Bacillus licheniformis chromosome

REFERENCE TO A SEQUENCE LISTING

This application contains a Sequence Listing in computer readable form, which is incorporated herein by reference.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to an isolated polynucleotide molecule comprising the complete chromosome of Bacillus licheniformis. The present invention also relates to features (genes) of the complete chromosomal DNA molecule of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the features as well as methods for producing biologically active substances encoded by the features and to methods of using the isolated features derived from the complete chromosomal DNA molecule of Bacillus licheniformis.

2. Description of the Related Art

Microbes, which make up most of the earth's biomass, have evolved for some 3.8 billion years. They are found in virtually every environment, surviving and thriving in extremes of heat, cold, radiation, pressure, salt, acidity, and darkness. Often in these environments, no other forms of life are found and the only nutrients come from inorganic matter. The diversity and range of their environmental adaptations indicate that microbes long ago “solved” many problems for which scientists are still actively seeking solutions. The value in determining the complete genome sequence of microbes is that it provides a detailed blueprint for the organism revealing all of the biochemical pathways, substrates, intermediates, and end products as well as regulatory networks, and evolutionary relationships to other microbes. A complete manifest of proteins, both structural and catalytic, is encoded as a list of features in the DNA molecule comprising the genome, as well as their likely cellular location.

Knowledge about the enormous range of microbial capacities has broad and far-reaching implications for environmental, energy, health, and industrial applications, such as cleanup of toxic-waste, production of novel therapeutic and preventive agents (drugs and vaccines), energy generation and development of renewable energy sources, production of chemical catalysts, reagents, and enzymes to improve efficiency of industrial processes, management of environmental carbon, nitrogen and nutrient cycling, detection of disease-causing organisms and monitoring of the safety of food and water supplies, use of genetically altered bacteria as living sensors (biosensors) to detect harmful chemicals in soil, air, or water, and understanding of specialized systems used by microbial cells to live in natural environments.

Bacillus licheniformis is a gram positive spore-forming bacterium that is widely distributed as a saprophytic organism in the environment. Unlike most other bacilli that are predominantly aerobic, Bacillus licheniformis is a facultative anaerobe which may allow it to grow in additional ecological niches. This species produces a diverse assortment of extracellular enzymes that are believed to contribute to the process of nutrient cycling in nature (Claus, D. and Berkeley, R. C. W., 1986, In Bergey's Manual of Systematic Bacteriology, Vol. 2., eds. Sneath, P. H. A. et al., Williams and Wilkins Co., Baltimore, Md., pp. 1105-1139). Certain Bacillus licheniformis isolates are capable of denitrification, however, the relevance of this characteristic to environmental denitrification may be small since the species generally persists in soil as endospores (Alexander, M., 1977, Introduction to Soil Microbiology. John Wiley and Sons, Inc., New York).

There are numerous industrial and agricultural uses for Bacillus licheniformis and its extracellular products. The species has been used for decades in the manufacture of industrial enzymes including several proteases, α-amylase, penicillinase, pentosanase, cycloglucosyltransferase, β-mannanase, and several pectinolytic enzymes, owing largely to its ability to secrete sizeable amounts of degradative enzymes. Bacillus licheniformis is also used to produce peptide antibiotics such as bacitracin and proticin, in addition to a number of specialty chemicals such as citric acid, inosine, inosinic acid, and poly-γ-glutamic acid. The proteases from Bacillus licheniformis are used in the detergent industry as well as for dehairing and batting of leather (Eveleigh, D. E., 1981, Scientific American 245, 155-178). Amylases from Bacillus licheniformis are deployed for the hydrolysis of starch, desizing of textiles, and sizing of paper (Erickson, R. J., 1976, In Microbiology, ed. Schlesinger, D. (Am. Soc. Microbiol., Washington, D.C.), pp. 406-419). Certain strains of Bacillus licheniformis have shown efficacy to destroy fungal pathogens affecting maize, grasses, and vegetable crops (U.S. Pat. No. 5,589,381; U.S. Pat. No. 5,665,354). As an endospore-forming bacterium, the ability of the organism to survive under unfavorable environmental conditions may enhance its potential as a natural control agent.

Bacillus licheniformis can be differentiated from other bacilli on the basis of metabolic and physiological tests (Logan, N. A. and Berkeley, R. C. W., 1981, In The Aerobic Endospore-Forming Bacteria Classification and Identification, eds. Berkeley, R. C. W. and Goodfellow, M., Academic Press, Inc., London, pp. 106-140; O'Donnell, A. G., Norris, J. R., Berkeley, R. C. W., Claus, D., Kanero, T., Logan, N. A., and Nozaki, R., 1980, Internat. J. Systematic Bacteriol. 30: 448-459). However, biochemical and phenotypic characteristics may be ambiguous among closely related species. Lapidus et al. (Lapidus, A., Galleron, N., Andersen, J. T., Jørgensen, P. L. Ehrlich, S. D., and Sorokin, A., 2002, FEMS Microbiol. Lett. 209: 23-30) recently constructed a physical map of the Bacillus licheniformis chromosome using a PCR approach, and established a number of regions of co-linearity where gene content and organization were ostensibly conserved with the Bacillus subtilis chromosome.

It would be advantageous to the art to have available the complete primary structure of the chromosomal DNA molecule of the Bacillus licheniformis type strain ATCC 14580. With the complete chromosome data in hand, it should be possible to do comparative genomics and proteomics studies that can lead to improved industrial strains as well as to a better understanding of genome evolution among closely-related bacilli in the subtilis-licheniformis group.

It is an object of the present invention to provide an isolated polynucleotide with the sequence of the complete chromosome of Bacillus licheniformis.

SUMMARY OF THE INVENTION

The present invention relates to an isolated polynucleotide of the complete chromosomal DNA molecule of Bacillus licheniformis ATCC 14580 having the nucleotide sequence of SEQ ID NO: 1.

The present invention also relates to isolated features (genes) of the complete chromosomal DNA molecule of Bacillus licheniformis ATCC 14580 encoding biologically active substances, selected from the group consisting of:

(a) a gene comprising a nucleotide sequence having at least 60% identity with any of the polynucleotides of SEQ ID NOs: 2-4198; and

(b) a gene comprising a nucleotide sequence which hybridizes under at least medium stringency conditions with any of the polynucleotides of SEQ ID NOs: 2-4198, or a complementary strand thereof.

The present invention also relates to biologically active substances encoded by the isolated genes, and nucleic acid constructs, vectors, and host cells comprising the genes.

The present invention also relates to methods for producing such substances having biological activity comprising (a) cultivating a recombinant host cell comprising a nucleic acid construct comprising a gene encoding the biologically active substance under conditions suitable for production of the biologically active substance; and (b) recovering the biologically active substance.

The present invention also relates to methods for monitoring differential expression of a plurality of genes in a first bacterial cell relative to expression of the same genes in one or more second bacterial cells, comprising:

(a) adding a mixture of detection reporter-labeled nucleic acids isolated from the bacterial cells to a substrate containing an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198, under conditions where the detection reporter-labeled nucleic acids hybridize to complementary sequences of the Bacillus licheniformis genes on the array, wherein the nucleic acids from the first bacterial cell and the one or more second bacterial cells are labeled with a first detection reporter and one or more different second detection reporters, respectively; and

(b) examining the array under conditions wherein the relative expression of the genes in the bacterial cells is determined by the observed detection signal of each spot on the array in which (i) the Bacillus licheniformis genes on the array that hybridize to the nucleic acids obtained from either the first or the one or more second bacterial cells produce a distinct first detection signal or one or more second detection signals, respectively, and (ii) the Bacillus licheniformis genes on the array that hybridize to the nucleic acids obtained from both the first and one or more second bacterial produce a distinct combined detection signal.

The present invention also relates to methods for isolating a gene encoding an enzyme, comprising:

(a) adding a mixture of labeled first nucleic acid probes, isolated from a microbial strain cultured on medium without an inducing substrate, and labeled second nucleic acid probes, isolated from the microbial strain cultured on medium with the inducing substrate, to an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198, under conditions where the labeled nucleic acid probes hybridize to complementary sequences of the Bacillus licheniformis genes on the array, wherein the first nucleic acid probes are labeled with a first reporter and the second nucleic acid probes are labeled with a second reporter;

(b) examining the array under conditions wherein the relative expression of the genes of the microbial strain is determined by the observed hybridization reporter signal of each spot on the array in which (i) the Bacillus licheniformis genes on the array that hybridize to the first nucleic acid probes produce a distinct first hybridization reporter signal or the second nucleic acid probes produce a distinct second hybridization reporter signal, and (ii) the Bacillus licheniformis genes on the array that hybridize to both the first and second nucleic acid probes produce a distinct combined hybridization reporter signal; and

The present invention also relates to genes isolated by such methods and nucleic acid constructs, vectors, and host cells containing the genes.

DEFINITIONS

Biologically active substance: The term “biologically active substance” is defined herein as any substance which is encoded by a single gene or a series of genes (contiguous or non-contiguous) composing a biosynthetic or metabolic pathway or operon or may be the direct or indirect result of the product of a single gene or products of a series of genes of the Bacillus licheniformis chromosome. Such substances include, but are not limited to, biopolymers, metabolites, and cellular structures and components (e.g., ribosome, flagella, etc.). For purposes of the present invention, biological activity is determined according to procedures known in the art such as those described by Carpenter and Sabatini, 2004, Nature 5: 11-22; Sordie et al., 2003, Proceedings of the National Academy of Sciences USA 100: 11964-11969; Braun and LaBaer, 2003, TRENDS in Biotechnology 21: 383-388; and Kaberdin and McDowall, 2003, Genome Research 13: 1961-1965.

In the methods of the present invention, the biopolymer may be any biopolymer. The term “biopolymer” is defined herein as a chain (or polymer) of identical, similar, or dissimilar subunits (monomers). The biopolymer may be, but is not limited to, a nucleic acid, polyamine, polyol, polypeptide (or polyamide), or polysaccharide.

In a preferred aspect, the biopolymer is a polypeptide. The polypeptide may be any polypeptide having a biological activity of interest. The term “polypeptide” is not meant herein to refer to a specific length of the encoded product and, therefore, encompasses peptides, oligopeptides, and proteins. The term “polypeptide” also encompasses naturally occurring allelic variations.

In a preferred aspect, the polypeptide is an antibody, antigen, antimicrobial peptide, enzyme, growth factor, hormone, immunodilator, neurotransmitter, receptor, reporter protein, structural protein, transcription factor, and transporter.

In a more preferred aspect, the polypeptide is an oxidoreductase, transferase, hydrolase, lyase, isomerase, or ligase. In a most preferred aspect, the polypeptide is an alpha-glucosidase, aminopeptidase, amylase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, esterase, alpha-galactosidase, beta-galactosidase, glucoamylase, glucocerebrosidase, alpha-glucosidase, beta-glucosidase, invertase, laccase, lipase, mannosidase, mutanase, oxidase, pectinolytic enzyme, peroxidase, phospholipase, phytase, polyphenoloxidase, proteolytic enzyme, ribonuclease, transglutaminase, urokinase, xylanase.

In another preferred aspect, the polypeptide is a collagen or gelatin.

In another preferred aspect, the biopolymer is a polysaccharide. The polysaccharide may be any polysaccharide, including, but not limited to, a mucopolysaccharide (e.g., heparin and hyaluronic acid) and a nitrogen-containing polysaccharide (e.g., chitin). In a more preferred aspect, the polysaccharide is hyaluronic acid (hyaluronan).

In the methods of the present invention, the metabolite may be any metabolite. The metabolite may be encoded by one or more genes, such as a biosynthetic or metabolic pathway. The term “metabolite” encompasses both primary and secondary metabolites. Primary metabolites are products of primary or general metabolism of a cell, which are concerned with energy metabolism, growth, and structure. Secondary metabolites are products of secondary metabolism (see, for example, R. B. Herbert, The Biosynthesis of Secondary Metabolites, Chapman and Hall, New York, 1981).

The primary metabolite may be, but is not limited to, an amino acid, fatty acid, nucleoside, nucleotide, sugar, triglyceride, vitamin.

The secondary metabolite may be, but is not limited to, an alkaloid, coumarin, flavonoid, polyketide, quinine, steroid, peptide, or terpene. In a preferred aspect, the secondary metabolite is an antibiotic, antifeedant, attractant, bacteriocide, fungicide, hormone, insecticide, or rodenticide.

Isolated biologically active substance: The term “isolated biologically active substance” is defined herein as a substance which is at least about 20% pure, preferably at least about 40% pure, more preferably about 60% pure, even more preferably about 80% pure, most preferably about 90% pure, and even most preferably about 95% pure, as determined by SDS-PAGE, HPLC, capillary electrophoresis, or any other method used in the art.

Substantially pure biologically active substance or pure biologically active substance: The term “substantially pure biologically active substance” is defined herein as a biologically active substance preparation which contains at most 10%, preferably at most 8%, more preferably at most 6%, more preferably at most 5% by weight, more preferably at most 4%, at most 3%, even more preferably at most 2%, most preferably at most 1%, and even most preferably at most 0.5% by weight of other material with which it is natively associated. It is, therefore, preferred that the substantially pure biologically active substance is at least 92% pure, preferably at least 94% pure, more preferably at least 95% pure, more preferably at least 96% pure, more preferably at least 96% pure, more preferably at least 97% pure, even more preferably at least 98% pure, most preferably at least 99%, and even most preferably at least 99.5% pure by weight of the total material present in the preparation. The term “pure biologically active substance” is defined as a biologically active substance preparation which contains no other material with which it is natively associated.

The biologically active substances of the present invention are preferably in a substantially pure form. In particular, it is preferred that the biologically active substances are in “essentially pure form”, i.e., that the biologically active substance preparation is essentially free of other material with which it is natively associated. This can be accomplished, for example, by preparing the biologically active substance by means of well-known recombinant methods or by classical purification methods.

Identity: The relatedness between two amino acid sequences or between two nucleotide sequences is described by the parameter “identity”.

For purposes of the present invention, the degree of identity between two amino acid sequences is determined by the Smith-Waterman Protein method for the Genematcher2, as implemented by Paracel Inc. (Pasadena, Calif.), or the BLASTP method as described by Altschul et al., 1990, Journal of Molecular Biology 215: 403-410.

For purposes of the present invention, the degree of identity between two nucleotide sequences is determined by the Smith Waterman nucleotide method for the Genematcher2 or BLASTN for the BlastMachine as implemented by Paracel Inc.

Polypeptide Fragment: The term “polypeptide fragment” is defined herein as a polypeptide, which retains biological activity, having one or more amino acids deleted from the amino and/or carboxyl terminus of a polypeptide encoded by any of the genes of the present invention, i.e., polypeptides of SEQ ID NOs: 4199-8395. Preferably, a fragment contains at least 80%, preferably at least 85%, more preferably at least 90%, even more preferably at least 95%, and most preferably at least 97% of the amino acid residues of the mature encoded polypeptide product.

Subsequence: The term “subsequence” is defined herein as a polynucleotide comprising a nucleotide sequence of any of SEQ ID NOs: 2-4198 except that one or more nucleotides have been deleted from the 5′ and/or 3′ end. Preferably, a subsequence contains at least 80%, preferably at least 85%, more preferably at least 90%, even more preferably at least 95%, and most preferably at least 97% of the nucleotides of any of the genes of the present invention.

Allelic variant: The term “allelic variant” denotes herein any of two or more alternative forms of a gene occupying the same chromosomal locus. Allelic variation arises naturally through mutation, and may result in polymorphism within populations. Gene mutations can be silent (no change in the encoded polypeptide) or may encode polypeptides having altered amino acid sequences. An allelic variant of a polypeptide is a polypeptide encoded by an allelic variant of a gene.

Substantially pure polynucleotide or pure polynucleotide: The term “substantially pure polynucleotide” as used herein refers to a polynucleotide preparation free of other extraneous or unwanted nucleotides and is in a form suitable for use within genetically engineered production systems. Thus, a substantially pure polynucleotide contains at most 10%, preferably at most 8%, more preferably at most 6%, more preferably at most 5%, more preferably at most 4%, more preferably at most 3%, even more preferably at most 2%, most preferably at most 1%, and even most preferably at most 0.5% by weight of other polynucleotide material with which it is natively associated. A substantially pure polynucleotide may, however, include naturally occurring 5′ and 3′ untranslated regions, such as promoters and terminators. It is preferred that the substantially pure polynucleotide is at least 92% pure, preferably at least 94% pure, more preferably at least 95% pure, moire preferably at least 96% pure, more preferably at least 96% pure, more preferably at least 97% pure, more preferably at least 98% pure, most preferably at least 99%, and even most preferably at least 99.5% pure by weight. The polynucleotides of the present invention are preferably in a substantially pure form. In particular, it is preferred that the polynucleotides disclosed herein are in “essentially pure form”, i.e., that the polynucleotide preparation is essentially free of other polynucleotide material with which it is natively associated. The term “pure polynucleotide” is defined as a polynucleotide preparation which contains no other material with which it is natively associated.

Nucleic acid construct: The term “nucleic acid construct” as used herein refers to a nucleic acid molecule, either single- or double-stranded, which is isolated from a naturally occurring gene or which has been modified to contain segments of nucleic acids in a manner that would not otherwise exist in nature. The term nucleic acid construct is synonymous with the term “expression cassette” when the nucleic acid construct contains the control sequences required for expression of a coding sequence of the present invention.

Control sequence: The term “control sequences” is defined herein to include all components, which are necessary or advantageous for the expression of a biologically active substance of the present invention. Each control sequence may be native or foreign to the polynucleotide encoding the substance. Such control sequences include, but are not limited to, a leader, propeptide sequence, promoter, signal peptide sequence, and transcription terminator. At a minimum, the control sequences include a promoter, and transcriptional and translational stop signals. The control sequences may be provided with linkers for the purpose of introducing specific restriction sites facilitating ligation of the control sequences with the coding region of the polynucleotide encoding a biologically active substance.

Operably linked: The term “operably linked” as used herein refers to a configuration in which a control sequence is placed at an appropriate position relative to the coding sequence of the DNA sequence, such that the control sequence directs the expression of a biologically active substance.

Coding sequence: When used herein the term “coding sequence” is intended to cover a nucleotide sequence, which directly specifies the amino acid sequence of its protein product. The boundaries of the coding sequence are generally determined by an open reading frame, which usually begins with the ATG start codon or alternative start codons such as GTG and TTG.

Expression: The term “expression” includes any step involved in the production of a biologically active substance including, but not limited to, transcription, post-transcriptional modification, translation, post-translational modification, and secretion.

Expression vector: The term “expression vector” herein covers a DNA molecule, linear or circular, that comprises a segment encoding a biologically active substance of the invention, and which is operably linked to additional segments that provide for its transcription.

Host cell: The term “host cell”, as used herein, includes any cell type which is susceptible to transformation, transfection, conjugation, electroporation, etc. with a nucleic acid construct, plasmid, or vector.

DETAILED DESCRIPTION OF THE INVENTION

Bacillus licheniformis Chromosome and Features (Genes) Thereof

The present invention relates to an isolated polynucleotide of the complete chromosomal DNA molecule of Bacillus licheniformis ATCC 14580 having the nucleotide sequence of SEQ ID NO: 1. Bacillus licheniformis ATCC 14580, consists of a circular molecule of 4,222,336 base pairs with a mean G+C content of 46.2%. The chromosome contains 4208 predicted protein-coding genes (SEQ ID NOs: 2-4198) with an average size of 873 bp, 7 rRNA operons, and 72 tRNA genes. The deduced amino acid sequences of the 4208 predicted protein-coding genes are shown in SEQ ID NOs: 4199-8395. SEQ ID NO: 4210 corresponds to SEQ ID NO: 2, SEQ ID NO: 4211 corresponds to SEQ ID NO: 3, SEQ ID NO: 4212 corresponds to SEQ ID NO: 4, etc. The predicted functions of the 4208 gene products are shown in Table 1.

The Bacillus licheniformis chromosome possesses regions that are markedly co-linear with the chromosomes of Bacillus subtilis and Bacillus halodurans, and approximately 80% of the predicted genes have Bacillus subtilis orthologues.

(a) a gene comprising a nucleotide sequence having at least 60% identity with any of the nucleotide sequences of SEQ ID NOs: 2-4198; and

(b) a gene comprising a nucleotide sequence which hybridizes under at least medium stringency conditions with any of the genes of SEQ ID NOs: 2-4198, or a complementary strand thereof.

In a first aspect, the present invention relates to isolated genes, which have a degree of identity to the nucleotide sequences of any of SEQ ID NOs: 2-4198 of at least about 60%, preferably at least about 65%, more preferably at least about 70%, more preferably at least about 75%, more preferably at least about 80%, more preferably at least about 85%, even more preferably at least about 90%, most preferably at least about 95%, and even most preferably at least about 97%, which encode biologically active substances having a particular biological activity (hereinafter “homologous biologically active substances”).

In a second aspect, the present invention relates to isolated genes comprising nucleotide sequences which hybridize under very low stringency conditions, preferably low stringency conditions, more preferably medium stringency conditions, more preferably medium-high stringency conditions, even more preferably high stringency conditions, and most preferably very high stringency conditions with any of (i) the genes of SEQ ID NOs: 2-4198, or subsequences thereof, or (ii) complementary strands thereof (J. Sambrook, E. F. Fritsch, and T. Maniatus, 1989, Molecular Cloning, A Laboratory Manual, 2d edition, Cold Spring Harbor, New York). Subsequences of SEQ ID NOs: 2-4198 may be at least 100 nucleotides or preferably at least 200 nucleotides. Moreover, the subsequences may encode fragments of a gene product which have biological activity. The biologically active substances may also be biologically active allelic variants of the biologically active substances.

The nucleotide sequences of SEQ ID NOs: 2-4198 or subsequences thereof, as well as the amino acid sequences of SEQ ID NOs: 4199-8395 or fragments thereof, may be used to design nucleic acid probes to identify and clone DNA encoding biologically active substances from strains of different genera or species according to methods well known in the art. In particular, such probes can be used for hybridization with the genomic DNA of the genus or species of interest, following standard Southern blotting procedures, in order to identify and isolate the corresponding gene therein. Such probes can be considerably shorter than the entire sequence, but should be at least 14, preferably at least 25, more preferably at least 35 nucleotides in length, such as at least 70 nucleotides in length. It is preferred, however, that the nucleic acid probes are at least 100 nucleotides in length. For example, the nucleic acid probes may be at least 200 nucleotides, at least 300 nucleotides, at least 400 nucleotides, or at least 500 nucleotides in length. Even longer probes may be used, e.g., nucleic acid probes which are at least 600 nucleotides, at least 700 nucleotides, at least 800 nucleotides, or at least 900 nucleotides in length. Both DNA and RNA probes can be used. The probes are typically labeled for detecting the corresponding gene (for example, with ³²P, ³H, ³⁵S, biotin, or avidin). Such probes are encompassed by the present invention.

A genomic DNA library prepared from such other organisms may, therefore, be screened for DNA which hybridizes with the probes described above and which encodes a biologically active substance. Genomic DNA from such other organisms may be separated by agarose or polyacrylamide gel electrophoresis, or other separation techniques. DNA from the libraries or the separated DNA may be transferred to and immobilized on nitrocellulose or other suitable carrier material. In order to identify a clone or DNA which is homologous with any of SEQ ID NOs: 2-4198 or subsequences thereof, the carrier material is used in a Southern blot.

For purposes of the present invention, hybridization indicates that a polynucleotide hybridizes to a labeled gene having the nucleotide sequence shown in any of SEQ ID NOs: 2-4198, complementary strands thereof, or subsequences thereof, under very low to very high stringency conditions. Molecules to which the nucleic acid probe hybridizes under these conditions can be detected using X-ray film.

In a preferred aspect, the nucleic acid probe is any of the genes of SEQ ID NOs: 2-4198, or subsequences thereof. In another preferred aspect, the nucleic acid probe is the mature coding region of any of the genes of SEQ ID NOs: 2-4198. In another preferred aspect, the nucleic acid probe is the gene of any of SEQ ID NOs: 2-4198 contained in Bacillus licheniformis ATCC 14580. In another preferred aspect, the nucleic acid probe is the mature coding region of any of the genes of SEQ ID NOs: 2-4198 contained in Bacillus licheniformis ATCC 14580.

For long probes of at least 100 nucleotides in length, very low to very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and either 25% formamide for very low and low stringencies, 35% formamide for medium and medium-high stringencies, or 50% formamide for high and very high stringencies, following standard Southern blotting procedures.

For long probes of at least 100 nucleotides in length, the carrier material is finally washed three times each for 15 minutes using 2×SSC, 0.2% SDS preferably at least at 45° C. (very low stringency), more preferably at least at 50° C. (low stringency), more preferably at least at 55° C. (medium stringency), more preferably at least at 60° C. (medium-high stringency), even more preferably at least at 65° C. (high stringency), and most preferably at least at 70° C. (very high stringency).

For short probes which are about 14 nucleotides to about 70 nucleotides in length, stringency conditions are defined as prehybridization, hybridization, and washing post-hybridization at about 5° C. to about 10° C. below the calculated T_musing the calculation according to Bolton and McCarthy (1962, Proceedings of the National Academy of Sciences USA 48:1390) in 0.9 M NaCl, 0.09 M Tris-HCl pH 7.6, 6 mM EDTA, 0.5% NP-40, 1×Denhardt's solution, 1 mM sodium pyrophosphate, 1 mM sodium monobasic phosphate, 0.1 mM ATP, and 0.2 mg of yeast RNA per ml following standard Southern blotting procedures.

For short probes which are about 14 nucleotides to about 70 nucleotides in length, the carrier material is washed once in 6×SCC plus 0.1% SDS for 15 minutes and twice each for 15 minutes using 6×SSC at 5′C to 10° C. below the calculated T_m.

Under salt-containing hybridization conditions, the effective T_mis what controls the degree of identity required between the probe and the filter bound DNA for successful hybridization. The effective T_mmay be determined using the formula below to determine the degree of identity required for two DNAs to hybridize under various stringency conditions.

Effective T_m=81.5+16.6(log M[Na⁺])+0.41(% G+C)−0.72(% formamide)

The % G+C content of any of the genes of SEQ ID NOs: 2-4198 can easily be determined. For medium stringency, for example, the concentration of formamide is 35% and the Na⁺ concentration for 5×SSPE is 0.75 M. Applying this formula to these values, the Effective T_min ° C. can be calculated. Another relevant relationship is that a 1% mismatch of two DNAs lowers the T_m1.4° C. To determine the degree of identity required for two DNAs to hybridize under medium stringency conditions at 42° C., the following formula is used:

% Homology=100−[(Effective T_m−Hybridization Temperature)/1.41]

Applying this formula, the degree of identity required for two DNAs to hybridize under medium stringency conditions at 42° C. can be calculated.

Similar calculations can be made under other stringency conditions, as defined herein.

The present invention also relates to isolated polynucleotides obtained by (a) hybridizing a population of DNA under very low, low, medium, medium-high, high, or very high stringency conditions with any of (i) the genes of SEQ ID NOs: 2-4198, or subsequences thereof, or (ii) complementary strands thereof; and (b) isolating the hybridizing polynucleotide from the population of DNA. In a preferred aspect, the hybridizing polynucleotide encodes a polypeptide of any of SEQ ID NOs: 2-4198, or homologous polypeptides thereof.

In a third aspect, the present invention relates to isolated polypeptides having amino acid sequences which have a degree of identity to any of SEQ ID NOs: 4199-8395 of at least about 60%, preferably at least about 65%, more preferably at least about 70%, more preferably at least about 75%, more preferably at least about 80%, more preferably at least about 85%, even more preferably at least about 90%, most preferably at least about 95%, and even most preferably at least about 97%, which have biological activity (hereinafter “homologous polypeptides”). In a preferred aspect, the homologous polypeptides have an amino acid sequence which differs by ten amino acids, preferably by five amino acids, more preferably by four amino acids, even more preferably by three amino acids, most preferably by two amino acids, and even most preferably by one amino acid from the amino acid sequences of SEQ ID NOs: 4199-8395.

The polypeptides of the present invention preferably comprise the amino acid sequence of any of SEQ ID NOs: 4199-8395 or an allelic variant thereof; or a fragment thereof that has biological activity. In a more preferred aspect, the polypeptides of the present invention comprise the amino acid sequence of any of SEQ ID NOs: 4199-8395. In another preferred aspect, the polypeptides of the present invention comprise the mature polypeptide region of any of SEQ ID NOs: 4199-8395, or an allelic variant thereof; or a fragment thereof that has biological activity. In another preferred aspect, the polypeptides of the present invention comprise the mature polypeptide region of any of SEQ ID NOs: 4199-8395. In another preferred aspect, the polypeptides of the present invention consist of the amino acid sequence of any of SEQ ID NOs: 4199-8395 or an allelic variant thereof; or a fragment thereof that has biological activity. In another preferred aspect, the polypeptides of the present invention consist of the amino acid sequence of any of SEQ ID NOs: 4199-8395. In another preferred aspect, the polypeptides consist of the mature polypeptide region of any of SEQ ID NOs: 4199-8395 or an allelic variant thereof; or a fragment thereof that has biological activity. In another preferred aspect, the polypeptides consist of the mature polypeptide region of any of SEC) ID NOs: 4199-8395.

In a fourth aspect, the present invention relates to isolated substances having biological activity which are encoded by polynucleotides which hybridize, as described above, under very low stringency conditions, preferably low stringency conditions, more preferably medium stringency conditions, more preferably medium-high stringency conditions, even more preferably high stringency conditions, and most preferably very high stringency conditions with which hybridize under very low stringency conditions, preferably low stringency conditions, more preferably medium stringency conditions, more preferably medium-high stringency conditions, even more preferably high stringency conditions, and most preferably very high stringency conditions with any of (i) the genes of SEQ ID NOs: 2-4198, or subsequences thereof, or (ii) complementary strands thereof. A subsequence of any of SEQ ID NOs: 2-4198 may be at least 100 nucleotides or preferably at least 200 nucleotides. Moreover, the subsequence may encode a fragment, e.g., a polypeptide fragment, which has biological activity.

Nucleic Acid Constructs

The present invention also relates to nucleic acid constructs comprising an isolated gene or isolated genes (e.g., operon) of the present invention operably linked to one or more control sequences which direct the expression of the coding sequence in a suitable host cell under conditions compatible with the control sequences.

An isolated gene(s) of the present invention may be manipulated in a variety of ways to provide for production of a biologically active substance encoded directly or indirectly by the gene(s). Manipulation of the nucleotide sequence prior to its insertion into a vector may be desirable or necessary depending on the expression vector. The techniques for modifying nucleotide sequences utilizing recombinant DNA methods are well known in the art.

The control sequence may be an appropriate promoter sequence, a nucleotide sequence which is recognized by a host cell for expression of the gene(s) encoding the biologically active substance. The promoter sequence contains transcriptional control sequences which mediate the expression of the biologically active substance. The promoter may be any nucleotide sequence which shows transcriptional activity in the host cell of choice including mutant, truncated, and hybrid promoters, and may be obtained from genes encoding extracellular or intracellular polypeptides or biologically active substances either homologous or heterologous to the host cell.

Examples of suitable promoters for directing the transcription of the nucleic acid constructs of the present invention, especially in a bacterial host cell, are the promoters obtained from the E. coli lac operon, Streptomyces coelicolor agarase gene (dagA), Bacillus subtilis levansucrase gene (sacB), Bacillus licheniformis alpha-amylase gene (amyL), Bacillus stearothermophilus maltogenic amylase gene (amyM), Bacillus amyloliquefaciens alpha-amylase gene (amyQ), Bacillus licheniformis penicillinase gene (penP), Bacillus subtilis xylA and xylB genes, and prokaryotic beta-lactamase gene (Villa-Kamaroff et al., 1978, Proceedings of the National Academy of Sciences USA 75: 3727-3731), as well as the tac promoter (DeBoer et al., 1983, Proceedings of the National Academy of Sciences USA 80: 21-25). Further promoters are described in “Useful proteins from recombinant bacteria” in Scientific American, 1980, 242: 74-94; and in Sambrook et al., 1989, supra.

The control sequence may also be a suitable transcription terminator sequence, a sequence recognized by a host cell to terminate transcription. The terminator sequence is operably linked to the 3′ terminus of the gene encoding the biologically active substance. Any terminator which is functional in the host cell of choice may be used in the present invention.

The control sequence may also be a signal peptide coding region that codes for an amino acid sequence linked to the amino terminus of a polypeptide and directs the encoded polypeptide into the cell's secretory pathway. The 5′ end of the coding sequence of the nucleotide sequence may inherently contain a signal peptide coding region naturally linked in translation reading frame with the segment of the coding region which encodes the secreted polypeptide. Alternatively, the 5′ end of the coding sequence may contain a signal peptide coding region which is foreign to the coding sequence. The foreign signal peptide coding region may be required where the coding sequence does not naturally contain a signal peptide coding region. Alternatively, the foreign signal peptide coding region may simply replace the natural signal peptide coding region in order to enhance secretion of the polypeptide. However, any signal peptide coding region which directs the expressed polypeptide into the secretory pathway of a host cell of choice may be used in the present invention.

Effective signal peptide coding regions for bacterial host cells are the signal peptide coding regions obtained from the genes for Bacillus NCIB 11837 maltogenic amylase, Bacillus stearothermophilus alpha-amylase, Bacillus licheniformis subtilisin, Bacillus licheniformis beta-lactamase, Bacillus stearothermophilus neutral proteases (nprT, nprS, nprM), and Bacillus subtilis prsA. Further signal peptides are described by Simonen and Palva, 1993, Microbiological Reviews 57: 109-137.

The control sequence may also be a propeptide coding region that codes for an amino acid sequence positioned at the amino terminus of a polypeptide. The resultant polypeptide is known as a proenzyme or propolypeptide (or a zymogen in some cases). A propolypeptide is generally inactive and can be converted to a mature active polypeptide by catalytic or autocatalytic cleavage of the propeptide from the propolypeptide. The propeptide coding region may be obtained from the genes for Bacillus subtilis alkaline protease (aprE) and Bacillus subtilis neutral protease (nprT).

Where both signal peptide and propeptide regions are present at the amino terminus of a polypeptide, the propeptide region is positioned next to the amino terminus of a polypeptide and the signal peptide region is positioned next to the amino terminus of the propeptide region.

It may also be desirable to add regulatory sequences which allow the regulation of the expression of a biologically active substance relative to the growth of the host cell. Examples of regulatory systems are those which cause the expression of the gene to be turned on or off in response to a chemical or physical stimulus, including the presence of a regulatory compound. Regulatory systems in prokaryotic systems include the lac, tac, and trp operator systems. Other examples of regulatory sequences are those which allow for gene amplification. In eukaryotic systems, these include the dihydrofolate reductase gene which is amplified in the presence of methotrexate, and the metallothionein genes which are amplified with heavy metals. In these cases, the nucleotide sequence encoding the biologically active substance would be operably linked with the regulatory sequence.

Expression Vectors

The present invention also relates to recombinant expression vectors comprising an isolated gene of the present invention, a promoter, and transcriptional and translational stop signals. The various nucleic acid and control sequences described above may be joined together to produce a recombinant expression vector which may include one or more convenient restriction sites to allow for insertion or substitution of the nucleotide sequence encoding the polypeptide at such sites. Alternatively, a gene of the present invention may be expressed by inserting the nucleotide sequence or a nucleic acid construct comprising the sequence into an appropriate vector for expression. In creating the expression vector, the coding sequence is located in the vector so that the coding sequence is operably linked with the appropriate control sequences for expression.

The recombinant expression vector may be any vector (e.g., a plasmid or virus) which can be conveniently subjected to recombinant DNA procedures and can bring about the expression of a gene of the present invention. The choice of the vector will typically depend on the compatibility of the vector with the host cell into which the vector is to be introduced. The vectors may be linear or closed circular plasmids.

The vector may be an autonomously replicating vector, i.e., a vector which exists as an extrachromosomal entity, the replication of which is independent of chromosomal replication, e.g., a plasmid, an extrachromosomal element, a minichromosome, or an artificial chromosome. The vector may contain any means for assuring self-replication. Alternatively, the vector may be one which, when introduced into the host cell, is integrated into the genome and replicated together with the chromosome(s) into which it has been integrated. Furthermore, a single vector or plasmid or two or more vectors or plasmids which together contain the total DNA to be introduced into the genome of the host cell, or a transposon may be used.

The vectors of the present invention preferably contain one or more selectable markers which permit easy selection of transformed cells. A selectable marker is a gene the product of which provides for biocide or viral resistance, resistance to heavy metals, prototrophy to auxotrophs, and the like.

Examples of bacterial selectable markers are the dal genes from Bacillus subtilis or Bacillus licheniformis, or markers which confer antibiotic resistance such as ampicillin, kanamycin, chloramphenicol or tetracycline resistance.

The vectors of the present invention preferably contain an element(s) that permits integration of the vector into the host cell's genome or autonomous replication of the vector in the cell independent of the genome.

For integration into the host cell genome, the vector may rely on portions of the sequence of the gene or any other element of the vector for integration of the vector into the genome by homologous or nonhomologous recombination. Alternatively, the vector may contain additional nucleotide sequences for directing integration by homologous recombination into the genome of the host cell. The additional nucleotide sequences enable the vector to be integrated into the host cell genome at a precise location(s) in the chromosome(s). To increase the likelihood of integration at a precise location, the integrational elements should preferably contain a sufficient number of nucleotides, such as 100 to 10,000 base pairs, preferably 400 to 10,000 base pairs, and most preferably 800 to 10,000 base pairs, which are highly homologous with the corresponding target sequence to enhance the probability of homologous recombination. The integrational elements may be any sequence that is homologous with the target sequence in the genome of the host cell. Furthermore, the integrational elements may be non-encoding or encoding nucleotide sequences. On the other hand, the vector may be integrated into the genome of the host cell by non-homologous recombination.

For autonomous replication, the vector may further comprise an origin of replication enabling the vector to replicate autonomously in the host cell in question. The origin of replication may be any plasmid replicator mediating autonomous replication which functions in a cell. The term “origin of replication” or “plasmid replicator” is defined herein as a sequence that enables a plasmid or vector to replicate in vivo. Examples of bacterial origins of replication are the origins of replication of plasmids pBR322, pUC19, pACYC177, and pACYC184 permitting replication in E. coli, and pUB110, pE194, pTA1060, and pAMβ1 permitting replication in Bacillus.

More than one copy of a gene of the present invention may be inserted into the host cell to increase production of the gene product. An increase in the copy number of the gene can be obtained by integrating at least one additional copy of the sequence into the host cell genome or by including an amplifiable selectable marker gene with a gene of the present invention where cells containing amplified copies of the selectable marker gene, and thereby additional copies of the gene of the present invention, can be selected for by cultivating the cells in the presence of the appropriate selectable agent.

The procedures used to ligate the elements described above to construct the recombinant expression vectors of the present invention are well known to one skilled in the art (see, e.g., Sambrook et al., 1989, supra).

Host Cells

The present invention also relates to recombinant host cells, comprising an isolated gene of the present invention, where the host cells are advantageously used in the recombinant production of a biologically active substance encoded by the gene. A vector comprising a gene of the present invention is introduced into a host cell so that the vector is maintained as a chromosomal integrant or as a self-replicating extra-chromosomal vector as described earlier. The term “host cell” encompasses any progeny of a parent cell that is not identical to the parent cell due to mutations that occur during replication. The choice of a host cell will to a large extent depend upon the gene encoding the biologically active substance and its source.

The host cell may be any unicellular microorganism, e.g., a prokaryote.

Useful unicellular cells are bacterial cells such as gram positive bacteria including, but not limited to, a Bacillus cell, e.g., Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus cereus, Bacillus circulans, Bacillus clausii, Bacillus coagulans, Bacillus fastidiosus, Bacillus firmus, Bacillus lautus, Bacillus lentus, Bacillus licheniformis, Bacillus macerans, Bacillus megaterium, Bacillus methanolicus, Bacillus purnilus, Bacillus sphaericus, Bacillus stearothermophilus, Bacillus subtilis, and Bacillus thuringiensis; or a Streptomyces cell, e.g., Streptomyces lividans and Streptomyces murinus, or gram negative bacteria such as E. coli and Pseudomonas sp. In a preferred aspect, the bacterial host cell is a Bacillus lentus, Bacillus licheniformis, Bacillus stearothermophilus, or Bacillus subtilis cell. In another preferred aspect, the Bacillus cell is an alkalophilic Bacillus.

The introduction of a vector into a bacterial host cell may, for instance, be effected by protoplast transformation (see, e.g., Chang and Cohen, 1979, Molecular General Genetics 168: 111-115), using competent cells (see, e.g., Young and Spizizen, 1961, Journal of Bacteriology 81: 823-829, or Dubnau and Davidoff-Abelson, 1971, Journal of Molecular Biology 56: 209-221), electroporation (see, e.g., Shigekawa and Dower, 1988, Biotechniques 6: 742-751), or conjugation (see, e.g., Koehler and Thorne, 1987, Journal of Bacteriology 169: 5771-5278).

Methods of Production

The present invention also relates to methods for producing a biologically active substance of the present invention comprising (a) cultivating a strain, which in its wild-type form is capable of producing the biologically active substance, under conditions conducive for production of the biologically active substance; and (b) recovering the biologically active substance. Preferably, the strain is of the genus Bacillus, and more preferably Bacillus licheniformis.

The present invention also relates to methods for producing a biologically active substance of the present invention comprising (a) cultivating a host cell under conditions conducive for production of the biologically active substance, wherein the host cell comprises a mutant polynucleotide comprising at least one mutation in the coding region of any of SEQ ID NOs: 2-4198, wherein the mutant polynucleotide encodes a biologically active substance which consists of SEQ ID NOs: 4199-8395, respectively, and (b) recovering the biologically active substance.

In the production methods of the present invention, the cells are cultivated in a nutrient medium suitable for production of the biologically active substance using methods known in the art. For example, the cell may be cultivated by shake flask cultivation, and small-scale or large-scale fermentation (including continuous, batch, fed-batch, or solid state fermentations) in laboratory or industrial fermentors performed in a suitable medium and under conditions allowing the biologically active substance to be expressed and/or isolated. The cultivation takes place in a suitable nutrient medium comprising carbon and nitrogen sources and inorganic salts, using procedures known in the art. Suitable media are available from commercial suppliers or may be prepared according to published compositions (e.g., in catalogues of the American Type Culture Collection). If the biologically active substance is secreted into the nutrient medium, the biologically active substance can be recovered directly from the medium. If the biologically active substance is not secreted, it can be recovered from cell lysates.

The biologically active substances may be detected using methods known in the art that are specific for the polypeptides. These detection methods may include use of specific antibodies, formation of an enzyme product, or disappearance of an enzyme substrate. For example, an enzyme assay may be used to determine the activity of an enzyme.

The resulting biologically active substances may be recovered by methods known in the art. For example, the biologically active substances may be recovered from the nutrient medium by conventional procedures including, but not limited to, centrifugation, filtration, extraction, spray-drying, evaporation, or precipitation.

The biologically active substances of the present invention may be purified by a variety of procedures known in the art including, but not limited to, chromatography (e.g., ion exchange, affinity, hydrophobic, chromatofocusing, and size exclusion), electrophoretic procedures (e.g., preparative isoelectric focusing), differential solubility (e.g., ammonium sulfate precipitation), SDS-PAGE, or extraction (see, e.g., Protein Purification, J.-C. Janson and Lars Ryden, editors, VCH Publishers, New York, 1989).

Plants

The present invention also relates to a transgenic plant, plant part, or plant cell which has been transformed with a gene encoding a biologically active substance of the present invention so as to express and produce the biologically active substance in recoverable quantities. The biologically active substance may be recovered from the plant or plant part. Alternatively, the plant or plant part containing the recombinant biologically active substance may be used as such for improving the quality of a food or feed, e.g., improving nutritional value, palatability, and rheological properties, or to destroy an antinutritive factor.

The transgenic plant can be dicotyledonous (a dicot) or monocotyledonous (a monocot). Examples of monocot plants are grasses, such as meadow grass (blue grass, Poa), forage grass such as festuca, lolium, temperate grass, such as Agrostis, and cereals, e.g., wheat, oats, rye, barley, rice, sorghum, and maize (corn).

Examples of dicot plants are tobacco, legumes, such as lupins, potato, sugar beet, pea, bean and soybean, and cruciferous plants (family Brassicaceae), such as cauliflower, rape seed, and the closely related model organism Arabidopsis thaliana.

Examples of plant parts are stem, callus, leaves, root, fruits, seeds, and tubers as well as the individual tissues comprising these parts, e.g., epidermis, mesophyll, parenchyma, vascular tissues, meristems. In the present context, also specific plant cell compartments, such as chloroplast, apoplast, mitochondria, vacuole, peroxisomes and cytoplasm are considered to be a plant part. Furthermore, any plant cell, whatever the tissue origin, is considered to be a plant part. Likewise, plant parts such as specific tissues and cells isolated to facilitate the utilisation of the invention are also considered plant parts e.g. embryos, endosperms, aleurone and seeds coats.

Also included within the scope of the present invention are the progeny of such plants, plant parts, and plant cells.

The transgenic plant or plant cell expressing a biologically active substance of the present invention may be constructed in accordance with methods known in the art. Briefly, the plant or plant cell is constructed by incorporating one or more expression constructs encoding a biologically active substance of the present invention into the plant host genome and propagating the resulting modified plant or plant cell into a transgenic plant or plant cell.

The expression construct is conveniently a nucleic acid construct which comprises a gene encoding a biologically active substance of the present invention operably linked with appropriate regulatory sequences required for expression of the nucleotide sequence in the plant or plant part of choice. Furthermore, the expression construct may comprise a selectable marker useful for identifying host cells into which the expression construct has been integrated and DNA sequences necessary for introduction of the construct into the plant in question (the latter depends on the DNA introduction method to be used).

The choice of regulatory sequences, such as promoter and terminator sequences and optionally signal or transit sequences is determined, for example, on the basis of when, where, and how the biologically active substance is desired to be expressed. For instance, the expression of the gene encoding a biologically active substance of the present invention may be constitutive or inducible, or may be developmental, stage or tissue specific, and the gene product may be targeted to a specific tissue or plant part such as seeds or leaves. Regulatory sequences are, for example, described by Tague et al., 1988, Plant Physiology 86: 506.

For constitutive expression the 35S-CaMV, the maize ubiquitin 1 and the rice actin 1 promoter may be used (Franck et al., 1980. Cell 21: 285-294, Christensen A H, Sharrock R A and Quail, 1992, Plant Mo. Biol. 18: 675-689; Zhang W, McElroy D. and Wu R., 1991, Plant Cell 3: 1155-1165). Organ-specific promoters may be, for example, a promoter from storage sink tissues such as seeds, potato tubers, and fruits (Edwards and Coruzzi, 1990, Ann. Rev. Genet. 24: 275-303), or from metabolic sink tissues such as meristems (Ito et al., 1994, Plant Mol. Biol. 24: 863-878), a seed specific promoter such as the glutelin, prolamin, globulin, or albumin promoter from rice (Wu et al., 1998, Plant and Cell Physiology 39: 885-889), a Vicia faba promoter from the legumin B4 and the unknown seed protein gene from Vicia faba (Conrad et al., 1998, Journal of Plant Physiology 152: 708-711), a promoter from a seed oil body protein (Chen et al., 1998, Plant and Cell Physiology 39: 935-941), the storage protein napA promoter from Brassica napus, or any other seed specific promoter known in the art, e.g., as described in WO 91/14772. Furthermore, the promoter may be a leaf specific promoter such as the rbcs promoter from rice or tomato (Kyozuka et al., 1993, Plant Physiology 102: 991-1000, the chlorella virus adenine methyltransferase gene promoter (Mitra and Higgins, 1994, Plant Molecular Biology 26: 85-93), or the aldP gene promoter from rice (Kagaya et al., 1995, Molecular and General Genetics 248: 668-674), or a wound inducible promoter such as the potato pint promoter (Xu et al., 1993, Plant Molecular Biology 22: 573-588). Likewise, the promoter may inducible by abiotic treatments such as temperature, drought or alterations in salinity or induced by exogenously applied substances that activate the promoter, e.g., ethanol, oestrogens, plant hormones like ethylene, abscisic acid and gibberellic acid and heavy metals.

A promoter enhancer element may also be used to achieve higher expression of the enzyme in the plant. For instance, the promoter enhancer element may be an intron which is placed between the promoter and the nucleotide sequence encoding a biologically active substance of the present invention. For instance, Xu et al., 1993, supra disclose the use of the first intron of the rice actin 1 gene to enhance expression.

The selectable marker gene and any other parts of the expression construct may be chosen from those available in the art.

The nucleic acid construct is incorporated into the plant genome according to conventional techniques known in the art, including Agrobacterium-mediated transformation, virus-mediated transformation, microinjection, particle bombardment, biolistic transformation, and electroporation (Gasser et al., 1990, Science 244: 1293; Potrykus, 1990, Bio/Technology 8; 535: Shimamoto et al., 1989, Nature 338: 274).

Presently, Agrobacterium tumefaciens-mediated gene transfer is the method of choice for generating transgenic dicots (for a review, see Hooykas and Schilperoort, 1992, Plant Molecular Biology 19: 15-38). However it can also be used for transforming monocots, although other transformation methods are generally preferred for these plants. Presently, the method of choice for generating transgenic monocots is particle bombardment (microscopic gold or tungsten particles coated with the transforming DNA) of embryonic calli or developing embryos (Christou, 1992, Plant Journal 2: 275-281; Shimamoto, 1994, Current Opinion Biotechnology 5: 158-162; Vasil et al., 1992, Bio/Technology 10: 667-674). An alternative method for transformation of monocots is based on protoplast transformation as described by Omirulleh et al., 1993, Plant Molecular Biology 21: 415-428.

Following transformation, the transformants having incorporated therein the expression construct are selected and regenerated into whole plants according to methods well-known in the art. Often the transformation procedure is designed for the selective elimination of selection genes either during regeneration or in the following generations by using, for example, co-transformation with two separate T-DNA constructs or site specific excision of the selection gene by a specific recombinase.

The present invention also relates to methods for producing a biologically active substance of the present invention comprising (a) cultivating a transgenic plant or a plant cell comprising a gene encoding a biologically active substance of the present invention under conditions conducive for production of the biologically active substance; and (b) recovering the biologically active substance.

Removal or Reduction of Biologically Active Substance

The present invention also relates to methods for producing a mutant of a parent cell, which comprises disrupting or deleting all or a portion of a gene encoding a biologically active substance of the present invention, which results in the mutant cell producing less of the biologically active substance than the parent cell when cultivated under the same conditions.

The mutant cell may be constructed by reducing or eliminating expression of a gene encoding or regulatory synthesis of a biologically active substance of the present invention using methods well known in the art, for example, insertions, disruptions, replacements, or deletions. The gene to be modified or inactivated may be, for example, the coding region or a part thereof essential for activity, or a regulatory element of the gene required for the expression of the coding region. An example of such a regulatory or control sequence may be a promoter sequence or a functional part thereof, i.e., a part that is sufficient for affecting expression of the gene. Other control sequences for possible modification include, but are not limited to, a leader, propeptide sequence, signal peptide sequence, transcription terminator, and transcriptional activator.

Modification or inactivation of the gene may be performed by subjecting the parent cell to mutagenesis and selecting for mutant cells in which expression of the gene has been reduced or eliminated. The mutagenesis, which may be specific or random, may be performed, for example, by use of a suitable physical or chemical mutagenizing agent, by use of a suitable oligonucleotide, or by subjecting the DNA sequence to PCR generated mutagenesis. Furthermore, the mutagenesis may be performed by use of any combination of these mutagenizing agents.

Examples of a physical or chemical mutagenizing agent suitable for the present purpose include ultraviolet (UV) irradiation, hydroxylamine, N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), O-methyl hydroxylamine, nitrous acid, ethyl methane sulphonate (EMS), sodium bisulphite, formic acid, and nucleotide analogues.

When such agents are used, the mutagenesis is typically performed by incubating the parent cell to be mutagenized in the presence of the mutagenizing agent of choice under suitable conditions, and screening and/or selecting for mutant cells exhibiting reduced or no expression of the gene.

Modification or inactivation of the nucleotide sequence may be accomplished by introduction, substitution, or removal of one or more nucleotides in the gene or a regulatory element required for the transcription or translation thereof. For example, nucleotides may be inserted or removed so as to result in the introduction of a stop codon, the removal of the start codon, or a change in the open reading frame. Such modification or inactivation may be accomplished by site-directed mutagenesis or PCR generated mutagenesis in accordance with methods known in the art. Although, in principle, the modification may be performed in vivo, i.e., directly on the cell expressing the nucleotide sequence to be modified, it is preferred that the modification be performed in vitro as exemplified below.

An example of a convenient way to eliminate or reduce expression of a nucleotide sequence by a cell of choice is based on techniques of gene replacement, gene deletion, or gene disruption. For example, in the gene disruption method, a nucleic acid sequence corresponding to the endogenous nucleotide sequence is mutagenized in vitro to produce a defective nucleic acid sequence which is then transformed into the parent cell to produce a defective gene. By homologous recombination, the defective nucleic acid sequence replaces the endogenous nucleotide sequence. It may be desirable that the defective nucleotide sequence also encodes a marker that may be used for selection of transformants in which the nucleotide sequence has been modified or destroyed. In a particularly preferred aspect, the nucleotide sequence is disrupted with a selectable marker such as those described herein.

Alternatively, modification or inactivation of the nucleotide sequence may be performed by established anti-sense techniques using a sequence complementary to the nucleotide sequence. More specifically, expression of the nucleotide sequence by a cell may be reduced or eliminated by introducing a sequence complementary to the nucleic acid sequence of the gene that may be transcribed in the cell and is capable of hybridizing to the mRNA produced in the cell. Under conditions allowing the complementary anti-sense nucleotide sequence to hybridize to the mRNA, the amount of protein translated is thus reduced or eliminated.

The present invention further relates to a mutant cell of a parent cell which comprises a disruption or deletion of a nucleotide sequence encoding the biologically active substance or a control sequence thereof, which results in the mutant cell producing less of the biologically active substance than the parent cell.

The biologically active substance-deficient mutant cells so created are particularly useful as host cells for the expression of homologous and/or heterologous substances, such as polypeptides. Therefore, the present invention further relates to methods for producing a homologous or heterologous substance comprising (a) cultivating the mutant cell under conditions conducive for production of the substance; and (b) recovering the substance. The term “heterologous substances” is defined herein as substances which are not native to the host cell, a native substance in which modifications have been made to alter the native sequence, or a native substance whose expression is quantitatively altered as a result of a manipulation of the host cell by recombinant DNA techniques.

In a further aspect, the present invention relates to a method for producing a protein product essentially free of a biologically active substance by fermentation of a cell which produces both a biologically active substance of the present invention as well as the protein product of interest by adding an effective amount of an agent capable of inhibiting activity of the biologically active substance to the fermentation broth before, during, or after the fermentation has been completed, recovering the product of interest from the fermentation broth, and optionally subjecting the recovered product to further purification.

In accordance with this aspect of the invention, it is possible to remove at least 60%, preferably at least 75%, more preferably at least 85%, still more preferably at least 95%, and most preferably at least 99% of the biologically active substance. Complete removal of biologically active substance may be obtained by use of this method.

The methods used for cultivation and purification of the product of interest may be performed by methods known in the art.

The methods of the present invention for producing an essentially biologically active substance-free product is of particular interest in the production of prokaryotic polypeptides, in particular bacterial proteins such as enzymes. The enzyme may be selected from, e.g., an amylolytic enzyme, lipolytic enzyme, proteolytic enzyme, cellulytic enzyme, oxidoreductase, or plant cell-wall degrading enzyme. Examples of such enzymes include an aminopeptidase, amylase, amyloglucosidase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, esterase, galactosidase, beta-galactosidase, glucoamylase, glucose oxidase, glucosidase, haloperoxidase, hemicellulase, invertase, isomerase, laccase, ligase, lipase, lyase, mannosidase, oxidase, pectinolytic enzyme, peroxidase, phytase, phenoloxidase, polyphenoloxidase, proteolytic enzyme, ribonuclease, transferase, transglutaminase, or xylanase. The biologically active substance-deficient cells may also be used to express heterologous proteins of pharmaceutical interest such as hormones, growth factors, receptors, and the like.

It will be understood that the term “prokaryotic polypeptides” includes not only native polypeptides, but also those polypeptides, e.g., enzymes, which have been modified by amino acid substitutions, deletions or additions, or other such modifications to enhance activity, thermostability, pH tolerance and the like.

In a further aspect, the present invention relates to a product of a protein or substance essentially free of a biologically active substance of the invention, produced by a method of the present invention.

Compositions

The present invention also relates to compositions comprising a biologically active substance of the present invention. Preferably, the compositions are enriched in the biologically active substance. The term “enriched” indicates that the biologically active substance of the composition has been increased, e.g., with an enrichment factor of 1.1.

The composition may comprise a biologically active substance of the invention as the major component, e.g., a mono-component composition. Alternatively, the composition may comprise multiple biologically active substances, for example, multiple enzymes, such as an aminopeptidase, amylase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, esterase, alpha-galactosidase, beta-galactosidase, glucoamylase, alpha-glucosidase, beta-glucosidase, haloperoxidase, invertase, laccase, lipase, mannosidase, oxidase, pectinolytic enzyme, peptidoglutaminase, peroxidase, phytase, polyphenoloxidase, proteolytic enzyme, ribonuclease, transglutaminase, or xylanase.

The compositions may be prepared in accordance with methods known in the art and may be in the form of a liquid or a dry composition. For instance, the composition may be in the form of a granulate or a microgranulate. The biologically active substance to be included in the composition may be stabilized in accordance with methods known in the art.

Methods for Using the Bacillus licheniformis Chromosome

The present invention also relates to methods for using the Bacillus licheniformis chromosome.

The chromosome of Bacillus licheniformis serves as a reservoir of new genes/proteins that have likely environmental, energy, health, and industrial applications (e.g., enzymes, antibiotics, biochemicals). A clear extension of this is that the newly discovered molecules can be used as starting points for further improvements via well-established gene shuffling, directed evolution, and protein engineering methods. Additionally, regions or motifs (e.g., signal peptides, active sites, substrate-binding regions) from the newly discovered molecules may be employed to derive novel chimeras with industrially advantageous properties.

The genes encoded in the chromosome may be used for monitoring global gene expression during the life cycle of the organism or during industrial fermentations (e.g., implemented on DNA microarrays). By monitoring global gene expression, for example, improved processes for industrial fermentation can be implemented with greater efficiency and economy.

The chromosome is useful in comparative evolutionary and ecological studies. For example, dozens of Bacillus licheniformis isolates can be readily compared on a global scale by hybridization of their genomic DNAs to a microarray fabricated from the reference strain presented in this case (so-called comparative genomic hybridization). Using this method, one can compare various isolates to look for similarities/differences among geographical and environmental niches or among biocontrol strains versus saprophytic isolates.

The chromosome sequence may be used to construct the metabolic blueprint for Bacillus licheniformis that includes all catabolic and anabolic pathways, signaling pathways, regulatory networks, growth substrates, biochemical intermediates, end products, electron donors/acceptors and others. In doing so, it is possible to modify the metabolic machinery of the organism by deleting unwanted pathways and/or adding enzymes/pathways from other organisms to generate useful chemicals and intermediates.

The pathways and components that contribute to production of extracellular and surface proteins in Bacillus licheniformis can be extracted from the chromosomal sequence. This affords opportunities for improved production of extracellular proteins by genetic manipulation of the secretion machinery.

The chromosome data allows deduction of the essential genes for Bacillus licheniformis (either by comparison to related bacteria such as Bacillus subtilis or by systematic gene-by-gene knock outs). Thus it has become possible to design custom-made strains which contain only the genes that are essential for production of specific proteins or metabolites (so-called cell factory concept).

The chromosome data may be used to construct interspecies hybrids between Bacillus licheniformis and other bacteria. Venter et al., 2003, Proc. Nat. Acad. Sci. USA 100, 15440-15445 have shown that it is possible to construct an entire virus genome from smaller DNA segments. Thus, segments of the Bacillus licheniformis chromosome may be employed to derive novel chromosomal segments or even entire chimeric chromosomes for specific applications.

In a preferred aspect, methods for using the Bacillus licheniformis chromosome include host improvement, e.g., secretion of a protein or metabolite, genome shuffling, construction of new genomes, metabolic engineering and pathway reconstruction, carrier for heterologous expression vectors, microarrays as described herein, identification of polypeptides in proteomics analyses, and comparative genomics with other Bacillus species or related organisms.

Methods for Isolating Genes

The present invention also relates to methods for isolating a gene encoding a biologically active substance from a microbial strain. The method comprises first the addition of a mixture of first labeled nucleic acid probes, isolated from a microbial strain cultured on medium without an inducing substrate, and a mixture of second labeled nucleic acid probes, isolated from the microbial strain cultured on medium with the inducing substrate, to an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198, under conditions where the labeled nucleic acid probes hybridize to complementary sequences of the Bacillus licheniformis genes on the array. The first nucleic acid probes are labeled with a first reporter and the second nucleic acid probes are labeled with a second reporter. The array is then examined under conditions wherein the relative expression of the genes of the microbial strain is determined by the observed hybridization reporter signal of each spot on the array in which (i) the Bacillus licheniformis genes on the array that hybridize to the first nucleic acid probes produce a distinct first hybridization reporter signal or to the second nucleic acid probes produce a distinct second hybridization reporter signal, and (ii) the Bacillus licheniformis genes on the array that hybridize to both the first and second nucleic acid probes produce a distinct combined hybridization reporter signal. The probe is then sequenced to isolate from the microbial strain the corresponding gene that encodes an enzyme that degrades or converts the substrate.

Enzymes. The gene of interest may encode any enzyme including an oxidoreductase, transferase, hydrolase, lyase, isomerase, or ligase. In a preferred aspect, the enzyme is an acylase, alpha-glucosidase, amidase, aminopeptidase, amylase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase, cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, dextrinase, endoglucanase, esterase, galactanase, alpha-galactosidase, beta-galactosidase, glucoamylase, glucanase, glucocerebrosidase, alpha-glucosidase, beta-glucosidase, hemicellulase, invertase, laccase, lignase, lipase, lysin, mannosidase, mutanase, oxidase, pectinolytic enzyme, peroxidase, phosphatase, phospholipase, phytase, polyphenoloxidase, proteolytic enzyme, pullulanase, ribonuclease, transglutaminase, urokinase, or xylanase.

Inducing Substrate. The inducing substrate may be any substrate that is subject to the action of an enzyme, i.e., that degrades or converts the substrate. In a preferred aspect, the inducing substrate is lignin or a lignin-containing material. In a more preferred aspect, the lignin-containing material is lignocellulose. In another preferred aspect, the inducing substrate is cellulose. In another preferred aspect, the inducing substrate is hemicellulose. In another preferred aspect, the inducing substrate is pectin. In another preferred aspect, the inducing substrate is a lipid. In another preferred aspect, the inducing substrate is phospholipid. In another preferred aspect, the inducing substrate is phytic acid. In another preferred aspect, the inducing substrate is protein. In another preferred aspect, the inducing substrate is a starch. In another preferred aspect, the inducing substrate is a medium that is low in nutrients such as amino acids, carbon, nitrogen, phosphate, or iron.

In a more preferred aspect, the protein substrate is blood, casein, egg, gelatin, gluten, milk protein, or soy protein. In another more preferred aspect, the lignin-containing material is hardwood thermomechanical pulp. In another more preferred aspect, the lignocellulose is corn stover. In another more preferred aspect, the lignocellulose is white poplar. In another more preferred aspect, the lignocellulose is rice straw. In another more preferred aspect, the lignocellulose is switch grass.

Microbial Strains. In the methods of the present invention, the microbial strain may be any microbial strain. The strain is cultured on a suitable nutrient medium with and without a substrate of interest. The strain cultured on medium without the substrate is used as a reference for identifying differences in expression of the same or similar complement of genes in the strain cultured on medium with substrate. The strain may be a wild-type, mutant, or recombinant strain.

In the methods of the present invention, the microbial strain is preferably a bacterium. In a more preferred aspect, the bacterium is a Bacillus, Pseudomonas, or Streptomyces strain or E. coli.

The Bacillus strain may be any Bacillus strain. In a preferred aspect, the Bacillus strain is Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus cereus, Bacillus circulans, Bacillus clausii, Bacillus coagulans, Bacillus fastidiosus, Bacillus firmus, Bacillus lautus, Bacillus lentos, Bacillus licheniformis, Bacillus macerans, Bacillus megaterium, Bacillus methanolicus, Bacillus pumilus, Bacillus sphaericus, Bacillus stearothermophilus, Bacillus subtilis, or Bacillus thuringiensis. It will be understood that the term “Bacillus” also encompasses relatives of Bacillus such as Paenibacillus, Oceanobacillus, and the like.

The Pseudomonas strain may be any Pseudomonas strain. In a preferred aspect, the Pseudomonas strain is Pseudomonas acidovorans, Pseudomonas aeruginosa, Pseudomonas alcaligenes, Pseudomonas anguilliseptica, Pseudomonas abtimicrobica, Pseudomonas aurantiaca, Pseudomonas aureofaciens, Pseudomonas beijerinckii, Pseudomonas boreopolis, Pseudomonas chlororaphis, Pseudomonas citronellolis, Pseudomonas cocovenenans, Pseudomonas diminuta, Pseudomonas doudoroffii, Pseudomonas echinoides, Pseudomonas elongata, Pseudomonas fluorescens, Pseudomonas fragi, Pseudomonas halophobica, Pseudomonas huttiensis, Pseudomonas indigofera, Pseudomonas lanceolata, Pseudomonas lemoignei, Pseudomonas lundensis, Pseudomonas mendocina, Pseudomonas mephitica, Pseudomonas mucidolens, Pseudomonas oleovorans, Pseudomonas phenazinium, Pseudomonas pictorium, Pseudomonas putida, Pseudomonas resinovorans, Pseudomonas saccharophila, Pseudomonas stanieri, Pseudomonas stutzeri, Pseudomonas taetrolens, or Pseudomonas vesicularis.

The Streptomyces strain may be any Streptomyces strain. In a preferred aspect, the Streptomyces strain is Streptomyces lividans. In another preferred aspect, the Streptomyces strain is Streptomyces murinus.

Microarrays. The term “an array of Bacillus licheniformis genes” is defined herein as a linear or two-dimensional array of preferably discrete elements of an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198 (e.g., synthetic oligonucleotides of, for example, 40-60 nucleotides), wherein each discrete element has a finite area, formed on the surface of a solid support. It shall be understood that the term “Bacillus licheniformis genes” encompasses nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198.

The term “microarray” is defined herein as an array of Bacillus licheniformis gene elements having a density of discrete of Bacillus licheniformis gene elements of at least about 100/cm², and preferably at least about 1000/cm². The Bacillus licheniformis gene elements in a microarray have typical dimensions, e.g., diameters, in the range of between about 10 to about 250 μm, preferably in the range of between about 10 to about 200 μm, more preferably in the range of between about 20 to about 150 μm, even more preferably in the range of between about 20 to about 100 μm, most preferably in the range of between about 50 to about 100 μm, and even most preferably in the range of between about 80 to about 100 μm, and are separated from other gene elements in the microarray by about the same distance.

Methods and instruments for forming microarrays on the surface of a solid support are well known in the art. See, for example, U.S. Pat. No. 5,807,522; U.S. Pat. No. 5,700,637; and U.S. Pat. No. 5,770,151. The instrument may be an automated device such as described in U.S. Pat. No. 5,807,522.

The term “a substrate containing an array of Bacillus licheniformis genes” is defined herein as a solid support having deposited on the surface of the support one or more of a plurality of Bacillus licheniformis genes, as described herein, for use in detecting binding of labeled nucleic acids to the Bacillus licheniformis genes.

The substrate may, in one aspect, be a glass support (e.g., glass slide) having a hydrophilic or hydrophobic coating on the surface of the support, and an array of distinct random nucleic acid fragments bound to the coating, where each distinct random nucleic acid fragment is disposed at a separate, defined position.

Each microarray in the substrate preferably contains at least 10³distinct Bacillus licheniformis in a surface area of less than about 5 or 6 cm². Each distinct Bacillus licheniformis gene (i) is disposed at a separate, defined position on the array, (ii) has a length of at least 50 bp, and (iii) is present in a defined amount between about 0.1 femtomoles and 100 nanomoles or higher if necessary.

For a hydrophilic coating, the glass slide is coated by placing a film of a polycationic polymer with a uniform thickness on the surface of the slide and drying the film to form a dried coating. The amount of polycationic polymer added should be sufficient to form at least a monolayer of polymers on the glass surface. The polymer film is bound to the surface via electrostatic binding between negative silyl-OH groups on the surface and charged cationic groups in the polymers. Such polycationic polymers include, but are not limited to, polylysine and polyarginine.

Another coating strategy employs reactive aldehydes to couple DNA to the slides (Schena et al., 1996, Proceedings of the National Academy of Science USA 93: 10614-10619; Heller at al., 1997, Proceedings of the National Academy of Science USA 94: 2150-2155).

Alternatively, the surface may have a relatively hydrophobic character, i.e., one that causes aqueous medium deposited on the surface to bead. A variety of known hydrophobic polymers, such as polystyrene, polypropylene, or polyethylene, have desirable hydrophobic properties, as do glass and a variety of lubricant or other hydrophobic films that may be applied to the support surface. A support surface is “hydrophobic” if an aqueous droplet applied to the surface does not spread out substantially beyond the area size of the applied droplet, wherein the surface acts to prevent spreading of the droplet applied to the surface by hydrophobic interaction with the droplet.

In another aspect, the substrate may be a multi-cell substrate where each cell contains a microarray of Bacillus licheniformis and preferably an identical microarray, formed on a porous surface. For example, a 96-cell array may typically have array dimensions between about 12 and 244 mm in width and 8 and 400 mm in length, with the cells in the array having width and length dimension of 1/12 and ⅛ the array width and length dimensions, respectively, i.e., between about 1 and 20 in width and 1 and 50 mm in length.

The solid support may include a water-impermeable backing such as a glass slide or rigid polymer sheet, or other non-porous material. Formed on the surface of the backing is a water-permeable film, which is formed of porous material. Such porous materials include, but are not limited to, nitrocellulose membrane nylon, polypropylene, and polyvinylidene difluoride (PVDF) polymer. The thickness of the film is preferably between about 10 and 1000 μm. The film may be applied to the backing by spraying or coating, or by applying a preformed membrane to the backing.

Alternatively, the solid support may be simply a filter composed of nitrocellulose, nylon, polypropylene, or polyvinylidene difluoride (PVDF) polymer, or, for that matter, any material suitable for use.

The film surface may be partitioned into a desirable array of cells by water-impermeable grid lines typically at a distance of about 100 to 2000 μm above the film surface. The grid lines can be formed on the surface of the film by laying down an uncured flowable resin or elastomer solution in an array grid, allowing the material to infiltrate the porous film down to the backing, and then curing the grid lines to form the cell-array substrate.

The barrier material of the grid lines may be a flowable silicone, wax-based material, thermoset material (e.g., epoxy), or any other useful material. The grid lines may be applied to the solid support using a narrow syringe, printing techniques, heat-seal stamping, or any other useful method known in the art.

Each well preferably contains a microarray of distinct Bacillus licheniformis genes. “Distinct Bacillus licheniformis genes” as applied to the genes forming a microarray is defined herein as an array member which is distinct from other array members on the basis of a different Bacillus licheniformis gene sequence or oligo sequence thereof, and/or different concentrations of the same or distinct Bacillus licheniformis genes and/or different mixtures of distinct Bacillus licheniformis genes or different-concentrations of Bacillus licheniformis genes. Thus an array of “distinct Bacillus licheniformis genes” may be an array containing, as its members, (i) distinct Bacillus licheniformis genes which may have a defined amount in each member, (ii) different, graded concentrations of a specific Bacillus licheniformis gene, and/or (iii) different-composition mixtures of two or more distinct Bacillus licheniformis genes.

It will be understood, however, that in the methods of the present invention, any type of substrate known in the art may be used.

The delivery of a known amount of a selected Bacillus licheniformis gene to a specific position on the support surface is preferably performed with a dispensing device equipped with one or more tips for insuring reproducible deposition and location of the Bacillus licheniformis genes and for preparing multiple arrays. Any dispensing device known in the art may be used in the methods of the present invention. See, for example, U.S. Pat. No. 5,807,522.

For liquid-dispensing on a hydrophilic surface, the liquid will have less of a tendency to bead, and the dispensed volume will be more sensitive to the total dwell time of the dispenser tip in the immediate vicinity of the support surface.

For liquid-dispensing on a hydrophobic surface, flow of fluid from the tip onto the support surface will continue from the dispenser onto the support surface until it forms a liquid bead. At a given bead size, i.e., volume, the tendency of liquid to flow onto the surface will be balanced by the hydrophobic surface interaction of the bead with the support surface, which acts to limit the total bead area on the surface, and by the surface tension of the droplet, which tends toward a given bead curvature. At this point, a given bead volume will have formed, and continued contact of the dispenser tip with the bead, as the dispenser tip is being withdrawn, will have little or no effect on bead volume.

The desired deposition volume, i.e., bead volume, formed is preferably in the range 2 pl (picoliters) to 2 nl (nanoliters), although volumes as high as 100 nl or more may be dispensed. It will be appreciated that the selected dispensed volume will depend on (i) the “footprint” of the dispenser tip(s), i.e., the size of the area spanned by the tip(s), (ii) the hydrophobicity of the support surface, and (iii) the time of contact with and rate of withdrawal of the tip(s) from the support surface. In addition, bead size may be reduced by increasing the viscosity of the medium, effectively reducing the flow time of liquid from the dispensing device onto the support surface. The drop size may be further constrained by depositing the drop in a hydrophilic region surrounded by a hydrophobic grid pattern on the support surface.

At a given tip size, bead volume can be reduced in a controlled fashion by increasing surface hydrophobicity, reducing time of contact of the tip with the surface, increasing rate of movement of the tip away from the surface, and/or increasing the viscosity of the medium. Once these parameters are fixed, a selected deposition volume in the desired picoliter to nanoliter range can be achieved in a repeatable fashion.

After depositing a liquid droplet of a Bacillus licheniformis gene sample at one selected location on a support, the tip may be moved to a corresponding position on a second support, the Bacillus licheniformis gene sample is deposited at that position, and this process is repeated until the random nucleic acid fragment sample has been deposited at a selected position on a plurality of supports.

This deposition process may then be repeated with another random nucleic acid fragment sample at another microarray position on each of the supports.

The diameter of each Bacillus licheniformis gene region is preferably between about 20-200 μm. The spacing between each region and its closest (non-diagonal) neighbor, measured from center-to-center, is preferably in the range of about 20-400 μm. Thus, for example, an array having a center-to-center spacing of about 250 μm contains about 40 regions/cm or 1,600 regions/cm². After formation of the array, the support is treated to evaporate the liquid of the droplet forming each region, to leave a desired array of dried, relatively flat Bacillus licheniformis gene or oligo thereof regions. This drying may be done by heating or under vacuum. The DNA can also be UV-crosslinked to the polymer coating.

Nucleic Add Probes. In the methods of the present invention, the strains are cultivated in a nutrient medium with and without a substrate using methods well known in the art for isolation of nucleic acids to be used as probes. For example, the strains may be cultivated by shake flask cultivation, small-scale or large-scale fermentation (including continuous, batch, fed-batch, or solid state fermentations) in laboratory or industrial fermentors performed in a suitable medium. The cultivation takes place in a suitable nutrient medium comprising carbon and nitrogen sources and inorganic salts, using procedures known in the art. Suitable media are available from commercial suppliers or may be prepared according to published compositions (e.g., in catalogues of the American Type Culture Collection).

The nucleic acid probes from the microbial strains cultured on medium with and without substrate may be any nucleic acid including genomic DNA, cDNA, and RNA, and may be isolated using standard methods known in the art.

The populations of isolated nucleic acid probes may be labeled with detection reporters such as colorimetric, radioactive for example, ³²P, ³³P, or ³⁵S), fluorescent reporters, or other reporters using methods known in the art (Chen et al., 1998, Genomics 51: 313-324; DeRisi at al., 1997, Science 278: 680-686; U.S. Pat. No. 5,770,367).

In a preferred aspect, the probes are labeled with fluorescent reporters. For example, the DNA probes may be labeled during reverse transcription from the respective RNA pools by incorporation of fluorophores as dye-labeled nucleotides (DeRisi et al., 1997, supra), e.g., Cy5-labeled deoxyuridine triphosphate, or the isolated cDNAs may be directly labeled with different fluorescent functional groups. Fluorescent-labeled nucleotides include, but are not limited to, fluorescein conjugated nucleotide analogs (green fluorescence), lissamine nucleotide analogs (red fluorescence). Fluorescent functional groups include, but are not limited to, Cy3 (a green fluorescent dye) and Cy5 (red fluorescent dye).

Array Hybridization. The labeled nucleic acids from the two strains cultivated with and without substrate are then added to an array of Bacillus licheniformis genes under conditions where the nucleic acid pools from the two strains hybridize to complementary sequences of the Bacillus licheniformis genes on the array. For purposes of the present invention, hybridization indicates that the labeled nucleic acids from the two strains hybridize to the Bacillus licheniformis genes under very low to very high stringency conditions.

A small volume of the labeled nucleic acids mixture is loaded onto the substrate. The solution will spread to cover the entire microarray. In the case of a multi-cell substrate, one or more solutions are loaded into each cell which stop at the barrier elements.

