.beta., .gamma.-Dihydropolyprenyl alcohol and hypotensive pharmaceutical composition containing same

Abstract

.beta., .gamma.-Dihydropolyprenyl alcohols of the general formula: ##STR1## wherein n represents an integer of 8 to 10, exhibit hypotensive activity and anti-hepatic disease-activity.

Description

The present invention relates to 62 , .gamma.-dihydropolyprenyl alcohols of the general formula: ##STR2## wherein n represents an integer of 8 to 10, and a pharmaceutical composition containing the same, useful for treating hypertension and hepatic disease.

Numerous hypotensive drugs have been developed for the treatment of hypertension. However, prior hypotensive drugs have various adverse side effects and, therefore, problems occur in the administration thereof, particularly when they are administered in large amounts or over a long period of time. For example, diuretic hypotensive drugs such as sulfonamide preparations and thiazide preparations have side effects of causing hyper-uricemia and hypo-potassemia; sympatholytic agents such as reserpine preparations and methyl dopa preparations have harmful side effects of causing thirst, clouding of consciousness and orthostatic hypotension; and vasodilator drugs such as apresoline have harmful side effects of causing headache, tachycardia and angina pectoris in many cases. After intensive investigations for the purpose of obtaining safer hypotensive drugs free of those defects, the inventors have discovered compounds of the above formula (I).

The compounds of the formula (I) of the present invention can be produced by a process comprising the following steps:

(a) reacting compounds of the general formula: ##STR3## wherein n represents an integer of 8 to 10, with lower alkyl esters of cyanoacetic acid, in the presence of a base, to form compounds of the general formula: ##STR4## wherein n has the same meaning as set forth above and R represents a lower alkyl group; (b) reducing the compounds of general formula (III) with a reducing agent, such as sodium borohydride, to obtain compounds of the general formula: ##STR5## wherein n and R have the same meanings as set forth above; (c) subjecting the compounds of general formula (IV) to decarboxylation in the presence of a strong alkali, such as potassium hydroxide, to obtain compounds of the general formula: ##STR6## wherein n has the same meaning as set forth above; (d) hydrolyzing the compounds of general formula (V) in the presence of a strong alkali, such as potassium hydroxide, to obtain compounds of the general formula: ##STR7## wherein n has the same meaning as set forth above; and (e) reducing the compounds of general formula (VI) with a reducing agent, such as sodium bis (2-methoxyethoxy) aluminum hydride or lithium aluminum hydride, to obtain compounds of the general formula: ##STR8## wherein n has the same meaning as set forth above.

BRIEF DESCRIPTION OF THE DRAWING

The drawing is a graph showing the changes in the blood pressure and the heart rate of spontaneous hypertension rats (SHR) to which 3, 7, 11, 15, 19, 23, 27, 31, 35, 39-decamethyl-6, 10, 14, 18, 22, 26, 30, 34, 38-tetracontanonaene-1-ol of the present invention was administered.

The pharmacological effects and toxicities (acuate toxicities) of the compounds of the present invention, examined by animal tests, are described below.

Pharmacological tests

I. The hypotensive effect of the formula (I) compound, according to the invention, on spontaneously hypertensive rats (hereinafter referred to as SHR) of Okamoto and Aoki:

Method

The hypotensive effects of the test compounds on SHR of Okamoto and Aoki of about 35 weeks age were determined. The systolic blood pressure of the SHR was around 230 mmHg.

The test compounds were given to the SHR orally in the form of a suspension in aqueous acacia solution. The SHR were divided into a test compound group to which a test compound was administered and a control group to which only an aqueous acacia solution (free of test compound) was administered. Each group comprised three SHR.

The blood pressure of the SHR was measured with a Shimazu continuous tonometer SCS-301 (a product of Shimazu Seisakusho Co., Ltd.). Tail artery pressure was indirectly measured by a tail-cuff method.

The blood pressure was measured immediately before the administration and at 2, 4, 6 and 24 hours after the administration to determine the change in blood pressure with the passing of time.

The heart rate was determined by recording the pulse rate at the tail of the SHR with a tachometer of said tonometer SOS-301. Test Compound and Dose

Test Compound

3, 7, 11, 15, 19, 23, 27, 31, 35, 39-Decamethyl-6, 10, 14, 18, 22, 26, 30, 34, 38-tetracontanonaene-1-ol

Dose

10 mg/kg (body weight of SHR)

Results

The changes in the blood pressure and the heart rate due to the administration of the test compound are shown in the drawing.

