRef100_O32254

Bacillus subtilis

YvbT




subtilis]



3509
YvbW protein [Bacillus subtilis]
UniRef100_O32257

Bacillus subtilis

YvbW


3510


3511
ABC-type multidrug transport system,
UniRef100_Q8R7K3

Thermoanaerobacter

YfiL



ATPase component


tengcongensis




[Thermoanaerobacter tengcongensis]


3512


3513
YvbY protein [Bacillus subtilis]
UniRef100_O32259

Bacillus subtilis

YvbY


3514
YvfW protein [Bacillus subtilis]
UniRef100_O07021

Bacillus subtilis

YvfW


3515



YvfV


3516
Hypothetical protein yvfI [Bacillus
UniRef100_O07007

Bacillus subtilis

YvfI




subtilis]



3517
Putative L-lactate permease yvfH
UniRef100_P71067

Bacillus subtilis

YvfH



[Bacillus subtilis]


3518
RNA polymerase sigma-54 factor
UniRef100_P24219

Bacillus subtilis

SigL



[Bacillus subtilis]


3519
Hypothetical protein yvfG [Bacillus
UniRef100_P71066

Bacillus subtilis





subtilis]



3520
Hypothetical protein yvfF [Bacillus
UniRef100_P71065

Bacillus subtilis

YvfF




subtilis]



3521
YvfE protein [Bacillus subtilis]
UniRef100_Q795J3

Bacillus subtilis

YvfE


3522
Hypothetical protein yvfD [Bacillus
UniRef100_P71063

Bacillus subtilis

YvfD




subtilis]



3523
Hypothetical protein yvfC [Bacillus
UniRef100_P71062

Bacillus subtilis

YvfC




subtilis]



3524



YvfB


3525



YvfA


3526
Hypothetical protein yveT [Bacillus
UniRef100_P71059

Bacillus subtilis

YveT




subtilis]



3527
Hypothetical protein yveS [Bacillus
UniRef100_P71058

Bacillus subtilis

YveS




subtilis]



3528
Hypothetical protein yveR [Bacillus
UniRef100_P71057

Bacillus subtilis

YveR




subtilis]



3529
Hypothetical protein yveQ [Bacillus
UniRef100_P71056

Bacillus subtilis

YveQ




subtilis]



3530
Hypothetical protein yveP [Bacillus
UniRef100_P71055

Bacillus subtilis

YveP




subtilis]



3531
Hypothetical protein yveO [Bacillus
UniRef100_P71054

Bacillus subtilis

YveO




subtilis]



3532
Hypothetical protein yveN [Bacillus
UniRef100_P71053

Bacillus subtilis

YveN




subtilis]



3533
Hypothetical protein yveM [Bacillus
UniRef100_P71052

Bacillus subtilis

YveM




subtilis]



3534



YveL


3535
Chain length determinant protein
UniRef100_Q72XH7

Bacillus cereus

YveK



[Bacillus cereus]


3536
Hypothetical protein yveJ [Bacillus
UniRef100_P71049

Bacillus subtilis

Slr




subtilis]



3537
General stress protein 14 [Bacillus
UniRef100_P80871

Bacillus subtilis

YwrO




subtilis]



3538



YcbE


3539
UPI000027D43B UniRef100 entry
UniRef100_UPI000027D43B

YvcT


3540
4-hydroxythreonine-4-phosphate
UniRef100_Q8CUU4

Oceanobacillus iheyensis




dehydrogenase (EC 1.1.1.262) (4-



(phosphohydroxy)-L-threonine



dehydrogenase) [Oceanobacillus




iheyensis]



3541
Hypothetical conserved protein
UniRef100_Q8CUU5

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


3542



GbsB


3543



DapA


3544
Transcriptional regulator
UniRef100_Q8CUU7

Oceanobacillus

RocR



[Oceanobacillus iheyensis]


iheyensis



3545
Hypothetical protein [Bdellovibrio
UniRef100_Q6MH73

Bdellovibrio bacteriovorus





bacteriovorus]



3546
NADH-dependent flavin oxidoreductase
UniRef100_Q8ELB6

Oceanobacillus

YqiG



[Oceanobacillus iheyensis]


iheyensis



3547
Levansucrase precursor [Bacillus
UniRef100_P05655

Bacillus subtilis

SacB




subtilis]



3548
Hypothetical protein yveB [Bacillus
UniRef100_O07003

Bacillus subtilis

YveB




subtilis]



3549
Hypothetical protein [Pseudomonas
UniRef100_Q9I5B4

Pseudomonas aeruginosa





aeruginosa]



3550
ATP-dependent Clp protease
UniRef100_P80244

Bacillus subtilis

ClpP



proteolytic subunit [Bacillus subtilis]


3551
Hypothetical protein yvdD [Bacillus
UniRef100_O06986

Bacillus subtilis

YvdD




subtilis]



3552


3553
Possible metal-dependent
UniRef100_Q6HM73

Bacillus

YdhJ



phosphohydrolase; possible oxetanocin


thuringiensis




A resistance protein [Bacillus




thuringiensis]



3554
Hypothetical protein yvdB [Bacillus
UniRef100_O06984

Bacillus subtilis

YvdB




subtilis]



3555
Hypothetical protein yvdA [Bacillus
UniRef100_O06983

Bacillus subtilis

YvdA




subtilis]



3556
Probable 2-ketogluconate reductase
UniRef100_O32264

Bacillus subtilis

YvcT



[Bacillus subtilis]


3557
BH0392 protein [Bacillus halodurans]
UniRef100_Q9KFT5

Bacillus

YdfE






halodurans



3558
BH0391 protein [Bacillus halodurans]
UniRef100_Q9KFT6

Bacillus

YdfF






halodurans



3559
Ribonuclease [Paenibacillus polymyxa]
UniRef100_Q7MOX3

Paenibacillus polymyxa



3560
Barstar [Bacillus amyloliquefaciens]
UniRef100_P11540

Bacillus amyloliquefaciens



3561
Hypothetical protein yvcN [Bacillus
UniRef100_O06977

Bacillus subtilis

YvcN




subtilis]



3562
HPr-like protein crh [Bacillus subtilis]
UniRef100_O06976

Bacillus subtilis



3563
Hypothetical protein yvcL [Bacillus
UniRef100_O06975

Bacillus subtilis

YvcL




subtilis]



3564
Hypothetical UPF0052 protein yvcK
UniRef100_O06974

Bacillus subtilis

YvcK



[Bacillus subtilis]


3565
Hypothetical UPF0042 protein yvcJ
UniRef100_O06973

Bacillus subtilis

YvcJ



[Bacillus subtilis]


3566
Hypothetical protein yvcI [Bacillus
UniRef100_O06972

Bacillus subtilis

YvcI




subtilis]



3567
Thioredoxin reductase [Bacillus subtilis]
UniRef100_P80880

Bacillus subtilis

TrxB


3568
Hypothetical protein yvcD [Bacillus
UniRef100_O06968

Bacillus subtilis

YvcD




subtilis]



3569
Hypothetical conserved protein
UniRef100_Q8ETL1

Oceanobacillus

YbbJ



[Oceanobacillus iheyensis]


iheyensis



3570
Histidine biosynthesis bifunctional
UniRef100_O34912
Includes;
HisI



protein hisIE [Includes; Phosphoribosyl-

Phosphoribosyl-



AMP cyclohydrolase (EC 3.5.4.19)

AMP



(PRA-CH); Phosphoribosyl-ATP

cyclohydrolase



pyrophosphatase (EC 3.6.1.31) (PRA-

(EC 3.5.4.19)



PH)] [Bacillus subtilis]

(PRA-CH);





Phosphoribosyl-





ATP





pyrophosphatase





(EC 3.6.1.31)





(PRA-PH)


3571
Imidazole glycerol phosphate synthase
UniRef100_O34727

Bacillus subtilis

HisF



subunit hisF [Bacillus subtilis]


3572
1-(5-phosphoribosyl)-5-[(5-
UniRef100_O35006
(5-
HisA



phosphoribosylamino)methylideneamino]

phosphoribosylamino)



imidazole-4-carboxamide isomerase

methylideneamino



[Bacillus subtilis]


3573
Imidazole glycerol phosphate synthase
UniRef100_O34565

Bacillus subtilis

HisH



subunit hisH [Bacillus subtilis]


3574
Imidazoleglycerol-phosphate
UniRef100_O34683

Bacillus subtilis

HisB



dehydratase [Bacillus subtilis]


3575
Histidinol dehydrogenase [Bacillus
UniRef100_O34651

Bacillus subtilis

HisD




subtilis]



3576
ATP phosphoribosyltransferase
UniRef100_O34520

Bacillus subtilis

HisG



[Bacillus subtilis]


3577



HisZ


3578
YvpB [Bacillus subtilis]
UniRef100_O34735

Bacillus subtilis

YvpB


3579
Pectate lyase Pel-28K [Bacillus sp. P-
UniRef100_Q8L0R5

Bacillus sp. P-

YvpA



2850]

2850


3580
Putative acetyltransferase [Bacillus
UniRef100_O34993

Bacillus subtilis

YvoF




subtilis]



3581
Pyrophosphatase ppaX [Bacillus
UniRef100_Q9JMQ2

Bacillus subtilis

HprP




subtilis]



3582
YvoD [Bacillus subtilis]
UniRef100_O34382

Bacillus subtilis

YvoD


3583
Prolipoprotein diacylglyceryl transferase
UniRef100_O34752

Bacillus subtilis

Lgt



[Bacillus subtilis]


3584
HPr kinase/phosphorylase (EC 2.7.1.—)
UniRef100_O34483

Bacillus subtilis

HprK



(EC 2.7.4.—) (HPrK/P) (HPr(Ser)



kinase/phosphorylase) [Bacillus subtilis]


3585
YfiV protein [Bacillus subtilis]
UniRef100_O31564

Bacillus subtilis

YfiV


3586
YfiU protein [Bacillus subtilis]
UniRef100_O31563

Bacillus subtilis

YfiU


3587
YvnB [Bacillus subtilis]
UniRef100_O34986

Bacillus subtilis

YvnB


3588
YvlD [Bacillus subtilis]
UniRef100_O34648

Bacillus subtilis

YvlD


3589
YvlC [Bacillus subtilis]
UniRef100_O34719

Bacillus subtilis



3590
YvlB [Bacillus subtilis]
UniRef100_O34628

Bacillus subtilis

YvlB


3591
Hypothetical protein yvlA [Bacillus
UniRef100_O34322

Bacillus subtilis

YvlA




subtilis]



3592
Hypothetical protein yvkN [Bacillus
UniRef100_O34604

Bacillus subtilis





subtilis]



3593


3594
UvrABC system protein A [Bacillus
UniRef100_O34863

Bacillus subtilis

UvrA




subtilis]



3595
UvrABC system protein B [Bacillus
UniRef100_Q659H3

Bacillus

UvrB




amyloliquefaciens]



amyloliquefaciens



3596
CsbA protein [Bacillus subtilis]
UniRef100_P37953

Bacillus subtilis



3597


3598
YvjD [Bacillus subtilis]
UniRef100_O34375

Bacillus subtilis

YvjD


3599



YvzD


3600
Putative protease [Bacillus subtilis]
UniRef100_O35002

Bacillus subtilis

YvjB


3601
Tartrate dehydrogenase
UniRef100_Q8ESH5

Oceanobacillus

YcsA



[Oceanobacillus iheyensis]


iheyensis



3602
Cell wall-binding protein [Bacillus
UniRef100_Q9K6X4

Bacillus

YvcE




halodurans]



halodurans



3603
Cell division protein ftsX homolog
UniRef100_O34876

Bacillus subtilis

FtsX



[Bacillus subtilis]


3604
Cell division ATP-binding protein
UniRef100_O34814

Bacillus subtilis

FtsE



[Bacillus subtilis]


3605
Cytochrome c-551 [Bacillus subtilis]
UniRef100_O34594

Bacillus subtilis

CccB


3606
YvjA [Bacillus subtilis]
UniRef100_O34792

Bacillus subtilis

YvjA


3607
Peptide chain release factor 2 [Bacillus
UniRef100_P28367

Bacillus subtilis

PrfB




subtilis]



3608
Preprotein translocase secA subunit
UniRef100_P28366

Bacillus subtilis

SecA



[Bacillus subtilis]


3609
Hypothetical protein yvyD [Bacillus
UniRef100_P28368

Bacillus subtilis

YvyD




subtilis]



3610


3611
Flagellar protein fliT [Bacillus subtilis]
UniRef100_P39740

Bacillus subtilis

FliT


3612
Flagellar protein fliS [Bacillus subtilis]
UniRef100_P39739

Bacillus subtilis

FliS


3613
Flagellar hook-associated protein 2
UniRef100_P39738

Bacillus subtilis

FliD



[Bacillus subtilis]


3614
Hypothetical protein yvyC [Bacillus
UniRef100_P39737

Bacillus subtilis

YvyC




subtilis]



3615
Flagellin [Bacillus subtilis]
UniRef100_P02968

Bacillus subtilis

Hag


3616
Carbon storage regulator homolog
UniRef100_P33911

Bacillus subtilis




[Bacillus subtilis]


3617
Transmembrane protein [Bacillus
UniRef100_P96503

Bacillus subtilis

YviF




subtilis]



3618
YviE protein [Bacillus subtilis]
UniRef100_P96502

Bacillus subtilis

YviE


3619
Flagellar hook-associated protein 3
UniRef100_P96501

Bacillus subtilis

FlgL



[Bacillus subtilis]


3620
Flagellar hook-associated protein 1
UniRef100_P39810

Bacillus subtilis

FlgK



[Bacillus subtilis]


3621
Hypothetical protein yvyG [Bacillus
UniRef100_P39808

Bacillus subtilis

YvyG




subtilis]



3622
Negative regulator of flagellin synthesis
UniRef100_P39809

Bacillus subtilis




[Bacillus subtilis]


3623
Hypothetical protein yvyF [Bacillus
UniRef100_P39807

Bacillus subtilis

YvyF




subtilis]



3624



ComFC


3625


3626



ComFA


3627
DegV protein [Bacillus subtilis]
UniRef100_P32436

Bacillus subtilis

YviA


3628
Transcriptional regulatory protein degU
UniRef100_P13800

Bacillus subtilis

DegU



[Bacillus subtilis]


3629



DegS


3630
Hypothetical UPF0029 protein yvyE
UniRef100_P32437

Bacillus subtilis

YvyE



[Bacillus subtilis]


3631
Putative transcriptional regulator
UniRef100_P96499

Bacillus subtilis

YvhJ



[Bacillus subtilis]


3632
Hypothetical protein yunG [Bacillus
UniRef100_Q63CU2

Bacillus cereus

YunG




cereus ZK]


ZK


3633
Probable undecaprenyl-phosphate N-
UniRef100_O34753
Bacillus subtilis
TagO



acetylglucosaminyl 1-phosphate



transferase [Bacillus subtilis]


3634
Putative teichuronic acid biosynthesis
UniRef100_O32267

Bacillus subtilis

TuaH



glycosyl transferase tuaH [Bacillus




subtilis]



3635
Putative teichuronic acid biosynthesis
UniRef100_O32268

Bacillus subtilis

TuaG



glycosyl transferase tuaG [Bacillus




subtilis]



3636
Teichuronic acid biosynthesis protein
UniRef100_O32269

Bacillus subtilis

TuaF



tuaF [Bacillus subtilis]


3637
Teichuronic acid biosynthesis protein
UniRef100_O32270

Bacillus subtilis

TuaE



tuaE [Bacillus subtilis]


3638
Putative UDP-glucose 4-epimerase
UniRef100_Q57664

Methanococcus

GalE



[Methanococcus jannaschii]


jannaschii



3639
UDP-glucose 6-dehydrogenase
UniRef100_O32271

Bacillus subtilis

TuaD



[Bacillus subtilis]


3640
Putative teichuronic acid biosynthesis
UniRef100_O32272

Bacillus subtilis

TuaC



glycosyl transferase tuaC [Bacillus




subtilis]



3641
Teichuronic acid biosynthesis protein
UniRef100_O32273

Bacillus subtilis

TuaB



tuaB [Bacillus subtilis]


3642
UPI00003CB85E UniRef100 entry
UniRef100_UPI00003CB85E

TuaA


3643



LytC


3644
Amidase enhancer precursor [Bacillus
UniRef100_Q02113

Bacillus subtilis

LytB




subtilis]