For nucleic add probes of at least about 100 nucleotides in length, microarray hybridization conditions described by Eisen and Brown, 1999, Methods of Enzymology 303: 179-205, may be used. Hybridization is conducted under a cover slip at 65° C. in 3×SSC for 4-16 hours followed by post-hybridization at room temperature after removal of the cover slip in 2×SSC, 0.1% SDS by washing the array two or three times in the solution, followed by successive washes in 1×SSC for 2 minutes and 0.2×SSC wash for two or more minutes.

Conventional conditions of very low to very high stringency conditions may also be used. Very low to very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and either 25% formamide for very low and low stringencies, 35% formamide for medium and medium-high stringencies, or 50% formamide for high and very high stringencies, following standard Southern blotting procedures.

The carrier material is finally washed three times each for 15 minutes using 2×SSC, 0.2% SDS preferably at least at 45° C. (very low stringency), more preferably at east at 50° C. (low stringency), more preferably at least at 55° C. (medium stringency), more preferably at least at 60° C. (medium-high stringency), even more preferably at least at 65° C. (high stringency), and most preferably at least at 70° C. (very high stringency).

For shorter nucleic acid probes which are less than 50 nucleotides, microarray hybridization conditions described by Kane et al., 2000, Nucleic Acids Research 28: 4552-4557, may be used. Hybridization is conducted under a supported coverslip at 42° C. for 16-18 hours at high humidity in 50% formamide, 4.1×Denhardt's solution, 4.4×SSC, and 100 μg/ml of herring sperm DNA. Arrays are washed after removal of the coverslip in 4×SSC by immersion into 1×SSC, 0.1% SDS for 10 minutes, 0.1×SSC, 0.1% SDS twice for 10 minutes, and 0.1×SSC twice for 10 minutes.

For shorter nucleic acid probes which are about 50 nucleotides to about 100 nucleotides in length, conventional stringency conditions may be used. Such stringency conditions are defined as prehybridization, hybridization, and washing post-hybridization at 5° C. to 10° C. below the calculated T_musing the calculation according to Bolton and McCarthy (1962, Proceedings of the National Academy of Sciences USA 48:1390) in 0.9 M NaCl, 0.09 M Tris-HCl pH 7.6, 6 mM EDTA, 0.5% NP-40, 1×Denhardt's solution, 1 mM sodium pyrophosphate, 1 mM sodium monobasic phosphate, 0.1 mM ATP, and 0.2 mg of yeast RNA per ml following standard Southern blotting procedures.

The carrier material is finally washed once in 6×SSC plus 0.1% SDS for 15 minutes and twice each for 15 minutes using 6×SSC at 5° C. to 10° C. below the calculated T_m.

The choice of hybridization conditions will depend on the degree of homology between the Bacillus licheniformis genes and the nucleic acid probes obtained from the strain cultured with and without inducing substrate. For example, where the nucleic acid probes and the Bacillus licheniformis genes are obtained from identical strains, high stringency conditions may be most suitable. Where the strains are from a genus or species different from which the Bacillus licheniformis genes were obtained, low or medium stringency conditions may be more suitable.

In a preferred aspect, the hybridization is conducted under low stringency conditions. In a more preferred aspect, the hybridization is conducted under medium stringency conditions. In a most preferred aspect, the hybridization is conducted under high stringency conditions.

The entire solid support is than reacted with detection reagents if needed and analyzed using standard colorimetric, radioactive, or fluorescent detection means. All processing and detection steps are performed simultaneously to all of the microarrays on the solid support ensuring uniform assay conditions for all of the microarrays on the solid support.

Detection. The most common detection method is laser-induced fluorescence detection using confocal optics (Cheung et al., 1998, Nat. Genet. 18: 225-230). The array is examined under fluorescence excitation conditions such that (i) the Bacillus licheniformis genes on the array that hybridize to the first nucleic acid probes obtained from the strain cultured without inducing substrate and to the second nucleic acid probes obtained from the strain cultured with inducing substrate produce a distinct first fluorescence emission color and a distinct second fluorescence emission color, respectively, and (ii) the Bacillus licheniformis genes on the array that hybridize to substantially equal numbers of nucleic acid probes obtained from the strain cultured without inducing substrate and from the strain cultured with inducing substrate produce a distinct combined fluorescence emission color; wherein the relative expression of the genes in the strains can be determined by the observed fluorescence emission color of each spot on the array.

The fluorescence excitation conditions are based on the selection of the fluorescence reporters. For example, Cy3 and Cy5 reporters are detected with solid state lasers operating at 532 nm and 632 nm, respectively.

However, other methods of detection well known in the art may be used such as standard photometric, calorimetric, or radioactive detection means, as described earlier.

Data Analysis. The data obtained from the scanned image may then be analyzed using any of the commercially available image analysis software. The software preferably identifies array elements, subtracts backgrounds, deconvolutes multi-color images, flags or removes artifacts, verifies that controls have performed properly, and normalizes the signals (Chen et al., 1997, Journal of Biomedical Optics 2: 364-374).

Several computational methods have been described for the analysis and interpretation of microarray-based expression profiles including cluster analysis (Eisen at al., 1998, Proc. Nat Acad. Sci. USA 95: 14863-14868), parametric ordering of genes (Spellman et al., 1998, Mol. Biol. Cell 9: 3273-3297), and supervised clustering methods based on representative hand-picked or computer-generated expression profiles (Chu at al., 1998. Science 282: 699-705). Preferred methods for evaluating the results of the microarrays employ statistical analysis to determine the significance of the differences in expression levels. In the methods of the present invention, the difference in the detected expression level is at least about 10% or greater, preferably at least about 20% or greater, more preferably at least about 50% or greater, even more preferably at least about 75% or greater; and most preferably at least about 100% or greater.

One such preferred system is the Significance Analysis of Microarrays (SAM) (Tusher et al., 2001, Proc. Natl. Acad. Sci. USA 98: 5116-5121). Statistical analysis allows the determination of significantly altered expression of levels of about 50% or even less. The PAM (or predictive analysis for microarrays) represents another approach for analyzing the results of the microarrays (Tibshirani at al., 2002, Proc. Natl. Acad. Sci. USA 99: 6567-6572).

Cluster algorithms may also be used to analyze microarray expression data. From the analysis of the expression profiles it is possible to identify co-regulated genes that perform common metabolic or biosynthetic functions. Hierarchical clustering has been employed in the analysis of microarray expression data in order to place genes into clusters based on sharing similar patterns of expression (Eisen at al., 1998, supra). This method yields a graphical display that resembles a kind of phylogenetic tree where the relatedness of the expression behavior of each gene to every other gene is depicted by branch lengths. The programs Cluster and TreeView, both written by Michael Eisen (Eisen et al., 1998 Proc. Nat. Acad. Sci. USA 95: 14863-14868) are freely available. Genespring is a commercial program available for such analysis (Silicon Genetics, Redwood City, Calif.).

Self-organizing maps (SOMs), a non-hierarchical method, have also been used to analyze microarray expression data (Tamayo et al., 1999, Proc. Natl. Acad. Sri. USA 96: 2907-2912). This method involves selecting a geometry of nodes, where the number of nodes defines the number of clusters. Then, the number of genes analyzed and the number of experimental conditions that were used to provide the expression values of these genes are subjected to an iterative process (20,000-50,000 iterations) that maps the nodes and data points into multidimensional gene expression space. After the identification of significantly regulated genes, the expression level of each gene is normalized across experiments. As a result, the expression profile of the genome is highlighted in a manner that is relatively independent of each gene's expression magnitude. Software for the “GENECLUSTER” SOM program for microarray expression analysis can be obtained from the Whitehead/MIT Center for Genome Research. SOMs can also be constructed using the GeneSpring software package.

Isolation of Genes. Probes containing genes or portions thereof identified to be induced by the present of substrate in the medium are characterized by determining the sequence of the probe. Based on the sequence, the gene can then be isolated using methods well known in the art.

The techniques used to isolate or clone a gene include isolation from genomic DNA, preparation from cDNA, or a combination thereof. The cloning of the gene from such genomic DNA can be effected, e.g., by using the well known polymerase chain reaction (PCR) or antibody screening of expression libraries to detect cloned DNA fragments with shared structural features. See, e.g., Innis at al., 1990, PCR: A Guide to Methods and Application, Academic Press, New York. Other nucleic acid amplification procedures such as ligase chain reaction (LCR), ligated activated transcription (LAT) and nucleic acid sequence-based amplification (NASBA) may be used. The gene may be cloned from the strain of interest, or another or related organism and thus, for example, may be an allelic or species variant of the gene.

Methods for Monitoring Differential Expression of a Plurality of Genes

(a) adding a mixture of detection reporter-labeled nucleic acids isolated from the bacterial cells to a substrate containing an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198, under conditions where the detection reporter-labeled nucleic acids hybridize to complementary sequences of the Bacillus licheniformis genes on the array, wherein the nucleic acids from the first bacterial cell and the one or more second bacterial cells are labeled with a first detection reporter and one or more different second detection reporters, respectively; and

The methods of the present invention may be used to monitor global expression of a plurality of genes from a Bacillus cell, discover new genes, identify possible functions of unknown open reading frames, and monitor gene copy number variation and stability. For example, the global view of changes in expression of genes may be used to provide a picture of the way in which Bacillus cells adapt to changes in culture conditions, environmental stress, or other physiological provocation. Other possibilities for monitoring global expression include spore morphogenesis, recombination, metabolic or catabolic pathway engineering.

The methods of the present invention are particularly advantageous since one spot on an array equals one gene or open reading frame because extensive follow-up characterization is unnecessary since sequence information is available, and the Bacillus licheniformis microarrays can be organized based on function of the gene products.

Microarrays. Methods for preparing the microarrays are described herein.

Bacterial Cells. In the methods of the present invention, the two or more Bacillus cells may be any Bacillus cell where one of the cells is used as a reference for identifying differences in expression of the same or similar complement of genes in the other cell(s). In one aspect, the two or more cells are the same cell. For example, they may be compared under different growth conditions, e.g., oxygen limitation, nutrition, and/or physiology. In another aspect, one or more cells are mutants of the reference cell. For example, the mutant(s) may have a different phenotype. In a further aspect, the two or more cells are of different species (e.g., Bacillus clausii and Bacillus subtilis). In another further aspect, the two or more cells are of different genera. In an even further aspect, one or more cells are transformants of the reference cell, wherein the one or more transformants exhibit a different property. For example, the transformants may have an improved phenotype relative to the reference cell and/or one of the other transformants. The term “phenotype” is defined herein as an observable or outward characteristic of a cell determined by its genotype and modulated by its environment. Such improved phenotypes may include, but are not limited to, improved secretion or production of a protein or compound, reduced or no secretion or production of a protein or compound, improved or reduced expression of a gene, desirable morphology, an altered growth rate under desired conditions, relief of over-expression mediated growth inhibition, or tolerance to low oxygen conditions.

The Bacillus cells may be any Bacillus cells, but preferably Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus cereus, Bacillus circulans, Bacillus clausii, Bacillus coagulans, Bacillus fastidiosus, Bacillus firmus, Bacillus lautus, Bacillus lentus, Bacillus licheniformis, Bacillus macerans, Bacillus megaterium, Bacillus methanolicus, Bacillus pumilus, Bacillus sphaericus, Bacillus stearothermophilus, Bacillus subtilis, or Bacillus thuringiensis cells.

In a preferred aspect, the Bacillus cells are Bacillus alkalophilus cells. In another preferred aspect. The Bacillus cells are Bacillus amyloliquefaciens cells. In another preferred aspect, the Bacillus cells are Bacillus brevis cells. In another preferred aspect, the Bacillus cells are Bacillus cereus cells. In another preferred aspect, the Bacillus cells are Bacillus circulans cells. In another preferred aspect, the Bacillus cells are Bacillus clausii cells. In another preferred aspect, the Bacillus cells are Bacillus coagulans cells. In another preferred aspect, the Bacillus cells are Bacillus fastidiosus cells. In another preferred aspect, the Bacillus cells are Bacillus firmus cells. In another preferred aspect, the Bacillus cells are Bacillus lautus cells. In another preferred aspect, the Bacillus cells are Bacillus lentus cells. In another preferred aspect, the Bacillus cells are Bacillus licheniformis cells. In another preferred aspect, the Bacillus cells are Bacillus macerans cells. In another preferred aspect, the Bacillus cells are Bacillus megaterium cells. In another preferred aspect, the Bacillus cells are Bacillus methanolicus cells. In another preferred aspect, the Bacillus cells are Bacillus pumilus cells. In another preferred aspect, the Bacillus cells are Bacillus sphaericus cells. In another preferred aspect, the Bacillus cells are Bacillus stearothermophilus cells. In another preferred aspect, the Bacillus cells are Bacillus subtilis cells. In another preferred aspect, the Bacillus cells are Bacillus thuringiensis cells.

In a more preferred aspect, the Bacillus cells are Bacillus licheniformis cells. In a most preferred aspect, the Bacillus licheniformis cells are Bacillus licheniformis ATCC 14580 cells.

In another more preferred aspect, the Bacillus cells are Bacillus clausii cells. In another most preferred aspect, the Bacillus clausii cells are Bacillus clausii NCIB 10309 cells.

It will be understood that the term “Bacillus” also encompasses relatives of Bacillus such as Paenibacillus, Oceanobacillus, and the like.

In the methods of the present invention, the cells are cultivated in a nutrient medium suitable for growth using methods well known in the art for isolation of the nucleic acids to be used as probes. For example, the cells may be cultivated by shake flask cultivation, small-scale or large-scale fermentation (including continuous, batch, fed-batch, or solid state fermentations) in laboratory or industrial fermentors performed in a suitable medium. The cultivation takes place in a suitable nutrient medium comprising carbon and nitrogen sources and inorganic salts, using procedures known in the art. Suitable media are available from commercial suppliers or may be prepared according to published compositions (e.g., in catalogues of the American Type Culture Collection).

Nucleic Acid Probes. The nucleic acid probes from the two or more Bacillus cells may be any nucleic acid including genomic DNA, cDNA, and RNA, and may be isolated using standard methods known in the art, as described herein. The populations of isolated nucleic acid probes may be labeled with colorimetric, radioactive, fluorescent reporters, or other reporters using methods described herein.

In a preferred aspect, the probes are labeled with fluorescent reporters, e.g., Cy3 (a green fluorescent dye) and Cy5 (red fluorescent dye), as described herein.

Array Hybridization. The labeled nucleic acids from the two or more Bacillus cells are then added to a substrate containing an array of Bacillus licheniformis genes under conditions, as described herein, where the nucleic acid pools from the two or more Bacillus cells hybridize to complementary sequences of the Bacillus licheniformis genes on the array.

Detection and Data Analysis. The same methods as described herein are used for detection and data analysis.

Computer Readable Media and Computer-Based Systems

The Bacillus licheniformis chromosome and its genes described herein may be “provided” in a variety of media to facilitate their use. The term “provided” refers to a manufacture comprising an array of Bacillus licheniformis genes. Such manufactures provide the Bacillus licheniformis genes in a form which allows one skilled in the art to examine the manufacture using means not directly applicable to examining the chromosome or a subset thereof as it exists in nature or in purified form.

Thus, the present invention also relates to such a manufacture in the form of a computer readable medium comprising an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198.

In one application of this aspect, the Bacillus licheniformis genes of the present invention can be recorded on computer readable media. The term “computer readable media” is defined herein as any medium which can be read and accessed by a computer. Such computer readable media include, but are not limited to, magnetic storage media, e.g., floppy discs, hard disc storage medium, and magnetic tape; optical storage media, e.g., CD-ROM, DVD; electrical storage media, e.g., RAM and ROM; and hybrids of these categories, e.g., magnetic/optical storage media. One skilled in the art can readily appreciate how any of the presently known computer readable media can be used to create a manufacture comprising computer readable medium having recorded thereon a nucleotide sequence of the present invention. Likewise, it will be clear to those of skill how additional computer readable media that may be developed also can be used to create analogous manufactures having recorded thereon a nucleotide sequence of the present invention.

As used herein, “recorded” refers to a process for storing information on computer readable medium. One skilled in the art can readily adopt any of the presently known methods for recording information on computer readable medium to generate manufactures comprising the nucleotide sequence information of the present invention.

A variety of data storage structures are available for creating a computer readable medium having recorded thereon a nucleotide sequence of the present invention. The choice of the data storage structure will generally be based on the means chosen to access the stored information. In addition, a variety of data processor programs and formats can be used to store the nucleotide sequence information of the present invention on computer readable medium. The sequence information can be represented in a word processing text file, formatted in commercially-available software such as WordPerfect and Microsoft Word, or represented in the form of an ASCII file, stored in a database application, such as DB2, Sybase, Oracle, or the like. A skilled artisan can readily adapt any number of data-processor structuring formats (e.g., text file or database) in order to obtain computer readable medium having recorded thereon the nucleotide sequence information of the present invention.

Various computer software programs are publicly available that allow a skilled artisan to access sequence information provided in a computer readable medium. Thus, by providing in computer readable form an array of Bacillus licheniformis genes selected from the group consisting of nucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs: 2-4198, enables one skilled in the art to routinely access the provided sequence information for a wide variety of purposes.

Software utilizing the BLAST (Altschul et al., 1990, supra), BLAZE (Brutlag et al., 1993, Comp. Chem. 17: 203-207), GENEMARK (Lukashin and Borodovsky, 1998, Nucleic Acids Research 26: 1107-1115), GENSCAN (Burge and Karlin, 1997, Journal of Molecular Biology 268: 78-94), GLIMMER (Salzberg et al., 1998, Nucleic Acids Research 26: 544-548), and GRAIL (Xu et al., 1994, Comput. Appl. Biosci. 10: 613-623) search algorithms may be used to identify open reading frames (ORFS) within a genome of interest, which contain homology to ORFs or proteins from both Bacillus licheniformis and Bacillus clausii and from other organisms. Among the ORFs discussed herein are protein encoding fragments of the Bacillus licheniformis and Bacillus clausii genomes useful in producing commercially important proteins, such as enzymes used in fermentation reactions and in the production of commercially useful metabolites.

The present invention further provides systems, particularly computer-based systems, which contain the sequence information described herein. Such systems are designed to identify, among other things, genes and gene products—many of which could be products themselves or used to genetically modify an industrial expression host through increased or decreased expression of a specific gene sequence(s).

The term “a computer-based system” is herein defined as the hardware means, software means, and data storage means used to analyze the nucleotide sequence information of the present invention. The minimum hardware means of the computer-based systems of the present invention comprises a central processing unit (CPU), input means, output means, and data storage means. One skilled in the art can readily appreciate that any currently available computer-based system is suitable for use in the present invention.

As stated above, the computer-based systems of the present invention comprise a data storage means having stored therein a nucleotide sequence of the present invention and the necessary hardware means and software means for supporting and implementing a search means.

The term “data storage means” is defined herein as memory which can store nucleotide sequence information of the present invention, or a memory access means which can access manufactures having recorded thereon the nucleotide sequence information of the present invention.

The term “search means” refers is defined herein as one or more programs which are implemented on the computer-based system to compare a target sequence or target structural motif with the sequence information stored within the data storage means. Search means are used to identify fragments or regions of the present genomic sequences which match a particular target sequence or target motif. A variety of known algorithms are disclosed publicly and a variety of commercially available software for conducting search means are and can be used in the computer-based systems of the present invention. Examples of such software includes, but is not limited to, MacPattern (Fuchs, 1991, Comput. Appl. Biosci. 7: 105-106), BLASTN and BLASTX National Center for Biotechnology Information (NCBI). One skilled in the art can readily recognize that any one of the available algorithms or implementing software packages for conducting homology searches can be adapted for use in the present computer-based systems.

The term “target sequence” is defined here as any DNA (genomic DNA, cDNA) or amino acid sequence of six or more nucleotides or two or more amino acids. One skilled in the art can readily recognize that the longer a target sequence is, the less likely a target sequence will be present as a random occurrence in the database. The most preferred sequence length of a target sequence is from about 10 to 100 amino acids or from about 30 to 300 nucleotide residues. However, it is well recognized that searches for commercially important fragments, such as sequence fragments involved in gene expression and protein processing, may be of shorter length.

The term “a target structural motif” or “target motif” is defined herein as any rationally selected sequence or combination of sequences in which the sequence(s) are chosen based on a three-dimensional configuration which is formed upon the folding of the target motif. There are a variety of target motifs known in the art. Protein target motifs include, but are not limited to, enzyme active sites and signal sequences, substrate and cofactor binding domains, transmembrane domains, and sites for post-translational modifications. Nucleic acid target motifs include, but are not limited to, promoter sequences, hairpin structures and inducible expression elements (protein binding sequences), repeats, palindromes, dyad symmetries, and transcription and translation start and stop sites.

A variety of structural formats for the input and output means can be used to input and output the information in the computer-based systems of the present invention. A preferred format for an output means ranks fragments of the Bacillus licheniformis or Bacillus clausii genomic sequences possessing varying degrees of homology to the target sequence or target motif. Such presentation provides one skilled in the art with a ranking of sequences which contain various amounts of the target sequence or target motif and identifies the degree of homology contained in the identified fragment.

A variety of comparing means can be used to compare a target sequence or target motif with the data storage means to identify sequence fragments of the Bacillus licheniformis and Bacillus clausii genomes. For example, implementing software which utilize the BLAST and BLAZE algorithms, described in Altschul at al., 1990, supra, may be used to identify open reading frames within the Bacillus licheniformis or Bacillus clausii genome or the genomes of other organisms. A skilled artisan can readily recognize that any one of the publicly available homology search programs can be used as the search means for the computer-based systems of the present invention. Suitable proprietary systems that may be known to those of skill also may be employed in this regard.

Codon Usage Tables

The present invention further relates to methods for preparing a synthetic gene, comprising (a) generating a codon usage table based on codons used in one or more open reading frames or portions thereof of SEQ ID NO: 1, (b) constructing a synthetic gene or portion thereof that contains in place of one or more native codons one or more preferred codons from the codon usage table, and (c) recovering the synthetic gene. In a preferred aspect, the codon usage table is Table 4 and/or Table 5.

The Bacillus licheniformis chromosomal sequence of SEQ ID NO: 1 or portions thereof can be used to generate codon usage tables to design synthetic genes for their efficient heterologous expression in Bacillus licheniformis host cells. The codon usage tables can be based on (1) the codon used in all the open reading frames, (2) selected open reading frames, (3) fragments of the open reading frames, or (4) fragments of selected open reading frames. With a codon usage table, synthetic genes can be designed with only the most preferred codon for each amino acid; with a number of common codons for each amino acid; or with the same or a similar statistical average of codon usages found in the table of choice.

The synthetic gene can be constructed using any method such as site-directed mutagenesis or PCR generated mutagenesis in accordance with methods known in the art. Although, in principle, the modification may be performed in vivo, i.e., directly on the cell expressing the nucleotide sequence to be modified, it is preferred that the modification is performed in vitro.

The synthetic gene can be further modified by operably linking the synthetic gene to one or more control sequences which direct the expression of the coding sequence in a suitable host cell under conditions compatible with the control sequences using the methods described herein. Nucleic acid constructs, recombinant expression vectors, and recombinant host cells comprising the synthetic gene can also be prepared using the methods described herein.

The present invention also relates to methods for producing a polypeptide encoded by such a synthetic gene comprising (a) cultivating a host cell comprising the synthetic gene under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.

The present invention is further described by the following examples which should not be construed as limiting the scope of the invention.

EXAMPLES
Example 1
Shotgun DNA Sequencing and Genome Assembly

The genome of the type strain Bacillus licheniformis ATCC 14580 was sequenced by a combination of the whole genome shotgun method described by Wilson, R. K. and Mardis, E. R., 1997, In Genome Analysis: A Laboratory Manual, Vol. 1, eds. Birren, B., Green, E. D., Meyers, R. M., and Roskams, J. (Cold Spring Harbor Press, Cold Spring Harbor, N.Y.), pp. 397-454, and fosmid end sequencing (Kim, U. J., Shizuya, H., de Jong, P. J., Birren, B. and Simon, M. I., 1992, Nucleic Acids Res. 20: 1083-1085; Longmire, J. L. and Brown, N. C., 2003, Biotechniques 35: 50-54; Zhao, S., Malek, J., Mahairas, G., Fu, L., Nierman, W., Venter, J. C., and Adams, M. D., 2000, Genomics 63: 321-332).

Genomic DNA of Bacillus licheniformis ATCC 14580 was isolated using the following method; A single colony was used to inoculate 20 ml of LB broth (Davis, R. W., Botstein, D., and Roth, J. R. 1980, Advanced Bacterial Genetics, Cold Spring Harbor Press, Cold Spring Harbor, N.Y.) in a sterile 125 ml Erlenmeyer flask. The culture was incubated at 37° C. overnight with agitation at 240 rpm. The resulting cells were collected by centrifugation in a 45 ml Oak Ridge tube for 10 minutes at 6000×g, and the cell pellet was resuspended in 5 ml of Tris-glucose buffer (50 mM Tris-HCl, pH 8.0, 50 mM glucose, 10 mM EDTA). Lysozyme was added to a final concentration of 50 μg/ml and the suspension was incubated in a 37° C. water bath for 25 minutes. Next, 200 μl of 10% SDS was added and the tubes were gently inverted several times. Five milliliters of a second detergent mixture (1% Brij, 1% deoxycholate, 50 mM EDTA, pH 7.5) was added, and the tubes were inverted several times while incubating for 20 minutes at room temperature. An equal volume of phenol:chloroform (1:1 v/v) was added and the tubes were inverted gently at room temperature for 20-30 minutes. The tubes were centrifuged for 20 minutes at 12,000×g, 4° C. The top aqueous layer was carefully removed with a wide-bore pipette and placed in a clean 45 ml Oak Ridge tube. The phenol:chloroform extraction was repeated and 1/10 volume of 3 M sodium acetate pH 5.2 was added to the aqueous layer. Two volumes of cold ethanol were carefully layered on top and the DNA was spooled from the solution onto a sterile glass rod. Spooled DNA was carefully rinsed in 70% ethanol and resuspended in a suitable amount of TE buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA).

Plasmid libraries were constructed using randomly-sheared and BamHI-digested genomic DNA that was enriched for 2-3 kb fragments by preparative agarose gel electrophoresis (Berka, R. M., Schneider, P., Golightly, E. J., Brown, S. H., Madden, M., Brown, K. M., Halkier, T., Mondorf, K., and Xu, F., 1997, Appl. Environ. Microbiol. 63: 3151-3157). Approximately 49,000 random clones were sequenced using dye-terminator chemistry (Applied Biosystems, Foster City, Calif.) with ABI 377 and ABI 3700 automated sequencers yielding approximately 6× coverage of the genome. A combination of methods was employed for gap closure including sequencing on fosmids (Kim, U. J., Shizuya, H., de Jong, P. J., Birren, B., and Simon, M. I., 1992, Nucleic Acids Res. 20: 1083-1085), primer walking on selected clones, and PCR-amplified DNA fragments. Fosmid libraries were constructed using a commercial kit from Epicentre (Madison, Wis.). Data from both ends of approximately 1975 fosmid clones with an average insert size of 40 kb were incorporated to aid in validation of the final assembly. In total, the number of input reads was 62,685 with 78.6% of these incorporated into the final assembly. Sequences were base called using TraceTuner 2.0 (Paracel, Inc., Pasadena, Calif.) and assembled using the Paracel Genome Assembler (Paracel, Inc., Pasadena, Calif.) with optimized parameters and the quality score set to >20. Phrap, Crossmatch, and Consed were used for sequence finishing (Gordon D., Abajian C., and Green P., 1998, Genome Res. 8: 195-202).

Example 2
Identification and Annotation of Open Reading Frames (ORFs)

Protein coding regions in the assembled genome sequence data were identified using EasyGene (Larsen, T. S., and Krogh, A., 2003, BMC Bioinformatics 4: 21), Glimmer (Delcher, A. L., Harmon, D., Kasif, S., White, O. and Salzberg, S. L., 1999, Nucleic Acids Res. 27, 4636-4641), and FrameD (Schiex, T., Gouzy, J., Moisan, A. and de Oliveira, Y., 2003, Nucleic Acids Res. 31, 3738-3741). Only EasyGene gene models with an R-value of less than 2 and log-odds score greater than −10 were used. Predicted proteins were compared to the non-redundant database PIR-NREF (Wu, C. H., Huang, H., Arminski, L., Castro-Alvear, J., Chen, Y., Hu, Z. Z., Ledley, R. S., Lewis, K. C., Mewes, H. W., Orcutt, B. C., 2002, Nucleic Acids Research 30: 35-37) and the Bacillus subtilis genome (SubtilList) using BLASTP with an E-value threshold of 10⁻⁵. InterProScan was used to predict putative function. (Zdobnov, E. M. and Apweiler, R., 2001, Bioinformatics 17, 847-848). The InterPro analysis included comparison to Pfam (Bateman, A., Coin, L., Durbin, R. Finn, R. D., Hollich, V., Griffiths-Jones, S., Khanna, A., Sonnhammer, E. L., et al., 2004, Nucleic Acids Res. 32, D138-D141), TIGRfam (Haft, D. J., Selengut, J. D. and White, O., 2003, Nucleic Acids Res. 31: 371-373), Interpro (Apweiler, R., Attwood, T. K., Bairock, A., Bateman, A., Birney, E., Biswas, M., Bucher, P., Cerutti, L., Corpet, F., Croning, M. D., et al., 2001, Nucleic Acids Res. 29: 37-40), signal peptide prediction using SignalP (Nielsen, H., Engelbrecht, J., Brunak, S., and von Heijne, G., 1997, Protein Engineering 10: 1-6), and trans-membrane domain prediction using TMHMM (Krogh, A., Larsson, B., von Heijne, G. and Sonnhammer, E. L. L., 2000, J. Mol. Biol. 305, 567-580).

These ORFs were assigned to functional categories based on the Cluster of Orthologous Groups (COG) database with manual verification as described (Tatusov, R. L., Koonin, E. V. and Lipman. D. J., 1997, Science 278: 631-637; Koonin, E. V. and Galperin, M. Y., 2002, Sequence-Evolution-Function: Computational Approaches in Comparative Genomics (Kluwer, Boston)). Transfer RNA genes were identified using tRNAscan-SE (Lowe, T. M. and Eddy, S. R., 1997, Nucleic Acids Res. 25: 955-964).

Example 3
General Features of the Bacillus licheniformis Genome

The genome of Bacillus licheniformis ATCC 14580 was determined to consist of a circular molecule of 4,222,336 bp with an average GC content of 46.2% (Table 2). No plasmids were found during the genome analysis, and none were found by agarose gel electrophoresis.

The genome contains 4208 predicted protein-coding genes with an average size of 873 bp, seven rRNA operons, and 81 tRNA genes. Using a combination of several gene-finding algorithms 4208 protein coding ORFs were predicted. These ORFs constitute 87% of the genome and have an average length of 873 bp. Approximately 48% of the ORFs are encoded on one DNA strand and 52% on the other strand. Among the protein coding ORFs, 3948 (94%) have significant similarity to proteins in PIR, and 3187 of these gene models contain Interpro motifs and 2895 contain protein motifs found in PFAM. The number of hypothetical and conserved hypothetical proteins in the Bacillus licheniformis genome with hits in the FIR database was 1318 (212 conserved hypothetical ORFs). Among the list of hypothetical and conserved hypothetical ORFs, 683 (52%) have protein motifs contained in PFAM (148 conserved hypothetical ORFs). There are 72 tRNA genes representing all 20 amino acids and 7 rRNA operons.

The likely origin of replication was identified by similarities to several features of Bacillus subtilis origin (Moriya, S., Fukuoka, T., Ogasawara, N., and Yoshikawa, H., 1988, EMBO Journal 7: 2911-2917; Ogasawara, N., Nakai, S., and Yoshikawa, H., 1994, DNA Res. 1, 1-14; Kadoya, R., Hassan, A. K., Kasahara, Y., Ogasawara, N., and Moriya, S., 2002, Mol. Microbiol. 45: 73-87; Tosato, V., Gjuracic, K., Vlahovicek, K., Pongor, S., Danchin, A., and Bruschi, C. V., 2003, FEMS Microbiol, Lett. 218: 23-30). These included (a) colocalization of four genes (rpmH, dnaA, dnaN, and recF) found near the origin of the Bacillus subtilis chromosome, (b) GC nucleotide skew [(G−C)/(G+C)] analysis, and (c) the presence of multiple dnaA-boxes (Pedersen, A. G., Jensen, L. J., Brunak, S., Staerfeldt, H. H., and Ussery, D. W., 2000, Mol. Biol. 299: 907-930; Christensen, B. B., Atlung, T., and Hansen, F. G., 1999, J. Bacterial. 181: 2683-2688; Majka, J., Jakimowicz, D., Messer, W., Schrempf, H., Lisowski, M., and Zakrzewska-Czerwinska, J., 1999, Eur. J. Biochem. 260: 325-335) and AT-rich sequences in the region immediately upstream of the dnaA gene. On the basis of these observations, a cytosine residue of the BstBI restriction site was assigned between the rpmH and dnaA genes to be the first nucleotide of the Bacillus licheniformis genome. The replication termination site was localized near 2.02 Mb by GC skew analysis. This region lies roughly opposite the origin of replication.

Unlike Bacillus subtilis, no apparent gene encoding a replication terminator protein (rtp) was found in Bacillus licheniformis. The Bacillus halodurans genome also lacks an rtp function (Takami, H., Nakasone, K., Takaki, Y., Maeno, G., Sasaki, R., Masui, N., Fuji, F., Hirama, C., Nakamura, Y., Ogasawara, N. et al., 2000, Nucleic Acids Res. 28: 4317-4331), and it seems likely that Bacillus subtilis acquired the rtp gene following its divergence from Bacillus halodurans and Bacillus licheniformis.

Transposable elements and prophages. The genome of Bacillus licheniformis ATCC 14580 was determined to contain nine identical copies of a 1285 bp insertion sequence element termed IS3Bli1 (Lapidus, A., Galleron, N., Andersen, J. T., Jørgensen, P. L. Ehrlich, S. D., and Sorokin, A., 2002, FEMS Microbiol. Lett. 209: 23-30). This sequence shares a number of features with other IS3 family elements including direct repeats of three to five bp, a ten bp left inverted repeat, and a nine by right inverted repeat. IS3Bli1 encodes two predicted overlapping ORFs, designated orfA and orfB in relative translational reading frames of 0 and −1. The presence of a “slippery heptamer” motif, AAAAAAG, before the stop codon in orfA suggests that programmed translational frameshifting occurs between these two ORFs, resulting in a single gene product (Farabaugh, P., 1996, Microbiol. Rev. 60: 103-134). The orfB gene product harbors the DD[35]E[7]K motif, a highly conserved pattern among insertion sequences. Eight of the IS3Bli1 elements lie in intergenic regions, and one interrupts the comP gene. In addition to these insertion sequences, the genome encodes a putative transposase that is most closely related (E=1.8e-11) to one identified in the Thermoanaerobacter tengcongensis genome (Bao, Q., Tian, Y., Li, W., Xu, Z.; Xuan, Z., Hu, S., Dong, W., Yang, J., Chen, Y., Xue, Y., et al., 2002, Genome Res. 12: 689-700), however, similar genes are also found in the chromosomes of Bacillus halodurans (Takaki, H., Nakasone, K., Takaki, Y., Maeno, G., Sasaki, R., Masui, N., Fuji, F., Hirama, C., Nakamura, Y., Ogasawara, N. et al., 2000, Nucleic Acids Res. 28: 4317-4331), Oceanobacillus iheyensis (Takagi, H. Takaki, Y., and Uchiyama, I., 2002, Nucleic Acids Res. 30: 3927-3935), Streptococcus agalactiae (Takahashi, S., Detrick, S., Whiting, A. A, Blaschke-Bonkowksy, A. J., Aoyagi, Y., Adderson, E. E., and Bohnsack, J. F., 2002, J. Infect. Dis. 186: 1034-1038), and Streptococcus pyogenes (Smoot, J. C., Barbian, K. D., Van Gompel, J. J., Smoot, L. M., Chaussee, M. S., Sylva, G. L., Sturdevant, D. E., Ricklefs, S. M., Porcella, S. F., Parkins, L. D., et al., 2002, Proc. Natl. Acad. Sci. U.S.A. 99: 4668-4673).

The presence of several bacteriophage lysogens or prophage-like elements was revealed by Smith-Waterman comparisons to other bacterial genomes and by their AT-rich signatures. Prophage sequences, designated NZP1 and NZP3 (similar to PBSX and φ-105, respectively), were uncovered by noting the presence of nearby genes encoding the large subunit of terminase, a signature protein that is highly conserved among prophages (Casjens, S., 2003, Mol. Microbiol. 49: 277-300). A terminase gene was not observed in a third putative prophage, termed NZP2 (similarity to SPP1), however, its absence may be the result of genome deterioration during evolution. Regions were observed in which the GC content is less than 39% usually encoded proteins that have no Bacillus subtilis orthologue and share identity only to hypothetical and conserved hypothetical genes. Two of these AT-rich segments correspond to the NZP2 and NZP3 prophages.

An isochore plot also revealed the presence of a region with an atypically high (62%) G+C content. This segment contains two hypothetical ORFs whose sizes (3831 and 2865 bp) greatly exceed the size of an average gene in Bacillus licheniformis. The first protein encodes a protein of 1277 amino acids for which Interpro predicted 16 collagen triple helix repeats, and the amino acid pattern TGATGPT is repeated 75 times within the polypeptide. The second ORF is smaller, and encodes a protein with 11 collagen triple helix repeats, and the same TGATGPT motif recurs 56 times. Interestingly, the chromosomal region (19 kb) adjacent to these genes is clearly non-colinear with the Bacillus subtilis genome, and virtually all of the predicted ORFs are hypothetical or conserved hypothetical proteins. There are a number of bacterial proteins listed in PIR that contain collagen triple helix repeat regions including two from Mesorhizobium loti (accession numbers NF00607049 and NF00607035) and three from Bacillus cereus (accession numbers NF01692528, NF01269899, and NF01694666). These putative orthologs share 53-76% amino acid sequence identity with their counterparts in Bacillus licheniformis, although their functions are unknown.

Extracellular enzymes. In the Bacillus licheniformis genome, 689 of the 4208 gene models have signal peptides as forecasted by SignalP (Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites, Henrik Nielsen, Jacob Engelbrecht, Søren Brunak and Gunnar von Heijne, 1997, Protein Engineering 10: 1-6). Of these, 309 have no trans-membrane domain as predicted with TMHMM (A. Krogh, B. Larsson, G. von Heijne, and E. L. L. Sonnhammer, 2000, Journal of Molecular Biology 305: 567-580) and 134 are hypothetical or conserved hypothetical genes. Based on a manual examination of the remaining 175 ORFs, at least 82 were determined to likely encode secreted proteins and enzymes. The sequence ID numbers for each of these genes are listed in Table 3.

Protein secretion, sporulation, and competence pathways. Kunst et al. (Kunst, F., Ogasawara, N, Mozser, I., Albertini, A. M., Alloni, G., Azebedo, V., Bertero, M. G., Bessieres, P., Bolotin, A., and Borchert, S., 1997, Nature 390: 249-256) listed 18 genes that play a major role in the secretion of extracellular enzymes by Bacillus subtilis 168. This list includes several chaperonins, signal peptidases, components of the signal recognition particle and protein translocase complexes. Thus, it is reasonable to expect that the central features of the secretory apparatus are conserved in Bacillus subtilis and Bacillus licheniformis.

From the list of 139 sporulation genes tabulated by Kunst et al. (Kunst, F., Ogasawara, N, Mozser, I., Albertini, A. M., Alloni, G., Azebedo, V., Bertero, M. G., Bessieres, P., Bolotin, A., and Borchert, S. et al., 1997, Nature 390: 249-256), all but six have obvious counterparts in Bacillus licheniformis. These six exceptions (spsABCEFG) comprise an operon involved in synthesis of spore coat polysaccharide in Bacillus subtilis. Additionally, the response regulator gene family (phrACEFGI) appears to have a low level of sequence conservation between Bacillus subtilis and Bacillus licheniformis.

Natural competence (the ability to take up and process exogenous DNA in specific growth conditions) is a feature of few Bacillus licheniformis strains (Gwinn, D. D. and Thorne, C. B., 1964, J. Bacteriol. 87: 519-526). The reasons for variability in competence phenotype have not been explored at the genetic level, but the genome data offered several possible explanations. Although the type strain genome encodes all of the late competence functions ascribed in Bacillus subtilis (e.g., comC, comEFG operons, comK, mecA), it lacks an obvious comS gene, and the comP gene is punctuated by an insertion sequence element, suggesting that the early stages of competence development have been preempted in Bacillus licheniformis ATCC 14580. Whether these early functions can be restored by introducing the corresponding genes from Bacillus subtilis is unknown. In addition to an apparent deficiency in DNA uptake, two Type I restriction-modification systems were discovered that may also contribute diminished transformation efficiencies. These are distinct from the ydiOPS genes of Bacillus subtilis, and could participate in degradation of improperly modified DNA from heterologous hosts used during construction of recombinant expression vectors. Lastly, the synthesis of a glutamyl polypeptide capsule has also been implicated as a potential barrier to transformation of Bacillus licheniformis strains (Thorne, C. B. and Stull, H. B., 1966, J. Bacteriol. 91: 1012-1020). Six genes were predicted (ywtABDEF and ywsC orthologues) that may be involved in the synthesis of this capsular material.

Antibiotics and secondary metabolites. Bacitracin is a cyclic peptide antibiotic that is synthesized non-ribosomally in Bacillus licheniformis (Katz, E. and Demain, A. L., 1977, Bacteriol. Rev. 41: 449-474). While there is variation in the prevalence of bacitracin synthase genes in laboratory strains of this species, one study suggested that up to 50% may harbor the bac operon (Ishihara, H., Takoh, M., Nishibayashi, R., and Sato, A., 2002, Curr. Microbiol. 45: 18-23). The bac operon was determined not to be present in the type strain (ATCC 14580) genome. Seemingly, the only non-ribosomal peptide synthase operon encoded by the Bacillus licheniformis type strain genome is that which is responsible for lichenysin biosynthesis. Lichenysin structurally resembles surfactin from Bacillus subtilis (Grangemard, I., Wallach, J., Maget-Dana, R., and Peypoux, F., 2001, Appl. Biochem. Biotechnol. 90: 199-210), and their respective biosynthetic operons are highly similar. No Bacillus licheniformis counterparts were found for the pps (plipastatin synthase) and polyketide synthase (pks) operons of Bacillus subtilis. Collectively, these two regions represent sizeable portions (80 kb and 38 kb, respectively) of the chromosome in Bacillus subtilis, although they are reportedly dispensable (Westers, H., Dorenbos, R., van Dijl, J. M., Kable, J., Flanagan, T., Devine, K. M., Jude, F., Seror, S. J., Beekman, A. C., Darmon, E., 2003, Mol. Biol. Evol. 20: 2076-2090). Unexpectedly, a gene cluster was found encoding a lantibiotic and associated processing and transport functions. This peptide of 69 amino acids was designated as lichenicidin, and its closest known orthologue is mersacidin from Bacillus sp. strain HIL-Y85/54728 (Altena, K., Guder, A., Cramer, C., and Bierbaum, G., 2000, Appl. Environ. Microbiol. 66: 2565-2571). Lantibiotics are ribosomally synthesized peptides that are modified post-translationally so that the final molecules contain rare thioether amino acids such as lanthionine and/or methyl-lanthionine (Pag, U. and Sahl, H. G., 2002, Curr. Pharm. Des. 8: 815-833). These antimicrobial compounds have attracted much attention in recent years as models for the design of new antibiotics (Hoffmann, A., Pag, U., Wiedemann, I., and Sahl, H. G., 2002, Farmaco. 57: 685-691).

Essential Genes. The gene models were also compared to the list of essential genes in Bacillus subtilis (Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., Asai, K., Ashikaga, S., Aymerch, S., Bessieres, P., 2003, Proc. Natl Acad. Sci. USA 100: 4678-4683). All the essential genes in Bacillus subtilis have orthologues in Bacillus licheniformis, and most are present in a wide range of bacterial taxa (Pedersen, P. B., Bjørnvad, M. E., Rasmussen, M. D., and Petersen, J. N., 2002, Reg. Toxicol. Pharmacol. 36: 155-161).

Example 4
Comparison of Bacillus licheniformis Genome with Other Bacilli

VisualGenome software (Rational Genomics, San Francisco, Calif.) was used for GC-skew analysis and global homology comparisons of the Bacillus licheniformis, Bacillus subtilis, and Bacillus halodurans genomes with pre-computed BLAST results stored in a local database. In pairwise comparisons (E-score threshold of 10⁻⁵) 66% (2771/4208) of the predicted Bacillus licheniformis ORFs have orthologs in Bacillus subtilis, and 55% (2321/4208) of the gene models are represented by orthologous sequences in Bacillus halodurans. Using a reciprocal BLASTP analysis 1719 orthologs were found that are common to all three species (E-score threshold of 10⁻⁵).

As noted by Lapidus et al. (Lapidus, A., Galleron, N., Andersen, J. T., Jørgensen, P. L. Ehrlich, S. D., and Sorokin, A., 2002, FEMS Microbiol. Lett. 209: 23-30), there are broad regions of colinearity between the genomes of Bacillus licheniformis and Bacillus subtilis. Less conservation of genome organization exists between Bacillus licheniformis and Bacillus halodurans, and inversion of one or more large genomic segments is evident. Clearly this supports previous findings (Xu, D. and Côté, J. C., 2003, Internat. J. Syst. Evol. Microbiol. 53: 695-704) that Bacillus subtilis and Bacillus licheniformis are phylogenetically and evolutionarily closer than either species is to Bacillus halodurans. However, a number of important differences were also observed, both in the numbers and locations of prophages and transposable elements and in a number of biochemical pathways, which distinguish Bacillus licheniformis from Bacillus subtilis, including a region of more than 80 kb that comprises a cluster of polyketide synthase genes that are absent in Bacillus licheniformis.

Example 5
Codon Usage Tables

The evolution of codon bias, the unequal usage of synonomous codons, is thought to be due to natural selection for the use of preferred codons that match the most abundant species of isoaccepting tRNAs, resulting in increased translational efficiency and accuracy. The practical applications for utilizing codon bias information include optimizing expression of heterologous and mutant genes (Jiang and Mannervik, 1999, Protein Expression and Purification 15: 92-98), site-directed mutagenesis to derive variant polypeptides from a given gene (Wong at al., 1995. J. Immunol. 154: 3351-3358; Kaji, H. et al., 1999, J. Biochem. 126: 769-775), design and synthesis of synthetic genes (Libertini and Di Donato, 1992, Protein Engineering 5: 821-825: Feng et al., 2000, Biochem. 39: 15399-15409), and fine-tuning or reducing of translation efficiency of specific genes by introduction of non-preferred codons (Crombie, T. et al., 1992, J. Mol. Biol. 228: 7-12; Carlini and Stephan, 2003, Genetics 163: 239-243).

A codon usage table (Table 4) was generated from SEQ ID NO: 1 with CUSP, a software component of the EMBOSS package (Rice, Longden, and Bleasby, 2000, EMBOSS: The European Molecular Biology Open Software Suite. Trends in Genetics 16: 276-277) on all the predicted protein-coding genes of the Bacillus licheniformis chromosome. CUSP read the coding sequences and calculated the codon frequency table shown in Table 4.

A codon usage table (Table 5) was also generated based on the signal peptides of the 82 extracellular proteins described in Example 3.

TABLE 1

Predicted functions

Bacillus

subtilis

SEQ

homolog

ID

(Gene

NO.
Description
UniRef Accession No.
Organism
Name)

2
Chromosomal replication initiator
UniRef100_P05648

Bacillus subtilis

DnaA

protein dnaA [Bacillus subtilis]

3
DNA polymerase III, beta chain
UniRef100_P05649

Bacillus subtilis

DnaN

[Bacillus subtilis]

4

5
DNA replication and repair protein recF
UniRef100_P05651

Bacillus subtilis

RecF

[Bacillus subtilis]

6

7
DNA gyrase subunit B [Bacillus subtilis]
UniRef100_P05652

Bacillus subtilis

GyrB

8
DNA gyrase subunit A [Bacillus subtilis]
UniRef100_P05653

Bacillus subtilis

GyrA

9

YaaC

10
Inosine-5′-monophosphate
UniRef100_P21879

Bacillus subtilis

GuaB

dehydrogenase [Bacillus subtilis]

11
D-alanyl-D-alanine carboxypeptidase
UniRef100_P08750

Bacillus subtilis

DacA

precursor [Bacillus subtilis]

12
Pyridoxine biosynthesis protein pdx1
UniRef100_P37527

Bacillus subtilis

YaaD

[Bacillus subtilis]

13
Hypothetical UPF0030 protein yaaE
UniRef100_P37528

Bacillus subtilis

YaaE

[Bacillus subtilis]

14
Seryl-tRNA synthetase [Bacillus
UniRef100_P37464

Bacillus subtilis

SerS

subtilis]

15
Glycerate kinase [Bacillus subtilis]
UniRef100_P42100

Bacillus subtilis

YxaA

16
H+/gluconate symporter [Vibrio
UniRef100_Q7MHW6

Vibrio vulnificus

YuiF

vulnificus]

17
Sugar diacid utilization regulator [Vibrio
UniRef100_Q8DBZ9

Vibrio vulnificus

YsfB

vulnificus]

18
Hypothetical protein [Bacillus
UniRef100_Q6HH43

Bacillus thuringiensis

thuringiensis]

19
Hypothetical protein yaaF [Bacillus
UniRef100_P37529

Bacillus subtilis

Dck

subtilis]

20
Hypothetical protein yaaG [Bacillus
UniRef100_P37530

Bacillus subtilis

Dgk

subtilis]

21
Hypothetical protein yaaH [Bacillus
UniRef100_P37531

Bacillus subtilis

YaaH

subtilis]

22
Hypothetical protein yaaI [Bacillus
UniRef100_P37532

Bacillus subtilis

YaaI

subtilis]

23

YaaJ

24

DnaX

25
Hypothetical UPF0133 protein yaaK
UniRef100_P24281

Bacillus subtilis

YaaK

[Bacillus subtilis]

26
Recombination protein recR [Bacillus
UniRef100_P24277

Bacillus subtilis

RecR

subtilis]

27
Hypothetical protein yaaL [Bacillus
UniRef100_P37533

Bacillus subtilis

subtilis]

28
Sigma-K factor processing regulatory
UniRef100_P24282

Bacillus subtilis

protein BOFA [Bacillus subtilis]

29
CsfB protein [Bacillus subtilis]
UniRef100_P37534

Bacillus subtilis

30
XpaC protein [Bacillus subtilis]
UniRef100_P37467

Bacillus subtilis

XpaC

31
Hypothetical protein yaaN [Bacillus
UniRef100_P37535

Bacillus subtilis

YaaN

subtilis]

32

YaaO

33
Thymidylate kinase [Bacillus subtilis]
UniRef100_P37537

Bacillus subtilis

Tmk

34
Hypothetical protein yaaQ [Bacillus
UniRef100_P37538

Bacillus subtilis

YaaQ

subtilis]

35

36
DNA polymerase III, delta′ subunit
UniRef100_P37540

Bacillus subtilis

HolB

[Bacillus subtilis]

37
Hypothetical protein yaaT [Bacillus
UniRef100_P37541

Bacillus subtilis

YaaT

subtilis]

38
Hypothetical protein yabA [Bacillus
UniRef100_P37542

Bacillus subtilis

YabA

subtilis]

39
Hypothetical protein yabB [Bacillus
UniRef100_P37543

Bacillus subtilis

YabB

subtilis]

40
Hypothetical UPF0213 protein yazA
UniRef100_O31414

Bacillus subtilis

[Bacillus subtilis]

41
Hypothetical UPF0011 protein yabC
UniRef100_P37544

Bacillus subtilis

YabC

[Bacillus subtilis]

42
Transition state regulatory protein abrB
UniRef100_P08874

Bacillus subtilis

[Bacillus subtilis]

43
Methionyl-tRNA synthetase [Bacillus
UniRef100_P37465

Bacillus subtilis

MetS

subtilis]

44
Putative deoxyribonuclease yabD
UniRef100_P37545

Bacillus subtilis

YabD

[Bacillus subtilis]

45

YabE

46
Hypothetical protein yabF [Bacillus
UniRef100_P37547

Bacillus subtilis

RnmV

subtilis]

47
Dimethyladenosine transferase (EC
UniRef100_P37468

Bacillus subtilis

KsgA

2.1.1.—) (S-adenosylmethionine-6-N′,N′-

adenosyl(rRNA) dimethyltransferase)

[Bacillus subtilis]

48
Hypothetical protein yabG [Bacillus
UniRef100_P37548

Bacillus subtilis

YabG

subtilis]

49
Veg protein [Bacillus subtilis]
UniRef100_P37466

Bacillus subtilis

50
SspF protein [Bacillus subtilis]
UniRef100_P37549

Bacillus subtilis

51

IspE

52
Pur operon repressor [Bacillus subtilis]
UniRef100_P37551

Bacillus subtilis

PurR

53
UPF0076 protein yabJ [Bacillus subtilis]
UniRef100_P37552

Bacillus subtilis

YabJ

54
Stage V sporulation protein G [Bacillus
UniRef100_P28015

Bacillus subtilis

subtilis]

55
Bifunctional gcaD protein (TMS protein)
UniRef100_P14192
Includes; UDP-
GcaD

[Includes: UDP-N-acetylglucosamine

N-

pyrophosphorylase (EC 2.7.7.23) (N-

acetylglucosamine

acetylglucosamine-1-phosphate

pyrophosphorylase

uridyltransferase); Glucosamine-1-

(EC 2.7.7.23)

phosphate N-acetyltransferase (EC

(N-

2.3.1.157)] [Bacillus subtilis]

acetylglucosamine-

1-phosphate

uridyltransferase);

Glucosamine-1-

phosphate N-

acetyltransferase

(EC 2.3.1.157)

56
Ribose-phosphate pyrophosphokinase
UniRef100_P14193

Bacillus subtilis

Prs

[Bacillus subtilis]

57
General stress protein ctc [Bacillus
UniRef100_P14194

Bacillus subtilis

Ctc

subtilis]

58
Peptidyl-tRNA hydrolase [Bacillus
UniRef100_P37470

Bacillus subtilis

SpoVC

subtilis]

59
Hypothetical protein yabK [Bacillus
UniRef100_P37553

Bacillus subtilis

subtilis]

60
Transcription-repair coupling factor
UniRef100_P37474

Bacillus subtilis

Mfd

[Bacillus subtilis]

61
Stage V sporulation protein T [Bacillus
UniRef100_P37554

Bacillus subtilis

SpoVT

subtilis]

62
Hypothetical protein yabM [Bacillus
UniRef100_P37555

Bacillus subtilis

YabM

subtilis]

63
Hypothetical protein yabN [Bacillus
UniRef100_P37556

Bacillus subtilis

YabN

subtilis]

64
Hypothetical protein yabO [Bacillus
UniRef100_P37557

Bacillus subtilis

subtilis]

65
Hypothetical protein yabP [Bacillus
UniRef100_P37558

Bacillus subtilis

YabP

subtilis]

66
Hypothetical protein yabQ [Bacillus
UniRef100_P37559

Bacillus subtilis

YabQ

subtilis]

67
Cell division protein divlC [Bacillus
UniRef100_P37471

Bacillus subtilis

DivlC

subtilis]

68
Hypothetical protein yabR [Bacillus
UniRef100_P37560

Bacillus subtilis

YabR

subtilis]

69
Stage II sporulation protein E [Bacillus
UniRef100_P37475

Bacillus subtilis

SpoIIE

subtilis]

70
Hypothetical protein yabS [Bacillus
UniRef100_P37561

Bacillus subtilis

YabS

subtilis]

71
Probable serine/threonine-protein
UniRef100_P37562

Bacillus subtilis

YabT

kinase yabT [Bacillus subtilis]

72
Hypothetical UPF0072 protein yacA
UniRef100_P37563

Bacillus subtilis

YacA

[Bacillus subtilis]

73
Hypoxanthine-guanine
UniRef100_P37472

Bacillus subtilis

HprT

phosphoribosyltransferase [Bacillus

subtilis]

74
Cell division protein ftsH homolog
UniRef100_P37476

Bacillus subtilis

FtsH

[Bacillus subtilis]

75
Putative 32 kDa replication protein
UniRef100_Q9F985

Bacillus

YacB

[Bacillus stearothermophilus]

stearothermophilus

76
33 kDa chaperonin [Bacillus subtilis]
UniRef100_P37565

Bacillus subtilis

YacC

77

YacD

78

CysK

79
Para-aminobenzoate synthase
UniRef100_P28820

Bacillus subtilis

PabB

component I [Bacillus subtilis]

80
Para-aminobenzoate/anthranilate
UniRef100_P28819
Includes: Para-
PabA

synthase glutamine amidotransferase

aminobenzoate

component II [Includes; Para-

synthase

aminobenzoate synthase glutamine

glutamine

amidotransferase component II (EC

amidotransferase

6.3.5.8) (ADC synthase); Anthranilate

component II

synthase component II (EC 4.1.3.27)]

(EC 6.3.5.8)

[Bacillus subtil

(ADC synthase);

Anthranilate

synthase

component II

(EC 4.1.3.27)

81
Aminodeoxychorismate lyase [Bacillus
UniRef100_P28821

Bacillus subtilis

PabC

subtilis]

82
Dihydropteroate synthase [Bacillus
UniRef100_P28822

Bacillus subtilis

SuI

subtilis]

83
Dihydroneopterin aldolase [Bacillus
UniRef100_P28823

Bacillus subtilis

FolB

subtilis]

84
2-amino-4-hydroxy-6-
UniRef100_P29252

Bacillus subtilis

FolK

hydroxymethyldihydropteridine

pyrophosphokinase [Bacillus subtilis]

85
YazB protein [Bacillus subtilis]
UniRef100_O31417

Bacillus subtilis

86
Probable tRNA-dihydrouridine synthase
UniRef100_P37567

Bacillus subtilis

YacF

1 [Bacillus subtilis]

87
Lysyl-tRNA synthetase [Bacillus
UniRef100_P37477

Bacillus subtilis

LysS

subtilis]

88
Transcriptional regulator ctsR [Bacillus
UniRef100_P37568

Bacillus subtilis

CtsR

subtilis]

89
Hypothetical protein yacH [Bacillus
UniRef100_P37569

Bacillus subtilis

McsA

subtilis]

90
Hypothetical ATP:guanido
UniRef100_P37570

Bacillus subtilis

McsB

phosphotransferase yacI [Bacillus

subtilis]

91
Negative regulator of genetic
UniRef100_P37571

Bacillus subtilis

ClpC

competence clpC/mecB [Bacillus

subtilis]

92

RadA

93
Hypothetical protein yacK [Bacillus
UniRef100_P37573

Bacillus subtilis

YacK

subtilis]

94
Hypothetical protein yacL [Bacillus
UniRef100_Q06754

Bacillus subtilis

YacL

subtilis]

95
2-C-methyl-D-erythritol 4-phosphate
UniRef100_Q06755

Bacillus subtilis

YacM

cytidylyltransferase [Bacillus subtilis]

96
2-C-methyl-D-erythritol 2,4-
UniRef100_Q06756

Bacillus subtilis

YacN

cyclodiphosphate synthase [Bacillus

subtilis]

97
Glutamyl-tRNA synthetase [Bacillus
UniRef100_P22250

Bacillus subtilis

GltX

subtilis]

98
Serine acetyltransferase [Bacillus
UniRef100_Q06750

Bacillus subtilis

CysE

subtilis]

99
Cysteinyl-tRNA synthetase [Bacillus
UniRef100_Q06752

Bacillus subtilis

CysS

subtilis]

100
YazC protein [Bacillus subtilis]
UniRef100_O31418

Bacillus subtilis

YazC

101
Hypothetical tRNA/rRNA
UniRef100_Q06753

Bacillus subtilis

YacO

methyltransferase yacO [Bacillus

subtilis]

102
Hypothetical protein yacP [Bacillus
UniRef100_P37574

Bacillus subtilis

YacP

subtilis]

103
RNA polymerase sigma-H factor
UniRef100_P17869

Bacillus subtilis

SigH

[Bacillus subtilis]

104
Preprotein translocase secE subunit
UniRef100_Q06799

Bacillus subtilis

[Bacillus subtilis]

105

NusG

106
50S ribosomal protein L11 [Bacillus
UniRef100_Q06796

Bacillus subtilis

RplK

subtilis]

107
50S ribosomal protein L1 [Bacillus
UniRef100_Q06797

Bacillus subtilis

RplA

subtilis]

108
50S ribosomal protein L10 [Bacillus
UniRef100_P42923

Bacillus subtilis

RplJ

subtilis]

109
50S ribosomal protein L7/L12 [Bacillus
UniRef100_P02394

Bacillus subtilis

RplL

subtilis]

110
Hypothetical protein ybxB [Bacillus
UniRef100_P37872

Bacillus subtilis

YbxB

subtilis]

111
DNA-directed RNA polymerase beta
UniRef100_P37870

Bacillus subtilis

RpoB

chain [Bacillus subtilis]

112
DNA-directed RNA polymerase beta′
UniRef100_P37871

Bacillus subtilis

RpoC

chain [Bacillus subtilis]

113
Putative ribosomal protein L7Ae-like
UniRef100_P46350

Bacillus subtilis

[Bacillus subtilis]

114
30S ribosomal protein S12 [Bacillus
UniRef100_P21472

Bacillus subtilis

RpsL

subtilis]

115
30S ribosomal protein S7 [Bacillus
UniRef100_P21469

Bacillus subtilis

RpsG

subtilis]

116
Elongation factor G [Bacillus subtilis]
UniRef100_P80868

Bacillus subtilis

FusA

117
Elongation factor Tu [Bacillus subtilis]
UniRef100_P33166

Bacillus subtilis

TufA

118
30S ribosomal protein S10 [Bacillus
UniRef100_Q9Z9L5

Bacillus

RpsJ

halodurans]

halodurans

119
50S ribosomal protein L3 [Bacillus
UniRef100_P42920

Bacillus subtilis

RplC

subtilis]

120
50S ribosomal protein L4 [Bacillus
UniRef100_P42921

Bacillus subtilis

RplD

subtilis]

121
50S ribosomal protein L23 [Bacillus
UniRef100_P42924

Bacillus subtilis

subtilis]

122
50S ribosomal protein L2 [Bacillus
UniRef100_P42919

Bacillus subtilis

RplB

subtilis]

123
30S ribosomal protein S19 [Bacillus
UniRef100_P21476

Bacillus subtilis

subtilis]

124
50S ribosomal protein L22 [Bacillus
UniRef100_P42060

Bacillus subtilis

RplV

subtilis]

125
30S ribosomal protein S3 [Bacillus
UniRef100_P21465

Bacillus subtilis

RpsC

subtilis]

126
50S ribosomal protein L16 [Bacillus
UniRef100_P14577

Bacillus subtilis

RplP

subtilis]

127
50S ribosomal protein L29 [Bacillus
UniRef100_P12873

Bacillus subtilis

subtilis]

128
30S ribosomal protein S17 [Bacillus
UniRef100_P12874

Bacillus subtilis

subtilis]

129

130
50S ribosomal protein L24 [Bacillus
UniRef100_P12876

Bacillus subtilis

RplX

subtilis]

131
50S ribosomal protein L5 [Bacillus
UniRef100_P12877

Bacillus subtilis

RplE

subtilis]

132
Ribosomal protein S14 [Bacillus cereus
UniRef100_Q63H77

Bacillus cereus ZK

ZK]

133
30S ribosomal protein S8 [Bacillus
UniRef100_P12879

Bacillus subtilis

RpsH

subtilis]

134
50S ribosomal protein L6 [Bacillus
UniRef100_P46898

Bacillus subtilis

RplF

subtilis]

135
50S ribosomal protein L18 [Bacillus
UniRef100_P46899

Bacillus subtilis

RplR

subtilis]

136
30S ribosomal protein S5 [Bacillus
UniRef100_P21467

Bacillus subtilis

RpsE

subtilis]

137
50S ribosomal protein L30 [Bacillus
UniRef100_P19947

Bacillus subtilis

subtilis]

138
50S ribosomal protein L15 [Bacillus
UniRef100_P19946

Bacillus subtilis

RplO

subtilis]

139

SecY

140
Adenylate kinase [Bacillus subtilis]
UniRef100_P16304

Bacillus subtilis

Adk

141
Methionine aminopeptidase [Bacillus
UniRef100_P19994

Bacillus subtilis

Map

subtilis]

142
C-125 initiation factor IF-I, RNA
UniRef100_O50629

Bacillus halodurans

polymerase alpha subunit and

ribosomal proteins, partial and

complete cds [Bacillus halodurans]

143
Translation initiation factor IF-1
UniRef100_P20458

Bacillus subtilis

[Bacillus subtilis]

144

145

RpsM

146
30S ribosomal protein S11 [Bacillus
UniRef100_P04969

Bacillus subtilis

RpsK

subtilis]

147
DNA-directed RNA polymerase alpha
UniRef100_P20429

Bacillus subtilis

RpoA

chain [Bacillus subtilis]

148
50S ribosomal protein L17 [Bacillus
UniRef100_P20277

Bacillus subtilis

RplQ

subtilis]

149
Hypothetical ABC transporter ATP-
UniRef100_P40735

Bacillus subtilis

YbxA

binding protein ybxA [Bacillus subtilis]

150
Hypothetical protein orf5 [Bacillus
UniRef100_P70970

Bacillus subtilis

YbaE

subtilis]

151
YbaF protein [Bacillus subtilis]
UniRef100_P70972

Bacillus subtilis

YbaF

152
tRNA pseudouridine synthase A
UniRef100_P70973

Bacillus subtilis

TruA

[Bacillus subtilis]

153
50S ribosomal protein L13 [Bacillus
UniRef100_P70974

Bacillus subtilis

RplM

subtilis]

154
30S ribosomal protein S9 [Bacillus
UniRef100_P21470

Bacillus subtilis

RpsI

subtilis]

155
Hypothetical protein [Bacillus cereus]
UniRef100_Q737T6

Bacillus cereus

YizA

156
Hypothetical protein ybaK [Bacillus
UniRef100_P50862

Bacillus subtilis

YbaK

subtilis]

157
Germination-specific N-
UniRef100_P50864

Bacillus subtilis

CwlD

acetylmuramoyl-L-alanine amidase

[Bacillus subtilis]

158

YbaL

159
Spore germination protein gerD
UniRef100_P16450

Bacillus subtilis

GerD

precursor [Bacillus subtilis]

160
KinB signaling pathway activation
UniRef100_P16449

Bacillus subtilis

KbaA

protein [Bacillus subtilis]

161
Hypothetical protein ybaN precursor
UniRef100_P50865

Bacillus subtilis

YbaN

[Bacillus subtilis]

162
Penicillin-binding protein [Bacillus
UniRef100_O31773

Bacillus subtilis

PbpX

subtilis]

163

164
Hypothetical protein ybaS [Bacillus
UniRef100_P55190

Bacillus subtilis

YbaS

subtilis]

165
Hypothetical protein yxaJ [Bacillus
UniRef100_P42109

Bacillus subtilis

YxaJ

subtilis]

166
Phenazine biosynthetic protein
UniRef100_Q9HHG6

Halobacterium

YfhB

[Halobacterium sp.]

sp.

167
Hypothetical protein ybbC precursor
UniRef100_P40407

Bacillus subtilis

YbbC

[Bacillus subtilis]

168
Hypothetical lipoprotein ybbD precursor
UniRef100_P40406

Bacillus subtilis

YbbD

[Bacillus subtilis]

169
Hypothetical UPF0214 protein ybbE
UniRef100_O05213

Bacillus subtilis

YbbE

precursor [Bacillus subtilis]

170
YbbF protein [Bacillus subtilis]
UniRef100_Q797S1

Bacillus subtilis

YbbF

171
Putative HTH-type transcriptional
UniRef100_Q45581

Bacillus subtilis

YbbH

regulator ybbH [Bacillus subtilis]

172
Hypothetical protein ybbI [Bacillus
UniRef100_Q45582

Bacillus subtilis

YbbI

subtilis]

173
Hypothetical protein ybbK [Bacillus
UniRef100_Q45584

Bacillus subtilis

YbbK

subtilis]

174
Arginase [Bacillus caldovelox]
UniRef100_P53608

Bacillus

RocF

caldovelox

175
RNA polymerase sigma factor sigW
UniRef100_Q45585

Bacillus subtilis

SigW

[Bacillus subtilis]

176
YbbM protein [Bacillus subtilis]
UniRef100_Q45588

Bacillus subtilis

YbbM

177
YbbP protein [Bacillus subtilis]
UniRef100_Q45589

Bacillus subtilis

YbbP

178
YbbR protein [Bacillus subtilis]
UniRef100_O34659

Bacillus subtilis

YbbR

179
YbbT protein [Bacillus subtilis]
UniRef100_O34824

Bacillus subtilis

YbbT

180
Glucosamine--fructose-6-phosphate
UniRef100_P39754
isomerizing
GlmS

aminotransferase [isomerizing] [Bacillus

subtilis]

181

182
Hypothetical protein [Bacillus
UniRef100_Q6HH47

Bacillus thuringiensis

thuringiensis]

183
UPI00003CBF92 UniRef100 entry
UniRef100_UPI00003CBF92

YtrB

184
Transcriptional regulator [Bacillus
UniRef100_Q9KF35

Bacillus

YtrA

halodurans]

halodurans

185

186
YbcL protein [Bacillus subtilis]
UniRef100_O34663

Bacillus subtilis

YbcL

187
BH0186 protein [Bacillus halodurans]
UniRef100_Q9KGB8

Bacillus halodurans

188
ABC transporter [Bacillus halodurans]
UniRef100_Q9KEY6

Bacillus

YvcC

halodurans

189
Hypothetical protein ywbO [Bacillus
UniRef100_P39598

Bacillus subtilis

YwbO

subtilis]

190

191
BH0695 protein [Bacillus halodurans]
UniRef100_Q9KF04

Bacillus halodurans

192
Aminoglycoside 6-adenylyltranserase
UniRef100_Q6V4U6

Enterococcus

AadK

[Enterococcus faecium]

faecium

193
Hypothetical conserved protein
UniRef100_Q8ERX2

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

194
YfnB [Bacillus subtilis]
UniRef100_O06480

Bacillus subtilis

YfnB

195
Hypothetical transport protein ybxG
UniRef100_P54425

Bacillus subtilis

YbxG

[Bacillus subtilis]

196
Mg(2+)/citrate complex secondary
UniRef100_P55069

Bacillus subtilis

CitM

transporter [Bacillus subtilis]

197
YflP protein [Bacillus subtilis]
UniRef100_O34439

Bacillus subtilis

YflP

198

CitT

199
Sensor protein citS [Bacillus subtilis]
UniRef100_O34427

Bacillus subtilis

CitS

200
Transcriptional regulator
UniRef100_Q8RB37

Thermoanaerobacter

YwfK

[Thermoanaerobacter tengcongensis]

tengcongensis

201
Complete genome; segment 8/17
UniRef100_Q7N4W7

Photorhabdus

YwfE

[Photorhabdus luminescens]

luminescens

202
Multidrug resistance protein B [Bacillus
UniRef100_Q81AF0

Bacillus cereus

YqjV

cereus]

203
Sigma-G-dependent sporulation
UniRef100_P54379

Bacillus subtilis

specific SASP protein [Bacillus subtilis]

204
Hypothetical protein ybxH [Bacillus
UniRef100_P54426

Bacillus subtilis

subtilis]

205
YbyB protein [Bacillus subtilis]
UniRef100_O31441

Bacillus subtilis

206
Hypothetical protein yyaL [Bacillus
UniRef100_P37512

Bacillus subtilis

YyaL

subtilis]

207
Hypothetical protein yyaO [Bacillus
UniRef100_P37509

Bacillus subtilis

subtilis]

208
Threonyl-tRNA synthetase 2 [Bacillus
UniRef100_P18256

Bacillus subtilis

ThrZ

subtilis]

209

210

YttB

211
Hypothetical protein [Acinetobacter sp.]
UniRef100_Q6FDN0

Acinetobacter sp.

212
Hypothetical UPF0053 protein yrkA
UniRef100_P54428

Bacillus subtilis

YrkA

[Bacillus subtilis]

213

YdeG

214

YbaJ

215

216

YqeW

217
YubF protein [Bacillus subtilis]
UniRef100_O32082

Bacillus subtilis

218

YbfF

219
Hypothetical protein [Bacteroides
UniRef100_Q64UG6

Bacteroides fragilis

fragilis]

220
Hypothetical transport protein ybhF
UniRef100_O31448

Bacillus subtilis

YbfH

[Bacillus subtilis]

221
YbfI protein [Bacillus subtilis]
UniRef100_O31449

Bacillus subtilis

YbfI

222

223
UPI00003CBC98 UniRef100 entry
UniRef100_UPI00003CBC98

224

225
Oxidoreductase [Lactococcus lactis]
UniRef100_Q9CFC5

Lactococcus lactis

226
Methyl-accepting chemotaxis protein
UniRef100_Q8EST3

Oceanobacillus

YvaQ

[Oceanobacillus iheyensis]

iheyensis

227
D-xylose-binding protein
UniRef100_O68456

Thermoanaerobacter

RbsB

[Thermoanaerobacter ethanolicus]

ethanolicus

228
Hypothetical protein OB0544
UniRef100_Q8ESS4

Oceanobacillus

YomI

[Oceanobacillus iheyensis]

iheyensis

229
Hypothetical protein [Bacillus cereus]
UniRef100_Q81IR4

Bacillus cereus

230
Streptogramin B lactonase
UniRef100_O87275

Staphylococcus cohnii

[Staphylococcus cohnii]

231
Hypothetical protein ycbP [Bacillus
UniRef100_P42248

Bacillus subtilis

YcbP

subtilis]

232
YbgF protein [Bacillus subtilis]
UniRef100_O31462

Bacillus subtilis

YbgF

233
YbgG protein [Bacillus subtilis]
UniRef100_O31463

Bacillus subtilis

YbgG

234

235
PTS system, n-acetylglucosamine-
UniRef100_Q9KF24

Bacillus

NagP

specific enzyme II, ABC component

halodurans

[Bacillus halodurans]

236
UPI00003CBDBC UniRef100 entry
UniRef100_UPI00003CBDBC

Mta

237
Hypothetical protein [Bacillus
UniRef100_Q6HIW0

Bacillus

YdfS

thuringiensis]

thuringiensis

238
Carbonic anhydrase [Methanosarcina
UniRef100_Q8PSJ1

Methanosarcina

YtoA

mazei]

mazei

239
Hypothetical protein yjgA [Bacillus
UniRef100_Q6HHU2

Bacillus

YjgA

thuringiensis]

thuringiensis

240
Amino acid carrier protein [Bacillus
UniRef100_Q6HGU4

Bacillus

YbgH

thuringiensis]

thuringiensis

241
Probable glutaminase ybgJ [Bacillus
UniRef100_O31465

Bacillus subtilis

YbgJ

subtilis]

242
Two-component sensor kinase ycbA
UniRef100_Q81BN8

Bacillus cereus

YcbA

[Bacillus cereus]

243
Hypothetical sensory transduction
UniRef100_P40759

Bacillus subtilis

YcbB

protein ycbB [Bacillus subtilis]

244

YoaA

245

YbxI

246

247
Beta-lactamase precursor (EC 3.5.2.6)
UniRef100_P00808
Contains: Large
PenP

(Penicillinase) [Contains: Large

exopenicillinase;

exopenicillinase; Small

Small

exopenicillinase] [Bacillus licheniformis]

exopenicillinase

248
Alkaline phosphatase D precursor
UniRef100_P42251

Bacillus subtilis

PhoD

[Bacillus subtilis]

249

250
Hypothetical protein ycbT [Bacillus
UniRef100_P42252

Bacillus subtilis

TatCD

subtilis]

251

YcbC

252
Probable aldehyde dehydrogenase
UniRef100_P42236

Bacillus subtilis

YcbD

ycbD [Bacillus subtilis]

253
Probable glucarate transporter [Bacillus
UniRef100_P42237

Bacillus subtilis

YcbE

subtilis]

254
Probable glucarate dehydratase
UniRef100_P42238

Bacillus subtilis

YcbF

[Bacillus subtilis]

255
Hypothetical transcriptional regulator
UniRef100_P42239

Bacillus subtilis

YcbG

ycbG [Bacillus subtilis]

256
Probable D-galactarate dehydratase
UniRef100_P42240

Bacillus subtilis

YcbH

[Bacillus subtilis]

257
Hypothetical sensory transduction
UniRef100_P42244

Bacillus subtilis

YcbL

protein ycbL [Bacillus subtilis]

258
Sensor histidine kinase [Bacillus cereus
UniRef100_Q633Q6

Bacillus cereus ZK

ZK]

259
Hypothetical ABC transporter ATP-
UniRef100_P42246

Bacillus subtilis

YcbN

binding protein ycbN [Bacillus subtilis]

260
Hypothetical protein ycbO [Bacillus
UniRef100_P42247

Bacillus subtilis

YcbO

subtilis]

261

YcbO

262
YfnK [Bacillus subtilis]
UniRef100_O06490

Bacillus subtilis

YetN

263
Hypothetical protein ybdO [Bacillus
UniRef100_O31437

Bacillus subtilis

YbdO

subtilis]

264
Hypothetical protein ycbJ [Bacillus
UniRef100_P42242

Bacillus subtilis

YcbJ

subtilis]

265
Hypothetical protein ywhA [Bacillus
UniRef100_P70993

Bacillus subtilis

YwhA

subtilis]

266

YdaB

267
AII0778 protein [Anabaena sp.]
UniRef100_Q8YYR8

Anabaena sp.

