Claims
- 1. A compound of the formula ##STR5## wherein R.sub.1 is halogen or nitro; R.sub.2 is hydrogen or halogen; and R.sub.3 is hydrogen or lower alkyl and n=2-6.
- 2. The compound of claim 1 wherein R.sub.1 is halogen.
- 3. The compound of claim 2 wherein R.sub.2 is hydrogen.
- 4. The compound of claim 3 wherein R.sub.3 is hydrogen.
- 5. The compound of claim 4 wherein n=2.
- 6. The compound of claim 5 wherein R.sub.1 is Cl; said compound having the formula ##STR6##
- 7. The compound of claim 1 which is radiolabelled.
- 8. The compound of claim 7 wherein the compound is radiolabelled with I.sup.125.
- 9. The compound of claim 8 which is radiolabelled with I.sup.125 wherein the compound is I.sup.125 -7-chloro-3-[2-(4-hydroxyphenyl)ethoxy)-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepine-2-one.
- 10. In a method for the radioimmunoassay for a benzodiazepine compound or metabolites thereof in a sample, which comprises mixing said sample with a known amount of a labelled benzodiazepine compound and antibody which selectively bind said benzodiazepine compound and said labelled benzodiazepine compound, measuring the degree of binding of the said labelled benzodiazepine compound to said antibody, and determining the amount of said benzodiazepine compound in said sample by comparing said degree of binding to a standard curve; the improvement which comprises utilizing a radiolabelled compound of the formula ##STR7## wherein R.sub.1 is halogen or nitro; R.sub.2 is hydrogen or halogen; and R.sub.3 is hydrogen or lower alkyl and n=2-6.
- 11. The method of claim 10 wherein R.sub.1 is halogen.
- 12. The method of claim 10 wherein R.sub.2 is hydrogen.
- 13. The method of claim 12 wherein R.sub.3 is hydrogen.
- 14. The method of claim 13 wherein n=2.
- 15. The method of claim 14 wherein R.sub.1 is Cl said compound having the formula ##STR8##
- 16. The method of claim 15 where the compound is radiolabelled with I.sup.125 wherein said compound is I.sup.125 -7-chloro-3-[2-(4-hydroxyphenyl)ethoxy)-5-phenyl-1,-dihydro-2H-1,4-benzodiazepine-2-one.
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation-in-part of U.S. patent application Ser. No. 077,472 filed July 24, 1987.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
3176009 |
Bell |
Mar 1965 |
|
4083948 |
Davis et al. |
Apr 1978 |
|
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
77472 |
Jul 1987 |
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