3645



LytA


3646
Membrane-bound protein lytR [Bacillus
UniRef100_Q02115

Bacillus subtilis

LytR




subtilis]



3647
UDP-N-acetylglucosamine 2-epimerase
UniRef100_P39131

Bacillus subtilis

YvyH



[Bacillus subtilis]


3648
UTP--glucose-1-phosphate
UniRef100_Q05852

Bacillus subtilis

GtaB



uridylyltransferase [Bacillus subtilis]


3649



TagH


3650
Teichoic acid translocation permease
UniRef100_P42953

Bacillus subtilis

TagG



protein tagG [Bacillus subtilis]


3651
Teichoic acid biosynthesis protein
UniRef100_Q9CH20

Lactococcus

TagF



[Lactococcus lactis]


lactis



3652
CDP-glycerol: poly(glycerophosphate)
UniRef100_P13485

Bacillus subtilis

TagF



glycerophosphotransferase [Bacillus




subtilis]



3653



TagD


3654



TagA


3655
Putative CDP-
UniRef100_P27621

Bacillus subtilis

TagB



glycerol:glycerophosphate



glycerophosphotransferase [Bacillus




subtilis]



3656
Beta-N-acetylglucosaminidase
UniRef100_P39848

Bacillus subtilis

LytD



precursor [Bacillus subtilis]


3657
Mannose-6-phosphate isomerase
UniRef100_P39841

Bacillus subtilis

Pmi



[Bacillus subtilis]


3658


3659
Anaerobic ribonucleoside-triphosphate
UniRef100_Q733N6

Bacillus cereus




reductase, putative [Bacillus cereus]


3660
2-keto-3-deoxygluconate permease
UniRef100_P50847

Bacillus subtilis

KdgT



[Bacillus subtilis]


3661
KHG/KDPG aldolase [Includes: 4-
UniRef100_P50846
Includes: 4-
KdgA



hydroxy-2-oxoglutarate aldolase (EC

hydroxy-2-



4.1.3.16) (2-keto-4-hydroxyglutarate

oxoglutarate



aldolase) (KHG-aldolase); 2-dehydro-

aldolase (EC



3-deoxy-phosphogluconate aldolase

4.1.3.16) (2-keto-



(EC 4.1.2.14) (Phospho-2-dehydro-3-

4-



deoxygluconate aldolase) (Phospho-2-

hydroxyglutarate



keto-3-d

aldolase) (KHG-





aldolase); 2-





dehydro-3-





deoxy-





phosphogluconate





aldolase (EC





4.1.2.14)





(Phospho-2-





dehydro-3-





deoxygluconate





aldolase)





(Phospho-2-keto-





3-





deoxygluconate





aldol


3662
2-dehydro-3-deoxygluconokinase
UniRef100_P50845

Bacillus subtilis

KdgK



[Bacillus subtilis]


3663
HTH-type transcriptional regulator kdgR
UniRef100_P50844

Bacillus subtilis

KdgR



[Bacillus subtilis]


3664



KduI


3665
2-deoxy-D-gluconate 3-dehydrogenase
UniRef100_P50842

Bacillus subtilis

KduD



[Bacillus subtilis]


3666
Alcohol dehydrogenase, glutathione-
UniRef100_Q6HGX1

Bacillus

AdhB



dependent formaldehyde


thuringiensis




dehydrogenase [Bacillus thuringiensis]


3667
YwtG protein [Bacillus subtilis]
UniRef100_P96742

Bacillus subtilis

YwtG


3668
Hypothetical transcriptional regulator
UniRef100_Q7WY78

Bacillus subtilis

YwtF



ywtF [Bacillus subtilis]


3669
YwtE protein [Bacillus subtilis]
UniRef100_P96741

Bacillus subtilis

YwtE


3670
Gamma-DL-glutamyl hydrolase
UniRef100_P96740

Bacillus subtilis

YwtD



precursor [Bacillus subtilis]


3671


3672
YwtB protein [Bacillus subtilis]
UniRef100_P96738

Bacillus subtilis

YwtB


3673
YwtA protein [Bacillus subtilis]
UniRef100_P96737

Bacillus subtilis

YwtA


3674
YwsC protein [Bacillus subtilis]
UniRef100_P96736

Bacillus subtilis

YwsC


3675



RbsR


3676
Ribokinase [Bacillus subtilis]
UniRef100_P36945

Bacillus subtilis

RbsK


3677
High affinity ribose transport protein
UniRef100_P36946

Bacillus subtilis

RbsD



rbsD [Bacillus subtilis]


3678
Ribose transport ATP-binding protein
UniRef100_P36947

Bacillus subtilis

RbsA



rbsA [Bacillus subtilis]


3679
Ribose transport system permease
UniRef100_P36948

Bacillus subtilis

RbsC



protein rbsC [Bacillus subtilis]


3680
D-ribose-binding protein precursor
UniRef100_P36949

Bacillus subtilis

RbsB



[Bacillus subtilis]


3681
Alpha-acetolactate decarboxylase
UniRef100_Q04777

Bacillus subtilis

AlsD



[Bacillus subtilis]


3682
Acetolactate synthase [Bacillus subtilis]
UniRef100_Q04789

Bacillus subtilis

AlsS


3683
HTH-type transcriptional regulator alsR
UniRef100_Q04778

Bacillus subtilis

AlsR



[Bacillus subtilis]


3684
YwrD protein [Bacillus subtilis]
UniRef100_O05218

Bacillus subtilis

YwrD


3685
YwrC protein [Bacillus subtilis]
UniRef100_O05217

Bacillus subtilis

YwrC


3686
YwrB protein [Bacillus subtilis]
UniRef100_O05216

Bacillus subtilis

YwrB


3687
YwrA protein [Bacillus subtilis]
UniRef100_O05215

Bacillus subtilis

YwrA


3688
Putative protein-tyrosine phosphatase
UniRef100_P96717

Bacillus subtilis

YwqE



ywqE [Bacillus subtilis]


3689
Hypothetical protein ywqC [Bacillus
UniRef100_P96715

Bacillus subtilis

YwqC




subtilis]



3690
Hypothetical protein ywqB [Bacillus
UniRef100_P96714

Bacillus subtilis

YwqB




subtilis]



3691
YwqA protein [Bacillus subtilis]
UniRef100_P94593

Bacillus subtilis

YwqA


3692
YwpJ protein [Bacillus subtilis]
UniRef100_P94592

Bacillus subtilis

YwpJ


3693
YwpI protein [Bacillus subtilis]
UniRef100_P94591

Bacillus subtilis

GlcR


3694
Fructose-6-phosphate aldolase 2
UniRef100_P58424

Escherichia coli

YwjH



[Escherichia coli O157:H7]

O157:H7


3695
Hypothetical protein PM1968
UniRef100_Q9CJM9

Pasteurella

FabG



[Pasteurella multocida]


multocida



3696
PTS system, sorbitol-specific IIA
UniRef100_Q82YX6

Enterococcus faecalis




component [Enterococcus faecalis]


3697
PTS system, sorbitol-specific IIBC
UniRef100_Q82YX5

Enterococcus faecalis




components [Enterococcus faecalis]


3698
UPI00002F4A3A UniRef100 entry
UniRef100_UPI00002F4A3A


3699
Sorbitol operon activator
UniRef100_Q9X673

Streptococcus mutans




[Streptococcus mutans]


3700
Putative transcriptional regulator of
UniRef100_Q83PA8

Shigella flexneri

DeoR



sorbose uptake and utilization genes



[Shigella flexneri]


3701
YwpH protein [Bacillus subtilis]
UniRef100_P94590

Bacillus subtilis

YwpH


3702
Hypothetical protein ywpG [Bacillus
UniRef100_P94589

Bacillus subtilis

YwpG




subtilis]



3703
YwpF protein [Bacillus subtilis]
UniRef100_P94588

Bacillus subtilis

YwpF


3704
Large-conductance mechanosensitive
UniRef100_P94585

Bacillus subtilis

MscL



channel [Bacillus subtilis]


3705
(3R)-hydroxymyristoyl-[acyl carrier
UniRef100_Q81JE0
acyl carrier
YwpB



protein] dehydratase (EC 4.2.1.—) ((3R)-

protein



hydroxymyristoyl ACP dehydrase)



[Bacillus anthracis]


3706
Flagellar hook-basal body complex
UniRef100_P39753

Bacillus subtilis

FlhP



protein flhP [Bacillus subtilis]


3707
Flagellar hook-basal body complex
UniRef100_P39752

Bacillus subtilis

FlhO



protein flhO [Bacillus subtilis]


3708
MreB-like protein [Bacillus subtilis]
UniRef100_P39751

Bacillus subtilis

MbI


3709
Stage III sporulation protein D [Bacillus
UniRef100_P15281

Bacillus subtilis





subtilis]



3710


3711
Hypothetical protein ywoH [Bacillus
UniRef100_P94578

Bacillus subtilis

YwoH




subtilis]



3712
Hypothetical protein ywoG [Bacillus
UniRef100_P94577

Bacillus subtilis

YwoG




subtilis]



3713
Hypothetical protein ywoA [Bacillus
UniRef100_P94571

Bacillus subtilis

YwoA




subtilis]



3714
Manganese-dependent inorganic
UniRef100_Q63AC7

Bacillus cereus

PpaC



pyrophosphatase [Bacillus cereus ZK]

ZK


3715
Probable manganese catalase [Bacillus
UniRef100_P80878

Bacillus subtilis

YdbD




subtilis]



3716
Hypothetical protein yqjF [Bacillus
UniRef100_P54543

Bacillus subtilis

YqjF




subtilis]



3717
BH2605 protein [Bacillus halodurans]
UniRef100_Q9K9P0

Bacillus

YhcV






halodurans



3718
Hypothetical protein yqxL [Bacillus
UniRef100_P40948

Bacillus subtilis

YqxL




subtilis]



3719
Hypothetical UPF0053 protein yqhB
UniRef100_P54505

Bacillus subtilis

YqhB



[Bacillus subtilis]


3720
Ammonium transporter nrgA [Bacillus
UniRef100_Q07429

Bacillus subtilis

NrgA




subtilis]



3721
Nitrogen regulatory PII-like protein
UniRef100_Q07428

Bacillus subtilis

NrgB



[Bacillus subtilis]


3722
ATP-dependent Clp protease
UniRef100_Q81CH1

Bacillus cereus

ClpP



proteolytic subunit [Bacillus cereus]


3723
RNA polymerase ECF-type sigma
UniRef100_Q63AF9

Bacillus cereus

SigM



factor [Bacillus cereus ZK]

ZK


3724
Hypothetical protein ywnI [Bacillus
UniRef100_P71044

Bacillus subtilis

SpoIIQ




subtilis]



3725
Hypothetical protein ywnH [Bacillus
UniRef100_P71043

Bacillus subtilis

YwnH




subtilis]



3726
Hypothetical protein ywnF [Bacillus
UniRef100_P71041

Bacillus subtilis

YwnF




subtilis]



3727
Probable cardiolipin synthetase 2
UniRef100_P71040

Bacillus subtilis

YwnE



[Bacillus subtilis]


3728
UPI00003CB764 UniRef100 entry
UniRef100_UPI00003CB764

YhcA


3729
Antibiotic/siderophore biosynthesis
UniRef100_Q81DP7

Bacillus cereus




protein [Bacillus cereus]


3730
Probable serine activating enzyme
UniRef100_P45745

Bacillus subtilis

DhbF



[Bacillus subtilis]


3731
Isochorismatase [Bacillus subtilis]
UniRef100_P45743

Bacillus subtilis

DhbB


3732
2,3-dihydroxybenzoate-AMP ligase
UniRef100_P40871

Bacillus subtilis

DhbE



[Bacillus subtilis]


3733
Isochorismate synthase dhbC [Bacillus
UniRef100_P45744

Bacillus subtilis

DhbC




subtilis]



3734
2,3-dihydro-2,3-dihydroxybenzoate
UniRef100_P39071

Bacillus subtilis

DhbA



dehydrogenase [Bacillus subtilis]


3735
YuiI protein [Bacillus subtilis]
UniRef100_O32102

Bacillus subtilis

YuiI


3736
Iron-uptake system permease protein
UniRef100_P40411

Bacillus subtilis

FeuC



feuC [Bacillus subtilis]


3737
Iron-uptake system permease protein
UniRef100_P40410

Bacillus subtilis

FeuB



feuB [Bacillus subtilis]


3738
Iron-uptake system binding protein
UniRef100_P40409

Bacillus subtilis

FeuA



precursor [Bacillus subtilis]


3739
Putative HTH-type transcriptional
UniRef100_P40408

Bacillus subtilis

YbbB



regulator ybbB [Bacillus subtilis]


3740
Hypothetical protein ywnG [Bacillus
UniRef100_P71042

Bacillus subtilis

YwnC




subtilis]



3741
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069


3742
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012





anthracis str. A2012]



3743
Hypothetical protein ywnB [Bacillus
UniRef100_P71037

Bacillus subtilis

YwnB




subtilis]



3744
Hypothetical protein ywnA [Bacillus
UniRef100_P71036

Bacillus subtilis

YwnA




subtilis]



3745


3746
Hypothetical protein ywmF [Bacillus
UniRef100_P70963

Bacillus subtilis

YwmF




subtilis]



3747



MoaA


3748
FdhD protein homolog [Bacillus subtilis]
UniRef100_P39756

Bacillus subtilis

FdhD


3749
BH2528 protein [Bacillus halodurans]
UniRef100_Q9K9W7

Bacillus

YbfB






halodurans



3750
Formate dehydrogenase alpha chain
UniRef100_Q9K9W5

Bacillus

YjgC



[Bacillus halodurans]


halodurans



3751



YwmD


3752
Hypothetical protein ywmC precursor
UniRef100_P70960

Bacillus subtilis

YwmC



[Bacillus subtilis]


3753
Stage II sporulation protein D [Bacillus
UniRef100_P07372

Bacillus subtilis

SpoIID




subtilis]



3754
UDP-N-acetylglucosamine 1-
UniRef100_P70965

Bacillus subtilis

MurAA



carboxyvinyltransferase 1 [Bacillus




subtilis]



3755
YwmB protein [Bacillus subtilis]
UniRef100_O32277

Bacillus subtilis

YwmB


3756
YwzB protein [Bacillus subtilis]
UniRef100_O32278

Bacillus subtilis



3757
ATP synthase epsilon chain [Bacillus
UniRef100_P37812

Bacillus subtilis

AtpC




subtilis]



3758
ATP synthase beta chain [Bacillus
UniRef100_P37809

Bacillus subtilis

AtpD




subtilis]



3759
ATP synthase gamma chain [Bacillus
UniRef100_P37810

Bacillus subtilis

AtpG




subtilis]



3760
ATP synthase alpha chain [Bacillus
UniRef100_Q659H2

Bacillus

AtpA




amyloliquefaciens]



amyloliquefaciens



3761
ATP synthase delta chain [Bacillus
UniRef100_P37811

Bacillus subtilis

AtpH




subtilis]



3762
ATP synthase B chain [Bacillus subtilis]
UniRef100_P37814

Bacillus subtilis

AtpF


3763
ATP synthase a chain [Bacillus subtilis]
UniRef100_P37813

Bacillus subtilis

AtpB


3764



AtpI


3765
Uracil phosphoribosyltransferase
UniRef100_P39149

Bacillus subtilis

Upp



[Bacillus subtilis]


3766
Serine hydroxymethyltransferase
UniRef100_P39148

Bacillus subtilis

GlyA



[Bacillus subtilis]


3767
Hypothetical protein ywlG [Bacillus
UniRef100_P39157

Bacillus subtilis

YwlG




subtilis]



3768
Hypothetical lacA/rpiB family protein
UniRef100_P39156

Bacillus subtilis

YwlF



ywlF [Bacillus subtilis]


3769
Putative low molecular weight protein-
UniRef100_P39155

Bacillus subtilis

YwlE



tyrosine-phosphatase ywlE [Bacillus




subtilis]



3770
Hypothetical UPF0059 protein ywlD
UniRef100_P39154

Bacillus subtilis

YwlD



[Bacillus subtilis]


3771
Hypothetical protein ywlC [Bacillus
UniRef100_P39153

Bacillus subtilis

YwlC




subtilis]



3772
Hypothetical protein ywlB [Bacillus
UniRef100_P39152

Bacillus subtilis

YwlB




subtilis]



3773
Stage II sporulation protein R [Bacillus
UniRef100_P39151

Bacillus subtilis

SpoIIR




subtilis]



