Claims
- 1. A transcriptional activating sequence that is selected from the group consisting of: ##STR10## wherein A is deoxyadenyl; G is deoxyguanyl; C is deoxycytidyl; T is thymidyl.
- 2. The transcriptional activating sequence of claim 1 that is ##STR11##
- 3. The transcriptional activating sequence of claim 1 that is ##STR12##
- 4. The transcriptional activating sequence of claim 1 that is ##STR13##
- 5. The transcriptional activating sequence of claim 1 that is ##STR14##
- 6. The transcriptional activating sequence of claim 1 that is ##STR15##
- 7. A recombinant DNA expression vector that comprises a transcriptional activating sequence consisting of a DNA sequence selected from a transcriptional activating sequence of claim 1.
- 8. A recombinant DNA expression vector of claim 7 that sequentially comprises:
- a) said transcriptional activating sequence,
- b) a translational activating sequence, and
- c) a DNA sequence that codes for a functional polypeptide,
- such that a) and b) are positioned for the expression of c).
- 9. A recombinant DNA expression vector of claim 8 that is a plasmid.
- 10. The plasmid of claim 9 that is pHPR104.
- 11. The plasmid of claim 9 that is pHDM159.
- 12. The plasmid of claim 9 that is pHPR106.
- 13. The plasmid of claim 9 that is pHPR106A.
- 14. The plasmid of claim 9 that is psynD.
- 15. A host cell transformed with the vector of claim 8.
- 16. The host cell of claim 15 that is Escherichia coli.
- 17. The host cell of claim 16 that is Escherichia coli K12 RV308.
- 18. The host cell of claim 17 that is E. coli K12 RV308/pHPR104.
- 19. The host cell of claim 17 that is E. coli K12 RV308/pHDM159.
- 20. The host cell of claim 17 that is E. coli K12 RV308/pHPR106.
- 21. The host cell of claim 17 that is E. coli K12 RV308/pHPR106A.
- 22. The host cell of claim 17 that is E. coli K12 RV308/psynD.
- 23. A method of producing a functional polypeptide comprising: culturing the host cell of claim 16 under conditions that enable the expression of the encoded polypeptide.
- 24. A method of claim 23 wherein the DNA sequence that codes for a functional polypeptide encodes: .beta.-galactosidase, human preproinsulin, human proinsulin, human insulin A-chain, human insulin B-chain, non-human insulin, human growth hormone, insulin-like growth factors, human interferon, viral antigens, urokinase, tissue-type plasminogen activator, interleukins 1-6, colony stimulating factors, erythropoetin, human transferrin, and EK-BGH.
- 25. A method of claim 24 wherein the DNA sequence that codes for a functional polypeptide encodes EK-BGH.
- 26. The recombinant DNA expression vector pHPR97.
Parent Case Info
CROSS REFERENCE TO RELATED APPLICATIONS
This application is a continuation of application Ser. No. 08/000,511, filed on Jan. 4, 1993, now abandoned, which is a continuation of application Ser. No. 07/739,280, filed on Aug. 1, 1991, now abandoned, which is a continuation-in-part of application Ser. No. 07/565,783, filed Aug. 13, 1990, now abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4874703 |
Jaskunas |
Oct 1989 |
|
Foreign Referenced Citations (2)
Number |
Date |
Country |
0058002 |
Aug 1982 |
EPX |
0314274 |
May 1989 |
EPX |
Non-Patent Literature Citations (7)
Entry |
Jones, et al., 1982, Mol. Gen. Genet., 188:486. |
Sarai, 1989, Proc. Natl. Acad. Sci. USA, 86:6513. |
Ensley, B. D., 1986, CRC Crit. Rev. Biotechnol., 4(3):263. |
Kusano, et al., 1989, J. Mol. Biol., 209:623. |
Szardenings, M., et al., 1990, Gene, 94:1-7. |
Szardenings, M., et al., Apr. 9, 1990, Genbank. |
Brandt, M. E., et al., Jul. 11, 1990, Genbank. |
Continuations (2)
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Number |
Date |
Country |
Parent |
000511 |
Jan 1993 |
|
Parent |
739280 |
Aug 1991 |
|
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
565783 |
Aug 1990 |
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