Patent Application
20150250906
Diagnosis of infectious diseases by standard diagnostic tools, namely microscopy and microbiologic culture are often limited due to difficult sampling of obscure or deep sites of infection such as bone, cardiac, central nervous system, pulmonary or intra-abdominal locations. Rapid diagnosis of infectious disease is critical for administering appropriate antibiotic therapy, especially in modern patient populations who may be immunocompromised by HIV infection, solid organ transplant, autoimmune, or cancer chemotherapies. Traditional diagnostic methods are dependent upon sampling suspected sites of infection, then performing culture or molecular techniques. This approach is both labor intensive and time consuming, and is subject to the uncertainties of incorrect sampling and contamination. It may also be dangerous (e.g. brain biopsies or biopsies in patients with bleeding risk) or sometimes impossible (e.g. patients who are at high risk for complications secondary to anesthesia).
Complimentary techniques, such as cross sectional imaging by computed tomography or magnetic resonance imaging may provide a site of interest for invasive tissue sampling, but reveal structural abnormalities which are often a late occurrence in the disease process. Furthermore, normal structure may be perturbed following surgical procedures, and indolent infections may not produce significant morphologic changes. Standard imaging is therefore a lagging indicator, and only by repeated imaging is the success of antimicrobial therapy inferred. Nuclear medicine imaging techniques, such as 18F-fluorodeoxyglucose positron emission tomography have the potential to noninvasively determine the location and severity of infection as well as the response to therapy, but unfortunately are dependent upon host white blood cell function, and cannot differentiate between cancer, sterile inflammation or infection. As a result, there remains a need for prokaryote specific radiopharmaceutical agents that can rapidly and accurately diagnose and monitor infection.
The compositions and methods of the present invention exploit unique biochemical pathways present within infectious organisms, such as prokaryotes, to develop a series of small molecule metabolic tracers. Potential imaging agents were selected by three major criteria: 1) Presence of prokaryote specific metabolic pathway 2) Absence of known host metabolism and 3) Evidence for accumulation of tracer or essentiality of the pathway for bacterial survival.
In accordance with an embodiment, the present invention provides a method for detection of the presence of infectious organisms in a mammalian host comprising: a) administering to the host a therapeutically effective amount of a compound comprising a labeled substrate, wherein the substrate is a compound that is solely utilized by the metabolism of the infectious organism selected from the group consisting of prokaryotes, fungi, protozoa, and virally infected host cells; and wherein the label of the labeled substrate is a detectable moiety selected from the group consisting of radionuclides, near-infrared dyes, fluorescent dyes, PET, SPECT and MRI imaging agents; b) allowing a sufficient period of time for the infectious organism to take up the labeled substrate; and c) determining whether the infectious organisms are present in the host by detecting the labeled substrate in the body of the mammalian host.
In accordance with another embodiment, the present invention provides a method for detection of the location of infectious organisms in the body of a mammalian host comprising: a) administering to the host a therapeutically effective amount of a compound comprising a labeled substrate, wherein the substrate is a compound that is solely utilized by the metabolism of the infectious organism selected from the group consisting of prokaryotes, fungi, protozoa, and virally infected host cells; and wherein the label of the labeled substrate is a detectable moiety selected from the group consisting of radionuclides, near-infrared dyes, and fluorescent dyes; b) allowing a sufficient period of time for the infectious organism to take up the labeled substrate; and c) determining the location of the infectious organisms present in the host by detecting the labeled substrate in the body of the mammalian host.
In accordance with an embodiment, the present invention provides a method for detection of the presence of gram negative organisms in a mammalian host comprising: a) administering to the host a therapeutically effective amount of a labeled substrate comprising labeled sorbitol and/or derivatives thereof; b) allowing a sufficient period of time for the gram negative organisms to take up the labeled substrate; and c) determining whether the gram negative organisms are present in the host by detecting the labeled substrate in the body of the mammalian host.
In accordance with an embodiment, the present invention provides a method for detection of the presence of gram negative organisms in a mammalian host comprising: a) administering to the host a therapeutically effective amount of a labeled substrate comprising 18F-fluorodeoxysorbitol (FDS) and/or derivatives thereof; b) allowing a sufficient period of time for the gram negative organisms to take up the labeled substrate; and c) determining whether the gram negative organisms are present in the host by detecting the labeled substrate in the body of the mammalian host.
In accordance with another embodiment, the present invention provides a method for the determination of the efficacy of an antibiotic therapy in a mammalian host with a gram negative infection comprising: a) administering to the host a therapeutically effective amount of a labeled substrate comprising 18F-fluorodeoxysorbitol ([18F]FDS) and/or derivatives thereof; b) allowing a sufficient period of time for the gram negative organism to take up the labeled substrate; c) determining the amount gram negative organisms are present in the host by detecting the labeled substrate in the body of the mammalian host; d) subjecting the mammalian host to antibiotic treatment for a selected period of time; e) repeating steps a)-c) one or more times; f) assessing whether the gram negative infection has been reduced after d) by comparing the amount of labeled substrate present before d) to the amount of labeled substrate present after d), wherein when the amount of labeled substrate present after d) is less, than a determination is made that the antibiotic treatment of d) is effective.
In accordance with a further embodiment, the present invention provides a method for the determination of the efficacy of an antibiotic therapy in a mammalian host with an infection comprising: a) administering to the host a therapeutically effective amount of a compound comprising a labeled substrate, wherein the substrate is a compound that is solely utilized by the metabolism of the infectious organisms selected from the group consisting of prokaryotes, fungi, protozoa, and virally infected host cells; and wherein the label of the labeled substrate is a detectable moiety selected from the group consisting of radionuclides, near-infrared dyes, and fluorescent dyes; b) allowing a sufficient period of time for the infectious organism to take up the labeled substrate; c) determining the amount infectious organisms are present in the host by detecting the labeled substrate in the body of the mammalian host; d) subjecting the mammalian host to antibiotic treatment for a selected period of time; e) repeating steps a)-c) one or more times; f) assessing whether the infection has been reduced after d) by comparing the amount of labeled substrate present before d) to the amount of labeled substrate present after d), wherein when the amount of labeled substrate present after d) is less, than a determination is made that the antibiotic treatment of d) is effective.