3774
Hypothetical protein yhjI [Bacillus
UniRef100_O07563

Bacillus subtilis

GlcP




subtilis]



3775
Hypothetical protein yhjJ [Bacillus
UniRef100_O07564

Bacillus subtilis

YhjJ




subtilis]



3776
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81NC8

Bacillus

YhjK






anthracis



3777
Hypothetical protein yhjL [Bacillus
UniRef100_O07566

Bacillus subtilis

YhjL




subtilis]



3778
Hypothetical protein yhjM [Bacillus
UniRef100_O07567

Bacillus subtilis

YhjM




subtilis]



3779
HemK protein homolog [Bacillus
UniRef100_P45873

Bacillus subtilis

YwkE




subtilis]



3780
Peptide chain release factor 1 [Bacillus
UniRef100_P45872

Bacillus subtilis

PrfA




subtilis]



3781
Hypothetical protein ywkD [Bacillus
UniRef100_P45871

Bacillus subtilis

YwkD




subtilis]



3782
Hypothetical protein ywkC [Bacillus
UniRef100_P45870

Bacillus subtilis

YwkC




subtilis]



3783
Hypothetical protein ywkB [Bacillus
UniRef100_P45869

Bacillus subtilis

YwkB




subtilis]



3784
Probable NAD-dependent malic
UniRef100_P45868

Bacillus subtilis

YwkA



enzyme 2 [Bacillus subtilis]


3785
Thymidine kinase [Bacillus subtilis]
UniRef100_Q03221

Bacillus subtilis

Tdk


3786
50S ribosomal protein L31 [Bacillus
UniRef100_Q03223

Bacillus subtilis





subtilis]



3787
Transcription termination factor rho
UniRef100_Q03222

Bacillus subtilis

Rho



[Bacillus subtilis]


3788
Hypothetical protein ywjI [Bacillus
UniRef100_Q03224

Bacillus subtilis

YwjI




subtilis]



3789
UDP-N-acetylglucosamine 1-
UniRef100_P19670

Bacillus subtilis

MurAB



carboxyvinyltransferase 2 [Bacillus




subtilis]



3790
Transaldolase [Bacillus subtilis]
UniRef100_P19669

Bacillus subtilis

YwjH


3791
Probable fructose-bisphosphate
UniRef100_P13243

Bacillus subtilis

FbaA



aldolase 1 [Bacillus subtilis]


3792
Sporulation initiation
UniRef100_P06628

Bacillus subtilis

Spo0F



phosphotransferase F [Bacillus subtilis]


3793
Hypothetical protein ywjG [Bacillus
UniRef100_P06629

Bacillus subtilis

YwjG




subtilis]



3794



PyrG


3795
DNA-directed RNA polymerase delta
UniRef100_P12464

Bacillus subtilis

RpoE



subunit [Bacillus subtilis]


3796
Acyl-CoA dehydrogenase [Bacillus
UniRef100_Q814S9

Bacillus cereus

AcdA




cereus]



3797
Acyl-CoA dehydrogenase [Bacillus
UniRef100_Q81JV7

Bacillus

MmgC




anthracis]



anthracis



3798
UPI00003CC303 UniRef100 entry
UniRef100_UPI00003CC303

MmgB


3799
Acetyl-CoA acetyltransferase
UniRef100_Q8EM47

Oceanobacillus

MmgA



[Oceanobacillus iheyensis]


iheyensis



3800
Hypothetical protein ywjF [Bacillus
UniRef100_P45866

Bacillus subtilis

YwjF




subtilis]



3801



YwjE


3802
Hypothetical protein ywjC [Bacillus
UniRef100_P45863

Bacillus subtilis





subtilis]



3803
Arginyl-tRNA synthetase [Bacillus
UniRef100_P46906

Bacillus subtilis

ArgS




subtilis]



3804



YwiB


3805
Agmatinase [Bacillus subtilis]
UniRef100_P70999

Bacillus subtilis

SpeB


3806
Spermidine synthase [Bacillus subtilis]
UniRef100_P70998

Bacillus subtilis

SpeE


3807
UPI00002A1DD1 UniRef100 entry
UniRef100_UPI00002A1DD1


3808
Hypothetical protein ywhE [Bacillus
UniRef100_P70997

Bacillus subtilis

YwhE




subtilis]



3809
Hypothetical protein ywhD [Bacillus
UniRef100_P70996

Bacillus subtilis

YwhD




subtilis]



3810
Hypothetical protein ywhC [Bacillus
UniRef100_P70995

Bacillus subtilis

YwhC




subtilis]



3811
Probable tautomerase ywhB [Bacillus
UniRef100_P70994

Bacillus subtilis





subtilis]



3812
Conserved protein [Methanosarcina
UniRef100_Q8PUC6

Methanosarcina mazei





mazei]



3813
Hypothetical protein ywgA [Bacillus
UniRef100_P71046

Bacillus subtilis

YwgA




subtilis]



3814
Hypothetical protein ywfO [Bacillus
UniRef100_P39651

Bacillus subtilis

YwfO




subtilis]



3815
YwzC protein [Bacillus subtilis]
UniRef100_O32280

Bacillus subtilis



3816
Prespore specific transcriptional
UniRef100_P39650

Bacillus subtilis

RsfA



regulator rsfA [Bacillus subtilis]


3817
Hypothetical protein yjdJ precursor
UniRef100_O31651

Bacillus subtilis




[Bacillus subtilis]


3818
Hypothetical protein ywfL [Bacillus
UniRef100_P39648

Bacillus subtilis

YwfL




subtilis]



3819
4-hydroxybenzoate 3-monooxygenase
UniRef100_Q59724

Pseudomonas sp

YhjG



[Pseudomonas sp]


3820
Benzoate transporter protein
UniRef100_Q6XUN5

Pseudomonas

YceI



[Pseudomonas sp. ND6]

sp. ND6


3821
Putative 3,4-dihydroxyphenylacetate
UniRef100_Q6N986

Rhodopseudomonas palustris




2,3-dioxygenase [Rhodopseudomonas




palustris]



3822
4-oxalocrotonate tautomerase
UniRef100_Q9Z431

Pseudomonas putida




[Pseudomonas putida]


3823
4-oxalocrotonate decarboxylase
UniRef100_Q84I96

Pseudomonas sp. S-47




[Pseudomonas sp. S-47]


3824



DhaS


3825
Putative transcriptional regulator
UniRef100_Q9F7C7

Agrobacterium

KipR



[Agrobacterium tumefaciens]


tumefaciens



3826
Hypothetical protein SCO6305
UniRef100_Q93RT6

Streptomyces coelicolor




[Streptomyces coelicolor]


3827
Phosphate acetyltransferase [Bacillus
UniRef100_P39646

Bacillus subtilis

Pta




subtilis]



3828
Hypothetical protein ywfI [Bacillus
UniRef100_P39645

Bacillus subtilis

YwfI




subtilis]



3829



YuaB


3830
Na+-transporting ATP synthase
UniRef100_Q9KF87

Bacillus

YubG



[Bacillus halodurans]


halodurans



3831
Hypothetical protein ywdL [Bacillus
UniRef100_P39620

Bacillus subtilis

YwdL




subtilis]



3832
Multidrug ABC transporter, permease
UniRef100_Q63D13

Bacillus cereus

YfiN



[Bacillus cereus ZK]

ZK


3833
ABC transporter, permease protein
UniRef100_Q73AB4

Bacillus cereus

YfiM



[Bacillus cereus]


3834
Multidrug ABC transporter, ATP-binding
UniRef100_Q63D15

Bacillus cereus

YfiL



protein [Bacillus cereus ZK]

ZK


3835
Sensor histidine kinase, putative
UniRef100_Q73AB6

Bacillus cereus

YfiJ



[Bacillus cereus]


3836
DNA-binding response regulator, LuxR
UniRef100_Q73AB7

Bacillus cereus

YfiK



family [Bacillus cereus]


3837



YwdK


3838
Putative purine permease ywdJ
UniRef100_P39618

Bacillus subtilis

YwdJ



[Bacillus subtilis]


3839
Hypothetical protein ywdI [Bacillus
UniRef100_P39617

Bacillus subtilis

YwdI




subtilis]



3840
Probable aldehyde dehydrogenase
UniRef100_P39616

Bacillus subtilis

YwdH



ywdH [Bacillus subtilis]


3841
Uracil-DNA glycosylase [Bacillus
UniRef100_P39615

Bacillus subtilis

Ung




subtilis]



3842



YwdF


3843
Phosphomethylpyrimidine kinase
UniRef100_P39610

Bacillus subtilis

ThiD



[Bacillus subtilis]


3844
Hypothetical protein ywdA [Bacillus
UniRef100_P39609

Bacillus subtilis





subtilis]



3845
Sucrose-6-phosphate hydrolase
UniRef100_P07819

Bacillus subtilis

SacA



[Bacillus subtilis]


3846
PTS system, sucrose-specific IIBC
UniRef100_P05306

Bacillus subtilis

SacP



component [Bacillus subtilis]


3847



SacT


3848


3849
Minor extracellular protease vpr
UniRef100_P29141

Bacillus subtilis

Vpr



precursor [Bacillus subtilis]


3850


3851



YwcH


3852
Nitro/flavin reductase [Bacillus subtilis]
UniRef100_P39605

Bacillus subtilis

NfrA


3853
N-acyl-L-amino acid amidohydrolase
UniRef100_Q9KCF8

Bacillus

YhaA



[Bacillus halodurans]


halodurans



3854
Hypothetical protein ywcF [Bacillus
UniRef100_P39604

Bacillus subtilis

RodA




subtilis]



3855



RodA


3856
Membrane protein [Clostridium tetani]
UniRef100_Q897A1

Clostridium tetani



3857
Hypothetical protein ywcE precursor
UniRef100_P39603

Bacillus subtilis




[Bacillus subtilis]


3858
Clostripain [Methanosarcina
UniRef100_Q8TIY6

Methanosarcina acetivorans





acetivorans]



3859
YfmK protein [Bacillus subtilis]
UniRef100_O34536

Bacillus subtilis

YfmK


3860
ABC transporter, ATP-
UniRef100_Q882Y2

Pseudomonas

YgaD



binding/permease protein


syringae




[Pseudomonas syringae]


3861
HlyB/MsbA family ABC transporter
UniRef100_Q7NIB9

Gloeobacter

YwjA



[Gloeobacter violaceus]


violaceus



3862


3863


3864


3865


3866
Response regulator [Bacillus cereus
UniRef100_Q63FE4

Bacillus cereus

ComA



ZK]

ZK


3867


3868
Quinol oxidase polypeptide IV [Bacillus
UniRef100_P34959

Bacillus subtilis

QoxD




subtilis]



3869
Quinol oxidase polypeptide III (EC
UniRef100_P34958

Bacillus subtilis

QoxC



1.10.3.—) (Quinol oxidase aa3-600,



subunit qoxC) (Oxidase aa(3)-600



subunit 3) [Bacillus subtilis]


3870
Quinol oxidase polypeptide I (EC
UniRef100_P34956

Bacillus subtilis

QoxB



1.10.3.—) (Quinol oxidase aa3-600,



subunit qoxB) (Oxidase aa(3)-600



subunit 1) [Bacillus subtilis]


3871



QoxA


3872
UDP-glucose 4-epimerase
UniRef100_Q8R8R8

Thermoanaerobacter

GalE



[Thermoanaerobacter tengcongensis]


tengcongensis



3873


3874


3875


3876
Hypothetical protein ywqN [Bacillus
UniRef100_P96726

Bacillus subtilis

YwqN




subtilis]



3877
Hypothetical protein ywcB [Bacillus
UniRef100_P39600

Bacillus subtilis





subtilis]



3878
Putative symporter ywcA [Bacillus
UniRef100_P39599

Bacillus subtilis

YwcA




subtilis]



3879



XylB


3880
Xylose isomerase [Bacillus sp.]
UniRef100_P54272

Bacillus sp.

XylA


3881



XylR


3882
Thiamine-phosphate
UniRef100_P39594

Bacillus subtilis

ThiE



pyrophosphorylase [Bacillus subtilis]


3883
Hydroxyethylthiazole kinase [Bacillus
UniRef100_P39593

Bacillus subtilis

ThiM




subtilis]



3884
Putative HTH-type transcriptional
UniRef100_P39592

Bacillus subtilis

YwbI



regulator ywbI [Bacillus subtilis]


3885
Putative HTH-type transcriptional
UniRef100_P39592

Bacillus subtilis

YwbI



regulator ywbI [Bacillus subtilis]


3886


3887



YwqH


3888
Holin-like protein cidA [Bacillus subtilis]
UniRef100_P39591

Bacillus subtilis

YwbH


3889
Hypothetical protein ywbG [Bacillus
UniRef100_P39590

Bacillus subtilis

YwbG




subtilis]



3890
HlyC domain protein [Bacillus cereus]
UniRef100_Q731T5

Bacillus cereus



3891



OpuE


3892
Hypothetical UPF0064 protein ywbD
UniRef100_P39587

Bacillus subtilis

YwbD



[Bacillus subtilis]


3893
Hypothetical protein ywbC [Bacillus
UniRef100_P39586

Bacillus subtilis

YwbC




subtilis]



3894
Phosphoglycerate dehydrogenase
UniRef100_Q8GCC8

Bacillus

YvcT



[Bacillus coagulans]


coagulans



3895
Glycerate dehydrogenase
UniRef100_Q8EP33

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


3896
General stress protein A [Bacillus
UniRef100_P25148

Bacillus subtilis

GspA




subtilis]



3897
YdfH protein [Bacillus subtilis]
UniRef100_P96685

Bacillus subtilis

YdfH


3898
YdfI protein [Bacillus subtilis]
UniRef100_P96686

Bacillus subtilis

YdfI


3899
YdfJ protein [Bacillus subtilis]
UniRef100_P96687

Bacillus subtilis

YdfJ


3900
Hypothetical protein ywaF [Bacillus
UniRef100_P25149

Bacillus subtilis

YwaF




subtilis]



3901
MFS transporter, phthalate permease
UniRef100_Q884B5

Pseudomonas

YcbE



family [Pseudomonas syringae]


syringae



3902
Hypothetical protein OB2215
UniRef100_Q8EP98

Oceanobacillus

YitF



[Oceanobacillus iheyensis]


iheyensis



3903
2-dehydro-3-deoxyphosphogluconate
UniRef100_Q8EP99

Oceanobacillus

KdgA



aldolase [Oceanobacillus iheyensis]


iheyensis



3904
2-keto-3-deoxygluconate kinase
UniRef100_Q8EPA0

Oceanobacillus

KdgK



[Oceanobacillus iheyensis]


iheyensis



3905
Transcriptional regulator
UniRef100_Q8EPA1

Oceanobacillus

KipR



[Oceanobacillus iheyensis]


iheyensis



3906



YwaD


3907
Hypothetical protein ywoD [Bacillus
UniRef100_P94574

Bacillus subtilis

YwoD




subtilis]



3908


3909
Hypothetical protein ywaC [Bacillus
UniRef100_P39583

Bacillus subtilis

YwaC




subtilis]



3910


3911



DltA


3912
Protein dltB [Bacillus subtilis]
UniRef100_P39580

Bacillus subtilis

DltB


3913
D-alanine--poly(phosphoribitol) ligase
UniRef100_P39579

Bacillus subtilis




subunit 2 [Bacillus subtilis]


3914
Protein dltD precursor [Bacillus subtilis]
UniRef100_P39578

Bacillus subtilis

DltD


3915
Putative branched-chain-amino-acid
UniRef100_P39576

Bacillus subtilis

YwaA



aminotransferase [Bacillus subtilis]


3916
Putative macrolide-efflux determinant,
UniRef100_O32203

Bacillus subtilis

YvqJ



YvgJ [Bacillus subtilis]


3917
Probable 6-phospho-beta-glucosidase
UniRef100_P46320

Bacillus subtilis

LicH



[Bacillus subtilis]


3918
PTS system, lichenan-specific IIA
UniRef100_P46319

Bacillus subtilis

LicA



component [Bacillus subtilis]


3919
PTS system, lichenan-specific IIC
UniRef100_P46317

Bacillus subtilis

LicC



component [Bacillus subtilis]


3920
PTS system, lichenan-specific IIB
UniRef100_P46318

Bacillus subtilis




component [Bacillus subtilis]


3921
Probable licABCH operon regulator
UniRef100_P46321

Bacillus subtilis

LicR



[Bacillus subtilis]