268
BH1298 protein [Bacillus halodurans]
UniRef100_Q9KDB5

Bacillus

YbdN

halodurans

269
BH1299 protein [Bacillus halodurans]
UniRef100_Q9KDB4

Bacillus halodurans

270
Transcriptional activator tipA, putative
UniRef100_Q81XN3

Bacillus

Mta

[Bacillus anthracis]

anthracis

271

272
Transcriptional regulator, PadR family
UniRef100_Q81BZ0

Bacillus cereus

[Bacillus cereus]

273
Hypothetical protein [Bacillus cereus
UniRef100_Q639U7

Bacillus cereus ZK

ZK]

274
Tryptophan RNA-binding attenuator
UniRef100_O31466

Bacillus subtilis

protein-inhibitory protein [Bacillus

subtilis]

275
Hypothetical transport protein ycbK
UniRef100_P42243

Bacillus subtilis

YcbK

[Bacillus subtilis]

276

YczC

277
Hypothetical protein yccF [Bacillus
UniRef100_O34478

Bacillus subtilis

YccF

subtilis]

278
SpaF [Bacillus subtilis]
UniRef100_Q45404

Bacillus subtilis

YhcH

279
SpaE [Bacillus subtilis]
UniRef100_O52853

Bacillus subtilis

280
Putative SpaG [Bacillus subtilis]
UniRef100_Q93GG9

Bacillus subtilis

281
Subtilin biosynthesis regulatory protein
UniRef100_P33112

Bacillus subtilis

YycF

spaR [Bacillus subtilis]

282
Putative histidine kinase [Bacillus
UniRef100_Q93GG7

Bacillus subtilis

ResE

subtilis]

283
UPI00003CA401 UniRef100 entry
UniRef100_UPI00003CA401

YtlI

284
YusQ protein [Bacillus subtilis]
UniRef100_O32183

Bacillus subtilis

YusQ

285
Complete genome; segment 6/17
UniRef100_Q7N6K9

Photorhabdus

YusR

[Photorhabdus luminescens]

luminescens

286
Methyltransferase [Bacillus
UniRef100_Q6HK82

Bacillus thuringiensis

thuringiensis]

287
Hypothetical lipoprotein ycdA precursor
UniRef100_O34538

Bacillus subtilis

YcdA

[Bacillus subtilis]

288
UPI00003CB481 UniRef100 entry
UniRef100_UPI00003CB481

YcgA

289
Hypothetical conserved protein
UniRef100_Q8ESQ2

Oceanobacillus

YtnL

[Oceanobacillus iheyensis]

iheyensis

290
Hypothetical conserved protein
UniRef100_Q8ET83

Oceanobacillus

YvbK

[Oceanobacillus iheyensis]

iheyensis

291

GatA

292
Oligoendopeptidase F, putative
UniRef100_Q81JJ8

Bacillus

YjbG

[Bacillus anthracis]

anthracis

293

294
UPI00003CC42D UniRef100 entry
UniRef100_UPI00003CC42D

PbpX

295
Hypothetical protein ysfD [Bacillus
UniRef100_P94534

Bacillus subtilis

YsfD

subtilis]

296
Hypothetical protein ysfC [Bacillus
UniRef100_P94535

Bacillus subtilis

YsfC

subtilis]

297
PTS system, cellobiose-specific
UniRef100_Q9KEE3

Bacillus halodurans

enzyme II, B component [Bacillus

halodurans]

298
PTS system, cellobiose-specific
UniRef100_Q9KEE2

Bacillus

LicA

enzyme II, A component [Bacillus

halodurans

halodurans]

299
PTS system, cellobiose-specific
UniRef100_Q8EP43

Oceanobacillus

YwbA

enzyme II, C component

iheyensis

[Oceanobacillus iheyensis]

300
6-phospho-beta-glucosidase [Bacillus
UniRef100_Q9KEE0

Bacillus

LicH

halodurans]

halodurans

301
Transcriptional regulator [Bacillus
UniRef100_Q9KED8

Bacillus

YbgA

halodurans]

halodurans

302

303

YvbX

304
Chitinase precursor [Streptomyces
UniRef100_Q9L3E8

Streptomyces olivaceoviridis

olivaceoviridis]

305
Extracellular metalloprotease precursor
UniRef100_P39790

Bacillus subtilis

Mpr

[Bacillus subtilis]

306
Glucose 1-dehydrogenase II [Bacillus
UniRef100_P80869

Bacillus subtilis

YcdF

subtilis]

307
Hypothetical protein OB0244
UniRef100_Q8ETL6

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

308
Hypothetical protein ycdC [Bacillus
UniRef100_O34772

Bacillus subtilis

YcdC

subtilis]

309

310
Cell wall hydrolase cwlJ [Bacillus
UniRef100_P42249

Bacillus subtilis

CwlJ

subtilis]

311
Hypothetical protein yceB [Bacillus
UniRef100_O34504

Bacillus subtilis

YceB

subtilis]

312
Hypothetical protein yvcE [Bacillus
UniRef100_P40767

Bacillus subtilis

YvcE

subtilis]

313
ABC transporter, permease [Bacillus
UniRef100_Q637N2

Bacillus cereus

YfiM

cereus ZK]

ZK

314
ABC transporter, ATP-binding protein
UniRef100_Q733P8

Bacillus cereus

YfiL

[Bacillus cereus]

315
UPI00003CC482 UniRef100 entry
UniRef100_UPI00003CC482

YsiA

316
Hypothetical protein OB3113
UniRef100_Q8ELV2

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

317
BH3953 protein [Bacillus halodurans]
UniRef100_Q9K5Y3

Bacillus halodurans

318
BH3951 protein [Bacillus halodurans]
UniRef100_Q9K5Y4

Bacillus

PadR

halodurans

319
Stress response protein SCP2 [Bacillus
UniRef100_P81100

Bacillus subtilis

YceC

subtilis]

320
General stress protein 16U [Bacillus
UniRef100_P80875

Bacillus subtilis

YceD

subtilis]

321
Hypothetical protein yceE [Bacillus
UniRef100_O34384

Bacillus subtilis

YceE

subtilis]

322
Hypothetical protein yceF [Bacillus
UniRef100_O34447

Bacillus subtilis

YceF

subtilis]

323
Hypothetical protein [Bacillus cereus]
UniRef100_Q72YD0

Bacillus cereus

324
YceG [Bacillus subtilis]
UniRef100_O34809

Bacillus subtilis

YceG

325
Hypothetical protein yceH [Bacillus
UniRef100_O34833

Bacillus subtilis

YceH

subtilis]

326
UPI00003CB694 UniRef100 entry
UniRef100_UPI00003CB694

CcdA

327
Hypothetical conserved protein
UniRef100_Q8ELB4

Oceanobacillus

YdiL

[Oceanobacillus iheyensis]

iheyensis

328

Mta

329
Nitrate transporter [Bacillus subtilis]
UniRef100_P42432

Bacillus subtilis

NasA

330
L-lactate dehydrogenase [Bacillus
UniRef100_P13714

Bacillus subtilis

Ldh

subtilis]

331
L-lactate permease [Bacillus subtilis]
UniRef100_P55910

Bacillus subtilis

LctP

332
YcgF protein [Bacillus subtilis]
UniRef100_P94381

Bacillus subtilis

YcgF

333
Homologue of aromatic amino acids
UniRef100_P94383

Bacillus subtilis

YcgH

transport protein of E. coli [Bacillus

subtilis]

334
NH(3)-dependent NAD(+) synthetase
UniRef100_P08164

Bacillus subtilis

NadE

[Bacillus subtilis]

335
Shikimate kinase [Bacillus subtilis]
UniRef100_P37944

Bacillus subtilis

AroK

336
YcgL protein [Bacillus subtilis]
UniRef100_P94389

Bacillus subtilis

YcgL

337
Proline dehydrogenase [Bacillus
UniRef100_Q8RL79

Bacillus subtilis

YcgM

subtilis]

338
1-pyrroline-5-carboxylate
UniRef100_P94391

Bacillus subtilis

YcgN

dehydrogenase 2 [Bacillus subtilis]

339
Homologue of proline permease of E. coli
UniRef100_P94392

Bacillus subtilis

YcgO

[Bacillus subtilis]

340
Hypothetical protein ycgP [Bacillus
UniRef100_P94393

Bacillus subtilis

YcgP

subtilis]

341
YcgQ protein [Bacillus subtilis]
UniRef100_P94394

Bacillus subtilis

YcgQ

342

YcgR

343
Cephalosporin-C deacetylase [Bacillus
UniRef100_Q59233

Bacillus subtilis

Cah

subtilis]

344
Transcriptional regulator [Bacillus
UniRef100_Q9KF41

Bacillus

YdgG

halodurans]

halodurans

345

346

347
Probable amino-acid ABC transporter
UniRef100_P42399

Bacillus subtilis

YckA

permease protein yckA [Bacillus

subtilis]

348
Probable ABC transporter extracellular
UniRef100_P42400

Bacillus subtilis

YckB

binding protein yckB precursor [Bacillus

subtilis]

349
NAD(P)H dehydrogenase (Quinone);
UniRef100_Q63FG6

Bacillus cereus

YwrO

possible modulator of drug activity B

ZK

[Bacillus cereus ZK]

350
BH0315 protein [Bacillus halodurans]
UniRef100_Q9KG01

Bacillus halodurans

351
Probable beta-glucosidase [Bacillus
UniRef100_P42403

Bacillus subtilis

YckE

subtilis]

352
Nin [Bacillus amyloliquefaciens]
UniRef100_Q70KK3

Bacillus

Nin

amyloliquefaciens

353
DNA-entry nuclease [Bacillus subtilis]
UniRef100_P12667

Bacillus subtilis

NucA

354

YbdM

355
Methyl-accepting chemotaxis protein
UniRef100_P39209

Bacillus subtilis

TlpC

tlpC [Bacillus subtilis]

356
Hypothetical protein yqcG [Bacillus
UniRef100_P45942

Bacillus subtilis

YqcG

subtilis]

357
Hydantoin utilization protein A
UniRef100_Q7WCS3

Bordetella bronchiseptica

[Bordetella bronchiseptica]

358
Hydantoin utilization protein B
UniRef100_Q987J6

Rhizobium loti

[Rhizobium loti]

359
UPI000027D233 UniRef100 entry
UniRef100_UPI000027D233

YvfK

360
358aa long hypothetical transporter
UniRef100_Q9YB65

Aeropyrum

MsmX

ATP-binding protein [Aeropyrum pernix]

pernix

361
AttA2-like ABC transporter, permease
UniRef100_Q92ZH0

Rhizobium

YesP

protein [Rhizobium meliloti]

meliloti

362

YurM

363

SrfAA

364

SrfAB

365

SrfAC

366

SrfAD

367

YcxC

368

YcxD

369
4′-phosphopantetheinyl transferase sfp
UniRef100_P39135

Bacillus subtilis

Sfp

[Bacillus subtilis]

370
Predicted esterase of alpha/beta
UniRef100_Q97HP7

Clostridium

YbbA

hydrolase superfamily, YBBA B. subtilis

acetobutylicum

ortholog [Clostridium acetobutylicum]

371
Transcriptional regulator [Clostridium
UniRef100_Q97LX8

Clostridium

YdeE

acetobutylicum]

acetobutylicum

372
YczE [Bacillus subtilis]
UniRef100_Q9F4F8

Bacillus subtilis

YczE

373
Hypothetical protein [Symbiobacterium
UniRef100_Q67MZ7

Symbiobacterium

thermophilum]

thermophilum

374

YfiL

375

376
Hypothetical protein [Symbiobacterium
UniRef100_Q67MZ9

Symbiobacterium

thermophilum]

thermophilum

377

378

379
Methyl-accepting chemotaxis protein
UniRef100_Q6HJV7

Bacillus

TlpB

[Bacillus thuringiensis]

thuringiensis

380
YckI [Bacillus subtilis]
UniRef100_Q9F4F9

Bacillus subtilis

YckI

381
YckJ [Bacillus subtilis]
UniRef100_Q9F4G0

Bacillus subtilis

YckJ

382

YckK

383

RocR

384
Ornithine aminotransferase [Bacillus
UniRef100_P38021

Bacillus subtilis

RocD

subtilis]

385
Amino-acid permease rocE [Bacillus
UniRef100_P39137

Bacillus subtilis

RocE

subtilis]

386
Arginase [Bacillus subtilis]
UniRef100_P39138

Bacillus subtilis

RocF

387
Homologue of als operon regulatory
UniRef100_P94403

Bacillus subtilis

YclA

protein AlsR of B. subtilis [Bacillus

subtilis]

388
Probable aromatic acid decarboxylase
UniRef100_P94404

Bacillus subtilis

YclB

[Bacillus subtilis]

389
Hypothetical protein yclC [Bacillus
UniRef100_P94405

Bacillus subtilis

YclC

subtilis]

390
YclD protein [Bacillus subtilis]
UniRef100_P94406

Bacillus subtilis

YclD

391

392

393

394
Hypothetical protein [Bacillus
UniRef100_Q6HMQ9

Bacillus thuringiensis

thuringiensis]

395
Hypothetical protein OB2810
UniRef100_Q8EMN2

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

396

397

YxiD

398

399

YxiB

400
Sugar ABC transporter [Bacillus
UniRef100_Q9K7B8

Bacillus

RbsB

halodurans]

halodurans

401
Two-component sensor histidine kinase
UniRef100_Q9K7B9

Bacillus

YesM

[Bacillus halodurans]

halodurans

402

YesN

403
Multiple sugar transport system
UniRef100_Q9K7C2

Bacillus

RbsB

[Bacillus halodurans]

halodurans

404
L-arabinose transport ATP-binding
UniRef100_Q9S472

Bacillus

RbsA

protein araG [Bacillus

stearothermophilus

stearothermophilus]

405
L-arabinose membrane permease
UniRef100_Q9S471

Bacillus

RbsC

[Bacillus stearothermophilus]

stearothermophilus

406
Transporter [Bacillus halodurans]
UniRef100_Q9K9F3

Bacillus

YbfB

halodurans

407
YbfA protein [Bacillus subtilis]
UniRef100_O31443

Bacillus subtilis

YbfA

408
Beta-D-galactosidase [Bacillus
UniRef100_Q45093

Bacillus circulans

LacA

circulans]

409

Phy

410
Hypothetical transporter yclF [Bacillus
UniRef100_P94408

Bacillus subtilis

YclF

subtilis]

411
YclG protein [Bacillus subtilis]
UniRef100_P94409

Bacillus subtilis

YclG

412

413

GerKA

414
Spore germination protein KC precursor
UniRef100_P49941

Bacillus subtilis

GerKC

[Bacillus subtilis]

415
Spore germination protein KB [Bacillus
UniRef100_P49940

Bacillus subtilis

GerKB

subtilis]

416

Mta

417
Hypothetical protein yclH [Bacillus
UniRef100_P94411

Bacillus subtilis

YclH

subtilis]

418
Hypothetical protein yclI [Bacillus
UniRef100_P94412

Bacillus subtilis

YclI

subtilis]

419
Hypothetical sensory transduction
UniRef100_P94413

Bacillus subtilis

YclJ

protein yclJ [Bacillus subtilis]

420
Hypothetical sensor-like histidine
UniRef100_P94414

Bacillus subtilis

YclK

kinase yclK [Bacillus subtilis]

421
Methyl-accepting chemotaxis protein
UniRef100_Q9K632

Bacillus

TlpA

[Bacillus halodurans]

halodurans

422
Probable aspartokinase [Bacillus
UniRef100_P94417

Bacillus subtilis

YclM

subtilis]

423
Homologue of ferric anguibactin
UniRef100_P94418

Bacillus subtilis

YclN

transport system permerase protein

FatD of V. anguillarum [Bacillus subtilis]

424
Homologue of ferric anguibactin
UniRef100_P94419

Bacillus subtilis

YclO

transport system permerase protein

FatC of V. anguillarum [Bacillus subtilis]

425
Homologue of iron dicitrate transport
UniRef100_P94420

Bacillus subtilis

YclP

ATP-binding protein FecE of E. coli

[Bacillus subtilis]

426
Ferric anguibactin-binding protein
UniRef100_P94421

Bacillus subtilis

YclQ

precusor FatB of V. anguillarum

[Bacillus subtilis]

427
Homologue of multidrug resistance
UniRef100_P94422

Bacillus subtilis

YcnB

protein B, EmrB, of E. coli [Bacillus

subtilis]

428
YcnC protein [Bacillus subtilis]
UniRef100_P94423

Bacillus subtilis

YcnC

429
Hypothetical protein [Bacillus cereus
UniRef100_Q636R0

Bacillus cereus

YqiQ

ZK]

ZK

430
Hypothetical oxidoreductase ycnD
UniRef100_P94424

Bacillus subtilis

YcnD

[Bacillus subtilis]

431
Hypothetical protein ycnE [Bacillus
UniRef100_P94425

Bacillus subtilis

subtilis]

432
YczG protein [Bacillus subtilis]
UniRef100_O31480

Bacillus subtilis

433
Homologue of regulatory protein MocR
UniRef100_P94426

Bacillus subtilis

GabR

of R. meliloti [Bacillus subtilis]

434
Probable 4-aminobutyrate
UniRef100_P94427

Bacillus subtilis

GabT

aminotransferase (EC 2.6.1.19) ((S)-3-

amino-2-methylpropionate

transaminase) [Bacillus subtilis]

435
Cationic amino acid transporter
UniRef100_Q8ESX7

Oceanobacillus

YhdG

[Oceanobacillus iheyensis]

iheyensis

436
Homologue of succinate semialdehyde
UniRef100_P94428

Bacillus subtilis

GabD

dehydrogenase GabD of E. coli

[Bacillus subtilis]

437

YwfM

438

439
YcnI protein [Bacillus subtilis]
UniRef100_P94431

Bacillus subtilis

YcnI

440
Homologue of copper export protein
UniRef100_P94432

Bacillus subtilis

YcnJ

PcoD of E. coli [Bacillus subtilis]

441
YcnK protein [Bacillus subtilis]
UniRef100_P94433

Bacillus subtilis

YcnK

442
Assimilatory nitrate reductase electron
UniRef100_P42433

Bacillus subtilis

NasB

transfer subunit [Bacillus subtilis]

443
Assimilatory nitrate reductase catalytic
UniRef100_P42434

Bacillus subtilis

NasC

subunit [Bacillus subtilis]

444
Nitrite reductase [NAD(P)H] [Bacillus
UniRef100_P42435
NAD(P)H
NasD

subtilis]

445
Assimilatory nitrite reductase
UniRef100_P42436
NAD(P)H
NasE

[NAD(P)H] small subunit [Bacillus

subtilis]

446
Uroporphyrin-III C-methyltransferase
UniRef100_P42437

Bacillus subtilis

NasF

[Bacillus subtilis]

447
Hypothetical transcriptional regulator
UniRef100_O05494

Bacillus subtilis

YdhC

ydhC [Bacillus subtilis]

448
Sodium-dependent transporter
UniRef100_Q8ENE3

Oceanobacillus

YflS

[Oceanobacillus iheyensis]

iheyensis

449
Hypothetical protein ycsD [Bacillus
UniRef100_P42961

Bacillus subtilis

subtilis]

450
UPI00003CC424 UniRef100 entry
UniRef100_UPI00003CC424

451
Possible transcriptional antiterminator,
UniRef100_Q63A16

Bacillus cereus

MtlR

bglG family [Bacillus cereus ZK]

ZK

452
Putative sugar-specific PTS component
UniRef100_Q7X1N9

Lactococcus raffinolactis

EIIB [Lactococcus raffinolactis]

453
SgaT protein [Mannheimia
UniRef100_Q65WA2

Mannheimia

succiniciproducens MBEL55E]

succiniciproducens MBEL55E

454
Transketolase, N-terminal subunit
UniRef100_Q8E202

Streptococcus

Tkt

[Streptococcus agalactiae]

agalactiae

455
Putative transketolase [Salmonella
UniRef100_Q8ZND5

Salmonella

Dxs

typhimurium]

typhimurium

456
Hypothetical protein ycsE [Bacillus
UniRef100_P42962

Bacillus subtilis

YcsE

subtilis]

457
Hypothetical UPF0271 protein ycsF
UniRef100_P42963

Bacillus subtilis

YcsF

[Bacillus subtilis]

458
Hypothetical protein ycsG [Bacillus
UniRef100_P42964

Bacillus subtilis

YcsG

subtilis]

459
Hypothetical UPF0317 protein ycsI
UniRef100_P42966

Bacillus subtilis

YcsI

[Bacillus subtilis]

460
Kinase A inhibitor [Bacillus subtilis]
UniRef100_P60495

Bacillus subtilis

KipI

461
KipI antagonist [Bacillus subtilis]
UniRef100_Q7WY77

Bacillus subtilis

KipA

462
HTH-type transcriptional regulator kipR
UniRef100_P42968

Bacillus subtilis

KipR

[Bacillus subtilis]

463
Hypothetical protein ycsK [Bacillus
UniRef100_P42969

Bacillus subtilis

YcsK

subtilis]

464
PTS system, mannitol-specific IIABC
UniRef100_P42956

Bacillus subtilis

MtlA

component [Bacillus subtilis]

465
Mannitol-1-phosphate 5-
UniRef100_P42957

Bacillus subtilis

MtlD

dehydrogenase [Bacillus subtilis]

466
YdaA protein [Bacillus subtilis]
UniRef100_P96574

Bacillus subtilis

MtlR

467
General stress protein 39 [Bacillus
UniRef100_P80873

Bacillus subtilis

YdaD

subtilis]

468
Hypothetical protein ydaE [Bacillus
UniRef100_P96578

Bacillus subtilis

YdaE

subtilis]

469
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81U55

Bacillus anthracis

470
General stress protein 26 [Bacillus
UniRef100_P80238

Bacillus subtilis

YdaG

subtilis]

471
YdaH protein [Bacillus subtilis]
UniRef100_P96581

Bacillus subtilis

YdaH

472
Lin0463 protein [Listeria innocua]
UniRef100_Q92EJ6

Listeria innocua

YvhJ

473
YdzA protein [Bacillus subtilis]
UniRef100_O31485

Bacillus subtilis

474
BH0424 protein [Bacillus halodurans]
UniRef100_Q9KFQ4

Bacillus halodurans

475
HTH-type transcriptional regulator lrpC
UniRef100_P96582

Bacillus subtilis

LrpC

[Bacillus subtilis]

476
PROBABLE DNA TOPOISOMERASE
UniRef100_P96583

Bacillus subtilis

TopB

III [Bacillus subtilis]

477

478

479

480

YdaO

481

YdaO

482
YdaP protein [Bacillus subtilis]
UniRef100_P96591

Bacillus subtilis

YdaP

483

484
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069

485
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012

anthracis str. A2012]

486

487
Similar to ribosomal-protein-serine N-
UniRef100_Q99WN5

Staphylococcus

YdaF

acetyltransferase [Staphylococcus

aureus

aureus]

488
Manganese transport protein mntH
UniRef100_P96593

Bacillus subtilis

MntH

[Bacillus subtilis]

489

490

AnsB

491

YojK

492
YdaT protein [Bacillus subtilis]
UniRef100_P96595

Bacillus subtilis

YdaT

493
Hypothetical protein ydbA [Bacillus
UniRef100_P96596

Bacillus subtilis

YdbA

subtilis]

494
Na+/H+ antiporter NhaC [Bacillus
UniRef100_Q81FX8

Bacillus cereus

NhaC

cereus]

495
YdbB protein [Bacillus subtilis]
UniRef100_P96597

Bacillus subtilis

YdbB

496
Glucose starvation-inducible protein B
UniRef100_P26907

Bacillus subtilis

GsiB

[Bacillus subtilis]

497
Hypothetical UPF0118 protein ydbI
UniRef100_P96604

Bacillus subtilis

YdbI

[Bacillus subtilis]

498

GltT

499
YdbJ protein [Bacillus subtilis]
UniRef100_P96605

Bacillus subtilis

YdbJ

500
YdbK protein [Bacillus subtilis]
UniRef100_P96606

Bacillus subtilis

YdbK

501
Hypothetical protein ydbL [Bacillus
UniRef100_P96607

Bacillus subtilis

YdbL

subtilis]

502
YdbM protein [Bacillus subtilis]
UniRef100_P96608

Bacillus subtilis

YdbM

503

504

505
YdbP protein [Bacillus subtilis]
UniRef100_P96611

Bacillus subtilis

YdbP

506
D-alanine--D-alanine ligase [Bacillus
UniRef100_P96612

Bacillus subtilis

Ddl

subtilis]

507

MurF

508
Esterase [Oceanobacillus iheyensis]
UniRef100_Q8ESM0

Oceanobacillus

YvaK

iheyensis

509
YdbR protein [Bacillus subtilis]
UniRef100_P96614

Bacillus subtilis

YdbR

510
YdbS protein [Bacillus subtilis]
UniRef100_P96615

Bacillus subtilis

YdbS

511
YdbT protein [Bacillus subtilis]
UniRef100_P96616

Bacillus subtilis

YdbT

512
YdcA protein [Bacillus subtilis]
UniRef100_P96617

Bacillus subtilis

YdcA

513

YdcC

514

Alr

515

516
YdcE protein [Bacillus subtilis]
UniRef100_P96622

Bacillus subtilis

YdcE

517

RsbR

518

RsbS

519

RsbT

520

RsbU

521

RsbV

522

RsbW

523

SigB

524

RsbX

525
YdcI protein [Bacillus subtilis]
UniRef100_O31489

Bacillus subtilis

YdcI

526
Transcriptional regulator, TetR family
UniRef100_Q6HGY5

Bacillus

YxbF

[Bacillus thuringiensis]

thuringiensis

527
Lin1189 protein [Listeria innocua]
UniRef100_Q92CI2

Listeria innocua

YdgH

528

529
Protein sprT-like [Bacillus subtilis]
UniRef100_P96628

Bacillus subtilis

YdcK

530
Possible transporter, EamA family
UniRef100_Q638K5

Bacillus cereus ZK

[Bacillus cereus ZK]

531

532
Hypothetical protein ORF00034
UniRef100_O87235

Lactococcus lactis

[Lactococcus lactis]

533
Delta5 acyl-lipid desaturase [Bacillus
UniRef100_Q81C02

Bacillus cereus

Des

cereus]

534
Cold shock protein cspC [Bacillus
UniRef100_P39158

Bacillus subtilis

subtilis]

535

YogA

536
Membrane protein, putative [Bacillus
UniRef100_Q734Y0

Bacillus cereus

YyaS

cereus]

537
Transcriptional regulator, MarR family
UniRef100_Q734X9

Bacillus cereus

YybA

[Bacillus cereus]

538
Acetyltransferase, GNAT family
UniRef100_Q734X8

Bacillus cereus

PaiA

[Bacillus cereus]

539
Protease synthase and sporulation
UniRef100_Q734X7

Bacillus cereus

PaiB

negative regulatory protein PAI 2

[Bacillus cereus]

540
BH0654 protein [Bacillus halodurans]
UniRef100_Q9KF33

Bacillus

RocF

halodurans

541

542
YdeO protein [Bacillus subtilis]
UniRef100_P96672

Bacillus subtilis

YdeO

543
Transporter, LysE family [Bacillus
UniRef100_Q81D17

Bacillus cereus

YrhP

cereus]

544

YwqM

545

546
Permease, putative [Bacillus anthracis]
UniRef100_Q81QY7

Bacillus

YvqJ

anthracis

547
Putative cyclase [Rhodopseudomonas
UniRef100_Q6N497

Rhodopseudomonas palustris

palustris]

548
Hypothetical transport protein ydgF
UniRef100_P96704

Bacillus subtilis

YdgF

[Bacillus subtilis]

549

550

YknW

551
RNA polymerase sigma factor sigV
UniRef100_O05404

Bacillus subtilis

SigV

[Bacillus subtilis]

552
Putative anti-SigV factor [Bacillus
UniRef100_O05403

Bacillus subtilis

YrhM

subtilis]

553
Hypothetical protein yrhL [Bacillus
UniRef100_O05402

Bacillus subtilis

YrhL

subtilis]

554

YdgK

555

YwpD

556

LytT

557
Collagen adhesion protein [Bacillus
UniRef100_Q630P2

Bacillus cereus ZK

cereus ZK]

558
Lin0929 protein [Listeria innocua]
UniRef100_Q92D88

Listeria innocua

559

560
Metabolite transport protein [Bacillus
UniRef100_O34718

Bacillus subtilis

YdjK

subtilis]

561
Thiamine-monophosphate kinase
UniRef100_O05514

Bacillus subtilis

ThiL

[Bacillus subtilis]

562
Hypothetical UPF0079 protein ydiB
UniRef100_O05515

Bacillus subtilis

YdiB

[Bacillus subtilis]

563
YdiC protein [Bacillus subtilis]
UniRef100_O05516

Bacillus subtilis

YdiC

564
YdiD protein [Bacillus subtilis]
UniRef100_O05517

Bacillus subtilis

YdiD

565
Probable O-sialoglycoprotein
UniRef100_O05518

Bacillus subtilis

Gcp

endopeptidase [Bacillus subtilis]

566

YdiF

567

YdiF

568
Molybdenum cofactor biosynthesis
UniRef100_O05520

Bacillus subtilis

YdiG

protein C [Bacillus subtilis]

569
Redox-sensing transcriptional repressor
UniRef100_O05521

Bacillus subtilis

YdiH

rex [Bacillus subtilis]

570
YdiI [Bacillus halodurans]
UniRef100_Q9Z9P5

Bacillus halodurans

571

TatCY

572
Hypothetical lipoprotein ydiK precursor
UniRef100_O05524

Bacillus subtilis

[Bacillus subtilis]

573

YdiL

574
10 kDa chaperonin [Bacillus subtilis]
UniRef100_P28599

Bacillus subtilis

575
60 kDa chaperonin [Bacillus subtilis]
UniRef100_P28598

Bacillus subtilis

GroEL

576

577

578
Hypothetical protein yolD
UniRef100_O64030
Bacteriophage SPBc2

[Bacteriophage SPBc2]

579

580

581
YoaR [Bacillus subtilis]
UniRef100_O34611

Bacillus subtilis

YoaR

582
Hypothetical protein yfmQ [Bacillus
UniRef100_O06475

Bacillus subtilis

YfmQ

subtilis]

583

584
YoqW protein [Bacteriophage SPBc2]
UniRef100_O64131
Bacteriophage
YoqW

SPBc2

585
Lin2076 protein [Listeria innocua]
UniRef100_Q92A46

Listeria innocua

YerO

586
PEP synthase [Bacillus subtilis]
UniRef100_O34309

Bacillus subtilis

Pps

587
Hypothetical protein yoaF [Bacillus
UniRef100_O31829

Bacillus subtilis

subtilis]

588
Hypothetical protein [Staphylococcus
UniRef100_Q6GK76

Staphylococcus aureus

aureus]

589
Short-chain dehydrodenase
UniRef100_Q97LM1

Clostridium

DltE

[Clostridium acetobutylicum]

acetobutylicum

590
Type B carboxylesterase [Bacillus sp.
UniRef100_Q9L378

Bacillus sp. BP-7
PnbA

BP-7]

591
Inositol transport protein
UniRef100_Q8ESX2

Oceanobacillus

IolF

[Oceanobacillus iheyensis]

iheyensis

592
Phage shock protein A homolog
UniRef100_P54617

Bacillus subtilis

PspA

[Bacillus subtilis]

593
YdjG protein [Bacillus subtilis]
UniRef100_O34434

Bacillus subtilis

YdjG

594
YdjH protein [Bacillus subtilis]
UniRef100_O35004

Bacillus subtilis

YdjH

595
YdjI protein [Bacillus subtilis]
UniRef100_O34789

Bacillus subtilis

YdjI

596
Putative oxidoreductase
UniRef100_Q67S08

Symbiobacterium

YtmO

[Symbiobacterium thermophilum]

thermophilum

597
YrhO [Bacillus subtilis]
UniRef100_O05405

Bacillus subtilis

YrhO

598

YrhP

599
Helix-turn-helix domain protein [Bacillus
UniRef100_Q73C00

Bacillus cereus

cereus]

600
Stage V sporulation protein E [Bacillus
UniRef100_Q9K7T4

Bacillus

SpoVE

halodurans]

halodurans

601
Stage V sporulation protein E [Bacillus
UniRef100_Q9K7T3

Bacillus

FtsW

halodurans]

halodurans

602

603
Hypothetical protein [Bacillus cereus]
UniRef100_Q72Z89

Bacillus cereus

604
BH1889 protein [Bacillus halodurans]
UniRef100_Q9KBN6

Bacillus

YobV

halodurans

605

YjeA

606

YjeA

607

TreA

608
Putative HTH-type transcriptional
UniRef100_O06987

Bacillus subtilis

YvdE

regulator yvdE [Bacillus subtilis]

609
Hypothetical protein yvdF [Bacillus
UniRef100_O06988

Bacillus subtilis

YvdF

subtilis]

610
Hypothetical protein yvdG [Bacillus
UniRef100_O06989

Bacillus subtilis

YvdG

subtilis]

611
Hypothetical protein yvdH [Bacillus
UniRef100_O06990

Bacillus subtilis

YvdH

subtilis]

612
Hypothetical protein yvdI [Bacillus
UniRef100_O06991

Bacillus subtilis

YvdI

subtilis]

613
Hypothetical protein yvdJ [Bacillus
UniRef100_O06992

Bacillus subtilis

YvdJ

subtilis]

614
Hypothetical glycosyl hydrolase yvdK
UniRef100_O06993

Bacillus subtilis

YvdK

[Bacillus subtilis]

615
Oligo-1,6-glucosidase [Bacillus subtilis]
UniRef100_O06994

Bacillus subtilis

MalL

616
Putative beta-phosphoglucomutase
UniRef100_O06995

Bacillus subtilis

PgcM

[Bacillus subtilis]

617
Tyrosyl-tRNA synthetase 2 [Bacillus
UniRef100_P25151

Bacillus subtilis

TyrZ

subtilis]

618
Putative HTH-type transcriptional
UniRef100_P25150

Bacillus subtilis

YwaE

regulator ywaE [Bacillus subtilis]

619

620
Hypothetical protein SE2399
UniRef100_Q8CQM7

Staphylococcus epidermidis

[Staphylococcus epidermidis]

621
Hypothetical protein ydjM [Bacillus
UniRef100_P40775

Bacillus subtilis

YdjM

subtilis]

622
YdjN protein [Bacillus subtilis]
UniRef100_O34353

Bacillus subtilis

YdjN

623
Hypothetical protein [Bacillus cereus]
UniRef100_Q81AT6

Bacillus cereus

YeaA

624

625
Signal peptidase I [Bacillus cereus]
UniRef100_Q73C25

Bacillus cereus

SipS

626

627
Hypothetical protein yhfK [Bacillus
UniRef100_O07609

Bacillus subtilis

YhfK

subtilis]

628
Transcriptional regulator [Bacillus
UniRef100_Q9K766

Bacillus

YdeE

halodurans]

halodurans

629
Spore coat protein A [Bacillus subtilis]
UniRef100_P07788

Bacillus subtilis

CotA

630

YkrP

631
Extracellular protein [Lactobacillus
UniRef100_Q88T27

Lactobacillus

YcdA

plantarum]

plantarum

632
Hypothetical UPF0018 protein yeaB
UniRef100_P46348

Bacillus subtilis

YeaB

[Bacillus subtilis]

633
YeaC [Bacillus subtilis]
UniRef100_P94474

Bacillus subtilis

YeaC

634
Hypothetical protein [Bacillus cereus]
UniRef100_Q739D8

Bacillus cereus

YeaD

635
YebA [Bacillus subtilis]
UniRef100_P94476

Bacillus subtilis

YebA

636
GMP synthase [glutamine-hydrolyzing]
UniRef100_P29727
glutamine-
GuaA

[Bacillus subtilis]

hydrolyzing

637
Hypoxanthine/guanine permease
UniRef100_O34987

Bacillus subtilis

PbuG

[Bacillus subtilis]

638

YebC

639
Hypothetical UPF0316 protein yebE
UniRef100_O34624

Bacillus subtilis

YebE

[Bacillus subtilis]

640
YebG protein [Bacillus subtilis]
UniRef100_O34700

Bacillus subtilis

641
Phosphoribosylaminoimidazole
UniRef100_P12044

Bacillus subtilis

PurE

carboxylase catalytic subunit [Bacillus

subtilis]

642

PurK

643
Adenylosuccinate lyase [Bacillus
UniRef100_P12047

Bacillus subtilis

PurB

subtilis]

644
Phosphoribosylaminoimidazole-
UniRef100_P12046

Bacillus subtilis

PurC

succinocarboxamide synthase [Bacillus

subtilis]

645
Hypothetical UPF0062 protein yexA
UniRef100_P12049

Bacillus subtilis

[Bacillus subtilis]

646
Phosphoribosylformylglycinamidine
UniRef100_P12041

Bacillus subtilis

PurQ

synthase I [Bacillus subtilis]

647
Phosphoribosylformylglycinamidine
UniRef100_P12042

Bacillus subtilis

PurL

synthase II [Bacillus subtilis]

648
Amidophosphoribosyltransferase
UniRef100_P00497

Bacillus subtilis

PurF

precursor [Bacillus subtilis]

649

PurM

650
Phosphoribosylglycinamide
UniRef100_P12040

Bacillus subtilis

PurN

formyltransferase [Bacillus subtilis]

651
Bifunctional purine biosynthesis protein
UniRef100_P12048
Includes:
PurH

purH [Includes:

Phosphoribosylaminoimidazole-

Phosphoribosylaminoimidazolecarboxamide

carboxamide formyltransferase

formyltransferase (EC 2.1.2.3)

(EC 2.1.2.3)

(AICAR transformylase); IMP

(AICAR

cyclohydrolase (EC 3.5.4.10)

transformylase);

(Inosinicase) (IMP synthetase) (ATIC)]

IMP

[Bacillus subtilis]

cyclohydrolase

(EC 3.5.4.10)

(Inosinicase)

(IMP synthetase)

(ATIC)

652
Phosphoribosylamine--glycine ligase
UniRef100_P12039

Bacillus subtilis

PurD

[Bacillus subtilis]

653

YxbF

654
Putative cytochrome P450 yjiB [Bacillus
UniRef100_O34374

Bacillus subtilis

YjiB

subtilis]

655

656
Hypothetical lipoprotein yybP precursor
UniRef100_P37488

Bacillus subtilis

YybP

[Bacillus subtilis]

657
Transposase [Thermoanaerobacter
UniRef100_Q8RCM3

Thermoanaerobacter

tengcongensis]

tengcongensis

658
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81ZG4

Bacillus anthracis

659
Putative adenine deaminase yerA
UniRef100_O34909

Bacillus subtilis

YerA

[Bacillus subtilis]

660
YerB protein [Bacillus subtilis]
UniRef100_O34968

Bacillus subtilis

YerB

661
YecD [Bacillus subtilis]
UniRef100_Q7BVT7

Bacillus subtilis

YerC

662
PcrB protein homolog [Bacillus subtilis]
UniRef100_O34790

Bacillus subtilis

PcrB

663
ATP-dependent DNA helicase pcrA
UniRef100_O34580

Bacillus subtilis

PcrA

[Bacillus subtilis]

664
DNA ligase [Bacillus subtilis]
UniRef100_O31498

Bacillus subtilis

LigA

665
YerH protein [Bacillus subtilis]
UniRef100_O34629

Bacillus subtilis

YerH

666
BH0586 protein [Bacillus halodurans]
UniRef100_Q9KF99

Bacillus halodurans

667

668

669
BH0589 protein [Bacillus halodurans]
UniRef100_Q9KF96

Bacillus halodurans

670
Phosphotriesterase homology protein
UniRef100_P45548

Escherichia coli

[Escherichia coli]

671
Similar to unknown protein YhfS of
UniRef100_Q7N5F2

Photorhabdus

Csd

Escherichia coli [Photorhabdus

luminescens

luminescens]

672
Phosphopentomutase
UniRef100_Q67S06

Symbiobacterium

Drm

[Symbiobacterium thermophilum]

thermophilum

673
Putative alanine racemase
UniRef100_Q67S05

Symbiobacterium

[Symbiobacterium thermophilum]

thermophilum

674
Glucosamine-6-phosphate deaminase
UniRef100_Q8ESL6

Oceanobacillus

NagB

[Oceanobacillus iheyensis]

iheyensis

675
SapB protein [Bacillus subtilis]
UniRef100_Q45514

Bacillus subtilis

SapB

676

OpuE

677
Glutamyl-tRNA(Gln) amidotransferase
UniRef100_O06492

Bacillus subtilis

subunit C [Bacillus subtilis]

678
Glutamyl-tRNA(Gln) amidotransferase
UniRef100_O06491

Bacillus subtilis

GatA

subunit A [Bacillus subtilis]

679

GatB

680
Hypothetical protein [Bacillus
UniRef100_Q848Y2

Bacillus megaterium

megaterium]

681

682

YdhT

683

684
Putative HTH-type transcriptional
UniRef100_O31500

Bacillus subtilis

YerO

regulator yerO [Bacillus subtilis]

685
Swarming motility protein swrC [Bacillus
UniRef100_O31501

Bacillus subtilis

YerP

subtilis]

686

YjcK

687
Inosine-uridine preferring nucleoside
UniRef100_Q81DM6

Bacillus cereus

hydrolase [Bacillus cereus]

688
YerQ protein [Bacillus subtilis]
UniRef100_O31502

Bacillus subtilis

YerQ

689
Hypothetical RNA methyltransferase
UniRef100_O31503

Bacillus subtilis

YefA

yefA [Bacillus subtilis]

690
Type I restriction-modification system
UniRef100_Q817S1

Bacillus cereus

specificity subunit [Bacillus cereus]

691
Type I restriction-modification system
UniRef100_Q817S2

Bacillus cereus

methylation subunit [Bacillus cereus]

692
Type IC specificity subunit
UniRef100_Q9RNW0

Streptococcus thermophilus

[Streptococcus thermophilus]

693
Type I restriction-modification system
UniRef100_Q817S4

Bacillus cereus

restriction subunit [Bacillus cereus]

694

695
Beta-glucosides PTS, EIIBCA
UniRef100_Q88T54

Lactobacillus

BglP

[Lactobacillus plantarum]

plantarum

696
6-phospho-beta-glucosidase
UniRef100_Q88T55

Lactobacillus

BglH

[Lactobacillus plantarum]

plantarum

697

LicT

698
Response regulator aspartate
UniRef100_O34930

Bacillus subtilis

RapK

phosphatase K [Bacillus subtilis]

699

700
Methyltransferase [Bacillus cereus ZK]
UniRef100_Q639N2

Bacillus cereus ZK

701
Hypothetical UPF0082 protein
UniRef100_P62032

Bacillus cereus

YeeI

BCE0595 [Bacillus cereus]

702
Putative HTH-type transcriptional
UniRef100_O28646

Archaeoglobus fulgidus

regulator AF1627 [Archaeoglobus

fulgidus]

703

704
YfmT [Bacillus subtilis]
UniRef100_O06478

Bacillus subtilis

YfmT

705
YfmS [Bacillus subtilis]
UniRef100_O06477

Bacillus subtilis

YfmS

706

YflS

707
YfmR [Bacillus subtilis]
UniRef100_O06476

Bacillus subtilis

YfmR

708

709
YciA protein [Bacillus subtilis]
UniRef100_P94398

Bacillus subtilis

YciA

710
UPI00002BDF65 UniRef100 entry
UniRef100_UPI00002BDF65

YpdA

711
Ferrusion transporter protein
UniRef100_Q6U5S9

Klebsiella pneumoniae

[Klebsiella pneumoniae]

712

713
YciC protein [Bacillus
UniRef100_Q70KK8

Bacillus

YciC

amyloliquefaciens]

amyloliquefaciens

714

BioW

715
Adenosylmethionine-8-amino-7-
UniRef100_P53555

Bacillus subtilis

BioA

oxononanoate aminotransferase

[Bacillus subtilis]

716
8-amino-7-oxononanoate synthase
UniRef100_P53556

Bacillus subtilis

BioF

[Bacillus subtilis]

717
BioD protein [Bacillus
UniRef100_Q70JZ0

Bacillus

BioD

amyloliquefaciens]

amyloliquefaciens

718
BioB protein [Bacillus
UniRef100_Q70JZ1

Bacillus

BioB

amyloliquefaciens]

amyloliquefaciens

719
BioI protein [Bacillus amyloliquefaciens]
UniRef100_Q70JZ2

Bacillus

BioI

amyloliquefaciens

720
BH1501 protein [Bacillus halodurans]
UniRef100_Q9KCR8

Bacillus halodurans

721

YfmP

722
Multidrug efflux protein yfmO [Bacillus
UniRef100_O06473

Bacillus subtilis

YfmO

subtilis]

723
YfmM protein [Bacillus subtilis]
UniRef100_O34512

Bacillus subtilis

YfmM

724
YfmL protein [Bacillus subtilis]
UniRef100_O34750

Bacillus subtilis

YfmL

725
YfmJ protein [Bacillus subtilis]
UniRef100_O34812

Bacillus subtilis

YfmJ

726
Hypothetical protein yfmB [Bacillus
UniRef100_O34626

Bacillus subtilis

YfmB

subtilis]

727
General stress protein 17M [Bacillus
UniRef100_P80241

Bacillus subtilis

YflT

subtilis]

728

YflS

729

YflS

730
Putative permease [Clostridium tetani]
UniRef100_Q895A0

Clostridium tetani

731
Possible Zn-dependent hydrolase,
UniRef100_Q6HKH5

Bacillus

YqgX

beta-lactamase superfamily [Bacillus

thuringiensis

thuringiensis]

732
YflN protein [Bacillus subtilis]
UniRef100_O34409

Bacillus subtilis

YflN

733
Zink-carboxypeptidase [Clostridium
UniRef100_Q898E1

Clostridium tetani

tetani]

734
Nitric oxide synthase oxygenase
UniRef100_O34453

Bacillus subtilis

YflM

[Bacillus subtilis]

735
Membrane protein, putative [Bacillus
UniRef100_Q72YN4

Bacillus cereus

YvaZ

cereus]

736
Transcriptional regulator, ArsR family
UniRef100_Q632K6

Bacillus cereus ZK

[Bacillus cereus ZK]

737
Putative acylphosphatase [Bacillus
UniRef100_O35031

Bacillus subtilis

subtilis]

738
YflK protein [Bacillus subtilis]
UniRef100_O34542

Bacillus subtilis

YflK

739

740

741
YflG protein [Bacillus subtilis]
UniRef100_O34484

Bacillus subtilis

YflG

742

YflE

743
Hypothetical protein yflB [Bacillus
UniRef100_O34887

Bacillus subtilis

subtilis]

744

YflA

745
Probable PTS system, trehalose-
UniRef100_P39794

Bacillus subtilis

TreP

specific IIBC component [Bacillus

subtilis]

746
Alpha-glucosidase [Bacillus sp.
UniRef100_Q9L872

Bacillus sp.
TreA

DG0303]

DG0303

747
Trehalose operon transcriptional
UniRef100_P39796

Bacillus subtilis

TreR

repressor [Bacillus subtilis]

748
Acetyltransferases
UniRef100_Q8RBZ9

Thermoanaerobacter

YvfD

[Thermoanaerobacter tengcongensis]

tengcongensis

749

750

751
Predicted pyridoxal phosphate-
UniRef100_Q8RBY7

Thermoanaerobacter

SpsC

dependent enzyme apparently involved

tengcongensis

in regulation of cell wall biogenesis

[Thermoanaerobacter tengcongensis]

752

YkuQ

753
UPI000029FB28 UniRef100 entry
UniRef100_UPI000029FB28

YtcB

754
Hypothetical protein MA2181
UniRef100_Q8TNU7

Methanosarcina acetivorans

[Methanosarcina acetivorans]

755

756
Beta 1,4 glucosyltransferase [Bacillus
UniRef100_Q81GJ1

Bacillus cereus

YolJ

cereus]

757
Predicted pyridoxal phosphate-
UniRef100_Q8RC02

Thermoanaerobacter

SpsC

dependent enzyme apparently involved

tengcongensis

in regulation of cell wall biogenesis

[Thermoanaerobacter tengcongensis]

758
Predicted dehydrogenases and related
UniRef100_Q8RC00

Thermoanaerobacter

YrbE

proteins [Thermoanaerobacter

tengcongensis

tengcongensis]

759
UDP-glucose: GDP-mannose
UniRef100_Q8CXB6

Oceanobacillus

TuaD

dehydrogenase [Oceanobacillus

iheyensis

iheyensis]

760
Hypothetical protein [Bacillus cereus
UniRef100_Q635H5

Bacillus cereus

YqkD

ZK]

ZK

761
Hypothetical UPF0087 protein ydeP
UniRef100_P96673

Bacillus subtilis

YdeP

[Bacillus subtilis]

762
Putative NAD(P)H nitroreductase yfkO
UniRef100_O34475

Bacillus subtilis

YfkO

[Bacillus subtilis]

763

YfkN

764
General stress protein 18 [Bacillus
UniRef100_P80876

Bacillus subtilis

YfkM

subtilis]

765
YfkK protein [Bacillus subtilis]
UniRef100_O35019

Bacillus subtilis

766
Amino acid transporter [Bacillus
UniRef100_Q9K5Q5

Bacillus

YflA

halodurans]

halodurans

767
YfkJ protein [Bacillus subtilis]
UniRef100_O35016

Bacillus subtilis

YfkJ

768
Hypothetical protein yfkI precursor
UniRef100_O34418

Bacillus subtilis

YfkI

[Bacillus subtilis]

769
YfkH protein [Bacillus subtilis]
UniRef100_O34437

Bacillus subtilis

YfkH

770
YfkF protein [Bacillus subtilis]
UniRef100_O34929

Bacillus subtilis

YfkF

771
Hypothetical conserved protein
UniRef100_Q8ELS1

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

772
YfkE protein [Bacillus subtilis]
UniRef100_O34840

Bacillus subtilis

YfkE

773
YfkD protein [Bacillus subtilis]
UniRef100_O34579

Bacillus subtilis

YfkD

774
Thioredoxin-like oxidoreductases
UniRef100_Q81IC7

Bacillus cereus

YfkA

[Bacillus cereus]

775
YfjT protein [Bacillus subtilis]
UniRef100_O35041

Bacillus subtilis

776

YfjS

777
UPI000029390C UniRef100 entry
UniRef100_UPI000029390C

AraM

778

779
YfjQ protein [Bacillus subtilis]
UniRef100_O31543

Bacillus subtilis

YfjQ

780
YfjP protein [Bacillus subtilis]
UniRef100_O31544

Bacillus subtilis

YfjP

781

YfjO

782
YfjM protein [Bacillus subtilis]
UniRef100_O31547

Bacillus subtilis

YfjM

783

784
Hypothetical protein yfjL [Bacillus
UniRef100_P40773

Bacillus subtilis

YfjL

subtilis]

785
UPI00003CB259 UniRef100 entry
UniRef100_UPI00003CB259

YvkB

786
YdhE protein [Bacillus subtilis]
UniRef100_O05496

Bacillus subtilis

YdhE

787
Hypothetical protein yckD precursor
UniRef100_P42402

Bacillus subtilis

[Bacillus subtilis]

788
Hypothetical metabolite transport
UniRef100_O34691

Bacillus subtilis

YceI

protein yceI [Bacillus subtilis]

789

SacX

790
Levansucrase and sucrase synthesis
UniRef100_P15401

Bacillus subtilis

SacY

operon antiterminator [Bacillus subtilis]

791
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81NL1

Bacillus

YbcF

anthracis

792
Hypothetical protein ybcD [Bacillus
UniRef100_Q639F7

Bacillus cereus

YbcD

cereus ZK]

ZK

793
Potential NADH-quinone
UniRef100_P39755

Bacillus subtilis

NdhF

oxidoreductase subunit 5 [Bacillus

subtilis]

794
YbcI protein [Bacillus subtilis]
UniRef100_O34380

Bacillus subtilis

YbcI

795

YraA

796
TPP-dependent acetoin dehydrogenase
UniRef100_Q81PM6

Bacillus

AcoA

E1 alpha-subunit [Bacillus anthracis]

anthracis

797
TPP-dependent acetoin dehydrogenase
UniRef100_Q736U7

Bacillus cereus

AcoB

E1 beta-subunit [Bacillus cereus]

798
Dihydrolipoyllysine-residue
UniRef100_O31550

Bacillus subtilis

AcoC

acetyltransferase component of acetoin

cleaving system [Bacillus subtilis]

799
Dihydrolipoyl dehydrogenase [Bacillus
UniRef100_O34324

Bacillus subtilis

AcoL

subtilis]

800
Acetoin operon transcriptional activator,
UniRef100_Q736V6

Bacillus cereus

AcoR

putative [Bacillus cereus]

801
Hypothetical UPF0060 protein yfjF
UniRef100_O31553

Bacillus subtilis

YfjF

[Bacillus subtilis]

802
Maltose-6′-phosphate glucosidase
UniRef100_P54716

Bacillus subtilis

MalA

[Bacillus subtilis]

803
HTH-type transcriptional regulator glvR
UniRef100_P54717

Bacillus subtilis

YfiA

[Bacillus subtilis]

804
PTS system, arbutin-like IIBC
UniRef100_P54715

Bacillus subtilis

MalP

component [Bacillus subtilis]

805
UPI00003651CF UniRef100 entry
UniRef100_UPI00003651CF

806
UPI000034AA1D UniRef100 entry
UniRef100_UPI000034AA1D

807
Transcriptional regulator, MarR family
UniRef100_Q638I2

Bacillus cereus

YvaP

[Bacillus cereus ZK]

ZK

808

YfiD

809
Hypothetical protein yfiE [Bacillus
UniRef100_P54721

Bacillus subtilis

YfiE

subtilis]

810
Xylosidase/arabinosidase [Bacteroides
UniRef100_Q8A036

Bacteroides

XynB

thetaiotaomicron]

thetaiotaomicron

811
Xylan beta-1,4-xylosidase [Bacillus
UniRef100_Q9K6P5

Bacillus

XynB

halodurans]

halodurans

812
Trancriptional regulator of AraC family
UniRef100_Q97FW8

Clostridium

YbfI

[Clostridium acetobutylicum]

acetobutylicum

813

YtcQ

814
NAD(P)H dehydrogenase, quinone
UniRef100_Q638S8

Bacillus cereus ZK

family [Bacillus cereus ZK]

815
Mutator MutT protein [Bacillus
UniRef100_Q9K8B7

Bacillus

YjhB

halodurans]

halodurans

816
YfiT protein [Bacillus subtilis]
UniRef100_O31562

Bacillus subtilis

YfiT

817
YfiX [Bacillus subtilis]
UniRef100_O52961

Bacillus subtilis

YfiX

818
Hypothetical protein yfhB [Bacillus
UniRef100_O31570

Bacillus subtilis

YfhB

subtilis]

819
YfhC protein [Bacillus subtilis]
UniRef100_O31571

Bacillus subtilis

YfhC

820
Hypothetical protein yfhD [Bacillus
UniRef100_O31572

Bacillus subtilis

subtilis]

821

822
BH0923 homolog [Bacillus cereus]
UniRef100_Q81IA0

Bacillus cereus

823
Hypothetical UPF0105 protein yfhF
UniRef100_O31574

Bacillus subtilis

YfhF

[Bacillus subtilis]

824
Regulatory protein recX [Bacillus
UniRef100_O31575

Bacillus subtilis

YfhG

subtilis]

825
YfhH protein [Bacillus subtilis]
UniRef100_O31576

Bacillus subtilis

YfhH

826

827
YfhJ protein [Bacillus subtilis]
UniRef100_O31578

Bacillus subtilis

828
CsbB protein [Bacillus subtilis]
UniRef100_Q45539

Bacillus subtilis

CsbB

829
Hypothetical protein SE1997
UniRef100_Q8CR87

Staphylococcus epidermidis

[Staphylococcus epidermidis]

830
YfhO protein [Bacillus subtilis]
UniRef100_O31582

Bacillus subtilis

YfhO

831
YfhP protein [Bacillus subtilis]
UniRef100_O31583

Bacillus subtilis

YfhP

832
YfhQ protein [Bacillus subtilis]
UniRef100_O31584

Bacillus subtilis

YfhQ

833
YfhS protein [Bacillus subtilis]
UniRef100_O31585

Bacillus subtilis

834
Unidentfied dehydrogenase [Bacillus
UniRef100_P71079

Bacillus subtilis

FabL

subtilis]

835

836
Hypothetical protein ygaB [Bacillus
UniRef100_P71080

Bacillus subtilis

subtilis]

837
YgaC protein [Bacillus subtilis]
UniRef100_Q796Z1

Bacillus subtilis

YgaC

838
Unidentified transporter-ATP binding
UniRef100_P71082

Bacillus subtilis

YgaD

[Bacillus subtilis]

839
Oligopeptide ABC transporter [Bacillus
UniRef100_Q9K6T0

Bacillus

AppD

halodurans]

halodurans

840
Oligopeptide ABC transporter [Bacillus
UniRef100_Q9K6T1

Bacillus

AppF

halodurans]

halodurans

841
Dipeptide transporter protein DppA
UniRef100_P94310

Bacillus firmus

OppA

[Bacillus firmus]

842
Dipeptide ABC transporter [Bacillus
UniRef100_Q9K6T3

Bacillus

AppB

halodurans]

halodurans

843
Dipeptide transport system permease
UniRef100_P94312

Bacillus

AppC

protein dppC [Bacillus pseudofirmus]

pseudofirmus

844
Hypothetical 40.7 kd protein [Bacillus
UniRef100_P71083

Bacillus subtilis

YgaE

subtilis]

845
Glutamate-1-semialdehyde 2,1-
UniRef100_P71084

Bacillus subtilis

GsaB

aminomutase 2 [Bacillus subtilis]

846
YgaF protein [Bacillus subtilis]
UniRef100_Q796Y8

Bacillus subtilis

YgaF

847
Peroxide operon regulator [Bacillus
UniRef100_P71086

Bacillus subtilis

PerR

subtilis]

848

849
Hypothetical protein ygxA [Bacillus
UniRef100_Q04385

Bacillus subtilis

YgxA

subtilis]

850

RapD

851

852

853

854

855

856
Hypothetical protein [Bacillus
UniRef100_Q6HH72

Bacillus thuringiensis

thuringiensis]

857

YxiD

858
Hypothetical protein [uncultured
UniRef100_Q64DF2
uncultured archaeon

archaeon GZfos18F2]

GZfos18F2

859

860

YxiD

861

RapE

862
Putative membrane protein
UniRef100_Q82NM0

Streptomyces avermitilis

[Streptomyces avermitilis]

863
3-dehydroquinate dehydratase [Listeria
UniRef100_Q8Y9N4

Listeria

AroC

monocytogenes]

monocytogenes

864

ThiC

865
Putative aliphatic sulfonates transport
UniRef100_P97027

Bacillus subtilis

SsuB

ATP-binding protein ssuB [Bacillus

subtilis]

866
Putative aliphatic sulfonates binding
UniRef100_P40400

Bacillus subtilis

SsuA

protein precursor [Bacillus subtilis]

867
Putative aliphatic sulfonates transport
UniRef100_P40401

Bacillus subtilis

SsuC

permease protein ssuC [Bacillus

subtilis]

868

SsuD

869

SsuD

870
Hypothetical lipoprotein ygaO precursor
UniRef100_P97029

Bacillus subtilis

YgaO

[Bacillus subtilis]

871
DNA-binding protein [Bacillus anthracis]
UniRef100_Q81V18

Bacillus anthracis

872

873
ABC-type multidrug transport system,
UniRef100_Q8RBH0

Thermoanaerobacter

YhaQ

ATPase component

tengcongensis

[Thermoanaerobacter tengcongensis]

874

875

876

877

878
30S ribosomal protein S14-2 [Bacillus
UniRef100_O31587

Bacillus subtilis

subtilis]

879
Hypothetical protein yhzB [Bacillus
UniRef100_O31588

Bacillus subtilis

YhzB

subtilis]

880
Hypothetical 48.5 kd protein [Bacillus
UniRef100_P97030

Bacillus subtilis

YhbA

subtilis]

881
Hypothetical 35.8 kd protein [Bacillus
UniRef100_P97031

Bacillus subtilis

YhbB

subtilis]

882
CspR [Bacillus subtilis]
UniRef100_Q45512

Bacillus subtilis

CspR

883
Hypothetical 27 kd protein [Bacillus
UniRef100_P97032

Bacillus subtilis

YhbD

subtilis]

884
Hypothetical Cytosolic Protein [Bacillus
UniRef100_Q813H1

Bacillus cereus

YhbE

cereus]

885
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81PD2

Bacillus

YhbF

anthracis

886

887
PrkA protein [Bacillus subtilis]
UniRef100_P39134

Bacillus subtilis

PrkA

888
Stress response UPF0229 protein yhbH
UniRef100_P45742

Bacillus subtilis

YhbH

[Bacillus subtilis]

889
YhbJ protein [Bacillus subtilis]
UniRef100_O31593

Bacillus subtilis

YhbJ

890
Hypothetical transport protein yhcA
UniRef100_P54585

Bacillus subtilis

YhcA

[Bacillus subtilis]

891
Hypothetical protein yhcB [Bacillus
UniRef100_P54586

Bacillus subtilis

YhcB

subtilis]

892

YhcC

893

894
Hypothetical ABC transporter ATP-
UniRef100_P54591

Bacillus subtilis

binding protein yhcG [Bacillus subtilis]

895
Hypothetical ABC transporter ATP-
UniRef100_P54592

Bacillus subtilis

YhcH

binding protein yhcH [Bacillus subtilis]

896
Hypothetical protein yhcI [Bacillus
UniRef100_P54593

Bacillus subtilis

YhcI

subtilis]

897
Cold shock protein cspB [Bacillus
UniRef100_P32081

Bacillus subtilis

subtilis]

898
ABC transporter, ATP-binding protein
UniRef100_Q6HP89

Bacillus

YusC

[Bacillus thuringiensis]

thuringiensis

899
MW0417 protein [Staphylococcus
UniRef100_Q8NY20

Staphylococcus

YusB

aureus]

aureus

900
ABC transporter substrate-binding
UniRef100_Q81IN6

Bacillus cereus

YhcJ

protein [Bacillus cereus]

901
Hypothetical symporter yhcL [Bacillus
UniRef100_P54596

Bacillus subtilis

YhcL

subtilis]

902
Hypothetical protein yhcM [Bacillus
UniRef100_P54597

Bacillus subtilis

YhcM

subtilis]

903
Acylamino-acid-releasing enzyme
UniRef100_Q8CXN6

Oceanobacillus

YuxL

[Oceanobacillus iheyensis]

iheyensis

904
Lipoprotein yhcN precursor [Bacillus
UniRef100_P54598

Bacillus subtilis

YhcN

subtilis]

905
Hypothetical protein yhcP [Bacillus
UniRef100_P54600

Bacillus subtilis

YhcP

subtilis]

906
Hypothetical protein yhcQ [Bacillus
UniRef100_P54601

Bacillus subtilis

YhcQ

subtilis]

907
Two-component sensor histidine kinase
UniRef100_O31661

Bacillus subtilis

KinE

[Bacillus subtilis]

908
Hypothetical protein yhcR precursor
UniRef100_P54602

Bacillus subtilis

YhcR

[Bacillus subtilis]

909
Hypothetical protein yhcS [Bacillus
UniRef100_P54603

Bacillus subtilis

YhcS

subtilis]

910
Hypothetical pseudouridine synthase
UniRef100_P54604

Bacillus subtilis

YhcT

yhcT [Bacillus subtilis]

911
Hypothetical protein yhcU [Bacillus
UniRef100_P54605

Bacillus subtilis

YhcU

subtilis]

912
Hypothetical protein yhcV [Bacillus
UniRef100_P54606

Bacillus subtilis

YhcV

subtilis]

913
Hypothetical protein yhcW [Bacillus
UniRef100_P54607

Bacillus subtilis

YhcW

subtilis]

914
Hypothetical UPF0012 protein yhcX
UniRef100_P54608

Bacillus subtilis

YhcX

[Bacillus subtilis]

915
ABC transporter [Bacillus halodurans]
UniRef100_Q9KBA3

Bacillus

YdiF

halodurans

916
Hypothetical transport protein yrhG
UniRef100_O05399

Bacillus subtilis

YrhG

[Bacillus subtilis]

917
Lin0826 protein [Listeria innocua]
UniRef100_Q92DI7

Listeria innocua

YwkB

918
Alcohol dehydrogenase [Bacillus
UniRef100_Q81CT4

Bacillus cereus

YogA

cereus]

919
Glycerol uptake operon antiterminator
UniRef100_P30300

Bacillus subtilis

GlpP

regulatory protein [Bacillus subtilis]

920
Glycerol uptake facilitator protein
UniRef100_P18156

Bacillus subtilis

GlpF

[Bacillus subtilis]

921
Glycerol kinase [Bacillus subtilis]
UniRef100_P18157

Bacillus subtilis

GlpK

922
Aerobic glycerol-3-phosphate
UniRef100_P18158

Bacillus subtilis

GlpD

dehydrogenase [Bacillus subtilis]

923
Alpha-phosphoglucomutase [Bacillus
UniRef100_Q68VA2

Bacillus subtilis

YhxB

subtilis subsp. subtilis]

subsp. subtilis

924
Hypothetical conserved protein
UniRef100_Q8ELS7

Oceanobacillus

YcgB

[Oceanobacillus iheyensis]

iheyensis

925
Transcriptional regulator
UniRef100_Q8ELS8

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

926

YhcY

927
Hypothetical protein yhcZ [Bacillus
UniRef100_O07528

Bacillus subtilis

YhcZ

subtilis]

928
Hypothetical protein yhdA [Bacillus
UniRef100_O07529

Bacillus subtilis

YhdA

subtilis]

929

930

931
Hypothetical UPF0074 protein yhdE
UniRef100_O07573

Bacillus subtilis

YhdE

[Bacillus subtilis]

932
Flavohemoprotein [Bacillus halodurans]
UniRef100_Q9RC40

Bacillus

Hmp

halodurans

933
Stage V sporulation protein R [Bacillus
UniRef100_P37875

Bacillus subtilis

SpoVR

subtilis]

934
Probable endopeptidase IytE precursor
UniRef100_P54421

Bacillus subtilis

LytE

[Bacillus subtilis]

935

CitR

936

CitA

937
Glucose dehydrogenase-B [Bacillus
UniRef100_Q9Z9R3

Bacillus halodurans

halodurans]

938
Hypothetical oxidoreductase yhdF
UniRef100_O07575

Bacillus subtilis

YhdF

[Bacillus subtilis]

939
Hypothetical protein yhdH [Bacillus
UniRef100_O07577

Bacillus subtilis

YhdH

subtilis]

940
2,4-diaminobutyrate decarboxylase
UniRef100_Q9KFB9

Bacillus halodurans

[Bacillus halodurans]

941
YdeE protein [Bacillus subtilis]
UniRef100_P96662

Bacillus subtilis

YdeE

942
YdeL protein [Bacillus subtilis]
UniRef100_P96669

Bacillus subtilis

YdeL

943
BH1582 protein [Bacillus halodurans]
UniRef100_Q9KCI9

Bacillus

YhdJ

halodurans

944
YhdK [Bacillus subtilis subsp. spizizenii]
UniRef100_Q7X2K9

Bacillus subtilis subsp.

spizizenii

945
YhdL [Bacillus subtilis subsp. spizizenii]
UniRef100_Q7X2L0

Bacillus subtilis

YhdL

subsp. spizizenii

946
Hypothetical protein yhdM (RNA
UniRef100_O07582

Bacillus subtilis

SigM

polymerase ECF (Extracytoplasmic

function)-type sigma factor) [Bacillus

subtilis]

947

YrkC

948
Hypothetical protein yhdO [Bacillus
UniRef100_O07584

Bacillus subtilis

YhdO

subtilis]

949

950
Acyl-CoA thioesterase 1 [Clostridium
UniRef100_Q97DR5

Clostridium acetobutylicum

acetobutylicum]

951
UPI00003CB259 UniRef100 entry
UniRef100_UPI00003CB259

YvkB

952

YhdP

953
HTH-type transcriptional regulator cueR
UniRef100_O07586

Bacillus subtilis

YhdQ

[Bacillus subtilis]

954

YhdT

955

956
BH3511 protein [Bacillus halodurans]
UniRef100_Q9K762

Bacillus halodurans

957
Sporulation specific N-acetylmuramoyl-
UniRef100_Q8CX69

Oceanobacillus

CwlC

L-alanine amidase [Oceanobacillus

iheyensis

iheyensis]

958
Protein crcB homolog 1 [Bacillus
UniRef100_O07590

Bacillus subtilis

YhdU

subtilis]

959
Protein crcB homolog 2 [Bacillus
UniRef100_O07591

Bacillus subtilis

YhdV

subtilis]

960

YhdW

961

962
Hypothetical UPF0003 protein yhdY
UniRef100_O07594

Bacillus subtilis

YhdY

[Bacillus subtilis]

963
NAD-dependent deacetylase [Bacillus
UniRef100_O07595

Bacillus subtilis

YhdZ

subtilis]

964

965
Hypothetical protein yheN [Bacillus
UniRef100_O07596

Bacillus subtilis

YheN

subtilis]

966

Dat

967
Na(+)/H(+) antiporter [Bacillus subtilis]
UniRef100_O07553

Bacillus subtilis

NhaC

968
Hypothetical protein yoxA [Bacillus
UniRef100_P39840

Bacillus subtilis

YoxA

subtilis]

969
Hypothetical protein ydhH [Bacillus
UniRef100_O05500

Bacillus subtilis

YdhH

subtilis]

970
Hypothetical protein [Bacillus cereus
UniRef100_Q63EB4

Bacillus cereus ZK

ZK]

971
Hypothetical protein [Bacillus cereus]
UniRef100_Q81GF4

Bacillus cereus

972
Stress response protein nhaX [Bacillus
UniRef100_O07552

Bacillus subtilis

NhaX

subtilis]

973
Hypothetical protein yheI [Bacillus
UniRef100_O07550

Bacillus subtilis

YheI

subtilis]

974
Hypothetical protein yheH [Bacillus
UniRef100_O07549

Bacillus subtilis

YheH

subtilis]

975
Hypothetical protein yheG [Bacillus
UniRef100_O07548

Bacillus subtilis

YheG

subtilis]

976
Small, acid-soluble spore protein B
UniRef100_P04832

Bacillus subtilis

[Bacillus subtilis]

977
BH1139 protein [Bacillus halodurans]
UniRef100_Q9KDS2

Bacillus halodurans

978
Sugar ABC transporter ATP-binding
UniRef100_Q8CUH3

Oceanobacillus

MsmX

protein [Oceanobacillus iheyensis]

iheyensis

979
Fis-type helix-turn-helix domain protein
UniRef100_Q73A84

Bacillus cereus

YxkF

[Bacillus cereus]

980
Hypothetical protein yheE [Bacillus
UniRef100_O07546

Bacillus subtilis

subtilis]

981
Hypothetical protein yheD [Bacillus
UniRef100_O07545

Bacillus subtilis

YheD

subtilis]

982
Hypothetical protein yheC [Bacillus
UniRef100_O07544

Bacillus subtilis

YheC

subtilis]

983
Hypothetical protein yheB [Bacillus
UniRef100_O07543

Bacillus subtilis

YheB

subtilis]

984
Hypothetical protein yheA [Bacillus
UniRef100_O07542

Bacillus subtilis

YheA

subtilis]

985
Stress response protein yhaX [Bacillus
UniRef100_O07539

Bacillus subtilis

YhaX

subtilis]

986

HemZ

987
YhaR protein [Bacillus subtilis]
UniRef100_O07533

Bacillus subtilis

YhaR

988
Response regulator aspartate
UniRef100_P96649

Bacillus subtilis

RapI

phosphatase I [Bacillus subtilis]

989

990
Hypothetical protein yhaQ [Bacillus
UniRef100_O07524

Bacillus subtilis

YhaQ

subtilis]

991
Hypothetical protein yhaP [Bacillus
UniRef100_O07523

Bacillus subtilis

YhaP

subtilis]

992

YhaO

993
Hypothetical protein yhaN [Bacillus
UniRef100_O08455

Bacillus subtilis

YhaN

subtilis]

994

YhaM

995
Hypothetical protein yhaL [Bacillus
UniRef100_O07520

Bacillus subtilis

subtilis]

996
Foldase protein prsA precursor
UniRef100_P24327

Bacillus subtilis

PrsA

[Bacillus subtilis]

997

998
Hypothetical protein yhaK [Bacillus
UniRef100_O07519

Bacillus subtilis

subtilis]

999
Hypothetical protein yhaI [Bacillus
UniRef100_O07517

Bacillus subtilis

YhaI

subtilis]

1000
Protease production regulatory protein
UniRef100_P11065

Bacillus subtilis

Hpr

hpr [Bacillus subtilis]

1001
Hypothetical protein yhaH [Bacillus
UniRef100_O07516

Bacillus subtilis

YhaH

subtilis]

1002
Probable tryptophan transport protein
UniRef100_O07515

Bacillus subtilis

YhaG

[Bacillus subtilis]

1003
Phosphoserine aminotransferase
UniRef100_P80862

Bacillus subtilis

SerC

[Bacillus subtilis]

1004
Hit protein [Bacillus subtilis]
UniRef100_O07513

Bacillus subtilis

Hit

1005

1006
ABC-type transporter ATP-binding
UniRef100_P55339

Bacillus subtilis

EcsA

protein ecsA [Bacillus subtilis]

1007
Protein ecsB [Bacillus subtilis]
UniRef100_P55340

Bacillus subtilis

EcsB

1008
Protein ecsC [Bacillus subtilis]
UniRef100_P55341

Bacillus subtilis

EcsC

1009
YhaA protein [Bacillus subtilis]
UniRef100_O07598

Bacillus subtilis

YhaA

1010
Hypothetical protein yhfA [Bacillus
UniRef100_O07599

Bacillus subtilis

YhfA

subtilis]

1011
Hypothetical protein yhgC [Bacillus
UniRef100_P38049

Bacillus subtilis

YhgC

subtilis]

1012
Penicillin-binding protein 1F [Bacillus
UniRef100_P38050

Bacillus subtilis

PbpF

subtilis]

1013
Uroporphyrinogen decarboxylase
UniRef100_P32395

Bacillus subtilis

HemE

[Bacillus subtilis]

1014
Ferrochelatase [Bacillus subtilis]
UniRef100_P32396

Bacillus subtilis

HemH

1015
Protoporphyrinogen oxidase [Bacillus
UniRef100_P32397

Bacillus subtilis

HemY

subtilis]

1016

YhgD

1017
Hypothetical protein yhgE [Bacillus
UniRef100_P32399

Bacillus subtilis

YhgE

subtilis]

1018
3-oxoacyl-[acyl-carrier-protein]
UniRef100_O07600
acyl-carrier-
FabHB

synthase III protein 2 [Bacillus subtilis]

protein

1019
Hypothetical protein yhfE [Bacillus
UniRef100_O07603

Bacillus subtilis

YhfE

subtilis]

1020

1021
Hypothetical protein yhfG [Bacillus
UniRef100_O07605

Bacillus subtilis

GltT

subtilis]

1022
Hypothetical protein yhfI [Bacillus
UniRef100_O07607

Bacillus subtilis

YhfI

subtilis]

1023
Hypothetical protein yhfJ [Bacillus
UniRef100_O07608

Bacillus subtilis

YhfJ

subtilis]

1024
Hypothetical protein yhfL [Bacillus
UniRef100_O07610

Bacillus subtilis

YhfL

subtilis]

1025
Hypothetical protein yhfM precursor
UniRef100_O07611

Bacillus subtilis

YhfM

[Bacillus subtilis]

1026
BH2909 protein [Bacillus halodurans]
UniRef100_Q9K8U3

Bacillus halodurans

1027
Branched-chain amino acid transporter
UniRef100_Q9K8U2

Bacillus

AzlC

[Bacillus halodurans]

halodurans

1028
BH2911 protein [Bacillus halodurans]
UniRef100_Q9K8U1

Bacillus halodurans

1029
Putative metalloprotease yhfN [Bacillus
UniRef100_P40769

Bacillus subtilis

YhfN

subtilis]

1030

AprE

1031
Transporter, drug/metabolite exporter
UniRef100_Q63D40

Bacillus cereus

YdeD

family [Bacillus cereus ZK]

ZK

1032
Hypothetical protein yhfQ [Bacillus
UniRef100_O07616

Bacillus subtilis

YhfQ

subtilis]

1033
YfmD protein [Bacillus subtilis]
UniRef100_O34933

Bacillus subtilis

YfmD

1034
YfmE protein [Bacillus subtilis]
UniRef100_O34832

Bacillus subtilis

YfmE

1035
Hypothetical protein yhfR [Bacillus
UniRef100_O07617

Bacillus subtilis

YhfR

subtilis]

1036
Heme-based aerotactic transducer
UniRef100_O07621

Bacillus subtilis

HemAT

hemAT [Bacillus subtilis]

1037
Rieske 2Fe—2S iron-sulfur protein,
UniRef100_Q73E94

Bacillus cereus

YhfW

putative [Bacillus cereus]

1038

YhxC

1039
Hypothetical protein yhzC [Bacillus
UniRef100_O31594

Bacillus subtilis

subtilis]

1040

ComK

1041

YhjD

1042

YhjE

1043
Signal peptidase I V [Bacillus subtilis]
UniRef100_O07560

Bacillus subtilis

SipV

1044
Minor extracellular protease epr
UniRef100_P16396

Bacillus subtilis

Epr

precursor [Bacillus subtilis]

1045
Putative permease [Klebsiella
UniRef100_Q765R6

Klebsiella

YybO

pneumoniae]

pneumoniae

1046
Hypothetical protein [Enterococcus
UniRef100_Q82ZQ4

Enterococcus

PucR

faecalis]

faecalis

1047
Putative allantoinase [Staphylococcus
UniRef100_Q9EV52

Staphylococcus

PucH

xylosus]

xylosus

1048
Peptidase, M20/M25/M40 family
UniRef100_Q82ZQ2

Enterococcus

YurH

[Enterococcus faecalis]

faecalis

1049
Hypothetical protein STY0574
UniRef100_Q8XFX7

Salmonella typhi

[Salmonella typhi]

1050
Ureidoglycolate dehydrogenase
UniRef100_Q838P9

Enterococcus

YjmC

[Enterococcus faecalis]

faecalis

1051
Ureidoglycolate dehydrogenase
UniRef100_Q838P9

Enterococcus

YjmC

[Enterococcus faecalis]

faecalis

1052
Hypothetical protein [Enterococcus
UniRef100_Q838Q3

Enterococcus

SucD

faecalis]

faecalis

1053
Hypothetical protein [Enterococcus
UniRef100_Q838Q3

Enterococcus faecalis

faecalis]

1054

1055
Carbamate kinase [Clostridium tetani]
UniRef100_Q890W1

Clostridium tetani

1056
Major facilitator family transporter
UniRef100_Q838Q1

Enterococcus

YcbE

[Enterococcus faecalis]

faecalis

1057
Hypothetical protein yjfA precursor
UniRef100_O34554

Bacillus subtilis

[Bacillus subtilis]

1058
Response regulator aspartate
UniRef100_O32294

Bacillus subtilis

RapG

phosphatase G [Bacillus subtilis]

1059

1060

1061

1062
Lacl-family transcription regulator
UniRef100_O34829

Bacillus subtilis

MsmR

[Bacillus subtilis]

1063
Multiple sugar-binding protein [Bacillus
UniRef100_O34335

Bacillus subtilis

MsmE

subtilis]

1064
Sugar transporter [Bacillus subtilis]
UniRef100_O34706

Bacillus subtilis

AmyD

1065
Sugar transporter [Bacillus subtilis]
UniRef100_O34518

Bacillus subtilis

AmyC

1066
Alpha-galactosidase [Bacillus subtilis]
UniRef100_O34645

Bacillus subtilis

MelA

1067
Hypothetical protein yhjN [Bacillus
UniRef100_O07568

Bacillus subtilis

YhjN

subtilis]

1068
Spore coat-associated protein JA
UniRef100_Q63FK5

Bacillus cereus ZK

[Bacillus cereus ZK]

1069
CotJB protein [Bacillus subtilis]
UniRef100_Q45537

Bacillus subtilis

1070
CotJC protein [Bacillus subtilis]
UniRef100_Q45538

Bacillus subtilis

CotJC

1071
Long-chain fatty-acid-CoA ligase
UniRef100_Q9KDT0

Bacillus

YngI

[Bacillus halodurans]

halodurans

1072
Hypothetical protein yhjO [Bacillus
UniRef100_O07569

Bacillus subtilis

YhjO

subtilis]

1073
Hypothetical protein [Bacillus cereus
UniRef100_Q63FZ3

Bacillus cereus

LytB

ZK]

ZK

1074
Sensor histidine kinase [Bacillus
UniRef100_Q6HNG3

Bacillus

PhoR

thuringiensis]

thuringiensis

1075
Two-component response regulator
UniRef100_Q81I36

Bacillus cereus

YclJ

[Bacillus cereus]

1076

YhjR

1077
Putative molybdate binding protein,
UniRef100_O32208

Bacillus subtilis

YvgL

YvgL [Bacillus subtilis]

1078
Putative molybdate transport protein,
UniRef100_O32209

Bacillus subtilis

YvgM

YvgM [Bacillus subtilis]

1079
ATP-dependent nuclease subunit B
UniRef100_P23477

Bacillus subtilis

AddB

[Bacillus subtilis]

1080
ATP-dependent nuclease subunit A
UniRef100_P23478

Bacillus subtilis

AddA

[Bacillus subtilis]

1081
Exonuclease sbcD homolog [Bacillus
UniRef100_P23479

Bacillus subtilis

SbcD

subtilis]

1082
Nuclease sbcCD subunit C [Bacillus
UniRef100_O06714

Bacillus subtilis

YirY

subtilis]

1083
Probable spore germination protein
UniRef100_O06716

Bacillus subtilis

gerPF [Bacillus subtilis]

1084
Probable spore germination protein
UniRef100_O06717

Bacillus subtilis

GerPE

gerPE [Bacillus subtilis]

1085
Probable spore germination protein
UniRef100_O06718

Bacillus subtilis

gerPD [Bacillus subtilis]

1086
Probable spore germination protein
UniRef100_O06719

Bacillus subtilis

GerPC

gerPC [Bacillus subtilis]

1087
Probable spore germination protein
UniRef100_O06720

Bacillus subtilis

gerPB [Bacillus subtilis]

1088
Probable spore germination protein
UniRef100_O06721

Bacillus subtilis

gerPA [Bacillus subtilis]

1089
Hypothetical protein yitR [Bacillus
UniRef100_O06753

Bacillus subtilis

subtilis]

1090

1091
Spore coat protein H [Bacillus cereus]
UniRef100_Q81EE9

Bacillus cereus

CotH

1092

CotG

1093
YisK [Bacillus subtilis]
UniRef100_O06724

Bacillus subtilis

YisK

1094
Hypothetical protein yloQ [Bacillus
UniRef100_Q63CF2

Bacillus cereus

YloQ

cereus ZK]

ZK

1095
YisL [Bacillus subtilis]
UniRef100_O06725

Bacillus subtilis

YisL

1096
Hypothetical protein yisN [Bacillus
UniRef100_O06727

Bacillus subtilis

YisN

subtilis]

1097
Asparagine synthetase [glutamine-
UniRef100_O05272
glutamine-
AsnO

hydrolyzing] 3 [Bacillus subtilis]

hydrolyzing

1098

NrgA

1099
Nitrogen regulatory PII protein
UniRef100_Q8ERT8

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

1100
YisQ [Bacillus subtilis]
UniRef100_O07940

Bacillus subtilis

YisQ

1101
Putative HTH-type transcriptional
UniRef100_P40331

Bacillus subtilis

YisR

regulator yisR [Bacillus subtilis]

1102
Acetyltransferase, GNAT family
UniRef100_Q63C80

Bacillus cereus

YokL

[Bacillus cereus ZK]

ZK

1103
HTH-type transcriptional regulator degA
UniRef100_P37947

Bacillus subtilis

DegA

[Bacillus subtilis]

1104
Hypothetical oxidoreductase yisS
UniRef100_P40332

Bacillus subtilis

YisS

[Bacillus subtilis]

1105
YisV protein [Bacillus subtilis]
UniRef100_Q796Q6

Bacillus subtilis

YisV

1106
Diaminobutyrate--pyruvate
UniRef100_Q9K9M1

Bacillus

GabT

transaminase [Bacillus halodurans]

halodurans

1107
L-2,4-diaminobutyrate decarboxylase
UniRef100_Q8YZR2

Anabaena sp.