It is evident from the drawing that in the control group, the blood pressure before the administration was 232.+-.9 mgHg and it was 232.+-.7 mmHg 6 hours after the administration, the blood pressure thus being substantially unchanged. In the group to which 10 mg/kg of the test compound was given, the blood pressure before the administration was 227.+-.7 mmHg and it lowered to 207.+-.9 mmHg two hours after the administration, further to 202.+-.12 mmHg six hours after the administration and the blood pressure was 212.+-.9 mmHg 24 hours after the administration. Thus, it was recognized that the test compound has a clear, long-lasting hypotensive effect. No effect on the heart rate was observed.

II. The effects of the formula (I) compound, according to the invention, on hepatic disease (hepatitis), induced by intraabdominal administration of D-galactosamine.

SD rats weighing about 250 g were used as test animals. D-Galactosamine hydrochloride (250 mg/kg each administration) and the test compound (50 mg/kg each administration) were administered intraabdominally to the rats on the schedule of experiments set forth below. After completion of the administration, blood was drawn from the test animals. The GPT value and alkali phosphatase value of the blood, which are indexes of the hepatic disease, were determined.

The test compound was used in the form of a suspension in 5% aqueous acacia solution. D-Galactosamine hydrochloride was used in the form of an aqueous solution thereof in distilled water, which solution was adjusted to pH 7 with potassium hydroxide. The rats were divided into three groups, i.e., a test compound group (comprising 9 rats) to which the test compound was administered, a control group (comprising 14 rats) to which 5% aqueous acacia solution free of the test compound was administered and a normal group (comprising 9 rats) to which neither the test compound nor D-galactosamine hydrochloride was administered. ##STR9##

Test compound

3, 7, 11, 15, 19, 23, 27, 31, 35, 39-Decamethyl-6, 10, 14, 18, 22, 26, 30, 34, 38-tetracontanonaene-1-ol:

TABLE 1______________________________________Test Results GPT Value Alkali phosphataseTest groups (Carmen unit) value (KA-U)______________________________________Normal group 46.5 .+-. 4.9 32.3 .+-. 2.5Control group 1041.4 .+-. 200.8 70.9 .+-. 3.9Test compound 523.3 .+-. 111.7 63.7 .+-. 4.3group______________________________________

It is evident from the above table that the group of rats to which the test compound was administered (test compound group) had GPT values and alkali phosphatase values lower than those of the control group and closer to those of the normal group. This fact indicates that hepatic disease induced by the administration of D-galactosamine hydrochloride was ameliorated or prevented by the administration of the compound of the present invention.

Toxicity test

1,000 mg/kg of said test compound was administered orally to SD rats (males and females; about 200 g body weight). Neither death nor toxic side effects were observed.

It is apparent from the results of the above pharmacological tests and the toxical tests that the compounds of formula (I) of the present invention have an excellent effect of lowering the blood pressure and ameliorating hepatic disease induced by administration of D-galactosamine hydrochloride. They are substantially free of toxicity and, therefore, they are quite safe. Thus, the compounds of the formula (I) of the present invention are effective for the prevention and treatment of renal hypertension, endocrine hypertension, cardiovascular hypertension, neuropahtic hypertension and essential hypertension and they are effective for treating hepatic disease. The administration route and dose of the compounds of the formula (I) of the present invention can be selected and controlled suitably depending on the severity of the symptoms to be treated. For oral administration, the dose is 10-200 mg/day, preferably 50-100 mg/day, for adult human beings.

The compounds of the formula (I) of the present invention can be incorporated with conventional, pharmacologically acceptable carriers to prepare unit dosage forms in the form of powders, granules, tablets, capsules and injections by conventional techniques.

The following examples further illustrate the present invention.

EXAMPLE 1

Preparation of 3, 7, 11, 15, 19, 23, 27, 31, 35, 39-decamethyl-6, 10, 14, 18, 22, 26, 30, 34, 38-tetracontanonaene-1-ol:

(a) Preparation of ethyl 2-cyano-3, 7, 11, 15, 19, 23, 27, 31, 35, 39-decamethyl-2, 6, 10, 14, 18, 22, 26, 30, 34, 38-tetracontadecanoate:

35 Grams of nonaprenylacetone (formula II), 9.4 g of ethyl cyanoacetate, 5 g of ammonium acetate and 4 ml of acetic acid were dissolved in 300 ml of benzene. The azeotropic water was removed under reflux of benzene. After refluxing for 5 hours, 500 ml of hexane were added thereto. The mixture was washed with water and concentrated. The concentrate was purified by silica gel column chromatography with hexane-benzene solvent mixture as developer to obtain 30 g of the intended product as a white waxy substance:

Elementary analysis as C.sub.53 H.sub.83 O.sub.2 N:

______________________________________ C H N______________________________________Theoretical (%): 83.08 10.92 1.83Found (%): 83.05 10.89 1.85______________________________________

Mass spectrum: 765 (M.sup.+).