3922


3923
Putative 3-methyladenine DNA
UniRef100_P94378

Bacillus subtilis

YxlJ



glycosylase [Bacillus subtilis]


3924
Transcriptional regulator [Rhizobium
UniRef100_Q988D9

Rhizobium loti

YdhC




loti]



3925
Citrate synthase III [Bacillus subtilis]
UniRef100_P45858

Bacillus subtilis

MmgD


3926
2-methylisocitrate dehydratase [Bacillus
UniRef100_Q6HJ23

Bacillus

MmgE




thuringiensis]



thuringiensis



3927
Putative carboxyvinyl-
UniRef100_P54528

Bacillus subtilis

YqiQ



carboxyphosphonate phosphorylmutase



[Bacillus subtilis]


3928
Chromate transporter [Bacillus
UniRef100_Q6HIU6

Bacillus

YwrA




thuringiensis]



thuringiensis



3929
BH0575 protein [Bacillus halodurans]
UniRef100_Q9KFB0

Bacillus halodurans



3930



YwjA


3931
YesT protein [Bacillus subtilis]
UniRef100_O31523

Bacillus subtilis

YesT


3932



YweA


3933



YweA


3934
Drug resistance transporter Bcr/CflA
UniRef100_Q6HPJ5

Bacillus

YdgK



subfamily; possible bicyclomycin


thuringiensis




resistance protein [Bacillus




thuringiensis]



3935
YxlG protein [Bacillus subtilis]
UniRef100_P94375

Bacillus subtilis

YxlG


3936
YxlF protein [Bacillus subtilis]
UniRef100_P94374

Bacillus subtilis

YxlF


3937
YxlE protein [Bacillus subtilis]
UniRef100_P94373

Bacillus subtilis



3938


3939
Hypothetical protein yxlC [Bacillus
UniRef100_P94371

Bacillus subtilis





subtilis]



3940
RNA polymerase sigma factor sigY
UniRef100_P94370

Bacillus subtilis

SigY



[Bacillus subtilis]


3941
YxlH protein [Bacillus subtilis]
UniRef100_P94376

Bacillus subtilis

YxlH


3942
Catalase [Bacillus halodurans]
UniRef100_Q9KDA8

Bacillus

KatX






halodurans



3943
Catalase [Bacillus subtilis]
UniRef100_Q9AQQ9

Bacillus subtilis

KatA


3944
Peroxide operon transcriptional
UniRef100_Q71YY4

Listeria

PerR



regulator [Listeria monocytogenes]


monocytogenes



3945
Ferrochelatase [Oceanobacillus
UniRef100_Q8ERX9

Oceanobacillus

HemH




iheyensis]



iheyensis



3946
Hypothetical protein yhcI [Bacillus
UniRef100_P54593

Bacillus subtilis

YhcI




subtilis]



3947
Bacitracin transport ATP-binding
UniRef100_Q81AB3

Bacillus cereus

YhcH



protein bcrA [Bacillus cereus]


3948
MrsE protein [Bacillus sp.]
UniRef100_Q9RC25

Bacillus sp.



3949


3950
ABC-2 type transport system ATP-
UniRef100_Q8ZZG9

Pyrobaculum

YdbJ



binding protein [Pyrobaculum


aerophilum





aerophilum]



3951


3952
Transcriptional regulator [Bacillus
UniRef100_Q9KFL9

Bacillus halodurans





halodurans]



3953


3954
ClyA protein [Plasmid pAD1]
UniRef100_Q52055
Plasmid pAD1
IspA


3955
Lantibiotic mersacidin transporter
UniRef100_Q9KFM8

Bacillus

YgaD



system [Bacillus halodurans]


halodurans



3956


3957
Lantibiotic mersacidin [Bacillus
UniRef100_Q9KFM5

Bacillus halodurans





halodurans]



3958
Lantibiotic mersacidin modifying
UniRef100_Q9KFM4

Bacillus halodurans




enzyme [Bacillus halodurans]


3959
Pectate lyase Pel-34K [Bacillus sp. P-
UniRef100_Q8L0S6

Bacillus sp. P-

Pel



2850]

2850


3960
Hypothetical UPF0031 protein yxkO
UniRef100_P94368

Bacillus subtilis

YxkO



[Bacillus subtilis]


3961
Transport ATP-binding protein cydD
UniRef100_P94367

Bacillus subtilis

CydD



[Bacillus subtilis]


3962
Transport ATP-binding protein cydC
UniRef100_P94366

Bacillus subtilis

CydC



[Bacillus subtilis]


3963
Cytochrome d ubiquinol oxidase
UniRef100_P94365

Bacillus subtilis

CydB



subunit II [Bacillus subtilis]


3964
Cytochrome d ubiquinol oxidase
UniRef100_P94364

Bacillus subtilis

CydA



subunit I [Bacillus subtilis]


3965
Hypothetical transport protein ywcJ
UniRef100_P39608

Bacillus subtilis

YwcJ



[Bacillus subtilis]


3966
Hypothetical protein [Bacillus cereus]
UniRef100_Q735J7

Bacillus cereus



3967
Probable NAD-dependent malic
UniRef100_P54572

Bacillus subtilis

MleA



enzyme 1 [Bacillus subtilis]


3968
Malate-2H(+)/Na(+)-lactate antiporter
UniRef100_P54571

Bacillus subtilis

MleN



[Bacillus subtilis]


3969



AnsB


3970
L-asparaginase [Bacillus subtilis]
UniRef100_P26900

Bacillus subtilis

AnsA


3971



AnsR


3972
Hypothetical protein ykgB [Bacillus
UniRef100_O34499

Bacillus subtilis

YkgB




subtilis]



3973
Hypothetical sensory transduction
UniRef100_P42421

Bacillus subtilis

YxdJ



protein yxdJ [Bacillus subtilis]


3974
Hypothetical sensor-like histidine
UniRef100_P42422

Bacillus subtilis

YxdK



kinase yxdK [Bacillus subtilis]


3975
Hypothetical ABC transporter ATP-
UniRef100_P42423

Bacillus subtilis

YxdL



binding protein yxdL [Bacillus subtilis]


3976
Hypothetical protein yxdM [Bacillus
UniRef100_P42424

Bacillus subtilis

YxdM




subtilis]



3977
Hypothetical protein yxeA precursor
UniRef100_P54940

Bacillus subtilis

YxeA



[Bacillus subtilis]


3978
YxkH protein [Bacillus subtilis]
UniRef100_P94361

Bacillus subtilis

YxkH


3979
Transcriptional regulator [Bacillus
UniRef100_Q9KFA7

Bacillus

YdeL




halodurans]



halodurans



3980
Hypothetical protein orfD [Lactococcus
UniRef100_O86288

Lactococcus

YdaM




lactis]



lactis



3981
Citrate/malate transporter [Bacillus
UniRef100_P94363

Bacillus subtilis

YxkJ




subtilis]



3982
Glycerophosphodiester
UniRef100_Q8EMK9

Oceanobacillus

YhdW



phosphodiesterase [Oceanobacillus


iheyensis





iheyensis]



3983
Hypothetical protein yhjA precursor
UniRef100_O07555

Bacillus subtilis




[Bacillus subtilis]


3984


3985
YfnI [Bacillus subtilis]
UniRef100_O06487

Bacillus subtilis

YfnI


3986
Hypothetical lipoprotein yfjD precursor
UniRef100_O31555

Bacillus subtilis

YfjD



[Bacillus subtilis]


3987
YxkD protein [Bacillus subtilis]
UniRef100_P94357

Bacillus subtilis

YxkD


3988
Arginine repressor, argR [Bacillus
UniRef100_Q81II2

Bacillus cereus

AhrC




cereus]



3989
Arginine deiminase [Bacillus cereus]
UniRef100_Q73E87

Bacillus cereus

YkgA


3990
Ornithine carbamoyltransferase
UniRef100_Q73E86

Bacillus cereus

ArgF



[Bacillus cereus]


3991
Arginine/ornithine antiporter [Bacillus
UniRef100_Q73E85

Bacillus cereus

YvsH




cereus]



3992
Carbamate kinase [Bacillus cereus]
UniRef100_Q81IH8

Bacillus cereus



3993
Transcriptional regulator, Crp family
UniRef100_Q6HP25

Bacillus

Fnr



[Bacillus thuringiensis]


thuringiensis



3994
Beta-glucosidase [Oceanobacillus
UniRef100_Q8ES64

Oceanobacillus

YdhP




iheyensis]



iheyensis



3995
PTS system, lichenan-specific IIc
UniRef100_Q6D101

Erwinia

LicC



component [Erwinia carotovora]


carotovora



3996
Hypothetical protein [Bacillus
UniRef100_Q6HC13

Bacillus thuringiensis





thuringiensis]



3997



SigV


3998
Anaerobic ribonucleoside-triphosphate
UniRef100_O26387

Methanobacterium




reductase activating protein


thermoautotrophicum




[Methanobacterium




thermoautotrophicum]



3999
Hypothetical protein yxeC [Bacillus
UniRef100_P54942

Bacillus subtilis





subtilis]



4000


4001


4002
Hypothetical protein [Anabaena sp.]
UniRef100_Q9AIM4

Anabaena sp.

FtsH


4003
Peptidase T [Bacillus subtilis]
UniRef100_P55179

Bacillus subtilis

PepT


4004
Beta-fructosidase FruA [Bacillus
UniRef100_Q8GM36

Bacillus

SacA




megaterium]



megaterium



4005
Sugar transporter FruP [Bacillus
UniRef100_Q8GM37

Bacillus

YwbF




megaterium]



megaterium



4006
ABC transporter membrane-spanning
UniRef100_Q8DNN9

Streptococcus

YesQ



permease-sugar transport


pneumoniae




[Streptococcus pneumoniae]


4007
ABC transporter membrane-spanning
UniRef100_Q8DNN8

Streptococcus

YurN



permease-sugar transporter


pneumoniae




[Streptococcus pneumoniae]


4008



YesO


4009
Repressor FruR [Bacillus megaterium]
UniRef100_Q8GM38

Bacillus

RbsR






megaterium



4010
Hypothetical protein [Bacillus cereus]
UniRef100_Q738I8

Bacillus cereus



4011



YjeA


4012
Hypothetical protein yxjG [Bacillus
UniRef100_P42318

Bacillus subtilis

YxjG




subtilis]



4013
YkcB protein [Bacillus subtilis]
UniRef100_O34575

Bacillus subtilis

YkcB


4014
Putative glycosyl transferase ykcC
UniRef100_O34319

Bacillus subtilis

YkcC



[Bacillus subtilis]


4015



GalE


4016


4017


4018


4019
Hypothetical 10.1 kDa protein [Bacillus
UniRef100_O50571

Bacillus pseudofirmus





pseudofirmus]



4020


4021
Hypothetical transport protein yxjA
UniRef100_P42312

Bacillus subtilis

YxjA



[Bacillus subtilis]


4022
Catalase 2 [Bacillus subtilis]
UniRef100_P42234

Bacillus subtilis

KatE


4023
Catalase 2 [Bacillus subtilis]
UniRef100_P42234

Bacillus subtilis

KatE


4024
DNA gyrase inhibitory protein, GyrI
UniRef100_Q97DI7

Clostridium

YosT



[Clostridium acetobutylicum]


acetobutylicum



4025
Probable glycosyl hydrolase [Erwinia
UniRef100_Q6D774

Erwinia

YckE




carotovora]



carotovora



4026
PTS system, beta-glucoside-specific
UniRef100_Q9KG19

Bacillus

BglP



enzyme II, ABC component [Bacillus


halodurans





halodurans]



4027
Transcription antiterminator licT
UniRef100_P39805

Bacillus subtilis

LicT



[Bacillus subtilis]


4028
Hypothetical conserved protein
UniRef100_Q8ES40

Oceanobacillus

YkoC



[Oceanobacillus iheyensis]


iheyensis



4029
Cation ABC transporter ATP-binding
UniRef100_Q8ES39

Oceanobacillus

YkoD



protein [Oceanobacillus iheyensis]


iheyensis



4030
Hypothetical conserved protein
UniRef100_Q8ES38

Oceanobacillus

YkoE



[Oceanobacillus iheyensis]


iheyensis



4031
Transcriptional activator of extracellular
UniRef100_Q8ESZ0

Oceanobacillus

TenA



enzyme genes [Oceanobacillus


iheyensis





iheyensis]



4032
Isocitrate lyase [Bacillus cereus]
UniRef100_Q73C38

Bacillus cereus

YqiQ


4033
Malate synthase A [Bacillus cereus]
UniRef100_Q73C39

Bacillus cereus



4034


4035
Hypothetical protein yycA [Bacillus
UniRef100_P37483

Bacillus subtilis

YycA




subtilis]



4036
Bacitracin export permease protein
UniRef100_O34741

Bacillus subtilis

YtsD



bceB [Bacillus subtilis]


4037
Bacitracin export ATP-binding protein
UniRef100_O34697

Bacillus subtilis

YtsC



bceA [Bacillus subtilis]


4038
Sensor protein bceS [Bacillus subtilis]
UniRef100_O35044

Bacillus subtilis

YtsB


4039
Sensory transduction protein bceR
UniRef100_O34951

Bacillus subtilis

YtsA



[Bacillus subtilis]


4040
Beta-glucosidase [Bacillus subtilis]
UniRef100_P40740

Bacillus subtilis

BglH


4041
PTS system, beta-glucoside-specific
UniRef100_P40739

Bacillus subtilis

BglP



IIABC component [Bacillus subtilis]


4042
Hypothetical protein yxeG [Bacillus
UniRef100_P54946

Bacillus subtilis

YxeG




subtilis]



4043
Hypothetical protein yxeI [Bacillus
UniRef100_P54948

Bacillus subtilis

YxeI




subtilis]



4044


4045


4046
Hypothetical protein yxiA precursor
UniRef100_P42293

Bacillus subtilis

YxiA



[Bacillus subtilis]


4047
ATP-dependent RNA helicase dbpA
UniRef100_P42305

Bacillus subtilis

DeaD



[Bacillus subtilis]


4048
Hypothetical protein OB2811
UniRef100_Q8EMN1

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


4049
Hypothetical protein OB2810
UniRef100_Q8EMN2

Oceanobacillus iheyensis




[Oceanobacillus iheyensis]


4050



YxiD


4051


4052
BH4015 protein [Bacillus halodurans]
UniRef100_Q9K5S3

Bacillus

YxiB






halodurans



4053
Pyrimidine-nucleoside phosphorylase
UniRef100_P39142

Bacillus subtilis

Pdp



[Bacillus subtilis]


4054
Pyrimidine nucleoside transport protein
UniRef100_P39141

Bacillus subtilis

NupC



[Bacillus subtilis]


4055
Deoxyribose-phosphate aldolase
UniRef100_P39121

Bacillus subtilis

Dra



[Bacillus subtilis]


4056
Deoxyribonucleoside regulator [Bacillus
UniRef100_P39140

Bacillus subtilis

DeoR




subtilis]



4057
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81X49

Bacillus anthracis



4058
YdaJ protein [Bacillus subtilis]
UniRef100_P96584

Bacillus subtilis

YdaJ


4059
YdaK protein [Bacillus subtilis]
UniRef100_P96585

Bacillus subtilis

YdaK


4060
YdaL protein [Bacillus subtilis]
UniRef100_O31487

Bacillus subtilis

YdaL


4061
YdaM protein [Bacillus subtilis]
UniRef100_P96587

Bacillus subtilis

YdaM


4062
YdaN protein [Bacillus subtilis]
UniRef100_O31488

Bacillus subtilis

YdaN


4063
Valine-pyruvate aminotransferase
UniRef100_Q8YTB2

Anabaena sp.

AlaT



[Anabaena sp.]