[Anabaena sp.]

1108
AII0394 protein [Anabaena sp.]
UniRef100_Q8YZR3

Anabaena sp.

1109
BH2621 protein [Bacillus halodurans]
UniRef100_Q9K9M4

Bacillus halodurans

1110
BH2620 protein [Bacillus halodurans]
UniRef100_Q9K9M5

Bacillus halodurans

1111
BH2618 protein [Bacillus halodurans]
UniRef100_Q9K9M7

Bacillus halodurans

1112
YitI protein [Bacillus subtilis]
UniRef100_O06744

Bacillus subtilis

YitI

1113
Glr2355 protein [Gloeobacter violaceus]
UniRef100_Q7N129

Gloeobacter

YcdF

violaceus

1114
BH0411 protein [Bacillus halodurans]
UniRef100_Q9KFR6

Bacillus

YobV

halodurans

1115
5-methyltetrahydrofolate S-
UniRef100_Q9KCE1

Bacillus

YitJ

homocysteine methyltransferase

halodurans

[Bacillus halodurans]

1116
YitJ [Bacillus subtilis]
UniRef100_O06745

Bacillus subtilis

YitJ

1117
Hypothetical UPF0234 protein yitk
UniRef100_O06746

Bacillus subtilis

YitK

[Bacillus subtilis]

1118
YitL protein [Bacillus subtilis]
UniRef100_O06747

Bacillus subtilis

YitL

1119

1120

1121
Hypothetical UPF0230 protein yitS
UniRef100_P70945

Bacillus subtilis

YitS

[Bacillus subtilis]

1122
Hypothetical protein yitT [Bacillus
UniRef100_P39803

Bacillus subtilis

YitT

subtilis]

1123
Intracellular proteinase inhibitor
UniRef100_P39804

Bacillus subtilis

Ipi

[Bacillus subtilis]

1124
GMP reductase [Bacillus subtilis]
UniRef100_O05269

Bacillus subtilis

GuaC

1125

1126

1127
Putative orf protein [Bacillus subtilis]
UniRef100_P70947

Bacillus subtilis

YitU

1128
Putative orf protein [Bacillus subtilis]
UniRef100_P70948

Bacillus subtilis

YitV

1129

YitW

1130
N-acetyl-gamma-glutamyl-phosphate
UniRef100_P23715

Bacillus subtilis

ArgC

reductase [Bacillus subtilis]

1131
Arginine biosynthesis bifunctional
UniRef100_Q9ZJ14
Includes:
ArgJ

protein argJ [Includes: Glutamate N-

Glutamate N-

acetyltransferase (EC 2.3.1.35)

acetyltransferase

(Ornithine acetyltransferase) (Ornithine

(EC 2.3.1.35)

transacetylase) (OATase); Amino-acid

(Ornithine

acetyltransferase (EC 2.3.1.1) (N-

acetyltransferase)

acetylglutamate synthase) (AGS)]

(Ornithine

[Contai

transacetylase)

(OATase);

Amino-acid

acetyltransferase

(EC 2.3.1.1) (N-

acetylglutamate

synthase) (AGS)

1132
Acetylglutamate kinase [Bacillus
UniRef100_P36840

Bacillus subtilis

ArgB

subtilis]

1133
Acetylornithine aminotransferase
UniRef100_P36839

Bacillus subtilis

ArgD

[Bacillus subtilis]

1134
Carbamoyl-phosphate synthase,
UniRef100_P36838

Bacillus subtilis

CarA

arginine-specific, small chain [Bacillus

subtilis]

1135
Carbamoyl-phosphate synthase,
UniRef100_P18185

Bacillus subtilis

CarB

arginine-specific, large chain [Bacillus

subtilis]

1136
Ornithine carbamoyltransferase
UniRef100_P18186

Bacillus subtilis

ArgF

[Bacillus subtilis]

1137
Undecaprenyl-diphosphatase 1
UniRef100_Q81HV4

Bacillus cereus

YubB

[Bacillus cereus]

1138
YjzC protein [Bacillus subtilis]
UniRef100_O34585

Bacillus subtilis

1139

1140
Hypothetical protein yjaU [Bacillus
UniRef100_O35001

Bacillus subtilis

YjaU

subtilis]

1141
ArgF and med genes, partial and
UniRef100_O32435

Bacillus subtilis

YjaV

complete cds [Bacillus subtilis]

1142
Transcriptional activator protein med
UniRef100_O32436

Bacillus subtilis

Med

precursor [Bacillus subtilis]

1143
ComG operon repressor [Bacillus
UniRef100_O32437

Bacillus subtilis

subtilis]

1144
Hypothetical protein yjzB [Bacillus
UniRef100_O34891

Bacillus subtilis

subtilis]

1145
3-oxoacyl-[acyl-carrier-protein]
UniRef100_O34746
acyl-carrier-
FabHA

synthase III protein 1 [Bacillus subtilis]

protein

1146
Beta-ketoacyl-acyl carrier protein
UniRef100_O34340

Bacillus subtilis

FabF

synthase II [Bacillus subtilis]

1147
YjaZ protein [Bacillus subtilis]
UniRef100_O31596

Bacillus subtilis

YjaZ

1148
Oligopeptide transport ATP-binding
UniRef100_P42064

Bacillus subtilis

AppD

protein appD [Bacillus subtilis]

1149
Oligopeptide transport ATP-binding
UniRef100_P42065

Bacillus subtilis

AppF

protein appF [Bacillus subtilis]

1150
Oligopeptide-binding protein appA
UniRef100_P42061

Bacillus subtilis

AppA

precursor [Bacillus subtilis]

1151
Oligopeptide transport system
UniRef100_P42062

Bacillus subtilis

AppB

permease protein appB [Bacillus

subtilis]

1152
Oligopeptide transport system
UniRef100_P42063

Bacillus subtilis

AppC

permease protein appC [Bacillus

subtilis]

1153
Permease, putative [Bacillus cereus]
UniRef100_Q734Y3

Bacillus cereus

YvqJ

1154

YjbA

1155
Tryptophanyl-tRNA synthetase [Bacillus
UniRef100_P21656

Bacillus subtilis

TrpS

subtilis]

1156
Oligopeptide-binding protein oppA
UniRef100_P24141

Bacillus subtilis

OppA

precursor [Bacillus subtilis]

1157
Oligopeptide transport system
UniRef100_P24138

Bacillus subtilis

OppB

permease protein oppB [Bacillus

subtilis]

1158
Oligopeptide transport system
UniRef100_P24139

Bacillus subtilis

OppC

permease protein oppC [Bacillus

subtilis]

1159
Oligopeptide transport ATP-binding
UniRef100_P24136

Bacillus subtilis

OppD

protein oppD [Bacillus subtilis]

1160
Oligopeptide transport ATP-binding
UniRef100_P24137

Bacillus subtilis

OppF

protein oppF [Bacillus subtilis]

1161
YjbC protein [Bacillus subtilis]
UniRef100_O31601

Bacillus subtilis

YjbC

1162
Regulatory protein spx [Bacillus subtilis]
UniRef100_O31602

Bacillus subtilis

YjbD

1163
YjbE protein [Bacillus subtilis]
UniRef100_O31603

Bacillus subtilis

YjbE

1164
Adapter protein mecA 1 [Bacillus
UniRef100_P37958

Bacillus subtilis

MecA

subtilis]

1165
Hypothetical conserved protein
UniRef100_Q8ELH8

Oceanobacillus

YflP

[Oceanobacillus iheyensis]

iheyensis

1166
Hypothetical protein OB3248
UniRef100_Q8ELH9

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

1167
Hypothetical conserved protein
UniRef100_Q8ELI0

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

1168
Response regulator of citrate/malate
UniRef100_Q7ML23

Vibrio vulnificus

CitT

metabolism [Vibrio vulnificus]

1169
Sensor protein citS [Bacillus
UniRef100_Q9RC53

Bacillus

YufL

halodurans]

halodurans

1170
YjbF protein [Bacillus subtilis]
UniRef100_O31604

Bacillus subtilis

YjbF

1171

YjbG

1172

1173
YjbH protein [Bacillus subtilis]
UniRef100_O31606

Bacillus subtilis

YjbH

1174
YjbI protein [Bacillus subtilis]
UniRef100_O31607

Bacillus subtilis

YjbI

1175
YjbJ protein [Bacillus subtilis]
UniRef100_O31608

Bacillus subtilis

YjbJ

1176
YjbK protein [Bacillus subtilis]
UniRef100_O31609

Bacillus subtilis

YjbK

1177
YjbL protein [Bacillus subtilis]
UniRef100_O31610

Bacillus subtilis

YjbL

1178
YjbM protein [Bacillus subtilis]
UniRef100_O31611

Bacillus subtilis

YjbM

1179

YjbN

1180
Hypothetical pseudouridine synthase
UniRef100_O31613

Bacillus subtilis

YjbO

yjbO [Bacillus subtilis]

1181
YjbP protein [Bacillus subtilis]
UniRef100_O31614

Bacillus subtilis

YjbP

1182
YjbQ protein [Bacillus subtilis]
UniRef100_O31615

Bacillus subtilis

YjbQ

1183
Transcriptional activator tenA [Bacillus
UniRef100_P25052

Bacillus subtilis

TenA

subtilis]

1184
Regulatory protein tenI [Bacillus
UniRef100_P25053

Bacillus subtilis

TenI

subtilis]

1185
Glycine oxidase [Bacillus subtilis]
UniRef100_O31616

Bacillus subtilis

GoxB

1186
ThiS protein [Bacillus subtilis]
UniRef100_O31617

Bacillus subtilis

1187
Thiazole biosynthesis protein thiG
UniRef100_O31618

Bacillus subtilis

ThiG

[Bacillus subtilis]

1188
ThiF protein [Bacillus subtilis]
UniRef100_O31619

Bacillus subtilis

ThiF

1189
YjbV protein [Bacillus subtilis]
UniRef100_O31620

Bacillus subtilis

YjbV

1190
Enoyl-[acyl-carrier-protein] reductase
UniRef100_P54616
acyl-carrier-
FabI

[NADH] [Bacillus subtilis]

protein

1191
YjbX protein [Bacillus subtilis]
UniRef100_O31622

Bacillus subtilis

YjbX

1192
Spore coat protein Z [Bacillus subtilis]
UniRef100_Q08312

Bacillus subtilis

CotZ

1193
Spore coat protein Y [Bacillus subtilis]
UniRef100_Q08311

Bacillus subtilis

CotY

1194
Spore coat protein X [Bacillus subtilis]
UniRef100_Q08313

Bacillus subtilis

CotX

1195
Spore coat protein W [Bacillus subtilis]
UniRef100_Q08310

Bacillus subtilis

CotW

1196
Spore coat protein V [Bacillus subtilis]
UniRef100_Q08309

Bacillus subtilis

CotV

1197
YjcA protein [Bacillus subtilis]
UniRef100_O31623

Bacillus subtilis

YjcA

1198

1199

1200
YjcC protein [Bacillus subtilis]
UniRef100_O31625

Bacillus subtilis

1201

YjcD

1202

YngC

1203

GalE

1204
YngB protein [Bacillus subtilis]
UniRef100_O31822

Bacillus subtilis

YngB

1205
YngA protein [Bacillus
UniRef100_Q70JY6

Bacillus

YngA

amyloliquefaciens]

amyloliquefaciens

1206
YjcF protein [Bacillus subtilis]
UniRef100_O31628

Bacillus subtilis

YjcF

1207
YjcG protein [Bacillus subtilis]
UniRef100_O31629

Bacillus subtilis

YjcG

1208
YjcH protein [Bacillus subtilis]
UniRef100_O31630

Bacillus subtilis

YjcH

1209
Hypothetical protein [Bacillus cereus]
UniRef100_Q739H9

Bacillus cereus

1210
BH1889 protein [Bacillus halodurans]
UniRef100_Q9KBN6

Bacillus

YobV

halodurans

1211
YjcI protein [Bacillus subtilis]
UniRef100_O31631

Bacillus subtilis

YjcI

1212
YjcJ protein [Bacillus subtilis]
UniRef100_O31632

Bacillus subtilis

YjcJ

1213
YjcL protein [Bacillus subtilis]
UniRef100_O31634

Bacillus subtilis

YjcL

1214
Transcriptional regulator, MarR/EmrR
UniRef100_Q97DR6

Clostridium acetobutylicum

family [Clostridium acetobutylicum]

1215
Penicillin-binding protein 4* [Bacillus
UniRef100_P32959

Bacillus subtilis

PbpE

subtilis]

1216

AbnA

1217

1218
Maltose transacetylase [Bacillus
UniRef100_Q75TH6

Bacillus

Maa

stearothermophilus]

stearothermophilus

1219

1220
Putative HTH-type transcriptional
UniRef100_P39647

Bacillus subtilis

YwfK

regulator ywfK [Bacillus subtilis]

1221
Sulfite reductase [Bacillus halodurans]
UniRef100_Q9KF76

Bacillus

YvgR

halodurans

1222
Sulfite reductase [Bacillus halodurans]
UniRef100_Q9KF75

Bacillus

YvgQ

halodurans

1223
Putative HTH-type transcriptional
UniRef100_O34701

Bacillus subtilis

YoaU

regulator yoaU [Bacillus subtilis]

1224
Hypothetical transport protein yoaV
UniRef100_O34416

Bacillus subtilis

YoaV

[Bacillus subtilis]

1225
Hypothetical protein VPA0302 [Vibrio
UniRef100_Q87JF1

Vibrio

YyaH

parahaemolyticus]

parahaemolyticus

1226
Hypothetical protein yoeB precursor
UniRef100_O34841

Bacillus subtilis

YoeB

[Bacillus subtilis]

1227

YocH

1228
Permease, general substrate
UniRef100_Q6HMC3

Bacillus

LmrB

transporter [Bacillus thuringiensis]

thuringiensis

1229
Putative HTH-type transcriptional
UniRef100_P42105

Bacillus subtilis

YxaF

regulator yxaF [Bacillus subtilis]

1230

1231

YmzD

1232

YeeF

1233

YjqB

1234
Phage-like element PBSX protein xkdA
UniRef100_P39780

Bacillus subtilis

XkdA

[Bacillus subtilis]

1235
HTH-type transcriptional regulator xre
UniRef100_P23789

Bacillus subtilis

Xre

[Bacillus subtilis]

1236

1237

1238
Phage-like element PBSX protein xkdB
UniRef100_P39781

Bacillus subtilis

XkdB

[Bacillus subtilis]

1239
Phage-like element PBSX protein xkdC
UniRef100_P39782

Bacillus subtilis

XkdC

[Bacillus subtilis]

1240
Phage-like element PBSX protein xkdD
UniRef100_P39783

Bacillus subtilis

XkdD

[Bacillus subtilis]

1241
Phage-like element PBSX protein xtrA
UniRef100_P54344

Bacillus subtilis

[Bacillus subtilis]

1242
Positive control factor [Bacillus subtilis]
UniRef100_P39784

Bacillus subtilis

Xpf

1243
PBSX phage terminase small subunit
UniRef100_P39785

Bacillus subtilis

XtmA

[Bacillus subtilis]

1244
PBSX phage terminase large subunit
UniRef100_P39786

Bacillus subtilis

XtmB

[Bacillus subtilis]

1245
Phage-like element PBSX protein xkdE
UniRef100_P54325

Bacillus subtilis

XkdE

[Bacillus subtilis]

1246
Phage-like element PBSX protein xkdF
UniRef100_P54326

Bacillus subtilis

XkdF

[Bacillus subtilis]

1247
Phage-like element PBSX protein xkdG
UniRef100_P54327

Bacillus subtilis

XkdG

[Bacillus subtilis]

1248
Hypothetical protein yqbG [Bacillus
UniRef100_P45923

Bacillus subtilis

YqbG

subtilis]

1249
Hypothetical protein yqbH [Bacillus
UniRef100_P45924

Bacillus subtilis

YqbH

subtilis]

1250
Phage-like element PBSX protein xkdI
UniRef100_P54329

Bacillus subtilis

XkdI

[Bacillus subtilis]

1251
Phage-like element PBSX protein xkdJ
UniRef100_P54330

Bacillus subtilis

XkdJ

[Bacillus subtilis]

1252
Lin1277 protein [Listeria innocua]
UniRef100_Q92CB2

Listeria innocua

1253
Phage-like element PBSX protein xkdK
UniRef100_P54331

Bacillus subtilis

XkdK

[Bacillus subtilis]

1254
Phage-like element PBSX protein xkdM
UniRef100_P54332

Bacillus subtilis

XkdM

[Bacillus subtilis]

1255
Phage-like element PBSX protein xkdN
UniRef100_P54333

Bacillus subtilis

XkdN

[Bacillus subtilis]

1256
Phage-like element PBSX protein xkdO
UniRef100_P54334

Bacillus subtilis

XkdO

[Bacillus subtilis]

1257
Phage-like element PBSX protein xkdP
UniRef100_P54335

Bacillus subtilis

YqbP

[Bacillus subtilis]

1258
Hypothetical protein yqbQ [Bacillus
UniRef100_P45950

Bacillus subtilis

YqbQ

subtilis]

1259
Hypothetical protein yqbR [Bacillus
UniRef100_P45933

Bacillus subtilis

YqbR

subtilis]

1260
Phage-like element PBSX protein xkdS
UniRef100_P54338

Bacillus subtilis

XkdS

[Bacillus subtilis]

1261
Hypothetical protein yqbT [Bacillus
UniRef100_P45935

Bacillus subtilis

YqbT

subtilis]

1262
Phage-like element PBSX protein xkdU
UniRef100_P54340

Bacillus subtilis

XkdU

[Bacillus subtilis]

1263

1264

XkdV

1265

YomR

1266

1267

1268

BlyA

1269
Regulatory protein [Bacillus
UniRef100_Q9ZFL9

Bacillus

YdhC

stearothermophilus]

stearothermophilus

1270
Zinc-containing alcohol dehydrogenase
UniRef100_O35045

Bacillus subtilis

YjmD

[Bacillus subtilis]

1271
Mannonate dehydratase 1 [Bacillus
UniRef100_Q9KDZ8

Bacillus

UxuA

halodurans]

halodurans

1272
D-mannonate oxidoreductase [Bacillus
UniRef100_Q9KDZ4

Bacillus

YjmF

halodurans]

halodurans

1273
UPI00002F2634 UniRef100 entry
UniRef100_UPI00002F2634

YjmD

1274
Hexuronate transporter [Bacillus
UniRef100_O34456

Bacillus subtilis

ExuT

subtilis]

1275
Stage II sporulation protein SB [Bacillus
UniRef100_O34800

Bacillus subtilis

subtilis]

1276
Stage II sporulation protein SA [Bacillus
UniRef100_O34853

Bacillus subtilis

SpoIISA

subtilis]

1277
UPI00003CC121 UniRef100 entry
UniRef100_UPI00003CC121

Pit

1278
Hypothetical UPF0111 protein ykaA
UniRef100_O34454

Bacillus subtilis

YkaA

[Bacillus subtilis]

1279

Ggt

1280
YesL protein [Bacillus subtilis]
UniRef100_O31515

Bacillus subtilis

YesL

1281
YesM protein [Bacillus subtilis]
UniRef100_O31516

Bacillus subtilis

YesM

1282
YesN protein [Bacillus subtilis]
UniRef100_O31517

Bacillus subtilis

YesN

1283
YesO protein [Bacillus subtilis]
UniRef100_O31518

Bacillus subtilis

YesO

1284
Probable ABC transporter permease
UniRef100_O31519

Bacillus subtilis

YesP

protein yesP [Bacillus subtilis]

1285
Probable ABC transporter permease
UniRef100_O31520

Bacillus subtilis

YesQ

protein yesQ [Bacillus subtilis]

1286
YesR protein [Bacillus subtilis]
UniRef100_O31521

Bacillus subtilis

YesR

1287
YesS protein [Bacillus subtilis]
UniRef100_O31522

Bacillus subtilis

YesS

1288
YesT protein [Bacillus subtilis]
UniRef100_O31523

Bacillus subtilis

YesT

1289

YesU

1290
YesV protein [Bacillus subtilis]
UniRef100_O31525

Bacillus subtilis

YesV

1291
YesW protein [Bacillus subtilis]
UniRef100_O31526

Bacillus subtilis

YesW

1292

1293

YesT

1294
YesX protein [Bacillus subtilis]
UniRef100_O31527

Bacillus subtilis

YesX

1295
Putative ion-channel protein
UniRef100_Q8Z4X6

Salmonella typhi

YccK

[Salmonella typhi]

1296
YesY protein [Bacillus subtilis]
UniRef100_O31528

Bacillus subtilis

YesY

1297
YesZ protein [Bacillus subtilis]
UniRef100_O31529

Bacillus subtilis

YesZ

1298

YetA

1299
Lipoprotein lplA precursor [Bacillus
UniRef100_P37966

Bacillus subtilis

LplA

subtilis]

1300
LplB protein [Bacillus subtilis]
UniRef100_P39128

Bacillus subtilis

LplB

1301
LplC protein [Bacillus subtilis]
UniRef100_P39129

Bacillus subtilis

LplC

1302
YkbA protein [Bacillus subtilis]
UniRef100_O34739

Bacillus subtilis

YkbA

1303
YkcA protein [Bacillus subtilis]
UniRef100_O34689

Bacillus subtilis

YkcA

1304
Hypothetical protein [Bacillus cereus]
UniRef100_Q81CP9

Bacillus cereus

1305
Probable serine protease do-like htrA
UniRef100_O34358

Bacillus subtilis

HtrA

[Bacillus subtilis]

1306
Pyrroline-5-carboxylate reductase 3
UniRef100_Q00777

Bacillus subtilis

ProG

[Bacillus subtilis]

1307
D-aminopeptidase [Bacillus subtilis]
UniRef100_P26902

Bacillus subtilis

DppA

1308
Dipeptide transport system permease
UniRef100_P26903

Bacillus subtilis

DppB

protein dppB [Bacillus subtilis]

1309
Dipeptide transport system permease
UniRef100_P26904

Bacillus subtilis

DppC

protein dppC [Bacillus subtilis]

1310
Dipeptide transport ATP-binding protein
UniRef100_P26905

Bacillus subtilis

DppD

dppD [Bacillus subtilis]

1311
Dipeptide-binding protein dppE
UniRef100_P26906

Bacillus subtilis

DppE

precursor [Bacillus subtilis]

1312
Hypothetical protein ykfA [Bacillus
UniRef100_O34851

Bacillus subtilis

YkfA

subtilis]

1313
YkfB [Bacillus subtilis]
UniRef100_O34508

Bacillus subtilis

YkfB

1314
YkfC [Bacillus subtilis]
UniRef100_O35010

Bacillus subtilis

YkfC

1315
YkfD [Bacillus subtilis]
UniRef100_O34480

Bacillus subtilis

YkfD

1316
BH1779 protein [Bacillus halodurans]
UniRef100_Q9KBZ5

Bacillus

YkgA

halodurans

1317
Putative acyl-CoA thioester hydrolase
UniRef100_P49851

Bacillus subtilis

YkhA

ykhA [Bacillus subtilis]

1318

YkjA

1319
Pectate lyase 47 precursor [Bacillus sp.
UniRef100_Q9AJM4

Bacillus sp. TS-
Pel

TS-47]

47

1320
Transcriptional regulator, PadR family
UniRef100_Q6HED5

Bacillus thuringiensis

[Bacillus thuringiensis]

1321
Hypothetical protein OB0568
UniRef100_Q8ESQ3

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

1322
BH1312 protein [Bacillus halodurans]
UniRef100_Q9KDA2

Bacillus halodurans

1323
Hypothetical protein ykkA [Bacillus
UniRef100_P49854

Bacillus subtilis

YkkA

subtilis]

1324
Hypothetical protein ykkC [Bacillus
UniRef100_P49856

Bacillus subtilis

YkkC

subtilis]

1325

1326
YkkE [Bacillus subtilis]
UniRef100_O34990

Bacillus subtilis

YkkE

1327
Glutamate 5-kinase 1 [Bacillus subtilis]
UniRef100_P39820

Bacillus subtilis

ProB

1328
Gamma-glutamyl phosphate reductase
UniRef100_P39821

Bacillus subtilis

ProA

[Bacillus subtilis]

1329
Organic hydroperoxide resistance
UniRef100_O34762

Bacillus subtilis

YklA

protein ohrA [Bacillus subtilis]

1330
Organic hydroperoxide resistance
UniRef100_O34777

Bacillus subtilis

YkmA

transcriptional regulator [Bacillus

subtilis]

1331
Organic hydroperoxide resistance
UniRef100_P80242

Bacillus subtilis

YkzA

protein ohrB [Bacillus subtilis]

1332

1333
Guanine deaminase [Bacillus subtilis]
UniRef100_O34598

Bacillus subtilis

GuaD

1334
Phosphoglycerate mutase [Bacillus
UniRef100_Q9ALU0

Bacillus

YhfR

stearothermophilus]

stearothermophilus

1335

1336

1337
5-methyltetrahydropteroyltriglutamate--
UniRef100_P80877

Bacillus subtilis

MetE

homocysteine methyltransferase

[Bacillus subtilis]

1338
Intracellular serine protease [Bacillus
UniRef100_Q69DB4

Bacillus sp.
IspA

sp. WRD-2]

WRD-2

1339

1340
YkoK [Bacillus subtilis]
UniRef100_O34442

Bacillus subtilis

YkoK

1341

1342
Integrase [Oceanobacillus iheyensis]
UniRef100_Q8ETV2

Oceanobacillus

YdcL

iheyensis

1343

YqaB

1344
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81UE0

Bacillus anthracis

1345

YonS

1346

1347
Putative HTH-type transcriptional
UniRef100_P45902

Bacillus subtilis

YqaE

regulator yqaE [Bacillus subtilis]

1348
Transcriptional regulator
UniRef100_Q7P886

Fusobacterium nucleatum

[Fusobacterium nucleatum subsp.

subsp. vincentii ATCC 49256

vincentii ATCC 49256]

1349

1350

1351
Lin1236 protein [Listeria innocua]
UniRef100_Q92CD6

Listeria innocua

1352

1353

1354

1355

YqaJ

1356
35 protein [Bacteriophage SPP1]
UniRef100_Q38143
Bacteriophage
YqaK

SPP1

1357

YqaL

1358

YqaM

1359
Hypothetical protein yqaO [Bacillus
UniRef100_P45912

Bacillus subtilis

subtilis]

1360
Hypothetical protein yopY
UniRef100_O64108
Bacteriophage SPBc2

[Bacteriophage SPBc2]

1361

1362

1363

1364
Hypothetical protein CTC02137
UniRef100_Q892G2

Clostridium tetani

[Clostridium tetani]

1365
Hypothetical protein MW1918
UniRef100_Q8NVN5

Staphylococcus

YqaN

[Staphylococcus aureus]

aureus

1366

1367
Single-strand binding protein 2 [Listeria
UniRef100_Q8Y4X1

Listeria monocytogenes

monocytogenes]

1368

1369

1370
Hypothetical protein yqaQ [Bacillus
UniRef100_P45948

Bacillus subtilis

YqaQ

subtilis]

1371

1372
Hypothetical protein yqaS [Bacillus
UniRef100_P45915

Bacillus subtilis

YqaS

subtilis]

1373
Hypothetical protein yqaT [Bacillus
UniRef100_P45916

Bacillus subtilis

YqaT

subtilis]

1374
Hypothetical phage associated protein
UniRef100_Q8K610

Streptococcus pyogenes

SpyM3_1326 [Streptococcus pyogenes]

1375
Minor head structural component GP7
UniRef100_Q38442
Bacteriophage SPP1

[Bacteriophage SPP1]

1376

1377

1378

1379
Hypothetical protein CTC01553
UniRef100_Q894J0

Clostridium tetani

[Clostridium tetani]

1380
Major capsid protein [Bacteriophage
UniRef100_Q9T1B7
Bacteriophage A118

A118]

1381
ORF28 [Bacteriophage phi-105]
UniRef100_Q9ZXF5
Bacteriophage phi-105

1382

1383
15 protein [Bacteriophage SPP1]
UniRef100_Q38584
Bacteriophage SPP1

1384
Complete nucleotide sequence
UniRef100_O48446
Bacteriophage SPP1

[Bacteriophage SPP1]

1385

1386
Complete nucleotide sequence
UniRef100_O48448
Bacteriophage SPP1

[Bacteriophage SPP1]

1387
Complete nucleotide sequence
UniRef100_O48449
Bacteriophage SPP1

[Bacteriophage SPP1]

1388

1389
Complete nucleotide sequence
UniRef100_O48453
Bacteriophage SPP1

[Bacteriophage SPP1]

1390

1391
Complete nucleotide sequence
UniRef100_O48455
Bacteriophage
XkdO

[Bacteriophage SPP1]

SPP1

1392
Complete nucleotide sequence
UniRef100_O48459
Bacteriophage SPP1

[Bacteriophage SPP1]

1393
Complete nucleotide sequence
UniRef100_O48463
Bacteriophage SPP1

[Bacteriophage SPP1]

1394

1395

1396
LycA [Clostridium botulinum]
UniRef100_Q6R100

Clostridium botulinum

1397
Hypothetical protein yrkC [Bacillus
UniRef100_P54430

Bacillus subtilis

YrkC

subtilis]

1398

YhjR

1399
YdfS protein [Bacillus subtilis]
UniRef100_P96697

Bacillus subtilis

YdfS

1400
HTH-type transcriptional regulator tnrA
UniRef100_Q45666

Bacillus subtilis

TnrA

[Bacillus subtilis]

1401
Hypothetical protein ykzB [Bacillus
UniRef100_O34923

Bacillus subtilis

subtilis]

1402

1403
YkoM [Bacillus subtilis]
UniRef100_O34949

Bacillus subtilis

YkoM

1404
YkoU protein [Bacillus subtilis]
UniRef100_O34398

Bacillus subtilis

YkoU

1405
YkoV protein [Bacillus subtilis]
UniRef100_O34859

Bacillus subtilis

YkoV

1406
Signaling protein ykoW [Bacillus
UniRef100_O34311

Bacillus subtilis

YkoW

subtilis]

1407
YkoX protein [Bacillus subtilis]
UniRef100_O34908

Bacillus subtilis

YkoX

1408
YkoY protein [Bacillus subtilis]
UniRef100_O34997

Bacillus subtilis

YkoY

1409
RNA polymerase sigma factor [Bacillus
UniRef100_O31654

Bacillus subtilis

SigI

subtilis]

1410

YkrI

1411
Small, acid-soluble spore protein C3
UniRef100_P10572

Bacillus megaterium

[Bacillus megaterium]

1412
YkrK protein [Bacillus subtilis]
UniRef100_O31656

Bacillus subtilis

YkrK

1413
Probable protease htpX homolog
UniRef100_O31657

Bacillus subtilis

YkrL

[Bacillus subtilis]

1414
YkrM protein [Bacillus subtilis]
UniRef100_O31658

Bacillus subtilis

YkrM

1415
Penicillin-binding protein 3 [Bacillus
UniRef100_P42971

Bacillus subtilis

PbpC

subtilis]

1416
Hypothetical protein [Bacillus cereus]
UniRef100_Q73BI4

Bacillus cereus

1417

1418
YkrP protein [Bacillus subtilis]
UniRef100_O31660

Bacillus subtilis

YkrP

1419
Two-component sensor histidine kinase
UniRef100_O31661

Bacillus subtilis

KinE

[Bacillus subtilis]

1420
Methylated-DNA--protein-cysteine
UniRef100_P11742

Bacillus subtilis

Ogt

methyltransferase [Bacillus subtilis]

1421

1422
Methylthioribose-1-phosphate
UniRef100_O31662

Bacillus subtilis

YkrS

isomerase [Bacillus subtilis]

1423
Methylthioribose kinase [Bacillus
UniRef100_O31663

Bacillus subtilis

YkrT

subtilis]

1424
YkrU protein [Bacillus subtilis]
UniRef100_O31664

Bacillus subtilis

YkrU

1425
Transaminase mtnE [Bacillus subtilis]
UniRef100_O31665

Bacillus subtilis

YkrV

1426
2,3-diketo-5-methylthiopentyl-1-
UniRef100_O31666

Bacillus subtilis

YkrW

phosphate enolase [Bacillus subtilis]

1427
Methylthioribulose-1-phosphate
UniRef100_O31668

Bacillus subtilis

YkrY

dehydratase [Bacillus subtilis]

1428
1,2-dihydroxy-3-keto-5-
UniRef100_O31669

Bacillus subtilis

YkrZ

methylthiopentene dioxygenase

[Bacillus subtilis]

1429
Metallothiol transferase fosB
UniRef100_Q8CXK5

Oceanobacillus

YndN

[Oceanobacillus iheyensis]

iheyensis

1430
YkvA protein [Bacillus subtilis]
UniRef100_O31670

Bacillus subtilis

1431
Stage 0 sporulation regulatory protein
UniRef100_P05043

Bacillus subtilis

[Bacillus subtilis]

1432
Two-component sensor histidine kinase
UniRef100_O31671

Bacillus subtilis

KinD

[Bacillus subtilis]

1433

YkvE

1434
Chemotaxis motB protein [Bacillus
UniRef100_P28612

Bacillus subtilis

MotB

subtilis]

1435
Chemotaxis motA protein [Bacillus
UniRef100_P28611

Bacillus subtilis

MotA

subtilis]

1436
ATP-dependent Clp protease-like
UniRef100_O31673

Bacillus subtilis

ClpE

[Bacillus subtilis]

1437
YkvI protein [Bacillus subtilis]
UniRef100_O31674

Bacillus subtilis

YkvI

1438
YkvJ protein [Bacillus subtilis]
UniRef100_O31675

Bacillus subtilis

YkvJ

1439
YkvK protein [Bacillus subtilis]
UniRef100_O31676

Bacillus subtilis

YkvK

1440
YkvL protein [Bacillus subtilis]
UniRef100_O31677

Bacillus subtilis

YkvL

1441
YkvM protein [Bacillus subtilis]
UniRef100_O31678

Bacillus subtilis

YkvM

1442
DNA integration/recombination protein
UniRef100_Q894H7

Clostridium tetani

CodV

[Clostridium tetani]

1443
Integrase/recombinase [Bacillus cereus
UniRef100_Q633V7

Bacillus cereus

RipX

ZK]

ZK

1444

1445

1446

1447
YoqV protein [Bacteriophage SPBc2]
UniRef100_O64130
Bacteriophage
LigB

SPBc2

1448

1449

1450
UPI00003CC586 UniRef100 entry
UniRef100_UPI00003CC586

1451

1452

1453

1454

1455
Prophage LambdaBa02, HNH
UniRef100_Q81W86

Bacillus anthracis

endonuclease family protein [Bacillus

anthracis]

1456
Terminase small subunit
UniRef100_Q6GAL5

Staphylococcus aureus

[Staphylococcus aureus]

1457
Prophage LambdaBa02, terminase,
UniRef100_Q6HUD2

Bacillus anthracis

large subunit, putative [Bacillus

anthracis]

1458
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81W89

Bacillus anthracis

1459
ClpP family serine protease, possible
UniRef100_Q97HW4

Clostridium

ClpP

phage related [Clostridium

acetobutylicum

acetobutylicum]

1460
Prophage LambdaBa02, major capsid
UniRef100_Q81W91

Bacillus anthracis

protein, putative [Bacillus anthracis]

1461
Precursor polypeptide (AA −37 to 1647)
UniRef100_Q03658
unidentified bacterium

precursor [unidentified bacterium]

1462
Gp7 protein [Bacteriophage phi3626]
UniRef100_Q8SBP7
Bacteriophage phi3626

1463
Uncharacterized phage related protein
UniRef100_Q97HW7

Clostridium acetobutylicum

[Clostridium acetobutylicum]

1464
Hypothetical protein CAC1887
UniRef100_Q97HW9

Clostridium acetobutylicum

[Clostridium acetobutylicum]

1465
Prophage LambdaBa02, major tail
UniRef100_Q81W97

Bacillus anthracis

protein, putative [Bacillus anthracis]

1466

1467

1468
Prophage LambdaBa02, tape measure
UniRef100_Q81WA0

Bacillus

YqbO

protein, putative [Bacillus anthracis]

anthracis

1469
Lin2382 protein [Listeria innocua]
UniRef100_Q928Z8

Listeria innocua

1470
Protein gp18 [Listeria monocytogenes]
UniRef100_Q8Y4Z4

Listeria monocytogenes

1471

YclG

1472

XkdV

1473

XkdW

1474
YomP protein [Bacteriophage SPBc2]
UniRef100_O64052
Bacteriophage SPBc2

1475
Glycerophosphoryl diester
UniRef100_Q737E6

Bacillus cereus

GlpQ

phosphodiesterase, putative [Bacillus

cereus]

1476
Protein bhlA [Bacteriophage SPBc2]
UniRef100_O64039
Bacteriophage SPBc2

1477
ORF46 [Bacteriophage phi-105]
UniRef100_Q9ZXD7
Bacteriophage
XlyB

phi-105

1478

1479

1480

1481
Transcriptional regulator, DeoR family
UniRef100_Q816D5

Bacillus cereus

[Bacillus cereus]

1482
Hypothetical protein yolD
UniRef100_O64030
Bacteriophage SPBc2

[Bacteriophage SPBc2]

1483
DNA integration/recombination/invertion
UniRef100_Q81GD4

Bacillus cereus

YdcL

protein [Bacillus cereus]

1484
YkvM protein [Bacillus subtilis]
UniRef100_O31678

Bacillus subtilis

1485
Response regulator aspartate
UniRef100_P40771

Bacillus subtilis

RapH

phosphatase H [Bacillus subtilis]

1486
YoaT [Bacillus subtilis]
UniRef100_O34535

Bacillus subtilis

YoaT

1487
YozG protein [Bacillus subtilis]
UniRef100_O31834

Bacillus subtilis

1488
YoaS protein [Bacillus subtilis]
UniRef100_O31833

Bacillus subtilis

YoaS

1489

1490

1491

1492

1493

1494
YkvS protein [Bacillus subtilis]
UniRef100_O31684

Bacillus subtilis

1495
BH2327 protein [Bacillus halodurans]
UniRef100_Q9KAG0

Bacillus halodurans

1496
YkvT protein [Bacillus subtilis]
UniRef100_O31685

Bacillus subtilis

YkvT

1497
YkvU protein [Bacillus subtilis]
UniRef100_O31686

Bacillus subtilis

YkvU

1498
YkvV protein [Bacillus subtilis]
UniRef100_O31687

Bacillus subtilis

YkvV

1499

YkvW

1500
YkvY protein [Bacillus subtilis]
UniRef100_O31689

Bacillus subtilis

YkvY

1501
Necrosis and ethylene inducing protein
UniRef100_Q9KFT2

Bacillus halodurans

[Bacillus halodurans]

1502
Putative HTH-type transcriptional
UniRef100_O31690

Bacillus subtilis

YkvZ

regulator ykvZ [Bacillus subtilis]

1503
Transcription antiterminator [Bacillus
UniRef100_O06710

Bacillus subtilis

GlcT

subtilis]

1504

PtsG

1505
Phosphocarrier protein HPr [Bacillus
UniRef100_P08877

Bacillus subtilis

subtilis]

1506
Phosphoenolpyruvate-protein
UniRef100_P08838

Bacillus subtilis

PtsI

phosphotransferase [Bacillus subtilis]

1507
SplA [Bacillus amyloliquefaciens]
UniRef100_O54358

Bacillus amyloliquefaciens

1508
Spore photoproduct lyase [Bacillus
UniRef100_O54359

Bacillus

SplB

amyloliquefaciens]

amyloliquefaciens

1509
Hypothetical protein orf1 [Bacillus
UniRef100_O05187

Bacillus subtilis

YkwB

subtilis]

1510
Methyl-accepting chemotaxis protein
UniRef100_P54576

Bacillus subtilis

McpC

mcpC [Bacillus subtilis]

1511
3-oxoacyl-(Acyl carrier protein)
UniRef100_Q8EMP9

Oceanobacillus

FabG

reductase [Oceanobacillus iheyensis]

iheyensis

1512
Hypothetical oxidoreductase ykwC
UniRef100_O34948

Bacillus subtilis

YkwC

[Bacillus subtilis]

1513
YkwD protein [Bacillus subtilis]
UniRef100_O31694

Bacillus subtilis

YkwD

1514
YkuA protein [Bacillus subtilis]
UniRef100_O31399

Bacillus subtilis

YkuA

1515
Sporulation kinase A [Bacillus subtilis]
UniRef100_P16497

Bacillus subtilis

KinA

1516
Putative aminotransferase A [Bacillus
UniRef100_P16524

Bacillus subtilis

PatA

subtilis]

1517

1518
Hypothetical protein yxaI [Bacillus
UniRef100_P42108

Bacillus subtilis

YxaI

subtilis]

1519

YxiO

1520
Chemotaxis protein cheV [Bacillus
UniRef100_P37599

Bacillus subtilis

CheV

subtilis]

1521
Hypothetical protein ykyB [Bacillus
UniRef100_P42430

Bacillus subtilis

YkyB

subtilis]

1522

YkuC

1523
YkuD protein [Bacillus subtilis]
UniRef100_O34816

Bacillus subtilis

YkuD

1524

YkuE

1525
Hypothetical oxidoreductase ykuF
UniRef100_O34717

Bacillus subtilis

YkuF

[Bacillus subtilis]

1526
YkuI protein [Bacillus subtilis]
UniRef100_O35014

Bacillus subtilis

YkuI

1527

1528
YkuJ protein [Bacillus subtilis]
UniRef100_O34588

Bacillus subtilis

1529
YkuK protein [Bacillus subtilis]
UniRef100_O34776

Bacillus subtilis

YkuK

1530
Hypothetical protein ykzF [Bacillus
UniRef100_O31697

Bacillus subtilis

subtilis]

1531
YkuL protein [Bacillus subtilis]
UniRef100_O31698

Bacillus subtilis

YkuL

1532
Putative HTH-type transcriptional
UniRef100_O34827

Bacillus subtilis

CcpC

regulator ykuM [Bacillus subtilis]

1533
Probable flavodoxin 1 [Bacillus subtilis]
UniRef100_O34737

Bacillus subtilis

YkuN

1534
YkuO protein [Bacillus subtilis]
UniRef100_O34879

Bacillus subtilis

YkuO

1535
Probable flavodoxin 2 [Bacillus subtilis]
UniRef100_O34589

Bacillus subtilis

YkuP

1536
YkuQ protein [Bacillus subtilis]
UniRef100_O34981

Bacillus subtilis

YkuQ

1537
YkuR protein [Bacillus subtilis]
UniRef100_O34916

Bacillus subtilis

YkuR

1538
Hypothetical UPF0180 protein ykuS
UniRef100_O34783

Bacillus subtilis

[Bacillus subtilis]

1539
YkuU protein [Bacillus subtilis]
UniRef100_O34564

Bacillus subtilis

YkuU

1540
YkuV protein [Bacillus subtilis]
UniRef100_O31403

Bacillus subtilis

YkuV

1541
Repressor rok [Bacillus subtilis]
UniRef100_O34857

Bacillus subtilis

Rok

1542
YknT protein [Bacillus subtilis]
UniRef100_O31700

Bacillus subtilis

YknT

1543

MobA

1544
Molybdopterin biosynthesis protein
UniRef100_O31702

Bacillus subtilis

MoeB

MoeB [Bacillus subtilis]

1545
Molybdopterin biosynthesis protein
UniRef100_O31703

Bacillus subtilis

MoeA

MoeA [Bacillus subtilis]

1546
Molybdopterin-guanine dinucleotide
UniRef100_O31704

Bacillus subtilis

MobB

biosynthesis protein B [Bacillus subtilis]

1547
Molybdopterin converting factor subunit
UniRef100_O31705

Bacillus subtilis

MoaE

2 [Bacillus subtilis]

1548
Molybdopterin converting factor,
UniRef100_O31706

Bacillus subtilis

subunit 1 [Bacillus subtilis]

1549

1550
YknU protein [Bacillus subtilis]
UniRef100_O31707

Bacillus subtilis

YknU

1551
YknV protein [Bacillus subtilis]
UniRef100_O31708

Bacillus subtilis

YknV

1552
Hypothetical protein yknW [Bacillus
UniRef100_O31709

Bacillus subtilis

YknW

subtilis]

1553
YknX protein [Bacillus subtilis]
UniRef100_O31710

Bacillus subtilis

YknX

1554
YknY protein [Bacillus subtilis]
UniRef100_O31711

Bacillus subtilis

YknY

1555
Hypothetical protein yknZ [Bacillus
UniRef100_O31712

Bacillus subtilis

YknZ

subtilis]

1556

FruR

1557
1-phosphofructokinase [Bacillus
UniRef100_O31714

Bacillus subtilis

FruK

subtilis]

1558
Phosphotransferase system (PTS)
UniRef100_P71012

Bacillus subtilis

FruA

fructose-specific enzyme IIABC

component [Bacillus subtilis]

1559
Signal peptidase I T [Bacillus subtilis]
UniRef100_P71013

Bacillus subtilis

SipT

1560
Hypothetical protein ykoA [Bacillus
UniRef100_O31715

Bacillus subtilis

subtilis]

1561

1562
YkpA protein [Bacillus subtilis]
UniRef100_O31716

Bacillus subtilis

YkpA

1563
BH1921 protein [Bacillus halodurans]
UniRef100_Q9KBK5

Bacillus halodurans

1564
Aminopeptidase ampS [Bacillus subtilis]
UniRef100_P39762

Bacillus subtilis

AmpS

1565

1566
MreBH protein [Bacillus subtilis]
UniRef100_P39763

Bacillus subtilis

MreBH

1567

1568
Sporulation kinase C [Bacillus subtilis]
UniRef100_P39764

Bacillus subtilis

KinC

1569
Hypothetical protein ykqB [Bacillus
UniRef100_P39760

Bacillus subtilis

YkqB

subtilis]

1570
Adenine deaminase [Bacillus subtilis]
UniRef100_P39761

Bacillus subtilis

AdeC

1571

YkqC

1572
YkzG protein [Bacillus subtilis]
UniRef100_O31718

Bacillus subtilis

1573
Hypothetical protein ykrA [Bacillus
UniRef100_Q45494

Bacillus subtilis

YkrA

subtilis]

1574

YkrB

1575

1576
Hypothetical protein ykyA [Bacillus
UniRef100_P21884

Bacillus subtilis

YkyA

subtilis]

1577
Pyruvate dehydrogenase E1
UniRef100_P21881

Bacillus subtilis

PdhA

component, alpha subunit [Bacillus

subtilis]

1578
Pyruvate dehydrogenase E1
UniRef100_P21882

Bacillus subtilis

PdhB

component, beta subunit [Bacillus

subtilis]

1579
Dihydrolipoyllysine-residue
UniRef100_P21883

Bacillus subtilis

PdhC

acetyltransferase component of

pyruvate dehydrogenase complex

[Bacillus subtilis]

1580
Dihydrolipoyl dehydrogenase [Bacillus
UniRef100_P21880

Bacillus subtilis

PdhD

subtilis]

1581
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069

1582
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012

anthracis str. A2012]

1583

1584

1585
Arginine decarboxylase [Bacillus
UniRef100_P21885

Bacillus subtilis

SpeA

subtilis]

1586
Hypothetical UPF0223 protein yktA
UniRef100_Q45497

Bacillus subtilis

[Bacillus subtilis]

1587
Hypothetical protein yktB [Bacillus
UniRef100_Q45498

Bacillus subtilis

YktB

subtilis]

1588
YkzI protein [Bacillus subtilis]
UniRef100_O31719

Bacillus subtilis

1589
Inositol-1-monophosphatase [Bacillus
UniRef100_Q45499

Bacillus subtilis

YktC

subtilis]

1590
Hypothetical protein ykzC [Bacillus
UniRef100_O31720

Bacillus subtilis

YkzC

subtilis]

1591
Hypothetical protein ylaA [Bacillus
UniRef100_O07625

Bacillus subtilis

YlaA

subtilis]

1592
Hypothetical protein ylaB [Bacillus
UniRef100_O07626

Bacillus subtilis

subtilis]

1593
YlaC protein [Bacillus subtilis]
UniRef100_O07627

Bacillus subtilis

YlaC

1594
Hypothetical protein ylaD [Bacillus
UniRef100_O07628

Bacillus subtilis

subtilis]

1595
Hypothetical protein ylaF [Bacillus
UniRef100_O07630

Bacillus subtilis

subtilis]

1596
GTP-binding protein typA/bipA homolog
UniRef100_O07631

Bacillus subtilis

YlaG

[Bacillus subtilis]

1597
YlaH protein [Bacillus subtilis]
UniRef100_O07632

Bacillus subtilis

YlaH

1598
YhzA homolog [Bacillus subtilis]
UniRef100_O07562

Bacillus subtilis

YhjH

1599
Hypothetical protein yhjG [Bacillus
UniRef100_O07561

Bacillus subtilis

YhjG

subtilis]

1600

1601
Hypothetical lipoprotein ylaJ precursor
UniRef100_O07634

Bacillus subtilis

YlaJ

[Bacillus subtilis]

1602
YlaK protein [Bacillus subtilis]
UniRef100_O07635

Bacillus subtilis

YlaK

1603
UPI00003CB7B1 UniRef100 entry
UniRef100_UPI00003CB7B1

YlaL

1604
Probable glutaminase ylaM [Bacillus
UniRef100_O07637

Bacillus subtilis

YlaM

subtilis]

1605
YlaN protein [Bacillus subtilis]
UniRef100_O07638

Bacillus subtilis

1606
Hypothetical protein ylaO [Bacillus
UniRef100_O07639

Bacillus subtilis

FtsW

subtilis]

1607

PycA

1608
Cytochrome AA3 controlling protein
UniRef100_P12946

Bacillus subtilis

CtaA

[Bacillus subtilis]

1609
Protoheme IX farnesyltransferase
UniRef100_P24009

Bacillus subtilis

CtaB

[Bacillus subtilis]

1610
Cytochrome c oxidase polypeptide II
UniRef100_P24011

Bacillus subtilis

CtaC

precursor (EC 1.9.3.1) (Cytochrome

aa3 subunit 2) (Caa-3605 subunit 2)

(Oxidase aa(3) subunit 2) [Bacillus

subtilis]

1611
Cytochrome c oxidase polypeptide I
UniRef100_P24010

Bacillus subtilis

CtaD

(EC 1.9.3.1) (Cytochrome aa3 subunit

1) (Caa-3605 subunit 1) (Oxidase aa(3)

subunit 1) [Bacillus subtilis]

1612
Cytochrome c oxidase polypeptide III
UniRef100_P24012

Bacillus subtilis

CtaE

(EC 1.9.3.1) (Cytochrome aa3 subunit

3) (Caa-3605 subunit 3) (Oxidase aa(3)

subunit 3) [Bacillus subtilis]

1613
Cytochrome c oxidase polypeptide IVB
UniRef100_P24013

Bacillus subtilis

CtaF

[Bacillus subtilis]

1614
CtaG protein [Bacillus subtilis]
UniRef100_O34329

Bacillus subtilis

CtaG

1615
YlbA protein [Bacillus subtilis]
UniRef100_O34743

Bacillus subtilis

YlbA

1616
YlbB protein [Bacillus subtilis]
UniRef100_O34682

Bacillus subtilis

YlbB

1617
YlbC protein [Bacillus subtilis]
UniRef100_O34586

Bacillus subtilis

YlbC

1618
YlbD protein [Bacillus subtilis]
UniRef100_O34880

Bacillus subtilis

YlbD

1619
YlbE protein [Bacillus subtilis]
UniRef100_O34958

Bacillus subtilis

1620
Regulatory protein ylbF [Bacillus
UniRef100_O34412

Bacillus subtilis

YlbF

subtilis]

1621
Hypothetical UPF0298 protein ylbG
UniRef100_O34658

Bacillus subtilis

[Bacillus subtilis]

1622
YlbH protein [Bacillus subtilis]
UniRef100_O34331

Bacillus subtilis

YlbH

1623
Phosphopantetheine
UniRef100_O34797

Bacillus subtilis

YlbI

adenylyltransferase [Bacillus subtilis]

1624

YlbJ

1625
YlbL protein [Bacillus subtilis]
UniRef100_O34470

Bacillus subtilis

YlbL

1626
YlbM protein [Bacillus subtilis]
UniRef100_O34513

Bacillus subtilis

YlbM

1627
YlbN protein [Bacillus subtilis]
UniRef100_O34445

Bacillus subtilis

YlbN

1628
50S ribosomal protein L32 [Bacillus
UniRef100_O34687

Bacillus subtilis

subtilis]

1629
Hypothetical protein ylbO [Bacillus
UniRef100_O34549

Bacillus subtilis

YlbO

subtilis]

1630
YlbP protein [Bacillus subtilis]
UniRef100_O34468

Bacillus subtilis

YlbP

1631
Probable 2-dehydropantoate 2-
UniRef100_O34661

Bacillus subtilis

YlbQ

reductase [Bacillus subtilis]

1632

YllA

1633
Protein mraZ [Bacillus subtilis]
UniRef100_P55343

Bacillus subtilis

YllB

1634
S-adenosyl-methyltransferase mraW
UniRef100_Q07876

Bacillus subtilis

YlxA

[Bacillus subtilis]

1635

FtsL

1636
Penicillin-binding protein 2B [Bacillus
UniRef100_Q07868

Bacillus subtilis

PbpB

subtilis]

1637
Stage V sporulation protein D [Bacillus
UniRef100_Q03524

Bacillus subtilis

SpoVD

subtilis]

1638
UDP-N-acetylmuramoylalanyl-D-
UniRef100_Q03523

Bacillus subtilis

MurE

glutamate--2,6-diaminopimelate ligase

[Bacillus subtilis]

1639
Phospho-N-acetylmuramoyl-
UniRef100_Q03521

Bacillus subtilis

MraY

pentapeptide-transferase [Bacillus

subtilis]

1640
UDP-N-acetylmuramoylalanine--D-
UniRef100_Q03522

Bacillus subtilis

MurD

glutamate ligase [Bacillus subtilis]

1641
Stage V sporulation protein E [Bacillus
UniRef100_P07373

Bacillus subtilis

SpoVE

subtilis]

1642
UDP-N-acetylglucosamine--N-
UniRef100_P37585

Bacillus subtilis

MurG

acetylmuramyl-(pentapeptide)pyrophosphoryl-

undecaprenol N-

acetylglucosamine transferase [Bacillus

subtilis]

1643
UDP-N-acetylenolpyruvoylglucosamine
UniRef100_P18579

Bacillus subtilis

MurB

reductase [Bacillus subtilis]

1644

DivIB

1645

YlxW

1646

YlxX

1647

Sbp

1648

FtsA

1649
Cell division protein ftsZ [Bacillus
UniRef100_P17865

Bacillus subtilis

FtsZ

subtilis]

1650

Bpr

1651
Bacillopeptidase F precursor [Bacillus
UniRef100_P16397

Bacillus subtilis

Bpr

subtilis]

1652
Sporulation sigma-E factor processing
UniRef100_P13801

Bacillus subtilis

SpoIIGA

peptidase [Bacillus subtilis]

1653
RNA polymerase sigma-E factor
UniRef100_P06222

Bacillus subtilis

SigE

precursor [Bacillus subtilis]

1654
RNA polymerase sigma-G factor
UniRef100_P19940

Bacillus subtilis

SigG

[Bacillus subtilis]

1655
YlmA protein [Bacillus subtilis]
UniRef100_O31723

Bacillus subtilis

YlmA

1656

1657
YlmC protein [Bacillus subtilis]
UniRef100_O31725

Bacillus subtilis

1658
Hypothetical UPF0124 protein ylmD
UniRef100_O31726

Bacillus subtilis

YlmD

[Bacillus subtilis]

1659

YlmE

1660
YlmF protein [Bacillus subtilis]
UniRef100_O31728

Bacillus subtilis

YlmF

1661
YlmG protein [Bacillus subtilis]
UniRef100_O31729

Bacillus subtilis

1662
Minicell-associated protein [Bacillus
UniRef100_P71020

Bacillus subtilis

YlmH

subtilis]

1663
Minicell-associated protein DivIVA
UniRef100_P71021

Bacillus subtilis

DivIVA

[Bacillus subtilis]

1664
Isoleucyl-tRNA synthetase [Bacillus
UniRef100_Q45477

Bacillus subtilis

IleS

subtilis]

1665

YlyA

1666

LspA

1667
Hypothetical pseudouridine synthase
UniRef100_Q45480

Bacillus subtilis

YlyB

ylyB [Bacillus subtilis]

1668
PyrR bifunctional protein [Includes:
UniRef100_P39765
Includes:
PyrR

Pyrimidine operon regulatory protein;

Pyrimidine

Uracil phosphoribosyltransferase (EC

operon

2.4.2.9) (UPRTase)] [Bacillus subtilis]

regulatory

protein; Uracil

phosphoribosyltransferase

(EC

2.4.2.9)

(UPRTase)

1669
Uracil permease [Bacillus subtilis]
UniRef100_P39766

Bacillus subtilis

PyrP

1670
Aspartate carbamoyltransferase
UniRef100_P05654

Bacillus subtilis

PyrB

[Bacillus subtilis]

1671
Dihydroorotase [Bacillus subtilis]
UniRef100_P25995

Bacillus subtilis

PyrC

1672
Carbamoyl-phosphate synthase,
UniRef100_P25993

Bacillus subtilis

PyrAA

pyrimidine-specific, small chain

[Bacillus subtilis]

1673
Carbamoyl-phosphate synthase,
UniRef100_P25994

Bacillus subtilis

PyrAB

pyrimidine-specific, large chain [Bacillus

subtilis]

1674
Dihydroorotate dehydrogenase electron
UniRef100_P25983

Bacillus subtilis

PyrK

transfer subunit [Bacillus subtilis]

1675
Dihydroorotate dehydrogenase,
UniRef100_P25996

Bacillus subtilis

PyrD

catalytic subunit [Bacillus subtilis]

1676
Orotidine 5′-phosphate decarboxylase
UniRef100_P25971

Bacillus subtilis

PyrF

[Bacillus subtilis]

1677
Orotate phosphoribosyltransferase
UniRef100_P25972

Bacillus subtilis

PyrE

[Bacillus subtilis]

1678

1679

CysH

1680
YlnA protein [Bacillus subtilis]
UniRef100_O34734

Bacillus subtilis

CysP

1681
Sulfate adenylyltransferase [Bacillus
UniRef100_O34764

Bacillus subtilis

Sat

subtilis]

1682
Probable adenylyl-sulfate kinase
UniRef100_O34577

Bacillus subtilis

CysC

[Bacillus subtilis]

1683
Putative S-adenosyl L-methionine:
UniRef100_O34744

Bacillus subtilis

YlnD

uroporphyrinogen III methyltransferase

[Bacillus subtilis]

1684
Sirohydrochlorin ferrochelatase
UniRef100_O34632

Bacillus subtilis

YlnE

[Bacillus subtilis]

1685
YlnF protein [Bacillus subtilis]
UniRef100_O34813

Bacillus subtilis

YlnF

1686
Putative fibronectin-binding protein
UniRef100_O34693

Bacillus subtilis

YloA

[Bacillus subtilis]

1687
YloB protein [Bacillus subtilis]
UniRef100_O34431

Bacillus subtilis

YloB

1688
YloC protein [Bacillus subtilis]
UniRef100_O34441

Bacillus subtilis

YloC

1689
Hypothetical UPF0296 protein ylzA
UniRef100_Q7WY72

Bacillus subtilis

[Bacillus subtilis]

1690
Guanylate kinase [Bacillus subtilis]
UniRef100_O34328

Bacillus subtilis

Gmk

1691
DNA-directed RNA polymerase omega
UniRef100_O35011

Bacillus subtilis

chain [Bacillus subtilis]

1692
YloI protein [Bacillus subtilis]
UniRef100_O35033

Bacillus subtilis

YloI

1693
Primosomal protein N′ [Bacillus subtilis]
UniRef100_P94461

Bacillus subtilis

PriA

1694
Peptide deformylase 1 [Bacillus subtilis]
UniRef100_P94462

Bacillus subtilis

Def

1695
Methionyl-tRNA formyltransferase
UniRef100_P94463

Bacillus subtilis

Fmt

[Bacillus subtilis]

1696
Ribosomal RNA small subunit
UniRef100_P94464

Bacillus subtilis

YloM

methyltransferase B (EC 2.1.1.—) (rRNA

(cytosine-C(5)-)-methyltransferase)

[Bacillus subtilis]

1697
Hypothetical UPF0063 protein yloN
UniRef100_O34617

Bacillus subtilis

YloN

[Bacillus subtilis]

1698
Protein phosphatase [Bacillus subtilis]
UniRef100_O34779

Bacillus subtilis

PrpC

1699
Probable serine/threonine-protein
UniRef100_O34507

Bacillus subtilis

PrkC

kinase yloP [Bacillus subtilis]

1700
Probable GTPase engC [Bacillus
UniRef100_O34530

Bacillus subtilis

YloQ

subtilis]

1701
Ribulose-phosphate 3-epimerase
UniRef100_O34557

Bacillus subtilis

Rpe

[Bacillus subtilis]

1702
YloS protein [Bacillus subtilis]
UniRef100_O34664

Bacillus subtilis

YloS

1703

1704
50S ribosomal protein L28 [Bacillus
UniRef100_P37807

Bacillus subtilis

subtilis]

1705
Hypothetical protein yloU [Bacillus
UniRef100_O34318

Bacillus subtilis

YloU

subtilis]

1706

YloV

1707
Probable L-serine dehydratase, beta
UniRef100_O34635

Bacillus subtilis

SdaAB

chain [Bacillus subtilis]

1708
Probable L-serine dehydratase, alpha
UniRef100_O34607

Bacillus subtilis

SdaAA

chain [Bacillus subtilis]

1709
ATP-dependent DNA helicase recG
UniRef100_O34942

Bacillus subtilis

RecG

[Bacillus subtilis]

1710
Transcription factor fapR [Bacillus
UniRef100_O34835

Bacillus subtilis

YlpC

subtilis]

1711
Fatty acid/phospholipid synthesis
UniRef100_P71018

Bacillus subtilis

PlsX

protein plsX [Bacillus subtilis]

1712
Malonyl CoA-acyl carrier protein
UniRef100_P71019

Bacillus subtilis

FabD

transacylase [Bacillus subtilis]

1713
3-oxoacyl-[acyl-carrier-protein]
UniRef100_P51831
acyl-carrier-
FabG

reductase [Bacillus subtilis]

protein

1714
Acyl carrier protein [Bacillus subtilis]
UniRef100_P80643

Bacillus subtilis

1715

Rnc

1716
Chromosome partition protein smc
UniRef100_P51834

Bacillus subtilis

Smc

[Bacillus subtilis]

1717

FtsY

1718

1719
Signal recognition particle protein
UniRef100_P37105

Bacillus subtilis

Ffh

[Bacillus subtilis]

1720
30S ribosomal protein S16 [Bacillus
UniRef100_P21474

Bacillus subtilis

subtilis]

1721

1722
YlqD protein [Bacillus subtilis]
UniRef100_O31739

Bacillus subtilis

YlqD

1723

RimM

1724

TrmD

1725
50S ribosomal protein L19 [Bacillus
UniRef100_O31742

Bacillus subtilis

RplS

subtilis]

1726
YlqF protein [Bacillus subtilis]
UniRef100_O31743

Bacillus subtilis

YlqF

1727
Ribonuclease HII [Bacillus subtilis]
UniRef100_O31744

Bacillus subtilis

RnhB

1728
YlqG protein [Bacillus subtilis]
UniRef100_O31745

Bacillus subtilis

YlqG

1729
YlqH protein [Bacillus subtilis]
UniRef100_O34867

Bacillus subtilis

1730
Succinyl-CoA synthetase beta chain
UniRef100_P80886

Bacillus subtilis

SucC

[Bacillus subtilis]

1731
Succinyl-CoA synthetase alpha chain
UniRef100_P80865

Bacillus subtilis

SucD

[Bacillus subtilis]

1732

Smf

1733

TopA

1734
Protein gid [Bacillus subtilis]
UniRef100_P39815

Bacillus subtilis

Gid

1735
Tyrosine recombinase xerC [Bacillus
UniRef100_P39776

Bacillus subtilis

CodV

subtilis]

1736
ATP-dependent protease hslV
UniRef100_P39070

Bacillus subtilis

ClpQ

precursor [Bacillus subtilis]

1737
ATP-dependent hsl protease ATP-
UniRef100_P39778

Bacillus subtilis

ClpY

binding subunit hslU [Bacillus subtilis]

1738
GTP-sensing transcriptional pleiotropic
UniRef100_P39779

Bacillus subtilis

CodY

repressor codY [Bacillus subtilis]

1739
Flagellar basal-body rod protein flgB
UniRef100_P24500

Bacillus subtilis

FlgB

[Bacillus subtilis]

1740
Flagellar basal-body rod protein flgC
UniRef100_P24501

Bacillus subtilis

FlgC

[Bacillus subtilis]

1741
Flagellar hook-basal body complex
UniRef100_P24502

Bacillus subtilis

FliE

protein fliE [Bacillus subtilis]

1742
Flagellar M-ring protein [Bacillus
UniRef100_P23447

Bacillus subtilis

FliF

subtilis]

1743
Flagellar motor switch protein fliG
UniRef100_P23448

Bacillus subtilis

FliG

[Bacillus subtilis]

1744
Probable flagellar assembly protein fliH
UniRef100_P23449

Bacillus subtilis

FliH

[Bacillus subtilis]

1745
Flagellum-specific ATP synthase
UniRef100_P23445

Bacillus subtilis

FliI

[Bacillus subtilis]

1746
Flagellar fliJ protein [Bacillus subtilis]
UniRef100_P20487

Bacillus subtilis

FliJ

1747
FlaA locus 22.9 kDa protein [Bacillus
UniRef100_P23454

Bacillus subtilis

YlxF

subtilis]

1748
Probable flagellar hook-length control
UniRef100_P23451

Bacillus subtilis

FliK

protein [Bacillus subtilis]

1749
FlaA locus hypothetical protein ylxG
UniRef100_P23455

Bacillus subtilis

YlxG

[Bacillus subtilis]

1750

FlgE

1751
BH2448 protein [Bacillus halodurans]
UniRef100_Q9KA42

Bacillus halodurans

1752

Flagellar fliL protein [Bacillus subtilis]
UniRef100_P23452

Bacillus subtilis

FliL

1753

Flagellar motor switch protein fliM
UniRef100_P23453

Bacillus subtilis

FliM

[Bacillus subtilis]

1754

Flagellar motor switch protein fliY
UniRef100_P24073

Bacillus subtilis

FliY

[Bacillus subtilis]

1755
Chemotaxis protein cheY homolog
UniRef100_P24072

Bacillus subtilis

CheY

[Bacillus subtilis]

1756

Flagellar biosynthetic protein fliZ
UniRef100_P35536

Bacillus subtilis

FliZ

precursor [Bacillus subtilis]

1757

Flagellar biosynthetic protein fliP
UniRef100_P35528

Bacillus subtilis

FliP

[Bacillus subtilis]

1758

Flagellar biosynthetic protein fliQ
UniRef100_P35535

Bacillus subtilis

[Bacillus subtilis]

1759

Flagellar biosynthetic protein fliR
UniRef100_P35537

Bacillus subtilis

FliR

[Bacillus subtilis]

1760

Flagellar biosynthetic protein flhB
UniRef100_P35538

Bacillus subtilis

FlhB

[Bacillus subtilis]

1761

Flagellar biosynthesis protein flhA
UniRef100_P35620

Bacillus subtilis

FlhA

[Bacillus subtilis]

1762

Flagellar biosynthesis protein flhF
UniRef100_Q01960

Bacillus subtilis

FlhF

[Bacillus subtilis]

1763
Hypothetical protein ylxH [Bacillus
UniRef100_P40742

Bacillus subtilis

YlxH

subtilis]

1764
Chemotaxis response regulator protein-
UniRef100_Q05522

Bacillus subtilis

CheB

glutamate methylesterase [Bacillus

subtilis]

1765
Chemotaxis protein cheA [Bacillus
UniRef100_P29072

Bacillus subtilis

CheA

subtilis]

1766
Chemotaxis protein cheW [Bacillus
UniRef100_P39802

Bacillus subtilis

CheW

subtilis]

1767
Chemotaxis protein cheC [Bacillus
UniRef100_P40403

Bacillus subtilis

CheC

subtilis]

1768
Chemotaxis protein cheD [Bacillus
UniRef100_P40404

Bacillus subtilis

CheD

subtilis]

1769
RNA polymerase sigma-D factor
UniRef100_P10726

Bacillus subtilis

SigD

[Bacillus subtilis]

1770
Swarming motility protein swrB [Bacillus
UniRef100_P40405

Bacillus subtilis

YlxL

subtilis]

1771
30S ribosomal protein S2 [Bacillus
UniRef100_P21464

Bacillus subtilis

RpsB

subtilis]

1772
Translation elongation factor Ts
UniRef100_Q65JJ8

Bacillus cereus

Tsf

[Bacillus cereus]

1773

PyrH

1774

Frr

1775
Undecaprenyl pyrophosphate
UniRef100_O31751

Bacillus subtilis

UppS

synthetase [Bacillus subtilis]

1776
Phosphatidate cytidylyltransferase
UniRef100_O31752

Bacillus subtilis

CdsA

[Bacillus subtilis]

1777
1-deoxy-D-xylulose 5-phosphate
UniRef100_O31753

Bacillus subtilis

Dxr

reductoisomerase [Bacillus subtilis]

1778
Hypothetical zinc metalloprotease yluc
UniRef100_O31754

Bacillus subtilis

YluC

[Bacillus subtilis]

1779
Prolyl-tRNA synthetase [Bacillus
UniRef100_O31755

Bacillus subtilis

ProS

subtilis]

1780
DNA polymerase III polC-type [Bacillus
UniRef100_P13267

Bacillus subtilis

PolC

subtilis]

1781

1782
Cellulose 1,4-beta-cellobiosidase
UniRef100_Q8KKF7

Paenibacillus sp. BP-23

precursor [Paenibacillus sp. BP-23]

1783
Endoglucanase B precursor
UniRef100_P23550

Paenibacillus lautus

[Paenibacillus lautus]

1784
Beta-mannosidase [Thermotoga
UniRef100_Q9RIK7

Thermotoga neapolitana

neapolitana]

1785
Hypothetical UPF0090 protein ylxS
UniRef100_P32726

Bacillus subtilis

YlxS

[Bacillus subtilis]

1786
Transcription elongation protein nusA
UniRef100_P32727

Bacillus subtilis

NusA

[Bacillus subtilis]

1787
Hypothetical protein ylxR [Bacillus
UniRef100_P32728

Bacillus subtilis

subtilis]

1788
Probable ribosomal protein ylxQ
UniRef100_P32729

Bacillus subtilis

[Bacillus subtilis]

1789
Translation initiation factor IF-2
UniRef100_P17889

Bacillus subtilis

InfB

[Bacillus subtilis]

1790
Hypothetical protein ylxP [Bacillus
UniRef100_P32730

Bacillus subtilis

subtilis]