Infrared absorption spectrum (cm.sup.-1): 2950, 2860, 2240, 1735, 1665, 1610, 1455, 1390, 1238, 1100.

Nuclear magnetic resonance spectrum (CDCl.sub.3, ppm); 1.32(t, 3H), 1.60(S, 30H), 1.62(s, 3H), 1.98 (m, 34H), 2.25(m, 2H), 4.25(q, 2H), 5.10(m, 9H).

(b) Preparation of ethyl 2-cyano-3, 7, 11, 15, 19, 23, 27, 31, 35, 39-decamethyl-6, 10, 14, 18, 22, 26, 30, 34, 38-tetracontanonaenoate:

1.3 Grams of sodium borohydride were dissolved in 50 ml of ethanol. The resulting solution was added dropwise to a 15% solution of 25 g of the compound obtained in the above step (a) in dioxane under cooling with ice. After stirring for one hour, 10 ml of saturated aqueous ammonium chloride solution were added dropwise thereto and then 500 ml of n-hexane were added thereto. The organic layer thus separated was taken out, washed with water and then concentrated. The concentrate was purified by silica gel column chromatography with hexane/benzene solvent mixture as developer to obtain 21 g of the intended product as a white waxy substance.

Elementary analysis as C.sub.53 H.sub.85 O.sub.2 N:

______________________________________ C H N______________________________________Theoretical (%): 82.86 11.15 1.82Found (%): 82.83 11.14 1.86______________________________________

Mass spectrum: 767 (M.sup.+).

Infrared absorption spectrum (cm.sup.-1): 2930, 2860, 2260, 1750, 1665, 1450, 1385, 1255, 1195.

Nuclear magnetic resonance spectrum (CCl.sub.4, ppm): 1.15(t, 3H), 1.18(d, 3H), 1.40(m, 2H), 1.60(x, 27H), 1.68(s, 3H), 1.99(m, 34H), 2.13(m, 1H), 3.65(m, 1H), 4.25 (q, 2H), 5.10(m, 9H).

(c) Preparation of 1-cyano-3, 7, 11, 15, 19, 23, 27, 31, 35, 39-decamethyl-6, 10, 14, 18, 22, 26, 30, 34, 38-tetracontanonaene:

2.0 Grams of 85% potassium hydroxide and 13.5 g of the compound obtained in the above step (b) were dissolved in 100 ml of ethylene glycol. The mixture was refluxed under stirring for 24 hours. Then, the reaction mixture was poured into water, neutralized with dilute hydrochloric acid and extracted with hexane. The extract was washed with water and concentrated. The concentrate was purified by silica gel column chromatography with hexane/benzene solvent mixture as developer to obtain 8 g of the intended product as a white waxy substance.

Elementary analysis as C.sub.50 H.sub.81 N:

______________________________________Theoretical (%): C H N______________________________________Theoretical (%): 86.26 11.73 2.01Found (%): 86.23 11.70 2.03______________________________________

Mass spectrum: 695 (M.sup.+).

Infrared absorption spectrum (cm.sup.-1): 2930, 2860, 2250, 1665, 1450, 1385, 1100.

Nuclear magnetic resonance spectrum (CCl.sub.4, ppm): 1.05(d, 3H), 1.45(m, 2H), 1.60(s, 27H), 1.68(s, 3H), 2.00(m, 34H), 2.10(m, 1H), 2.25(d, 2H), 5.13(m, 9H).

(d) Preparation of 3, 7, 11, 15, 19, 23, 27, 31, 35, 39-Decamethyl-6, 10, 14, 18, 22, 26, 30, 34, 38-tetracontanonaenoic acid:

3.8 Grams of 85% potassium hydroxide and 8.0 g of the compound obtained in the above step (c) were dissolved in 100 ml of propylene glycol. The resulting solution was refluxed under stirring for 18 hours. Then, the reaction mixture was poured into water, neutralized with dilute hydrochloric acid and extracted with hexane. The extract was washed with water and concentrated. The concentrate was purified by silica gel column chromatography with hexane/benzene solvent mixture as developer to obtain 3.0 g of the intended product as a white waxy substance.