In accordance with yet another embodiment, the present invention provides a method for identification of infectious prokaryotic organisms in a mammalian host comprising: a) administering to the host a therapeutically effective amount of two or more different compounds comprising a labeled substrate, wherein the substrate is a compound that is solely utilized by the metabolism of the infectious organisms selected from the group consisting of prokaryotes, fungi, protozoa, and virally infected host cells; and wherein the label of the labeled substrate is a detectable moiety selected from the group consisting of radionuclides, near-infrared dyes, and fluorescent dyes; b) allowing a sufficient period of time for the infectious organism to take up the labeled substrate; and c) determining the identity of infectious prokaryotic organisms present in the host by detecting and quantifying the amount of each of the labeled substrates in the body of the mammalian host and comparing the quantities of each labeled substrate to a control for the specific species of prokaryotic organisms.
In accordance with an embodiment, the present invention provides a method for the determination of the appropriate an antibiotic therapy in a mammalian host with an infection comprising: a) administering to the host a therapeutically effective amount of two or more different compounds comprising a labeled substrate, wherein the substrate is a compound that is solely utilized by the metabolism of the infectious organisms selected from the group consisting of prokaryotes, fungi, protozoa, and virally infected host cells; and wherein the label of the labeled substrate is a detectable moiety selected from the group consisting of radionuclides, near-infrared dyes, and fluorescent dyes; b) allowing a sufficient period of time for the infectious organism to take up the labeled substrate; c) determining the identity of infectious prokaryotic organisms present in the host by detecting and quantifying the amount of each of the labeled substrates in the body of the mammalian host and comparing the quantities of each labeled substrate to a control for the specific species of prokaryotic organisms; and d) selecting the appropriate antibiotic therapy after identification of the specific species of prokaryotic organisms present in the mammalian host.
The methods of the present invention introduce an approach that has yielded a number of candidate molecular probes of infectious organisms, such as bacterial organisms, that are suitable for use as labeled substrates, such as radiolabeled, or dye labeled tracers. Previously proposed tracers, such as FIAU, require the presence of bacterial thymidine kinase, which is not present in some species of bacteria, such as Mycobacterium tuberculosis. The enzyme for folic acid synthesis from PABA, dihydropteroate synthase is widely expressed among species of bacteria, and moreover is synthesized in many other pathogens, such as pneumocystis jiroveci, toxoplasma gondii and plasmodium species.
In accordance with one or more embodiments of the present invention, the methods presented herein exploit unique biochemical pathways present within infectious organisms to develop a series of small molecule metabolic tracers that have the potential to diagnose and monitor the presence of infection (first order; differentiate infection from inflammation or other processes such as malignancies), organism identification (second order) and antimicrobial susceptibility (third order; information on antibiotic efficacy).
Therefore, in accordance with one or more embodiments of the present invention, labeled substrates including, for example, p-aminobenzoic acid (PABA), D-xylose (XYL), 2,6-diaminopimellic acid (DAP), methyl-alpha-D-glucopyranoside (MGP), shikimate (SHIK), cellobiose (CB) mannitol (MAN), and sorbitol (SOR) are useful for detection of a constellation of infectious diseases. Other embodiments include, but are not limited to sugars such as mannitol or sorbitol or derivatives thereof, and embodiments other sugars such or labeled substrates outlined above.
As used herein, the term “substrate” means any refers to a compound or protein or peptide or other biologically active molecule which is metabolized or otherwise taken up by a specific target infectious organism, such as, for example, a bacteria, but is not utilized in any significant amount, in the cellular metabolism of a mammalian host.
Examples of such compounds include compounds metabolized by prokaryote-specific pathways, 2) evidence for prokaryote accumulation or antimicrobial activity, and 3) absence of known eukaryotic accumulation or metabolism of these compounds.
As used herein, the term “labeled” means a compound or protein or peptide or other biologically active molecule which has a detectable moiety linked to it either covalently or via a linking molecule.
By “detectable label(s) or moieties” is meant a composition that when linked to a molecule of interest renders the latter detectable, via spectroscopic, photochemical, biochemical, immunochemical, chemical means or other imaging means. For example, useful labels include radioactive isotopes, magnetic beads, metallic beads, colloidal particles, fluorescent dyes, electron-dense reagents, enzymes (for example, as commonly used in an ELISA), biotin, digoxigenin, or haptens. Specific radioactive labels include most common commercially available isotopes including, for example, 3H, 11C, 13C, 15N, 18F, 19F, 123I, 124I, 125I, 131I, 86Y, 89Zr, 111In, 94mTc, 99mTc, 64Cu and 68Ga. Suitable dyes include any commercially available dyes such as, for example, 5(6)-carboxyfluorescein, IRDye 680RD maleimide or IRDye 800CW, ruthenium polypyridyl dyes, and the like. Also included in the labeled substrates of the present invention are substrates labeled with PET, SPECT or MRI detectable imaging agents or moieties.
In accordance with an embodiment, the labeled substrates of the present invention include 18F labeled substrates. Examples of such substrates include, but are not limited to, 2-fluoro-PABA, 3-fluoro-PABA, and 2-fluoro-mannitol. Other fluorinated substrates identified here are also within the scope of the present invention.
As used herein, the term “contacting” refers to any suitable method of bringing a labeled substrate suitable for imaging into contact with an infectious organism, e.g., bacteria, fungi, protozoa and virally infected cells of the host mammal. The term “contacting” can also refer to bringing a labeled substrate suitable for imaging into contact with an infectious organism, e.g., bacteria, fungi, protozoa and virally infected cells in vitro.
One of ordinary skill in the art would understand that use of the term “solely utilized by the metabolism of the infection organism” is meant to convey the concept that the labeled substrate is a significant substrate in one or more metabolic pathways of the infectious organism, while it has little or no significant use as a substrate in the metabolic activity of normal cells in the mammalian host.
In accordance with an embodiment, the present invention provides a method for detection of the presence of infectious organisms in a mammalian host comprising: a) administering to the host a therapeutically effective amount of a compound comprising a labeled substrate, wherein the substrate is a compound that is solely utilized by the metabolism of the infectious organisms selected from the group consisting of prokaryotes, fungi, protozoa, and virally infected host cells; and wherein the label of the labeled substrate is a detectable moiety selected from the group consisting of radionuclides, near-infrared dyes, and fluorescent dyes; b) allowing a sufficient period of time for the infectious organism to take up the labeled substrate; and c) determining whether the infectious organisms are present in the host by detecting the labeled substrate in the body of the mammalian host.
As used herein, the term “infectious organisms” includes microbiological organisms that can infect the host mammal Prokaryotic organisms, such as bacteria, both gram positive and gram negative bacteria are included. One of ordinary skill would understand that in the context of the methods of the present invention, the definition also is inclusive of other microbiological organisms which can infect a mammalian host and which have at least one or more metabolic processes or enzymes that are not found in any significant amounts in the mammalian host, which can be the target of the labeled substrates of the present invention. As such, organisms such as fungi, protozoa, parasites and also mammalian cells which have been infected by certain viruses.