4064
Hypothetical protein [Leifsonia xyli]
UniRef100_Q6AEW0

Leifsonia xyli



4065
Hypothetical protein [Leifsonia xyli]
UniRef100_Q6AEW1

Leifsonia xyli

YwfB


4066
Similarities with putative carboxylase
UniRef100_Q7NA29

Photorhabdus

YwfE



[Photorhabdus luminescens]


luminescens



4067
Transporter, Drug/Metabolite Exporter
UniRef100_Q63AK7

Bacillus cereus

YoaV



family [Bacillus cereus ZK]

ZK


4068
Probable fructose-bisphosphate
UniRef100_P42420

Bacillus subtilis

FbaB



aldolase 2 [Bacillus subtilis]


4069
IolI protein [Bacillus subtilis]
UniRef100_P42419

Bacillus subtilis

IolI


4070
IolH protein [Bacillus subtilis]
UniRef100_P42418

Bacillus subtilis

IolH


4071
Myo-inositol dehydrogenase [Bacillus
UniRef100_Q6B6R7

Bacillus subtilis

Idh




subtilis]



4072
Myo-inositol transport protein [Bacillus
UniRef100_P42417

Bacillus subtilis

IolF




subtilis]



4073
IolE protein [Bacillus subtilis]
UniRef100_P42416

Bacillus subtilis

IolE


4074
Probable malonic semialdehyde
UniRef100_P42415

Bacillus subtilis

IolD



oxidative decarboxylase [Bacillus




subtilis]



4075
Protein iolC [Bacillus subtilis]
UniRef100_P42414

Bacillus subtilis

IolC


4076
IolB protein [Bacillus subtilis]
UniRef100_P42413

Bacillus subtilis

IolB


4077
Probable methylmalonate-
UniRef100_P42412
acylating
MmsA



semialdehyde dehydrogenase



[acylating] [Bacillus subtilis]


4078
DNA-binding protein iolR [Bacillus
UniRef100_P46337

Bacillus subtilis

IolR




subtilis]



4079
IolS protein [Bacillus subtilis]
UniRef100_P46336

Bacillus subtilis

IolS


4080
UPI00003CB380 UniRef100 entry
UniRef100_UPI00003CB380

GlpQ


4081
Putative glycerol-3-phosphate
UniRef100_Q6GJY0

Staphylococcus

GlpT



transporter [Staphylococcus aureus]


aureus



4082
Chaperone protein htpG [Bacillus
UniRef100_P46208

Bacillus subtilis

HtpG




subtilis]



4083



YfmM


4084
Hypothetical protein yxeH [Bacillus
UniRef100_P54947

Bacillus subtilis

YxeH




subtilis]



4085
Glycerol dehydrogenase [Clostridium
UniRef100_Q97IL4

Clostridium

AraM




acetobutylicum]



acetobutylicum



4086
Phosphoserine phosphatase family
UniRef100_Q97IL5

Clostridium

YkrX



protein [Clostridium acetobutylicum]


acetobutylicum



4087
BH0833 protein [Bacillus halodurans]
UniRef100_Q9KEL9

Bacillus halodurans



4088



YxeB


4089


4090
BH3956 protein [Bacillus halodurans]
UniRef100_Q9K5Y0

Bacillus

YraH






halodurans



4091


4092
Ferrous iron transport protein B
UniRef100_Q632N2

Bacillus cereus ZK




[Bacillus cereus ZK]


4093
Lmo2104 protein [Listeria
UniRef100_Q929R6

Listeria monocytogenes





monocytogenes]



4094
YfiQ protein [Bacillus subtilis]
UniRef100_O31559

Bacillus subtilis

YfiQ


4095
ABC transporter permease protein
UniRef100_Q81EI1

Bacillus cereus

YxdM



[Bacillus cereus]


4096
ABC transporter ATP-binding protein
UniRef100_Q81D39

Bacillus cereus

YxdL



[Bacillus cereus]


4097
Sensor histidine kinase [Bacillus cereus
UniRef100_Q63AY4

Bacillus cereus

YxdK



ZK]

ZK


4098
UPI00003CBD9F UniRef100 entry
UniRef100_UPI00003CBD9F

YxdJ


4099
Hypothetical protein [Bacillus cereus]
UniRef100_Q72X82

Bacillus cereus



4100
YoaH [Bacillus subtilis]
UniRef100_O34576

Bacillus subtilis

YoaH


4101
Amino acid transporter [Bacillus
UniRef100_Q9K609

Bacillus

YflA




halodurans]



halodurans



4102
Alanine dehydrogenase [Bacillus
UniRef100_Q9KAF8

Bacillus

Ald




halodurans]



halodurans



4103
Hypothetical family 53 glycosyl
UniRef100_O07013

Bacillus subtilis

YvfO



hydrolase yvfO precursor [Bacillus




subtilis]



4104
Hypothetical protein yvfN [Bacillus
UniRef100_O07012

Bacillus subtilis

LacA




subtilis]



4105
Hypothetical protein yvfM [Bacillus
UniRef100_O07011

Bacillus subtilis

YvfM




subtilis]



4106
Maltose/maltodextrin transport system
UniRef100_Q9KBA8

Bacillus

YvfL



[Bacillus halodurans]


halodurans



4107
Hypothetical protein yvfK [Bacillus
UniRef100_O07009

Bacillus subtilis

YvfK




subtilis]



4108



LacR


4109
Galactokinase [Streptococcus gordonii]
UniRef100_Q840N8

Streptococcus

GalK






gordonii



4110
UDP-glucose 4-epimerase [Listeria
UniRef100_Q928B6

Listeria innocua

GalE




innocua]



4111
UPI00003CB9B6 UniRef100 entry
UniRef100_UPI00003CB9B6

GalT


4112
Transcriptional repressor of the xylose
UniRef100_Q9KDW7

Bacillus

XylR



operon [Bacillus halodurans]


halodurans



4113



GntR


4114



GntK


4115



GntP


4116



GntZ


4117
Alcohol dehydrogenase [Bacillus
UniRef100_Q818A4

Bacillus cereus

GbsB




cereus]



4118
Alkyl hydroperoxide reductase subunit
UniRef100_P80239

Bacillus subtilis

AhpC



C [Bacillus subtilis]


4119
NADH dehydrogenase [Bacillus subtilis]
UniRef100_P42974

Bacillus subtilis

AhpF


4120


4121


4122


4123



YvfR


4124
Hypothetical protein yvfS [Bacillus
UniRef100_O07017

Bacillus subtilis

YvfS




subtilis]



4125



YvfT


4126
Hypothetical protein yvfU [Bacillus
UniRef100_O07019

Bacillus subtilis

YvfU




subtilis]



4127


4128


4129



YtrE


4130


4131


4132


4133


4134
Hypothetical protein [Desulfotalea
UniRef100_Q6ARQ4

Desulfotalea psychrophila





psychrophila]



4135
Hypothetical protein [Desulfotalea
UniRef100_Q6ARQ5

Desulfotalea psychrophila





psychrophila]



4136


4137


4138
Putative zinc metallopeptidase
UniRef100_Q894N2

Clostridium tetani




[Clostridium tetani]


4139
Type I restriction-modification system
UniRef100_Q6MH63

Bdellovibrio bacteriovorus




restriction subunit [Bdellovibrio




bacteriovorus]



4140
Lin0523 protein [Listeria innocua]
UniRef100_Q92ED7

Listeria innocua



4141


4142
Hypothetical protein CAC1662
UniRef100_Q97IH9

Clostridium acetobutylicum




[Clostridium acetobutylicum]


4143
Type I restriction-modification system,
UniRef100_Q72BC7

Desulfovibrio vulgaris




M subunit [Desulfovibrio vulgaris]


4144


4145


4146


4147
UPI00002C7192 UniRef100 entry
UniRef100_UPI00002C7192


4148
Cassette chromosome recombinase B
UniRef100_Q8RPD2

Staphylococcus

SpoIVCA



[Staphylococcus aureus]


aureus



4149
Hypothetical UPF0247 protein yyda
UniRef100_Q45601

Bacillus subtilis

YydA



[Bacillus subtilis]


4150


4151


4152
YycN protein [Bacillus subtilis]
UniRef100_O32293

Bacillus subtilis

YycN


4153


4154
UPI00003CA59D UniRef100 entry
UniRef100_UPI00003CA59D


4155
Hypothetical serine protease yyxA
UniRef100_P39668

Bacillus subtilis

YyxA



[Bacillus subtilis]


4156
YycJ protein [Bacillus subtilis]
UniRef100_Q45611

Bacillus subtilis

YycJ


4157
YycI protein [Bacillus subtilis]
UniRef100_Q45612

Bacillus subtilis

YycI


4158
YycH protein [Bacillus subtilis]
UniRef100_Q45613

Bacillus subtilis

YycH


4159
Sensor protein yycG [Bacillus subtilis]
UniRef100_Q45614

Bacillus subtilis

YycG


4160
Transcriptional regulatory protein yycF
UniRef100_P37478

Bacillus subtilis

YycF



[Bacillus subtilis]


4161
Phosphohydrolase [Bacillus cereus]
UniRef100_Q81103

Bacillus cereus



4162
Adenylosuccinate synthetase [Bacillus
UniRef100_P29726

Bacillus subtilis

PurA




subtilis]



4163


4164
Replicative DNA helicase [Bacillus
UniRef100_P37469

Bacillus subtilis

DnaC




subtilis]



4165
Hypothetical protein yycD [Bacillus
UniRef100_P37480

Bacillus subtilis





subtilis]



4166
Hypothetical protein yyzB [Bacillus
UniRef100_O32296

Bacillus subtilis





subtilis]



4167
Hypothetical transport protein yycB
UniRef100_P37482

Bacillus subtilis

YycB



[Bacillus subtilis]


4168
N-acetylglucosamine-6-phosphate
UniRef100_Q9KFQ7

Bacillus

NagA



deacetylase [Bacillus halodurans]


halodurans



4169



NagB


4170
Transcriptional regulator [Bacillus
UniRef100_Q9KFQ9

Bacillus

YvoA




halodurans]



halodurans



4171
Hypothetical protein VP0543 [Vibrio
UniRef100_Q87S81

Vibrio

YbbI




parahaemolyticus]



parahaemolyticus



4172
PTS system, n-acetylglucosamine-
UniRef100_Q9KF24

Bacillus

NagP



specific enzyme II, ABC component


halodurans




[Bacillus halodurans]


4173
50S ribosomal protein L9 [Bacillus
UniRef100_P37437

Bacillus subtilis

RplI




subtilis]



4174
Hypothetical protein yybT [Bacillus
UniRef100_P37484

Bacillus subtilis

YybT




subtilis]



4175
Hypothetical protein yybS [Bacillus
UniRef100_P37485

Bacillus subtilis

YybS




subtilis]



4176
Spore coat protein F precursor [Bacillus
UniRef100_P23261

Bacillus subtilis

CotF




subtilis]



4177
Universal stress protein, Usp family
UniRef100_Q6HIV0

Bacillus

YxiE



[Bacillus thuringiensis]


thuringiensis



4178
Sulfate permease [Bacillus
UniRef100_Q6HIU9

Bacillus

YbaR




thuringiensis]



thuringiensis



4179
Hypothetical transport protein yybO
UniRef100_P37489

Bacillus subtilis

YybO



[Bacillus subtilis]


4180
30S ribosomal protein S18 [Bacillus
UniRef100_P21475

Bacillus subtilis





subtilis]



4181
Single-strand binding protein [Bacillus
UniRef100_P37455

Bacillus subtilis

Ssb




subtilis]



4182
30S ribosomal protein S6 [Bacillus
UniRef100_P21468

Bacillus subtilis





subtilis]



4183
GTP-dependent nucleic acid-binding
UniRef100_P37518

Bacillus subtilis

YyaF



protein engD [Bacillus subtilis]


4184
Lin2921 protein [Listeria innocua]
UniRef100_Q926W9

Listeria innocua



4185
Hypothetical protein yyaD [Bacillus
UniRef100_P37520

Bacillus subtilis

YyaD




subtilis]



4186
Hypothetical protein yyaC [Bacillus
UniRef100_P37521

Bacillus subtilis

YyaC




subtilis]



4187
Stage 0 sporulation protein J [Bacillus
UniRef100_P26497

Bacillus subtilis

Spo0J




subtilis]



4188



Soj


4189
Hypothetical protein [Burkholderia
UniRef100_Q63L10

Burkholderia

YdfG




pseudomallei K96243]



pseudomallei






K96243


4190
RNA polymerase sigma factor, ECF
UniRef100_Q63BC0

Bacillus cereus

SigM



subfamily [Bacillus cereus ZK]

ZK


4191
YyaA protein [Bacillus subtilis]
UniRef100_P37524

Bacillus subtilis

YyaA


4192
Methyltransferase gidB [Bacillus
UniRef100_P25813

Bacillus subtilis

GidB




subtilis]



4193
Glucose inhibited division protein A
UniRef100_P25812

Bacillus subtilis

GidA



[Bacillus subtilis]


4194
Probable tRNA modification GTPase
UniRef100_P25811

Bacillus subtilis

ThdF



trmE [Bacillus subtilis]


4195
Jag protein [Bacillus subtilis]
UniRef100_Q01620

Bacillus subtilis

Jag


4196



SpoIIIJ


4197
Ribonuclease P protein component
UniRef100_P25814

Bacillus subtilis

RnpA



[Bacillus subtilis]
















TABLE 2







Features of the Bacillus licheniformis genome and comparison with genomes of other Bacillus species.













Feature

B. licheniformis


B. subtilis
a


B. halodurans
b


Oceanobacillus iheyensis
c


B. anthracis
d


B. cereus
e

















Chromosome Size (bp)
4,222,336
4,214,630
4,202,352
3,630,528
5,227,293
5,426,909


G + C content (mol%)
46.2
43.5
43.7
35.7
35.4
35.4


Protein coding sequences
4197
4106
4066
3496
5508
5366


Average length (bp)
873
896
879
883
800
835


Percent of coding region
87
87
85
85
84
84


Ribosomal RNA operons
7
10
8
7
11
13


Number of tRNAs
81
86
78
69
95
108


Phage-associated genes
71
268
42
27
62
124


Transposase genes of IS-elements
10
0
93
14
18
10






aKunst, F., Ogasawara, N, Mozser, I., Albertini, A. M., Alloni, G., Azebedo, V., Bertero, M. G., Bessieres, P., Bolotin, A., and Borchert, S. et al. (1997) Nature 390, 249-256.




bTakami, H., Nakasone, K., Takaki, Y., Maeno, G., Sasaki, R., Masui, N., Fuji, F., Hirama, C., Nakamura, Y., Ogasawara, N. et al. (2000) Nucleic Acids Res. 28, 4317-4331.




cTakami, H., Takaki, Y., and Uchiyama, I. (2002) Nucleic Acids Res. 30, 3927-3935.




dRead, T. D., Peterson, S. N., Tourasse, N., Baillie, L. W., Paulsen, I. T., Nelson, K. E., Tettelin, H., Fouts, D. E., Eisen, J. A., and Gill, S. R. et al. (2003) Nature 423, 81-86.




eIvanova, N, Sorokin, A., Anderson, I, Galleron, N., Candelon, B., Kapatral, V., Bhattacharyya, A., Reznik, G., Mikhailova, N., and Lapidus, A. et al. (2003) Nature 423, 87-91.