1791
Ribosome-binding factor A [Bacillus
UniRef100_P32731

Bacillus subtilis

RbfA

subtilis]

1792

TruB

1793
Riboflavin biosynthesis protein ribC
UniRef100_P54575
Includes:
RibC

[Includes: Riboflavin kinase (EC

Riboflavin kinase

2.7.1.26) (Flavokinase); FMN

(EC 2.7.1.26)

adenylyltransferase (EC 2.7.7.2) (FAD

(Flavokinase);

pyrophosphorylase) (FAD synthetase)]

FMN

[Bacillus subtilis]

adenylyltransferase

(EC 2.7.7.2)

(FAD

pyrophosphorylase)

(FAD

synthetase)

1794
30S ribosomal protein S15 [Bacillus
UniRef100_P21473

Bacillus subtilis

subtilis]

1795
Polyribonucleotide
UniRef100_P50849

Bacillus subtilis

PnpA

nucleotidyltransferase [Bacillus subtilis]

1796
Hypothetical protein ylxY precursor
UniRef100_P50850

Bacillus subtilis

YlxY

[Bacillus subtilis]

1797
Hypothetical zinc protease ymxG
UniRef100_Q04805

Bacillus subtilis

MlpA

[Bacillus subtilis]

1798
Hypothetical protein ymxH [Bacillus
UniRef100_Q04811

Bacillus subtilis

subtilis]

1799
Dipicolinate synthase, A chain [Bacillus
UniRef100_Q04809

Bacillus subtilis

SpoVFA

subtilis]

1800
Dipicolinate synthase, B chain [Bacillus
UniRef100_Q04810

Bacillus subtilis

SpoVFB

subtilis]

1801
Aspartate-semialdehyde
UniRef100_Q04797

Bacillus subtilis

Asd

dehydrogenase [Bacillus subtilis]

1802
Aspartokinase 1 (EC 2.7.2.4)
UniRef100_Q04795
Contains:
DapG

(Aspartokinase I) (Aspartate kinase 1)

Aspartokinase I

[Contains: Aspartokinase I alpha

alpha subunit;

subunit; Aspartokinase I beta subunit]

Aspartokinase I

[Bacillus subtilis]

beta subunit

1803
Dihydrodipicolinate synthase [Bacillus
UniRef100_Q04796

Bacillus subtilis

DapA

subtilis]

1804
YmfA protein [Bacillus subtilis]
UniRef100_O31760

Bacillus subtilis

YmfA

1805
Translocation-enhancing protein tepA
UniRef100_Q99171

Bacillus subtilis

TepA

[Bacillus subtilis]

1806

1807
DNA translocase ftsK [Bacillus subtilis]
UniRef100_P21458

Bacillus subtilis

SpoIIIE

1808
Hypothetical transcriptional regulator
UniRef100_O31761

Bacillus subtilis

YmfC

ymfC [Bacillus subtilis]

1809
Multidrug resistance protein [Bacillus
UniRef100_Q9K7Q2

Bacillus

YitG

halodurans]

halodurans

1810
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81WP6

Bacillus

YmfG

anthracis

1811
YmfH protein [Bacillus subtilis]
UniRef100_O31766

Bacillus subtilis

YmfH

1812

1813

1814
YmfJ protein [Bacillus subtilis]
UniRef100_O31768

Bacillus subtilis

1815
Hypothetical protein [Bacillus cereus
UniRef100_Q636P2

Bacillus cereus

YmfK

ZK]

ZK

1816

YmfM

1817
CDP-diacylglycerol--glycerol-3-
UniRef100_P46322

Bacillus subtilis

PgsA

phosphate 3-phosphatidyltransferase

[Bacillus subtilis]

1818
CinA-like protein [Bacillus subtilis]
UniRef100_P46323

Bacillus subtilis

CinA

1819
RecA protein [Bacillus
UniRef100_Q8GJG2

Bacillus

RecA

amyloliquefaciens]

amyloliquefaciens

1820
Hypothetical UPF0144 protein ymdA
UniRef100_O31774

Bacillus subtilis

YmdA

[Bacillus subtilis]

1821
YmdB protein [Bacillus subtilis]
UniRef100_O31775

Bacillus subtilis

YmdB

1822
Stage V sporulation protein S [Bacillus
UniRef100_P45693

Bacillus subtilis

subtilis]

1823

1824

1825
L-threonine 3-dehydrogenase [Bacillus
UniRef100_O31776

Bacillus subtilis

Tdh

subtilis]

1826
2-amino-3-ketobutyrate coenzyme A
UniRef100_O31777

Bacillus subtilis

Kbl

ligase [Bacillus subtilis]

1827
Hypothetical UPF0004 protein ymcB
UniRef100_O31778

Bacillus subtilis

YmcB

[Bacillus subtilis]

1828
YmcA protein [Bacillus subtilis]
UniRef100_O31779

Bacillus subtilis

YmcA

1829
Spore coat protein E [Bacillus subtilis]
UniRef100_P14016

Bacillus subtilis

CotE

1830

MutS

1831
DNA mismatch repair protein mutL
UniRef100_P49850

Bacillus subtilis

MutL

[Bacillus subtilis]

1832
YjcS protein [Bacillus subtilis]
UniRef100_O31641

Bacillus subtilis

1833

YxiD

1834

1835

1836
All1751 protein [Anabaena sp.]
UniRef100_Q8YW65

Anabaena sp.
YciC

1837
BH0367 protein [Bacillus halodurans]
UniRef100_Q9KFV4

Bacillus halodurans

1838
Phosphinothricin N-acetyltransferase
UniRef100_Q9KFP5

Bacillus

YwnH

[Bacillus halodurans]

halodurans

1839
UPI00003CC0D8 UniRef100 entry
UniRef100_UPI00003CC0D8

1840

1841
Putative L-amino acid oxidase [Bacillus
UniRef100_O34363

Bacillus subtilis

YobN

subtilis]

1842

YoaK

1843
Na+/H+ antiporter [Bacillus halodurans]
UniRef100_Q9K5Q0

Bacillus

YvgP

halodurans

1844
Aromatic hydrocarbon catabolism
UniRef100_Q8CV32

Oceanobacillus iheyensis

protein [Oceanobacillus iheyensis]

1845
Hypothetical UPF0145 protein VP1283
UniRef100_Q87Q67

Vibrio parahaemolyticus

[Vibrio parahaemolyticus]

1846
Hypothetical protein yqeD [Bacillus
UniRef100_P54449

Bacillus subtilis

YqeD

subtilis]

1847
Penicillin-binding protein, putative
UniRef100_Q738U9

Bacillus cereus

PbpE

[Bacillus cereus]

1848
Hypothetical glycosyl transferase
UniRef100_O34539

Bacillus subtilis

YjiC

[Bacillus subtilis]

1849
Asparate-proton symporter [Bacillus
UniRef100_O07002

Bacillus subtilis

YveA

subtilis]

1850
Spore coat protein [Bacillus halodurans]
UniRef100_Q9KEV6

Bacillus halodurans

1851

1852

YdhD

1853
Putative HTH-type transcriptional
UniRef100_Q7WY76

Bacillus subtilis

YezE

regulator yezE [Bacillus subtilis]

1854
Hypothetical protein yesE [Bacillus
UniRef100_O31511

Bacillus subtilis

YesE

subtilis]

1855
YesF protein [Bacillus subtilis]
UniRef100_O31512

Bacillus subtilis

YesF

1856
UPI00003CBA3B UniRef100 entry
UniRef100_UPI00003CBA3B

1857
YmaD protein [Bacillus subtilis]
UniRef100_O31790

Bacillus subtilis

YmaD

1858
Multidrug resistance protein ebrB
UniRef100_O31791

Bacillus subtilis

EbrB

[Bacillus subtilis]

1859
Multidrug resistance protein ebrA
UniRef100_O31792

Bacillus subtilis

EbrA

[Bacillus subtilis]

1860

1861
Hypothetical protein ymaF [Bacillus
UniRef100_O31794

Bacillus subtilis

YmaF

subtilis]

1862
tRNA delta(2)-
UniRef100_O31795

Bacillus subtilis

MiaA

isopentenylpyrophosphate transferase

[Bacillus subtilis]

1863
Hfq protein [Bacillus subtilis]
UniRef100_O31796

Bacillus subtilis

1864
Hypothetical protein ymzA [Bacillus
UniRef100_O31798

Bacillus subtilis

subtilis]

1865
NrdI protein [Bacillus subtilis]
UniRef100_P50618

Bacillus subtilis

YmaA

1866
Ribonucleoside-diphosphate reductase
UniRef100_P50620

Bacillus subtilis

NrdE

alpha chain [Bacillus subtilis]

1867
Ribonucleoside-diphosphate reductase
UniRef100_P50621

Bacillus subtilis

NrdF

beta chain [Bacillus subtilis]

1868
Hypothetical protein ymaB [Bacillus
UniRef100_P50619

Bacillus subtilis

YmaB

subtilis]

1869
Blr6966 protein [Bradyrhizobium
UniRef100_Q89EV4

Bradyrhizobium

YtnP

japonicum]

japonicum

1870
Nitrogen fixation protein [Bacillus
UniRef100_Q9KFV2

Bacillus

YurV

halodurans]

halodurans

1871
Transcription regulator Fur family-like
UniRef100_Q8CNQ7

Staphylococcus

PerR

protein [Staphylococcus epidermidis]

epidermidis

1872
UPI00003CB681 UniRef100 entry
UniRef100_UPI00003CB681

YdhC

1873
Hypothetical protein [Bacillus cereus]
UniRef100_Q81C60

Bacillus cereus

YjlA

1874

CwlC

1875
Membrane protein, putative [Listeria
UniRef100_Q720L9

Listeria monocytogenes

monocytogenes]

1876
Lin1174 protein [Listeria innocua]
UniRef100_Q92CJ7

Listeria innocua

1877

1878

1879
Transcriptional regulator [Aquifex
UniRef100_O66635

Aquifex aeolicus

YdgC

aeolicus]

1880
Hypothetical membrane-anchored
UniRef100_Q92VA1

Rhizobium meliloti

protein [Rhizobium meliloti]

1881
Cytosine permease [Bacillus
UniRef100_Q9KBP3

Bacillus

YxlA

halodurans]

halodurans

1882
AgaF [Agrobacterium tumefaciens]
UniRef100_O50265

Agrobacterium tumefaciens

1883

1884
Hydantoin utilization protein B
UniRef100_Q88H51

Pseudomonas putida

[Pseudomonas putida]

1885
Hypothetical protein SMb20139
UniRef100_Q92X23

Rhizobium meliloti

[Rhizobium meliloti]

1886
BH2340 protein [Bacillus halodurans]
UniRef100_Q9KAE7

Bacillus halodurans

1887

1888

1889
Stage V sporulation protein K [Bacillus
UniRef100_P27643

Bacillus subtilis

SpoVK

subtilis]

1890
YnbA [Bacillus subtilis]
UniRef100_P94478

Bacillus subtilis

YnbA

1891
YnbB [Bacillus subtilis]
UniRef100_P94479

Bacillus subtilis

YnbB

1892
HTH-type transcriptional regulator glnR
UniRef100_P37582

Bacillus subtilis

GlnR

[Bacillus subtilis]

1893
Glutamine synthetase [Bacillus subtilis]
UniRef100_P12425

Bacillus subtilis

GlnA

1894

1895
Hypothetical protein CAC3435
UniRef100_Q97DN7

Clostridium acetobutylicum

[Clostridium acetobutylicum]

1896

1897

1898
Hypothetical protein CAC0350
UniRef100_Q97M50

Clostridium acetobutylicum

[Clostridium acetobutylicum]

1899
Hypothetical HIT-like protein MJ0866
UniRef100_Q58276

Methanococcus

Hit

[Methanococcus jannaschii]

jannaschii

1900
Hypothetical protein [Bacillus anthracis]
UniRef100_Q6I2B3

Bacillus anthracis

1901
Hypothetical protein [Bacillus cereus]
UniRef100_Q81CM1

Bacillus cereus

YdjC

1902

1903
Methyltransferase [Bacillus cereus]
UniRef100_Q81CJ2

Bacillus cereus

1904

YoaO

1905
Acetyltransferase, GNAT family
UniRef100_Q737B4

Bacillus cereus

[Bacillus cereus]

1906
Repressor rok [Bacillus subtilis]
UniRef100_O34857

Bacillus subtilis

Rok

1907

1908
Hypothetical protein [Bacteriophage T5]
UniRef100_Q6QGK2
Bacteriophage T5

1909
Hypothetical protein yvdT [Bacillus
UniRef100_O07001

Bacillus subtilis

YvdT

subtilis]

1910
Hypothetical protein yvdS [Bacillus
UniRef100_O32262

Bacillus subtilis

YvdS

subtilis]

1911
Hypothetical protein yvdR [Bacillus
UniRef100_O06999

Bacillus subtilis

YvdR

subtilis]

1912
Spermidine N1-acetyltransferase
UniRef100_Q72Y03

Bacillus cereus

YoaA

[Bacillus cereus]

1913
Hypothetical Membrane Associated
UniRef100_Q812L6

Bacillus cereus

Protein [Bacillus cereus]

1914
Hypothetical protein yoaW precursor
UniRef100_O34541

Bacillus subtilis

YoaW

[Bacillus subtilis]

1915
Thiol-disulfide oxidoreductase resA
UniRef100_Q9KCJ4

Bacillus

ResA

[Bacillus halodurans]

halodurans

1916
Manganese-containing catalase
UniRef100_Q9KAU6

Bacillus

YdbD

[Bacillus halodurans]

halodurans

1917
BH1562 protein [Bacillus halodurans]
UniRef100_Q9KCK9

Bacillus halodurans

1918
Acetyltransferase, GNAT family
UniRef100_Q739K0

Bacillus cereus

YjcK

[Bacillus cereus]

1919
Hypothetical conserved protein
UniRef100_Q8ELR7

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

1920

1921
Transcriptional regulator, MarR family
UniRef100_Q81BM5

Bacillus cereus

YkvE

[Bacillus cereus]

1922
Putative NAD(P)H nitroreductase ydfN
UniRef100_P96692

Bacillus subtilis

YdfN

[Bacillus subtilis]

1923
YdfO protein [Bacillus subtilis]
UniRef100_P96693

Bacillus subtilis

YdfO

1924
Hypothetical protein [Bacillus cereus
UniRef100_Q630S7

Bacillus cereus

YwrF

ZK]

ZK

1925
BH1010 protein [Bacillus halodurans]
UniRef100_Q9KE48

Bacillus

YhcB

halodurans

1926
ORF28 [Staphylococcus phage K]
UniRef100_Q6Y7T8

Staphylococcus phage K

1927

1928
Thymidylate synthase [Bacteriophage
UniRef100_P07606
Bacteriophage
ThyA

phi-3T]

phi-3T

1929
Sporulation-specific extracellular
UniRef100_P42983

Bacillus subtilis

NucB

nuclease precursor [Bacillus subtilis]

1930
LexA repressor [Bacillus subtilis]
UniRef100_P31080

Bacillus subtilis

LexA

1931
YneA [Bacillus subtilis]
UniRef100_Q45056

Bacillus subtilis

YneA

1932
YneB [Bacillus subtilis]
UniRef100_Q45057

Bacillus subtilis

YneB

1933
Hypothetical UPF0291 protein ynzC
UniRef100_O31818

Bacillus subtilis

[Bacillus subtilis]

1934
Transketolase [Bacillus subtilis]
UniRef100_P45694

Bacillus subtilis

Tkt

1935
Hypothetical protein yneE [Bacillus
UniRef100_P45707

Bacillus subtilis

YneE

subtilis]

1936
Hypothetical UPF0154 protein yneF
UniRef100_P45708

Bacillus subtilis

[Bacillus subtilis]

1937

1938
Cytochrome c-type biogenesis protein
UniRef100_P45706

Bacillus subtilis

CcdA

ccdA [Bacillus subtilis]

1939
CcdB protein [Bacillus subtilis]
UniRef100_P45709

Bacillus subtilis

YneI

1940
CcdC protein [Bacillus subtilis]
UniRef100_P45710

Bacillus subtilis

YneJ

1941
Hypothetical protein yneK [Bacillus
UniRef100_P45711

Bacillus subtilis

YneK

subtilis]

1942
Spore coat protein M [Bacillus subtilis]
UniRef100_Q45058

Bacillus subtilis

CotM

1943

1944

1945

CitB

1946
YneN protein [Bacillus subtilis]
UniRef100_O31820

Bacillus subtilis

YneN

1947

1948

1949
Small, acid-soluble spore protein tlp
UniRef100_Q45060

Bacillus subtilis

[Bacillus subtilis]

1950
YneP [Bacillus subtilis]
UniRef100_Q45061

Bacillus subtilis

YneP

1951
YneQ [Bacillus subtilis]
UniRef100_Q45062

Bacillus subtilis

1952
Hypothetical protein [Bacillus cereus]
UniRef100_Q815P1

Bacillus cereus

1953
Conserved domain protein [Bacillus
UniRef100_Q72YR7

Bacillus cereus

cereus]

1954
YneR [Bacillus subtilis]
UniRef100_Q45063

Bacillus subtilis

1955
Hypothetical UPF0078 protein yneS
UniRef100_Q45064

Bacillus subtilis

YneS

[Bacillus subtilis]

1956
YneT [Bacillus subtilis]
UniRef100_Q45065

Bacillus subtilis

YneT

1957
Topoisomerase IV subunit B [Bacillus
UniRef100_Q59192

Bacillus subtilis

ParE

subtilis]

1958
Topoisomerase IV subunit A [Bacillus
UniRef100_Q45066

Bacillus subtilis

ParC

subtilis]

1959

AraR

1960
Hypothetical conserved protein
UniRef100_Q8EMP2

Oceanobacillus

XylB

[Oceanobacillus iheyensis]

iheyensis

1961
L-ribulose-5-phosphate 4-epimerase
UniRef100_Q8EMP3

Oceanobacillus

AraD

[Oceanobacillus iheyensis]

iheyensis

1962
L-arabinose isomerase [Oceanobacillus
UniRef100_Q8EMP4

Oceanobacillus

AraA

iheyensis]

iheyensis

1963

YwtG

1964

FabG

1965
Hypothetical protein ynfC [Bacillus
UniRef100_Q45067

Bacillus subtilis

YnfC

subtilis]

1966
Amino acid carrier protein alsT [Bacillus
UniRef100_Q45068

Bacillus subtilis

AlsT

subtilis]

1967

NarI

1968

NarJ

1969

NarH

1970

NarG

1971

1972
Hypothetical protein yqfB [Lactococcus
UniRef100_Q9CF70

Lactococcus lactis

lactis]

1973

AlbA

1974

ArfM

1975

YwiC

1976
Transcriptional regulator of anaerobic
UniRef100_Q9KG81

Bacillus

Fnr

genes [Bacillus halodurans]

halodurans

1977
Nitrite extrusion protein [Bacillus
UniRef100_P46907

Bacillus subtilis

NarK

subtilis]

1978

1979
CAMP-binding domains-Catabolite
UniRef100_Q8R5P4

Thermoanaerobacter

Fnr

gene activator and regulatory subunit of

tengcongensis

cAMP-dependent protein kinases

[Thermoanaerobacter tengcongensis]

1980
Putative nitric oxide reductase
UniRef100_Q6GK48

Staphylococcus aureus

[Staphylococcus aureus]

1981

1982

YngL

1983

BglC

1984
Hypothetical protein ynfE [Bacillus
UniRef100_Q45069

Bacillus subtilis

subtilis]

1985
Hypothetical protein [Bacillus
UniRef100_Q9ZF48

Bacillus

YkkB

megaterium]

megaterium

1986
Hypothetical protein [Bacillus
UniRef100_Q70K06

Bacillus amyloliquefaciens

amyloliquefaciens]

1987
Alkyl hydroperoxide reductase large
UniRef100_Q9Z9W3

Bacillus

AhpF

subunit [Bacillus halodurans]

halodurans

1988
Methyltransferase [Methanosarcina
UniRef100_Q8PU82

Methanosarcina mazei

mazei]

1989
Similar to B. subtilis ywgB gene [Bacillus
UniRef100_Q9Z9W2

Bacillus

YwgB

halodurans]

halodurans

1990
Hypothetical protein ywoF [Bacillus
UniRef100_P94576

Bacillus subtilis

YwoF

subtilis]

1991
Branched-chain amino acid transport
UniRef100_P94499

Bacillus subtilis

BrnQ

system carrier protein brnQ [Bacillus

subtilis]

1992
NADP-dependent alcohol
UniRef100_O06007

Bacillus subtilis

AdhA

dehydrogenase [Bacillus subtilis]

1993
Transcriptional regulator, MerR family
UniRef100_Q721Z3

Listeria

YraB

[Listeria monocytogenes]

monocytogenes

1994
HPr-like protein crh [Bacillus subtilis]
UniRef100_O06976

Bacillus subtilis

1995
BH2089 protein [Bacillus halodurans]
UniRef100_Q9Z9R4

Bacillus

YddR

halodurans

1996

1997
Enoyl-CoA hydratase/isomerase family
UniRef100_Q738L0

Bacillus cereus

YngF

protein [Bacillus cereus]

1998
Hypothetical protein ysiB [Bacillus
UniRef100_P94549

Bacillus subtilis

YsiB

subtilis]

1999
Methylmalonic acid semialdehyde
UniRef100_Q63BL0

Bacillus cereus

MmsA

dehydrogenase [Bacillus cereus ZK]

ZK

2000
3-hydroxyisobutyrate dehydrogenase
UniRef100_Q63BL1

Bacillus cereus

YkwC

[Bacillus cereus ZK]

ZK

2001
Acyl-CoA dehydrogenase [Bacillus
UniRef100_Q81DR7

Bacillus cereus

YusJ

cereus]

2002
Mannose-6-phosphate isomerase
UniRef100_O31646

Bacillus subtilis

ManA

[Bacillus subtilis]

2003
Phosphotransferase system (PTS)
UniRef100_O31645

Bacillus subtilis

ManP

mannose-specific enzyme IIBCA

component [Bacillus subtilis]

2004

2005

2006
Hypothetical protein [Bacillus cereus]
UniRef100_Q72YT6

Bacillus cereus

2007
Transcriptional regulator [Bacillus
UniRef100_O31644

Bacillus subtilis

ManR

subtilis]

2008

2009
UPI00003CC220 UniRef100 entry
UniRef100_UPI00003CC220

YtrB

2010
Transcriptional regulator [Bacillus
UniRef100_Q9KF35

Bacillus

YtrA

halodurans]

halodurans

2011
Probable oxidoreductase [Clostridium
UniRef100_Q8XP17

Clostridium

YjmF

perfringens]

perfringens

2012
Mannonate dehydratase [Clostridium
UniRef100_Q8XP15

Clostridium

UxuA

perfringens]

perfringens

2013
Glucosidase [Bacillus halodurans]
UniRef100_Q9KEZ5

Bacillus halodurans

2014
C4-dicarboxylate transport system
UniRef100_Q9KEZ6

Bacillus halodurans

[Bacillus halodurans]

2015
C4-dicarboxylate transport system
UniRef100_Q8EMM5

Oceanobacillus iheyensis

permease small protein

[Oceanobacillus iheyensis]

2016
C4-dicarboxylate transport system
UniRef100_Q9KEZ8

Bacillus

DctB

[Bacillus halodurans]

halodurans

2017
Transcriptional regulator
UniRef100_Q8EL22

Oceanobacillus

CcpA

[Oceanobacillus iheyensis]

iheyensis

2018
Arsenate reductase [Bacillus subtilis]
UniRef100_P45947

Bacillus subtilis

ArsC

2019
YdfA protein [Bacillus subtilis]
UniRef100_P96678

Bacillus subtilis

YdfA

2020
YdeT protein [Bacillus subtilis]
UniRef100_P96677

Bacillus subtilis

YdeT

2021
YdeI [Bacillus halodurans]
UniRef100_Q9Z9R5

Bacillus

YdeI

halodurans

2022
Putative secreted protein
UniRef100_Q9S1Z5

Streptomyces coelicolor

[Streptomyces coelicolor]

2023
Probable glucose uptake protein glcU
UniRef100_P40419

Bacillus

GlcU

[Bacillus megaterium]

megaterium

2024
YngK protein [Bacillus subtilis]
UniRef100_O35015

Bacillus subtilis

YngK

2025
YngD protein [Bacillus subtilis]
UniRef100_O31824

Bacillus subtilis

YngD

2026
Pyruvate formate-lyase-activating
UniRef100_Q73DZ6

Bacillus cereus

YkvL

enzyme [Bacillus cereus]

2027
Formate acetyltransferase [Bacillus
UniRef100_Q81YX1

Bacillus anthracis

anthracis]

2028

DacC

2029
NADH dehydrogenase-like protein yjlD
UniRef100_P80861

Bacillus subtilis

YjlD

[Bacillus subtilis]

2030
Hypothetical protein yjlC [Bacillus
UniRef100_O34633

Bacillus subtilis

YjlC

subtilis]

2031

2032
Hypothetical protein [Bacillus cereus]
UniRef100_Q81IJ8

Bacillus cereus

2033
Hypothetical protein ykzH [Bacillus
UniRef100_O31653

Bacillus subtilis

subtilis]

2034
Acetyl-CoA synthetase [Bacillus
UniRef100_Q9KDS4

Bacillus

AcsA

halodurans]

halodurans

2035
YngE protein [Bacillus subtilis]
UniRef100_O31825

Bacillus subtilis

YngE

2036
Hydroxybutyryl-dehydratase [Bacillus
UniRef100_Q9L7W1

Bacillus subtilis

YngF

subtilis]

2037
YngG protein [Bacillus subtilis]
UniRef100_O34873

Bacillus subtilis

YngG

2038
YngXX [Bacillus subtilis]
UniRef100_Q9R9I3

Bacillus subtilis

2039
YngH [Bacillus subtilis]
UniRef100_Q9R9I4

Bacillus subtilis

YngH

2040
YngI [Bacillus subtilis]
UniRef100_Q9R9I5

Bacillus subtilis

YngI

2041
YngJ protein [Bacillus subtilis]
UniRef100_O34421

Bacillus subtilis

YngJ

2042
NAD(P)H oxidoreductase YRKL
UniRef100_Q7P6P0

Fusobacterium

YrkL

[Fusobacterium nucleatum subsp.

nucleatum

vincentii ATCC 49256]

subsp. vincentii

ATCC 49256

2043
Transcriptional regulator, MarR family
UniRef100_Q8RE85

Fusobacterium nucleatum

[Fusobacterium nucleatum]

2044
Glutamate-5-semialdehyde
UniRef100_Q6HHC2

Bacillus

ProA

dehydrogenase [Bacillus thuringiensis]

thuringiensis

2045
Glutamate 5-kinase 2 [Bacillus subtilis]
UniRef100_O07509

Bacillus subtilis

ProJ

2046
Pyrroline-5-carboxylate reductase 1
UniRef100_P14383

Bacillus subtilis

ProH

[Bacillus subtilis]

2047
UPI00003CB6CD UniRef100 entry
UniRef100_UPI00003CB6CD

YetF

2048
Sodium-dependent phosphate
UniRef100_Q9KCT1

Bacillus

CysP

transporter [Bacillus halodurans]

halodurans

2049
Probable phosphoadenosine
UniRef100_O06737

Bacillus subtilis

YitB

phosphosulfate reductase [Bacillus

subtilis]

2050
Phosphosulfolactate synthase (EC
UniRef100_O06739

Bacillus subtilis

YitD

4.4.1.19) ((2R)-phospho-3-sulfolactate

synthase) [Bacillus subtilis]

2051
YitE [Bacillus subtilis]
UniRef100_O06740

Bacillus subtilis

YitE

2052
YitF [Bacillus subtilis]
UniRef100_O06741

Bacillus subtilis

YitF

2053
YitG [Bacillus subtilis]
UniRef100_O06742

Bacillus subtilis

YitG

2054
Putative glycosyl transferase ykoT
UniRef100_O34755

Bacillus subtilis

YkoT

[Bacillus subtilis]

2055
YkoR [Bacillus subtilis]
UniRef100_O34830

Bacillus subtilis

YkoS

2056
Glutamate synthase [NADPH] small
UniRef100_O34399
NADPH
GltB

chain [Bacillus subtilis]

2057

GltA

2058
HTH-type transcriptional regulator gltC
UniRef100_P20668

Bacillus subtilis

GltC

[Bacillus subtilis]

2059
AI17121 protein [Anabaena sp.]
UniRef100_Q8YL17

Anabaena sp.

2060

2061
Lmo0606 protein [Listeria
UniRef100_Q8Y9C6

Listeria monocytogenes

monocytogenes]

2062
ABC transporter ATP-binding protein
UniRef100_Q67MU2

Symbiobacterium

YfiB

[Symbiobacterium thermophilum]

thermophilum

2063
ABC transporter ATP-binding protein
UniRef100_Q67MU3

Symbiobacterium

YfiC

[Symbiobacterium thermophilum]

thermophilum

2064
Cytochrome P450 109 [Bacillus subtilis]
UniRef100_P27632

Bacillus subtilis

YjiB

2065
Hypothetical oxidoreductase yoxD
UniRef100_P14802

Bacillus subtilis

YoxD

[Bacillus subtilis]

2066

Pps

2067
Permease, general substrate
UniRef100_Q6HMC3

Bacillus

LmrB

transporter [Bacillus thuringiensis]

thuringiensis

2068
Putative formate dehydrogenase
UniRef100_O34323

Bacillus subtilis

YoaE

[Bacillus subtilis]

2069
Transcriptional regulatory protein
UniRef100_Q89KD1

Bradyrhizobium

YcgE

[Bradyrhizobium japonicum]

japonicum

2070
Drug resistance transporter,
UniRef100_Q73615

Bacillus cereus

Mdr

EmrB/QacA family [Bacillus cereus]

2071
YndE protein [Bacillus subtilis]
UniRef100_O31809

Bacillus subtilis

YndE

2072
YndF protein [Bacillus subtilis]
UniRef100_O31810

Bacillus subtilis

YndF

2073

YndE

2074

YndE

2075
UPI00003CB22E UniRef100 entry
UniRef100_UPI00003CB22E

YndD

2076

2077
Nucleotide binding protein expZ
UniRef100_P39115

Bacillus subtilis

ExpZ

[Bacillus subtilis]

2078

TlpB

2079
CsaA protein [Bacillus subtilis]
UniRef100_P37584

Bacillus subtilis

CsaA

2080
Alkaline serine protease [Bacillus
UniRef100_O31788

Bacillus subtilis

AprX

subtilis]

2081
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81SD4

Bacillus

YnzE

anthracis

2082
Transcriptional regulator, TetR family
UniRef100_Q63D70

Bacillus cereus

YrhI

[Bacillus cereus ZK]

ZK

2083
Putative HTH-type transcriptional
UniRef100_P40762

Bacillus subtilis

YvmB

regulator yvmB [Bacillus subtilis]

2084
Hydrolase [Bacillus cereus]
UniRef100_Q81D79

Bacillus cereus

YqeK

2085
Hypothetical conserved protein
UniRef100_Q8ETG3

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

2086
YndJ protein [Bacillus subtilis]
UniRef100_O31813

Bacillus subtilis

YndJ

2087
YndH protein [Bacillus subtilis]
UniRef100_O31812

Bacillus subtilis

YndH

2088
Hypothetical protein [Bacillus cereus]
UniRef100_Q73A97

Bacillus cereus

YndG

2089
UPI00003CBA97 UniRef100 entry
UniRef100_UPI00003CBA97

YobS

2090
UPI00003CBA98 UniRef100 entry
UniRef100_UPI00003CBA98

YobT

2091
DNA-binding protein YobU [Bacillus
UniRef100_O34637

Bacillus subtilis

YobU

subtilis]

2092
Hypothetical protein [Bacteroides
UniRef100_Q64RP1

Bacteroides fragilis

fragilis]

2093
Transcription regulator [Bacillus subtilis]
UniRef100_O34920

Bacillus subtilis

YobV

2094

YobW

2095
YozA protein [Bacillus subtilis]
UniRef100_O31844

Bacillus subtilis

2096
Possible metallo-beta-lactamase family
UniRef100_Q638G1

Bacillus cereus

YmaE

protein [Bacillus cereus ZK]

ZK

2097

2098

YfnA

2099

YocA

2100
UPI00003CBE6E UniRef100 entry
UniRef100_UPI00003CBE6E

YveM

2101
Hypothetical protein [Bacillus cereus]
UniRef100_Q817C2

Bacillus cereus

2102

2103
Glycosyl transferase [Bacillus
UniRef100_Q9K7I1

Bacillus

YtcC

halodurans]

halodurans

2104
YozB protein [Bacillus subtilis]
UniRef100_O31845

Bacillus subtilis

YozB

2105
Lmo2079 protein [Listeria
UniRef100_Q8Y5I3

Listeria monocytogenes

monocytogenes]

2106
YocC [Bacillus subtilis]
UniRef100_O35042

Bacillus subtilis

YocC

2107
Na+/myo-inositol cotransporter [Bacillus
UniRef100_Q9KAR5

Bacillus

YcgO

halodurans]

halodurans

2108
YocH [Bacillus subtilis]
UniRef100_O34669

Bacillus subtilis

YocH

2109
RecQ homolog [Bacillus subtilis]
UniRef100_O34748

Bacillus subtilis

YocI

2110
Hypothetical protein yqbC [Bacillus
UniRef100_P45919

Bacillus subtilis

YqbC

subtilis]

2111
Hypothetical protein yjfB [Bacillus
UniRef100_O34438

Bacillus subtilis

subtilis]

2112
Hypothetical protein yyaQ [Bacillus
UniRef100_P37507

Bacillus subtilis

YyaQ

subtilis]

2113
Hypothetical protein OB2103
UniRef100_Q8EPJ8

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

2114

2115
Hypothetical protein yjgD [Bacillus
UniRef100_O34681

Bacillus subtilis

YjgD

subtilis]

2116

YjgC

2117

YjgC

2118
Hypothetical protein OB3361
UniRef100_Q8EL70

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

2119
Hypothetical protein ypfA [Bacillus
UniRef100_P38491

Bacillus subtilis

YpfA

subtilis]

2120

2121

2122

YxiB

2123

2124

2125

2126

YndF

2127

2128
Hypothetical protein [Bacillus cereus]
UniRef100_Q816C2

Bacillus cereus

2129
BH0185 protein [Bacillus halodurans]
UniRef100_Q9KGB9

Bacillus halodurans

2130

2131

YisY

2132
Hypothetical protein yoqH
UniRef100_O64117
Bacteriophage
YoqH

[Bacteriophage SPBc2]

SPBc2

2133

2134
BH0429 protein [Bacillus halodurans]
UniRef100_Q9KFP9

Bacillus

YrhP

halodurans

2135
30S ribosomal protein S14
UniRef100_Q8ETX0

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

2136
UPI00002DEBB5 UniRef100 entry
UniRef100_UPI00002DEBB5

MutT

2137
Pyruvate water dikinase
UniRef100_Q8TN35

Methanosarcina

Pps

[Methanosarcina acetivorans]

acetivorans

2138
Transcriptional regulator
UniRef100_Q8ESJ8

Oceanobacillus

YxbF

[Oceanobacillus iheyensis]

iheyensis

2139

2140
YndM protein [Bacillus subtilis]
UniRef100_O31816

Bacillus subtilis

YndM

2141
Hypothetical protein yisT [Bacillus
UniRef100_O07939

Bacillus subtilis

YisT

subtilis]

2142
Putative acyl carrier protein
UniRef100_O35022

Bacillus subtilis

YocJ

phosphodiesterase 1 [Bacillus subtilis]

2143
General stress protein 16O [Bacillus
UniRef100_P80872

Bacillus subtilis

YocK

subtilis]

2144

2145

2146
Aldehyde dehydrogenase [Bacillus
UniRef100_O34660

Bacillus subtilis

DhaS

subtilis]

2147
YjbB protein [Bacillus subtilis]
UniRef100_O31600

Bacillus subtilis

YjbB

2148
Aminoglycoside N6′-acetyltransferase
UniRef100_Q81AT3

Bacillus cereus

YjcK

[Bacillus cereus]

2149
Blasticidin S deaminase, putative
UniRef100_Q81Y61

Bacillus anthracis

[Bacillus anthracis]

2150

SqhC

2151
Probable superoxide dismutase [Fe]
UniRef100_O35023
Fe
SodF

[Bacillus subtilis]

2152
Stress response protein yvgO precursor
UniRef100_O32211

Bacillus subtilis

YvgO

[Bacillus subtilis]

2153
Putative transporter [Bacillus subtilis]
UniRef100_O34383

Bacillus subtilis

YocR

2154
Putative transporter [Bacillus subtilis]
UniRef100_O34524

Bacillus subtilis

YocS

2155
Dihydrolipoyllysine-residue
UniRef100_P16263

Bacillus subtilis

OdhB

succinyltransferase component of 2-

oxoglutarate dehydrogenase complex

[Bacillus subtilis]

2156
2-oxoglutarate dehydrogenase E1
UniRef100_P23129

Bacillus subtilis

OdhA

component [Bacillus subtilis]

2157
YojO protein [Bacillus subtilis]
UniRef100_O31849

Bacillus subtilis

YojO

2158
YojN protein [Bacillus subtilis]
UniRef100_O31850

Bacillus subtilis

YojN

2159
Hypothetical superoxide dismutase-like
UniRef100_O31851

Bacillus subtilis

YojM

protein yojM precursor [Bacillus subtilis]

2160
Hypothetical protein yojL precursor
UniRef100_O31852

Bacillus subtilis

LytF

[Bacillus subtilis]

2161
Probable multidrug resistance protein
UniRef100_O31855

Bacillus subtilis

YojI

norM (Na(+)/drug antiporter) [Bacillus

subtilis]

2162
Hypothetical protein [Bacillus cereus
UniRef100_Q637Z8

Bacillus cereus

YojG

ZK]

ZK

2163
YojF protein [Bacillus subtilis]
UniRef100_O31858

Bacillus subtilis

YojF

2164
YojE [Bacillus subtilis]
UniRef100_O68260

Bacillus subtilis

2165
YojE protein [Bacillus subtilis]
UniRef100_O31859

Bacillus subtilis

YojE

2166
Hypothetical protein yozR [Bacillus
UniRef100_Q7WY67

Bacillus subtilis

YozR

subtilis]

2167
YoaJ [Bacillus subtilis]
UniRef100_O34918

Bacillus subtilis

YoaJ

2168

2169
UPI00003CBE9B UniRef100 entry
UniRef100_UPI00003CBE9B

YwfD

2170
Hypothetical UPF0087 protein yodB
UniRef100_O34844

Bacillus subtilis

YodB

[Bacillus subtilis]

2171
Putative NAD(P)H nitroreductase 12C
UniRef100_P81102

Bacillus subtilis

YodC

[Bacillus subtilis]

2172
YolF [Bacillus subtilis]
UniRef100_O34842

Bacillus subtilis

YodD

2173
UPI0000315ACC UniRef100 entry
UniRef100_UPI0000315ACC

2174
YodF protein [Bacillus subtilis]
UniRef100_O34745

Bacillus subtilis

YodF

2175
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012

anthracis str. A2012]

2176
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069

2177
OrfRM1 [Bacillus subtilis]
UniRef100_O34666

Bacillus subtilis

CtpA

2178
YolB [Bacillus subtilis]
UniRef100_O34954

Bacillus subtilis

YodH

2179

2180
Carboxypeptidase [Bacillus subtilis]
UniRef100_O34866

Bacillus subtilis

YodJ

2181
Purine nucleoside phosphorylase II
UniRef100_O34925

Bacillus subtilis

DeoD

[Bacillus subtilis]

2182
Hypothetical Membrane Spanning
UniRef100_Q813P0

Bacillus cereus

YcgR

Protein [Bacillus cereus]

2183
YcgQ protein [Bacillus subtilis]
UniRef100_P94394

Bacillus subtilis

YcgQ

2184

2185
Hypothetical protein yodL [Bacillus
UniRef100_O30472

Bacillus subtilis

YodL

subtilis]

2186

YodM

2187
Hypothetical protein yozD [Bacillus
UniRef100_O31863

Bacillus subtilis

subtilis]

2188
Hypothetical protein yodN [Bacillus
UniRef100_O34414

Bacillus subtilis

YodN

subtilis]

2189

2190
YokU [Bacillus subtilis]
UniRef100_O30470

Bacillus subtilis

2191
Hypothetical UPF0069 protein yodO
UniRef100_O34676

Bacillus subtilis

KamA

[Bacillus subtilis]

2192
YodP [Bacillus subtilis]
UniRef100_O34895

Bacillus subtilis

YodP

2193
Acetylornitine deacetylase [Bacillus
UniRef100_O34984

Bacillus subtilis

YodQ

subtilis]

2194
Butirate-acetoacetate CoA transferase
UniRef100_O34466

Bacillus subtilis

YodR

[Bacillus subtilis]

2195
Butyrate acetoacetate-CoA transferase
UniRef100_O34317

Bacillus subtilis

YodS

[Bacillus subtilis]

2196
Probable aminotransferase yodT
UniRef100_O34662

Bacillus subtilis

YodT

[Bacillus subtilis]

2197
Multidrug resistance protein; possible
UniRef100_Q6HK46

Bacillus

YkuC

tetracycline resistance determinant

thuringiensis

[Bacillus thuringiensis]

2198
Protein cgeE [Bacillus subtilis]
UniRef100_P42093

Bacillus subtilis

CgeE

2199
Peptide methionine sulfoxide reductase
UniRef100_P54155

Bacillus subtilis

YppQ

msrB [Bacillus subtilis]

2200

MsrA

2201
Putative HTH-type transcriptional
UniRef100_P54182

Bacillus subtilis

YpoP

regulator ypoP [Bacillus subtilis]

2202

2203
Hypothetical protein yhcK [Bacillus
UniRef100_P54595

Bacillus subtilis

YhcK

subtilis]

2204
Hypothetical protein ypnP [Bacillus
UniRef100_P54181

Bacillus subtilis

YpnP

subtilis]

2205
Hypothetical conserved protein
UniRef100_Q746K9

Thermus thermophilus

[Thermus thermophilus]

2206

2207
Hypothetical protein ypmS [Bacillus
UniRef100_P54179

Bacillus subtilis

YpmS

subtilis]

2208
Hypothetical protein ypmR [Bacillus
UniRef100_P40766

Bacillus subtilis

YpmR

subtilis]

2209
Hypothetical protein ypmQ [Bacillus
UniRef100_P54178

Bacillus subtilis

YpmQ

subtilis]

2210
DegV family protein [Bacillus cereus
UniRef100_Q63BU6

Bacillus cereus

YviA

ZK]

ZK

2211
Hypothetical protein ypmP [Bacillus
UniRef100_P54177

Bacillus subtilis

subtilis]

2212
Threonine dehydratase biosynthetic
UniRef100_P37946

Bacillus subtilis

IlvA

[Bacillus subtilis]

2213
Putative sigma L-dependent
UniRef100_P54156

Bacillus subtilis

YplP

transcriptional regulator yplP [Bacillus

subtilis]

2214
Hemolysin III homolog [Bacillus subtilis]
UniRef100_P54175

Bacillus subtilis

YplQ

2215
Hypothetical protein ypkP [Bacillus
UniRef100_P54174

Bacillus subtilis

YpkP

subtilis]

2216
Dihydrofolate reductase [Bacillus
UniRef100_P11045

Bacillus subtilis

DfrA

subtilis]

2217
Hypothetical protein ypjQ [Bacillus
UniRef100_P54173

Bacillus subtilis

YpjQ

subtilis]

2218

YpjP

2219

YpjP

2220
Hypothetical protein yphP [Bacillus
UniRef100_P54170

Bacillus subtilis

YphP

subtilis]

2221
Dihydroxy-acid dehydratase [Bacillus
UniRef100_P51785

Bacillus subtilis

IlvD

subtilis]

2222

YpgR

2223
Hypothetical protein ypgQ [Bacillus
UniRef100_P54168

Bacillus subtilis

YpgQ

subtilis]

2224
Glutathione peroxidase homolog bsaA
UniRef100_P52035

Bacillus subtilis

BsaA

[Bacillus subtilis]

2225
UPI00003CBA0F UniRef100 entry
UniRef100_UPI00003CBA0F

2226
Homoserine O-succinyltransferase
UniRef100_P54167

Bacillus subtilis

MetA

[Bacillus subtilis]

2227
Putative glycosyl transferase ypfP
UniRef100_P54166

Bacillus subtilis

UgtP

[Bacillus subtilis]

2228

2229
Cold shock protein cspD [Bacillus
UniRef100_P51777

Bacillus subtilis

subtilis]

2230
Regulatory protein degR [Bacillus
UniRef100_P06563

Bacillus subtilis

subtilis]

2231
Hypothetical protein ypzA [Bacillus
UniRef100_O32007

Bacillus subtilis

subtilis]

2232

2233
Hypothetical protein ypeP [Bacillus
UniRef100_P54164

Bacillus subtilis

YpeP

subtilis]

2234
Hypothetical protein ypdP [Bacillus
UniRef100_P54163

Bacillus subtilis

YpdP

subtilis]

2235
14.7 kDa ribonuclease H-like protein
UniRef100_P54162

Bacillus subtilis

YpdQ

[Bacillus subtilis]

2236
Probable 5′-3′ exonuclease [Bacillus
UniRef100_P54161

Bacillus subtilis

YpcP

subtilis]

2237

2238
Hypothetical protein ypbS [Bacillus
UniRef100_P54160

Bacillus subtilis

subtilis]

2239
Hypothetical protein ypbR [Bacillus
UniRef100_P54159

Bacillus subtilis

YpbR

subtilis]

2240

2241
Hypothetical protein ypbQ [Bacillus
UniRef100_P54158

Bacillus subtilis

YpbQ

subtilis]

2242

BcsA

2243
Predicted acetyltransferase [Clostridium
UniRef100_Q97G03

Clostridium

YokL

acetobutylicum]

acetobutylicum

2244
Xanthine permease [Bacillus subtilis]
UniRef100_P42086

Bacillus subtilis

PbuX

2245
Xanthine phosphoribosyltransferase
UniRef100_P42085

Bacillus subtilis

Xpt

[Bacillus subtilis]

2246
Hypothetical metalloprotease ypwA
UniRef100_P50848

Bacillus subtilis

YpwA

[Bacillus subtilis]

2247

YpvA

2248
Hypothetical protein yptA precursor
UniRef100_P50841

Bacillus subtilis

[Bacillus subtilis]

2249

2250
Hypothetical UPF0020 protein ypsC
UniRef100_P50840

Bacillus subtilis

YpsC

precursor [Bacillus subtilis]

2251
Hypothetical protein ypsB [Bacillus
UniRef100_P50839

Bacillus subtilis

subtilis]

2252
Hypothetical protein ypsA [Bacillus
UniRef100_P50838

Bacillus subtilis

YpsA

subtilis]

2253
Spore coat protein D [Bacillus subtilis]
UniRef100_P07791

Bacillus subtilis

2254

2255

YprB

2256

YprA

2257
Putative PTS system IIA component
UniRef100_P50829

Bacillus subtilis

YpqE

ypqE [Bacillus subtilis]

2258
Hypothetical protein ypqA precursor
UniRef100_P50836

Bacillus subtilis

YpqA

[Bacillus subtilis]

2259
Hypothetical protein yppG [Bacillus
UniRef100_P50835

Bacillus subtilis

YppG

subtilis]

2260

2261

YppE

2262

2263

2264
Hypothetical protein yppC [Bacillus
UniRef100_P39791

Bacillus subtilis

YppC

subtilis]

2265
Recombination protein U [Bacillus
UniRef100_P39792

Bacillus subtilis

RecU

subtilis]

2266
Penicillin-binding protein 1A/1B (PBP1)
UniRef100_P39793
Includes:
PonA

[Includes: Penicillin-insensitive

Penicillin-

transglycosylase (EC 2.4.2.—)

insensitive

(Peptidoglycan TGase); Penicillin-

transglycosylase

sensitive transpeptidase (EC 3.4.—.—)

(EC 2.4.2.—)

(DD-transpeptidase)] [Bacillus subtilis]

(Peptidoglycan

TGase);

Penicillin-

sensitive

transpeptidase

(EC 3.4.—.—) (DD-

transpeptidase)

2267
Hypothetical protein ypoC [Bacillus
UniRef100_P39789

Bacillus subtilis

YpoC

subtilis]

2268
Probable endonuclease III (EC
UniRef100_P39788

Bacillus subtilis

Nth

4.2.99.18) (DNA-(apurinic or

apyrimidinic site) lyase) [Bacillus

subtilis]

2269
DNA replication protein dnaD [Bacillus
UniRef100_P39787

Bacillus subtilis

DnaD

subtilis]

2270
Asparaginyl-tRNA synthetase [Bacillus
UniRef100_P39772

Bacillus subtilis

AsnS

subtilis]

2271
Aspartate aminotransferase [Bacillus
UniRef100_P53001

Bacillus subtilis

AspB

subtilis]

2272
Hypothetical protein ypmB [Bacillus
UniRef100_P54396

Bacillus subtilis

YpmB

subtilis]

2273
Hypothetical protein ypmA [Bacillus
UniRef100_P54395

Bacillus subtilis

subtilis]

2274
Probable ATP-dependent helicase dinG
UniRef100_P54394

Bacillus subtilis

DinG

homolog [Bacillus subtilis]

2275
Aspartate 1-decarboxylase precursor
UniRef100_P52999

Bacillus subtilis

PanD

[Bacillus subtilis]

2276
Pantoate--beta-alanine ligase [Bacillus
UniRef100_P52998

Bacillus subtilis

PanC

subtilis]

2277
3-methyl-2-oxobutanoate
UniRef100_P52996

Bacillus subtilis

PanB

hydroxymethyltransferase [Bacillus

subtilis]

2278
BirA bifunctional protein [Includes:
UniRef100_P42975
Includes: Biotin
BirA

Biotin operon repressor; Biotin--[acetyl-

operon

CoA-carboxylase] synthetase (EC

repressor; Biotin--

6.3.4.15) (Biotin--protein ligase)]

[Bacillus subtilis]

2279
Poly(A) polymerase [Bacillus subtilis]
UniRef100_P42977

Bacillus subtilis

Cca

2280
Putative glycosyl transferase ypjH
UniRef100_P42982

Bacillus subtilis

YpjH

[Bacillus subtilis]

2281
Hypothetical protein ypjG [Bacillus
UniRef100_P42981

Bacillus subtilis

YpjG

subtilis]

2282
Methylglyoxal synthase [Bacillus
UniRef100_P42980

Bacillus subtilis

MgsA

subtilis]

2283
Dihydrodipicolinate reductase [Bacillus
UniRef100_P42976

Bacillus subtilis

DapB

subtilis]

2284
Hypothetical protein ypjD [Bacillus
UniRef100_P42979

Bacillus subtilis

YpjD

subtilis]

2285

YpjC

2286
Hypothetical protein ypjB precursor
UniRef100_P54393

Bacillus subtilis

YpjB

[Bacillus subtilis]

2287

YpjA

2288

QcrC

2289
Menaquinol-cytochrome c reductase
UniRef100_P46912

Bacillus subtilis

QcrB

cytochrome b subunit [Bacillus subtilis]

2290
Menaquinol-cytochrome c reductase
UniRef100_P46911

Bacillus subtilis

QcrA

iron-sulfur subunit [Bacillus subtilis]

2291
Hypothetical protein ypiF [Bacillus
UniRef100_P54391

Bacillus subtilis

YpiF

subtilis]

2292
Hypothetical UPF0302 protein ypiB
UniRef100_P54390

Bacillus subtilis

YpiB

[Bacillus subtilis]

2293
Hypothetical protein ypiA [Bacillus
UniRef100_P54389

Bacillus subtilis

YpiA

subtilis]

2294
3-phosphoshikimate 1-
UniRef100_P20691

Bacillus subtilis

AroE

carboxyvinyltransferase [Bacillus

subtilis]

2295
Prephenate dehydrogenase [Bacillus
UniRef100_P20692

Bacillus subtilis

TyrA

subtilis]

2296

HisC

2297
Tryptophan synthase alpha chain
UniRef100_P07601

Bacillus subtilis

TrpA

[Bacillus subtilis]

2298
Tryptophan synthase beta chain
UniRef100_P07600

Bacillus subtilis

TrpB

[Bacillus subtilis]

2299
N-(5′-phosphoribosyl)anthranilate
UniRef100_P20167

Bacillus subtilis

TrpF

isomerase [Bacillus subtilis]

2300
Indole-3-glycerol phosphate synthase
UniRef100_P03964

Bacillus subtilis

TrpC

[Bacillus subtilis]

2301
Anthranilate phosphoribosyltransferase
UniRef100_P03947

Bacillus subtilis

TrpD

[Bacillus subtilis]

2302
Anthranilate synthase component I
UniRef100_P03963

Bacillus subtilis

TrpE

[Bacillus subtilis]

2303
Chorismate mutase [Bacillus subtilis]
UniRef100_P19080

Bacillus subtilis

AroH

2304
3-dehydroquinate synthase [Bacillus
UniRef100_P31102

Bacillus subtilis

AroB

subtilis]

2305
Chorismate synthase [Bacillus subtilis]
UniRef100_P31104

Bacillus subtilis

AroF

2306
Chemotaxis protein methyltransferase
UniRef100_P31105

Bacillus subtilis

CheR

[Bacillus subtilis]

2307
Nucleoside diphosphate kinase
UniRef100_P31103

Bacillus subtilis

Ndk

[Bacillus subtilis]

2308
Heptaprenyl diphosphate synthase
UniRef100_P31114

Bacillus subtilis

HepT

component II [Bacillus subtilis]

2309
Menaquinone biosynthesis
UniRef100_P31113

Bacillus subtilis

MenH

methyltransferase ubiE [Bacillus

subtilis]

2310
Heptaprenyl diphosphate synthase
UniRef100_P31112

Bacillus subtilis

HepS

component I [Bacillus subtilis]

2311
Transcription attenuation protein mtrB
UniRef100_P19466

Bacillus subtilis

[Bacillus subtilis]

2312
GTP cyclohydrolase I [Bacillus subtilis]
UniRef100_P19465

Bacillus subtilis

MtrA

2313
DNA-binding protein HU 1 [Bacillus
UniRef100_P08821

Bacillus subtilis

subtilis]

2314
Stage IV sporulation protein A [Bacillus
UniRef100_P35149

Bacillus subtilis

SpoIVA

subtilis]

2315
Hypothetical protein yphF [Bacillus
UniRef100_P39911

Bacillus subtilis

YphF

subtilis]

2316
Hypothetical protein yphE [Bacillus
UniRef100_P50744

Bacillus subtilis

subtilis]

2317
Glycerol-3-phosphate dehydrogenase
UniRef100_P46919
NAD(P)+
GpsA

[NAD(P)+] (EC 1.1.1.94) (NAD(P)H-

dependent glycerol-3-phosphate

dehydrogenase) (NAD(P)H-dependent

dihydroxyacetone-phosphate

reductase) [Bacillus subtilis]

2318

YphC

2319
Hypothetical protein OB1798
UniRef100_Q8EQA7

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

2320
Hypothetical protein yphB [Bacillus
UniRef100_P50742

Bacillus subtilis

SeaA

subtilis]

2321
Hypothetical protein yphA [Bacillus
UniRef100_P50741

Bacillus subtilis

YphA

subtilis]

2322

2323

YpgA

2324
30S ribosomal protein S1 homolog
UniRef100_P38494

Bacillus subtilis

YpfD

[Bacillus subtilis]

2325
Cytidylate kinase [Bacillus subtilis]
UniRef100_P38493

Bacillus subtilis

Cmk

2326
Hypothetical protein ypfB [Bacillus
UniRef100_P38492

Bacillus subtilis

subtilis]

2327
Sporulation protein ypeB [Bacillus
UniRef100_P38490

Bacillus subtilis

YpeB

subtilis]

2328
Spore cortex-lytic enzyme precursor
UniRef100_P50739

Bacillus subtilis

SleB

[Bacillus subtilis]

2329

YpdC

2330

YccC

2331
Hypothetical protein ypdA [Bacillus
UniRef100_P50736

Bacillus subtilis

YpdA

subtilis]

2332
NAD-specific glutamate dehydrogenase
UniRef100_P50735

Bacillus subtilis

GudB

[Bacillus subtilis]

2333
Adapter protein mecA 2 [Bacillus
UniRef100_P50734

Bacillus subtilis

YpbH

subtilis]

2334
Hypothetical protein ypbG precursor
UniRef100_P50733

Bacillus subtilis

YpbG

[Bacillus subtilis]

2335

YpbF

2336
Hypothetical protein ypbE [Bacillus
UniRef100_P50731

Bacillus subtilis

YpbE

subtilis]

2337
Hypothetical protein ypbD [Bacillus
UniRef100_P50730

Bacillus subtilis

YpbD

subtilis]

2338
ATP-dependent DNA helicase recQ
UniRef100_P50729

Bacillus subtilis

RecQ

[Bacillus subtilis]

2339
Hypothetical protein ypbB [Bacillus
UniRef100_P50728

Bacillus subtilis

YpbB

subtilis]

2340
Ferredoxin [Bacillus subtilis]
UniRef100_P50727

Bacillus subtilis

2341
Hypothetical protein ypaA [Bacillus
UniRef100_P50726

Bacillus subtilis

YpaA

subtilis]

2342

2343
D-3-phosphoglycerate dehydrogenase
UniRef100_P35136

Bacillus subtilis

SerA

[Bacillus subtilis]

2344
BH1600 protein [Bacillus halodurans]
UniRef100_Q9KCH1

Bacillus halodurans

2345
Sigma-X negative effector [Bacillus
UniRef100_P35166

Bacillus subtilis

RsiX

subtilis]

2346
RNA polymerase sigma factor sigX
UniRef100_P35165

Bacillus subtilis

SigX

[Bacillus subtilis]

2347
Transcriptional regulator [Bacillus
UniRef100_Q72XJ3

Bacillus cereus

LytR

cereus]

2348
Endo-1,4-beta-xylanase [Bacillus
UniRef100_Q9K630

Bacillus

YheN

halodurans]

halodurans

2349

2350
Alkaline phosphatase synthesis sensor
UniRef100_Q898N3

Clostridium tetani

YclK

protein phoR [Clostridium tetani]

2351
Response regulators consisting of a
UniRef100_Q8R9H7

Thermoanaerobacter

YycF

CheY-like receiver domain and a HTH

tengcongensis

DNA-binding domain

[Thermoanaerobacter tengcongensis]

2352
Sensor protein resE [Bacillus subtilis]
UniRef100_P35164

Bacillus subtilis

ResE

2353
Transcriptional regulatory protein resD
UniRef100_P35163

Bacillus subtilis

ResD

[Bacillus subtilis]

2354
Protein resC [Bacillus subtilis]
UniRef100_P35162

Bacillus subtilis

ResC

2355
ResB protein [Bacillus subtilis]
UniRef100_P35161

Bacillus subtilis

ResB

2356
Thiol-disulfide oxidoreductase resA
UniRef100_P35160

Bacillus subtilis

ResA

[Bacillus subtilis]

2357
Ribosomal large subunit pseudouridine
UniRef100_P35159

Bacillus subtilis

RluB

synthase B [Bacillus subtilis]

2358
Spore maturation protein B [Bacillus
UniRef100_P35158

Bacillus subtilis

SpmB

subtilis]

2359
Spore maturation protein A [Bacillus
UniRef100_P35157

Bacillus subtilis

SpmA

subtilis]

2360
Penicillin-binding protein 5* precursor
UniRef100_P35150

Bacillus subtilis

DacB

[Bacillus subtilis]

2361
Hypothetical protein ypul [Bacillus
UniRef100_P35156

Bacillus subtilis

YpuI

subtilis]

2362
Segregation and condensation protein
UniRef100_P35155

Bacillus subtilis

YpuH

B [Bacillus subtilis]

2363
Segregation and condensation protein
UniRef100_P35154

Bacillus subtilis

YpuG

A [Bacillus subtilis]

2364

2365
Hypothetical protein ypuF [Bacillus
UniRef100_P17617

Bacillus subtilis

YpuF

subtilis]

2366
RibT protein [Bacillus subtilis]
UniRef100_P17622

Bacillus subtilis

RibT

2367
6,7-dimethyl-8-ribityllumazine synthase
UniRef100_Q44681

Bacillus

RibH

[Bacillus amyloliquefaciens]

amyloliquefaciens

2368
Riboflavin biosynthesis protein ribA
UniRef100_P17620
Includes: GTP
RibA

[Includes: GTP cyclohydrolase II (EC

cyclohydrolase II

3.5.4.25); 3,4-dihydroxy-2-butanone 4-

(EC 3.5.4.25);

phosphate synthase (DHBP synthase)]

3,4-dihydroxy-2-

[Bacillus subtilis]

butanone 4-

phosphate

synthase (DHBP

synthase)

2369
Riboflavin synthase alpha chain
UniRef100_P16440

Bacillus subtilis

RibE

[Bacillus subtilis]

2370
Riboflavin biosynthesis protein ribD
UniRef100_P17618
Includes:
RibD

[Includes:

Diaminohydroxyphosphoribosyl-

Diaminohydroxyphosphoribosylamino-

aminopyrimidine deaminase

pyrimidine deaminase (EC 3.5.4.26)

(EC 3.5.4.26)

(Riboflavin-specific deaminase); 5-

(Riboflavin-

amino-6-(5-phosphoribosylamino)uracil

specific

reductase (EC 1.1.1.193) (HTP

deaminase); 5-

reductase)] [Bacillus subtilis]

amino-6-(5-

phosphoribosylamino)uracil

reductase (EC

1.1.1.193) (HTP

reductase)

2371
Hypothetical protein ypuD [Bacillus
UniRef100_P17616

Bacillus subtilis

YpuD

subtilis]

2372
Putative serine/threonine protein
UniRef100_Q7VFC1

Helicobacter

YjbP

phosphatase [Helicobacter hepaticus]

hepaticus

2373
Stress response homolog Hsp [Bacillus
UniRef100_Q9X3Z5

Bacillus subtilis

subtilis]

2374
Response regulator aspartate
UniRef100_Q00828

Bacillus subtilis

RapA

phosphatase A [Bacillus subtilis]

2375
Peptidyl-prolyl cis-trans isomerase B
UniRef100_P35137

Bacillus subtilis

PpiB

[Bacillus subtilis]

2376

YpuA

2377
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012

anthracis str. A2012]

2378
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069

2379

YndL

2380
Diaminopimelate decarboxylase
UniRef100_P23630

Bacillus subtilis

LysA

[Bacillus subtilis]

2381

2382
Stage V sporulation protein AF [Bacillus
UniRef100_P31845

Bacillus subtilis

SpoVAF

subtilis]

2383
Stage V sporulation protein AE [Bacillus
UniRef100_P40870

Bacillus subtilis

SpoVAE

subtilis]

2384
Stage V sporulation protein AE [Bacillus
UniRef100_P40870

Bacillus subtilis

SpoVAE

subtilis]

2385
Stage V sporulation protein AD
UniRef100_P40869

Bacillus subtilis

SpoVAD

[Bacillus subtilis]

2386

SpoVAC

2387
Stage V sporulation protein AB [Bacillus
UniRef100_P40867

Bacillus subtilis

SpoVAB

subtilis]

2388
Stage V sporulation protein AA [Bacillus
UniRef100_P40866

Bacillus subtilis

SpoVAA

subtilis]

2389

SigF

2390

SpoIIAB

2391

SpoIIAA

2392
Penicillin-binding protein dacF
UniRef100_P38422

Bacillus subtilis

DacF

precursor [Bacillus subtilis]

2393
Purine nucleoside phosphorylase I
UniRef100_P46354

Bacillus subtilis

PunA

[Bacillus subtilis]

2394
Phosphopentomutase [Bacillus subtilis]
UniRef100_P46353

Bacillus subtilis

Drm

2395
Tyrosine recombinase xerD [Bacillus
UniRef100_P46352

Bacillus subtilis

RipX

subtilis]

2396

2397
Ferric uptake regulation protein
UniRef100_P54574

Bacillus subtilis

Fur

[Bacillus subtilis]

2398
Stage II sporulation protein M [Bacillus
UniRef100_P37873

Bacillus subtilis

SpoIIM

subtilis]

2399
Lmo2763 protein [Listeria
UniRef100_Q926Y4

Listeria

YdhO

monocytogenes]

monocytogenes

2400
UPI00003CA2F1 UniRef100 entry
UniRef100_UPI00003CA2F1

2401
Probable allantoin permease [Bacillus
UniRef100_P94575

Bacillus subtilis

YwoE

subtilis]

2402

YdaF

2403
Hypothetical protein yqkK [Bacillus
UniRef100_P54573

Bacillus subtilis

subtilis]

2404

YybD

2405
Hypothetical protein [Bacillus pumilus]
UniRef100_Q93PN4

Bacillus pumilus

YqxK

2406
ADP-ribose pyrophosphatase [Bacillus
UniRef100_P54570

Bacillus subtilis

NudF

subtilis]

2407

2408
YdgC protein [Bacillus subtilis]
UniRef100_P96701

Bacillus subtilis

YdgC

2409
Hypothetical protein ydgD [Bacillus
UniRef100_P96702

Bacillus subtilis

YdgD

subtilis]

2410
Hypothetical oxidoreductase yqkF
UniRef100_P54569

Bacillus subtilis

YqkF

[Bacillus subtilis]

2411

2412

YqkD

2413
Hypothetical protein yqkC [Bacillus
UniRef100_P54566

Bacillus subtilis

subtilis]

2414
Hypothetical protein yqkB [Bacillus
UniRef100_P54565

Bacillus subtilis

YqkB

subtilis]

2415
UPI00003CBE2B UniRef100 entry
UniRef100_UPI00003CBE2B

2416
Hypothetical UPF0157 protein yqkA
UniRef100_P54564

Bacillus subtilis

YqkA

[Bacillus subtilis]

2417
Hypothetical protein yqjZ [Bacillus
UniRef100_P54563

Bacillus subtilis

YqjZ

subtilis]

2418
Lipase precursor [Bacillus subtilis]
UniRef100_P37957

Bacillus subtilis

Lip

2419

2420
Nickel ABC transporter [Bacillus
UniRef100_Q9KBX8

Bacillus

AppA

halodurans]

halodurans

2421
Nickel ABC transporter [Bacillus
UniRef100_Q9KBX7

Bacillus

AppB

halodurans]

halodurans

2422
Nickel ABC transporter [Bacillus
UniRef100_Q9KBX6

Bacillus

AppC

halodurans]

halodurans

2423
Putative oligopeptide ABC transporter
UniRef100_Q895A4

Clostridium tetani

DppD

[Clostridium tetani]

2424
Putative oligopeptide ABC transporter
UniRef100_Q895A5

Clostridium tetani

OppF

[Clostridium tetani]

2425
DNA-damage-inducible protein [Bacillus
UniRef100_Q9KDR1

Bacillus

YojI

halodurans]

halodurans

2426
Fibronectin-binding protein [Bacillus
UniRef100_Q63CW1

Bacillus cereus ZK

cereus ZK]

2427
Hypothetical protein yolD
UniRef100_O64030
Bacteriophage
YolD

[Bacteriophage SPBc2]

SPBc2

2428

UvrX

2429
Hypothetical protein yqzH [Bacillus
UniRef100_O32014

Bacillus subtilis

subtilis]

2430
Hypothetical transport protein yqjV
UniRef100_P54559

Bacillus subtilis

YqjV

[Bacillus subtilis]

2431
Hypothetical protein yqjT [Bacillus
UniRef100_P54557

Bacillus subtilis

YqjT

subtilis]

2432

CoaA

2433
LacG [Lactococcus lactis]
UniRef100_Q9RAU9

Lactococcus lactis

2434
LacF [Lactococcus lactis]
UniRef100_Q9RAV2

Lactococcus

YdbJ

lactis

2435

2436
Hypothetical protein [Bacillus
UniRef100_Q70K07

Bacillus amyloliquefaciens

amyloliquefaciens]

2437
Hypothetical protein [Bacillus cereus]
UniRef100_Q737G3

Bacillus cereus

2438
YmaC protein [Bacillus subtilis]
UniRef100_O31789

Bacillus subtilis

YmaC

2439
Hypothetical oxidoreductase yqjQ
UniRef100_P54554

Bacillus subtilis

YqjQ

[Bacillus subtilis]

2440

YqjP

2441

ProI

2442
Hypothetical protein yqjN [Bacillus
UniRef100_P54551

Bacillus subtilis

YqjN

subtilis]

2443
Probable NADH-dependent flavin
UniRef100_P54550

Bacillus subtilis

YqjM

oxidoreductase yqjM [Bacillus subtilis]

2444
Hypothetical protein yqjL [Bacillus
UniRef100_P54549

Bacillus subtilis

YqjL

subtilis]

2445

2446
Ribonuclease Z [Bacillus subtilis]
UniRef100_P54548

Bacillus subtilis

YqjK

2447
Glucose-6-phosphate 1-dehydrogenase
UniRef100_P54547

Bacillus subtilis

Zwf

[Bacillus subtilis]

2448
Hypothetical conserved protein
UniRef100_Q8ELZ9

Oceanobacillus

CitH

[Oceanobacillus iheyensis]

iheyensis

2449
Hypothetical protein OB3065
UniRef100_Q8ELZ8

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

2450
Immunogenic protein [Oceanobacillus
UniRef100_Q8ELZ7

Oceanobacillus

SsuA

iheyensis]

iheyensis

2451
YdhQ protein [Bacillus subtilis]
UniRef100_O05509

Bacillus subtilis

YdhQ

2452
Beta-glucosidase [Bacillus halodurans]
UniRef100_Q9K615

Bacillus

YdhP

halodurans

2453
Putative cellobiose-specific enzyme IIC
UniRef100_Q8KP28

Bacillus pumilus

YdhO

[Bacillus pumilus]

2454
YdhO protein [Bacillus subtilis]
UniRef100_O05507

Bacillus subtilis

YdhO

2455
YdhN protein [Bacillus subtilis]
UniRef100_O05506

Bacillus subtilis

YdhN

2456
PTS system, cellobiose-specific
UniRef100_Q9K613

Bacillus halodurans

enzyme II, B component [Bacillus

halodurans]

2457
Alkaline phosphatase [Bacillus cereus
UniRef100_Q639W1

Bacillus cereus

PhoB

ZK]

ZK

2458
Glucose 1-dehydrogenase A [Bacillus
UniRef100_P10528

Bacillus

Gdh

megaterium]

megaterium

2459
6-phosphogluconate dehydrogenase,
UniRef100_P80859

Bacillus subtilis

YqjI

decarboxylating II [Bacillus subtilis]

2460
DNA polymerase IV 1 [Bacillus subtilis]
UniRef100_P54545

Bacillus subtilis

YqjH

2461
Hypothetical protein yqzJ [Bacillus
UniRef100_Q7WY64

Bacillus subtilis

subtilis]

2462

YqjG

2463
Hypothetical protein yqjE [Bacillus
UniRef100_P54542

Bacillus subtilis

YqjE

subtilis]

2464
Methylmalonyl-CoA decarboxylase
UniRef100_Q9K8P6

Bacillus

YqjD

alpha subunit [Bacillus halodurans]

halodurans

2465
Hypothetical protein yqjA [Bacillus
UniRef100_P54538

Bacillus subtilis

YqjA

subtilis]

2466
Probable amino-acid ABC transporter
UniRef100_P54537

Bacillus subtilis

YqiZ

ATP-binding protein yqiZ [Bacillus

subtilis]

2467
Probable amino-acid ABC transporter
UniRef100_P54536

Bacillus subtilis

YqiY

permease protein yqiY [Bacillus subtilis]

2468
Probable amino-acid ABC transporter
UniRef100_P54535

Bacillus subtilis

YqiX

extracellular binding protein yqiX

precursor [Bacillus subtilis]

2469
Hypothetical protein yqiW [Bacillus
UniRef100_P54534

Bacillus subtilis

YqiW

subtilis]

2470
Protein bmrU [Bacillus subtilis]
UniRef100_P39074

Bacillus subtilis

BmrU

2471
Lipoamide acyltransferase component
UniRef100_P37942

Bacillus subtilis

BkdB

of branched-chain alpha-keto acid

dehydrogenase complex (EC 2.3.1.168)

(Dihydrolipoyllysine-residue (2-

methylpropanoyl)transferase) [Bacillus

subtilis]

2472
2-oxoisovalerate dehydrogenase beta
UniRef100_P37941

Bacillus subtilis

BkdAB

subunit [Bacillus subtilis]

2473
2-oxoisovalerate dehydrogenase alpha
UniRef100_P37940

Bacillus subtilis

BkdAA

subunit [Bacillus subtilis]

2474
Dihydrolipoyl dehydrogenase [Bacillus
UniRef100_P54533

Bacillus subtilis

LpdV

subtilis]

2475
Probable butyrate kinase [Bacillus
UniRef100_P54532

Bacillus subtilis

Buk

subtilis]

2476
Leucine dehydrogenase [Bacillus
UniRef100_P54531

Bacillus subtilis

Bcd

subtilis]

2477
Probable phosphate butyryltransferase
UniRef100_P54530

Bacillus subtilis

Ptb

[Bacillus subtilis]

2478
Putative sigma L-dependent
UniRef100_P54529

Bacillus subtilis

BkdR

transcriptional regulator yqiR [Bacillus

subtilis]

2479
Hypothetical protein yqzF [Bacillus
UniRef100_O32015

Bacillus subtilis

subtilis]

2480
Hypothetical protein yqiK [Bacillus
UniRef100_P54527

Bacillus subtilis

YqiK

subtilis]

2481
Hypothetical protein yqiI [Bacillus
UniRef100_P54525

Bacillus subtilis

YqiI

subtilis]

2482

YqiH

2483
Serine O-acetyltransferase
UniRef100_Q8PSY4

Methanosarcina

CysE

[Methanosarcina mazei]

mazei

2484

2485
Stage 0 sporulation protein A [Bacillus
UniRef100_P06534

Bacillus subtilis

Spo0A

subtilis]

2486

SpoIVB

2487
DNA repair protein recN [Bacillus
UniRef100_Q659H4

Bacillus

RecN

amyloliquefaciens]

amyloliquefaciens

2488
Arginine repressor [Bacillus subtilis]
UniRef100_P17893

Bacillus subtilis

AhrC

2489
Hypothetical protein yqxC [Bacillus
UniRef100_P19672

Bacillus subtilis

YqxC

subtilis]

2490

Dxs

2491
Geranyltranstransferase [Bacillus
UniRef100_P54383

Bacillus subtilis

YqiD

subtilis]

2492
Probable exodeoxyribonuclease VII
UniRef100_P54522

Bacillus subtilis

small subunit [Bacillus subtilis]

2493
Probable exodeoxyribonuclease VII
UniRef100_P54521

Bacillus subtilis

YqiB

large subunit [Bacillus subtilis]

2494
FolD bifunctional protein [Includes:
UniRef100_P54382
Includes:
FolD

Methylenetetrahydrofolate

Methylenetetrahydrofolate

dehydrogenase (EC 1.5.1.5);

dehydrogenase

Methenyltetrahydrofolate

(EC 1.5.1.5);

cyclohydrolase (EC 3.5.4.9)] [Bacillus

Methenyltetrahydrofolate

subtilis]

cyclohydrolase

(EC 3.5.4.9)

2495
N utilization substance protein B
UniRef100_P54520

Bacillus subtilis

NusB

homolog [Bacillus subtilis]

2496
Hypothetical protein yqhY [Bacillus
UniRef100_P54519

Bacillus subtilis

YqhY

subtilis]

2497

AccC

2498
Biotin carboxyl carrier protein of acetyl-
UniRef100_P49786

Bacillus subtilis

AccB

CoA carboxylase [Bacillus subtilis]

2499
Stage III sporulation protein AH
UniRef100_P49785

Bacillus subtilis

SpoIIIAH

[Bacillus subtilis]

2500
Stage III sporulation protein AG
UniRef100_P49784

Bacillus subtilis

SpoIIIAG

[Bacillus subtilis]

2501
Stage III sporulation protein AF
UniRef100_P49783

Bacillus subtilis

SpoIIIAF

[Bacillus subtilis]

2502
Stage III sporulation protein AE
UniRef100_P49782

Bacillus subtilis

SpoIIIAE

[Bacillus subtilis]

2503
Stage III sporulation protein AD
UniRef100_P49781

Bacillus subtilis

SpoIIIAD

[Bacillus subtilis]

2504
Stage III sporulation protein AC
UniRef100_P49780

Bacillus subtilis

[Bacillus subtilis]

2505
Stage III sporulation protein AB
UniRef100_Q01368

Bacillus subtilis

SpoIIIAB

[Bacillus subtilis]

2506

SpoIIIAA

2507
Hypothetical protein yqhV [Bacillus
UniRef100_P49779

Bacillus subtilis

subtilis]

2508
Elongation factor P [Bacillus subtilis]
UniRef100_P49778

Bacillus subtilis

Efp

2509
Putative peptidase yqhT [Bacillus
UniRef100_P54518

Bacillus subtilis

YqhT

subtilis]

2510
3-dehydroquinate dehydratase [Bacillus
UniRef100_P54517

Bacillus subtilis

YqhS

subtilis]

2511
Hypothetical protein yqhR [Bacillus
UniRef100_P54516

Bacillus subtilis

YqhR

subtilis]

2512
Hypothetical protein yqhQ [Bacillus
UniRef100_P54515

Bacillus subtilis

YqhQ

subtilis]

2513
Hypothetical protein yqhP [Bacillus
UniRef100_P54514

Bacillus subtilis

YqhP

subtilis]

2514
Hypothetical protein yqhO [Bacillus
UniRef100_P54513

Bacillus subtilis

YqhO

subtilis]