In accordance with an embodiment, the detectable moiety is a fluorescent dye. The dyes may be emitters in the visible or near-infrared (NIR) spectrum. Known dyes useful in the present invention include carbocyanine, indocarbocyanine, oxacarbocyanine, thüicarbocyanine and merocyanine, polymethine, coumarine, rhodamine, xanthene, fluorescein, boron˜dipyrromethane (BODIPY), Cy5, Cy5.5, Cy7, VivoTag-680, VivoTag-S680, VivoTag-S750, AlexaFluor660, AlexaFluor680, AlexaFluor700, AlexaFluor750, AlexaFluor790, Dy677, Dy676, Dy682, Dy752, Dy780, DyLight547, Dylight647, HiLyte Fluor 647, HiLyte Fluor 680, HiLyte Fluor 750, IRDye 800CW, IRDye 800RS, IRDye 700DX, ADS780WS, ADS830WS, and ADS832WS.
Organic dyes which are active in the NIR region are known in biomedical applications. However, there are only a few NIR dyes that are readily available due to the limitations of conventional dyes, such as poor hydrophilicity and photostability, low quantum yield, insufficient stability and low detection sensitivity in biological system, etc. Significant progress has been made on the recent development of NIR dyes (including cyanine dyes, squaraine, phthalocyanines, porphyrin derivatives and BODIPY (borondipyrromethane) analogues) with much improved chemical and photostability, high fluorescence intensity and long fluorescent life. Examples of NIR dyes include cyanine dyes (also called as polymethine cyanine dyes) are small organic molecules with two aromatic nitrogen-containing heterocycles linked by a polymethine bridge and include Cy5, Cy5.5, Cy7 and their derivatives. Squaraines (often called Squarylium dyes) consist of an oxocyclobutenolate core with aromatic or heterocyclic components at both ends of the molecules, an example is KSQ-4-H. Phthalocyanines, are two-dimensional 18π-electron aromatic porphyrin derivatives, consisting of four bridged pyrrole subunits linked together through nitrogen atoms. BODIPY (borondipyrromethane) dyes have a general structure of 4,4′-difluoro-4-bora-3a, 4a-diaza-s-indacene) and sharp fluorescence with high quantum yield and excellent thermal and photochemical stability.
Other imaging agents which can be used in the labeled substrates of the present invention include PET and SPECT imaging agents. The most widely used agents include branched chelating agents such as di-ethylene tri-amine penta-acetic acid (DTPA), 1,4,7,10-tetra-azacyclododecane-1,4,7,10-tetraacetic acid (DOTA) and their analogs. Chelating agents, such as di-amine dithiols, activated mercaptoacetyl-glycyl-glycyl-glycine (MAG3), and hydrazidonicotinamide (HYNIC), are able to chelate metals like 99mTc and 186Re. Instead of using chelating agents, a prosthetic group such as N-succinimidyl-4-18F-fluorobenzoate (18F-SFB) is necessary for labeling peptides with 18F.
In accordance with another embodiment, the present invention provides a labeled substrate, wherein the label is covalently linked to the substrate and the label comprises a compound or enzyme that produces light or a photon, which is detectable when in the presence of a substrate in the target microorganism. An example of such a substrate is luciferase, where in the presence of ATP within the organism, luciferase will react with ATP and produce a photon. One of ordinary skill in the art will understand that there are other enzyme and reporter complexes which can be used in the art.
In accordance with an embodiment, the detectable moiety may be attached to the substrate by a linker molecule. For instance linking groups having alkyl, aryl, combination of alkyl and aryl, or alkyl and aryl groups having heteroatoms may be present. For example, the linker can be a C1-C20 alkyl, C2-C20 alkenyl, C2-C20 alkynyl, C1-C20 hydroxyalkyl, C1-C20 alkoxy, C1-C20 alkoxy C1-C20 alkyl, C1-C20 alkylamino, di-C1-C20 alkylamino, C1-C20 dialkylamino C1-C20 alkyl, C1-C20 thioalkyl, C2-C20 thioalkenyl, C2-C20 thioalkynyl, C6-C22 aryloxy, C6-C22 arylamino C2-C20 acyloxy, C2-C20 thioacyl, C1-C20 amido, and C1-C20 sulphonamido.
As used herein, the term “therapeutically effective amount” means that the one or more labeled substrates are administered to a subject in vivo in an amount that is sufficient to effectively target the infectious organisms of interest and which can be detected in the subject over a reasonable time frame. In other embodiments, the term “therapeutically effective amount” means that the one or more labeled substrates are placed in contact with the infectious organisms of interest in vitro and which can be detected in the organisms over a reasonable time frame.
In accordance with an embodiment, the present invention provides a pharmaceutical composition comprising one or more labeled substrates described herein and a pharmaceutically acceptable carrier.
In another embodiment, the present invention provides a pharmaceutical composition comprising one or more labeled substrates described herein, a pharmaceutically active compound, and a pharmaceutically acceptable carrier.
In accordance with an embodiment, the present invention provides a use of the labeled substrates described herein, in an effective amount, to prepare a medicament, preferably for use as a medicament for treating a disease in a subject.
In accordance with an embodiment, the present invention provides a pharmaceutical composition comprising a labeled substrate, wherein the substrate is a compound that is solely utilized by the metabolism of the infectious organisms selected from the group consisting of prokaryotes, fungi, protozoa, and virally infected host cells; and wherein the label of the labeled substrate is a detectable moiety selected from the group consisting of radionuclides, near-infrared dyes, and fluorescent dyes; wherein the composition includes a pharmaceutically and physiologically acceptable carrier, in an amount effective for use in a medicament, and most preferably for use as a medicament for use in the detection of the presence of infectious organisms in a mammalian host, wherein the pharmaceutical composition is administered to the host, and after allowing a sufficient period of time for the infectious organism to take up the labeled substrate, a determination is made whether the infectious organisms are present in the host by detecting the labeled substrate in the body of the mammalian host.
In accordance with another embodiment, the present invention provides a pharmaceutical composition comprising a labeled substrate, wherein the substrate is a compound that is solely utilized by the metabolism of the infectious organisms selected from the group consisting of prokaryotes, fungi, protozoa, and virally infected host cells; and wherein the label of the labeled substrate is a detectable moiety selected from the group consisting of radionuclides, near-infrared dyes, and fluorescent dyes; wherein the composition includes a pharmaceutically and physiologically acceptable carrier, in an amount effective for use in a medicament, and most preferably for use as a medicament for detection of the location of infectious organisms in the body of a mammalian host, wherein the pharmaceutical composition is administered to the host, and after allowing a sufficient period of time for the infectious organism to take up the labeled substrate, the location of the infectious organisms present in the host is determined by detecting the labeled substrate in the body of the mammalian host.