TABLE 3







Extracellular proteins predicted in the Bacillus licheniformis genome












SEQ ID
Gene
Signal



B. subtilis



NO.
Name
Peptide Location
Putative Product
Putative Function
Gene















11
dacA
[1-32]
D-alanyl-D-alanine
“Molecular Function: serine
DacA





carboxypeptidase (penicillin-
carboxypeptidase activity





binding protein 5)
(GO:0004185), Biological






Process: proteolysis and






peptidolysis (GO:006508)”











45
yabE
[1-32]
conserved hypothetical containing domain DUF348 YabE
YabE












67
divlC
[1-66]
cell-division initiation protein
“required for both vegetative and
DivlC






sporulation septum formation,






Biological Process: cell cycle






(GO:0007049)”


157
BL01016
[1-28]
N-acetylmuramoyl-L-alanine
“cell wall hydrolase, Molecular
CwlD





amidase
Function: N-acetylmuramoyl-L-






alanine amidase activity






(GO:0008745), Biological






Process: peptidoglycan






catabolism (GO:0009253)”


159
gerD
[1-27]
GerD
“germination response to L-
GerD






alanine and to the combination






of glucose, fructose, L-






asparagine, and KCl (early






stage),”


161
ybaN
[1-42]
“Polysaccharide
“Biological Process:
YbaN





deacetylase, Carbohydrate
carbohydrate metabolism





Esterase Family 4”
(GO:0005975), Molecular






Function: hydrolase activity,






acting on carbon-nitrogen (but






not peptide) bonds






(GO:0016810)”


162
pbpX
[1-43]
penicillin-binding protein

PbpX











167
ybbC
[1-23]
conserved hypothetical protein YbbC
YbbC












168
ybbD
[1-27]
“Glycoside hydrolase, family 3”
“Molecular Function: hydrolase
YbbD






activity, hydrolyzing O-glycosyl






compounds (GO:0004553),






Biological Process: carbohydrate






metabolism (GO:0005975)”


169
ybbE
[1-22]
putative beta-lactamase YbbE

YbbE


227

[1-34]
putative ribose ABC transporter
“ribose transport,”
RbsB





(ribose-binding protein)











228
yomI
[1-32]
putative lytic transglycosylase YomI
YomI












247
penP
[1-35]
beta-lactamase precursor
“Molecular Function: aspartic-
PenP






type endopeptidase activity






(GO:0004190), Biological






Process: proteolysis and






peptidolysis (GO:0006508)”











249
tatAD
[1-35]
component of the twin-arginine
“Biological Process: protein transport





pre-protein translocation
(GO:0015031), Cellular Component: integral





pathway
to membrane (GO:0016021)”


267

[1-26]
“putative Proteinase inhibititor I4,
Molecular Function: serine-type





serpin”
endopeptidase inhibitor activity






(GO:0004867)












268
BL01663
[1-22]
Pectin lyase-like

YbdN


287
BL01793
[1-31]
putative lipoprotein

YcdA


303
yvbX
[1-33]
“conserved protein, Glycoside
“Molecular Function: catalytic
YvbX





Hydrolase Family 18, YvbX”
activity (GO:0003824), Biological






Process: carbohydrate






metabolism (GO:0005975)”











304

[1-27]
“Chitinase precursor, Glycoside
“Molecular Function: catalytic activity





Hydrolase Family 18”
(GO:0003824), Biological Process:






carbohydrate metabolism (GO:0005975)”











312
yvcE
[1-31]
“putative peptidoglycan hydrolase, DL-endopeptidase II family”
YvcE












348
yckB
[1-34]
putative extracellular solute-
“Molecular Function: transporter
YckB





binding protein YckB
activity (GO:0005215), Biological






Process: transport






(GO:0006810), Cellular






Component: periplasmic space






(sensu Gram-negative Bacteria)






(GO:0030288)”


353
nucA
[1-37]
nuclease NucA

NucA


359
BL01722
[1-34]
putative extracellular solute-
“Molecular Function: transporter
YvfK





binding protein
activity (GO:0005215), Biological






Process: transport






(GO:0006810)”


373

[1-25]
hypothetical protein


382
BL01829
[1-25]
“putative extracellular solute-
“Molecular Function: transporter
YckK





binding protein, family 3”
activity (GO:0005215), Biological






Process: transport






(GO:0006810), Cellular






Component: periplasmic space






(sensu Gram-negative Bacteria)






(GO:0030288)”


403
BL01746
[1-28]
ribose ABC transporter (ribose-
“ribose transport,”
RbsB





binding protein)


409
phy
[1-30]
phytase
“hydrolysis of phytate into
Phy






inorganic phosphate and myo-






inositol,”


426
yclQ
[1-25]
Periplasmic binding protein
“Molecular Function: iron ion
YclQ






transporter activity






(GO:0005381), Biological






Process: high affinity iron ion






transport (GO:0006827)”


438

[1-26]
hypothetical protein











472
BL02821
[1-34]
Cell envelope-related transcriptional attenuator
YvhJ


483

[1-42]
hypothetical protein


513
ydcC
[1-28]
conserved membrane protein YdcC
YdcC












552
yrhM
[1-68]
YrhM

YrhM











558
ywpE
[1-26]
conserved protein YwpE
“Biological Process: biosynthesis






(GO:0009058), Molecular Function:






transferase activity (GO:0016740)”


576

[1-34]
putative transporter
“Molecular Function: transporter activity






(GO:0005215), Biological Process: transport






(GO:0006810), Cellular Component:






membrane (GO:0016020)”












605
yjeAA
[1-37]
conserved hypothetical protein

YjeA


607
amyL
[1-30]
“alpha amylase, Glycoside
“Molecular Function: alpha-
TreA





Hydrolase Family 13”
amylase activity (GO:0004556),






Biological Process: carbohydrate






metabolism (GO:0005975)”


610
yvdG
[1-28]
putative maltose/maltodextrin
“Molecular Function: transporter
YvdG





transport system substrate-
activity (GO:0005215), Biological





binding protein
Process; transport






(GO:0006810)”


619

[1-37]
hypothetical protein


620

[1-28]
hypothetical protein


621
ydjM
[1-41]
YdjM

YdjM


622
ydjN
[1-21]
YdjN

YdjN


660
yerB
[1-28]
conserved protein YerB

YerB


665
yerH
[1-26]
YerH

YerH











683
BL05063
[1-35]
mannan endo-1,4-beta-
Degradation of mannan polysaccharides





mannosidase


733

[1-29]
putative carboxypeptidase
“Molecular Function: carboxypeptidase A






activity (GO:0004182), Biological Process:






proteolysis and peptidolysis (GO:0006508)”












773
yfkD
[1-26]
conserved protein YfkD

YfkD


776
BL03088
[1-24]
“Polysaccharide deacetylase,
“Biological Process:
YfjS





Carbohydrate Esterase Family
carbohydrate metabolism





4”
(GO:0005975), Molecular






Function: hydrolase activity,






acting on carbon-nitrogen (but






not peptide) bonds






(GO:0016810)”


784
yfjL
[1-33]
putative transporter YfjL
“Molecular Function: transporter
YfjL






activity (GO:0005215), Biological






Process: transport






(GO:0006810), Cellular






Component: membrane






(GO:0016020)”


787
BL03096
[1-26]
hypothetical protein


813
BL03024
[1-26]
“putative extracellular solute-
“Molecular Function: transporter
YtcQ





binding protein, family 1”
activity (GO:0005215), Biological






Process: transport






(GO:0006810)”


841
appAC
[1-30]
oligopeptide ABC transporter
“required for initiation of
OppA





(binding protein)
sporulation, competence






development, and oligopeptide






transport, Molecular Function:






transporter activity






(GO:0005215), Biological






Process: transport






(GO:0006810)”











866
ssuA
[1-24]
aliphatic sulfonate ABC transporter (binding lipoprotein)
SsuA












876

[1-23]
hypothetical protein




886

[1-23]
hypothetical protein


892

[1-28]
hypothetical protein

YhcC


900
BL03178
[1-27]
putative lipoprotein

YhcJ


902
BL03161
[1-31]
hypothetical protein

YhcM


904
BL03176
[1-29]
hypothetical protein

YhcN


905
BL03175
[1-30]
hypothetical protein

YhcP


934
lytE
[1-26]
cell wall hydrolase phosphalase-
“cell wall lytic activity, Biological
LytE





associated protein
Process: cell wall catabolism






(GO:0016998), Biological






Process: cell wall catabolism






(GO:0016998)”











937

[1-26]
putative glucose dehydrogenase













965
BL02841
[1-41]
“Polysaccharide deacetylase,
“Biological Process:
YheN





Carbohydrate Esterase Family
carbohydrate metabolism





4”
(GO:0005975), Molecular






Function: hydrolase activity,






acting on carbon-nitrogen (but






not peptide) bonds






(GO:0016810)”


996
prsA
[1-30]
molecular chaperone PrsA
“essential for the stability of
PrsA






secreted proteins at stages






following translocation across






the membrane, Molecular






Function: isomerase activity






(GO:0016853)”


1001
BL02907
[1-31]
hypothetical protein

YhaH


1032
yhfQ
[1-32]
putative transferase
“Biological Process; metabolism
YhfQ






(GO:0008152), Molecular






Function: transferase activity






(GO:0016740)”


1044
epr
[1-27]
extracellular serine protease
“Molecular Function: subtilase
Epr






activity (GO:0004289), Biological






Process: proteolysis and






peptidolysis






(GO:0006508), Molecular






Function: subtilase activity






(GO:0004289), Biological






Process: proteolysis and






peptidolysis (GO:0006508)”


1061

[1-24]
hypothetical protein


1063
msmE
[1-29]
multiple sugar-binding protein
“Molecular Function: transporter
MsmE





MsmE
activity (GO:0005215), Biological






Process: transport






(GO:0006810)”


1073
BL01323
[1-28]
conserved hypothetical protein

LytB


1077
yvgL
[1-29]
molybdate transport system
“Molecular Function: molybdate-
YvgL





substrate-binding protein
transporting ATPase activity






(GO:0015412), Biological






Process: molybdate ion transport






(GO:0015689)”


1113
BL01309
[1-24]
Short-chain
“Biological Process: metabolism
YcdF





dehydrogenase/reductase SDR
(GO:0008152), Molecular






Function: oxidoreductase activity






(GO:0016491)”


1150
appA
[1-32]
oligopeptide ABC transporter
“oligopeptide transport, Molecular
AppA





(oligopeptide-binding protein)
Function: transporter activity






(GO:0005215), Biological






Process: transport






(GO:0006810)”


1156
oppA
[1-29]
oligopeptide ABC transporter
“required for initiation of
OppA





(binding protein)
sporulation, competence






development, and oligopeptide






transport, Molecular Function:






transporter activity






(GO:0005215), Biological






Process: transport






(GO:0006810)”


1165
BL03332
[1-31]
conserved hypothetical protein

YflP


1216
abnAA
[1-26]
Glycoside Hydrolase Family 43
“degradation of plant cell wall
AbnA






polysaccharide, Molecular






Function: hydrolase activity,






hydrolyzing O-glycosyl






compounds (GO:0004553),






Biological Process: carbohydrate






metabolism (GO:0005975)”


1219

[1-30]
hypothetical protein


1226
BL01957
[1-23]
conserved hypothetical protein

YoeB


1227
BL01953
[1-26]
Peptidoglycan-binding protein
“Biological Process: cell wall
YocH






catabolism






(GO:0016998), Biological






Process: cell wall catabolism






(GO:0016998)”


1283
yesO
[1-28]
putative transport system
“Molecular Function: transporter
YesO





substrate-binding protein YesO
activity (GO:0005215), Biological






Process: transport






(GO:0006810)”











1291
yesW
[1-33]
putative polysaccharide lyase family 11 protein
YesW












1299
lplA
[1-32]
lipoprotein
“Molecular Function: transporter
LplA






activity (GO:0005215), Biological






Process: transport






(GO:0006810)”


1311
dppE
[1-27]
dipeptide ABC transporter
“Molecular Function: transporter
DppE





(dipeptide-binding protein)
activity (GO:0005215), Biological






Process: transport






(GO:0006810)”


1319
pelI
[1-25]
“pectate lyase family 1, PelI”

Pel


1332

[1-30]
hypothetical protein


1341

[1-27]
hypothetical protein


1345

[1-27]
hypothetical protein

YonS


1368

[1-28]
hypothetical protein


1415
pbpC
[1-30]
penicillin-binding protein 3
Molecular Function: penicillin
PbpC






binding (GO:0008658)


1448

[1-28]
hypothetical protein


1475
BL05139
[1-27]
glycerophosphoryl diester
“hydrolysis of deacylated
GlpQ





phosphodiesterase
phospholipids, Biological






Process: glycerol metabolism






(GO:0006071), Molecular






Function: glycerophosphodiester






phosphodiesterase activity






(GO:0008889)”


1479

[1-27]
hypothetical protein


1493

[1-27]
hypothetical protein


1496
BL03556
[1-25]
putative Cell wall hydrolase

YkvT


1498
ykvV
[1-28]
hypothetical protein
Contains thioredoxin domain 2
YkvV


1501

[1-25]
hypothetical protein


1512
ykwC
[1-24]
Hypothetical oxidoreductase

YkwC


1513
BL03646
[1-27]
Allergen V5/Tpx-1 related
Cellular Component:
YkwD






extracellular (GO:0005576)


1527

[1-28]
hypothetical protein


1553
BL01601
[1-32]
“putative secretion protein,
“Molecular Function: protein
YknX





protein transporter”
transporter activity






(GO:0008565), Biological






Process: protein secretion






(GO:0009306), Cellular






Component: membrane






(GO:0016020)”


1565

[1-40]
hypothetical protein


1576
ykyA
[1-29]
Hypothetical protein ykyA

YkyA


1601
BL02997
[1-27]
hypothetical protein

YlaJ


1617
ylbC
[1-34]
conserved hypothetical protein
Cellular Component:
YlbC





YlbC
extracellular (GO:0005576)


1625
ylbL
[1-29]
YlbL
Molecular Function: protein
YlbL






binding (GO:0005515)


1635
ftsL
[1-59]
cell-division protein
“septum formation (early stage),”
FtsL


1636
pbpB
[1-42]
penicillin-binding protein 2B
“formation of the cell-division
PbpB






septum (late stage),”


1644
divlB
[1-57]
cell-division initiation protein
“probably involved in stabilizing
DivlB






or promoting the assembly of the






division complex (septum






formation),”


1645
BL02246
[1-29]
conserved hypothetical protein

YlxW


1646
BL02248
[1-30]
hypothetical protein

YlxX


1650
bprA
[1-28]
bacillopeptidase F
“Molecular Function: subtilase
Bpr






activity (GO:0004289), Biological






Process: proteolysis and






peptidolysis (GO:0006508)”


1651
bprB
[1-31]
bacillopeptidase F
“Molecular Function: subtilase
Bpr






activity (GO:0004289), Biological






Process: proteolysis and






peptidolysis (GO:0006508)”


1752
fliL
[1-32]
flagellar protein
“required for flagellar
FliL






formation, Biological Process:






ciliary/flagellar motility






(GO:0001539), Biological






Process: chemotaxis






(GO:0006935), Cellular






Component: flagellar basal body






(sensu Bacteria) (GO:0009425)”











1781
celA
[1-34]
Glycoside Hydrolase Family 9
“Molecular Function: hydrolase activity,






hydrolyzing O-glycosyl compounds






(GO:0004553), Biological Process:






carbohydrate metabolism (GO:0005975)”


1783
celC
[1-34]
Glycoside Hydrolase Family 5
“Molecular Function: hydrolase activity,






hydrolyzing O-glycosyl compounds






(GO:0004553), Biological Process:






carbohydrate metabolism (GO:0005975)”


1784
celD
[1-32]
Glycoside Hydrolase family 5
“Molecular Function: hydrolase activity,






hydrolyzing O-glycosyl compounds






(GO:0004553), Biological Process:






carbohydrate metabolism (GO:0005975)”












1820
BL03655
[1-31]
conserved hypothetical protein

YmdA


1839

[1-29]
hypothetical protein


1904
yoaO
[1-38]
YoaO

YoaO


1914
yoaW
[1-26]
YoaW

YoaW


1926

[1-38]
phage-like protein


1929
nucB
[1-34]
nuclease

NucB


1931
BL05188
[1-29]
Peptidoglycan-binding protein
Biological Process: cell wall
YneA






catabolism (GO:0016998)


1946
yneN
[1-29]
putative thiol: disulfide
“Molecular Function: electron
YneN





interchange protein YneN
transporter activity






(GO:0005489), Biological






Process: electron transport






(GO:0006118)”


1983
bglC
[1-50]
“endo-1,4-beta-glucanase,
“Molecular Function: hydrolase
BglC





Glycoside hydrolase Family 5”
activity, hydrolyzing O-glycosyl






compounds (GO:0004553),






Biological Process: carbohydrate






metabolism (GO:0005975),”











1990
ywoF
[1-22]
“Polysaccharide Lyase Family 9, YwoF”
YwoF












1995
BL00297
[1-27]
conserved hypothetical protein

YddR


2011
yjmF
[1-31]
Short-chain
“Biological Process: metabolism
YjmF





dehydrogenase/reductase SDR
(GO:0008152), Molecular






Function: oxidoreductase activity






(GO:0016491)”


2016
dctB
[1-29]
possible C4-dicarboxylate
“, Biological Process: transport
DctB





binding protein
(GO:0006810), Cellular






Component: periplasmic space






(sensu Gram-negative Bacteria)






(GO:0030288)”


2028
dacC
[1-29]
penicillin-binding protein (D-
“Molecular Function: serine
DacC





alanyl-D-alanine
carboxypeptidase activity





carboxypeptidase)
(GO:0004185), Biological






Process: proteolysis and






peptidolysis (GO:0006508)”


2060

[1-37]
hypothetical protein











2072
BL00899
[1-28]
“Spore germination B3 GerAC like, C-terminal”
YndF












2105

[1-33]
conserved hypothetical protein




2108
BL01303
[1-26]
Peptidoglycan-binding protein
“Biological Process: cell wall
YocH






catabolism






(GO:0016998), Biological






Process: cell wall catabolism






(GO:0016998)”