Elementary analysis as C.sub.50 H.sub.82 O.sub.2 :

______________________________________ C H______________________________________Theoretical (%): 83.97 11.56Found (%): 83.96 11.56______________________________________

Mass spectrum: 714 (M.sup.+).

Infrared absorption spectrum (cm.sup.-1): 2500-3400, 2930, 2860, 1710, 1665, 1455, 1385, 1300, 1100.

Nuclear magnetic resonance spectrum (CCl.sub.4, ppm): 1.00(d, 3H), 1.45(m, 2H), 1.64(s, 27H), 1.70(s, 3H), 2.00(m, 1H), 2.04(m, 34H), 2.24(d, 2H), 5.16 (m, 9H), 11.28(bs, 1H).

(e) Preparation of 3, 7, 11, 15, 19, 23, 27, 31, 35, 39-Decamethylene-6, 10, 14, 18, 22, 26, 30, 34, 38-tetracontanonaene-1-ol:

3.0 Grams of the compound obtained in the above step (d) were dissolved in 20 ml of benzene. 5.0 Grams of 70% solution of vitride in benzene were added dropwise thereto. After stirring for one hour, water was added to the mixture to decompose the excess sodium bis (2-methoxyethoxy)aluminium hydride.

The resulting precipitates were filtered out. 50 millileters of acetone were added to the precipitates and the whole was refluxed and filtered. Acetone was distilled off from the filtrate. The resulting concentrate was combined with the former filtrate, washed with water and concentrated. The concentrate was purified by silica gel column chromatography with n-hexane/benzene solvent mixture as developer to obtain 1.6 g of the intended product as a white waxy substance.

Elementary analysis as C.sub.50 H.sub.84 O:

______________________________________ C H______________________________________Theoretical (%): 85.64 12.08Found (%): 85.63 12.11______________________________________

Mass spectrum: 700 (M.sup.+).

Infrared absorption spectrum (cm.sup.-1): 3450, 2930, 2860, 1665, 1450, 1385, 1105, 1060.

Nuclear magnetic resonance spectrum (CCl.sub.4, ppm): 0.95(d, 3H), 1.20(m, 1H), 1.23-1.55(m, 4H), 1.58(s, 27H), 1.66(s, 3H), 1.96(bs, 1H), 2.00 (m, 34H), 3.66(t, 2H), 5.15(m, 9H).

EXAMPLE 2

3, 7, 11, 15, 19, 23, 27, 31, 35-Nonamethyl-6, 10, 14, 18, 22, 26, 30, 34-hexatriacontaoctaene-1-ol:

The intended product in the form of a white waxy substance was prepared starting with octaprenylacetone in the same manner as described in Example 1.

Elementary analysis as C.sub.45 H.sub.76 O:

______________________________________ C H______________________________________Theoretical (%): 85.37 12.10Found (%): 85.38 12.10______________________________________

Mass spectrum: 632 (M.sup.+).

Infrared absorption spectrum (cm.sup.-1): 3450, 2930, 2860, 1665, 1450, 1385, 1105, 1060.

Nuclear magnetic resonance spectrum (CCl.sub.4, ppm): 0.97 (d, 3H), 1.23 (m, 1H), 1.25-1.57 (m, 4H), 1.60 (s, 24H), 1.68 (s, 3H), 2.00 (bS, 1H), 2.03 (m, 30H), 3.68 (t, 2H), 5.17 (m, 8H).

EXAMPLE 3

3, 7, 11, 15, 19, 23, 27, 31, 35, 39, 43-Undecamethyl-6, 10, 14, 18, 22, 26, 30, 34, 38, 42-tetratetracontadecaene-1-ol:

The intended product in the form of a white waxy substance was prepared starting with decaprenylacetone in the same manner as described in Example 1.

Elementary analysis as C.sub.55 H.sub.92 O:

______________________________________ C H______________________________________Theoretical (%): 85.87 12.05Found (%): 85.86 12.03______________________________________

Infrared absorption spectrum (cm.sup.-1): 3450, 2930, 2860, 1665, 1450, 1385, 1105, 1060.

Nuclear magnetic resonance spectrum (CCl.sub.4, ppm): 0.95 (d, 3H), 1.21 (m, 1H), 1.25-1.58 (m, 4H), 1.59 (s, 30H), 1.68 (s, 3H), 1.94 (bs, 1H), 2.01 (m, 38H), 3.68 (t, 2H), 5.16 (m, 10H).