In accordance with a further embodiment, the present invention provides a pharmaceutical composition comprising a labeled substrate, wherein the substrate is a compound that is solely utilized by the metabolism of the infectious organisms selected from the group consisting of prokaryotes, fungi, protozoa, and virally infected host cells; and wherein the label of the labeled substrate is a detectable moiety selected from the group consisting of radionuclides, near-infrared dyes, and fluorescent dyes; wherein the composition includes a pharmaceutically and physiologically acceptable carrier, in an amount effective for use in a medicament, and most preferably for use as a medicament for determination of the efficacy of an antibiotic therapy in a mammalian host with an infection, wherein the pharmaceutical composition is administered to the host, and after allowing a sufficient period of time for the infectious organism to take up the labeled substrate, the amount infectious organisms present in the host are determined by detecting the labeled substrate in the body of the mammalian host, followed by subjecting the mammalian host to antibiotic treatment for a selected period of time; repeating the administration of the pharmaceutical composition and after allowing a sufficient period of time for the infectious organism to take up the labeled substrate, the amount infectious organisms present in the host are again determined by detecting the labeled substrate in the body of the mammalian host; and assessing whether the infection has been reduced after treatment with the antibiotic by comparing the amount of labeled substrate present before treatment to the amount of labeled substrate present after treatment, wherein when the amount of labeled substrate present after treatment is less, a determination is made that the antibiotic treatment of is effective.
In accordance with an embodiment, the present invention provides a pharmaceutical composition comprising a labeled substrate, wherein the substrate is 18F-fluorodeoxysorbitol ([18F]FDS) and/or derivatives thereof; wherein the composition includes a pharmaceutically and physiologically acceptable carrier, in an amount effective for use in a medicament, and most preferably for use as a medicament for use in the detection of the presence of gram negative organisms in a mammalian host, wherein the pharmaceutical composition is administered to the host, and after allowing a sufficient period of time for the gram negative organism to take up the labeled substrate, a determination is made whether the gram negative organisms are present in the host by detecting the labeled substrate in the body of the mammalian host.
In some other embodiments, the medicament further comprises a pharmaceutically acceptable carrier.
In a further embodiment, the medicament further comprises a second therapeutic agent. In some embodiments, the therapeutic agent is an anti-infective agent, such as antihelmintics, antianaerobics, antibiotics, aminoglycoside antibiotics, antifungal antibiotics, cephalosporin antibiotics, macrolide antibiotics, miscellaneous antibiotics, penicillin antibiotics, quinolone antibiotics, sulfonamide antibiotics, tetracycline antibiotics, antimycobacterials, antituberculosis antimycobacterials, antiprotozoals, antimalarial antiprotozoals, antiviral agents, anti-retroviral agents, scabicides, and urinary anti-infectives.
A therapeutic agent and a biologically active agent are used interchangeably herein to refer to a chemical or biological compound that induces a desired pharmacological and/or physiological effect, wherein the effect may be prophylactic or therapeutic. The terms also encompass pharmaceutically acceptable, pharmacologically active derivatives of those active agents specifically mentioned herein, including, but not limited to, salts, esters, amides, prodrugs, active metabolites, analogs and the like. When the terms “active agent,” “pharmacologically active agent” and “drug” are used, then, it is to be understood that the invention includes the active agent per se as well as pharmaceutically acceptable, pharmacologically active salts, esters, amides, prodrugs, metabolites, analogs etc. The active agent can be a biological entity, such as a virus or cell, whether naturally occurring or manipulated, such as transformed.
In accordance with an embodiment of the present invention, the amount of time of exposure of the labeled substrates to the infectious organisms in the subject should be sufficiently long to effect uptake of the labeled substrate in the infectious organisms in the subject. The time for the desired effect varies with dosage, target, age and other factors known to those of skill in the art. Generally, the time of exposure of the labeled substrates to the infectious organisms should range from about 1 hour to about 120 hours, preferably from about 1 hour to about 24 hours, more preferably from about 1 hour to about 12 hours.
With respect to labeled substrates described herein, the carrier can be any of those conventionally used, and is limited only by physico-chemical considerations, such as solubility and lack of reactivity with the active compound(s), and by the route of administration. The carriers described herein, for example, vehicles, adjuvants, excipients, and diluents, are well-known to those skilled in the art and are readily available to the public. It is preferred that the carrier be one which is chemically inert to the active agent(s), and one which has little or no detrimental side effects or toxicity under the conditions of use. Examples of the carriers include soluble carriers such as known buffers which can be physiologically acceptable (e.g., phosphate buffer) as well as solid compositions such as solid-state carriers or latex beads.
The carriers or diluents used herein may be solid carriers or diluents for solid formulations, liquid carriers or diluents for liquid formulations, or mixtures thereof.
Solid carriers or diluents include, but are not limited to, gums, starches (e.g., corn starch, pregelatinized starch), cellulosic materials (e.g., microcrystalline cellulose), acrylates (e.g., polymethylacrylate), calcium carbonate, magnesium oxide, talc, or mixtures thereof.
For liquid formulations, pharmaceutically acceptable carriers may be, for example, aqueous or non-aqueous solutions, or suspensions. Examples of non-aqueous solvents are propylene glycol, polyethylene glycol, and injectable organic esters such as ethyl oleate. Aqueous carriers include, for example, water, alcoholic/aqueous solutions, cyclodextrins, emulsions or suspensions, including saline and buffered media.
Parenteral vehicles (for subcutaneous, intravenous, intraarterial, or intramuscular injection) include, for example, sodium chloride solution, Ringer's dextrose, dextrose and sodium chloride, lactated Ringer's and fixed oils. Formulations suitable for parenteral administration include, for example, aqueous and non-aqueous, isotonic sterile injection solutions, which can contain anti-oxidants, buffers, bacteriostats, and solutes that render the formulation isotonic with the blood of the intended recipient, and aqueous and non-aqueous sterile suspensions that can include suspending agents, solubilizers, thickening agents, stabilizers, and preservatives.
Intravenous vehicles include, for example, fluid and nutrient replenishers, electrolyte replenishers such as those based on Ringer's dextrose, and the like. Examples are sterile liquids such as water and oils, with or without the addition of a surfactant and other pharmaceutically acceptable adjuvants. In general, water, saline, aqueous dextrose and related sugar solutions, and glycols such as propylene glycols or polyethylene glycol are preferred liquid carriers, particularly for injectable solutions.