2515
Transcriptional regulator mntR [Bacillus
UniRef100_P54512

Bacillus subtilis

MntR

subtilis]

2516
Hypothetical protein yqhM [Bacillus
UniRef100_P54511

Bacillus subtilis

YqhM

subtilis]

2517
Hypothetical protein yqhL [Bacillus
UniRef100_P54510

Bacillus subtilis

YqhL

subtilis]

2518
Glycine betaine-binding protein
UniRef100_P46922

Bacillus subtilis

OpuAC

precursor [Bacillus subtilis]

2519
Glycine betaine transport system
UniRef100_P46921

Bacillus subtilis

OpuAB

permease protein opuAB [Bacillus

subtilis]

2520
Glycine betaine transport ATP-binding
UniRef100_P46920

Bacillus subtilis

OpuAA

protein opuAA [Bacillus subtilis]

2521
Probable glycine dehydrogenase
UniRef100_P54377
decarboxylating
GcvPB

[decarboxylating] subunit 2 [Bacillus

subtilis]

2522
Probable glycine dehydrogenase
UniRef100_P54376
decarboxylating
GcvPA

[decarboxylating] subunit 1 [Bacillus

subtilis]

subtilis]

2523
Aminomethyltransferase [Bacillus
UniRef100_P54378

Bacillus subtilis

GcvT

subtilis]

2524
Hypothetical helicase yqhH [Bacillus
UniRef100_P54509

Bacillus subtilis

YqhH

subtilis]

2525
Hypothetical protein yqhG [Bacillus
UniRef100_P54508

Bacillus subtilis

YqhG

subtilis]

2526

2527

SinR

2528
Spore coat-associated protein N
UniRef100_P54507

Bacillus subtilis

TasA

[Bacillus subtilis]

2529
Signal peptidase I W [Bacillus subtilis]
UniRef100_P54506

Bacillus subtilis

SipW

2530
Hypothetical protein yqxM [Bacillus
UniRef100_P40949

Bacillus subtilis

YqxM

subtilis]

2531
YqzG protein [Bacillus subtilis]
UniRef100_O32019

Bacillus subtilis

YqzG

2532
YqzE protein [Bacillus subtilis]
UniRef100_O32020

Bacillus subtilis

2533

ComGG

2534

ComGF

2535
ComG operon protein 5 precursor
UniRef100_P25957

Bacillus subtilis

ComGE

[Bacillus subtilis]

2536
ComG operon protein 4 precursor
UniRef100_P25956

Bacillus subtilis

ComGD

[Bacillus subtilis]

2537
ComG operon protein 3 precursor
UniRef100_P25955

Bacillus subtilis

[Bacillus subtilis]

2538

ComGB

2539
ComG operon protein 1 [Bacillus
UniRef100_P25953

Bacillus subtilis

ComGA

subtilis]

2540
Hypothetical protein yqhA [Bacillus
UniRef100_P54504

Bacillus subtilis

YqhA

subtilis]

2541
Hypothetical protein yqgZ [Bacillus
UniRef100_P54503

Bacillus subtilis

YqgZ

subtilis]

2542
Hypothetical protein yqgY [Bacillus
UniRef100_P54502

Bacillus subtilis

subtilis]

2543
Hypothetical protein yqgX [Bacillus
UniRef100_P54501

Bacillus subtilis

YqgX

subtilis]

2544
Hypothetical protein yqgW [Bacillus
UniRef100_P54500

Bacillus subtilis

subtilis]

2545
Hypothetical protein [Staphylococcus
UniRef100_Q8CSE8

Staphylococcus epidermidis

epidermidis]

2546

YqgU

2547
Hypothetical protein yqgT [Bacillus
UniRef100_P54497

Bacillus subtilis

YqgT

subtilis]

2548
Ferrichrome-binding protein precursor
UniRef100_P37580

Bacillus subtilis

FhuD

[Bacillus subtilis]

2549
Hypothetical protein yqgS [Bacillus
UniRef100_P54496

Bacillus subtilis

YqgS

subtilis]

2550
Glucokinase [Bacillus subtilis]
UniRef100_P54495

Bacillus subtilis

GlcK

2551
Hypothetical protein yqgQ [Bacillus
UniRef100_P54494

Bacillus subtilis

subtilis]

2552
Stage V sporulation protein AF
UniRef100_Q8EQ08

Oceanobacillus

SpoVAF

[Oceanobacillus iheyensis]

iheyensis

2553
Hypothetical protein yqgP [Bacillus
UniRef100_P54493

Bacillus subtilis

YqgP

subtilis]

2554
Hypothetical protein yqgO [Bacillus
UniRef100_P54492

Bacillus subtilis

subtilis]

2555
Hypothetical protein yqgN [Bacillus
UniRef100_P54491

Bacillus subtilis

YqgN

subtilis]

2556

2557

YqgM

2558
Hypothetical protein yqgL [Bacillus
UniRef100_P54489

Bacillus subtilis

YqgL

subtilis]

2559

YqzD

2560
YqzC protein [Bacillus subtilis]
UniRef100_O32023

Bacillus subtilis

YqzC

2561
Phosphate import ATP-binding protein
UniRef100_P46342

Bacillus subtilis

PstBB

pstB 1 [Bacillus subtilis]

2562
Phosphate import ATP-binding protein
UniRef100_P46341

Bacillus subtilis

PstBA

pstB 2 [Bacillus subtilis]

2563
Probable ABC transporter permease
UniRef100_P46340

Bacillus subtilis

PstA

protein yqqI [Bacillus subtilis]

2564
Probable ABC transporter permease
UniRef100_P46339

Bacillus subtilis

PstC

protein yqgH [Bacillus subtilis]

2565
Probable ABC transporter binding
UniRef100_P46338

Bacillus subtilis

PstS

protein yqgG precursor [Bacillus

subtilis]

2566
Hypothetical protein yqgF [Bacillus
UniRef100_P54488

Bacillus subtilis

PbpA

subtilis]

2567
Hypothetical protein yqgE [Bacillus
UniRef100_P54487

Bacillus subtilis

YqgE

subtilis]

2568

SodA

2569
Hypothetical protein yqgC [Bacillus
UniRef100_P54486

Bacillus subtilis

YqgC

subtilis]

2570
Hypothetical protein yqgB [Bacillus
UniRef100_P54485

Bacillus subtilis

YqgB

subtilis]

2571
Hypothetical protein yqfZ [Bacillus
UniRef100_P54483

Bacillus subtilis

subtilis]

2572
4-hydroxy-3-methylbut-2-en-1-yl
UniRef100_P54482

Bacillus subtilis

YqfY

diphosphate synthase (EC 1.17.4.3) (1-

hydroxy-2-methyl-2-(E)-butenyl 4-

diphosphate synthase) [Bacillus subtilis]

2573
Hypothetical protein yqfX [Bacillus
UniRef100_P54481

Bacillus subtilis

YqfX

subtilis]

2574
Putative nucleotidase yqfW [Bacillus
UniRef100_P54480

Bacillus subtilis

YqfW

subtilis]

2575
Zinc-specific metalloregulatory protein
UniRef100_P54479

Bacillus subtilis

Zur

[Bacillus subtilis]

2576
Metal (Zinc) transport protein [Listeria
UniRef100_Q926D9

Listeria innocua

YceA

innocua]

2577

YcdI

2578
Hypothetical protein yqfU [Bacillus
UniRef100_P54478

Bacillus subtilis

YqfU

subtilis]

2579

2580
Probable endonuclease IV [Bacillus
UniRef100_P54476

Bacillus subtilis

YqfS

subtilis]

2581
Probable RNA helicase yqfR [Bacillus
UniRef100_P54475

Bacillus subtilis

YqfR

subtilis]

2582
Hypothetical protein yqfQ [Bacillus
UniRef100_P54474

Bacillus subtilis

YqfQ

subtilis]

2583
4-hydroxy-3-methylbut-2-enyl
UniRef100_P54473

Bacillus subtilis

YqfP

diphosphate reductase [Bacillus

subtilis]

2584

YqfO

2585
Hypothetical protein yqfN [Bacillus
UniRef100_P54471

Bacillus subtilis

YqfN

subtilis]

2586
YwqL protein [Bacillus subtilis]
UniRef100_P96724

Bacillus subtilis

YwqL

2587

2588

2589

2590

2591
Hypothetical protein CAC0336
UniRef100_Q97M62

Clostridium acetobutylicum

[Clostridium acetobutylicum]

2592
Hypothetical protein [Bacillus
UniRef100_Q6HGF2

Bacillus thuringiensis

thuringiensis]

2593

2594
YwqJ protein [Bacillus subtilis]
UniRef100_P96722

Bacillus subtilis

YwqJ

2595
Hypothetical protein ywqI [Bacillus
UniRef100_P96721

Bacillus subtilis

subtilis]

2596

YwqH

2597
Cytochrome c-550 [Bacillus subtilis]
UniRef100_P24469

Bacillus subtilis

CccA

2598

SigA

2599
DNA primase [Bacillus subtilis]
UniRef100_P05096

Bacillus subtilis

DnaG

2600
Hypothetical UPF0178 protein yqxD
UniRef100_P17868

Bacillus subtilis

YqxD

[Bacillus subtilis]

2601
Hypothetical UPF0085 protein yqfL
UniRef100_P54470

Bacillus subtilis

YqfL

[Bacillus subtilis]

2602
YqzB protein [Bacillus subtilis]
UniRef100_O34994

Bacillus subtilis

YqzB

2603
Glycyl-tRNA synthetase beta chain
UniRef100_P54381

Bacillus subtilis

GlyS

[Bacillus subtilis]

2604
Glycyl-tRNA synthetase alpha chain
UniRef100_P54380

Bacillus subtilis

GlyQ

[Bacillus subtilis]

2605
DNA repair protein recO [Bacillus
UniRef100_P42095

Bacillus subtilis

RecO

subtilis]

2606

2607
GTP-binding protein era homolog
UniRef100_P42182

Bacillus subtilis

Era

[Bacillus subtilis]

2608
Cytidine deaminase [Bacillus subtilis]
UniRef100_P19079

Bacillus subtilis

Cdd

2609

2610
Hypothetical UPF0054 protein yqfG
UniRef100_P46347

Bacillus subtilis

YqfG

[Bacillus subtilis]

2611
Hypothetical protein yqfF [Bacillus
UniRef100_P46344

Bacillus subtilis

YqfF

subtilis]

2612
PhoH-like protein [Bacillus subtilis]
UniRef100_P46343

Bacillus subtilis

PhoH

2613

YqfD

2614
Hypothetical protein yqfC [Bacillus
UniRef100_P54468

Bacillus subtilis

subtilis]

2615
Hypothetical protein yqfB [Bacillus
UniRef100_P54467

Bacillus subtilis

YqfB

subtilis]

2616
Hypothetical protein yqfA [Bacillus
UniRef100_P54466

Bacillus subtilis

YqfA

subtilis]

2617

YqeZ

2618
Hypothetical protein yqeY [Bacillus
UniRef100_P54464

Bacillus subtilis

YqeY

subtilis]

2619

2620
Hypothetical protein yqeW [Bacillus
UniRef100_P54463

Bacillus subtilis

YqeW

subtilis]

2621
Deoxyribose-phosphate aldolase
UniRef100_Q92A19

Listeria innocua

Dra

[Listeria innocua]

2622
Hypothetical UPF0004 protein yqeV
UniRef100_P54462

Bacillus subtilis

YqeV

[Bacillus subtilis]

2623
Hypothetical UPF0088 protein yqeU
UniRef100_P54461

Bacillus subtilis

YqeU

[Bacillus subtilis]

2624
Ribosomal protein L11
UniRef100_P54460

Bacillus subtilis

YqeT

methyltransferase [Bacillus subtilis]

2625
Chaperone protein dnaJ [Bacillus
UniRef100_P17631

Bacillus subtilis

DnaJ

subtilis]

2626

DnaK

2627

2628

2629
Heat-inducible transcription repressor
UniRef100_P25499

Bacillus subtilis

HrcA

hrcA [Bacillus subtilis]

2630
Probable oxygen-independent
UniRef100_P54304

Bacillus subtilis

HemN

coproporphyrinogen III oxidase

[Bacillus subtilis]

2631

LepA

2632
Hypothetical protein yqxA [Bacillus
UniRef100_P38425

Bacillus subtilis

YqxA

subtilis]

2633

SpoIIP

2634
Germination protease precursor
UniRef100_P22322

Bacillus subtilis

Gpr

[Bacillus subtilis]

2635
30S ribosomal protein S20 [Bacillus
UniRef100_P21477

Bacillus subtilis

subtilis]

2636
Hypothetical protein yqeN [Bacillus
UniRef100_P54459

Bacillus subtilis

YqeN

subtilis]

2637

2638
ComE operon protein 3 [Bacillus
UniRef100_P39695

Bacillus subtilis

ComEC

subtilis]

2639
ComE operon protein 2 [Bacillus
UniRef100_P32393

Bacillus subtilis

ComEB

subtilis]

2640
ComE operon protein 1 [Bacillus
UniRef100_P39694

Bacillus subtilis

ComEA

subtilis]

2641
ComE operon protein 4 [Bacillus
UniRef100_P39696

Bacillus subtilis

ComER

subtilis]

2642
Hypothetical protein yqeM [Bacillus
UniRef100_P54458

Bacillus subtilis

YqeM

subtilis]

2643
Hypothetical protein yqeL [Bacillus
UniRef100_P54457

Bacillus subtilis

YqeL

subtilis]

2644
Hypothetical protein yqeK [Bacillus
UniRef100_P54456

Bacillus subtilis

YqeK

subtilis]

2645
Nicotinate-nucleotide
UniRef100_P54455

Bacillus subtilis

YqeJ

adenylyltransferase (EC 2.7.7.18)

(Deamido-NAD(+) pyrophosphorylase)

(Deamido-NAD(+) diphosphorylase)

[Bacillus subtilis]

2646
Hypothetical UPF0044 protein yqeI
UniRef100_P54454

Bacillus subtilis

[Bacillus subtilis]

2647
Shikimate dehydrogenase [Bacillus
UniRef100_P54374

Bacillus subtilis

AroD

subtilis]

2648
Hypothetical protein yqeH [Bacillus
UniRef100_P54453

Bacillus subtilis

YqeH

subtilis]

2649
Hypothetical protein yqeG [Bacillus
UniRef100_P54452

Bacillus subtilis

YqeG

subtilis]

2650

2651

2652
Hypothetical lipoprotein yqeF precursor
UniRef100_P54451

Bacillus subtilis

YqeF

[Bacillus subtilis]

2653
Acetyltransferase, GNAT family
UniRef100_Q81KW8

Bacillus

YdfB

[Bacillus anthracis]

anthracis

2654
Hypothetical protein yrhF [Bacillus
UniRef100_O05398

Bacillus subtilis

YrhF

subtilis]

2655
Formate dehydrogenase chain A
UniRef100_O05397

Bacillus subtilis

YrhE

[Bacillus subtilis]

2656
Hypothetical protein yrhD [Bacillus
UniRef100_O05396

Bacillus subtilis

YrhD

subtilis]

2657

2658
RNA polymerase sigma-K factor
UniRef100_P12254

Bacillus subtilis

SpoIIIC

precursor [Bacillus subtilis]

2659

YcnB

2660
BH2157 protein [Bacillus halodurans]
UniRef100_Q9KAX9

Bacillus

YuaI

halodurans

2661

2662

2663
Alanyl-tRNA synthetase family protein
UniRef100_Q81Y73

Bacillus

AlaS

[Bacillus anthracis]

anthracis

2664
METAL-ACTIVATED PYRIDOXAL
UniRef100_Q8YCI2

Brucella melitensis

ENZYME [Brucella melitensis]

2665
Probable translation initiation inhibitor
UniRef100_Q6LKM3

Photobacterium

YabJ

[Photobacterium profundum)]

profundum)

2666

YccC

2667
Putative threonine synthase
UniRef100_Q82IF6

Streptomyces

ThrC

[Streptomyces avermitilis]

avermitilis

2668

YabJ

2669
Hypothetical protein
UniRef100_Q8RBA0

Thermoanaerobacter

[Thermoanaerobacter tengcongensis]

tengcongensis

2670

2671
UPI000032CE59 UniRef100 entry
UniRef100_UPI000032CE59

2672
Multidrug-efflux transporter 1 regulator
UniRef100_P39075

Bacillus subtilis

BmrR

[Bacillus subtilis]

2673
Metallo-beta-lactamase/rhodanese-like
UniRef100_Q81Q95

Bacillus

YrkH

domain protein [Bacillus anthracis]

anthracis

2674
Hypothetical protein [Bacillus cereus
UniRef100_Q63B51

Bacillus cereus

YumB

ZK]

ZK

2675
NreC [Staphylococcus carnosus]
UniRef100_Q7WZY4

Staphylococcus

DegU

carnosus

2676
Two-component sensor histidine kinase
UniRef100_Q67JE7

Symbiobacterium

DegS

[Symbiobacterium thermophilum]

thermophilum

2677
YdfQ protein [Bacillus subtilis]
UniRef100_P96695

Bacillus subtilis

YdfQ

2678
Hypothetical Membrane Spanning
UniRef100_Q813Y5

Bacillus cereus

YrkJ

Protein [Bacillus cereus]

2679
Hypothetical UPF0033 protein yrkI
UniRef100_P54436

Bacillus subtilis

[Bacillus subtilis]

2680
UPI00003CB3C6 UniRef100 entry
UniRef100_UPI00003CB3C6

YrkH

2681
Molybdopterin biosynthesis MoeB
UniRef100_Q81HL2

Bacillus cereus

YrkF

protein [Bacillus cereus]

2682
Hypothetical protein yrkE [Bacillus
UniRef100_P54432

Bacillus subtilis

YrkE

subtilis]

2683
Hypothetical conserved protein
UniRef100_Q8EN37

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

2684
S-adenosylmethionine-dependent
UniRef100_Q97FB3

Clostridium

YcgJ

methyltransferase [Clostridium

acetobutylicum

acetobutylicum]

2685
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81N81

Bacillus anthracis

2686
YciB protein [Bacillus subtilis]
UniRef100_P94399

Bacillus subtilis

2687
Acetylxylan esterase related enzyme
UniRef100_Q97LM8

Clostridium acetobutylicum

[Clostridium acetobutylicum]

2688
Hypothetical UPF0161 protein
UniRef100_P61464

Bacillus cereus

BCE4947 [Bacillus cereus]

2689
Delta-aminolevulinic acid dehydratase
UniRef100_Q9K8G2

Bacillus halodurans

[Bacillus halodurans]

2690
6-phospho-3-hexuloisomerase [Bacillus
UniRef100_Q6TV53

Bacillus

HxlB

methanolicus]

methanolicus

2691
Probable hexulose-6-phosphate
UniRef100_P42405

Bacillus subtilis

HxlA

synthase [Bacillus subtilis]

2692
Transcriptional regulator [Bacillus
UniRef100_Q70KJ9

Bacillus

HxlR

amyloliquefaciens]

amyloliquefaciens

2693
Fatty acid desaturase [Bacillus subtilis]
UniRef100_O34653

Bacillus subtilis

Des

2694
Sensor kinase [Bacillus subtilis]
UniRef100_O34757

Bacillus subtilis

YocF

2695
Sensor regulator [Bacillus subtilis]
UniRef100_O34723

Bacillus subtilis

YocG

2696
UPI00003CC1E4 UniRef100 entry
UniRef100_UPI00003CC1E4

YcgT

2697
Nickel transport system [Bacillus
UniRef100_Q9KFB8

Bacillus

AppA

halodurans]

halodurans

2698
Nickel transport system [Bacillus
UniRef100_Q9KFB7

Bacillus

AppB

halodurans]

halodurans

2699
Nickel transport system [Bacillus
UniRef100_Q9KFB6

Bacillus

AppC

halodurans]

halodurans

2700
Oligopeptide ABC transporter [Bacillus
UniRef100_Q9KFB5

Bacillus

DppD

halodurans]

halodurans

2701
Oligopeptide ABC transporter [Bacillus
UniRef100_Q9KFB4

Bacillus

AppF

halodurans]

halodurans

2702
UPI00003CB880 UniRef100 entry
UniRef100_UPI00003CB880

YdfL

2703
UPI00003CA374 UniRef100 entry
UniRef100_UPI00003CA374

YoeA

2704
PROBABLE TRANSCRIPTION
UniRef100_Q8XS91

Ralstonia solanacearum

REGULATOR PROTEIN [Ralstonia

solanacearum]

2705
Short chain dehydrogenase family
UniRef100_Q834I5

Enterococcus

YvaG

protein [Enterococcus faecalis]

faecalis

2706
Uncharacterized protein, containing
UniRef100_Q97L24

Clostridium acetobutylicum

predicted phosphatase domain

[Clostridium acetobutylicum]

2707
UPI000025758C UniRef100 entry
UniRef100_UPI000025758C

PnbA

2708
Cytochrome P450 [Bacillus subtilis]
UniRef100_O08469

Bacillus subtilis

CypA

2709
YtnM [Bacillus subtilis]
UniRef100_O34430

Bacillus subtilis

YtnM

2710
Hypothetical protein yndA precursor
UniRef100_O31805

Bacillus subtilis

YndA

[Bacillus subtilis]

2711
YvaG protein [Bacillus subtilis]
UniRef100_O32229

Bacillus subtilis

YvaG

2712
Levanase precursor [Bacillus subtilis]
UniRef100_P05656

Bacillus subtilis

SacC

2713
PTS system, fructose-specific IID
UniRef100_P26382

Bacillus subtilis

LevG

component [Bacillus subtilis]

2714
PTS system, fructose-specific IIC
UniRef100_P26381

Bacillus subtilis

LevF

component [Bacillus subtilis]

2715
PTS system, fructose-specific IIB
UniRef100_P26380

Bacillus subtilis

LevE

component [Bacillus subtilis]

2716
PTS system, fructose-specific IIA
UniRef100_P26379

Bacillus subtilis

LevD

component [Bacillus subtilis]

2717
Transcriptional regulatory protein levR
UniRef100_P23914

Bacillus subtilis

LevR

[Bacillus subtilis]

2718
Hypothetical protein [Bacillus cereus]
UniRef100_Q734Q5

Bacillus cereus

2719
Hypothetical protein yrhK [Bacillus
UniRef100_O05401

Bacillus subtilis

subtilis]

2720
UPI00003CB785 UniRef100 entry
UniRef100_UPI00003CB785

2721

AdaA

2722
Methylated-DNA--protein-cysteine S-
UniRef100_Q732Y7

Bacillus cereus

AdaB

methyltransferase [Bacillus cereus]

2723
Oxidoreductase, aldo/keto reductase
UniRef100_Q6HBJ5

Bacillus

YtbE

family [Bacillus thuringiensis]

thuringiensis

2724

YtbD

2725
Hypothetical UPF0087 protein ytcD
UniRef100_O34533

Bacillus subtilis

YtcD

[Bacillus subtilis]

2726

2727
Hypothetical protein yjjA [Bacillus
UniRef100_O34394

Bacillus subtilis

YjjA

subtilis]

2728
UPI000028298B UniRef100 entry
UniRef100_UPI000028298B

2729

YxeB

2730
Putative HTH-type transcriptional
UniRef100_P37499

Bacillus subtilis

YybE

regulator yybE [Bacillus subtilis]

2731
Hypothetical transport protein yybF
UniRef100_P37498

Bacillus subtilis

YybF

[Bacillus subtilis]

2732
Hypothetical protein [Bacillus cereus]
UniRef100_Q732J0

Bacillus cereus

2733
Probable bifunctional P-450: NADPH-
UniRef100_O08336
Includes:
YrhJ

P450 reductase 2 [Includes:

Cytochrome

Cytochrome P450 102 (EC 1.14.14.1);

P450 102 (EC

NADPH--cytochrome P450 reductase

1.14.14.1);

(EC 1.6.2.4)] [Bacillus subtilis]

NADPH--

cytochrome

P450 reductase

(EC 1.6.2.4)

2734
Regulatory protein [Bacillus subtilis]
UniRef100_O08335

Bacillus subtilis

YrhI

2735

WprA

2736
YrhH [Bacillus subtilis]
UniRef100_O05400

Bacillus subtilis

YrhH

2737

2738

2739

2740
Cystathionine gamma-lyase [Bacillus
UniRef100_O05394

Bacillus subtilis

YrhB

subtilis]

2741
Cysteine synthase [Bacillus subtilis]
UniRef100_O05393

Bacillus subtilis

YrhA

2742
MTA/SAH nucleosidase [Bacillus
UniRef100_O32028

Bacillus subtilis

Mtn

subtilis]

2743
YrrT protein [Bacillus subtilis]
UniRef100_O32029

Bacillus subtilis

YrrT

2744
Hypothetical protein yrzA [Bacillus
UniRef100_O32030

Bacillus subtilis

subtilis]

2745

YrrS

2746
YrrR protein [Bacillus subtilis]
UniRef100_O32032

Bacillus subtilis

YrrR

2747
Transcription elongation factor greA
UniRef100_P80240

Bacillus subtilis

GreA

[Bacillus subtilis]

2748
Uridine kinase [Bacillus subtilis]
UniRef100_O32033

Bacillus subtilis

Udk

2749
YrrO protein [Bacillus subtilis]
UniRef100_O32034

Bacillus subtilis

YrrO

2750
YrrN protein [Bacillus subtilis]
UniRef100_O32035

Bacillus subtilis

YrrN

2751
YrrM protein [Bacillus subtilis]
UniRef100_O32036

Bacillus subtilis

YrrM

2752
YrrL protein [Bacillus subtilis]
UniRef100_O34758

Bacillus subtilis

YrrL

2753
YrzB protein [Bacillus subtilis]
UniRef100_O34828

Bacillus subtilis

2754
Putative Holliday junction resolvase
UniRef100_O34634

Bacillus subtilis

YrrK

[Bacillus subtilis]

2755
Hypothetical UPF0297 protein yrzL
UniRef100_Q7WY61

Bacillus subtilis

[Bacillus subtilis]

2756
Alanyl-tRNA synthetase [Bacillus
UniRef100_O34526

Bacillus subtilis

AlaS

subtilis]

2757
Hypothetical UPF0118 protein yrrI
UniRef100_O34472

Bacillus subtilis

YrrI

[Bacillus subtilis]

2758

2759
Hypothetical protein [Bacillus cereus
UniRef100_Q634F2

Bacillus cereus ZK

ZK]

2760
YrrD protein [Bacillus subtilis]
UniRef100_O34402

Bacillus subtilis

YrrD

2761
YrrC protein [Bacillus subtilis]
UniRef100_O34481

Bacillus subtilis

YrrC

2762
YrrB protein [Bacillus subtilis]
UniRef100_O34452

Bacillus subtilis

YrrB

2763
Probable tRNA (5-methylaminomethyl-
UniRef100_O35020

Bacillus subtilis

TrmU

2-thiouridylate)-methyltransferase

[Bacillus subtilis]

2764
YrvO protein [Bacillus subtilis]
UniRef100_O34599

Bacillus subtilis

YrvO

2765
BH1259 protein [Bacillus halodurans]
UniRef100_Q9KDF4

Bacillus halodurans

2766
YrvN protein [Bacillus subtilis]
UniRef100_O34528

Bacillus subtilis

YrvN

2767
YrvM protein [Bacillus subtilis]
UniRef100_O32037

Bacillus subtilis

YrvM

2768
Aspartyl-tRNA synthetase [Bacillus
UniRef100_O32038

Bacillus subtilis

AspS

subtilis]

2769
Histidyl-tRNA synthetase [Bacillus
UniRef100_O32039

Bacillus subtilis

HisS

subtilis]

2770

2771

YrvJ

2772
Putative D-tyrosyl-tRNA(Tyr)
UniRef100_O32042

Bacillus subtilis

YrvI

deacylase-like protein [Bacillus subtilis]

2773
GTP pyrophosphokinase (EC 2.7.6.5)
UniRef100_O54408

Bacillus subtilis

RelA

(ATP:GTP 3′-pyrophosphotransferase)

(ppGpp synthetase I) ((P)ppGpp

synthetase) [Bacillus subtilis]

2774
Adenine phosphoribosyltransferase
UniRef100_O34443

Bacillus subtilis

Apt

[Bacillus subtilis]

2775
YrvE protein [Bacillus subtilis]
UniRef100_O32044

Bacillus subtilis

YrvE

2776
YrvD protein [Bacillus subtilis]
UniRef100_O32045

Bacillus subtilis

2777
SecDF protein [Bacillus subtilis]
UniRef100_O32047

Bacillus subtilis

SecDF

2778
YrzD protein [Bacillus subtilis]
UniRef100_O32049

Bacillus subtilis

2779

SpoVB

2780
Hypothetical protein yrbG [Bacillus
UniRef100_O32050

Bacillus subtilis

YrbG

subtilis]

2781
YrzE protein [Bacillus subtilis]
UniRef100_O32051

Bacillus subtilis

YrzE

2782
Hypothetical UPF0092 protein yrbF
UniRef100_O32052

Bacillus subtilis

[Bacillus subtilis]

2783
Queuine tRNA-ribosyltransferase
UniRef100_O32053

Bacillus subtilis

Tgt

[Bacillus subtilis]

2784
S-adenosylmethionine:tRNA
UniRef100_O32054

Bacillus subtilis

QueA

ribosyltransferase-isomerase [Bacillus

subtilis]

2785

2786
Holliday junction DNA helicase ruvB
UniRef100_O32055

Bacillus subtilis

RuvB

[Bacillus subtilis]

2787
Holliday junction DNA helicase ruvA
UniRef100_O05392

Bacillus subtilis

RuvA

[Bacillus subtilis]

2788
BofC protein precursor [Bacillus subtilis]
UniRef100_O05391

Bacillus subtilis

BofC

2789
Hypothetical conserved protein
UniRef100_Q8ERL7

Oceanobacillus

YrzF

[Oceanobacillus iheyensis]

iheyensis

2790

2791
Small, acid-soluble spore protein H
UniRef100_Q9KB75

Bacillus halodurans

[Bacillus halodurans]

2792
Hypothetical protein yjoA [Bacillus
UniRef100_O34334

Bacillus subtilis

YjoA

subtilis]

2793
YmaC protein [Bacillus subtilis]
UniRef100_O31789

Bacillus subtilis

YmaC

2794

2795

2796
Hypothetical UPF0082 protein yrbC
UniRef100_P94447

Bacillus subtilis

YrbC

[Bacillus subtilis]

2797
Sporulation cortex protein coxA
UniRef100_P94446

Bacillus subtilis

CoxA

[Bacillus subtilis]

2798
Morphogenetic protein associated with
UniRef100_O32062

Bacillus subtilis

SafA

SpoVID [Bacillus subtilis]

2799
Quinolinate synthetase A [Bacillus
UniRef100_Q9KWZ1

Bacillus subtilis

NadA

subtilis]

2800
Probable nicotinate-nucleotide
UniRef100_P39666
carboxylating
NadC

pyrophosphorylase [carboxylating]

[Bacillus subtilis]

2801
L-aspartate oxidase [Bacillus subtilis]
UniRef100_P38032

Bacillus subtilis

NadB

2802
Probable cysteine desulfurase [Bacillus
UniRef100_P38033

Bacillus subtilis

NifS

subtilis]

2803

YrxA

2804
Prephenate dehydratase [Bacillus
UniRef100_P21203

Bacillus subtilis

PheA

subtilis]

2805
ACT domain protein pheB [Bacillus
UniRef100_P21204

Bacillus subtilis

PheB

subtilis]

2806
Spo0B-associated GTP-binding protein
UniRef100_Q659J4

Bacillus

Obg

[Bacillus amyloliquefaciens]

amyloliquefaciens

2807
Sporulation initiation
UniRef100_P06535

Bacillus subtilis

Spo0B

phosphotransferase B [Bacillus subtilis]

2808
50S ribosomal protein L27 [Bacillus
UniRef100_P05657

Bacillus subtilis

subtilis]

2809

2810
50S ribosomal protein L21 [Bacillus
UniRef100_P26908

Bacillus subtilis

RplU

subtilis]

2811
Stage IV sporulation protein FB
UniRef100_P26937

Bacillus subtilis

SpoIVFB

[Bacillus subtilis]

2812
Stage IV sporulation protein FA
UniRef100_P26936

Bacillus subtilis

SpoIVFA

[Bacillus subtilis]

2813
Hypothetical protein [Bacillus cereus]
UniRef100_Q816V6

Bacillus cereus

YndB

2814
Transcriptional regulator, ArsR family
UniRef100_Q632Y0

Bacillus cereus ZK

[Bacillus cereus ZK]

2815
Septum site-determining protein minD
UniRef100_Q01464

Bacillus subtilis

MinD

[Bacillus subtilis]

2816

MinC

2817
Rod shape-determining protein mreD
UniRef100_Q01467

Bacillus subtilis

MreD

[Bacillus subtilis]

2818
Rod shape-determining protein mreC
UniRef100_Q01466

Bacillus subtilis

MreC

[Bacillus subtilis]

2819
Rod shape-determining protein mreB
UniRef100_Q01465

Bacillus subtilis

MreB

[Bacillus subtilis]

2820
DNA repair protein radC homolog
UniRef100_Q02170

Bacillus subtilis

RadC

[Bacillus subtilis]

2821
Septum formation protein Maf [Bacillus
UniRef100_Q02169

Bacillus subtilis

Maf

subtilis]

2822
Stage II sporulation protein B [Bacillus
UniRef100_P37575

Bacillus subtilis

SpoIIB

subtilis]

2823
Type 4 prepilin-like proteins leader
UniRef100_P15378
Includes: Leader
ComC

peptide processing enzyme (Late

peptidase (EC

competence protein comC) [Includes:

3.4.23.43)

Leader peptidase (EC 3.4.23.43)

(Prepilin

(Prepilin peptidase); N-

peptidase); N-

methyltransferase (EC 2.1.1.—)] [Bacillus

methyltransferase

subtilis]

(EC 2.1.1.—)

2824

FolC

2825
Valyl-tRNA synthetase [Bacillus subtilis]
UniRef100_Q05873

Bacillus subtilis

ValS

2826
Hypothetical protein OB2062
UniRef100_Q8EPN1

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

2827
Hypothetical protein ysxE [Bacillus
UniRef100_P37964

Bacillus subtilis

YsxE

subtilis]

2828
Stage VI sporulation protein D [Bacillus
UniRef100_P37963

Bacillus subtilis

SpoVID

subtilis]

2829
Glutamate-1-semialdehyde 2,1-
UniRef100_P30949

Bacillus subtilis

HemL

aminomutase [Bacillus subtilis]

2830
Delta-aminolevulinic acid dehydratase
UniRef100_P30950

Bacillus subtilis

HemB

[Bacillus subtilis]

2831
Uroporphyrinogen-III synthase [Bacillus
UniRef100_P21248

Bacillus subtilis

HemD

subtilis]

2832
Porphobilinogen deaminase [Bacillus
UniRef100_P16616

Bacillus subtilis

HemC

subtilis]

2833
Protein hemX [Bacillus subtilis]
UniRef100_P16645

Bacillus subtilis

HemX

2834
Glutamyl-tRNA reductase [Bacillus
UniRef100_P16618

Bacillus subtilis

HemA

subtilis]

2835
Hypothetical protein ysxD [Bacillus
UniRef100_P40736

Bacillus subtilis

YsxD

subtilis]

2836
Probable GTP-binding protein engB
UniRef100_P38424

Bacillus subtilis

YsxC

[Bacillus subtilis]

2837
ATP-dependent protease La 1 [Bacillus
UniRef100_P37945

Bacillus subtilis

LonA

subtilis]

2838
ATP-dependent protease La homolog
UniRef100_P42425

Bacillus subtilis

LonB

[Bacillus subtilis]

2839
ATP-dependent Clp protease ATP-
UniRef100_P50866

Bacillus subtilis

ClpX

binding subunit clpX [Bacillus subtilis]

2840
Trigger factor [Bacillus subtilis]
UniRef100_P80698

Bacillus subtilis

Tig

2841
Hypothetical protein ysoA [Bacillus
UniRef100_P94569

Bacillus subtilis

YsoA

subtilis]

2842
3-isopropylmalate dehydratase small
UniRef100_P94568

Bacillus subtilis

LeuD

subunit [Bacillus subtilis]

2843
3-isopropylmalate dehydratase large
UniRef100_P80858

Bacillus subtilis

LeuC

subunit [Bacillus subtilis]

2844

LeuB

2845
2-isopropylmalate synthase [Bacillus
UniRef100_P94565

Bacillus subtilis

LeuA

subtilis]

2846
Ketol-acid reductoisomerase [Bacillus
UniRef100_P37253

Bacillus subtilis

IlvC

subtilis]

2847
Acetolactate synthase small subunit
UniRef100_P37252

Bacillus subtilis

IlvH

[Bacillus subtilis]

2848
Acetolactate synthase large subunit
UniRef100_P37251

Bacillus subtilis

IlvB

[Bacillus subtilis]

2849
Branched-chain amino acid
UniRef100_Q6HLF7

Bacillus

Dat

aminotransferase [Bacillus

thuringiensis

thuringiensis]

2850

2851

RocG

2852
BH3337 protein [Bacillus halodurans]
UniRef100_Q9K7M4

Bacillus halodurans

2853

YxeD

2854
Hypothetical protein yqbA [Bacillus
UniRef100_P45917

Bacillus subtilis

subtilis]

2855
Hypothetical protein yqaT [Bacillus
UniRef100_P45916

Bacillus subtilis

YqaT

subtilis]

2856
Lin1266 protein [Listeria innocua]
UniRef100_Q92CC3

Listeria innocua

2857
Lin1733 protein [Listeria innocua]
UniRef100_Q92B18

Listeria innocua

2858

2859

2860

2861

MtbP

2862
BH3535 protein [Bacillus halodurans]
UniRef100_Q9K738

Bacillus halodurans

2863

YeeF

2864

YxiD

2865

RapI

2866

2867

2868

2869
Hypothetical UPF0025 protein ysnB
UniRef100_P94559

Bacillus subtilis

YsnB

[Bacillus subtilis]

2870
HAM1 protein homolog [Bacillus
UniRef100_P94558

Bacillus subtilis

YsnA

subtilis]

2871

Rph

2872
Germination protein gerM [Bacillus
UniRef100_P39072

Bacillus subtilis

GerM

subtilis]

2873

RacE

2874
Hypothetical protein ysmB [Bacillus
UniRef100_P97247

Bacillus subtilis

YsmB

subtilis]

2875
Germination protein gerE [Bacillus
UniRef100_P11470

Bacillus subtilis

subtilis]

2876
Oxidoreductase [Clostridium
UniRef100_Q97TP7

Clostridium

YqjQ

acetobutylicum]

acetobutylicum

2877
Hypothetical protein ysmA [Bacillus
UniRef100_Q6L874

Bacillus subtilis

YsmA

subtilis]

2878
Succinate dehydrogenase iron-sulfur
UniRef100_P08066

Bacillus subtilis

SdhB

protein [Bacillus subtilis]

2879

SdhA

2880

2881
Succinate dehydrogenase cytochrome
UniRef100_P08064

Bacillus subtilis

SdhC

B-558 subunit [Bacillus subtilis]

2882
Hypothetical protein yslB [Bacillus
UniRef100_P42955

Bacillus subtilis

YslB

subtilis]

2883
Aspartokinase 2 (EC 2.7.2.4)
UniRef100_P08495
Contains:
LysC

(Aspartokinase II) (Aspartate kinase 2)

Aspartokinase II

[Contains: Aspartokinase II alpha

alpha subunit;

subunit; Aspartokinase II beta subunit]

Aspartokinase II

[Bacillus subtilis]

beta subunit

2884
UvrABC system protein C [Bacillus
UniRef100_P14951

Bacillus subtilis

UvrC

subtilis]

2885
Thioredoxin [Bacillus subtilis]
UniRef100_P14949

Bacillus subtilis

TrxA

2886
Electron transfer flavoprotein alpha-
UniRef100_P94551

Bacillus subtilis

EtfA

subunit [Bacillus subtilis]

2887
Electron transfer flavoprotein beta-
UniRef100_P94550

Bacillus subtilis

EtfB

subunit [Bacillus subtilis]

2888
Hypothetical protein ysiB [Bacillus
UniRef100_P94549

Bacillus subtilis

YsiB

subtilis]

2889
Hypothetical protein ysiA [Bacillus
UniRef100_P94548

Bacillus subtilis

YsiA

subtilis]

2890
Long-chain-fatty-acid--CoA ligase
UniRef100_P94547

Bacillus subtilis

LcfA

[Bacillus subtilis]

2891
Hypothetical protein yshE [Bacillus
UniRef100_P94546

Bacillus subtilis

YshE

subtilis]

2892
MutS2 protein [Bacillus subtilis]
UniRef100_P94545

Bacillus subtilis

MutSB

2893
Hypothetical protein yshC [Bacillus
UniRef100_P94544

Bacillus subtilis

YshC

subtilis]

2894

YshB

2895

2896
Ribonuclease HIII [Bacillus subtilis]
UniRef100_P94541

Bacillus subtilis

RnhC

2897

2898

2899

2900

2901

YxlF

2902

2903
Phenylalanyl-tRNA synthetase beta
UniRef100_P17922

Bacillus subtilis

PheT

chain [Bacillus subtilis]

2904
Phenylalanyl-tRNA synthetase alpha
UniRef100_Q659J3

Bacillus

PheS

chain [Bacillus amyloliquefaciens]

amyloliquefaciens

2905
Hypothetical protein ysgA [Bacillus
UniRef100_P94538

Bacillus subtilis

YsgA

subtilis]

2906
Small, acid-soluble spore protein I
UniRef100_P94537

Bacillus subtilis

[Bacillus subtilis]

2907
Carbon starvation protein A homolog
UniRef100_P94532

Bacillus subtilis

CstA

[Bacillus subtilis]

2908
Alpha-N-arabinofuranosidase [Bacillus
UniRef100_Q9XBQ3

Bacillus

AbfA

stearothermophilus]

stearothermophilus

2909
L-arabinose transport system permease
UniRef100_P94530

Bacillus subtilis

AraQ

protein araQ [Bacillus subtilis]

2910
L-arabinose transport system permease
UniRef100_P94529

Bacillus subtilis

AraP

protein araP [Bacillus subtilis]

2911
Probable arabinose-binding protein
UniRef100_P94528

Bacillus subtilis

AraN

precursor [Bacillus subtilis]

2912
Arabinose operon protein araM
UniRef100_P94527

Bacillus subtilis

AraM

[Bacillus subtilis]

2913
L-ribulose-5-phosphate 4-epimerase
UniRef100_P94525

Bacillus subtilis

AraD

[Bacillus subtilis]

2914
Ribulokinase [Bacillus subtilis]
UniRef100_P94524

Bacillus subtilis

AraB

2915
L-arabinose isomerase [Bacillus
UniRef100_P94523

Bacillus subtilis

AraA

subtilis]

2916

AbnA

2917
Hypothetical protein ysdC [Bacillus
UniRef100_P94521

Bacillus subtilis

YsdC

subtilis]

2918
Hypothetical protein ysdB [Bacillus
UniRef100_P94520

Bacillus subtilis

YsdB

subtilis]

2919
Hypothetical protein ysdA [Bacillus
UniRef100_P94519

Bacillus subtilis

subtilis]

2920
50S ribosomal protein L20 [Bacillus
UniRef100_P55873

Bacillus subtilis

RplT

subtilis]

2921
50S ribosomal protein L35 [Bacillus
UniRef100_P55874

Bacillus subtilis

subtilis]

2922

InfC

2923
Antiholin-like protein IrgB [Bacillus
UniRef100_P94516

Bacillus subtilis

YsbB

subtilis]

2924
Antiholin-like protein IrgA [Bacillus
UniRef100_P94515

Bacillus subtilis

YsbA

subtilis]

2925

PhoD

2926
Sensory transduction protein lytT
UniRef100_P94514

Bacillus subtilis

LytT

[Bacillus subtilis]

2927
Sensor protein lytS [Bacillus subtilis]
UniRef100_P94513

Bacillus subtilis

LytS

2928
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81N00

Bacillus anthracis

2929
Hypothetical protein ysaA [Bacillus
UniRef100_P94512

Bacillus subtilis

YsaA

subtilis]

2930
Threonyl-tRNA synthetase 1 [Bacillus
UniRef100_P18255

Bacillus subtilis

ThrS

subtilis]

2931
Hypothetical protein ytxC [Bacillus
UniRef100_P06569

Bacillus subtilis

YtxC

subtilis]

2932
Hypothetical UPF0043 protein ytxB
UniRef100_P06568

Bacillus subtilis

YtxB

[Bacillus subtilis]

2933
Primosomal protein dnaI [Bacillus
UniRef100_P06567

Bacillus subtilis

DnaI

subtilis]

2934
Replication initiation and membrane
UniRef100_P07908

Bacillus subtilis

DnaB

attachment protein [Bacillus subtilis]

2935
Hypothetical UPF0168 protein ytcG
UniRef100_Q45549

Bacillus subtilis

YtcG

[Bacillus subtilis]

2936

2937

SpeD

2938

GapB

2939
Pectin lyase [Bacillus subtilis]
UniRef100_P94449

Bacillus subtilis

PelB

2940
Dephospho-CoA kinase [Bacillus
UniRef100_O34932

Bacillus subtilis

YtaG

subtilis]

2941

YtaF

2942
Formamidopyrimidine-DNA glycosylase
UniRef100_O34403

Bacillus subtilis

MutM

[Bacillus subtilis]

2943
DNA polymerase I [Bacillus subtilis]
UniRef100_O34996

Bacillus subtilis

PolA

2944
Alkaline phosphatase synthesis sensor
UniRef100_P23545

Bacillus subtilis

PhoR

protein phoR [Bacillus subtilis]

2945
Alkaline phosphatase synthesis
UniRef100_P13792

Bacillus subtilis

PhoP

transcriptional regulatory protein phoP

[Bacillus subtilis]

2946
Malate dehydrogenase [Bacillus
UniRef100_P49814

Bacillus subtilis

Mdh

subtilis]

2947
Isocitrate dehydrogenase [NADP]
UniRef100_P39126
NADP
Icd

[Bacillus subtilis]

2948
Citrate synthase II [Bacillus subtilis]
UniRef100_P39120

Bacillus subtilis

CitZ

2949
YtwI [Bacillus subtilis]
UniRef100_O34811

Bacillus subtilis

YtwI

2950
Hypothetical UPF0118 protein ytvI
UniRef100_O34991

Bacillus subtilis

YtvI

[Bacillus subtilis]

2951
YtzA protein [Bacillus subtilis]
UniRef100_O32064

Bacillus subtilis

YtzA

2952

Pyk

2953
6-phosphofructokinase [Bacillus
UniRef100_O34529

Bacillus subtilis

PfkA

subtilis]

2954
Acetyl-coenzyme A carboxylase
UniRef100_O34847

Bacillus subtilis

AccA

carboxyl transferase subunit alpha

[Bacillus subtilis]

2955
Acetyl-CoA carboxylase subunit
UniRef100_O34571

Bacillus subtilis

AccD

[Bacillus subtilis]

2956

YtsJ

2957
DNA polymerase III alpha subunit
UniRef100_O34623

Bacillus subtilis

DnaE

[Bacillus subtilis]

2958
Hypothetical Membrane Spanning
UniRef100_Q812P3

Bacillus cereus

Protein [Bacillus cereus]

2959
YtrI [Bacillus subtilis]
UniRef100_O34460

Bacillus subtilis

YtrI

2960
BH3172 protein [Bacillus halodurans]
UniRef100_Q9K835

Bacillus halodurans

2961
YtqI [Bacillus subtilis]
UniRef100_O34600

Bacillus subtilis

YtqI

2962
YtpI [Bacillus subtilis]
UniRef100_O34922

Bacillus subtilis

2963

YtoI

2964

PadR

2965
YtkL protein [Bacillus subtilis]
UniRef100_Q795U4

Bacillus subtilis

YtkL

2966

YtkK

2967

2968
Argininosuccinate lyase [Bacillus
UniRef100_O34858

Bacillus subtilis

ArgH

subtilis]

2969
Argininosuccinate synthase [Bacillus
UniRef100_O34347

Bacillus subtilis

ArgG

subtilis]

2970
Molybdenum cofactor biosynthesis
UniRef100_O34457

Bacillus subtilis

MoaB

protein B [Bacillus subtilis]

2971

AckA

2972
Hypothetical protein ytxK [Bacillus
UniRef100_P37876

Bacillus subtilis

YtxK

subtilis]

2973
Probable thiol peroxidase [Bacillus
UniRef100_P80864

Bacillus subtilis

Tpx

subtilis]

2974
YtfJ [Bacillus subtilis]
UniRef100_O34806

Bacillus subtilis

YtfJ

2975

YtfI

2976
YteJ [Bacillus subtilis]
UniRef100_O34424

Bacillus subtilis

YteJ

2977
Putative signal peptide peptidase sppA
UniRef100_O34525

Bacillus subtilis

SppA

[Bacillus subtilis]

2978
Probable inorganic polyphosphate/ATP-
UniRef100_O34934

Bacillus subtilis

YtdI

NAD kinase 2 (EC 2.7.1.23)

(Poly(P)/ATP NAD kinase 2) [Bacillus

subtilis]

2979
YhbJ protein [Bacillus subtilis]
UniRef100_O31593

Bacillus subtilis

YhbJ

2980
Multidrug resistance protein
UniRef100_Q8CQB1

Staphylococcus

YubD

[Staphylococcus epidermidis]

epidermidis

2981
Putative HTH-type transcriptional
UniRef100_P42103

Bacillus subtilis

YxaD

regulator yxaD [Bacillus subtilis]

2982
YtcI [Bacillus subtilis]
UniRef100_O34613

Bacillus subtilis

YtcI

2983
Small, acid-soluble spore protein 1
UniRef100_P06552

Bacillus stearothermophilus

[Bacillus stearothermophilus]

2984
Probable thiamine biosynthesis protein
UniRef100_O34595

Bacillus subtilis

YtbJ

thil [Bacillus subtilis]

2985
NifS2 [Bacillus subtilis]
UniRef100_O34874

Bacillus subtilis

NifZ

2986
Branched-chain amino acid transport
UniRef100_O34545

Bacillus subtilis

BraB

system carrier protein braB [Bacillus

subtilis]

2987
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012

anthracis str. A2012]

2988
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069

2989
Septation ring formation regulator ezrA
UniRef100_O34894

Bacillus subtilis

EzrA

[Bacillus subtilis]

2990
Histidinol-phosphatase [Bacillus
UniRef100_O34411

Bacillus subtilis

HisJ

subtilis]

2991
Probable HTH-type transcriptional
UniRef100_O34970

Bacillus subtilis

YttP

regulator yttP [Bacillus subtilis]

2992
Hypothetical conserved protein
UniRef100_Q8EPB0

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

2993
YtrP [Bacillus subtilis]
UniRef100_O34325

Bacillus subtilis

YtrP

2994
30S ribosomal protein S4 [Bacillus
UniRef100_P21466

Bacillus subtilis

RpsD

subtilis]

2995

2996

2997

YddR

2998
HTH-type transcriptional regulator lrpA
UniRef100_P96652

Bacillus subtilis

LrpA

[Bacillus subtilis]

2999

3000
Tyrosyl-tRNA synthetase 1 [Bacillus
UniRef100_P22326

Bacillus subtilis

TyrS

subtilis]

3001
Acetyl-coenzyme A synthetase [Bacillus
UniRef100_P39062

Bacillus subtilis

AcsA

subtilis]

3002
Acetoin utilization protein acuA [Bacillus
UniRef100_P39065

Bacillus subtilis

AcuA

subtilis]

3003
Acetoin utilization acuB protein [Bacillus
UniRef100_P39066

Bacillus subtilis

AcuB

subtilis]

3004
Acetoin utilization protein acuC
UniRef100_P39067

Bacillus subtilis

AcuC

[Bacillus subtilis]

3005
Hypothetical protein ytxE [Bacillus
UniRef100_P39064

Bacillus subtilis

YtxE

subtilis]

3006
Hypothetical protein ytxD [Bacillus
UniRef100_P39063

Bacillus subtilis

YtxD

subtilis]

3007
Catabolite control protein A [Bacillus
UniRef100_P25144

Bacillus subtilis

CcpA

subtilis]

3008
AroA(G) protein [Includes: Phospho-2-
UniRef100_P39912
Includes:
AroA

dehydro-3-deoxyheptonate aldolase

Phospho-2-

(EC 2.5.1.54) (Phospho-2-keto-3-

dehydro-3-

deoxyheptonate aldolase) (DAHP

deoxyheptonate

synthetase) (3-deoxy-D-arabino-

aldolase (EC

heptulosonate 7-phosphate synthase);

2.5.1.54)

Chorismate mutase (EC 5.4.99.5)]

(Phospho-2-keto-

[Bacillus subtilis]

3-

deoxyheptonate

aldolase) (DAHP

synthetase) (3-

deoxy-D-arabino-

heptulosonate 7-

phosphate

synthase);

Chorismate

mutase (EC

5.4.99.5)

3009
Similar to hypothetical repeat
UniRef100_Q7N3B8

Photorhabdus luminescens

containing protein [Photorhabdus

luminescens]

3010
Hypothetical protein ytxJ [Bacillus
UniRef100_P39914

Bacillus subtilis

YtxJ

subtilis]

3011
Hypothetical protein ytxH [Bacillus
UniRef100_P40780

Bacillus subtilis

YtxH

subtilis]

3012
Hypothetical protein ytxG [Bacillus
UniRef100_P40779

Bacillus subtilis

YtxG

subtilis]

3013

MurC

3014
YtpT [Bacillus subtilis]
UniRef100_O34749

Bacillus subtilis

YtpT

3015
YtpR [Bacillus subtilis]
UniRef100_O34943

Bacillus subtilis

YtpR

3016
YtpQ [Bacillus subtilis]
UniRef100_O34496

Bacillus subtilis

YtpQ

3017
Putative thioredoxin [Bacillus subtilis]
UniRef100_O34357

Bacillus subtilis

YtpP

3018
YtoQ [Bacillus subtilis]
UniRef100_O34305

Bacillus subtilis

YtoQ

3019
YtoP [Bacillus subtilis]
UniRef100_O34924

Bacillus subtilis

YtoP

3020
YtzB protein [Bacillus subtilis]
UniRef100_O32065

Bacillus subtilis

YtzB

3021
Probable NAD-dependent malic
UniRef100_O34389

Bacillus subtilis

MalS

enzyme 3 [Bacillus subtilis]

3022
YtnP [Bacillus subtilis]
UniRef100_O34760

Bacillus subtilis

YtnP

3023
tRNA (guanine-N(7)-)-
UniRef100_O34522

Bacillus subtilis

YtmQ

methyltransferase (EC 2.1.1.33)

(tRNA(m7G46)-methyltransferase)

[Bacillus subtilis]

3024
YtzH protein [Bacillus subtilis]
UniRef100_O32066

Bacillus subtilis

3025
YtmP [Bacillus subtilis]
UniRef100_O34935

Bacillus subtilis

YtmP

3026
AmyX protein [Bacillus subtilis]
UniRef100_O34587

Bacillus subtilis

AmyX

3027
YtlR [Bacillus subtilis]
UniRef100_O34799

Bacillus subtilis

YtlR

3028
YtlQ [Bacillus subtilis]
UniRef100_O34471

Bacillus subtilis

YtlQ

3029
Hypothetical UPF0097 protein ytlP
UniRef100_O34570

Bacillus subtilis

YtlP

[Bacillus subtilis]

3030
Probable cysteine synthase (EC
UniRef100_O34476

Bacillus subtilis

YtkP

2.5.1.47) (O-acetylserine sulfhydrylase)

(O-acetylserine (Thiol)-lyase) [Bacillus

subtilis]

3031
Hypothetical protein [Bacillus cereus]
UniRef100_Q81BR8

Bacillus cereus

YncE

3032
Putative peptidase [Bacillus subtilis]
UniRef100_O34944

Bacillus subtilis

YtjP

3033
YtiP [Bacillus subtilis]
UniRef100_O34978

Bacillus subtilis

YtiP

3034
YtzE protein [Bacillus subtilis]
UniRef100_O32067

Bacillus subtilis

3035
Ribosomal small subunit pseudouridine
UniRef100_Q816W1

Bacillus cereus

YtzF

synthase A [Bacillus cereus]

3036
YtgP [Bacillus subtilis]
UniRef100_O34674

Bacillus subtilis

YtgP

3037
YtfP [Bacillus subtilis]
UniRef100_O30505

Bacillus subtilis

YtfP

3038

OpuD

3039
Protein cse60 [Bacillus subtilis]
UniRef100_P94496

Bacillus subtilis

3040
Rhodanese-like domain protein
UniRef100_Q72YZ9

Bacillus cereus

[Bacillus cereus]

3041

RapA

3042
Hypothetical protein [Bacillus
UniRef100_Q6HI31

Bacillus thuringiensis

thuringiensis]

3043
YteU [Bacillus subtilis]
UniRef100_O34378

Bacillus subtilis

YteU

3044

YteT

3045

YteS

3046
YteR [Bacillus subtilis]
UniRef100_O34559

Bacillus subtilis

YteR

3047
Transmembrane lipoprotein [Bacillus
UniRef100_Q9KFJ5

Bacillus

LplB

halodurans]

halodurans

3048
YtdP protein [Bacillus subtilis]
UniRef100_O32071

Bacillus subtilis

YtdP

3049
YtcQ protein [Bacillus subtilis]
UniRef100_Q795R2

Bacillus subtilis

YtcQ

3050

YtcP

3051
Hypothetical protein ytbQ [Bacillus
UniRef100_P53560

Bacillus subtilis

YtbQ

subtilis]

3052
YtaP [Bacillus subtilis]
UniRef100_O34973

Bacillus subtilis

YtaP

3053
Amino acid/polyamine transporter;
UniRef100_Q6LYX9

Methanococcus

YecA

family I [Methanococcus maripaludis]

maripaludis

3054
Transcriptional regulator, LysR family
UniRef100_Q97DX1

Clostridium

YwqM

[Clostridium acetobutylicum]

acetobutylicum

3055
Prolyl endopeptidase [Bacillus cereus]
UniRef100_Q81C54

Bacillus cereus

YycE

3056
Leucyl-tRNA synthetase [Bacillus
UniRef100_P36430

Bacillus subtilis

LeuS

subtilis]

3057

YtvB

3058
YttB [Bacillus subtilis]
UniRef100_O34546

Bacillus subtilis

YttB

3059
Lipoprotein [Oceanobacillus iheyensis]
UniRef100_Q8EPK3

Oceanobacillus

YusA

iheyensis

3060
YttA [Bacillus subtilis]
UniRef100_O30500

Bacillus subtilis

YttA

3061
YtrF [Bacillus subtilis]
UniRef100_O35005

Bacillus subtilis

YtrF

3062
Hypothetical ABC transporter ATP-
UniRef100_O34392

Bacillus subtilis

YtrE

binding protein ytrE [Bacillus subtilis]

3063
YtrC [Bacillus subtilis]
UniRef100_O34898

Bacillus subtilis

YtrC

3064
Transporter [Bacillus subtilis]
UniRef100_O34641

Bacillus subtilis

YtrB

3065
Transcription regulator [Bacillus subtilis]
UniRef100_O34712

Bacillus subtilis

YtrA

3066
Hypothetical protein ytzC [Bacillus
UniRef100_O32073

Bacillus subtilis

subtilis]

3067
YtqA [Bacillus subtilis]
UniRef100_O35008

Bacillus subtilis

YtqA

3068

YtqB

3069
Proton glutamate symport protein
UniRef100_P39817

Bacillus subtilis

GltP

[Bacillus subtilis]

3070
Hypothetical protein ytpB [Bacillus
UniRef100_O34707

Bacillus subtilis

YtpB

subtilis]

3071
Probable lysophospholipase [Bacillus
UniRef100_O34705

Bacillus subtilis

YtpA

subtilis]

3072
YtoA [Bacillus subtilis]
UniRef100_O34696

Bacillus subtilis

YtoA

3073

YwoA

3074
Glycosyl transferase, group 1 family
UniRef100_Q6HCB9

Bacillus

TuaC

[Bacillus thuringiensis]

thuringiensis

3075
Asparagine synthetase [glutamine-
UniRef100_P54420
glutamine-
AsnB

hydrolyzing] 1 [Bacillus subtilis]

hydrolyzing

3076
S-adenosylmethionine synthetase
UniRef100_P54419

Bacillus subtilis

MetK

[Bacillus subtilis]

3077
Phosphoenolpyruvate carboxykinase
UniRef100_P54418
ATP
PckA

[ATP] [Bacillus subtilis]

3078
Sodium:dicarboxylate symporter
UniRef100_Q8EP16

Oceanobacillus

DctP

[Oceanobacillus iheyensis]

iheyensis

3079
Hypothetical protein ytmB [Bacillus
UniRef100_O34365

Bacillus subtilis

subtilis]

3080
Putative peptidase [Bacillus subtilis]
UniRef100_O34493

Bacillus subtilis

YtmA

3081
ABC transporter substrate-binding
UniRef100_Q816P5

Bacillus cereus

YtlA

protein [Bacillus cereus]

3082
Putative transporter [Bacillus subtilis]
UniRef100_O34314

Bacillus subtilis

YtlC

3083

YtlD

3084
YtkD [Bacillus subtilis]
UniRef100_O35013

Bacillus subtilis

YtkD

3085
Hypothetical protein [Bacillus
UniRef100_Q6HC91

Bacillus thuringiensis

thuringiensis]

3086
Hypothetical protein ytkC [Bacillus
UniRef100_O34883

Bacillus subtilis

YtkC

subtilis]

3087
General stress protein 20U [Bacillus
UniRef100_P80879

Bacillus subtilis

Dps

subtilis]

3088
Hypothetical protein ytkA [Bacillus
UniRef100_P40768

Bacillus subtilis

YtkA

subtilis]

3089
S-ribosylhomocysteinase [Bacillus
UniRef100_O34667

Bacillus subtilis

LuxS

subtilis]

3090
Hypothetical UPF0161 protein ytjA
UniRef100_O34601

Bacillus subtilis

[Bacillus subtilis]

3091
YtiB [Bacillus subtilis]
UniRef100_O34872

Bacillus subtilis

YtiB

3092
Low-affinity zinc transport protein
UniRef100_Q81F90

Bacillus cereus

YciC

[Bacillus cereus]

3093
High-affinity zinc uptake system protein
UniRef100_Q81EF8

Bacillus cereus

YcdH

znuA [Bacillus cereus]

3094
50S ribosomal protein L31 type B
UniRef100_O34967

Bacillus subtilis

[Bacillus subtilis]

3095
YthA [Bacillus subtilis]
UniRef100_O34655

Bacillus subtilis

YthA

3096
YthB [Bacillus subtilis]
UniRef100_O34505

Bacillus subtilis

YthB

3097
Hypothetical protein [Bacillus cereus]
UniRef100_Q737J1

Bacillus cereus

3098

3099
O-succinylbenzoate synthase (EC
UniRef100_O34514

Bacillus subtilis

MenC

4.2.1.—) (OSB synthase) (OSBS) (4-(2′-

carboxyphenyl)-4-oxybutyric acid

synthase) [Bacillus subtilis]

3100
O-succinylbenzoate--CoA ligase
UniRef100_P23971

Bacillus subtilis

MenE

[Bacillus subtilis]

3101
Naphthoate synthase [Bacillus subtilis]
UniRef100_P23966

Bacillus subtilis

MenB

3102

YtxM

3103
Menaquinone biosynthesis protein
UniRef100_P23970
Includes: 2-
MenD

menD [Includes: 2-succinyl-6-hydroxy-

succinyl-6-

2,4-cyclohexadiene-1-carboxylate

hydroxy-2,4-

synthase (EC 2.5.1.64) (SHCHC

cyclohexadiene-

synthase); 2-oxoglutarate

1-carboxylate

decarboxylase (EC 4.1.1.71) (Alpha-

synthase (EC

ketoglutarate decarboxylase) (KDC)]

2.5.1.64)

[Bacillus subtilis]

(SHCHC

synthase); 2-

oxoglutarate

decarboxylase

(EC 4.1.1.71)

(Alpha-

ketoglutarate

decarboxylase)

(KDC)

3104
Menaquinone-specific isochorismate
UniRef100_P23973

Bacillus subtilis

MenF

synthase [Bacillus subtilis]

3105
Probable 1,4-dihydroxy-2-naphthoate
UniRef100_P39582

Bacillus subtilis

MenA

octaprenyltransferase [Bacillus subtilis]

3106
Hypothetical protein yteA [Bacillus
UniRef100_P42408

Bacillus subtilis

YteA

subtilis]

3107
Glycogen phosphorylase [Bacillus
UniRef100_P39123

Bacillus subtilis

GlgP

subtilis]

3108
Glycogen synthase [Bacillus subtilis]
UniRef100_P39125

Bacillus subtilis

GlgA

3109
Glycogen biosynthesis protein glgD
UniRef100_P39124

Bacillus subtilis

GlgD

[Bacillus subtilis]

3110
Glucose-1-phosphate
UniRef100_P39122

Bacillus subtilis

GlgC

adenylyltransferase [Bacillus subtilis]

3111
1,4-alpha-glucan branching enzyme
UniRef100_P39118

Bacillus subtilis

GlgB

[Bacillus subtilis]

3112

AraR

3113
YuaJ protein [Bacillus subtilis]
UniRef100_O32074

Bacillus subtilis

YuaJ

3114
BH4010 protein [Bacillus halodurans]
UniRef100_Q9K5S8

Bacillus

YhcS

halodurans

3115
BH4011 protein [Bacillus halodurans]
UniRef100_Q9K5S7

Bacillus halodurans

3116

3117

RapD

3118
Pyrrolidone-carboxylate peptidase
UniRef100_P46107

Bacillus

Pcp

[Bacillus amyloliquefaciens]

amyloliquefaciens

3119
BH0597 protein [Bacillus halodurans]
UniRef100_Q9KF88

Bacillus

YuaA

halodurans

3120

YubG

3121

YxxF

3122
YuaE protein [Bacillus subtilis]
UniRef100_O32078

Bacillus subtilis

YuaE

3123
YuaD protein [Bacillus subtilis]
UniRef100_O32079

Bacillus subtilis

YuaD

3124
Alcohol dehydrogenase [Bacillus
UniRef100_P71017

Bacillus subtilis

GbsB

subtilis]

3125
Betaine aldehyde dehydrogenase
UniRef100_P71016

Bacillus subtilis

GbsA

[Bacillus subtilis]

3126
Hypothetical protein yuaC [Bacillus
UniRef100_P71015

Bacillus subtilis

YuaC

subtilis]

3127
UPI00002D3D35 UniRef100 entry
UniRef100_UPI00002D3D35

OpuE

3128
Hypothetical protein yktD [Bacillus
UniRef100_Q45500

Bacillus subtilis

YktD

subtilis]

3129
Alanine racemase 2 [Bacillus subtilis]
UniRef100_P94494

Bacillus subtilis

YncD

3130
Oxalate decarboxylase [Bacillus
UniRef100_Q81GZ6

Bacillus cereus

YoaN

cereus]

3131
Hypothetical protein CAC0135
UniRef100_Q97MQ7

Clostridium acetobutylicum

[Clostridium acetobutylicum]

3132
Hypothetical protein [Bacillus
UniRef100_Q6HGC9

Bacillus thuringiensis

thuringiensis]

3133
Hypothetical protein [Bacillus
UniRef100_Q6HGC8

Bacillus thuringiensis

thuringiensis]

3134

3135
Hypothetical protein [Bacillus
UniRef100_Q6HGC6

Bacillus thuringiensis

thuringiensis]

3136
Hypothetical protein [Bacillus
UniRef100_Q6HGC5

Bacillus thuringiensis

thuringiensis]

3137
Hypothetical conserved protein
UniRef100_Q8ETF5

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

3138

3139

3140
YkoN [Bacillus subtilis]
UniRef100_O34625

Bacillus subtilis

YkoN

3141
Hypothetical protein ykoP [Bacillus
UniRef100_O34495

Bacillus subtilis

YkoP

subtilis]

3142
Hypothetical UPF0151 protein ykoQ
UniRef100_O35040

Bacillus subtilis

YkoQ

[Bacillus subtilis]

3143
Undecaprenyl-diphosphatase [Bacillus
UniRef100_P94507

Bacillus subtilis

YubB

subtilis]

3144
Hypothetical UPF0118 protein yubA
UniRef100_O32086

Bacillus subtilis

YubA

[Bacillus subtilis]

3145
Hypothetical oxidoreductase yulF
UniRef100_O05265

Bacillus subtilis

YulF

[Bacillus subtilis]

3146
Lmo2256 protein [Listeria
UniRef100_Q929B9

Listeria

YraA

monocytogenes]

monocytogenes

3147

McpA

3148

McpA

3149

McpA

3150
Protein-glutamine gamma-
UniRef100_P40746

Bacillus subtilis

Tgl

glutamyltransferase [Bacillus subtilis]

3151
2-nitropropane dioxygenase [Bacillus
UniRef100_O05413

Bacillus subtilis

YrpB

subtilis]

3152
Hypothetical UPF0047 protein yugU
UniRef100_O05243

Bacillus subtilis

YugU

[Bacillus subtilis]

3153
Hypothetical protein yugT [Bacillus
UniRef100_O05242

Bacillus subtilis

YugT

subtilis]

3154
Transcriptional regulator, TetR family
UniRef100_Q81GX6

Bacillus cereus

YfiR

[Bacillus cereus]

3155
Hypothetical protein yqeB [Bacillus
UniRef100_P54447

Bacillus subtilis

YqeB

subtilis]

3156
Beta(1,4)-glucan glucanohydrolase
UniRef100_Q6D3B7

Erwinia carotovora

[Erwinia carotovora]

3157
Hypothetical UPF0053 protein yugS
UniRef100_O05241

Bacillus subtilis

YugS

[Bacillus subtilis]

3158
Hypothetical protein yugP [Bacillus
UniRef100_O05248

Bacillus subtilis

YugP

subtilis]

3159
YugO protein [Bacillus subtilis]
UniRef100_Q795M8

Bacillus subtilis

3160
Hypothetical protein yugN [Bacillus
UniRef100_O05246

Bacillus subtilis

YugN

subtilis]

3161
Hypothetical protein [Bacillus
UniRef100_Q6HIW1

Bacillus

YdfR

thuringiensis]

thuringiensis

3162
YtaB protein [Bacillus subtilis]
UniRef100_O34694

Bacillus subtilis

YtaB

3163
Predicted acetyltransferase [Clostridium
UniRef100_Q97IT3

Clostridium

YkkB

acetobutylicum]

acetobutylicum

3164
Glucose-6-phosphate isomerase
UniRef100_P80860

Bacillus subtilis

Pgi

[Bacillus subtilis]

3165
Probable NADH-dependent butanol
UniRef100_O05239

Bacillus subtilis

YugJ

dehydrogenase 1 [Bacillus subtilis]

3166
YuzA protein [Bacillus subtilis]
UniRef100_O32087

Bacillus subtilis

3167
General stress protein 13 [Bacillus
UniRef100_P80870

Bacillus subtilis

YugI

subtilis]

3168
Alanine transaminase [Bacillus subtilis]
UniRef100_Q795M6

Bacillus subtilis

AlaT

3169
Transcriptional regulator [Bacillus
UniRef100_O05236

Bacillus subtilis

AlaR

subtilis]

3170
Hypothetical protein yugF [Bacillus
UniRef100_O05235

Bacillus subtilis

YugF

subtilis]

3171
Hypothetical protein yugE [Bacillus
UniRef100_O05234

Bacillus subtilis

subtilis]

3172
Hypothetical protein SMU.305
UniRef100_Q9X669

Streptococcus mutans

[Streptococcus mutans]

3173
Putative aminotransferase B [Bacillus
UniRef100_Q08432

Bacillus subtilis

PatB

subtilis]

3174

3175
Kinase-associated lipoprotein B
UniRef100_Q08429

Bacillus subtilis

KapB

precursor [Bacillus subtilis]

3176
Hypothetical protein yugB [Bacillus
UniRef100_O05231

Bacillus subtilis

KapD

subtilis]

3177

YuxJ

3178

PbpD

3179
Hypothetical protein yuxK [Bacillus
UniRef100_P40761

Bacillus subtilis

YuxK

subtilis]

3180
Hypothetical protein yufK [Bacillus
UniRef100_O05249

Bacillus subtilis

YufK

subtilis]

3181
Hypothetical protein yufL [Bacillus
UniRef100_O05250

Bacillus subtilis

YufL

subtilis]

3182
Hypothetical protein yufM [Bacillus
UniRef100_O05251

Bacillus subtilis

YufM

subtilis]

3183

3184
UPI00003CB938 UniRef100 entry
UniRef100_UPI00003CB938

PssA

3185
Hypothetical protein ybfM [Bacillus
UniRef100_O31453

Bacillus subtilis

YbfM

subtilis]

3186
Phosphatidylserine decarboxylase
UniRef100_Q6HDI5

Bacillus

Psd

[Bacillus thuringiensis]

thuringiensis

3187
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069

3188
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012

anthracis str. A2012]

3189
Na(+)-malate symporter [Bacillus
UniRef100_O05256

Bacillus subtilis

MaeN

subtilis]

3190

3191
Na(+)/H(+) antiporter subunit A
UniRef100_Q9K2S2

Bacillus subtilis

MrpA

[Bacillus subtilis]

3192
Na(+)/H(+) antiporter subunit B
UniRef100_O05259

Bacillus subtilis

MrpB

[Bacillus subtilis]

3193
Na(+)/H(+) antiporter subunit C
UniRef100_O05260

Bacillus subtilis

MrpC

[Bacillus subtilis]

3194
Na(+)/H(+) antiporter subunit D
UniRef100_O05229

Bacillus subtilis

MrpD

[Bacillus subtilis]

3195
Na(+)/H(+) antiporter subunit E
UniRef100_Q7WY60

Bacillus subtilis

MrpE

[Bacillus subtilis]

3196
Na(+)/H(+) antiporter subunit F
UniRef100_O05228

Bacillus subtilis

[Bacillus subtilis]

3197
Na(+)/H(+) antiporter subunit G
UniRef100_O05227

Bacillus subtilis

MrpG

[Bacillus subtilis]

3198

YuxO

3199

ComA

3200

ComP

3201
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012

anthracis str. A2012]

3202
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069

3203

ComP

3204

3205
ComQ [Bacillus subtilis]
UniRef100_Q9K5L3

Bacillus subtilis

ComQ

3206

3207
Hypothetical protein yuzC [Bacillus
UniRef100_O32089

Bacillus subtilis

YuzC

subtilis]

3208
Hypothetical protein yuxH [Bacillus
UniRef100_P14203

Bacillus subtilis

YuxH

subtilis]

3209
YueK protein [Bacillus subtilis]
UniRef100_O32090

Bacillus subtilis

YueK

3210
YueJ protein [Bacillus subtilis]
UniRef100_O32091

Bacillus subtilis

YueJ

3211

YueI

3212

3213
Hypothetical protein yueG [Bacillus
UniRef100_O32094

Bacillus subtilis

subtilis]

3214

YueF

3215
RRF2 family protein [Bacillus cereus]
UniRef100_Q81EX1

Bacillus cereus

YwnA

3216
Probable lipase/esterase
UniRef100_Q7UUA2

Rhodopirellula

YuxL

[Rhodopirellula baltica]

baltica

3217
BH1896 protein [Bacillus halodurans]
UniRef100_Q9KBN0

Bacillus halodurans

3218
YueE protein [Bacillus subtilis]
UniRef100_O32098

Bacillus subtilis

YueE

3219
YueD protein [Bacillus subtilis]
UniRef100_O32099

Bacillus subtilis

YueD

3220
Hypothetical protein yueC [Bacillus
UniRef100_O32100

Bacillus subtilis

YueC

subtilis]

3221
YueB protein [Bacillus subtilis]
UniRef100_O32101

Bacillus subtilis

YueB

3222
YukA protein [Bacillus subtilis]
UniRef100_P71068

Bacillus subtilis

YukA

3223
YukC protein [Bacillus subtilis]
UniRef100_P71070

Bacillus subtilis

YukC

3224
YukD protein [Bacillus subtilis]
UniRef100_P71071

Bacillus subtilis

3225
Lin0049 protein [Listeria innocua]
UniRef100_Q92FQ4

Listeria innocua

3226

YflA

3227
YukF protein [Bacillus subtilis]
UniRef100_P71073

Bacillus subtilis

YukF

3228
Alanine dehydrogenase [Bacillus
UniRef100_Q08352

Bacillus subtilis

Ald

subtilis]

3229

3230
YuiH protein [Bacillus subtilis]
UniRef100_O32103

Bacillus subtilis

YuiH

3231
YuiG protein [Bacillus subtilis]
UniRef100_O32104

Bacillus subtilis

YuiG

3232
YuiF protein [Bacillus subtilis]
UniRef100_O32105

Bacillus subtilis

YuiF

3233
Probable cytosol aminopeptidase
UniRef100_O32106

Bacillus subtilis

YuiE

[Bacillus subtilis]

3234
YuiD protein [Bacillus subtilis]
UniRef100_O32107

Bacillus subtilis

YuiD

3235
YuiC protein [Bacillus subtilis]
UniRef100_O32108

Bacillus subtilis

YuiC

3236
YuiB protein [Bacillus subtilis]
UniRef100_O32109

Bacillus subtilis

YuiB

3237

3238

YumB

3239
Thioredoxine reductase [Bacillus
UniRef100_O05268

Bacillus subtilis

YumC

subtilis]

3240

3241
YdjO protein [Bacillus subtilis]
UniRef100_O34759

Bacillus subtilis

3242

YxbD

3243
Hypothetical protein yutM [Bacillus
UniRef100_O32113

Bacillus subtilis

YutM

subtilis]