2123

[1-36]
hypothetical protein


2132
BL01404
[1-24]
hypothetical protein

YoqH


2152
yvgO
[1-25]
conserved protein YvgO

YvgO


2160
lytF
[1-27]
gamma-D-glutamate-meso-
“cell wall lytic activity, Biological
LytF





diaminopimelate muropeptidase
Process: cell wall catabolism





(major autolysin) (CWBP49′)
(GO:0016998)”


2167
yoaJ
[1-26]
YoaJ

YoaJ


2177
ctpA
[1-37]
carboxy-terminal processing
“, Biological Process: proteolysis
CtpA





protease
and peptidolysis (GO:0006508),






Molecular Function: serine-type






peptidase activity






(GO:0008236)”


2180
yodJ
[1-31]
putative carboxypeptidase

YodJ


2207
ypmS
[1-34]
conserved protein YpmS

YpmS


2208
ypmR
[1-24]
conserved protein YpmR
Molecular Function: catalytic
YpmR






activity (GO:0003824)


2209
ypmQ
[1-25]
conserved hypothetical YpmQ
Biological Process: electron
YpmQ






transport (GO:0006118)











2218
ypjP
[1-30]
conserved hypothetical protein YpjP
YpjP












2266
ponA
[1-63]
“penicillin-binding
“involved in division septum
PonA





proteins, Glycosyl Transferase
formation,”





Family 51”


2271
aspB
[1-23]
aspartate aminotransferase
“Molecular Function:
AspB






transaminase activity






(GO:0008483), Biological






Process: biosynthesis






(GO:0009058)”


2272
ypmB
[1-25]
conserved protein YpmB

YpmB


2815
BL02789
[1-29]
hypothetical protein

YphF


2328
sleB
[1-34]
spore cortex-lytic enzyme

SleB


2330
ansZA
[1-18]
putative
“Biological Process: amino acid
YccC





Asparaginase/glutaminase
metabolism






(GO:0006520), Biological






Process: amino acid metabolism






(GO:0006520)”


2334
BL02228
[1-27]
putative hydrolase
Molecular Function: hydrolase
YpbG






activity (GO:0016787)











2344
yocH
[1-31]
putative peptidogycan hydrolase
“Biological Process: cell wall catabolism





YocH
(GO:0016998), Biological Process: cell wall






catabolism (GO:0016998)”












2347
BL00652
[1-32]
hypothetical protein

LytR


2348
yheN
[1-53]
“Carbohydrate Esterase Family
“Biological Process:
YheN





4, YheN”
carbohydrate metabolism






(GO:0005975), Molecular






Function: hydrolase activity,






acting on carbon-nitrogen (but






not peptide) bonds






(GO:0016810)”


2349

[1-29]
hypothetical protein


2360
dacB
[1-28]
D-alanyl-D-alanine
“required for spore cortex
DacB





carboxypeptidase (penicillin-
synthesis, Molecular Function:





binding protein 5*)
serine carboxypeptidase activity






(GO:0004185), Biological






Process: proteolysis and






peptidolysis (GO:0006508)”


2371
BL01892
[1-44]
conserved hypothetical protein

YpuD


2876
BL03279
[1-24]
conserved hypothetical protein

YpuA


2392
dacF
[1-33]
penicilin binding protein (putative
“required for spore cortex
DacF





D-alanyl-D-alanine
synthesis, Molecular Function:





carboxypeptidase)
serine carboxypeptidase activity






(GO:0004185), Biological






Process: proteolysis and






peptidolysis (GO:0006508)”











2400
BL01161
[1-28]
“Phosphotransferase system,
“Molecular Function: sugar porter activity





lactose/cellobiose-specific IIB
(GO:0005351), Biological Process:





subunit”
phosphoenolpyruvate-dependent sugar






Phosphotransferase system (GO:0009401)”












2418
lip
[1-31]
lipase
Molecular Function: catalytic
Lip






activity (GO:0003824)


2420
appAA
[1-32]
oligopeptide ABC transporter
“oligopeptide transport, Molecular
AppA





(oligopeptide-binding protein)
Function: transporter activity






(GO:0005215), Biological






Process: transport






(GO:0006810)”


2435

[1-24]
hypothetical protein


2436

[1-29]
hypothetical protein


2438
BL00808
[1-38]
hypothetical protein

YmaC


2449

[1-35]
hypothetical protein











2450

[1-31]
conserved hyopothetical protein
SsuA











2456
BL01380
[1-28]
“Phosphotransferase system,
“Molecular Function: sugar porter activity





lactose/cellobiose-specific IIB
(GO:0005351), Biological Process:





subunit”
phosphoenolpyruvate-dependent sugar






Phosphotransferase system (GO:0009401)”












2481
BL01514
[1-36]
Cell wall hydrolase/autolysin
“Molecular Function: N-
YqiI






acetylmuramoyl-L-alanine






amidase activity (GO:0008745),






Biological Process:






peptidoglycan catabolism






(GO:0009253)”


2482
yqiH
[1-33]
YqiH

YqiH


2484

[1-26]
hypothetical protein


2499
spoIIIAH
[1-32]
SpoIIIAH
“mutants block sporulation after
SpoIIIAH






engulfment,”


2505
spoIIIAB
[1-23]
SpoIIIAB
“mutants block sporulation after
SpoIIIAB






engulfment,”


2517
yqhL
[1-24]
conserved protein YqhL

YqhL


2518
opuAC
[1-31]
glycine betaine ABC transporter
“glycine betaine
OpuAC





(glycine betaine-binding protein)
transport, Molecular Function:






transporter activity






(GO:0005215), Molecular






Function: binding (GO:0005488),






Biological Process: transport






(GO:0006810)”











2528
tasA
[1-28]
translocation-dependent antimicrobial spore component
TasA












2530
yqxM
[1-44]
YqxM

YqxM


2531
yqzG
[1-24]
YqzG

YqzG


2533
comGG
[1-38]
probably part of the DNA
“required for exogenous DNA-
ComGG





transport machinery ComGG
binding,”


2546
yqgU
[1-28]
conserved protein YqgU

YqgU


2548
fhuD
[1-32]
ferrichrome ABC transporter
“Molecular Function: iron ion
FhuD





(ferrichrome-binding protein)
transporter activity






(GO:0005381), Biological






Process: high affinity iron ion






transport (GO:0006827)”


2560
yqzC
[1-33]
conserved protein YqzC

YqzC


2565
pstS
[1-33]
phosphate ABC transporter
“involved in high-affinity
PstS





(binding protein)
phosphate uptake, Molecular






Function; transporter activity






(GO:0005215), Biological






Process: transport






(GO:0006810)”


2566
pbpA
[1-44]
penicillin-binding protein 2A
“involved in the synthesis of
PbpA






peptidoglycan associated with






cell wall elongation in spore






outgrowth, Molecular Function:






penicillin binding (GO:0008658)”


2571
yqfZ
[1-40]
conserved protein YqfZ


2616
BL01411
[1-37]
conserved hypothetical protein

YqfA


2632
yqxA
[1-29]
YqxA

YqxA


2633
spoIIP
[1-55]
SpoIIP
“required for dissolution of the
SpoIIP






septal cell wall,”


2652
BL02075
[1-36]
putative lipoprotein
Molecular Function: catalytic
YqeF






activity (GO:0003824)


2697
appAB
[1-36]
oligopeptide ABC transporter
“oligopeptide transport, Molecular
AppA





(oligopeptide-binding protein)
Function: transporter activity






(GO:0005215), Biological






Process: transport






(GO:0006810)”


2706

[1-32]
putative phosphoesterase


2707
BL02428
[1-27]
carboxylesterase family
Molecular Function: catalytic
PnbA






activity (GO:0003824)


2710
BL03118
[1-28]
hypothetical protein

YndA


2712
sacC
[1-24]
Glycoside Hydrolase Family 32
“Molecular Function: hydrolase
SacC






activity, hydrolyzing O-glycosyl






compounds (GO:0004553),






Biological Process: carbohydrate






metabolism (GO:0005975)”


2729
BL00468
[1-33]
Periplasmic iron-binding protein
“Molecular Function: iron ion
YxeB






transporter activity






(GO:0005381), Biological






Process: high affinity iron ion






transport (GO:0006827)”


2745
yrrS
[1-50]
conserved protein YrrS

YrrS


2752
yrrL
[1-44]
conserved protein YrrL

YrrL











2771
yrvJ
[1-29]
N-acetylmuramoyl-L-alanine amidase YrvJ
YrvJ












2793
ymaC
[1-24]
phage-related protein YmaC

YmaC


2797
coxA
[1-27]
spore cortex protein

CoxA


2801
nadB
[1-37]
L-aspartate oxidase
“required for NAD biosynthesis,”
NadB


2818
mreC
[1-33]
cell-shape determining protein
“, Biological Process: regulation
MreC






of cell shape (GO:0008360)”


2866

[1-28]
hypothetical protein


2872
gerM
[1-34]
spore germination protein GerM
“germination (cortex hydrolysis)
GerM






and sporulation (stage II,






multiple polar septa),”


2898

[1-33]
hypothetical protein


2911
araN
[1-32]
sugar-binding protein
“L-arabinose transport, Molecular
AraN






Function: transporter activity






(GO:0005215), Biological






Process: transport






(GO:0006810)”


2916
abnAB
[1-28]
Glycoside Hydrolase Family 43
“degradation of plant cell wall
AbnA






polysaccharide, Molecular






Function: hydrolase activity,






hydrolyzing O-glycosyl






compounds (GO:0004553),






Biological Process: carbohydrate






metabolism (GO:0005975)”











2939
pelB
[1-31]
“pectate lyase, Polysaccharide Lyase Family 1”
PelB












3005
ytxE
[1-51]
YtxE
Cellular Component: outer
YtxE






membrane (sensu Gram-






negative Bacteria)






(GO:0009279)


3049
ytcQ
[1-30]
putative multiple sugar transport
“Molecular Function: transporter
YtcQ





system substrate-binding protein
activity (GO:0005215), Biological





YtcQ
Process: transport






(GO:0006810)”


3059
BL00013
[1-30]
putative lipoprotein

YusA











3081
ytlA
[1-33]
putative sulfonate transport system substrate-binding protein YtlA
YtlA












3088
ytkA
[1-29]
conserved protein YtkA

YtkA


3093
BL05310
[1-29]
Periplasmic solute binding
“Molecular Function: binding
YcdH





protein
(GO:0005488), Cellular






Component: periplasmic space






(sensu Gram-negative Bacteria)






(GO:0080288)”











3217

[1-22]
hypothetical protein
Molecular Function: catalytic activity






(GO:0003824)












3235
yuiC
[1-34]
conserved protein YuiC

YuiC


3264
yutC
[1-24]
conserved protein YutC

YutC


3266
BL02121
[1-22]
putative metallopeptidase
“Molecular Function:
YunA






metalloendopeptidase activity






(GO:0004222), Biological






Process: proteolysis and






peptidolysis (GO:0006508)”


3267

[1-32]
hypothetical protein


3269
yunB
[1-41]
conserved protein YunB

YunB


3284
yurYA
[1-40]
extracellular ribonuclease
Molecular Function: nuclease
YurI






activity (GO:0004518)











3298
yusA
[1-28]
putative ABC transport system substrate-binding protein YusA
YusA












3318
yfiY
[1-29]
ABC transport system substrate-
“Molecular Function: iron ion
YfiY





binding protein
transporter activity






(GO:0005381), Biological






Process: high affinity iron ion






transport (GO:0006827)”


3319
yusW
[1-28]
YusW

YusW


3333
gerAC
[1-25]
spore germination protein A3
“germination response to L-
GerAC





precursor
alanine and related amino acids






(earliest stage),”


3338
yvqG
[1-39]
conserved protein YvqG
“Biological Process: metabolism
YvqG






(GO:0008152), Molecular






Function: oxidoreductase activity






(GO:0016491)”


3349
yvrC
[1-37]
putative iron transport system
“Molecular Function: iron ion
YvrC





substrate-binding protein YvrC
transporter activity






(GO:0005381), Biological






Process: high affinity iron ion






transport (GO:0006827)”


3380
BL02684
[1-27]
putative Extracellular solute-
Molecular Function: transporter
YvfK





binding protein
activity (GO:0005215)











3384
BL02680
[1-31]
putative Glycoside Hydrolase Family 3
YbbD












3387
mntA
[1-28]
manganese transport system
“Molecular Function: binding
YcdH





substrate-binding protein
(GO:0005488), Cellular






Component: periplasmic space






(sensu Gram-negative Bacteria)






(GO:0030288)”


3429

[1-33]
hypothetical protein


3465

[1-32]
hypothetical protein


3492
opuCC
[1-32]
glycine betaine/carnitine/choline
“high affinity transport of glycine
OpuCC





ABC transporter
betaine, carnitine, and





(osmoprotectant-binding protein)
choline, Molecular Function:






transporter activity






(GO:0005215), Molecular






Function: binding (GO:0005488),






Biological Process: transport






(GO:0006810)”


3499
BL03470
[1-29]
“Periplasmic binding
“Molecular Function: iron ion
YfiY





protein, putative iron transporter”
transporter activity






(GO:0005381), Biological






Process: high affinity iron ion






transport (GO:00068271)”


3510

[1-26]
hypothetical protein


3547
sacB
[1-30]
“levansucrase, Glycoside
“Molecular Function: hydrolase
SacB





Hydrolase Family 68”
activity, hydrolyzing O-glycosyl






compounds (GO:0004553),






Biological Process: sugar






utilization (GO:0007587)”











3548
levB
[1-33]
“endolevanase, Glycoside Hydrolase Family 32”
YveB











3559

[1-29]
putative ribonuclease
“Molecular Function: RNA binding






(GO:0003723), Molecular Function:






endoribonuclease activity (GO:0004521)”












3578
yvpB
[1-39]
putative cysteine protease YvpB
“Molecular Function: cysteine-
YvpB






type endopeptidase activity






(GO:0004197), Biological






Process: proteolysis and






peptidolysis (GO:0006508)”











3579
yvpA
[1-29]
Polysaccharide Lyase Family 3
YvpA












3587
yvnB
[1-29]
YvnB

YvnB


3600
ctpB
[1-37]
“Peptidase S41A, C-terminal
“Biological Process: proteolysis
YvjB





protease”
and peptidolysis (GO:0006508),






Molecular Function: serine-type






peptidase activity (GO:0008236)


3602
BL03388
[1-29]
“Spectrin repeat, Rudiment single
“,”
YvcE





hybrid motif”


3643
lytC
[1-14]
N-acetylmuramoyl-L-alanine
“involved in cell separation, cell
LytC





amidase (major autolysin)
wall turnover, antibiotic-induced





(CWBP49)
lysis, motility and general cell






lysis induced by sodium






azide, Molecular Function: N-






acetylmuramoyl-L-alanine






amidase activity (GO:0008745),






Biological Process;






peptidoglycan catabolism











3644
lytB
[1-26]
modifier protein of major autolysin LytC (CWBP76)
LytB












3645
BL03297
[1-25]
membrane bound lipoprotein
involved in the secretion of
LytA






major autolysin


3646
lytR
[1-32]
membrane-bound protein
“attenuator role for lytABC and
LytR






lytR expression,”


3656
lytD
[1-29]
“N-acetylglucosaminidase (major
“involved in cell separation, cell
LytD





autolysin), Glycoside Hydrolase
wall turnover, antibiotic-induced





Family 73”
lysis and motility, Molecular






Function: amidase activity






(GO:0004040), Biological






Process: peptidoglycan






catabolism (GO:0009253)”


3670
pgdS
[1-34]
gamma-DL-glutamyl hydrolase
“gamma-DL-glutamyl hydrolase
YwtD






cleaving the gamma-glutamyl






bond only between D- and L-






glutamic acids of PGA, DL-






endopeptidase II family”


3671
ywtC
[1-25]
YwtC











3672
pgsAA
[1-47]
poly-gamma-glutamate synthesis protein
YwtB












3680
rbsB
[1-28]
ribose ABC transporter (ribose-
“ribose transport,”
RbsB





binding protein)


3738
feuA
[1-28]
iron-binding protein
“component of iron-uptake
FeuA






system, Molecular Function: iron






ion transporter activity






(GO:0005381), Biological






Process: high affinity iron ion






transport (GO:0006827)”


3751
ywmD
[1-25]
YwmD

YwmD


3752
ywmC
[1-24]
YwmC

YwmC


3753
spoIID
[1-33]
SpoIID
“required for complete
SpoIID






dissolution of the asymmetric,






septum,”