The choice of carrier will be determined, in part, by the particular labeled substrate, as well as by the particular method used to administer the composition. Accordingly, there are a variety of suitable formulations of the pharmaceutical composition of the invention. The following formulations for parenteral, subcutaneous, intravenous, intramuscular, intraarterial, intrathecal and interperitoneal administration are exemplary, and are in no way limiting. More than one route can be used to administer the labeled substrates of the present invention, and in certain instances, a particular route can provide a more immediate and more effective response than another route.
Injectable formulations are in accordance with the invention. The requirements for effective pharmaceutical carriers for injectable compositions are well-known to those of ordinary skill in the art (see, e.g., Pharmaceutics and Pharmacy Practice, J.B. Lippincott Company, Philadelphia, Pa., Banker and Chalmers, eds., pages 238-250 (1982), and ASHP Handbook on Injectable Drugs, Trissel, 15th ed., pages 622-630 (2009)).
As used herein the term “pharmaceutically active compound” or “therapeutically active compound” means a compound useful for the treatment or modulation of a disease or condition in a subject suffering therefrom. Examples of pharmaceutically active compounds can include any drugs known in the art for treatment of disease indications. A particular example of a pharmaceutically active compound is an antibiotic agent.
The term “antibiotic agent” as well as words stemming therefrom, as used herein, generally includes pharmaceutically or therapeutically active compounds that work by interfering with the metabolism of the infectious organism. Antibacterial antibiotics are commonly classified based on their mechanism of action, chemical structure, or spectrum of activity. Most target bacterial functions or growth processes. Those that target the bacterial cell wall (penicillins and cephalosporins) or the cell membrane (polymixins), or interfere with essential bacterial enzymes (quinolones and sulfonamides) have bactericidal activities. Those that target protein synthesis (aminoglycosides, macrolides, and tetracyclines) are usually bacteriostatic. Further categorization is based on their target specificity. “Narrow-spectrum” antibacterial antibiotics target specific types of bacteria, such as gram-negative or gram-positive bacteria, whereas broad-spectrum antibiotics affect a wide range of bacteria.
In accordance with an embodiment, the antibiotic agents used in the methods of the present invention may include other drugs effective against fungi, protozoans and antivirals.
For purposes of the invention, the amount or dose of the labeled substrates of the present invention that is administered should be sufficient to effectively target the infectious organisms in vivo, such that the uptake of the labeled substrates can be detected, in the subject over a reasonable time frame. The dose will be determined by the efficacy of the particular labeled substrate formulation and the location of the infectious organisms in the subject, as well as the body weight of the subject to be treated.
The dose of the labeled substrates of the present invention also will be determined by the existence, nature and extent of any adverse side effects that might accompany the administration of a particular labeled substrate. Typically, an attending physician will decide the dosage of the labeled substrates with which to treat each individual subject, taking into consideration a variety of factors, such as age, body weight, general health, diet, sex, compound to be administered, route of administration, and the severity of the condition being treated. By way of example, and not intending to limit the invention, the dose of the labeled substrates of the present invention can be about 0.001 to about 1000 mg/kg body weight of the subject being treated, from about 0.01 to about 100 mg/kg body weight, from about 0.1 mg/kg to about 10 mg/kg, and from about 0.5 mg to about 5 mg/kg body weight. In another embodiment, the dose of the labeled substrates of the present invention can be at a concentration from about 1 nM to about 10,000 nM, preferably from about 10 nM to about 5,000 nM, more preferably from about 100 nM to about 500 nM.
In another embodiment, the term “administering” means that at least one or more labeled substrates of the present invention are introduced into a subject, preferably a subject receiving treatment for a disease, and the at least one or more labeled substrates are allowed to come in contact with the one or more disease related infectious organisms in vivo.
As used herein, the term “treat,” as well as words stemming therefrom, includes diagnostic and preventative as well as disorder remitative treatment.
As used herein, the term “mammalian host” or “subject” refers to any mammal, including, but not limited to, mammals of the order Rodentia, such as mice and hamsters, and mammals of the order Logomorpha, such as rabbits. It is preferred that the mammals are from the order Carnivora, including Felines (cats) and Canines (dogs). It is more preferred that the mammals are from the order Artiodactyla, including Bovines (cows) and Swines (pigs) or of the order Perssodactyla, including Equines (horses). It is most preferred that the mammals are of the order Primates, Ceboids, or Simoids (monkeys) or of the order Anthropoids (humans and apes). An especially preferred mammal is the human.
In a further embodiment, the labeled substrates of the present invention can be used in combination with one or more additional therapeutically active agents which are known to be capable of treating conditions or diseases discussed above. For example, the described labeled substrates of the present invention could be used in combination with one or more known therapeutically active agents, to treat a disease or condition.
As used herein, the term “detection” “imaging” or “radiodetection” means the use of certain properties of isotopes and the energetic particles emitted from radioactive material to diagnose or treat various medical conditions. In addition, the term “scintigraphy” means a diagnostic test in which a two-dimensional image of a body having a radiation source is obtained through the use of radioisotopes. A radioactive chemical is injected intravenously into the patient which then concentrates in the target cells or organ of interest. By placing a camera that senses radioactivity over the body, an image of the target cells or organ of interest can be created. The particles can be detected by suitable devices such as gamma cameras, positron emission tomography (PET) machines, single photon emission computed tomography (SPECT) machines and the like.
In accordance with some embodiments, the term “imaging” or “detection” can also include detection of photons as from a fluorescent dye on the labeled substrate.
In accordance with another embodiment, the present invention provides a method for detection of the location of infectious organisms in the body of a mammalian host comprising: a) administering to the host a therapeutically effective amount of a compound comprising a labeled substrate, wherein the substrate is a compound that is solely utilized by the metabolism of the infectious organisms selected from the group consisting of prokaryotes, fungi, protozoa, and virally infected host cells; and wherein the label of the labeled substrate is a detectable moiety selected from the group consisting of radionuclides, near-infrared dyes, and fluorescent dyes; b) allowing a sufficient period of time for the infectious organism to take up the labeled substrate; and c) determining the location of the infectious organisms present in the host by detecting the labeled substrate in the body of the mammalian host.
In accordance with an embodiment, the present invention provides a method of diagnosing, locating, and assessing the efficacy of treatment of an infectious disease in a patient comprising administering to a subject suspected of having said disease, a labeled substrate prepared according to the above methods, which is selectively taken up by an infectious organism, obtaining a diagnostic image of the subject, determining the location of labeled substrate taken up by the infectious organisms in the subject, and correlating the location of the sequestered labeled substrate with the location of the infectious organisms in the subject. When the detectable moiety is a positron emitter, such as 18F, the spectroscopy can be, for example, SPECT, PET, gamma scintigraphy, or MRI.