3244
Diaminopimelate epimerase [Bacillus
UniRef100_O32114

Bacillus subtilis

DapF

subtilis]

3245
YutK protein [Bacillus subtilis]
UniRef100_O32115

Bacillus subtilis

YutK

3246
YuzB protein [Bacillus subtilis]
UniRef100_O32116

Bacillus subtilis

3247
YutJ protein [Bacillus subtilis]
UniRef100_O32117

Bacillus subtilis

YutJ

3248
YdhG protein [Bacillus subtilis]
UniRef100_O05499

Bacillus subtilis

YdhG

3249
Response regulator aspartate
UniRef100_Q9KBE1

Bacillus

RapI

phosphatase [Bacillus halodurans]

halodurans

3250
Phenolic acid decarboxylase [Bacillus
UniRef100_O07006

Bacillus subtilis

PadC

subtilis]

3251
BH2266 protein [Bacillus halodurans]
UniRef100_Q9KAM1

Bacillus halodurans

3252
YuzD protein [Bacillus subtilis]
UniRef100_O32118

Bacillus subtilis

YuzD

3253
YutI protein [Bacillus subtilis]
UniRef100_O32119

Bacillus subtilis

3254
Probable peptidase yuxL [Bacillus
UniRef100_P39839

Bacillus subtilis

YuxL

subtilis]

3255
Homoserine kinase [Bacillus subtilis]
UniRef100_P04948

Bacillus subtilis

ThrB

3256
Threonine synthase [Bacillus subtilis]
UniRef100_P04990

Bacillus subtilis

ThrC

3257
Homoserine dehydrogenase [Bacillus
UniRef100_P19582

Bacillus subtilis

Hom

subtilis]

3258
Glycerate dehydrogenase
UniRef100_Q8ENW9

Oceanobacillus

YvcT

[Oceanobacillus iheyensis]

iheyensis

3259
YutH protein [Bacillus subtilis]
UniRef100_O32123

Bacillus subtilis

YutH

3260
Hypothetical protein yutG [Bacillus
UniRef100_O32124

Bacillus subtilis

YutG

subtilis]

3261
YutF protein [Bacillus subtilis]
UniRef100_O32125

Bacillus subtilis

YutF

3262
YutE protein [Bacillus subtilis]
UniRef100_O32126

Bacillus subtilis

YutE

3263
YutD protein [Bacillus subtilis]
UniRef100_O32127

Bacillus subtilis

3264
YutC protein [Bacillus subtilis]
UniRef100_O32128

Bacillus subtilis

YutC

3265

LipA

3266
YunA protein [Bacillus subtilis]
UniRef100_O32130

Bacillus subtilis

YunA

3267

3268
Sodium-dependent transporter [Bacillus
UniRef100_Q9K7C5

Bacillus

YocR

halodurans]

halodurans

3269
YunB protein [Bacillus subtilis]
UniRef100_O32131

Bacillus subtilis

YunB

3270
YunC protein [Bacillus subtilis]
UniRef100_O32132

Bacillus subtilis

YunC

3271
YunD protein [Bacillus subtilis]
UniRef100_O32133

Bacillus subtilis

YunD

3272
YunE protein [Bacillus subtilis]
UniRef100_O32134

Bacillus subtilis

YunE

3273
YunF protein [Bacillus subtilis]
UniRef100_O32135

Bacillus subtilis

YunF

3274

YmcC

3275
TetR family transcriptional regulator?
UniRef100_Q67KA4

Symbiobacterium

PksA

[Symbiobacterium thermophilum]

thermophilum

3276

3277
Purine catabolism protein pucG
UniRef100_O32148

Bacillus subtilis

YurG

[Bacillus subtilis]

3278
Allantoate amidohydrolase [Bacillus
UniRef100_O32149

Bacillus subtilis

YurH

subtilis]

3279
Purine catabolism regulatory protein
UniRef100_O32138

Bacillus subtilis

PucR

[Bacillus subtilis]

3280
Multidrug resistance protein B [Bacillus
UniRef100_Q63FH7

Bacillus cereus

Blt

cereus ZK]

ZK

3281
BH2308 protein [Bacillus halodurans]
UniRef100_Q9KAH9

Bacillus

YcgA

halodurans

3282

TrpD

3283
Anthranilate phosphoribosyltransferase
UniRef100_Q8U089

Pyrococcus furiosus

[Pyrococcus furiosus]

3284
Extracellular ribonuclease precursor
UniRef100_O32150

Bacillus subtilis

YurI

[Bacillus subtilis]

3285
BH1977 protein [Bacillus halodurans]
UniRef100_Q9KBF1

Bacillus halodurans

3286

YurR

3287
Putative membrane protein [Bordetella
UniRef100_Q7WGW7

Bordetella bronchiseptica

bronchiseptica]

3288
UPI00003CB453 UniRef100 entry
UniRef100_UPI00003CB453

3289
Response regulator aspartate
UniRef100_P96649

Bacillus subtilis

RapI

phosphatase I [Bacillus subtilis]

3290
YurU protein [Bacillus subtilis]
UniRef100_O32162

Bacillus subtilis

YurU

3291
NifU-like protein [Bacillus subtilis]
UniRef100_O32163

Bacillus subtilis

YurV

3292
Probable cysteine desulfurase [Bacillus
UniRef100_O32164

Bacillus subtilis

Csd

subtilis]

3293
YurX protein [Bacillus subtilis]
UniRef100_O32165

Bacillus subtilis

YurX

3294
Vegetative protein 296 [Bacillus subtilis]
UniRef100_P80866

Bacillus subtilis

YurY

3295
Lmo2575 protein [Listeria
UniRef100_Q8Y480

Listeria

CzcD

monocytogenes]

monocytogenes

3296

3297
BH3473 protein [Bacillus halodurans]
UniRef100_Q9K796

Bacillus

YurZ

halodurans

3298
YusA protein [Bacillus subtilis]
UniRef100_O32167

Bacillus subtilis

YusA

3299
YusB protein [Bacillus subtilis]
UniRef100_O32168

Bacillus subtilis

YusB

3300
YusC protein [Bacillus subtilis]
UniRef100_O32169

Bacillus subtilis

YusC

3301
Hypothetical protein yusD [Bacillus
UniRef100_O32170

Bacillus subtilis

YusD

subtilis]

3302
YusE protein [Bacillus subtilis]
UniRef100_O32171

Bacillus subtilis

3303
YusF protein [Bacillus subtilis]
UniRef100_O32172

Bacillus subtilis

YusF

3304

3305
Glycine cleavage system H protein
UniRef100_O32174

Bacillus subtilis

GcvH

[Bacillus subtilis]

3306
Hypothetical protein yusI [Bacillus
UniRef100_O32175

Bacillus subtilis

YusI

subtilis]

3307
YusJ protein [Bacillus subtilis]
UniRef100_O32176

Bacillus subtilis

YusJ

3308
YusK protein [Bacillus subtilis]
UniRef100_O32177

Bacillus subtilis

YusK

3309
YusL protein [Bacillus subtilis]
UniRef100_O32178

Bacillus subtilis

YusL

3310

3311

3312
YusN protein [Bacillus subtilis]
UniRef100_O32180

Bacillus subtilis

YusN

3313
Hypothetical protein yusU [Bacillus
UniRef100_O32187

Bacillus subtilis

subtilis]

3314
BH1040 protein [Bacillus halodurans]
UniRef100_Q9KE18

Bacillus halodurans

3315
YusV protein [Bacillus subtilis]
UniRef100_O32188

Bacillus subtilis

YusV

3316

YfhA

3317
YfiZ protein [Bacillus subtilis]
UniRef100_O31568

Bacillus subtilis

YfiZ

3318
YfiY protein [Bacillus subtilis]
UniRef100_O31567

Bacillus subtilis

YfiY

3319
Hypothetical protein yusW precursor
UniRef100_O32189

Bacillus subtilis

YusW

[Bacillus subtilis]

3320
YusX protein [Bacillus subtilis]
UniRef100_O32190

Bacillus subtilis

YusX

3321
D-alanyl-D-alanine carboxypeptidase
UniRef100_Q8ERG0

Oceanobacillus

DacB

[Oceanobacillus iheyensis]

iheyensis

3322
Hypothetical oxidoreductase yusZ
UniRef100_P37959

Bacillus subtilis

YusZ

[Bacillus subtilis]

3323
Metalloregulation DNA-binding stress
UniRef100_P37960

Bacillus subtilis

MrgA

protein [Bacillus subtilis]

3324
Probable serine protease yvtA [Bacillus
UniRef100_Q9R9I1

Bacillus subtilis

YvtA

subtilis]

3325
Transcriptional regulatory protein cssR
UniRef100_O32192

Bacillus subtilis

CssR

[Bacillus subtilis]

3326
Sensor protein cssS [Bacillus subtilis]
UniRef100_O32193

Bacillus subtilis

CssS

3327
YirB [Bacillus subtilis]
UniRef100_O32302

Bacillus subtilis

3328
Putative HTH-type transcriptional
UniRef100_P40950

Bacillus subtilis

YuxN

regulator yuxN [Bacillus subtilis]

3329
Fumarate hydratase class II [Bacillus
UniRef100_P07343

Bacillus subtilis

CitG

subtilis]

3330

3331
Spore germination protein A1 [Bacillus
UniRef100_P07868

Bacillus subtilis

GerAA

subtilis]

3332
Spore germination protein A2 [Bacillus
UniRef100_P07869

Bacillus subtilis

GerAB

subtilis]

3333
Spore germination protein A3 precursor
UniRef100_P07870

Bacillus subtilis

GerAC

[Bacillus subtilis]

3334

3335

YvqC

3336
YvqE protein [Bacillus subtilis]
UniRef100_O32198

Bacillus subtilis

YvqE

3337
YvqF protein [Bacillus subtilis]
UniRef100_O32199

Bacillus subtilis

YvqF

3338
YvqG protein [Bacillus subtilis]
UniRef100_O32200

Bacillus subtilis

YvqG

3339
Hypothetical protein yvqH [Bacillus
UniRef100_O32201

Bacillus subtilis

YvqH

subtilis]

3340
Hypothetical protein yvqI [Bacillus
UniRef100_O32202

Bacillus subtilis

YvqI

subtilis]

3341
Pectate lyase P358 [Bacillus sp. P-358]
UniRef100_Q8RR73

Bacillus sp. P-358

3342
YvqK protein [Bacillus subtilis]
UniRef100_O34899

Bacillus subtilis

YvqK

3343
UPI00002E3648 UniRef100 entry
UniRef100_UPI00002E3648

FabG

3344

3345

GbsB

3346

DapA

3347
Putative metal binding protein, YvrA
UniRef100_O34631

Bacillus subtilis

YvrA

[Bacillus subtilis]

3348
Putative hemin permease, YvrB
UniRef100_O34451

Bacillus subtilis

YvrB

[Bacillus subtilis]

3349
Putative metal binding protein, YvrC
UniRef100_O34805

Bacillus subtilis

YvrC

[Bacillus subtilis]

3350
Transcriptional regulator, GntR family
UniRef100_Q81SA7

Bacillus

YdhC

[Bacillus anthracis]

anthracis

3351
Putative ketoreductase, YvrD [Bacillus
UniRef100_O34782

Bacillus subtilis

YvrD

subtilis]

3352
UPI00003CC410 UniRef100 entry
UniRef100_UPI00003CC410

YflK

3353
Transcriptional regulators, LysR family
UniRef100_Q81DJ6

Bacillus cereus

AlsR

[Bacillus cereus]

3354
Exo-poly-alpha-D-galacturonosidase,
UniRef100_Q9WYR8

Thermotoga maritima

putative [Thermotoga maritima]

3355
Altronate hydrolase [Bacillus subtilis]
UniRef100_O34673

Bacillus subtilis

UxaA

3356
Altronate oxidoreductase [Bacillus
UniRef100_O34354

Bacillus subtilis

UxaB

subtilis]

3357
LacI repressor-like protein [Bacillus
UniRef100_Q9JMQ1

Bacillus subtilis

ExuR

subtilis]

3358
Hypothetical symporter yjmB [Bacillus
UniRef100_O34961

Bacillus subtilis

YjmB

subtilis]

3359
Uronate isomerase [Bacillus subtilis]
UniRef100_O34808

Bacillus subtilis

UxaC

3360
Putative sensory protein kinase, YvrG
UniRef100_O34989

Bacillus subtilis

YvrG

[Bacillus subtilis]

3361
Putative DNA binding response
UniRef100_P94504

Bacillus subtilis

YvrH

regulator, YvrH [Bacillus subtilis]

3362
Ferrichrome transport ATP-binding
UniRef100_P49938

Bacillus subtilis

FhuC

protein fhuC [Bacillus subtilis]

3363
Ferrichrome transport system
UniRef100_P49937

Bacillus subtilis

FhuG

permease protein fhuG [Bacillus

subtilis]

3364
Ferrichrome transport system
UniRef100_P49936

Bacillus subtilis

FhuB

permease protein fhuB [Bacillus

subtilis]

3365
Putative arginine ornithine antiporter,
UniRef100_O32204

Bacillus subtilis

YvsH

YvsH [Bacillus subtilis]

3366
Hypothetical protein yvsG precursor
UniRef100_O32205

Bacillus subtilis

YvsG

[Bacillus subtilis]

3367
Putative molybdate binding protein,
UniRef100_O32206

Bacillus subtilis

YvgJ

YvgJ [Bacillus subtilis]

3368

YcnB

3369
Putative reductase protein, YvgN
UniRef100_O32210

Bacillus subtilis

YvgN

[Bacillus subtilis]

3370
Fructokinase [Listeria monocytogenes]
UniRef100_Q722A5

Listeria

YdhR

monocytogenes

3371
Hypothetical protein ycbU precursor
UniRef100_P42253

Bacillus subtilis

YcbU

[Bacillus subtilis]

3372
Hypothetical protein CPE0889
UniRef100_Q8XM01

Clostridium perfringens

[Clostridium perfringens]

3373
YvgS protein [Bacillus subtilis]
UniRef100_O32215

Bacillus subtilis

YvgS

3374
Hypothetical UPF0126 protein yvgT
UniRef100_O32216

Bacillus subtilis

YvgT

[Bacillus subtilis]

3375
Glutamate-rich protein grpB [Bacillus
UniRef100_Q81CT5

Bacillus cereus

YqkA

cereus]

3376
Acetyltransferase, GNAT family
UniRef100_Q6HJN8

Bacillus

YuaI

[Bacillus thuringiensis]

thuringiensis

3377
Disulfide bond formation protein C
UniRef100_O32217

Bacillus subtilis

BdbC

[Bacillus subtilis]

3378
Disulfide bond formation protein D
UniRef100_O32218

Bacillus subtilis

BdbD

precursor [Bacillus subtilis]

3379
YvgW protein [Bacillus subtilis]
UniRef100_O32219

Bacillus subtilis

YvgW

3380
ABC transporter, substrate binding
UniRef100_Q8U7T8

Agrobacterium

YvfK

protein [Agrobacterium tumefaciens]

tumefaciens

3381
Putative sugar transport integral
UniRef100_Q9K442

Streptomyces

YurN

membrane protein [Streptomyces

coelicolor

coelicolor]

3382
Putative sugar transport integral
UniRef100_Q9K441

Streptomyces

YurM

membrane protein [Streptomyces

coelicolor

coelicolor]

3383
Alpha-glucosidase [Clostridium
UniRef100_Q97K36

Clostridium acetobutylicum

acetobutylicum]

3384
Glucan-glucohydrolase [Microbispora
UniRef100_Q59506

Microbispora

YbbD

bispora]

bispora

3385
ABC transporter, ATP-binding protein
UniRef100_Q81NK1

Bacillus

MntB

[Bacillus anthracis]

anthracis

3386
ABC transporter, permease protein
UniRef100_Q81NK2

Bacillus

MntD

[Bacillus anthracis]

anthracis

3387
ABC transporter, manganese-binding
UniRef100_Q71YG9

Listeria

YcdH

protein [Listeria monocytogenes]

monocytogenes

3388
Hypothetical protein [Bacillus cereus]
UniRef100_Q74NQ1

Bacillus cereus

YhjQ

3389
Phosphosugar-binding transcriptional
UniRef100_Q837Y3

Enterococcus

YfiA

regulator, RpiR family, putative

faecalis

[Enterococcus faecalis]

3390
1-phosphofructokinase [Enterococcus
UniRef100_Q837Y2

Enterococcus

FruK

faecalis]

faecalis

3391
PTS system, fructose-specific family,
UniRef100_Q837Y1

Enterococcus

FruA

IIBC components [Enterococcus

faecalis

faecalis]

3392
PTS system, IIA component
UniRef100_Q837Y0

Enterococcus

FruA

[Enterococcus faecalis]

faecalis

3393
Fructose/tagatose bisphosphate
UniRef100_Q7MC78

Vibrio vulnificus

FbaA

aldolase [Vibrio vulnificus]

3394

3395
Heavy metal-transporting ATPase
UniRef100_Q6HF81

Bacillus

YvgX

[Bacillus thuringiensis]

thuringiensis

3396
Copper chaperone copZ [Bacillus
UniRef100_O32221

Bacillus subtilis

subtilis]

3397
YvgZ protein [Bacillus subtilis]
UniRef100_O32222

Bacillus subtilis

YvgZ

3398
Intracellular proteinase [Thermoplasma
UniRef100_Q97BN0

Thermoplasma

YraA

volcanium]

volcanium

3399
Putative HTH-type transcriptional
UniRef100_O05261

Bacillus subtilis

YulB

regulator yulB [Bacillus subtilis]

3400
Rhamnulose kinase/L-fuculose kinase
UniRef100_Q8A1A3

Bacteroides

YulC

[Bacteroides thetaiotaomicron]

thetaiotaomicron

3401
Putative sugar isomerase
UniRef100_Q9XAB3

Streptomyces coelicolor

[Streptomyces coelicolor]

3402
Hypothetical protein [Polyangium
UniRef100_Q9L8B7

Polyangium cellulosum

cellulosum]

3403
Hypothetical oxidoreductase yuxG
UniRef100_P40747

Bacillus subtilis

YuxG

[Bacillus subtilis]

3404
YfhI protein [Bacillus subtilis]
UniRef100_O31577

Bacillus subtilis

YfhI

3405
YvmA [Bacillus subtilis]
UniRef100_O34307

Bacillus subtilis

YvmA

3406
YvmC [Bacillus subtilis]
UniRef100_O34351

Bacillus subtilis

YvmC

3407
Putative cytochrome P450 CYPX
UniRef100_O34926

Bacillus subtilis

CypX

[Bacillus subtilis]

3408
Hypothetical protein [Chromobacterium
UniRef100_Q7NRR6

Chromobacterium violaceum

violaceum]

3409

YvnA

3410
Hypothetical protein BRA0099 [Brucella
UniRef100_Q8FXH8

Brucella suis

suis]

3411
Chloroplast Toc64-2 [Physcomitrella
UniRef100_Q6RJN6

Physcomitrella

GatA

patens]

patens

3412
Hypothetical oxidoreductase yvaA
UniRef100_O32223

Bacillus subtilis

YvaA

[Bacillus subtilis]

3413
Putative acyl carrier protein
UniRef100_O32224

Bacillus subtilis

YvaB

phosphodiesterase 2 [Bacillus subtilis]

3414
Hypothetical UPF0176 protein ybfQ
UniRef100_O31457

Bacillus subtilis

YbfQ

[Bacillus subtilis]

3415
Putative DNA binding response
UniRef100_P94504

Bacillus subtilis

regulator, YvrH [Bacillus subtilis]

3416
YvrI protein [Bacillus subtilis]
UniRef100_O34843

Bacillus subtilis

YvrI

3417

3418
Oxalate decarboxylase oxdC [Bacillus
UniRef100_O34714

Bacillus subtilis

OxdC

subtilis]

3419
Hypothetical protein yvrL [Bacillus
UniRef100_O34686

Bacillus subtilis

YvrL

subtilis]

3420
BH3120 protein [Bacillus halodurans]
UniRef100_Q9K886

Bacillus halodurans

3421

3422
Group-specific protein [Bacillus cereus
UniRef100_Q633V1

Bacillus cereus ZK

ZK]

3423

3424

3425

3426
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069

3427
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012

anthracis str. A2012]

3428
LexA repressor [Listeria
UniRef100_Q720B9

Listeria monocytogenes

monocytogenes]

3429
Hypothetical protein [Bacillus cereus
UniRef100_Q630E5

Bacillus cereus ZK

ZK]

3430
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81XX1

Bacillus anthracis

3431

3432
Hypothetical protein RSc1705
UniRef100_Q8XYQ3

Ralstonia solanacearum

[Ralstonia solanacearum]

3433

YxiD

3434
Hypothetical protein [Bacillus
UniRef100_Q6HH72

Bacillus thuringiensis

thuringiensis]

3435

BlyA

3436

3437

3438

3439
YomQ protein [Bacteriophage SPBc2]
UniRef100_O64053
Bacteriophage
YomQ

SPBc2

3440

XkdV

3441

3442

3443

YubE

3444
ORF38 [Bacteriophage phi-105]
UniRef100_Q9ZXE5
Bacteriophage phi-105

3445

YqbO

3446
Hypothetical protein MW1895
UniRef100_Q8NVQ7

Staphylococcus aureus

[Staphylococcus aureus]

3447

3448

3449
Prophage LambdaBa02, major tail
UniRef100_Q81W97

Bacillus anthracis

protein, putative [Bacillus anthracis]

3450

3451
ORF32 [Bacteriophage phi-105]
UniRef100_Q9ZXF1
Bacteriophage phi-105

3452

3453
Gp7 protein [Bacteriophage phi3626]
UniRef100_Q8SBP7
Bacteriophage phi3626

3454
ORF29 [Bacteriophage phi-105]
UniRef100_Q9ZXF4
Bacteriophage phi-105

3455
ORF27 [Bacteriophage phi-105]
UniRef100_Q9ZXF6
Bacteriophage phi-105

3456
ClpP family serine protease, possible
UniRef100_Q97HW4

Clostridium

ClpP

phage related [Clostridium

acetobutylicum

acetobutylicum]

3457
ORF25 [Bacteriophage phi-105]
UniRef100_Q9ZXF8
Bacteriophage phi-105

3458

3459
Putative terminase large subunit
UniRef100_Q8SBQ2
Bacteriophage phi3626

[Bacteriophage phi3626]

3460
Phage terminase-like protein, small
UniRef100_Q97HW1

Clostridium acetobutylicum

subunit [Clostridium acetobutylicum]

3461
Prophage LambdaBa02, HNH
UniRef100_Q81W86

Bacillus anthracis

endonuclease family protein [Bacillus

anthracis]

3462
Spore coat protein D [Bacillus subtilis]
UniRef100_P07791

Bacillus subtilis

3463
ORF16 [Bacteriophage phi-105]
UniRef100_Q9ZXB9
Bacteriophage phi-105

3464

3465

3466
ORF14 [Bacteriophage phi-105]
UniRef100_Q9ZXC1
Bacteriophage phi-105

3467
ORF13 [Bacteriophage phi-105]
UniRef100_Q9ZXC2
Bacteriophage phi-105

3468
ORF12 [Bacteriophage phi-105]
UniRef100_Q9ZXC3
Bacteriophage phi-105

3469

3470
ORF11 [Bacteriophage phi-105]
UniRef100_Q9ZXC4
Bacteriophage phi-105

3471
ORF10 [Bacteriophage phi-105]
UniRef100_Q9ZXC5
Bacteriophage phi-105

3472
DNA, complete sequence
UniRef100_Q9ZXC7
Bacteriophage phi-105

[Bacteriophage phi-105]

3473
DNA, complete sequence
UniRef100_Q9ZXC8
Bacteriophage phi-105

[Bacteriophage phi-105]

3474

3475

3476

3477
Orf 36 [Staphylococcus aureus
UniRef100_Q9T1Z5

Staphylococcus aureus

bacteriophage PVL]

bacteriophage PVL

3478

3479
Orf16 [Bacteriophage bIL312]
UniRef100_Q9AZE4
Bacteriophage bIL312

3480
Gp26 protein [Bacteriophage phi3626]
UniRef100_Q8SBM8
Bacteriophage phi3626

3481
Cl like protein [Lactobacillus casei
UniRef100_O64370

Lactobacillus casei

bacteriophage A2]

bacteriophage A2

3482
Int gene product [Staphylococcus
UniRef100_Q932E5

Staphylococcus

YdcL

aureus]

aureus

3483
SsrA-binding protein [Bacillus subtilis]
UniRef100_O32230

Bacillus subtilis

SmpB

3484
Ribonuclease R [Bacillus subtilis]
UniRef100_O32231

Bacillus subtilis

Rnr

3485
YvaK protein [Bacillus subtilis]
UniRef100_O32232

Bacillus subtilis

YvaK

3486

3487

3488

3489

YvaO

3490

YvaO

3491
Glycine betaine/carnitine/choline
UniRef100_O34742

Bacillus subtilis

OpuCD

transport system permease protein

opuCD [Bacillus subtilis]

3492
Glycine betaine/carnitine/choline-
UniRef100_O32243

Bacillus subtilis

OpuCC

binding protein precursor [Bacillus

subtilis]

3493
Glycine betaine/carnitine/choline
UniRef100_O34878

Bacillus subtilis

OpuCB

transport system permease protein

opuCB [Bacillus subtilis]

3494
Glycine betaine/carnitine/choline
UniRef100_O34992

Bacillus subtilis

OpuCA

transport ATP-binding protein opuCA

[Bacillus subtilis]

3495
ORF-1 [Bacillus subtilis]
UniRef100_O34709

Bacillus subtilis

YvaV

3496

YvbG

3497

YvbI

3498
YvbJ protein [Bacillus subtilis]
UniRef100_O32247

Bacillus subtilis

YvbJ

3499
UPI00003CBDCC UniRef100 entry
UniRef100_UPI00003CBDCC

YfiY

3500

3501
YoaZ [Bacillus subtilis]
UniRef100_O34947

Bacillus subtilis

YoaZ

3502
Enolase [Bacillus subtilis]
UniRef100_P37869

Bacillus subtilis

Eno

3503
2,3-bisphosphoglycerate-independent
UniRef100_P39773

Bacillus subtilis

Pgm

phosphoglycerate mutase [Bacillus

subtilis]

3504
Triosephosphate isomerase [Bacillus
UniRef100_P27876

Bacillus subtilis

TpiA

subtilis]

3505
Phosphoglycerate kinase [Bacillus
UniRef100_P40924

Bacillus subtilis

Pgk

subtilis]

3506
Glyceraldehyde-3-phosphate
UniRef100_P09124

Bacillus subtilis

GapA

dehydrogenase 1 [Bacillus subtilis]

3507
Central glycolytic genes regulator
UniRef100_O32253

Bacillus subtilis

CggR

[Bacillus subtilis]

3508
Hypothetical protein yvbT [Bacillus
UniRef100_O32254

Bacillus subtilis

YvbT

subtilis]

3509
YvbW protein [Bacillus subtilis]
UniRef100_O32257

Bacillus subtilis

YvbW

3510

3511
ABC-type multidrug transport system,
UniRef100_Q8R7K3

Thermoanaerobacter

YfiL

ATPase component

tengcongensis

[Thermoanaerobacter tengcongensis]

3512

3513
YvbY protein [Bacillus subtilis]
UniRef100_O32259

Bacillus subtilis

YvbY

3514
YvfW protein [Bacillus subtilis]
UniRef100_O07021

Bacillus subtilis

YvfW

3515

YvfV

3516
Hypothetical protein yvfI [Bacillus
UniRef100_O07007

Bacillus subtilis

YvfI

subtilis]

3517
Putative L-lactate permease yvfH
UniRef100_P71067

Bacillus subtilis

YvfH

[Bacillus subtilis]

3518
RNA polymerase sigma-54 factor
UniRef100_P24219

Bacillus subtilis

SigL

[Bacillus subtilis]

3519
Hypothetical protein yvfG [Bacillus
UniRef100_P71066

Bacillus subtilis

subtilis]

3520
Hypothetical protein yvfF [Bacillus
UniRef100_P71065

Bacillus subtilis

YvfF

subtilis]

3521
YvfE protein [Bacillus subtilis]
UniRef100_Q795J3

Bacillus subtilis

YvfE

3522
Hypothetical protein yvfD [Bacillus
UniRef100_P71063

Bacillus subtilis

YvfD

subtilis]

3523
Hypothetical protein yvfC [Bacillus
UniRef100_P71062

Bacillus subtilis

YvfC

subtilis]

3524

YvfB

3525

YvfA

3526
Hypothetical protein yveT [Bacillus
UniRef100_P71059

Bacillus subtilis

YveT

subtilis]

3527
Hypothetical protein yveS [Bacillus
UniRef100_P71058

Bacillus subtilis

YveS

subtilis]

3528
Hypothetical protein yveR [Bacillus
UniRef100_P71057

Bacillus subtilis

YveR

subtilis]

3529
Hypothetical protein yveQ [Bacillus
UniRef100_P71056

Bacillus subtilis

YveQ

subtilis]

3530
Hypothetical protein yveP [Bacillus
UniRef100_P71055

Bacillus subtilis

YveP

subtilis]

3531
Hypothetical protein yveO [Bacillus
UniRef100_P71054

Bacillus subtilis

YveO

subtilis]

3532
Hypothetical protein yveN [Bacillus
UniRef100_P71053

Bacillus subtilis

YveN

subtilis]

3533
Hypothetical protein yveM [Bacillus
UniRef100_P71052

Bacillus subtilis

YveM

subtilis]

3534

YveL

3535
Chain length determinant protein
UniRef100_Q72XH7

Bacillus cereus

YveK

[Bacillus cereus]

3536
Hypothetical protein yveJ [Bacillus
UniRef100_P71049

Bacillus subtilis

Slr

subtilis]

3537
General stress protein 14 [Bacillus
UniRef100_P80871

Bacillus subtilis

YwrO

subtilis]

3538

YcbE

3539
UPI000027D43B UniRef100 entry
UniRef100_UPI000027D43B

YvcT

3540
4-hydroxythreonine-4-phosphate
UniRef100_Q8CUU4

Oceanobacillus iheyensis

dehydrogenase (EC 1.1.1.262) (4-

(phosphohydroxy)-L-threonine

dehydrogenase) [Oceanobacillus

iheyensis]

3541
Hypothetical conserved protein
UniRef100_Q8CUU5

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

3542

GbsB

3543

DapA

3544
Transcriptional regulator
UniRef100_Q8CUU7

Oceanobacillus

RocR

[Oceanobacillus iheyensis]

iheyensis

3545
Hypothetical protein [Bdellovibrio
UniRef100_Q6MH73

Bdellovibrio bacteriovorus

bacteriovorus]

3546
NADH-dependent flavin oxidoreductase
UniRef100_Q8ELB6

Oceanobacillus

YqiG

[Oceanobacillus iheyensis]

iheyensis

3547
Levansucrase precursor [Bacillus
UniRef100_P05655

Bacillus subtilis

SacB

subtilis]

3548
Hypothetical protein yveB [Bacillus
UniRef100_O07003

Bacillus subtilis

YveB

subtilis]

3549
Hypothetical protein [Pseudomonas
UniRef100_Q9I5B4

Pseudomonas aeruginosa

aeruginosa]

3550
ATP-dependent Clp protease
UniRef100_P80244

Bacillus subtilis

ClpP

proteolytic subunit [Bacillus subtilis]

3551
Hypothetical protein yvdD [Bacillus
UniRef100_O06986

Bacillus subtilis

YvdD

subtilis]

3552

3553
Possible metal-dependent
UniRef100_Q6HM73

Bacillus

YdhJ

phosphohydrolase; possible oxetanocin

thuringiensis

A resistance protein [Bacillus

thuringiensis]

3554
Hypothetical protein yvdB [Bacillus
UniRef100_O06984

Bacillus subtilis

YvdB

subtilis]

3555
Hypothetical protein yvdA [Bacillus
UniRef100_O06983

Bacillus subtilis

YvdA

subtilis]

3556
Probable 2-ketogluconate reductase
UniRef100_O32264

Bacillus subtilis

YvcT

[Bacillus subtilis]

3557
BH0392 protein [Bacillus halodurans]
UniRef100_Q9KFT5

Bacillus

YdfE

halodurans

3558
BH0391 protein [Bacillus halodurans]
UniRef100_Q9KFT6

Bacillus

YdfF

halodurans

3559
Ribonuclease [Paenibacillus polymyxa]
UniRef100_Q7MOX3

Paenibacillus polymyxa

3560
Barstar [Bacillus amyloliquefaciens]
UniRef100_P11540

Bacillus amyloliquefaciens

3561
Hypothetical protein yvcN [Bacillus
UniRef100_O06977

Bacillus subtilis

YvcN

subtilis]

3562
HPr-like protein crh [Bacillus subtilis]
UniRef100_O06976

Bacillus subtilis

3563
Hypothetical protein yvcL [Bacillus
UniRef100_O06975

Bacillus subtilis

YvcL

subtilis]

3564
Hypothetical UPF0052 protein yvcK
UniRef100_O06974

Bacillus subtilis

YvcK

[Bacillus subtilis]

3565
Hypothetical UPF0042 protein yvcJ
UniRef100_O06973

Bacillus subtilis

YvcJ

[Bacillus subtilis]

3566
Hypothetical protein yvcI [Bacillus
UniRef100_O06972

Bacillus subtilis

YvcI

subtilis]

3567
Thioredoxin reductase [Bacillus subtilis]
UniRef100_P80880

Bacillus subtilis

TrxB

3568
Hypothetical protein yvcD [Bacillus
UniRef100_O06968

Bacillus subtilis

YvcD

subtilis]

3569
Hypothetical conserved protein
UniRef100_Q8ETL1

Oceanobacillus

YbbJ

[Oceanobacillus iheyensis]

iheyensis

3570
Histidine biosynthesis bifunctional
UniRef100_O34912
Includes;
HisI

protein hisIE [Includes; Phosphoribosyl-

Phosphoribosyl-

AMP cyclohydrolase (EC 3.5.4.19)

AMP

(PRA-CH); Phosphoribosyl-ATP

cyclohydrolase

pyrophosphatase (EC 3.6.1.31) (PRA-

(EC 3.5.4.19)

PH)] [Bacillus subtilis]

(PRA-CH);

Phosphoribosyl-

ATP

pyrophosphatase

(EC 3.6.1.31)

(PRA-PH)

3571
Imidazole glycerol phosphate synthase
UniRef100_O34727

Bacillus subtilis

HisF

subunit hisF [Bacillus subtilis]

3572
1-(5-phosphoribosyl)-5-[(5-
UniRef100_O35006
(5-
HisA

phosphoribosylamino)methylideneamino]

phosphoribosylamino)

imidazole-4-carboxamide isomerase

methylideneamino

[Bacillus subtilis]

3573
Imidazole glycerol phosphate synthase
UniRef100_O34565

Bacillus subtilis

HisH

subunit hisH [Bacillus subtilis]

3574
Imidazoleglycerol-phosphate
UniRef100_O34683

Bacillus subtilis

HisB

dehydratase [Bacillus subtilis]

3575
Histidinol dehydrogenase [Bacillus
UniRef100_O34651

Bacillus subtilis

HisD

subtilis]

3576
ATP phosphoribosyltransferase
UniRef100_O34520

Bacillus subtilis

HisG

[Bacillus subtilis]

3577

HisZ

3578
YvpB [Bacillus subtilis]
UniRef100_O34735

Bacillus subtilis

YvpB

3579
Pectate lyase Pel-28K [Bacillus sp. P-
UniRef100_Q8L0R5

Bacillus sp. P-
YvpA

2850]

2850

3580
Putative acetyltransferase [Bacillus
UniRef100_O34993

Bacillus subtilis

YvoF

subtilis]

3581
Pyrophosphatase ppaX [Bacillus
UniRef100_Q9JMQ2

Bacillus subtilis

HprP

subtilis]

3582
YvoD [Bacillus subtilis]
UniRef100_O34382

Bacillus subtilis

YvoD

3583
Prolipoprotein diacylglyceryl transferase
UniRef100_O34752

Bacillus subtilis

Lgt

[Bacillus subtilis]

3584
HPr kinase/phosphorylase (EC 2.7.1.—)
UniRef100_O34483

Bacillus subtilis

HprK

(EC 2.7.4.—) (HPrK/P) (HPr(Ser)

kinase/phosphorylase) [Bacillus subtilis]

3585
YfiV protein [Bacillus subtilis]
UniRef100_O31564

Bacillus subtilis

YfiV

3586
YfiU protein [Bacillus subtilis]
UniRef100_O31563

Bacillus subtilis

YfiU

3587
YvnB [Bacillus subtilis]
UniRef100_O34986

Bacillus subtilis

YvnB

3588
YvlD [Bacillus subtilis]
UniRef100_O34648

Bacillus subtilis

YvlD

3589
YvlC [Bacillus subtilis]
UniRef100_O34719

Bacillus subtilis

3590
YvlB [Bacillus subtilis]
UniRef100_O34628

Bacillus subtilis

YvlB

3591
Hypothetical protein yvlA [Bacillus
UniRef100_O34322

Bacillus subtilis

YvlA

subtilis]

3592
Hypothetical protein yvkN [Bacillus
UniRef100_O34604

Bacillus subtilis

subtilis]

3593

3594
UvrABC system protein A [Bacillus
UniRef100_O34863

Bacillus subtilis

UvrA

subtilis]

3595
UvrABC system protein B [Bacillus
UniRef100_Q659H3

Bacillus

UvrB

amyloliquefaciens]

amyloliquefaciens

3596
CsbA protein [Bacillus subtilis]
UniRef100_P37953

Bacillus subtilis

3597

3598
YvjD [Bacillus subtilis]
UniRef100_O34375

Bacillus subtilis

YvjD

3599

YvzD

3600
Putative protease [Bacillus subtilis]
UniRef100_O35002

Bacillus subtilis

YvjB

3601
Tartrate dehydrogenase
UniRef100_Q8ESH5

Oceanobacillus

YcsA

[Oceanobacillus iheyensis]

iheyensis

3602
Cell wall-binding protein [Bacillus
UniRef100_Q9K6X4

Bacillus

YvcE

halodurans]

halodurans

3603
Cell division protein ftsX homolog
UniRef100_O34876

Bacillus subtilis

FtsX

[Bacillus subtilis]

3604
Cell division ATP-binding protein
UniRef100_O34814

Bacillus subtilis

FtsE

[Bacillus subtilis]

3605
Cytochrome c-551 [Bacillus subtilis]
UniRef100_O34594

Bacillus subtilis

CccB

3606
YvjA [Bacillus subtilis]
UniRef100_O34792

Bacillus subtilis

YvjA

3607
Peptide chain release factor 2 [Bacillus
UniRef100_P28367

Bacillus subtilis

PrfB

subtilis]

3608
Preprotein translocase secA subunit
UniRef100_P28366

Bacillus subtilis

SecA

[Bacillus subtilis]

3609
Hypothetical protein yvyD [Bacillus
UniRef100_P28368

Bacillus subtilis

YvyD

subtilis]

3610

3611
Flagellar protein fliT [Bacillus subtilis]
UniRef100_P39740

Bacillus subtilis

FliT

3612
Flagellar protein fliS [Bacillus subtilis]
UniRef100_P39739

Bacillus subtilis

FliS

3613
Flagellar hook-associated protein 2
UniRef100_P39738

Bacillus subtilis

FliD

[Bacillus subtilis]

3614
Hypothetical protein yvyC [Bacillus
UniRef100_P39737

Bacillus subtilis

YvyC

subtilis]

3615
Flagellin [Bacillus subtilis]
UniRef100_P02968

Bacillus subtilis

Hag

3616
Carbon storage regulator homolog
UniRef100_P33911

Bacillus subtilis

[Bacillus subtilis]

3617
Transmembrane protein [Bacillus
UniRef100_P96503

Bacillus subtilis

YviF

subtilis]

3618
YviE protein [Bacillus subtilis]
UniRef100_P96502

Bacillus subtilis

YviE

3619
Flagellar hook-associated protein 3
UniRef100_P96501

Bacillus subtilis

FlgL

[Bacillus subtilis]

3620
Flagellar hook-associated protein 1
UniRef100_P39810

Bacillus subtilis

FlgK

[Bacillus subtilis]

3621
Hypothetical protein yvyG [Bacillus
UniRef100_P39808

Bacillus subtilis

YvyG

subtilis]

3622
Negative regulator of flagellin synthesis
UniRef100_P39809

Bacillus subtilis

[Bacillus subtilis]

3623
Hypothetical protein yvyF [Bacillus
UniRef100_P39807

Bacillus subtilis

YvyF

subtilis]

3624

ComFC

3625

3626

ComFA

3627
DegV protein [Bacillus subtilis]
UniRef100_P32436

Bacillus subtilis

YviA

3628
Transcriptional regulatory protein degU
UniRef100_P13800

Bacillus subtilis

DegU

[Bacillus subtilis]

3629

DegS

3630
Hypothetical UPF0029 protein yvyE
UniRef100_P32437

Bacillus subtilis

YvyE

[Bacillus subtilis]

3631
Putative transcriptional regulator
UniRef100_P96499

Bacillus subtilis

YvhJ

[Bacillus subtilis]

3632
Hypothetical protein yunG [Bacillus
UniRef100_Q63CU2

Bacillus cereus

YunG

cereus ZK]

ZK

3633
Probable undecaprenyl-phosphate N-
UniRef100_O34753
Bacillus subtilis
TagO

acetylglucosaminyl 1-phosphate

transferase [Bacillus subtilis]

3634
Putative teichuronic acid biosynthesis
UniRef100_O32267

Bacillus subtilis

TuaH

glycosyl transferase tuaH [Bacillus

subtilis]

3635
Putative teichuronic acid biosynthesis
UniRef100_O32268

Bacillus subtilis

TuaG

glycosyl transferase tuaG [Bacillus

subtilis]

3636
Teichuronic acid biosynthesis protein
UniRef100_O32269

Bacillus subtilis

TuaF

tuaF [Bacillus subtilis]

3637
Teichuronic acid biosynthesis protein
UniRef100_O32270

Bacillus subtilis

TuaE

tuaE [Bacillus subtilis]

3638
Putative UDP-glucose 4-epimerase
UniRef100_Q57664

Methanococcus

GalE

[Methanococcus jannaschii]

jannaschii

3639
UDP-glucose 6-dehydrogenase
UniRef100_O32271

Bacillus subtilis

TuaD

[Bacillus subtilis]

3640
Putative teichuronic acid biosynthesis
UniRef100_O32272

Bacillus subtilis

TuaC

glycosyl transferase tuaC [Bacillus

subtilis]

3641
Teichuronic acid biosynthesis protein
UniRef100_O32273

Bacillus subtilis

TuaB

tuaB [Bacillus subtilis]

3642
UPI00003CB85E UniRef100 entry
UniRef100_UPI00003CB85E

TuaA

3643

LytC

3644
Amidase enhancer precursor [Bacillus
UniRef100_Q02113

Bacillus subtilis

LytB

subtilis]

3645

LytA

3646
Membrane-bound protein lytR [Bacillus
UniRef100_Q02115

Bacillus subtilis

LytR

subtilis]

3647
UDP-N-acetylglucosamine 2-epimerase
UniRef100_P39131

Bacillus subtilis

YvyH

[Bacillus subtilis]

3648
UTP--glucose-1-phosphate
UniRef100_Q05852

Bacillus subtilis

GtaB

uridylyltransferase [Bacillus subtilis]

3649

TagH

3650
Teichoic acid translocation permease
UniRef100_P42953

Bacillus subtilis

TagG

protein tagG [Bacillus subtilis]

3651
Teichoic acid biosynthesis protein
UniRef100_Q9CH20

Lactococcus

TagF

[Lactococcus lactis]

lactis

3652
CDP-glycerol: poly(glycerophosphate)
UniRef100_P13485

Bacillus subtilis

TagF

glycerophosphotransferase [Bacillus

subtilis]

3653

TagD

3654

TagA

3655
Putative CDP-
UniRef100_P27621

Bacillus subtilis

TagB

glycerol:glycerophosphate

glycerophosphotransferase [Bacillus

subtilis]

3656
Beta-N-acetylglucosaminidase
UniRef100_P39848

Bacillus subtilis

LytD

precursor [Bacillus subtilis]

3657
Mannose-6-phosphate isomerase
UniRef100_P39841

Bacillus subtilis

Pmi

[Bacillus subtilis]

3658

3659
Anaerobic ribonucleoside-triphosphate
UniRef100_Q733N6

Bacillus cereus

reductase, putative [Bacillus cereus]

3660
2-keto-3-deoxygluconate permease
UniRef100_P50847

Bacillus subtilis

KdgT

[Bacillus subtilis]

3661
KHG/KDPG aldolase [Includes: 4-
UniRef100_P50846
Includes: 4-
KdgA

hydroxy-2-oxoglutarate aldolase (EC

hydroxy-2-

4.1.3.16) (2-keto-4-hydroxyglutarate

oxoglutarate

aldolase) (KHG-aldolase); 2-dehydro-

aldolase (EC

3-deoxy-phosphogluconate aldolase

4.1.3.16) (2-keto-

(EC 4.1.2.14) (Phospho-2-dehydro-3-

4-

deoxygluconate aldolase) (Phospho-2-

hydroxyglutarate

keto-3-d

aldolase) (KHG-

aldolase); 2-

dehydro-3-

deoxy-

phosphogluconate

aldolase (EC

4.1.2.14)

(Phospho-2-

dehydro-3-

deoxygluconate

aldolase)

(Phospho-2-keto-

3-

deoxygluconate

aldol

3662
2-dehydro-3-deoxygluconokinase
UniRef100_P50845

Bacillus subtilis

KdgK

[Bacillus subtilis]

3663
HTH-type transcriptional regulator kdgR
UniRef100_P50844

Bacillus subtilis

KdgR

[Bacillus subtilis]

3664

KduI

3665
2-deoxy-D-gluconate 3-dehydrogenase
UniRef100_P50842

Bacillus subtilis

KduD

[Bacillus subtilis]

3666
Alcohol dehydrogenase, glutathione-
UniRef100_Q6HGX1

Bacillus

AdhB

dependent formaldehyde

thuringiensis

dehydrogenase [Bacillus thuringiensis]

3667
YwtG protein [Bacillus subtilis]
UniRef100_P96742

Bacillus subtilis

YwtG

3668
Hypothetical transcriptional regulator
UniRef100_Q7WY78

Bacillus subtilis

YwtF

ywtF [Bacillus subtilis]

3669
YwtE protein [Bacillus subtilis]
UniRef100_P96741

Bacillus subtilis

YwtE

3670
Gamma-DL-glutamyl hydrolase
UniRef100_P96740

Bacillus subtilis

YwtD

precursor [Bacillus subtilis]

3671

3672
YwtB protein [Bacillus subtilis]
UniRef100_P96738

Bacillus subtilis

YwtB

3673
YwtA protein [Bacillus subtilis]
UniRef100_P96737

Bacillus subtilis

YwtA

3674
YwsC protein [Bacillus subtilis]
UniRef100_P96736

Bacillus subtilis

YwsC

3675

RbsR

3676
Ribokinase [Bacillus subtilis]
UniRef100_P36945

Bacillus subtilis

RbsK

3677
High affinity ribose transport protein
UniRef100_P36946

Bacillus subtilis

RbsD

rbsD [Bacillus subtilis]

3678
Ribose transport ATP-binding protein
UniRef100_P36947

Bacillus subtilis

RbsA

rbsA [Bacillus subtilis]

3679
Ribose transport system permease
UniRef100_P36948

Bacillus subtilis

RbsC

protein rbsC [Bacillus subtilis]

3680
D-ribose-binding protein precursor
UniRef100_P36949

Bacillus subtilis

RbsB

[Bacillus subtilis]

3681
Alpha-acetolactate decarboxylase
UniRef100_Q04777

Bacillus subtilis

AlsD

[Bacillus subtilis]

3682
Acetolactate synthase [Bacillus subtilis]
UniRef100_Q04789

Bacillus subtilis

AlsS

3683
HTH-type transcriptional regulator alsR
UniRef100_Q04778

Bacillus subtilis

AlsR

[Bacillus subtilis]

3684
YwrD protein [Bacillus subtilis]
UniRef100_O05218

Bacillus subtilis

YwrD

3685
YwrC protein [Bacillus subtilis]
UniRef100_O05217

Bacillus subtilis

YwrC

3686
YwrB protein [Bacillus subtilis]
UniRef100_O05216

Bacillus subtilis

YwrB

3687
YwrA protein [Bacillus subtilis]
UniRef100_O05215

Bacillus subtilis

YwrA

3688
Putative protein-tyrosine phosphatase
UniRef100_P96717

Bacillus subtilis

YwqE

ywqE [Bacillus subtilis]

3689
Hypothetical protein ywqC [Bacillus
UniRef100_P96715

Bacillus subtilis

YwqC

subtilis]

3690
Hypothetical protein ywqB [Bacillus
UniRef100_P96714

Bacillus subtilis

YwqB

subtilis]

3691
YwqA protein [Bacillus subtilis]
UniRef100_P94593

Bacillus subtilis

YwqA

3692
YwpJ protein [Bacillus subtilis]
UniRef100_P94592

Bacillus subtilis

YwpJ

3693
YwpI protein [Bacillus subtilis]
UniRef100_P94591

Bacillus subtilis

GlcR

3694
Fructose-6-phosphate aldolase 2
UniRef100_P58424

Escherichia coli

YwjH

[Escherichia coli O157:H7]

O157:H7

3695
Hypothetical protein PM1968
UniRef100_Q9CJM9

Pasteurella

FabG

[Pasteurella multocida]

multocida

3696
PTS system, sorbitol-specific IIA
UniRef100_Q82YX6

Enterococcus faecalis

component [Enterococcus faecalis]

3697
PTS system, sorbitol-specific IIBC
UniRef100_Q82YX5

Enterococcus faecalis

components [Enterococcus faecalis]

3698
UPI00002F4A3A UniRef100 entry
UniRef100_UPI00002F4A3A

3699
Sorbitol operon activator
UniRef100_Q9X673

Streptococcus mutans

[Streptococcus mutans]

3700
Putative transcriptional regulator of
UniRef100_Q83PA8

Shigella flexneri

DeoR

sorbose uptake and utilization genes

[Shigella flexneri]

3701
YwpH protein [Bacillus subtilis]
UniRef100_P94590

Bacillus subtilis

YwpH

3702
Hypothetical protein ywpG [Bacillus
UniRef100_P94589

Bacillus subtilis

YwpG

subtilis]

3703
YwpF protein [Bacillus subtilis]
UniRef100_P94588

Bacillus subtilis

YwpF

3704
Large-conductance mechanosensitive
UniRef100_P94585

Bacillus subtilis

MscL

channel [Bacillus subtilis]

3705
(3R)-hydroxymyristoyl-[acyl carrier
UniRef100_Q81JE0
acyl carrier
YwpB

protein] dehydratase (EC 4.2.1.—) ((3R)-

protein

hydroxymyristoyl ACP dehydrase)

[Bacillus anthracis]

3706
Flagellar hook-basal body complex
UniRef100_P39753

Bacillus subtilis

FlhP

protein flhP [Bacillus subtilis]

3707
Flagellar hook-basal body complex
UniRef100_P39752

Bacillus subtilis

FlhO

protein flhO [Bacillus subtilis]

3708
MreB-like protein [Bacillus subtilis]
UniRef100_P39751

Bacillus subtilis

MbI

3709
Stage III sporulation protein D [Bacillus
UniRef100_P15281

Bacillus subtilis

subtilis]

3710

3711
Hypothetical protein ywoH [Bacillus
UniRef100_P94578

Bacillus subtilis

YwoH

subtilis]

3712
Hypothetical protein ywoG [Bacillus
UniRef100_P94577

Bacillus subtilis

YwoG

subtilis]

3713
Hypothetical protein ywoA [Bacillus
UniRef100_P94571

Bacillus subtilis

YwoA

subtilis]

3714
Manganese-dependent inorganic
UniRef100_Q63AC7

Bacillus cereus

PpaC

pyrophosphatase [Bacillus cereus ZK]

ZK

3715
Probable manganese catalase [Bacillus
UniRef100_P80878

Bacillus subtilis

YdbD

subtilis]

3716
Hypothetical protein yqjF [Bacillus
UniRef100_P54543

Bacillus subtilis

YqjF

subtilis]

3717
BH2605 protein [Bacillus halodurans]
UniRef100_Q9K9P0

Bacillus

YhcV

halodurans

3718
Hypothetical protein yqxL [Bacillus
UniRef100_P40948

Bacillus subtilis

YqxL

subtilis]

3719
Hypothetical UPF0053 protein yqhB
UniRef100_P54505

Bacillus subtilis

YqhB

[Bacillus subtilis]

3720
Ammonium transporter nrgA [Bacillus
UniRef100_Q07429

Bacillus subtilis

NrgA

subtilis]

3721
Nitrogen regulatory PII-like protein
UniRef100_Q07428

Bacillus subtilis

NrgB

[Bacillus subtilis]

3722
ATP-dependent Clp protease
UniRef100_Q81CH1

Bacillus cereus

ClpP

proteolytic subunit [Bacillus cereus]

3723
RNA polymerase ECF-type sigma
UniRef100_Q63AF9

Bacillus cereus

SigM

factor [Bacillus cereus ZK]

ZK

3724
Hypothetical protein ywnI [Bacillus
UniRef100_P71044

Bacillus subtilis

SpoIIQ

subtilis]

3725
Hypothetical protein ywnH [Bacillus
UniRef100_P71043

Bacillus subtilis

YwnH

subtilis]

3726
Hypothetical protein ywnF [Bacillus
UniRef100_P71041

Bacillus subtilis

YwnF

subtilis]

3727
Probable cardiolipin synthetase 2
UniRef100_P71040

Bacillus subtilis

YwnE

[Bacillus subtilis]

3728
UPI00003CB764 UniRef100 entry
UniRef100_UPI00003CB764

YhcA

3729
Antibiotic/siderophore biosynthesis
UniRef100_Q81DP7

Bacillus cereus

protein [Bacillus cereus]

3730
Probable serine activating enzyme
UniRef100_P45745

Bacillus subtilis

DhbF

[Bacillus subtilis]

3731
Isochorismatase [Bacillus subtilis]
UniRef100_P45743

Bacillus subtilis

DhbB

3732
2,3-dihydroxybenzoate-AMP ligase
UniRef100_P40871

Bacillus subtilis

DhbE

[Bacillus subtilis]

3733
Isochorismate synthase dhbC [Bacillus
UniRef100_P45744

Bacillus subtilis

DhbC

subtilis]

3734
2,3-dihydro-2,3-dihydroxybenzoate
UniRef100_P39071

Bacillus subtilis

DhbA

dehydrogenase [Bacillus subtilis]

3735
YuiI protein [Bacillus subtilis]
UniRef100_O32102

Bacillus subtilis

YuiI

3736
Iron-uptake system permease protein
UniRef100_P40411

Bacillus subtilis

FeuC

feuC [Bacillus subtilis]

3737
Iron-uptake system permease protein
UniRef100_P40410

Bacillus subtilis

FeuB

feuB [Bacillus subtilis]

3738
Iron-uptake system binding protein
UniRef100_P40409

Bacillus subtilis

FeuA

precursor [Bacillus subtilis]

3739
Putative HTH-type transcriptional
UniRef100_P40408

Bacillus subtilis

YbbB

regulator ybbB [Bacillus subtilis]

3740
Hypothetical protein ywnG [Bacillus
UniRef100_P71042

Bacillus subtilis

YwnC

subtilis]

3741
UPI00003CC069 UniRef100 entry
UniRef100_UPI00003CC069

3742
IS1627s1-related, transposase [Bacillus
UniRef100_Q7CMD0

Bacillus anthracis str. A2012

anthracis str. A2012]

3743
Hypothetical protein ywnB [Bacillus
UniRef100_P71037

Bacillus subtilis

YwnB

subtilis]

3744
Hypothetical protein ywnA [Bacillus
UniRef100_P71036

Bacillus subtilis

YwnA

subtilis]

3745

3746
Hypothetical protein ywmF [Bacillus
UniRef100_P70963

Bacillus subtilis

YwmF

subtilis]

3747

MoaA

3748
FdhD protein homolog [Bacillus subtilis]
UniRef100_P39756

Bacillus subtilis

FdhD

3749
BH2528 protein [Bacillus halodurans]
UniRef100_Q9K9W7

Bacillus

YbfB

halodurans

3750
Formate dehydrogenase alpha chain
UniRef100_Q9K9W5

Bacillus

YjgC

[Bacillus halodurans]

halodurans

3751

YwmD

3752
Hypothetical protein ywmC precursor
UniRef100_P70960

Bacillus subtilis

YwmC

[Bacillus subtilis]

3753
Stage II sporulation protein D [Bacillus
UniRef100_P07372

Bacillus subtilis

SpoIID

subtilis]

3754
UDP-N-acetylglucosamine 1-
UniRef100_P70965

Bacillus subtilis

MurAA

carboxyvinyltransferase 1 [Bacillus

subtilis]

3755
YwmB protein [Bacillus subtilis]
UniRef100_O32277

Bacillus subtilis

YwmB

3756
YwzB protein [Bacillus subtilis]
UniRef100_O32278

Bacillus subtilis

3757
ATP synthase epsilon chain [Bacillus
UniRef100_P37812

Bacillus subtilis

AtpC

subtilis]

3758
ATP synthase beta chain [Bacillus
UniRef100_P37809

Bacillus subtilis

AtpD

subtilis]

3759
ATP synthase gamma chain [Bacillus
UniRef100_P37810

Bacillus subtilis

AtpG

subtilis]

3760
ATP synthase alpha chain [Bacillus
UniRef100_Q659H2

Bacillus

AtpA

amyloliquefaciens]

amyloliquefaciens

3761
ATP synthase delta chain [Bacillus
UniRef100_P37811

Bacillus subtilis

AtpH

subtilis]

3762
ATP synthase B chain [Bacillus subtilis]
UniRef100_P37814

Bacillus subtilis

AtpF

3763
ATP synthase a chain [Bacillus subtilis]
UniRef100_P37813

Bacillus subtilis

AtpB

3764

AtpI

3765
Uracil phosphoribosyltransferase
UniRef100_P39149

Bacillus subtilis

Upp

[Bacillus subtilis]

3766
Serine hydroxymethyltransferase
UniRef100_P39148

Bacillus subtilis

GlyA

[Bacillus subtilis]

3767
Hypothetical protein ywlG [Bacillus
UniRef100_P39157

Bacillus subtilis

YwlG

subtilis]

3768
Hypothetical lacA/rpiB family protein
UniRef100_P39156

Bacillus subtilis

YwlF

ywlF [Bacillus subtilis]

3769
Putative low molecular weight protein-
UniRef100_P39155

Bacillus subtilis

YwlE

tyrosine-phosphatase ywlE [Bacillus

subtilis]

3770
Hypothetical UPF0059 protein ywlD
UniRef100_P39154

Bacillus subtilis

YwlD

[Bacillus subtilis]

3771
Hypothetical protein ywlC [Bacillus
UniRef100_P39153

Bacillus subtilis

YwlC

subtilis]

3772
Hypothetical protein ywlB [Bacillus
UniRef100_P39152

Bacillus subtilis

YwlB

subtilis]

3773
Stage II sporulation protein R [Bacillus
UniRef100_P39151

Bacillus subtilis

SpoIIR

subtilis]

3774
Hypothetical protein yhjI [Bacillus
UniRef100_O07563

Bacillus subtilis

GlcP

subtilis]

3775
Hypothetical protein yhjJ [Bacillus
UniRef100_O07564

Bacillus subtilis

YhjJ

subtilis]

3776
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81NC8

Bacillus

YhjK

anthracis

3777
Hypothetical protein yhjL [Bacillus
UniRef100_O07566

Bacillus subtilis

YhjL

subtilis]

3778
Hypothetical protein yhjM [Bacillus
UniRef100_O07567

Bacillus subtilis

YhjM

subtilis]

3779
HemK protein homolog [Bacillus
UniRef100_P45873

Bacillus subtilis

YwkE

subtilis]

3780
Peptide chain release factor 1 [Bacillus
UniRef100_P45872

Bacillus subtilis

PrfA

subtilis]

3781
Hypothetical protein ywkD [Bacillus
UniRef100_P45871

Bacillus subtilis

YwkD

subtilis]

3782
Hypothetical protein ywkC [Bacillus
UniRef100_P45870

Bacillus subtilis

YwkC

subtilis]

3783
Hypothetical protein ywkB [Bacillus
UniRef100_P45869

Bacillus subtilis

YwkB

subtilis]

3784
Probable NAD-dependent malic
UniRef100_P45868

Bacillus subtilis

YwkA

enzyme 2 [Bacillus subtilis]

3785
Thymidine kinase [Bacillus subtilis]
UniRef100_Q03221

Bacillus subtilis

Tdk

3786
50S ribosomal protein L31 [Bacillus
UniRef100_Q03223

Bacillus subtilis

subtilis]

3787
Transcription termination factor rho
UniRef100_Q03222

Bacillus subtilis

Rho

[Bacillus subtilis]

3788
Hypothetical protein ywjI [Bacillus
UniRef100_Q03224

Bacillus subtilis

YwjI

subtilis]

3789
UDP-N-acetylglucosamine 1-
UniRef100_P19670

Bacillus subtilis

MurAB

carboxyvinyltransferase 2 [Bacillus

subtilis]

3790
Transaldolase [Bacillus subtilis]
UniRef100_P19669

Bacillus subtilis

YwjH

3791
Probable fructose-bisphosphate
UniRef100_P13243

Bacillus subtilis

FbaA

aldolase 1 [Bacillus subtilis]

3792
Sporulation initiation
UniRef100_P06628

Bacillus subtilis

Spo0F

phosphotransferase F [Bacillus subtilis]

3793
Hypothetical protein ywjG [Bacillus
UniRef100_P06629

Bacillus subtilis

YwjG

subtilis]

3794

PyrG

3795
DNA-directed RNA polymerase delta
UniRef100_P12464

Bacillus subtilis

RpoE

subunit [Bacillus subtilis]

3796
Acyl-CoA dehydrogenase [Bacillus
UniRef100_Q814S9

Bacillus cereus

AcdA

cereus]

3797
Acyl-CoA dehydrogenase [Bacillus
UniRef100_Q81JV7

Bacillus

MmgC

anthracis]

anthracis

3798
UPI00003CC303 UniRef100 entry
UniRef100_UPI00003CC303

MmgB

3799
Acetyl-CoA acetyltransferase
UniRef100_Q8EM47

Oceanobacillus

MmgA

[Oceanobacillus iheyensis]

iheyensis

3800
Hypothetical protein ywjF [Bacillus
UniRef100_P45866

Bacillus subtilis

YwjF

subtilis]

3801

YwjE

3802
Hypothetical protein ywjC [Bacillus
UniRef100_P45863

Bacillus subtilis

subtilis]

3803
Arginyl-tRNA synthetase [Bacillus
UniRef100_P46906

Bacillus subtilis

ArgS

subtilis]

3804

YwiB

3805
Agmatinase [Bacillus subtilis]
UniRef100_P70999

Bacillus subtilis

SpeB

3806
Spermidine synthase [Bacillus subtilis]
UniRef100_P70998

Bacillus subtilis

SpeE

3807
UPI00002A1DD1 UniRef100 entry
UniRef100_UPI00002A1DD1

3808
Hypothetical protein ywhE [Bacillus
UniRef100_P70997

Bacillus subtilis

YwhE

subtilis]

3809
Hypothetical protein ywhD [Bacillus
UniRef100_P70996

Bacillus subtilis

YwhD

subtilis]

3810
Hypothetical protein ywhC [Bacillus
UniRef100_P70995

Bacillus subtilis

YwhC

subtilis]

3811
Probable tautomerase ywhB [Bacillus
UniRef100_P70994

Bacillus subtilis

subtilis]

3812
Conserved protein [Methanosarcina
UniRef100_Q8PUC6

Methanosarcina mazei

mazei]

3813
Hypothetical protein ywgA [Bacillus
UniRef100_P71046

Bacillus subtilis

YwgA

subtilis]

3814
Hypothetical protein ywfO [Bacillus
UniRef100_P39651

Bacillus subtilis

YwfO

subtilis]

3815
YwzC protein [Bacillus subtilis]
UniRef100_O32280

Bacillus subtilis

3816
Prespore specific transcriptional
UniRef100_P39650

Bacillus subtilis

RsfA

regulator rsfA [Bacillus subtilis]

3817
Hypothetical protein yjdJ precursor
UniRef100_O31651

Bacillus subtilis

[Bacillus subtilis]

3818
Hypothetical protein ywfL [Bacillus
UniRef100_P39648

Bacillus subtilis

YwfL

subtilis]

3819
4-hydroxybenzoate 3-monooxygenase
UniRef100_Q59724

Pseudomonas sp
YhjG

[Pseudomonas sp]

3820
Benzoate transporter protein
UniRef100_Q6XUN5

Pseudomonas

YceI

[Pseudomonas sp. ND6]

sp. ND6

3821
Putative 3,4-dihydroxyphenylacetate
UniRef100_Q6N986

Rhodopseudomonas palustris

2,3-dioxygenase [Rhodopseudomonas

palustris]

3822
4-oxalocrotonate tautomerase
UniRef100_Q9Z431

Pseudomonas putida

[Pseudomonas putida]

3823
4-oxalocrotonate decarboxylase
UniRef100_Q84I96

Pseudomonas sp. S-47

[Pseudomonas sp. S-47]

3824

DhaS

3825
Putative transcriptional regulator
UniRef100_Q9F7C7

Agrobacterium

KipR

[Agrobacterium tumefaciens]

tumefaciens

3826
Hypothetical protein SCO6305
UniRef100_Q93RT6

Streptomyces coelicolor

[Streptomyces coelicolor]

3827
Phosphate acetyltransferase [Bacillus
UniRef100_P39646

Bacillus subtilis

Pta

subtilis]

3828
Hypothetical protein ywfI [Bacillus
UniRef100_P39645

Bacillus subtilis

YwfI

subtilis]

3829

YuaB

3830
Na+-transporting ATP synthase
UniRef100_Q9KF87

Bacillus

YubG

[Bacillus halodurans]

halodurans

3831
Hypothetical protein ywdL [Bacillus
UniRef100_P39620

Bacillus subtilis

YwdL

subtilis]

3832
Multidrug ABC transporter, permease
UniRef100_Q63D13

Bacillus cereus

YfiN

[Bacillus cereus ZK]

ZK

3833
ABC transporter, permease protein
UniRef100_Q73AB4

Bacillus cereus

YfiM

[Bacillus cereus]

3834
Multidrug ABC transporter, ATP-binding
UniRef100_Q63D15

Bacillus cereus

YfiL

protein [Bacillus cereus ZK]

ZK

3835
Sensor histidine kinase, putative
UniRef100_Q73AB6

Bacillus cereus

YfiJ

[Bacillus cereus]

3836
DNA-binding response regulator, LuxR
UniRef100_Q73AB7

Bacillus cereus

YfiK

family [Bacillus cereus]

3837

YwdK

3838
Putative purine permease ywdJ
UniRef100_P39618

Bacillus subtilis

YwdJ

[Bacillus subtilis]

3839
Hypothetical protein ywdI [Bacillus
UniRef100_P39617

Bacillus subtilis

YwdI

subtilis]

3840
Probable aldehyde dehydrogenase
UniRef100_P39616

Bacillus subtilis

YwdH

ywdH [Bacillus subtilis]

3841
Uracil-DNA glycosylase [Bacillus
UniRef100_P39615

Bacillus subtilis

Ung

subtilis]

3842

YwdF

3843
Phosphomethylpyrimidine kinase
UniRef100_P39610

Bacillus subtilis

ThiD

[Bacillus subtilis]

3844
Hypothetical protein ywdA [Bacillus
UniRef100_P39609

Bacillus subtilis

subtilis]

3845
Sucrose-6-phosphate hydrolase
UniRef100_P07819

Bacillus subtilis

SacA

[Bacillus subtilis]

3846
PTS system, sucrose-specific IIBC
UniRef100_P05306

Bacillus subtilis

SacP

component [Bacillus subtilis]

3847

SacT

3848

3849
Minor extracellular protease vpr
UniRef100_P29141

Bacillus subtilis

Vpr

precursor [Bacillus subtilis]

3850

3851

YwcH

3852
Nitro/flavin reductase [Bacillus subtilis]
UniRef100_P39605

Bacillus subtilis

NfrA

3853
N-acyl-L-amino acid amidohydrolase
UniRef100_Q9KCF8

Bacillus

YhaA

[Bacillus halodurans]

halodurans

3854
Hypothetical protein ywcF [Bacillus
UniRef100_P39604

Bacillus subtilis

RodA

subtilis]

3855

RodA

3856
Membrane protein [Clostridium tetani]
UniRef100_Q897A1

Clostridium tetani

3857
Hypothetical protein ywcE precursor
UniRef100_P39603

Bacillus subtilis

[Bacillus subtilis]

3858
Clostripain [Methanosarcina
UniRef100_Q8TIY6

Methanosarcina acetivorans

acetivorans]

3859
YfmK protein [Bacillus subtilis]
UniRef100_O34536

Bacillus subtilis

YfmK

3860
ABC transporter, ATP-
UniRef100_Q882Y2

Pseudomonas

YgaD

binding/permease protein

syringae

[Pseudomonas syringae]

3861
HlyB/MsbA family ABC transporter
UniRef100_Q7NIB9

Gloeobacter

YwjA

[Gloeobacter violaceus]

violaceus

3862

3863

3864

3865

3866
Response regulator [Bacillus cereus
UniRef100_Q63FE4

Bacillus cereus

ComA

ZK]

ZK

3867

3868
Quinol oxidase polypeptide IV [Bacillus
UniRef100_P34959

Bacillus subtilis

QoxD

subtilis]

3869
Quinol oxidase polypeptide III (EC
UniRef100_P34958

Bacillus subtilis

QoxC

1.10.3.—) (Quinol oxidase aa3-600,

subunit qoxC) (Oxidase aa(3)-600

subunit 3) [Bacillus subtilis]

3870
Quinol oxidase polypeptide I (EC
UniRef100_P34956

Bacillus subtilis

QoxB

1.10.3.—) (Quinol oxidase aa3-600,

subunit qoxB) (Oxidase aa(3)-600

subunit 1) [Bacillus subtilis]

3871

QoxA

3872
UDP-glucose 4-epimerase
UniRef100_Q8R8R8

Thermoanaerobacter

GalE

[Thermoanaerobacter tengcongensis]

tengcongensis

3873

3874

3875

3876
Hypothetical protein ywqN [Bacillus
UniRef100_P96726

Bacillus subtilis

YwqN

subtilis]

3877
Hypothetical protein ywcB [Bacillus
UniRef100_P39600

Bacillus subtilis

subtilis]

3878
Putative symporter ywcA [Bacillus
UniRef100_P39599

Bacillus subtilis

YwcA

subtilis]

3879

XylB

3880
Xylose isomerase [Bacillus sp.]
UniRef100_P54272

Bacillus sp.
XylA

3881

XylR

3882
Thiamine-phosphate
UniRef100_P39594

Bacillus subtilis

ThiE

pyrophosphorylase [Bacillus subtilis]

3883
Hydroxyethylthiazole kinase [Bacillus
UniRef100_P39593

Bacillus subtilis

ThiM

subtilis]

3884
Putative HTH-type transcriptional
UniRef100_P39592

Bacillus subtilis

YwbI

regulator ywbI [Bacillus subtilis]

3885
Putative HTH-type transcriptional
UniRef100_P39592

Bacillus subtilis

YwbI

regulator ywbI [Bacillus subtilis]

3886

3887

YwqH

3888
Holin-like protein cidA [Bacillus subtilis]
UniRef100_P39591

Bacillus subtilis

YwbH

3889
Hypothetical protein ywbG [Bacillus
UniRef100_P39590

Bacillus subtilis

YwbG

subtilis]

3890
HlyC domain protein [Bacillus cereus]
UniRef100_Q731T5

Bacillus cereus

3891

OpuE

3892
Hypothetical UPF0064 protein ywbD
UniRef100_P39587

Bacillus subtilis

YwbD

[Bacillus subtilis]

3893
Hypothetical protein ywbC [Bacillus
UniRef100_P39586

Bacillus subtilis

YwbC

subtilis]

3894
Phosphoglycerate dehydrogenase
UniRef100_Q8GCC8

Bacillus

YvcT

[Bacillus coagulans]

coagulans

3895
Glycerate dehydrogenase
UniRef100_Q8EP33

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

3896
General stress protein A [Bacillus
UniRef100_P25148

Bacillus subtilis

GspA

subtilis]

3897
YdfH protein [Bacillus subtilis]
UniRef100_P96685

Bacillus subtilis

YdfH

3898
YdfI protein [Bacillus subtilis]
UniRef100_P96686

Bacillus subtilis

YdfI

3899
YdfJ protein [Bacillus subtilis]
UniRef100_P96687

Bacillus subtilis

YdfJ

3900
Hypothetical protein ywaF [Bacillus
UniRef100_P25149

Bacillus subtilis

YwaF

subtilis]

3901
MFS transporter, phthalate permease
UniRef100_Q884B5

Pseudomonas

YcbE

family [Pseudomonas syringae]

syringae

3902
Hypothetical protein OB2215
UniRef100_Q8EP98

Oceanobacillus

YitF

[Oceanobacillus iheyensis]

iheyensis

3903
2-dehydro-3-deoxyphosphogluconate
UniRef100_Q8EP99

Oceanobacillus

KdgA

aldolase [Oceanobacillus iheyensis]

iheyensis

3904
2-keto-3-deoxygluconate kinase
UniRef100_Q8EPA0

Oceanobacillus

KdgK

[Oceanobacillus iheyensis]

iheyensis

3905
Transcriptional regulator
UniRef100_Q8EPA1

Oceanobacillus

KipR

[Oceanobacillus iheyensis]

iheyensis

3906

YwaD

3907
Hypothetical protein ywoD [Bacillus
UniRef100_P94574

Bacillus subtilis

YwoD

subtilis]

3908

3909
Hypothetical protein ywaC [Bacillus
UniRef100_P39583

Bacillus subtilis

YwaC

subtilis]

3910

3911

DltA

3912
Protein dltB [Bacillus subtilis]
UniRef100_P39580

Bacillus subtilis

DltB

3913
D-alanine--poly(phosphoribitol) ligase
UniRef100_P39579

Bacillus subtilis

subunit 2 [Bacillus subtilis]

3914
Protein dltD precursor [Bacillus subtilis]
UniRef100_P39578

Bacillus subtilis

DltD

3915
Putative branched-chain-amino-acid
UniRef100_P39576

Bacillus subtilis

YwaA

aminotransferase [Bacillus subtilis]

3916
Putative macrolide-efflux determinant,
UniRef100_O32203

Bacillus subtilis

YvqJ

YvgJ [Bacillus subtilis]

3917
Probable 6-phospho-beta-glucosidase
UniRef100_P46320

Bacillus subtilis

LicH

[Bacillus subtilis]

3918
PTS system, lichenan-specific IIA
UniRef100_P46319

Bacillus subtilis

LicA

component [Bacillus subtilis]

3919
PTS system, lichenan-specific IIC
UniRef100_P46317

Bacillus subtilis

LicC

component [Bacillus subtilis]

3920
PTS system, lichenan-specific IIB
UniRef100_P46318

Bacillus subtilis

component [Bacillus subtilis]

3921
Probable licABCH operon regulator
UniRef100_P46321

Bacillus subtilis

LicR

[Bacillus subtilis]

3922

3923
Putative 3-methyladenine DNA
UniRef100_P94378

Bacillus subtilis

YxlJ

glycosylase [Bacillus subtilis]

3924
Transcriptional regulator [Rhizobium
UniRef100_Q988D9

Rhizobium loti

YdhC

loti]

3925
Citrate synthase III [Bacillus subtilis]
UniRef100_P45858

Bacillus subtilis

MmgD

3926
2-methylisocitrate dehydratase [Bacillus
UniRef100_Q6HJ23

Bacillus

MmgE

thuringiensis]

thuringiensis

3927
Putative carboxyvinyl-
UniRef100_P54528

Bacillus subtilis

YqiQ

carboxyphosphonate phosphorylmutase

[Bacillus subtilis]

3928
Chromate transporter [Bacillus
UniRef100_Q6HIU6

Bacillus

YwrA

thuringiensis]

thuringiensis

3929
BH0575 protein [Bacillus halodurans]
UniRef100_Q9KFB0

Bacillus halodurans

3930

YwjA

3931
YesT protein [Bacillus subtilis]
UniRef100_O31523

Bacillus subtilis

YesT

3932

YweA

3933

YweA

3934
Drug resistance transporter Bcr/CflA
UniRef100_Q6HPJ5

Bacillus

YdgK

subfamily; possible bicyclomycin

thuringiensis

resistance protein [Bacillus

thuringiensis]

3935
YxlG protein [Bacillus subtilis]
UniRef100_P94375

Bacillus subtilis

YxlG

3936
YxlF protein [Bacillus subtilis]
UniRef100_P94374

Bacillus subtilis

YxlF

3937
YxlE protein [Bacillus subtilis]
UniRef100_P94373

Bacillus subtilis

3938

3939
Hypothetical protein yxlC [Bacillus
UniRef100_P94371

Bacillus subtilis

subtilis]

3940
RNA polymerase sigma factor sigY
UniRef100_P94370

Bacillus subtilis

SigY

[Bacillus subtilis]

3941
YxlH protein [Bacillus subtilis]
UniRef100_P94376

Bacillus subtilis

YxlH

3942
Catalase [Bacillus halodurans]
UniRef100_Q9KDA8

Bacillus

KatX

halodurans

3943
Catalase [Bacillus subtilis]
UniRef100_Q9AQQ9

Bacillus subtilis

KatA

3944
Peroxide operon transcriptional
UniRef100_Q71YY4

Listeria

PerR

regulator [Listeria monocytogenes]

monocytogenes

3945
Ferrochelatase [Oceanobacillus
UniRef100_Q8ERX9

Oceanobacillus

HemH

iheyensis]

iheyensis

3946
Hypothetical protein yhcI [Bacillus
UniRef100_P54593

Bacillus subtilis

YhcI

subtilis]

3947
Bacitracin transport ATP-binding
UniRef100_Q81AB3

Bacillus cereus

YhcH

protein bcrA [Bacillus cereus]

3948
MrsE protein [Bacillus sp.]
UniRef100_Q9RC25

Bacillus sp.