EXAMPLE 4 CAPSULES

______________________________________Principal ingredient (Compound of Example 1) 5 gMicrocrystalline cellulose 80Corn starch 20Lactose 22Polyvinylpyrrolidone 3Total 130 g______________________________________

The above components were shaped into granules by a conventional method. The granules were charged in 1,000 gelatin hard capsules. Each capsule contained 5 mg of the principal ingredient.

EXAMPLE 5 POWDER

______________________________________Principal ingredient (Compound of Example 1) 50 gMicrocrystalline cellulose 400Corn starch 550Total 1,000 g______________________________________

The principal ingredient was dissolved in acetone. The solution was adsorbed on microcrystalline cellulose and then the whole was dried. The dry product was mixed with corn starch to obtain a powder by a conventional method. Thus, the powder containing the principal ingredient of 1/20 strength was obtained.

EXAMPLE 6 TABLETS

______________________________________Principal ingredient (Compound of Example 2) 5 gCorn starch 10Lactose 20Calcium carboxymethyl cellulose 10Microcrystalline cellulose 40Polyvinylpyrrolidone 5Talc 10Total 100 g______________________________________ The principal ingredient was dissolved in acetone. The solution was adsorbed on microcrystalline cellulose and the whole was dried. The dry product was mixed with corn starch, lactose and calcium carboxymethyl cellulose. Then, an aqueous polyvinylpyrrolidone solution was added thereto as a binder. The mixture was shaped into granules by a conventional method. Talc as lubricant was then mixed therein and the mixture was shaped into tablets, each weighing 100 mg. Each tablet contained 5 mg of the principal ingredient.

EXAMPLE 7 INJECTION

______________________________________Principal ingredient (Compound of Example 3) 10 gNikkol HCO-60 (a product of Nikko ChemicalCo.) 37Sesame oil 2Sodium chloride 9Propylene glycol 40Phosphate buffer (0.1M, pH 6.0) 100 mlDistilled water ad 1,000 ml______________________________________

The principal ingredient, Nikkol HCO-60, sesame oil and one-half of the propylene glycol were mixed together and heated to about 80.degree. C. to obtain a solution. The solution was added with distilled water heated to about 80.degree. C. in which the phosphate buffer, sodium chloride and the remainder of the propylene glycol had previously been dissolved to obtain 1,000 ml of the solution in total. The aqueous solution was poured in 2 ml ampoules. The ampoules were closed by fusion and then heated for sterilization.

Each ampoule contained 20 mg of the principal ingredient.

Claims

1. A compound having the formula: ##STR10## wherein n is an integer of 8 to 10.

2. A compound according to claim 1 wherein n is 8.

3. A compound according to claim 1 wherein n is 9.

4. A compound according to claim 1 wherein n is 10.

5. A pharmaceutical composition for treating hypertension and hepatic disease comprising a therapeutically effective amount of a compound as claimed in claim 1, in association with a pharmaceutically acceptable carrier, diluent or vehicle.

6. A pharmaceutical composition according to claim 5 wherein n is 9.

7. A method of treating hypertension which comprises administering to a hypertensive subject, a therapeutically effective amount of a pharmaceutical composition as claimed in claim 5.

8. A method of treating hepatic disease which comprises administering to a subject suffering from hepatic disease, a therapeutically effective amount of a pharmaceutical composition as claimed in claim 5.

9. A method which comprises:

(a) reacting a compound having the formula: ##STR11## wherein n is an integer of 8 to 10, with a lower alkyl ester of cyanoacetic acid, in the presence of a base, to form a compound having the formula: ##STR12## wherein n has the same meaning as set forth above and R is a lower alkyl group;

(b) reducing the compound obtained in step (a) with a reducing agent to obtain a compound having the formula: ##STR13## wherein n and R have the same meanings as set forth above: (c) subjecting the compound obtained in step (b) to decarboxylation in the presence of a strong alkali to obtain a compound having the formula: ##STR14## wherein n has the same meaning as set forth above; (d) hydrolyzing the compound obtained in step (c) in the presence of a strong alkali to obtain a compound having the formula: ##STR15## wherein n has the same meaning as set forth above; and (e) reducing the compound obtained in step (d) with a reducing agent to obtain a compound having the formula: ##STR16## wherein n has the same meaning as set forth above.

10. A method as claimed in claim 9 in which said reducing agent is sodium bis(2-methoxyethoxy) aluminum hydride.

11. A method as claimed in claim 9 in which said reducing agent is lithium aluminum hydride.