In accordance with a further embodiment, the present invention provides a method for the determination of the efficacy of an antibiotic therapy in a mammalian host with an infection comprising: a) administering to the host a therapeutically effective amount of a compound comprising a labeled substrate, wherein the substrate is a compound that is solely utilized by the metabolism of the infectious organisms selected from the group consisting of prokaryotes, fungi, protozoa, and virally infected host cells; and wherein the label of the labeled substrate is a detectable moiety selected from the group consisting of radionuclides, near-infrared dyes, and fluorescent dyes; b) allowing a sufficient period of time for the infectious organism to take up the labeled substrate; c) determining the amount infectious organisms are present in the host by detecting the labeled substrate in the body of the mammalian host; d) subjecting the mammalian host to antibiotic treatment for a selected period of time; e) repeating steps a)-c) one or more times; f) assessing whether the infection has been reduced after d) by comparing the amount of labeled substrate present before d) to the amount of labeled substrate present after d), wherein when the amount of labeled substrate present after d) is less, than a determination is made that the antibiotic treatment of d) is effective.
As used herein, the term “identification” with respect to the inventive methods means that certain species of infectious organisms will preferentially uptake specific labeled substrates while not taking up other labeled substrates, thus allowing one of ordinary skill in the art to deduce the genus and/or species of infectious organism when administering to a subject at least two different labeled substrates. In accordance with one or more embodiment, three, four, five or more different labeled substrates can be administered to a subject and the amount of uptake, based on radiodetection, or fluorescence or another signal, will allow one of skill to make a determination of the species of infections organism infecting the subject.
In accordance with another embodiment of the present invention, labeled D-xylose, in contrast, represents the first molecular probe with the capacity to differentiate between gram negative and gram positive bacteria, with selective uptake only into the latter.
In accordance with yet another embodiment, the present invention provides a method for identification of infectious prokaryotic organisms in a mammalian host comprising: a) administering to the host a therapeutically effective amount of two or more different compounds comprising a labeled substrate, wherein the substrate is a compound that is solely utilized by the metabolism of the infectious organisms selected from the group consisting of prokaryotes, fungi, protozoa, and virally infected host cells; and wherein the label of the labeled substrate is a detectable moiety selected from the group consisting of radionuclides, near-infrared dyes, and fluorescent dyes; b) allowing a sufficient period of time for the infectious organism to take up the labeled substrate; and c) determining the identity of infectious prokaryotic organisms present in the host by detecting and quantifying the amount of each of the labeled substrates in the body of the mammalian host and comparing the quantities of each labeled substrate to a control for the specific species of prokaryotic organisms.
The Enterobacteriaceae is a family of rod-shaped gram-negative bacteria that normally inhabit the gastrointestinal tract and is a frequent source of community and hospital acquired infections. Notable examples include pathogens such as Escherichia coli, Yersinia pestis, Klebsiella pneumoniae and Enterobacter species producing illnesses ranging from pneumonia, meningitis, sepsis, peritonitis, pyelonephritis, etc. Patient mortality approaches 50% in cases of sepsis associated with carbapenem-resistant enterobacteriaceae (CRE). When the source of infection is not clear, noninvasive diagnostic tools are needed to spatially localize and quantify bacteria. The source of infection may thereby be identified and the success of an intervention monitored. 2-[18F]-Fluorodeoxyglucose ([18F]FDG) positron emission tomography (PET) has emerged as a sensitive, noninvasive approach to detect metabolically active oncologic, rheumatic or infectious processes, but cannot reliably differentiate one process from the other. In accordance with one or more embodiments, the present inventors have developed 2-[18F]-fluorodeoxysorbitol ([18F]FDS) as a specific imaging probe for infection by gram negative enterobacteria. Sorbitol is a sugar alcohol approved by the Food and Drug Administration (FDA) as a surgical irrigant and “sugar free” sweetener, yet is metabolized by prokaryotic-specific pathways on selective media. In the examples disclosed herein, commercially available [18F]FDG was rapidly (<30 minutes) converted into [18F]FDS, and which then specifically visualized E. coli myositis and K. pneumoniae pulmonary infection by PET. The technology of the present invention is readily translatable from animal models to humans for clinical use including patients undergoing anticancer treatment with bacterial infections due to Enterobacteriaceae.
In accordance with an embodiment, the present invention provides a specific gram negative prokaryotic organism substrate which is useful in the differentiation of gram positive from gram negative infection in a host. As shown herein, the 18F labeled SOR substrate fluorodeoxysorbitol (([18F]FDS) and derivatives thereof, is actively taken up by gram negative organisms and not taken up by gram positive organisms. Labeling sorbitol with 18F (to produce ([18F]FDS) can be utilized as a means of differentiating gram negative infection from gram positive infection in addition to localizing and quantifying infectious disease burden. This substrate would therefore have an impact on the clinical decision to begin empiric antibiotic therapy as well as the choice of antibacterial agents. In another embodiment, SOR could be labeled with 11C.
[18F]FDS represents the first radio-probe specific for Enterobacteriaceae. Clinically, the probe has numerous applications since the selection of antibiotics is roughly divided by activity against gram positive and negative organisms. Treatment is given empirically while diagnostic tests are performed. This process is invasive, labor intensive, time consuming, and subject to incorrect sampling and contamination. Rapid source control and pathogen identification can guide antibiotic choice and limit drug toxicity. Repeat imaging can monitor treatment efficacy, or suggest the presence of a drug resistant infection, of which the Enterobacteriaceae are notorious.
Carbapenem-resistant Enterobacteriaceae is a serious public health threat which has spread from hospital to community settings. Because CRE are resistant to many classes of antibiotics, achieving source control is essential to successful therapy. Noninvasive, whole host imaging provides an important tool to localize and monitor infection. Even the timely administration of antibiotics with in vitro activity against carbapenem-resistant K. pneumoniae was not associated with patient survival. Removal of the focus of infection (i.e., debridement) was independently associated with patient survival (P=0.002).
The minimal background signal makes the probes of the present invention attractive candidates for visualizing infection in privileged, difficult to sample compartments, such as the brain. The high signal to noise ratio is particularly promising at the high bacterial burden tested, but future studies will aim to decrease the limit of bacilli detected. Since [18F]FDG-PET imaging is widely available, [18F]FDS-PET is readily translatable from animal models to humans for clinical use.