3755
ywmB
[1-30]
conserved protein YwmB

YwmB


3773
spoIIR
[1-29]
SpoIIR
required for processing of pro-
SpoIIR






sigma-E


3801
ywjE
[1-28]
putative Phospholipase
“Molecular Function: catalytic
YwjE






activity (GO:0003824), Biological






Process: metabolism






(GO:0008152)”


3808
BL03962
[1-41]
“Penicillin-binding protein,
“Molecular Function: penicillin
YwhE





Glycosyl Transferase Family 51”
binding (GO:0008658),






Biological Process: cell wall






biosynthesis (sensu Bacteria)






(GO:0009273)”


3812

[1-25]
hypothetical protein


3829
BL03904
[1-32]
hypothetical protein

YuaB


3849
vpr
[1-29]
extracellular serine protease
“Molecular Function: subtilase
Vpr






activity (GO:0004289), Biological






Process: proteolysis and






peptidolysis (GO:0006508)”


3906
ywaD
[1-31]
Putative aminopeptidase
“Biological Process: proteolysis
YwaD






and peptidolysis (GO:0006508),






Molecular Function: peptidase






activity (GO:0008233)”


3914
dltD
[1-27]
precusor DltD
D-alanine esterification of
DltD






lipoteichoic acid and wall






teichoic acid (D-alanine transfer






from undecaprenol-P to the






poly(glycerophosphate) chain of






LTA)











3920
licB
[1-24]
phosphotransferase system
“Molecular Function: sugar porter activity





(PTS) lichenan-specific enzyme
(GO:0005351), Biological Process:





IIB component
phosphoenolpyruvate-dependent sugar






phosphotransferase system (GO:0009401)”












3933
BL05372
[1-31]
hypothetical protein

YweA


3954
lanP
[1-25]
serine protease
“subtilase activity
IspA






(GO:0004289), proteolysis and






peptidolysis (GO:0006508),






lantibiotic leader peptide






processing”


3970
ansA
[1-29]
L-asparaginase
“Molecular Function:
AnsA






asparaginase activity






(GO:0004067), Biological






Process: amino acid metabolism






(GO:0006520)”


3977
BL02958
[1-25]
conserved hypothetical protein

YxeA


3978
yxkH
[1-28]
“Polysaccharide deacetylase,
“Biological Process:
YxkH





Carbohydrate Esterase Family
carbohydrate metabolism





4”
(GO:0005975), Molecular






Function: hydrolase activity,






acting on carbon-nitrogen (but






not peptide) bonds






(GO:0016810)”


3982
BL02963
[1-30]
Glycerophosphoryl diester
“Biological Process: glycerol
YhdW





phosphodiesterase
metabolism (GO:0006071),






Molecular Function:






glycerophosphodiester






phosphodiesterase activity






(GO:0008889)”


3983
BL05379
[1-28]
hypothetical protein


3996

[1-73]
hypothetical protein


4011
BL05383
[1-34]
hypothetical protein

YjeA











4016

[1-31]
hypothetical protein
Biological Process: electron transport






(GO:0006118)












4046
yxiA
[1-27]
“Glycoside hydrolase, family 43”
“Molecular Function: hydrolase
YxiA






activity, hydrolyzing O-glycosyl






compounds (GO:0004553),






Biological Process: carbohydrate






metabolism (GO:0005975)”


4058
ydaJ
[1-29]
putative Glycoside transferase

YdaJ


4080
glpQ
[1-29]
glycerophosphoryl diester
“hydrolysis of deacylated
GlpQ





phosphodiesterase
phospholipids, Biological






Process: glycerol metabolism






(GO:0006071), Molecular






Function: glycerophosphodiester






phosphodiesterase activity






(GO:0008889)”


4099
yxeA
[1-34]
conserved hypothetical protein











4103
yvfO
[1-28]
Glyccosyl Hydrolase Family 53
YvfO












4107
cycB
[1-82]
“putative extracellular solute-
“Molecular Function: transporter
YvfK





binding protein, family 1 CycB”
activity (GO:0005215), Biological






Process: transport






(GO:0006810)”


4121

[1-28]
hypothetical protein


4128

[1-34]
hypothetical protein


4130

[1-42]
hypothetical protein


4158
yycH
[1-36]
conserved hypothetical YycH

YycH
















TABLE 4







Codon Usage Table for Chromosome














Amino






# Codon
acid
Fract
/1000
Number

















GCA
A
0.230
18.394
22495



GCC
A
0.272
21.724
26567



GCG
A
0.301
24.082
29451



GCT
A
0.197
15.766
19281



TGC
C
0.662
5.113
6253



TGT
C
0.338
2.613
3195



GAC
D
0.432
22.129
27063



GAT
D
0.568
29.114
35605



GAA
E
0.704
51.721
63253



GAG
E
0.296
21.797
26657



TTC
F
0.354
16.034
19609



TTT
F
0.646
29.223
35738



GGA
G
0.316
22.644
27693



GGC
G
0.374
26.825
32806



GGG
G
0.171
12.287
15026



GGT
G
0.139
9.957
12177



CAC
H
0.386
8.412
10288



CAT
H
0.614
13.369
16350



ATA
I
0.101
7.217
8826



ATC
I
0.487
34.741
42487



ATT
I
0.412
29.376
35926



AAA
K
0.693
49.125
60078



AAG
K
0.307
21.739
26586



CTA
L
0.032
3.106
3798



CTC
L
0.152
14.848
18158



CTG
L
0.263
25.592
31298



CTT
L
0.229
22.330
27309



TTA
L
0.143
13.920
17023



TTG
L
0.181
17.639
21572



ATG
M
1.000
26.541
32458



AAC
N
0.505
19.159
23431



AAT
N
0.495
18.780
22967



CCA
P
0.112
4.189



CCC
P
0.120
4.477
5475



CCG
P
0.520
19.405
23731



CCT
P
0.248
9.236
11295



CAA
Q
0.465
16.656
20369



CAG
Q
0.535
19.179
23455



AGA
R
0.218
9.611
11754



AGG
R
0.146
6.448
7886



CGA
R
0.074
3.275
4005



CGC
R
0.253
11.173
13664



CGG
R
0.194
8.538
10441



CGT
R
0.114
5.044
6168



AGC
S
0.263
15.893
19436



AGT
S
0.063
3.820
4672



TCA
S
0.202
12.173
14887



TCC
S
0.154
9.287
11357



TCG
S
0.171
10.289
12583



TCT
S
0.147
8.865
10841



ACA
T
0.327
16.969
20752



ACC
T
0.188
9.765
11942



ACG
T
0.373
19.404
23730



ACT
T
0.112
5.833
7134



GTA
V
0.144
9.710
11875



GTC
V
0.361
24.342
29769



GTG
V
0.238
16.042
19619



GTT
V
0.257
17.354
21223



TGG
W
1.000
10.303
12600



TAC
Y
0.398
13.488
16471



TAT
Y
0.802
20.348
24885



TAA
*
0.551
1.982
2400



TAG
*
0.157
0.558
682



TGA
*
0.292
1.041
1273

















TABLE 5







Signal Peptide Codon Usage Table














Amino






Codon
acid
Fract
/1000
Number

















GCA
A
0.294
42.315
106



GCC
A
0.219
31.537
79



GCG
A
0.242
34.731
87



GCT
A
0.244
35.13
88



TGC
C
0.543
9.98
25



TGT
C
0.457
8.383
21



GAC
D
0.231
2.395
6



GAT
D
0.769
7.984
20



GAA
E
0.784
15.968
40



GAG
E
0.216
4.391
11



TTC
F
0.335
21.956
55



TTT
F
0.665
43.513
109



GGA
G
0.341
22.355
56



GGC
G
0.262
17.166
43



GGG
G
0.183
11.976
30



GGT
G
0.213
13.972
35



CAC
H
0.316
2.395
6



CAT
H
0.684
5.19
13



ATA
I
0.142
9.98
25



ATC
I
0.466
32.735
82



ATT
I
0.392
27.545
69



AAA
K
0.767
61.876
155



AAG
K
0.233
18.762
47



CTA
L
0.05
7.186
18



CTC
L
0.098
13.972
35



CTG
L
0.235
33.533
84



CTT
L
0.16
22.754
57



TTA
L
0.221
31.537
79



TTG
L
0.235
33.533
84



ATG
M
1
51.497
129



AAC
N
0.482
10.778
27



AAT
N
0.518
11.577
29



CCA
P
0.164
4.79
12



CCC
P
0.192
5.589
14



CCG
P
0.452
13.174
33



CCT
P
0.192
5.589
14



CAA
Q
0.475
11.577
29



CAG
Q
0.525
12.774
32



AGA
R
0.333
9.98
25



AGG
R
0.08
2.395
6



CGA
R
0.107
3.194
8



CGC
R
0.18
4.79
12



CGG
R
0.173
5.19
13



CGT
R
0.147
4.391
11



AGC
S
0.171
14.77
37



AGT
S
0.093
7.984
20



TCA
S
0.241
20.758
52



TCC
S
0.148
12.774
32



TCG
S
0.134
11.577
29



TCT
S
0.213
18.363
46



ACA
T
0.262
11.178
28



ACC
T
0.234
9.98
25



ACG
T
0.327
13.972
35



ACT
T
0.178
7.585
19



GTA
V
0.216
14.371
36



GTC
V
0.186
12.375
31



GTG
V
0.335
22.355
56



GTT
V
0.263
17.565
44



TGG
W
1
7.585
19



TAC
Y
0.405
5.988
15



TAT
Y
0.595
8.782
22



TAA
*
0
0
0



TAG
*
0
0
0



TGA
*
0
0
0










Example 6
Identification of Genes Induced by Glucose Limitation Using DNA Microarrays


Bacillus licheniformis ATCC 14580 was grown in duplicate shake flasks containing 100 ml of Spizizen I medium (Anagnostopoulos and Spizizen, 1961, Journal of Bacteriology 81: 741-748). The inocula for the shake flasks were obtained from 10 ml of mid-log cells (approximately 80-90 Klett units) and a 0 hour total RNA sample was extracted from 30 ml of the mid-log cells, as described below. Total cellular RNA was also purified from 10 ml of the Spizizen I shake flasks that were sampled at 2, 4, 6 and 8 hours post inoculum. All of the cell samples were mixed with two volumes of RNAProtect RNA stabilizing reagent (QIAGEN, Inc., Valencia, Calif.) for 5 minutes. The cells were subsequently pelleted at 2800×g, 4° C. for 10 minutes, the supernatant was discarded, the cell pellets were frozen at −80° C., and cellular RNA was purified using a FastRNA Pro Blue kit (QBiogene, Carlsbad, Calif.) using the protocol supplied by the manufacturer. The frozen cell pellets from each sample were resuspended in 2 ml RNAPro solution provided in the FastRNA Pro Blue kit and RNA was obtained from two lysis matrix B vials.


Sixteen replicate cDNA targets for the 0 time point and two technical replicate cDNA targets for each of the rest of the time points were prepared and hybridized to Bacillus licheniformis DNA microarrays prepared as described by Berka et al., 2002, Molecular Microbiology 43: 1331-1345. Dye swap hybridizations using the zero time point as reference were prepared as described in Hu et al. [In G. Parmigiani, E. S. Garrett, R. A Irizarry, and S. L. Zeger (eds.) The Analysis of Gene Expression Data, pp. 318-319, Springer-Verlag, New York (2003)].


The arrays were scanned with an Axon 4000B scanner and formatted for analysis with GenePix Pro version 5.0 (Axon Instruments, Inc. Redwood City, Calif.). The fluorescence intensity data from GenePix was imported directly into S-Plus ArrayAnalyzer version 2 software (Insightful Corporation, Seattle, Wash.) for statistical analysis. The raw intensity data were normalized using the lowess (locally weighted regression) function provided in S-Plus ArrayAnalyzer software, and the genes that were differentially expressed at each time point relative to the time zero reference were identified by employing a multiple comparison t-test with Bonferroni correction as outlined in the user's guide to S-Plus ArrayAnalyzer software (Insightful Corp., Seattle, Wash.). The family-wise error rate (FWER) was set at 0.1. The list of genes at each time point that passed these statistical criteria were used to query a pathway-genome database established using Pathway Tools Suite software (Karp at al., 2002. Bioinformatics 18 suppl. 1: S225-S232), and the gene expression profiles were painted onto a schematic of metabolic pathways. In doing so, those pathways that were significantly altered at each time point were identified.


As a result of this analysis the following observations were made: (1) Early in the cultures, genes encoding enzymes of the glycolytic pathway were slightly induced reflecting the change from partially depleted glucose levels in the inoculum culture to ample glucose in the shake flask medium; (2) As glucose was progressively depleted in the shake flask culture (times 6 and 8 hours), genes encoding glycolytic pathway enzymes were down-regulated; (3) genes that encode enzymes for phenylalanine, tryptophan, histidine, and arginine biosynthetic pathways were up-regulated during stationary phase suggesting that cells had exhausted critical amino acids, and they were turning on the genes for pathways to synthesize more of these essential nutrients; (4) as cells entered stationary phase, cell division, DNA replication, and ribosome production all decreased to a low level. This was reflected in the shut-down of purine and pyrimidine biosynthetic pathways and down-regulation of genes encoding many ribosomal proteins.


The invention described and claimed herein is not to be limited in scope by the specific aspects herein disclosed, since these aspects are intended as illustrations of several aspects of the invention. Any equivalent aspects are intended to be within the scope of this invention. Indeed, various modifications of the invention in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims. In the case of conflict, the present disclosure including definitions will control.


Various references are cited herein, the disclosures of which are incorporated by reference in their entireties.

Claims
  • 1. An isolated polypeptide having biological activity, selected from the group consisting of: (a) a polypeptide having biological activity comprising an amino acid sequence having at least 90% identity with an amino acid sequence selected from the group consisting of SEQ ID NOs: 4199-8395; and(b) a polypeptide having biological activity encoded by a gene comprising a nucleotide sequence which hybridizes under medium-high stringency conditions with a nucleotide sequence selected from the group consisting of SEQ ID NOs: 2-4198, or the full-length complementary strand thereof, wherein high stringency conditions are defined as prehybridization and hybridization at 42′C in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 35% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS 60° C.
  • 2. The polypeptide having biological activity of claim 1, which comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 4199-8395, or a fragment thereof which retains biological activity.
  • 3. The polypeptide having biological activity of claim 1, which consists of an amino acid sequence selected from the group consisting of SEQ ID NOs: 4199-8395, or a fragment thereof which retains biological activity.
  • 4. The polypeptide having biological activity of claim 1, which is encoded by a Bacillus licheniformis ATCC 14580 gene.
  • 5. The polypeptide having biological activity of claim 1, which comprises an amino acid sequence having at least 90% identity with an amino acid sequence selected from the group consisting of SEQ ID NOs: 4199-8395.
  • 6. The polypeptide having biological activity of claim 5, which comprises an amino acid sequence having at least 95% identity with an amino acid sequence selected from the group consisting of SEQ ID NOs: 4199-8395.
  • 7. The polypeptide having biological activity of claim 6, which comprises an amino acid sequence having at least 97% identity with an amino acid sequence selected from the group consisting of SEQ ID NOs: 4199-8395.
  • 8. The polypeptide having biological activity of claim 1, which is encoded by a gene comprising a nucleotide sequence which hybridizes under medium-high stringency conditions with a nucleotide sequence selected from the group consisting of SEQ ID NOs: 2-4198, or the full-length complementary strand thereof, wherein medium-high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 35% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 60° C.
  • 9. The polypeptide having biological activity of claim 8, which is encoded by a gene comprising a nucleotide sequence which hybridizes under high stringency conditions with a nucleotide sequence selected from the group consisting of SEQ ID NOs: 2-4198, or the full-length complementary strand thereof, wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.
CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional of U.S. application Ser. No. 12/322,974, filed Feb. 9, 2009, which is a divisional of U.S. application Ser. No. 10/983,128, filed Nov. 5, 2004, now U.S. Pat. No. 7,494,798, which claims the benefit of U.S. Provisional Application No. 60/535,988, filed Jan. 9, 2004, U.S. Provisional Application No. 60/561,059, filed Apr. 8, 2004, and U.S. Provisional Application No. 60/572,403, filed May 18, 2004, which applications are incorporated herein by reference.

Provisional Applications (3)
Number Date Country
60535988 Jan 2004 US
60561059 Apr 2004 US
60572403 May 2004 US
Divisions (2)
Number Date Country
Parent 12322974 Feb 2009 US
Child 12972306 US
Parent 10983128 Nov 2004 US
Child 12322974 US