3949

3950
ABC-2 type transport system ATP-
UniRef100_Q8ZZG9

Pyrobaculum

YdbJ

binding protein [Pyrobaculum

aerophilum

aerophilum]

3951

3952
Transcriptional regulator [Bacillus
UniRef100_Q9KFL9

Bacillus halodurans

halodurans]

3953

3954
ClyA protein [Plasmid pAD1]
UniRef100_Q52055
Plasmid pAD1
IspA

3955
Lantibiotic mersacidin transporter
UniRef100_Q9KFM8

Bacillus

YgaD

system [Bacillus halodurans]

halodurans

3956

3957
Lantibiotic mersacidin [Bacillus
UniRef100_Q9KFM5

Bacillus halodurans

halodurans]

3958
Lantibiotic mersacidin modifying
UniRef100_Q9KFM4

Bacillus halodurans

enzyme [Bacillus halodurans]

3959
Pectate lyase Pel-34K [Bacillus sp. P-
UniRef100_Q8L0S6

Bacillus sp. P-
Pel

2850]

2850

3960
Hypothetical UPF0031 protein yxkO
UniRef100_P94368

Bacillus subtilis

YxkO

[Bacillus subtilis]

3961
Transport ATP-binding protein cydD
UniRef100_P94367

Bacillus subtilis

CydD

[Bacillus subtilis]

3962
Transport ATP-binding protein cydC
UniRef100_P94366

Bacillus subtilis

CydC

[Bacillus subtilis]

3963
Cytochrome d ubiquinol oxidase
UniRef100_P94365

Bacillus subtilis

CydB

subunit II [Bacillus subtilis]

3964
Cytochrome d ubiquinol oxidase
UniRef100_P94364

Bacillus subtilis

CydA

subunit I [Bacillus subtilis]

3965
Hypothetical transport protein ywcJ
UniRef100_P39608

Bacillus subtilis

YwcJ

[Bacillus subtilis]

3966
Hypothetical protein [Bacillus cereus]
UniRef100_Q735J7

Bacillus cereus

3967
Probable NAD-dependent malic
UniRef100_P54572

Bacillus subtilis

MleA

enzyme 1 [Bacillus subtilis]

3968
Malate-2H(+)/Na(+)-lactate antiporter
UniRef100_P54571

Bacillus subtilis

MleN

[Bacillus subtilis]

3969

AnsB

3970
L-asparaginase [Bacillus subtilis]
UniRef100_P26900

Bacillus subtilis

AnsA

3971

AnsR

3972
Hypothetical protein ykgB [Bacillus
UniRef100_O34499

Bacillus subtilis

YkgB

subtilis]

3973
Hypothetical sensory transduction
UniRef100_P42421

Bacillus subtilis

YxdJ

protein yxdJ [Bacillus subtilis]

3974
Hypothetical sensor-like histidine
UniRef100_P42422

Bacillus subtilis

YxdK

kinase yxdK [Bacillus subtilis]

3975
Hypothetical ABC transporter ATP-
UniRef100_P42423

Bacillus subtilis

YxdL

binding protein yxdL [Bacillus subtilis]

3976
Hypothetical protein yxdM [Bacillus
UniRef100_P42424

Bacillus subtilis

YxdM

subtilis]

3977
Hypothetical protein yxeA precursor
UniRef100_P54940

Bacillus subtilis

YxeA

[Bacillus subtilis]

3978
YxkH protein [Bacillus subtilis]
UniRef100_P94361

Bacillus subtilis

YxkH

3979
Transcriptional regulator [Bacillus
UniRef100_Q9KFA7

Bacillus

YdeL

halodurans]

halodurans

3980
Hypothetical protein orfD [Lactococcus
UniRef100_O86288

Lactococcus

YdaM

lactis]

lactis

3981
Citrate/malate transporter [Bacillus
UniRef100_P94363

Bacillus subtilis

YxkJ

subtilis]

3982
Glycerophosphodiester
UniRef100_Q8EMK9

Oceanobacillus

YhdW

phosphodiesterase [Oceanobacillus

iheyensis

iheyensis]

3983
Hypothetical protein yhjA precursor
UniRef100_O07555

Bacillus subtilis

[Bacillus subtilis]

3984

3985
YfnI [Bacillus subtilis]
UniRef100_O06487

Bacillus subtilis

YfnI

3986
Hypothetical lipoprotein yfjD precursor
UniRef100_O31555

Bacillus subtilis

YfjD

[Bacillus subtilis]

3987
YxkD protein [Bacillus subtilis]
UniRef100_P94357

Bacillus subtilis

YxkD

3988
Arginine repressor, argR [Bacillus
UniRef100_Q81II2

Bacillus cereus

AhrC

cereus]

3989
Arginine deiminase [Bacillus cereus]
UniRef100_Q73E87

Bacillus cereus

YkgA

3990
Ornithine carbamoyltransferase
UniRef100_Q73E86

Bacillus cereus

ArgF

[Bacillus cereus]

3991
Arginine/ornithine antiporter [Bacillus
UniRef100_Q73E85

Bacillus cereus

YvsH

cereus]

3992
Carbamate kinase [Bacillus cereus]
UniRef100_Q81IH8

Bacillus cereus

3993
Transcriptional regulator, Crp family
UniRef100_Q6HP25

Bacillus

Fnr

[Bacillus thuringiensis]

thuringiensis

3994
Beta-glucosidase [Oceanobacillus
UniRef100_Q8ES64

Oceanobacillus

YdhP

iheyensis]

iheyensis

3995
PTS system, lichenan-specific IIc
UniRef100_Q6D101

Erwinia

LicC

component [Erwinia carotovora]

carotovora

3996
Hypothetical protein [Bacillus
UniRef100_Q6HC13

Bacillus thuringiensis

thuringiensis]

3997

SigV

3998
Anaerobic ribonucleoside-triphosphate
UniRef100_O26387

Methanobacterium

reductase activating protein

thermoautotrophicum

[Methanobacterium

thermoautotrophicum]

3999
Hypothetical protein yxeC [Bacillus
UniRef100_P54942

Bacillus subtilis

subtilis]

4000

4001

4002
Hypothetical protein [Anabaena sp.]
UniRef100_Q9AIM4

Anabaena sp.
FtsH

4003
Peptidase T [Bacillus subtilis]
UniRef100_P55179

Bacillus subtilis

PepT

4004
Beta-fructosidase FruA [Bacillus
UniRef100_Q8GM36

Bacillus

SacA

megaterium]

megaterium

4005
Sugar transporter FruP [Bacillus
UniRef100_Q8GM37

Bacillus

YwbF

megaterium]

megaterium

4006
ABC transporter membrane-spanning
UniRef100_Q8DNN9

Streptococcus

YesQ

permease-sugar transport

pneumoniae

[Streptococcus pneumoniae]

4007
ABC transporter membrane-spanning
UniRef100_Q8DNN8

Streptococcus

YurN

permease-sugar transporter

pneumoniae

[Streptococcus pneumoniae]

4008

YesO

4009
Repressor FruR [Bacillus megaterium]
UniRef100_Q8GM38

Bacillus

RbsR

megaterium

4010
Hypothetical protein [Bacillus cereus]
UniRef100_Q738I8

Bacillus cereus

4011

YjeA

4012
Hypothetical protein yxjG [Bacillus
UniRef100_P42318

Bacillus subtilis

YxjG

subtilis]

4013
YkcB protein [Bacillus subtilis]
UniRef100_O34575

Bacillus subtilis

YkcB

4014
Putative glycosyl transferase ykcC
UniRef100_O34319

Bacillus subtilis

YkcC

[Bacillus subtilis]

4015

GalE

4016

4017

4018

4019
Hypothetical 10.1 kDa protein [Bacillus
UniRef100_O50571

Bacillus pseudofirmus

pseudofirmus]

4020

4021
Hypothetical transport protein yxjA
UniRef100_P42312

Bacillus subtilis

YxjA

[Bacillus subtilis]

4022
Catalase 2 [Bacillus subtilis]
UniRef100_P42234

Bacillus subtilis

KatE

4023
Catalase 2 [Bacillus subtilis]
UniRef100_P42234

Bacillus subtilis

KatE

4024
DNA gyrase inhibitory protein, GyrI
UniRef100_Q97DI7

Clostridium

YosT

[Clostridium acetobutylicum]

acetobutylicum

4025
Probable glycosyl hydrolase [Erwinia
UniRef100_Q6D774

Erwinia

YckE

carotovora]

carotovora

4026
PTS system, beta-glucoside-specific
UniRef100_Q9KG19

Bacillus

BglP

enzyme II, ABC component [Bacillus

halodurans

halodurans]

4027
Transcription antiterminator licT
UniRef100_P39805

Bacillus subtilis

LicT

[Bacillus subtilis]

4028
Hypothetical conserved protein
UniRef100_Q8ES40

Oceanobacillus

YkoC

[Oceanobacillus iheyensis]

iheyensis

4029
Cation ABC transporter ATP-binding
UniRef100_Q8ES39

Oceanobacillus

YkoD

protein [Oceanobacillus iheyensis]

iheyensis

4030
Hypothetical conserved protein
UniRef100_Q8ES38

Oceanobacillus

YkoE

[Oceanobacillus iheyensis]

iheyensis

4031
Transcriptional activator of extracellular
UniRef100_Q8ESZ0

Oceanobacillus

TenA

enzyme genes [Oceanobacillus

iheyensis

iheyensis]

4032
Isocitrate lyase [Bacillus cereus]
UniRef100_Q73C38

Bacillus cereus

YqiQ

4033
Malate synthase A [Bacillus cereus]
UniRef100_Q73C39

Bacillus cereus

4034

4035
Hypothetical protein yycA [Bacillus
UniRef100_P37483

Bacillus subtilis

YycA

subtilis]

4036
Bacitracin export permease protein
UniRef100_O34741

Bacillus subtilis

YtsD

bceB [Bacillus subtilis]

4037
Bacitracin export ATP-binding protein
UniRef100_O34697

Bacillus subtilis

YtsC

bceA [Bacillus subtilis]

4038
Sensor protein bceS [Bacillus subtilis]
UniRef100_O35044

Bacillus subtilis

YtsB

4039
Sensory transduction protein bceR
UniRef100_O34951

Bacillus subtilis

YtsA

[Bacillus subtilis]

4040
Beta-glucosidase [Bacillus subtilis]
UniRef100_P40740

Bacillus subtilis

BglH

4041
PTS system, beta-glucoside-specific
UniRef100_P40739

Bacillus subtilis

BglP

IIABC component [Bacillus subtilis]

4042
Hypothetical protein yxeG [Bacillus
UniRef100_P54946

Bacillus subtilis

YxeG

subtilis]

4043
Hypothetical protein yxeI [Bacillus
UniRef100_P54948

Bacillus subtilis

YxeI

subtilis]

4044

4045

4046
Hypothetical protein yxiA precursor
UniRef100_P42293

Bacillus subtilis

YxiA

[Bacillus subtilis]

4047
ATP-dependent RNA helicase dbpA
UniRef100_P42305

Bacillus subtilis

DeaD

[Bacillus subtilis]

4048
Hypothetical protein OB2811
UniRef100_Q8EMN1

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

4049
Hypothetical protein OB2810
UniRef100_Q8EMN2

Oceanobacillus iheyensis

[Oceanobacillus iheyensis]

4050

YxiD

4051

4052
BH4015 protein [Bacillus halodurans]
UniRef100_Q9K5S3

Bacillus

YxiB

halodurans

4053
Pyrimidine-nucleoside phosphorylase
UniRef100_P39142

Bacillus subtilis

Pdp

[Bacillus subtilis]

4054
Pyrimidine nucleoside transport protein
UniRef100_P39141

Bacillus subtilis

NupC

[Bacillus subtilis]

4055
Deoxyribose-phosphate aldolase
UniRef100_P39121

Bacillus subtilis

Dra

[Bacillus subtilis]

4056
Deoxyribonucleoside regulator [Bacillus
UniRef100_P39140

Bacillus subtilis

DeoR

subtilis]

4057
Hypothetical protein [Bacillus anthracis]
UniRef100_Q81X49

Bacillus anthracis

4058
YdaJ protein [Bacillus subtilis]
UniRef100_P96584

Bacillus subtilis

YdaJ

4059
YdaK protein [Bacillus subtilis]
UniRef100_P96585

Bacillus subtilis

YdaK

4060
YdaL protein [Bacillus subtilis]
UniRef100_O31487

Bacillus subtilis

YdaL

4061
YdaM protein [Bacillus subtilis]
UniRef100_P96587

Bacillus subtilis

YdaM

4062
YdaN protein [Bacillus subtilis]
UniRef100_O31488

Bacillus subtilis

YdaN

4063
Valine-pyruvate aminotransferase
UniRef100_Q8YTB2

Anabaena sp.
AlaT

[Anabaena sp.]

4064
Hypothetical protein [Leifsonia xyli]
UniRef100_Q6AEW0

Leifsonia xyli

4065
Hypothetical protein [Leifsonia xyli]
UniRef100_Q6AEW1

Leifsonia xyli

YwfB

4066
Similarities with putative carboxylase
UniRef100_Q7NA29

Photorhabdus

YwfE

[Photorhabdus luminescens]

luminescens

4067
Transporter, Drug/Metabolite Exporter
UniRef100_Q63AK7

Bacillus cereus

YoaV

family [Bacillus cereus ZK]

ZK

4068
Probable fructose-bisphosphate
UniRef100_P42420

Bacillus subtilis

FbaB

aldolase 2 [Bacillus subtilis]

4069
IolI protein [Bacillus subtilis]
UniRef100_P42419

Bacillus subtilis

IolI

4070
IolH protein [Bacillus subtilis]
UniRef100_P42418

Bacillus subtilis

IolH

4071
Myo-inositol dehydrogenase [Bacillus
UniRef100_Q6B6R7

Bacillus subtilis

Idh

subtilis]

4072
Myo-inositol transport protein [Bacillus
UniRef100_P42417

Bacillus subtilis

IolF

subtilis]

4073
IolE protein [Bacillus subtilis]
UniRef100_P42416

Bacillus subtilis

IolE

4074
Probable malonic semialdehyde
UniRef100_P42415

Bacillus subtilis

IolD

oxidative decarboxylase [Bacillus

subtilis]

4075
Protein iolC [Bacillus subtilis]
UniRef100_P42414

Bacillus subtilis

IolC

4076
IolB protein [Bacillus subtilis]
UniRef100_P42413

Bacillus subtilis

IolB

4077
Probable methylmalonate-
UniRef100_P42412
acylating
MmsA

semialdehyde dehydrogenase

[acylating] [Bacillus subtilis]

4078
DNA-binding protein iolR [Bacillus
UniRef100_P46337

Bacillus subtilis

IolR

subtilis]

4079
IolS protein [Bacillus subtilis]
UniRef100_P46336

Bacillus subtilis

IolS

4080
UPI00003CB380 UniRef100 entry
UniRef100_UPI00003CB380

GlpQ

4081
Putative glycerol-3-phosphate
UniRef100_Q6GJY0

Staphylococcus

GlpT

transporter [Staphylococcus aureus]

aureus

4082
Chaperone protein htpG [Bacillus
UniRef100_P46208

Bacillus subtilis

HtpG

subtilis]

4083

YfmM

4084
Hypothetical protein yxeH [Bacillus
UniRef100_P54947

Bacillus subtilis

YxeH

subtilis]

4085
Glycerol dehydrogenase [Clostridium
UniRef100_Q97IL4

Clostridium

AraM

acetobutylicum]

acetobutylicum

4086
Phosphoserine phosphatase family
UniRef100_Q97IL5

Clostridium

YkrX

protein [Clostridium acetobutylicum]

acetobutylicum

4087
BH0833 protein [Bacillus halodurans]
UniRef100_Q9KEL9

Bacillus halodurans

4088

YxeB

4089

4090
BH3956 protein [Bacillus halodurans]
UniRef100_Q9K5Y0

Bacillus

YraH

halodurans

4091

4092
Ferrous iron transport protein B
UniRef100_Q632N2

Bacillus cereus ZK

[Bacillus cereus ZK]

4093
Lmo2104 protein [Listeria
UniRef100_Q929R6

Listeria monocytogenes

monocytogenes]

4094
YfiQ protein [Bacillus subtilis]
UniRef100_O31559

Bacillus subtilis

YfiQ

4095
ABC transporter permease protein
UniRef100_Q81EI1

Bacillus cereus

YxdM

[Bacillus cereus]

4096
ABC transporter ATP-binding protein
UniRef100_Q81D39

Bacillus cereus

YxdL

[Bacillus cereus]

4097
Sensor histidine kinase [Bacillus cereus
UniRef100_Q63AY4

Bacillus cereus

YxdK

ZK]

ZK

4098
UPI00003CBD9F UniRef100 entry
UniRef100_UPI00003CBD9F

YxdJ

4099
Hypothetical protein [Bacillus cereus]
UniRef100_Q72X82

Bacillus cereus

4100
YoaH [Bacillus subtilis]
UniRef100_O34576

Bacillus subtilis

YoaH

4101
Amino acid transporter [Bacillus
UniRef100_Q9K609

Bacillus

YflA

halodurans]

halodurans

4102
Alanine dehydrogenase [Bacillus
UniRef100_Q9KAF8

Bacillus

Ald

halodurans]

halodurans

4103
Hypothetical family 53 glycosyl
UniRef100_O07013

Bacillus subtilis

YvfO

hydrolase yvfO precursor [Bacillus

subtilis]

4104
Hypothetical protein yvfN [Bacillus
UniRef100_O07012

Bacillus subtilis

LacA

subtilis]

4105
Hypothetical protein yvfM [Bacillus
UniRef100_O07011

Bacillus subtilis

YvfM

subtilis]

4106
Maltose/maltodextrin transport system
UniRef100_Q9KBA8

Bacillus

YvfL

[Bacillus halodurans]

halodurans

4107
Hypothetical protein yvfK [Bacillus
UniRef100_O07009

Bacillus subtilis

YvfK

subtilis]

4108

LacR

4109
Galactokinase [Streptococcus gordonii]
UniRef100_Q840N8

Streptococcus

GalK

gordonii

4110
UDP-glucose 4-epimerase [Listeria
UniRef100_Q928B6

Listeria innocua

GalE

innocua]

4111
UPI00003CB9B6 UniRef100 entry
UniRef100_UPI00003CB9B6

GalT

4112
Transcriptional repressor of the xylose
UniRef100_Q9KDW7

Bacillus

XylR

operon [Bacillus halodurans]

halodurans

4113

GntR

4114

GntK

4115

GntP

4116

GntZ

4117
Alcohol dehydrogenase [Bacillus
UniRef100_Q818A4

Bacillus cereus

GbsB

cereus]

4118
Alkyl hydroperoxide reductase subunit
UniRef100_P80239

Bacillus subtilis

AhpC

C [Bacillus subtilis]

4119
NADH dehydrogenase [Bacillus subtilis]
UniRef100_P42974

Bacillus subtilis

AhpF

4120

4121

4122

4123

YvfR

4124
Hypothetical protein yvfS [Bacillus
UniRef100_O07017

Bacillus subtilis

YvfS

subtilis]

4125

YvfT

4126
Hypothetical protein yvfU [Bacillus
UniRef100_O07019

Bacillus subtilis

YvfU

subtilis]

4127

4128

4129

YtrE

4130

4131

4132

4133

4134
Hypothetical protein [Desulfotalea
UniRef100_Q6ARQ4

Desulfotalea psychrophila

psychrophila]

4135
Hypothetical protein [Desulfotalea
UniRef100_Q6ARQ5

Desulfotalea psychrophila

psychrophila]

4136

4137

4138
Putative zinc metallopeptidase
UniRef100_Q894N2

Clostridium tetani

[Clostridium tetani]

4139
Type I restriction-modification system
UniRef100_Q6MH63

Bdellovibrio bacteriovorus

restriction subunit [Bdellovibrio

bacteriovorus]

4140
Lin0523 protein [Listeria innocua]
UniRef100_Q92ED7

Listeria innocua

4141

4142
Hypothetical protein CAC1662
UniRef100_Q97IH9

Clostridium acetobutylicum

[Clostridium acetobutylicum]

4143
Type I restriction-modification system,
UniRef100_Q72BC7

Desulfovibrio vulgaris

M subunit [Desulfovibrio vulgaris]

4144

4145

4146

4147
UPI00002C7192 UniRef100 entry
UniRef100_UPI00002C7192

4148
Cassette chromosome recombinase B
UniRef100_Q8RPD2

Staphylococcus

SpoIVCA

[Staphylococcus aureus]

aureus

4149
Hypothetical UPF0247 protein yyda
UniRef100_Q45601

Bacillus subtilis

YydA

[Bacillus subtilis]

4150

4151

4152
YycN protein [Bacillus subtilis]
UniRef100_O32293

Bacillus subtilis

YycN

4153

4154
UPI00003CA59D UniRef100 entry
UniRef100_UPI00003CA59D

4155
Hypothetical serine protease yyxA
UniRef100_P39668

Bacillus subtilis

YyxA

[Bacillus subtilis]

4156
YycJ protein [Bacillus subtilis]
UniRef100_Q45611

Bacillus subtilis

YycJ

4157
YycI protein [Bacillus subtilis]
UniRef100_Q45612

Bacillus subtilis

YycI

4158
YycH protein [Bacillus subtilis]
UniRef100_Q45613

Bacillus subtilis

YycH

4159
Sensor protein yycG [Bacillus subtilis]
UniRef100_Q45614

Bacillus subtilis

YycG

4160
Transcriptional regulatory protein yycF
UniRef100_P37478

Bacillus subtilis

YycF

[Bacillus subtilis]

4161
Phosphohydrolase [Bacillus cereus]
UniRef100_Q81103

Bacillus cereus

4162
Adenylosuccinate synthetase [Bacillus
UniRef100_P29726

Bacillus subtilis

PurA

subtilis]

4163

4164
Replicative DNA helicase [Bacillus
UniRef100_P37469

Bacillus subtilis

DnaC

subtilis]

4165
Hypothetical protein yycD [Bacillus
UniRef100_P37480

Bacillus subtilis

subtilis]

4166
Hypothetical protein yyzB [Bacillus
UniRef100_O32296

Bacillus subtilis

subtilis]

4167
Hypothetical transport protein yycB
UniRef100_P37482

Bacillus subtilis

YycB

[Bacillus subtilis]

4168
N-acetylglucosamine-6-phosphate
UniRef100_Q9KFQ7

Bacillus

NagA

deacetylase [Bacillus halodurans]

halodurans

4169

NagB

4170
Transcriptional regulator [Bacillus
UniRef100_Q9KFQ9

Bacillus

YvoA

halodurans]

halodurans

4171
Hypothetical protein VP0543 [Vibrio
UniRef100_Q87S81

Vibrio

YbbI

parahaemolyticus]

parahaemolyticus

4172
PTS system, n-acetylglucosamine-
UniRef100_Q9KF24

Bacillus

NagP

specific enzyme II, ABC component

halodurans

[Bacillus halodurans]

4173
50S ribosomal protein L9 [Bacillus
UniRef100_P37437

Bacillus subtilis

RplI

subtilis]

4174
Hypothetical protein yybT [Bacillus
UniRef100_P37484

Bacillus subtilis

YybT

subtilis]

4175
Hypothetical protein yybS [Bacillus
UniRef100_P37485

Bacillus subtilis

YybS

subtilis]

4176
Spore coat protein F precursor [Bacillus
UniRef100_P23261

Bacillus subtilis

CotF

subtilis]

4177
Universal stress protein, Usp family
UniRef100_Q6HIV0

Bacillus

YxiE

[Bacillus thuringiensis]

thuringiensis

4178
Sulfate permease [Bacillus
UniRef100_Q6HIU9

Bacillus

YbaR

thuringiensis]

thuringiensis

4179
Hypothetical transport protein yybO
UniRef100_P37489

Bacillus subtilis

YybO

[Bacillus subtilis]

4180
30S ribosomal protein S18 [Bacillus
UniRef100_P21475

Bacillus subtilis

subtilis]

4181
Single-strand binding protein [Bacillus
UniRef100_P37455

Bacillus subtilis

Ssb

subtilis]

4182
30S ribosomal protein S6 [Bacillus
UniRef100_P21468

Bacillus subtilis

subtilis]

4183
GTP-dependent nucleic acid-binding
UniRef100_P37518

Bacillus subtilis

YyaF

protein engD [Bacillus subtilis]

4184
Lin2921 protein [Listeria innocua]
UniRef100_Q926W9

Listeria innocua

4185
Hypothetical protein yyaD [Bacillus
UniRef100_P37520

Bacillus subtilis

YyaD

subtilis]

4186
Hypothetical protein yyaC [Bacillus
UniRef100_P37521

Bacillus subtilis

YyaC

subtilis]

4187
Stage 0 sporulation protein J [Bacillus
UniRef100_P26497

Bacillus subtilis

Spo0J

subtilis]

4188

Soj

4189
Hypothetical protein [Burkholderia
UniRef100_Q63L10

Burkholderia

YdfG

pseudomallei K96243]

pseudomallei

K96243

4190
RNA polymerase sigma factor, ECF
UniRef100_Q63BC0

Bacillus cereus

SigM

subfamily [Bacillus cereus ZK]

ZK

4191
YyaA protein [Bacillus subtilis]
UniRef100_P37524

Bacillus subtilis

YyaA

4192
Methyltransferase gidB [Bacillus
UniRef100_P25813

Bacillus subtilis

GidB

subtilis]

4193
Glucose inhibited division protein A
UniRef100_P25812

Bacillus subtilis

GidA

[Bacillus subtilis]

4194
Probable tRNA modification GTPase
UniRef100_P25811

Bacillus subtilis

ThdF

trmE [Bacillus subtilis]

4195
Jag protein [Bacillus subtilis]
UniRef100_Q01620

Bacillus subtilis

Jag

4196

SpoIIIJ

4197
Ribonuclease P protein component
UniRef100_P25814

Bacillus subtilis

RnpA

[Bacillus subtilis]

TABLE 2

Features of the Bacillus licheniformis genome and comparison with genomes of other Bacillus species.

Feature

B. licheniformis

B. subtilis
^a

B. halodurans
^b

Oceanobacillus iheyensis
^c

B. anthracis
^d

B. cereus
^e

Chromosome Size (bp)
4,222,336
4,214,630
4,202,352
3,630,528
5,227,293
5,426,909

G + C content (mol %)
46.2
43.5
43.7
35.7
35.4
35.4

Protein coding sequences
4197
4106
4066
3496
5508
5366

Average length (bp)
873
896
879
883
800
835

Percent of coding region
87
87
85
85
84
84

Ribosomal RNA operons
7
10
8
7
11
13

Number of tRNAs
81
86
78
69
95
108

Phage-associated genes
71
268
42
27
62
124

Transposase genes of IS-elements
10
0
93
14
18
10

^aKunst, F., Ogasawara, N, Mozser, I., Albertini, A. M., Alloni, G., Azebedo, V., Bertero, M. G., Bessieres, P., Bolotin, A., and Borchert, S. et al. (1997) Nature 390, 249-256.

^bTakami, H., Nakasone, K., Takaki, Y., Maeno, G., Sasaki, R., Masui, N., Fuji, F., Hirama, C., Nakamura, Y., Ogasawara, N. et al. (2000) Nucleic Acids Res. 28, 4317-4331.

^cTakami, H., Takaki, Y., and Uchiyama, I. (2002) Nucleic Acids Res. 30, 3927-3935.

^dRead, T. D., Peterson, S. N., Tourasse, N., Baillie, L. W., Paulsen, I. T., Nelson, K. E., Tettelin, H., Fouts, D. E., Eisen, J. A., and Gill, S. R. et al. (2003) Nature 423, 81-86.

^eIvanova, N, Sorokin, A., Anderson, I, Galleron, N., Candelon, B., Kapatral, V., Bhattacharyya, A., Reznik, G., Mikhailova, N., and Lapidus, A. et al. (2003) Nature 423, 87-91.

TABLE 3

Extracellular proteins predicted in the Bacillus licheniformis genome

SEQ ID
Gene
Signal

B. subtilis

NO.
Name
Peptide Location
Putative Product
Putative Function
Gene

11
dacA
[1-32]
D-alanyl-D-alanine
“Molecular Function: serine
DacA

carboxypeptidase (penicillin-
carboxypeptidase activity

binding protein 5)
(GO:0004185), Biological

Process: proteolysis and

peptidolysis (GO:006508)”

45
yabE
[1-32]
conserved hypothetical containing domain DUF348 YabE
YabE

67
divlC
[1-66]
cell-division initiation protein
“required for both vegetative and
DivlC

sporulation septum formation,

Biological Process: cell cycle

(GO:0007049)”

157
BL01016
[1-28]
N-acetylmuramoyl-L-alanine
“cell wall hydrolase, Molecular
CwlD

amidase
Function: N-acetylmuramoyl-L-

alanine amidase activity

(GO:0008745), Biological

Process: peptidoglycan

catabolism (GO:0009253)”

159
gerD
[1-27]
GerD
“germination response to L-
GerD

alanine and to the combination

of glucose, fructose, L-

asparagine, and KCl (early

stage),”

161
ybaN
[1-42]
“Polysaccharide
“Biological Process:
YbaN

deacetylase, Carbohydrate
carbohydrate metabolism

Esterase Family 4”
(GO:0005975), Molecular

Function: hydrolase activity,

acting on carbon-nitrogen (but

not peptide) bonds

(GO:0016810)”

162
pbpX
[1-43]
penicillin-binding protein

PbpX

167
ybbC
[1-23]
conserved hypothetical protein YbbC
YbbC

168
ybbD
[1-27]
“Glycoside hydrolase, family 3”
“Molecular Function: hydrolase
YbbD

activity, hydrolyzing O-glycosyl

compounds (GO:0004553),

Biological Process: carbohydrate

metabolism (GO:0005975)”

169
ybbE
[1-22]
putative beta-lactamase YbbE

YbbE

227

[1-34]
putative ribose ABC transporter
“ribose transport,”
RbsB

(ribose-binding protein)

228
yomI
[1-32]
putative lytic transglycosylase YomI
YomI

247
penP
[1-35]
beta-lactamase precursor
“Molecular Function: aspartic-
PenP

type endopeptidase activity

(GO:0004190), Biological

Process: proteolysis and

peptidolysis (GO:0006508)”

249
tatAD
[1-35]
component of the twin-arginine
“Biological Process: protein transport

pre-protein translocation
(GO:0015031), Cellular Component: integral

pathway
to membrane (GO:0016021)”

267

[1-26]
“putative Proteinase inhibititor I4,
Molecular Function: serine-type

serpin”
endopeptidase inhibitor activity

(GO:0004867)

268
BL01663
[1-22]
Pectin lyase-like

YbdN

287
BL01793
[1-31]
putative lipoprotein

YcdA

303
yvbX
[1-33]
“conserved protein, Glycoside
“Molecular Function: catalytic
YvbX

Hydrolase Family 18, YvbX”
activity (GO:0003824), Biological

Process: carbohydrate

metabolism (GO:0005975)”

304

[1-27]
“Chitinase precursor, Glycoside
“Molecular Function: catalytic activity

Hydrolase Family 18”
(GO:0003824), Biological Process:

carbohydrate metabolism (GO:0005975)”

312
yvcE
[1-31]
“putative peptidoglycan hydrolase, DL-endopeptidase II family”
YvcE

348
yckB
[1-34]
putative extracellular solute-
“Molecular Function: transporter
YckB

binding protein YckB
activity (GO:0005215), Biological

Process: transport

(GO:0006810), Cellular

Component: periplasmic space

(sensu Gram-negative Bacteria)

(GO:0030288)”

353
nucA
[1-37]
nuclease NucA

NucA

359
BL01722
[1-34]
putative extracellular solute-
“Molecular Function: transporter
YvfK

binding protein
activity (GO:0005215), Biological

Process: transport

(GO:0006810)”

373

[1-25]
hypothetical protein

382
BL01829
[1-25]
“putative extracellular solute-
“Molecular Function: transporter
YckK

binding protein, family 3”
activity (GO:0005215), Biological

Process: transport

(GO:0006810), Cellular

Component: periplasmic space

(sensu Gram-negative Bacteria)

(GO:0030288)”

403
BL01746
[1-28]
ribose ABC transporter (ribose-
“ribose transport,”
RbsB

binding protein)

409
phy
[1-30]
phytase
“hydrolysis of phytate into
Phy

inorganic phosphate and myo-

inositol,”

426
yclQ
[1-25]
Periplasmic binding protein
“Molecular Function: iron ion
YclQ

transporter activity

(GO:0005381), Biological

Process: high affinity iron ion

transport (GO:0006827)”

438

[1-26]
hypothetical protein

472
BL02821
[1-34]
Cell envelope-related transcriptional attenuator
YvhJ

483

[1-42]
hypothetical protein

513
ydcC
[1-28]
conserved membrane protein YdcC
YdcC

552
yrhM
[1-68]
YrhM

YrhM

558
ywpE
[1-26]
conserved protein YwpE
“Biological Process: biosynthesis

(GO:0009058), Molecular Function:

transferase activity (GO:0016740)”

576

[1-34]
putative transporter
“Molecular Function: transporter activity

(GO:0005215), Biological Process: transport

(GO:0006810), Cellular Component:

membrane (GO:0016020)”

605
yjeAA
[1-37]
conserved hypothetical protein

YjeA

607
amyL
[1-30]
“alpha amylase, Glycoside
“Molecular Function: alpha-
TreA

Hydrolase Family 13”
amylase activity (GO:0004556),

Biological Process: carbohydrate

metabolism (GO:0005975)”

610
yvdG
[1-28]
putative maltose/maltodextrin
“Molecular Function: transporter
YvdG

transport system substrate-
activity (GO:0005215), Biological

binding protein
Process; transport

(GO:0006810)”

619

[1-37]
hypothetical protein

620

[1-28]
hypothetical protein

621
ydjM
[1-41]
YdjM

YdjM

622
ydjN
[1-21]
YdjN

YdjN

660
yerB
[1-28]
conserved protein YerB

YerB

665
yerH
[1-26]
YerH

YerH

683
BL05063
[1-35]
mannan endo-1,4-beta-
Degradation of mannan polysaccharides

mannosidase

733

[1-29]
putative carboxypeptidase
“Molecular Function: carboxypeptidase A

activity (GO:0004182), Biological Process:

proteolysis and peptidolysis (GO:0006508)”

773
yfkD
[1-26]
conserved protein YfkD

YfkD

776
BL03088
[1-24]
“Polysaccharide deacetylase,
“Biological Process:
YfjS

Carbohydrate Esterase Family
carbohydrate metabolism

4”
(GO:0005975), Molecular

Function: hydrolase activity,

acting on carbon-nitrogen (but

not peptide) bonds

(GO:0016810)”

784
yfjL
[1-33]
putative transporter YfjL
“Molecular Function: transporter
YfjL

activity (GO:0005215), Biological

Process: transport

(GO:0006810), Cellular

Component: membrane

(GO:0016020)”

787
BL03096
[1-26]
hypothetical protein

813
BL03024
[1-26]
“putative extracellular solute-
“Molecular Function: transporter
YtcQ

binding protein, family 1”
activity (GO:0005215), Biological

Process: transport

(GO:0006810)”

841
appAC
[1-30]
oligopeptide ABC transporter
“required for initiation of
OppA

(binding protein)
sporulation, competence

development, and oligopeptide

transport, Molecular Function:

transporter activity

(GO:0005215), Biological

Process: transport

(GO:0006810)”

866
ssuA
[1-24]
aliphatic sulfonate ABC transporter (binding lipoprotein)
SsuA

876

[1-23]
hypothetical protein

886

[1-23]
hypothetical protein

892

[1-28]
hypothetical protein

YhcC

900
BL03178
[1-27]
putative lipoprotein

YhcJ

902
BL03161
[1-31]
hypothetical protein

YhcM

904
BL03176
[1-29]
hypothetical protein

YhcN

905
BL03175
[1-30]
hypothetical protein

YhcP

934
lytE
[1-26]
cell wall hydrolase phosphalase-
“cell wall lytic activity, Biological
LytE

associated protein
Process: cell wall catabolism

(GO:0016998), Biological

Process: cell wall catabolism

(GO:0016998)”

937

[1-26]
putative glucose dehydrogenase

965
BL02841
[1-41]
“Polysaccharide deacetylase,
“Biological Process:
YheN

Carbohydrate Esterase Family
carbohydrate metabolism

4”
(GO:0005975), Molecular

Function: hydrolase activity,

acting on carbon-nitrogen (but

not peptide) bonds

(GO:0016810)”

996
prsA
[1-30]
molecular chaperone PrsA
“essential for the stability of
PrsA

secreted proteins at stages

following translocation across

the membrane, Molecular

Function: isomerase activity

(GO:0016853)”

1001
BL02907
[1-31]
hypothetical protein

YhaH

1032
yhfQ
[1-32]
putative transferase
“Biological Process; metabolism
YhfQ

(GO:0008152), Molecular

Function: transferase activity

(GO:0016740)”

1044
epr
[1-27]
extracellular serine protease
“Molecular Function: subtilase
Epr

activity (GO:0004289), Biological

Process: proteolysis and

peptidolysis

(GO:0006508), Molecular

Function: subtilase activity

(GO:0004289), Biological

Process: proteolysis and

peptidolysis (GO:0006508)”

1061

[1-24]
hypothetical protein

1063
msmE
[1-29]
multiple sugar-binding protein
“Molecular Function: transporter
MsmE

MsmE
activity (GO:0005215), Biological

Process: transport

(GO:0006810)”

1073
BL01323
[1-28]
conserved hypothetical protein

LytB

1077
yvgL
[1-29]
molybdate transport system
“Molecular Function: molybdate-
YvgL

substrate-binding protein
transporting ATPase activity

(GO:0015412), Biological

Process: molybdate ion transport

(GO:0015689)”

1113
BL01309
[1-24]
Short-chain
“Biological Process: metabolism
YcdF

dehydrogenase/reductase SDR
(GO:0008152), Molecular

Function: oxidoreductase activity

(GO:0016491)”

1150
appA
[1-32]
oligopeptide ABC transporter
“oligopeptide transport, Molecular
AppA

(oligopeptide-binding protein)
Function: transporter activity

(GO:0005215), Biological

Process: transport

(GO:0006810)”

1156
oppA
[1-29]
oligopeptide ABC transporter
“required for initiation of
OppA

(binding protein)
sporulation, competence

development, and oligopeptide

transport, Molecular Function:

transporter activity

(GO:0005215), Biological

Process: transport

(GO:0006810)”

1165
BL03332
[1-31]
conserved hypothetical protein

YflP

1216
abnAA
[1-26]
Glycoside Hydrolase Family 43
“degradation of plant cell wall
AbnA

polysaccharide, Molecular

Function: hydrolase activity,

hydrolyzing O-glycosyl

compounds (GO:0004553),

Biological Process: carbohydrate

metabolism (GO:0005975)”

1219

[1-30]
hypothetical protein

1226
BL01957
[1-23]
conserved hypothetical protein

YoeB

1227
BL01953
[1-26]
Peptidoglycan-binding protein
“Biological Process: cell wall
YocH

catabolism

(GO:0016998), Biological

Process: cell wall catabolism

(GO:0016998)”

1283
yesO
[1-28]
putative transport system
“Molecular Function: transporter
YesO

substrate-binding protein YesO
activity (GO:0005215), Biological

Process: transport

(GO:0006810)”

1291
yesW
[1-33]
putative polysaccharide lyase family 11 protein
YesW

1299
lplA
[1-32]
lipoprotein
“Molecular Function: transporter
LplA

activity (GO:0005215), Biological

Process: transport

(GO:0006810)”

1311
dppE
[1-27]
dipeptide ABC transporter
“Molecular Function: transporter
DppE

(dipeptide-binding protein)
activity (GO:0005215), Biological

Process: transport

(GO:0006810)”

1319
pelI
[1-25]
“pectate lyase family 1, PelI”

Pel

1332

[1-30]
hypothetical protein

1341

[1-27]
hypothetical protein

1345

[1-27]
hypothetical protein

YonS

1368

[1-28]
hypothetical protein

1415
pbpC
[1-30]
penicillin-binding protein 3
Molecular Function: penicillin
PbpC

binding (GO:0008658)

1448

[1-28]
hypothetical protein

1475
BL05139
[1-27]
glycerophosphoryl diester
“hydrolysis of deacylated
GlpQ

phosphodiesterase
phospholipids, Biological

Process: glycerol metabolism

(GO:0006071), Molecular

Function: glycerophosphodiester

phosphodiesterase activity

(GO:0008889)”

1479

[1-27]
hypothetical protein

1493

[1-27]
hypothetical protein

1496
BL03556
[1-25]
putative Cell wall hydrolase

YkvT

1498
ykvV
[1-28]
hypothetical protein
Contains thioredoxin domain 2
YkvV

1501

[1-25]
hypothetical protein

1512
ykwC
[1-24]
Hypothetical oxidoreductase

YkwC

1513
BL03646
[1-27]
Allergen V5/Tpx-1 related
Cellular Component:
YkwD

extracellular (GO:0005576)

1527

[1-28]
hypothetical protein

1553
BL01601
[1-32]
“putative secretion protein,
“Molecular Function: protein
YknX

protein transporter”
transporter activity

(GO:0008565), Biological

Process: protein secretion

(GO:0009306), Cellular

Component: membrane

(GO:0016020)”

1565

[1-40]
hypothetical protein

1576
ykyA
[1-29]
Hypothetical protein ykyA

YkyA

1601
BL02997
[1-27]
hypothetical protein

YlaJ

1617
ylbC
[1-34]
conserved hypothetical protein
Cellular Component:
YlbC

YlbC
extracellular (GO:0005576)

1625
ylbL
[1-29]
YlbL
Molecular Function: protein
YlbL

binding (GO:0005515)

1635
ftsL
[1-59]
cell-division protein
“septum formation (early stage),”
FtsL

1636
pbpB
[1-42]
penicillin-binding protein 2B
“formation of the cell-division
PbpB

septum (late stage),”

1644
divlB
[1-57]
cell-division initiation protein
“probably involved in stabilizing
DivlB

or promoting the assembly of the

division complex (septum

formation),”

1645
BL02246
[1-29]
conserved hypothetical protein

YlxW

1646
BL02248
[1-30]
hypothetical protein

YlxX

1650
bprA
[1-28]
bacillopeptidase F
“Molecular Function: subtilase
Bpr

activity (GO:0004289), Biological

Process: proteolysis and

peptidolysis (GO:0006508)”

1651
bprB
[1-31]
bacillopeptidase F
“Molecular Function: subtilase
Bpr

activity (GO:0004289), Biological

Process: proteolysis and

peptidolysis (GO:0006508)”

1752
fliL
[1-32]
flagellar protein
“required for flagellar
FliL

formation, Biological Process:

ciliary/flagellar motility

(GO:0001539), Biological

Process: chemotaxis

(GO:0006935), Cellular

Component: flagellar basal body

(sensu Bacteria) (GO:0009425)”

1781
celA
[1-34]
Glycoside Hydrolase Family 9
“Molecular Function: hydrolase activity,

hydrolyzing O-glycosyl compounds

(GO:0004553), Biological Process:

carbohydrate metabolism (GO:0005975)”

1783
celC
[1-34]
Glycoside Hydrolase Family 5
“Molecular Function: hydrolase activity,

hydrolyzing O-glycosyl compounds

(GO:0004553), Biological Process:

carbohydrate metabolism (GO:0005975)”

1784
celD
[1-32]
Glycoside Hydrolase family 5
“Molecular Function: hydrolase activity,

hydrolyzing O-glycosyl compounds

(GO:0004553), Biological Process:

carbohydrate metabolism (GO:0005975)”

1820
BL03655
[1-31]
conserved hypothetical protein

YmdA

1839

[1-29]
hypothetical protein

1904
yoaO
[1-38]
YoaO

YoaO

1914
yoaW
[1-26]
YoaW

YoaW

1926

[1-38]
phage-like protein

1929
nucB
[1-34]
nuclease

NucB

1931
BL05188
[1-29]
Peptidoglycan-binding protein
Biological Process: cell wall
YneA

catabolism (GO:0016998)

1946
yneN
[1-29]
putative thiol: disulfide
“Molecular Function: electron
YneN

interchange protein YneN
transporter activity

(GO:0005489), Biological

Process: electron transport

(GO:0006118)”

1983
bglC
[1-50]
“endo-1,4-beta-glucanase,
“Molecular Function: hydrolase
BglC

Glycoside hydrolase Family 5”
activity, hydrolyzing O-glycosyl

compounds (GO:0004553),

Biological Process: carbohydrate

metabolism (GO:0005975),”

1990
ywoF
[1-22]
“Polysaccharide Lyase Family 9, YwoF”
YwoF

1995
BL00297
[1-27]
conserved hypothetical protein

YddR

2011
yjmF
[1-31]
Short-chain
“Biological Process: metabolism
YjmF

dehydrogenase/reductase SDR
(GO:0008152), Molecular

Function: oxidoreductase activity

(GO:0016491)”

2016
dctB
[1-29]
possible C4-dicarboxylate
“, Biological Process: transport
DctB

binding protein
(GO:0006810), Cellular

Component: periplasmic space

(sensu Gram-negative Bacteria)

(GO:0030288)”

2028
dacC
[1-29]
penicillin-binding protein (D-
“Molecular Function: serine
DacC

alanyl-D-alanine
carboxypeptidase activity

carboxypeptidase)
(GO:0004185), Biological

Process: proteolysis and

peptidolysis (GO:0006508)”

2060

[1-37]
hypothetical protein

2072
BL00899
[1-28]
“Spore germination B3 GerAC like, C-terminal”
YndF

2105

[1-33]
conserved hypothetical protein

2108
BL01303
[1-26]
Peptidoglycan-binding protein
“Biological Process: cell wall
YocH

catabolism

(GO:0016998), Biological

Process: cell wall catabolism

(GO:0016998)”

2123

[1-36]
hypothetical protein

2132
BL01404
[1-24]
hypothetical protein

YoqH

2152
yvgO
[1-25]
conserved protein YvgO

YvgO

2160
lytF
[1-27]
gamma-D-glutamate-meso-
“cell wall lytic activity, Biological
LytF

diaminopimelate muropeptidase
Process: cell wall catabolism

(major autolysin) (CWBP49′)
(GO:0016998)”

2167
yoaJ
[1-26]
YoaJ

YoaJ

2177
ctpA
[1-37]
carboxy-terminal processing
“, Biological Process: proteolysis
CtpA

protease
and peptidolysis (GO:0006508),

Molecular Function: serine-type

peptidase activity

(GO:0008236)”

2180
yodJ
[1-31]
putative carboxypeptidase

YodJ

2207
ypmS
[1-34]
conserved protein YpmS

YpmS

2208
ypmR
[1-24]
conserved protein YpmR
Molecular Function: catalytic
YpmR

activity (GO:0003824)

2209
ypmQ
[1-25]
conserved hypothetical YpmQ
Biological Process: electron
YpmQ

transport (GO:0006118)

2218
ypjP
[1-30]
conserved hypothetical protein YpjP
YpjP

2266
ponA
[1-63]
“penicillin-binding
“involved in division septum
PonA

proteins, Glycosyl Transferase
formation,”

Family 51”

2271
aspB
[1-23]
aspartate aminotransferase
“Molecular Function:
AspB

transaminase activity

(GO:0008483), Biological

Process: biosynthesis

(GO:0009058)”

2272
ypmB
[1-25]
conserved protein YpmB

YpmB

2815
BL02789
[1-29]
hypothetical protein

YphF

2328
sleB
[1-34]
spore cortex-lytic enzyme

SleB

2330
ansZA
[1-18]
putative
“Biological Process: amino acid
YccC

Asparaginase/glutaminase
metabolism

(GO:0006520), Biological

Process: amino acid metabolism

(GO:0006520)”

2334
BL02228
[1-27]
putative hydrolase
Molecular Function: hydrolase
YpbG

activity (GO:0016787)

2344
yocH
[1-31]
putative peptidogycan hydrolase
“Biological Process: cell wall catabolism

YocH
(GO:0016998), Biological Process: cell wall

catabolism (GO:0016998)”

2347
BL00652
[1-32]
hypothetical protein

LytR

2348
yheN
[1-53]
“Carbohydrate Esterase Family
“Biological Process:
YheN

4, YheN”
carbohydrate metabolism

(GO:0005975), Molecular

Function: hydrolase activity,

acting on carbon-nitrogen (but

not peptide) bonds

(GO:0016810)”

2349

[1-29]
hypothetical protein

2360
dacB
[1-28]
D-alanyl-D-alanine
“required for spore cortex
DacB

carboxypeptidase (penicillin-
synthesis, Molecular Function:

binding protein 5*)
serine carboxypeptidase activity

(GO:0004185), Biological

Process: proteolysis and

peptidolysis (GO:0006508)”

2371
BL01892
[1-44]
conserved hypothetical protein

YpuD

2876
BL03279
[1-24]
conserved hypothetical protein

YpuA

2392
dacF
[1-33]
penicilin binding protein (putative
“required for spore cortex
DacF

D-alanyl-D-alanine
synthesis, Molecular Function:

carboxypeptidase)
serine carboxypeptidase activity

(GO:0004185), Biological

Process: proteolysis and

peptidolysis (GO:0006508)”

2400
BL01161
[1-28]
“Phosphotransferase system,
“Molecular Function: sugar porter activity

lactose/cellobiose-specific IIB
(GO:0005351), Biological Process:

subunit”
phosphoenolpyruvate-dependent sugar

Phosphotransferase system (GO:0009401)”

2418
lip
[1-31]
lipase
Molecular Function: catalytic
Lip

activity (GO:0003824)

2420
appAA
[1-32]
oligopeptide ABC transporter
“oligopeptide transport, Molecular
AppA

(oligopeptide-binding protein)
Function: transporter activity

(GO:0005215), Biological

Process: transport

(GO:0006810)”

2435

[1-24]
hypothetical protein

2436

[1-29]
hypothetical protein

2438
BL00808
[1-38]
hypothetical protein

YmaC

2449

[1-35]
hypothetical protein

2450

[1-31]
conserved hyopothetical protein
SsuA

2456
BL01380
[1-28]
“Phosphotransferase system,
“Molecular Function: sugar porter activity

lactose/cellobiose-specific IIB
(GO:0005351), Biological Process:

subunit”
phosphoenolpyruvate-dependent sugar

Phosphotransferase system (GO:0009401)”

2481
BL01514
[1-36]
Cell wall hydrolase/autolysin
“Molecular Function: N-
YqiI

acetylmuramoyl-L-alanine

amidase activity (GO:0008745),

Biological Process:

peptidoglycan catabolism

(GO:0009253)”

2482
yqiH
[1-33]
YqiH

YqiH

2484

[1-26]
hypothetical protein

2499
spoIIIAH
[1-32]
SpoIIIAH
“mutants block sporulation after
SpoIIIAH

engulfment,”

2505
spoIIIAB
[1-23]
SpoIIIAB
“mutants block sporulation after
SpoIIIAB

engulfment,”

2517
yqhL
[1-24]
conserved protein YqhL

YqhL

2518
opuAC
[1-31]
glycine betaine ABC transporter
“glycine betaine
OpuAC

(glycine betaine-binding protein)
transport, Molecular Function:

transporter activity

(GO:0005215), Molecular

Function: binding (GO:0005488),

Biological Process: transport

(GO:0006810)”

2528
tasA
[1-28]
translocation-dependent antimicrobial spore component
TasA

2530
yqxM
[1-44]
YqxM

YqxM

2531
yqzG
[1-24]
YqzG

YqzG

2533
comGG
[1-38]
probably part of the DNA
“required for exogenous DNA-
ComGG

transport machinery ComGG
binding,”

2546
yqgU
[1-28]
conserved protein YqgU

YqgU

2548
fhuD
[1-32]
ferrichrome ABC transporter
“Molecular Function: iron ion
FhuD

(ferrichrome-binding protein)
transporter activity

(GO:0005381), Biological

Process: high affinity iron ion

transport (GO:0006827)”

2560
yqzC
[1-33]
conserved protein YqzC

YqzC

2565
pstS
[1-33]
phosphate ABC transporter
“involved in high-affinity
PstS

(binding protein)
phosphate uptake, Molecular

Function; transporter activity

(GO:0005215), Biological

Process: transport

(GO:0006810)”

2566
pbpA
[1-44]
penicillin-binding protein 2A
“involved in the synthesis of
PbpA

peptidoglycan associated with

cell wall elongation in spore

outgrowth, Molecular Function:

penicillin binding (GO:0008658)”

2571
yqfZ
[1-40]
conserved protein YqfZ

2616
BL01411
[1-37]
conserved hypothetical protein

YqfA

2632
yqxA
[1-29]
YqxA

YqxA

2633
spoIIP
[1-55]
SpoIIP
“required for dissolution of the
SpoIIP

septal cell wall,”

2652
BL02075
[1-36]
putative lipoprotein
Molecular Function: catalytic
YqeF

activity (GO:0003824)

2697
appAB
[1-36]
oligopeptide ABC transporter
“oligopeptide transport, Molecular
AppA

(oligopeptide-binding protein)
Function: transporter activity

(GO:0005215), Biological

Process: transport

(GO:0006810)”

2706

[1-32]
putative phosphoesterase

2707
BL02428
[1-27]
carboxylesterase family
Molecular Function: catalytic
PnbA

activity (GO:0003824)

2710
BL03118
[1-28]
hypothetical protein

YndA

2712
sacC
[1-24]
Glycoside Hydrolase Family 32
“Molecular Function: hydrolase
SacC

activity, hydrolyzing O-glycosyl

compounds (GO:0004553),

Biological Process: carbohydrate

metabolism (GO:0005975)”

2729
BL00468
[1-33]
Periplasmic iron-binding protein
“Molecular Function: iron ion
YxeB

transporter activity

(GO:0005381), Biological

Process: high affinity iron ion

transport (GO:0006827)”

2745
yrrS
[1-50]
conserved protein YrrS

YrrS

2752
yrrL
[1-44]
conserved protein YrrL

YrrL

2771
yrvJ
[1-29]
N-acetylmuramoyl-L-alanine amidase YrvJ
YrvJ

2793
ymaC
[1-24]
phage-related protein YmaC

YmaC

2797
coxA
[1-27]
spore cortex protein

CoxA

2801
nadB
[1-37]
L-aspartate oxidase
“required for NAD biosynthesis,”
NadB

2818
mreC
[1-33]
cell-shape determining protein
“, Biological Process: regulation
MreC

of cell shape (GO:0008360)”

2866

[1-28]
hypothetical protein

2872
gerM
[1-34]
spore germination protein GerM
“germination (cortex hydrolysis)
GerM

and sporulation (stage II,

multiple polar septa),”

2898

[1-33]
hypothetical protein

2911
araN
[1-32]
sugar-binding protein
“L-arabinose transport, Molecular
AraN

Function: transporter activity

(GO:0005215), Biological

Process: transport

(GO:0006810)”

2916
abnAB
[1-28]
Glycoside Hydrolase Family 43
“degradation of plant cell wall
AbnA

polysaccharide, Molecular

Function: hydrolase activity,

hydrolyzing O-glycosyl

compounds (GO:0004553),

Biological Process: carbohydrate

metabolism (GO:0005975)”

2939
pelB
[1-31]
“pectate lyase, Polysaccharide Lyase Family 1”
PelB

3005
ytxE
[1-51]
YtxE
Cellular Component: outer
YtxE

membrane (sensu Gram-

negative Bacteria)

(GO:0009279)

3049
ytcQ
[1-30]
putative multiple sugar transport
“Molecular Function: transporter
YtcQ

system substrate-binding protein
activity (GO:0005215), Biological

YtcQ
Process: transport

(GO:0006810)”

3059
BL00013
[1-30]
putative lipoprotein

YusA

3081
ytlA
[1-33]
putative sulfonate transport system substrate-binding protein YtlA
YtlA

3088
ytkA
[1-29]
conserved protein YtkA

YtkA

3093
BL05310
[1-29]
Periplasmic solute binding
“Molecular Function: binding
YcdH

protein
(GO:0005488), Cellular

Component: periplasmic space

(sensu Gram-negative Bacteria)

(GO:0080288)”

3217

[1-22]
hypothetical protein
Molecular Function: catalytic activity

(GO:0003824)

3235
yuiC
[1-34]
conserved protein YuiC

YuiC

3264
yutC
[1-24]
conserved protein YutC

YutC

3266
BL02121
[1-22]
putative metallopeptidase
“Molecular Function:
YunA

metalloendopeptidase activity

(GO:0004222), Biological

Process: proteolysis and

peptidolysis (GO:0006508)”

3267

[1-32]
hypothetical protein

3269
yunB
[1-41]
conserved protein YunB

YunB

3284
yurYA
[1-40]
extracellular ribonuclease
Molecular Function: nuclease
YurI

activity (GO:0004518)

3298
yusA
[1-28]
putative ABC transport system substrate-binding protein YusA
YusA

3318
yfiY
[1-29]
ABC transport system substrate-
“Molecular Function: iron ion
YfiY

binding protein
transporter activity

(GO:0005381), Biological

Process: high affinity iron ion

transport (GO:0006827)”

3319
yusW
[1-28]
YusW

YusW

3333
gerAC
[1-25]
spore germination protein A3
“germination response to L-
GerAC

precursor
alanine and related amino acids

(earliest stage),”

3338
yvqG
[1-39]
conserved protein YvqG
“Biological Process: metabolism
YvqG

(GO:0008152), Molecular

Function: oxidoreductase activity

(GO:0016491)”

3349
yvrC
[1-37]
putative iron transport system
“Molecular Function: iron ion
YvrC

substrate-binding protein YvrC
transporter activity

(GO:0005381), Biological

Process: high affinity iron ion

transport (GO:0006827)”

3380
BL02684
[1-27]
putative Extracellular solute-
Molecular Function: transporter
YvfK

binding protein
activity (GO:0005215)

3384
BL02680
[1-31]
putative Glycoside Hydrolase Family 3
YbbD

3387
mntA
[1-28]
manganese transport system
“Molecular Function: binding
YcdH

substrate-binding protein
(GO:0005488), Cellular

Component: periplasmic space

(sensu Gram-negative Bacteria)

(GO:0030288)”

3429

[1-33]
hypothetical protein

3465

[1-32]
hypothetical protein

3492
opuCC
[1-32]
glycine betaine/carnitine/choline
“high affinity transport of glycine
OpuCC

ABC transporter
betaine, carnitine, and

(osmoprotectant-binding protein)
choline, Molecular Function:

transporter activity

(GO:0005215), Molecular

Function: binding (GO:0005488),

Biological Process: transport

(GO:0006810)”

3499
BL03470
[1-29]
“Periplasmic binding
“Molecular Function: iron ion
YfiY

protein, putative iron transporter”
transporter activity

(GO:0005381), Biological

Process: high affinity iron ion

transport (GO:00068271)”

3510

[1-26]
hypothetical protein

3547
sacB
[1-30]
“levansucrase, Glycoside
“Molecular Function: hydrolase
SacB

Hydrolase Family 68”
activity, hydrolyzing O-glycosyl

compounds (GO:0004553),

Biological Process: sugar

utilization (GO:0007587)”

3548
levB
[1-33]
“endolevanase, Glycoside Hydrolase Family 32”
YveB

3559

[1-29]
putative ribonuclease
“Molecular Function: RNA binding

(GO:0003723), Molecular Function:

endoribonuclease activity (GO:0004521)”

3578
yvpB
[1-39]
putative cysteine protease YvpB
“Molecular Function: cysteine-
YvpB

type endopeptidase activity

(GO:0004197), Biological

Process: proteolysis and

peptidolysis (GO:0006508)”

3579
yvpA
[1-29]
Polysaccharide Lyase Family 3
YvpA

3587
yvnB
[1-29]
YvnB

YvnB

3600
ctpB
[1-37]
“Peptidase S41A, C-terminal
“Biological Process: proteolysis
YvjB

protease”
and peptidolysis (GO:0006508),

Molecular Function: serine-type

peptidase activity (GO:0008236)

3602
BL03388
[1-29]
“Spectrin repeat, Rudiment single
“,”
YvcE

hybrid motif”

3643
lytC
[1-14]
N-acetylmuramoyl-L-alanine
“involved in cell separation, cell
LytC

amidase (major autolysin)
wall turnover, antibiotic-induced

(CWBP49)
lysis, motility and general cell

lysis induced by sodium

azide, Molecular Function: N-

acetylmuramoyl-L-alanine

amidase activity (GO:0008745),

Biological Process;

peptidoglycan catabolism

3644
lytB
[1-26]
modifier protein of major autolysin LytC (CWBP76)
LytB

3645
BL03297
[1-25]
membrane bound lipoprotein
involved in the secretion of
LytA

major autolysin

3646
lytR
[1-32]
membrane-bound protein
“attenuator role for lytABC and
LytR

lytR expression,”

3656
lytD
[1-29]
“N-acetylglucosaminidase (major
“involved in cell separation, cell
LytD

autolysin), Glycoside Hydrolase
wall turnover, antibiotic-induced

Family 73”
lysis and motility, Molecular

Function: amidase activity

(GO:0004040), Biological

Process: peptidoglycan

catabolism (GO:0009253)”

3670
pgdS
[1-34]
gamma-DL-glutamyl hydrolase
“gamma-DL-glutamyl hydrolase
YwtD

cleaving the gamma-glutamyl

bond only between D- and L-

glutamic acids of PGA, DL-

endopeptidase II family”

3671
ywtC
[1-25]
YwtC

3672
pgsAA
[1-47]
poly-gamma-glutamate synthesis protein
YwtB

3680
rbsB
[1-28]
ribose ABC transporter (ribose-
“ribose transport,”
RbsB

binding protein)

3738
feuA
[1-28]
iron-binding protein
“component of iron-uptake
FeuA

system, Molecular Function: iron

ion transporter activity

(GO:0005381), Biological

Process: high affinity iron ion

transport (GO:0006827)”

3751
ywmD
[1-25]
YwmD

YwmD

3752
ywmC
[1-24]
YwmC

YwmC

3753
spoIID
[1-33]
SpoIID
“required for complete
SpoIID

dissolution of the asymmetric,

septum,”

3755
ywmB
[1-30]
conserved protein YwmB

YwmB

3773
spoIIR
[1-29]
SpoIIR
required for processing of pro-
SpoIIR

sigma-E

3801
ywjE
[1-28]
putative Phospholipase
“Molecular Function: catalytic
YwjE

activity (GO:0003824), Biological

Process: metabolism

(GO:0008152)”

3808
BL03962
[1-41]
“Penicillin-binding protein,
“Molecular Function: penicillin
YwhE

Glycosyl Transferase Family 51”
binding (GO:0008658),

Biological Process: cell wall

biosynthesis (sensu Bacteria)

(GO:0009273)”

3812

[1-25]
hypothetical protein

3829
BL03904
[1-32]
hypothetical protein

YuaB

3849
vpr
[1-29]
extracellular serine protease
“Molecular Function: subtilase
Vpr

activity (GO:0004289), Biological

Process: proteolysis and

peptidolysis (GO:0006508)”

3906
ywaD
[1-31]
Putative aminopeptidase
“Biological Process: proteolysis
YwaD

and peptidolysis (GO:0006508),

Molecular Function: peptidase

activity (GO:0008233)”

3914
dltD
[1-27]
precusor DltD
D-alanine esterification of
DltD

lipoteichoic acid and wall

teichoic acid (D-alanine transfer

from undecaprenol-P to the

poly(glycerophosphate) chain of

LTA)

3920
licB
[1-24]
phosphotransferase system
“Molecular Function: sugar porter activity

(PTS) lichenan-specific enzyme
(GO:0005351), Biological Process:

IIB component
phosphoenolpyruvate-dependent sugar

phosphotransferase system (GO:0009401)”

3933
BL05372
[1-31]
hypothetical protein

YweA

3954
lanP
[1-25]
serine protease
“subtilase activity
IspA

(GO:0004289), proteolysis and

peptidolysis (GO:0006508),

lantibiotic leader peptide

processing”

3970
ansA
[1-29]
L-asparaginase
“Molecular Function:
AnsA

asparaginase activity

(GO:0004067), Biological

Process: amino acid metabolism

(GO:0006520)”

3977
BL02958
[1-25]
conserved hypothetical protein

YxeA

3978
yxkH
[1-28]
“Polysaccharide deacetylase,
“Biological Process:
YxkH

Carbohydrate Esterase Family
carbohydrate metabolism

4”
(GO:0005975), Molecular

Function: hydrolase activity,

acting on carbon-nitrogen (but

not peptide) bonds

(GO:0016810)”

3982
BL02963
[1-30]
Glycerophosphoryl diester
“Biological Process: glycerol
YhdW

phosphodiesterase
metabolism (GO:0006071),

Molecular Function:

glycerophosphodiester

phosphodiesterase activity

(GO:0008889)”

3983
BL05379
[1-28]
hypothetical protein

3996

[1-73]
hypothetical protein

4011
BL05383
[1-34]
hypothetical protein

YjeA

4016

[1-31]
hypothetical protein
Biological Process: electron transport

(GO:0006118)

4046
yxiA
[1-27]
“Glycoside hydrolase, family 43”
“Molecular Function: hydrolase
YxiA

activity, hydrolyzing O-glycosyl

compounds (GO:0004553),

Biological Process: carbohydrate

metabolism (GO:0005975)”

4058
ydaJ
[1-29]
putative Glycoside transferase

YdaJ

4080
glpQ
[1-29]
glycerophosphoryl diester
“hydrolysis of deacylated
GlpQ

phosphodiesterase
phospholipids, Biological

Process: glycerol metabolism

(GO:0006071), Molecular

Function: glycerophosphodiester

phosphodiesterase activity

(GO:0008889)”

4099
yxeA
[1-34]
conserved hypothetical protein

4103
yvfO
[1-28]
Glyccosyl Hydrolase Family 53
YvfO

4107
cycB
[1-82]
“putative extracellular solute-
“Molecular Function: transporter
YvfK

binding protein, family 1 CycB”
activity (GO:0005215), Biological

Process: transport

(GO:0006810)”

4121

[1-28]
hypothetical protein

4128

[1-34]
hypothetical protein

4130

[1-42]
hypothetical protein

4158
yycH
[1-36]
conserved hypothetical YycH

YycH

TABLE 4

Codon Usage Table for Chromosome

Amino

# Codon
acid
Fract
/1000
Number

GCA
A
0.230
18.394
22495

GCC
A
0.272
21.724
26567

GCG
A
0.301
24.082
29451

GCT
A
0.197
15.766
19281

TGC
C
0.662
5.113
6253

TGT
C
0.338
2.613
3195

GAC
D
0.432
22.129
27063

GAT
D
0.568
29.114
35605

GAA
E
0.704
51.721
63253

GAG
E
0.296
21.797
26657

TTC
F
0.354
16.034
19609

TTT
F
0.646
29.223
35738

GGA
G
0.316
22.644
27693

GGC
G
0.374
26.825
32806

GGG
G
0.171
12.287
15026

GGT
G
0.139
9.957
12177

CAC
H
0.386
8.412
10288

CAT
H
0.614
13.369
16350

ATA
I
0.101
7.217
8826

ATC
I
0.487
34.741
42487

ATT
I
0.412
29.376
35926

AAA
K
0.693
49.125
60078

AAG
K
0.307
21.739
26586

CTA
L
0.032
3.106
3798

CTC
L
0.152
14.848
18158

CTG
L
0.263
25.592
31298

CTT
L
0.229
22.330
27309

TTA
L
0.143
13.920
17023

TTG
L
0.181
17.639
21572

ATG
M
1.000
26.541
32458

AAC
N
0.505
19.159
23431

AAT
N
0.495
18.780
22967

CCA
P
0.112
4.189

CCC
P
0.120
4.477
5475

CCG
P
0.520
19.405
23731

CCT
P
0.248
9.236
11295

CAA
Q
0.465
16.656
20369

CAG
Q
0.535
19.179
23455

AGA
R
0.218
9.611
11754

AGG
R
0.146
6.448
7886

CGA
R
0.074
3.275
4005

CGC
R
0.253
11.173
13664

CGG
R
0.194
8.538
10441

CGT
R
0.114
5.044
6168

AGC
S
0.263
15.893
19436

AGT
S
0.063
3.820
4672

TCA
S
0.202
12.173
14887

TCC
S
0.154
9.287
11357

TCG
S
0.171
10.289
12583

TCT
S
0.147
8.865
10841

ACA
T
0.327
16.969
20752

ACC
T
0.188
9.765
11942

ACG
T
0.373
19.404
23730

ACT
T
0.112
5.833
7134

GTA
V
0.144
9.710
11875

GTC
V
0.361
24.342
29769

GTG
V
0.238
16.042
19619

GTT
V
0.257
17.354
21223

TGG
W
1.000
10.303
12600

TAC
Y
0.398
13.488
16471

TAT
Y
0.802
20.348
24885

TAA
*
0.551
1.982
2400

TAG
*
0.157
0.558
682

TGA
*
0.292
1.041
1273

TABLE 5

Signal Peptide Codon Usage Table

Amino

Codon
acid
Fract
/1000
Number

GCA
A
0.294
42.315
106

GCC
A
0.219
31.537
79

GCG
A
0.242
34.731
87

GCT
A
0.244
35.13
88

TGC
C
0.543
9.98
25

TGT
C
0.457
8.383
21

GAC
D
0.231
2.395
6

GAT
D
0.769
7.984
20

GAA
E
0.784
15.968
40

GAG
E
0.216
4.391
11

TTC
F
0.335
21.956
55

TTT
F
0.665
43.513
109

GGA
G
0.341
22.355
56

GGC
G
0.262
17.166
43

GGG
G
0.183
11.976
30

GGT
G
0.213
13.972
35

CAC
H
0.316
2.395
6

CAT
H
0.684
5.19
13

ATA
I
0.142
9.98
25

ATC
I
0.466
32.735
82

ATT
I
0.392
27.545
69

AAA
K
0.767
61.876
155

AAG
K
0.233
18.762
47

CTA
L
0.05
7.186
18

CTC
L
0.098
13.972
35

CTG
L
0.235
33.533
84

CTT
L
0.16
22.754
57

TTA
L
0.221
31.537
79

TTG
L
0.235
33.533
84

ATG
M
1
51.497
129

AAC
N
0.482
10.778
27

AAT
N
0.518
11.577
29

CCA
P
0.164
4.79
12

CCC
P
0.192
5.589
14

CCG
P
0.452
13.174
33

CCT
P
0.192
5.589
14

CAA
Q
0.475
11.577
29

CAG
Q
0.525
12.774
32

AGA
R
0.333
9.98
25

AGG
R
0.08
2.395
6

CGA
R
0.107
3.194
8

CGC
R
0.18
4.79
12

CGG
R
0.173
5.19
13

CGT
R
0.147
4.391
11

AGC
S
0.171
14.77
37

AGT
S
0.093
7.984
20

TCA
S
0.241
20.758
52

TCC
S
0.148
12.774
32

TCG
S
0.134
11.577
29

TCT
S
0.213
18.363
46

ACA
T
0.262
11.178
28

ACC
T
0.234
9.98
25

ACG
T
0.327
13.972
35

ACT
T
0.178
7.585
19

GTA
V
0.216
14.371
36

GTC
V
0.186
12.375
31

GTG
V
0.335
22.355
56

GTT
V
0.263
17.565
44

TGG
W
1
7.585
19

TAC
Y
0.405
5.988
15

TAT
Y
0.595
8.782
22

TAA
*
0
0
0

TAG
*
0
0
0

TGA
*
0
0
0

Example 6
Identification of Genes Induced by Glucose Limitation Using DNA Microarrays

Bacillus licheniformis ATCC 14580 was grown in duplicate shake flasks containing 100 ml of Spizizen I medium (Anagnostopoulos and Spizizen, 1961, Journal of Bacteriology 81: 741-748). The inocula for the shake flasks were obtained from 10 ml of mid-log cells (approximately 80-90 Klett units) and a 0 hour total RNA sample was extracted from 30 ml of the mid-log cells, as described below. Total cellular RNA was also purified from 10 ml of the Spizizen I shake flasks that were sampled at 2, 4, 6 and 8 hours post inoculum. All of the cell samples were mixed with two volumes of RNAProtect RNA stabilizing reagent (QIAGEN, Inc., Valencia, Calif.) for 5 minutes. The cells were subsequently pelleted at 2800×g, 4° C. for 10 minutes, the supernatant was discarded, the cell pellets were frozen at −80° C., and cellular RNA was purified using a FastRNA Pro Blue kit (QBiogene, Carlsbad, Calif.) using the protocol supplied by the manufacturer. The frozen cell pellets from each sample were resuspended in 2 ml RNAPro solution provided in the FastRNA Pro Blue kit and RNA was obtained from two lysis matrix B vials.

Sixteen replicate cDNA targets for the 0 time point and two technical replicate cDNA targets for each of the rest of the time points were prepared and hybridized to Bacillus licheniformis DNA microarrays prepared as described by Berka et al., 2002, Molecular Microbiology 43: 1331-1345. Dye swap hybridizations using the zero time point as reference were prepared as described in Hu et al. [In G. Parmigiani, E. S. Garrett, R. A Irizarry, and S. L. Zeger (eds.) The Analysis of Gene Expression Data, pp. 318-319, Springer-Verlag, New York (2003)].

The arrays were scanned with an Axon 4000B scanner and formatted for analysis with GenePix Pro version 5.0 (Axon Instruments, Inc. Redwood City, Calif.). The fluorescence intensity data from GenePix was imported directly into S-Plus ArrayAnalyzer version 2 software (Insightful Corporation, Seattle, Wash.) for statistical analysis. The raw intensity data were normalized using the lowess (locally weighted regression) function provided in S-Plus ArrayAnalyzer software, and the genes that were differentially expressed at each time point relative to the time zero reference were identified by employing a multiple comparison t-test with Bonferroni correction as outlined in the user's guide to S-Plus ArrayAnalyzer software (Insightful Corp., Seattle, Wash.). The family-wise error rate (FWER) was set at 0.1. The list of genes at each time point that passed these statistical criteria were used to query a pathway-genome database established using Pathway Tools Suite software (Karp at al., 2002. Bioinformatics 18 suppl. 1: S225-S232), and the gene expression profiles were painted onto a schematic of metabolic pathways. In doing so, those pathways that were significantly altered at each time point were identified.

As a result of this analysis the following observations were made: (1) Early in the cultures, genes encoding enzymes of the glycolytic pathway were slightly induced reflecting the change from partially depleted glucose levels in the inoculum culture to ample glucose in the shake flask medium; (2) As glucose was progressively depleted in the shake flask culture (times 6 and 8 hours), genes encoding glycolytic pathway enzymes were down-regulated; (3) genes that encode enzymes for phenylalanine, tryptophan, histidine, and arginine biosynthetic pathways were up-regulated during stationary phase suggesting that cells had exhausted critical amino acids, and they were turning on the genes for pathways to synthesize more of these essential nutrients; (4) as cells entered stationary phase, cell division, DNA replication, and ribosome production all decreased to a low level. This was reflected in the shut-down of purine and pyrimidine biosynthetic pathways and down-regulation of genes encoding many ribosomal proteins.

The invention described and claimed herein is not to be limited in scope by the specific aspects herein disclosed, since these aspects are intended as illustrations of several aspects of the invention. Any equivalent aspects are intended to be within the scope of this invention. Indeed, various modifications of the invention in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims. In the case of conflict, the present disclosure including definitions will control.

Various references are cited herein, the disclosures of which are incorporated by reference in their entireties.

Number	Name	Date	Kind
5589381	Neyra et al.	Dec 1996	A
5665354	Neyra et al.	Sep 1997	A
6060241	Corthesy-Theulaz	May 2000	A
6506581	Fleischmann et al.	Jan 2003	B1
6528289	Fleischmann et al.	Mar 2003	B1
6593114	Kunsch et al.	Jul 2003	B1
6846651	Fleischmann et al.	Jan 2005	B2
7018794	Berka et al.	Mar 2006	B2
7691574	Berka et al.	Apr 2010	B2
20020146721	Berka et al.	Oct 2002	A1

Number	Date	Country
WO 0229113	Apr 2002	WO
WO 0229113	Apr 2002	WO
WO 03000941	Jan 2003	WO
WO 03054163	Jul 2003	WO
WO 03087149	Oct 2003	WO

Number	Date	Country
60535988	Jan 2004	US
60561059	Apr 2004	US
60572403	May 2004	US

	Number	Date	Country
Parent	12322974	Feb 2009	US
Child	12972306		US
Parent	10983128	Nov 2004	US
Child	12322974		US

Bacillus licheniformis chromosome

Information

Patent Number

Date Filed

Date Issued

Inventors

Original Assignees

Examiners

Agents

CPC

US Classifications

Field of Search

US

International Classifications

Abstract

Description

Claims

CROSS-REFERENCE TO RELATED APPLICATIONS

US Referenced Citations (10)

Foreign Referenced Citations (5)

Related Publications (1)

Provisional Applications (3)

Divisions (2)