The chemicals used in the study were all purchased from commercial vendors and were used without further purification except where stated. 2-[18F]-Fluorodeoxyglucose was purchased from PETNET Solutions Inc. (Philadelphia, Pa.). Chemical and radiochemical purities were determined by an analytical HPLC system equipped with a Phenomenex Rezex RCM-Monosaccharide Ca++ (8%) Column and both UV and radioactivity detectors. Structure was confirmed by 1H, 13C NMR and mass spectrometry. Sorbitol concentration was measured by a commercial colormetric assay (BioVision, Milpitas, Calif.).
In vitro uptake assays: Freeze dried reference strains of various bacterial species were purchased from American Type Culture Collection (Manassas, Va.) and grown to mid-log phase (OD600 1.0) in Lysogeny Broth (LB) or Middlebrook 7H9 broth supplemented with 10% oleic acid-albumin-dextrose-catalase (Difco, Detroit, Mich.) and 0.05% Tween 80 (Sigma). The number of colony forming units (CFUs) were enumerated by dilution and plating onto solidified media. Tracer uptake assays were performed by incubating bacterial cultures with 7.4 kBq per ml culture of tracer at 37° C. with rapid agitation for 4 hours, with sampling at 0, 30, 120, and 240 minutes. As a control, heat killed (90° C. for 20 minutes) bacteria were similarly incubated with each tracer. Bacteria were pelleted by centrifugation and washed three times with PBS. The activity for each pellet was measured using an automated gamma counter (1282 Compugamma CS Universal gamma counter, LKB Wallac). Background counts were subtracted from sample counts. Six independent replicates were used for each tracer, and for each bacterial strain. Uptake was expressed as the percentage of total tracer added to the culture that resided within the cell pellet.
A commercial library of over 400 random 14C and 3H radio-labeled small molecules (metabolites for nucleic acids, amino acids, lipid synthesis, antibiotics, cofactors, etc) were screened from Moravek Biochemicals, Inc. (Brea, Calif.). Each compound was scored by the following selection criteria: 1) metabolized by prokaryote specific pathways, 2) evidence for prokaryote accumulation or antimicrobial activity, and 3) absence of known eukaryotic accumulation or metabolism. A score +1 or −1 for passing or failing (0 if information unavailable), was assigned to each molecule and for each selection criteria.
In vivo infection. 5-6 week female CBA/J mice (n=3 per group) were immunosuppressed with cyclophosphamide as described by Zuluaga et al. (BMC Infect Dis, 2006. 6: p. 55), then injected with 1×107 E. coli in the right thigh and either 50 μg LPS, heat inactivated E. coli, or live S. aureus in the left thigh. For heat inactivation, the live culture was placed on wet ice and an aliquot was subjected to a 90° C. water bath for 20 minutes. For a pneumonia model, 3×106 K. pneumoniae were instilled into the mainstream bronchus of sedated, neutropenic mice as described by Rouse et al (Animal Models of Gram-negative Bacillary Experimental Pneumonia, in Handbook of animal models of infection, O. Zak and M. A. Sande, Editors. 1999, Academic Press: London, UK. p. 495-500). The night before each imaging time-point, mice were fasted for 12 hours. Water was provided ad libitum. 7.4 MBq [18F]FDS was injected by tail vein 2 hours prior to collecting a static 15 minute frame injection using a Mosaic HP (Philips) Small Animal PET. [18F]FDG was injected 45 minutes prior to data collection as described by Davis et al (Antimicrob Agents Chemother, 2009. 53(11): p. 4879-84). CT scans were performed at the same time with a NanoSPECT/CT (BIOSCAN) in vivo animal imager. PET data were reconstructed and co-registered with CT images. Mice were sacrificed to collect tissues for direct gamma counting and to determine the number of bacilli implanted. The thighs were homogenized in PBS and plated onto solidified LB media overnight at 37° C. to enumerate the colony forming units (CFU). At least 3 mice were used for each group.
Synthesis of 18F-fluorodeoxysorbitol ([18F]FDS). The novel gram-negative bacteria-specific imaging biomarker [18F]FDS can be synthesized rapidly and easily at very low cost using the most commonly used PET tracer 18F-fluorodeoxyglucose, which is commercially available all over the world. Previously proposed tracers either require complex chemical synthesis or utilize expensive radioisotopes (e.g. 1-124 in FIAU) which could limit their widespread use. However, [18F]FDS can be rapidly (<30 min) synthesized from [18F]FDG using a low cost technique (Li Z B, et al., Mol. Imaging Biol., 2008; 10:92-8) and is shown in
[18F]FDS uptake and imaging. In vitro uptake assays exposed bacterial cultures to 7.4 kBq per ml [18F]FDS over 2 hours, then cells were pelleted, washed and gamma counted. Intracellular uptake assays were performed at least in triplicate and indicate the percent of the total radioactivity (added to the culture), that was found in the bacterial pellet (after several steps of washing). Heat-killed bacteria were used as negative controls and no uptake was noted in any species. For in vivo studies, 5-6 week female CBA/J mice (n=3 per group) were immunosuppressed with cyclophosphamide then innoculated with bacteria as specified. 7.4 MBq [18F]FDS or [18F]FDG was injected by tail vein 2 hours prior to collecting a static 15 minute frame for myositis imaging. For pulmonary infection imaging, 18.5 MBq [18F]FDS was injected, and images were collected by dynamic windows of 15 minutes over the course of 180 minutes. CT scans were performed for coregistration with PET images. Tissues were collected for direct gamma counting.
For semiquantitative analysis, 1-2 spherical (3 mm diameter) regions of interest (ROI) were drawn manually in the thighs using CT as a guide of each animal. The standard uptake values (SUV) were computed by normalizing the ROI activity for each mouse to the injected dose and animal weight using Amide version 0.9.1 (amide.sourceforge.net). For each group, the mean PET activity at each time-point was calculated by averaging the normalized thigh SUVs of all the ROIs in that group. Amira version 5.4.2 (amira.com) was used to visualize the images.
All protocols were approved by the Johns Hopkins Biosafety, Radiation Safety and Animal Care and Use Committees.
Statistical analysis. Statistical comparison between groups was performed using one tail distribution, two sample, unequal variance t-test in Excel 2007 (Microsoft). Data are presented on a linear scale as mean±standard error for the mean PET activities.
Seven (3%) compounds (
A mycobacterial lead compound (PABA) was also evaluated in Mycobacterium tuberculosis and demonstrated significant (96.3% at 18-hours) and rapid (32.9% at 2-hours) accumulation. No significant accumulation was noted in host-cells (J774 macrophages) at the corresponding time-points, indicating that the uptake is bacteria-specific (
[14C] D-mannitol was another of the promising molecules identified by the methods of the present invention. It accumulated rapidly and in significant amounts in all three models bacteria tested. Intracellular uptake was 66%, 82% and 83% in S. aureus, E. coli and M. smegmatis respectively after only 2 hours of incubation (
Differentiation of microbes by selective growth media is a mainstay of clinical microbiology practice, such as MacConkey agar supplemented with sorbitol to isolate pathogenic E. coli. To identify infection in vivo, we extended this approach to selectively label Enterobacteriaceae with positron emitting fluorodeoxysorbitol. We efficiently converted commercially available [18F]FDG to [18F]FDS by chemical reduction in less than 30 minutes. We first tested cultures of E. coli K12 and K. pneumoniae to assess accumulation of the probe in vitro (
To further assess the specificity of uptake, E. coli cultures were co-incubated with [18F]FDS and increasing concentrations of unlabeled sorbitol (
In-vivo imaging using [18F]FDS. We next investigated whether [18F]FDS-PET was capable of detecting infection with E. coli in vivo Immunosuppressed CBA/J mice were inoculated with either 1.49×107 CFU of live E. coli (
To next test the selectivity of FDS in the presence of mixed infections, immunosuppressed CBA/J mice were inoculated with 7.6×106 CFU live E. coli (right thigh) and 1.5×109 CFU live S. aureus (left thigh). These mice were considerably more ill-appearing due to the added infectious burden of S. aureus, prompting a shorter incubation time of 6 hours prior to imaging. [18F]FDS yielded significantly more intensity at the site of the E. coli infection in the left thigh (
We next examined whether [18F]FDS could detect pulmonary infection associated with K. pneumoniae, the causative agent of Friedlander's disease Immunosuppressed CBA/J mice inoculated with 3×106 CFU by intratracheal instillation developed pulmonary infiltrates observed by CT over the course of 5 days of incubation. As expected, the areas of lung infiltration coregistered with foci of PET signal intensity (
In order to confirm the feasibility of 18F labeled PABA for detection of microorganisms using PET imaging, fluorinated derivatives of PABA labeled with 3H were used to test for intracellular accumulation in S. aureus, E. coli, and M. tuberculosis.
Mycobacterium tuberculosis cultures were incubated with [3H]-PABA, [3H]-2-F-PABA and [3H]-3-F-PABA according to the protocols in the previous examples. High intracellular uptake was found in M. tb after 18 hours of incubation: PABA (58.5%), 2-F-PABA (29.4%) and 3-F-PABA (11.2%). In addition, there was no significant intracellular uptake (<1%) of PABA, 2-F-PABA or 3-F-PABA in eukaryotic J774 macrophages, Human Brain Microvascular Endothelial Cells (HBMEC) and WEHI 164 fibroblasts.
Furthermore, preliminary data on [3H]-PABA, [3H]-2-F-PABA uptake in various bacterial pathogens gave positive results (>5% of selective intracellular uptake) for Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Enterobacter cloacae, Enterococcus faecalis, Streptococcus pyogenes and Serratia marcescens.
We next investigated whether [3H]-2-F-PABA was capable of detecting infection with S. aureus in vivo. Immunosuppressed CBA/J mice were inoculated with either live S. aureus (right thigh) or 50 μg of LPS (left thigh). [3H]-2-F-PABA was injected IV using a tail vein catheter. Mice were sacrificed following injection, tissues were then resected and radiotracer uptake measured by scintillation counting. Measured by this technique, [3H]-2-F-PABA had a right thigh vs. left thigh uptake ratio of 2.62±0.98 (P<0.05). Thus, demonstrating the feasibility of 18F labeled 2-F-PABA for imaging gram positive infections.
In order to test the feasibility of 18F labeled mannitol for detection of gram positive organisms using PET imaging, a fluorinated derivative of mannitol (2-fluoro-mannitol) labeled with 3H was used to test the uptake characteristics of the probe. We first incubated S. aureus for 30 minutes with 5 and 10 mM of glucose in the presence of 14C-mannitol and found an intracellular uptake of 33% and 30% respectively as compared to 53.5% without any glucose in the media. Thus indicating the presence of specific mannitol transporters in S. aureus.
An in silico analysis for genes encoding mannitol specific transporters was performed, with positive results for multiple bacteria including S. aureus, E. coli, S. mutans, suggesting that 2-fluoromannitol can detect a broad range of clinically significant pathogens
Experiments were then performed showing intracellular uptake of [3H]-2-F-mannitol after 120 minutes of incubation was 8.98%. Moreover, it was found that if [3H]-2-F-mannitol was incubated with 0.2 mM of cold mannitol amplified its intracellular uptake to 31.08% due to the induction of sugar transporters. Mannitol is an FDA approved diuretic, and the dose required for diuresis is similar the dose observed to amplify 2-Fluoromannitol uptake. Thus, 18F labeled 2-F-mannitol is an interesting probe for imaging infections that may prove to be extremely sensitive for detecting small numbers of microbes.
All references, including publications, patent applications, and patents, cited herein are hereby incorporated by reference to the same extent as if each reference were individually and specifically indicated to be incorporated by reference and were set forth in its entirety herein.
The use of the terms “a” and “an” and “the” and similar referents in the context of describing the invention (especially in the context of the following claims) are to be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. The terms “comprising,” “having,” “including,” and “containing” are to be construed as open-ended terms (i.e., meaning “including, but not limited to,”) unless otherwise noted. Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., “such as”) provided herein, is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention unless otherwise claimed. No language in the specification should be construed as indicating any non-claimed element as essential to the practice of the invention.
Preferred embodiments of this invention are described herein, including the best mode known to the inventors for carrying out the invention. Variations of those preferred embodiments may become apparent to those of ordinary skill in the art upon reading the foregoing description. The inventors expect skilled artisans to employ such variations as appropriate, and the inventors intend for the invention to be practiced otherwise than as specifically described herein. Accordingly, this invention includes all modifications and equivalents of the subject matter recited in the claims appended hereto as permitted by applicable law. Moreover, any combination of the above-described elements in all possible variations thereof is encompassed by the invention unless otherwise indicated herein or otherwise clearly contradicted by context.
This application claims the benefit of U.S. Provisional Patent Application Nos. 61/701,041, filed on Sep. 14, 2012, and 61/765,925, filed on Feb. 18, 2013, both of which are hereby incorporated by reference for all purposes as if fully set forth herein.
This invention was made with U.S. government support under grant no. DP2OD006492-01 awarded by the NIH. The U.S. government has certain rights in the invention.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US2013/059897 | 9/16/2013 | WO | 00 |
| Number | Date | Country | |
|---|---|---|---|
| 61701041 | Sep 2012 | US | |
| 61765925 | Feb 2013 | US |
