Patent Application
20170152241
The present invention relates to a novel 5-HT4 receptor agonist, more specifically, a novel bicyclic derivative which comprises pyrimidine ring or pharmaceutically acceptable salt thereof which acts as a 5-HT4 receptor agonist, a method for preparing thereof, a pharmaceutical composition comprising the same, and a use thereof.
Serotonin (5-hydroxytryptamine, 5-HT), one of the neurotransmitters, is broadly distributed throughout human body including both the central nervous system and the peripheral nervous system. Approximately 95% of the human body's total serotonin is found in the gastrointestinal tract, while about 5% thereof is found in the brain. Serotonin receptors are located in intestinal nerves, enterochromaffin cells, intestinal smooth muscle, immune tissues, etc. Serotonin receptor subtypes include 5-HT1, 5-HT2, 5-HT3, 5-HT4, 5-HT6, and 5-HT7. Interactions between these various receptors and serotonin are linked to various physiological functions. Therefore, various researches have been performed for developing therapeutic agents that are capable of interacting with a specific serotonin subtype as a target. The researches include identification of 5-HT4 receptors and active agents interacting therewith (Langlois and Fischmeister, J. Med. Chem. 2003, 46, 319-344).
It has been found by the previous literatures that 5-HT4 receptor agonists are useful for treating an abnormal gastrointestinal motility, i.e., dysfunction in gastrointestinal motility. The abnormal gastrointestinal motility may result in various disorders, for example irritable bowel syndrome (IBS), constipation, dyspepsia, delayed gastric emptying, gastroesophageal reflux disease (GERD), gastroparesis, post-operative ileus, intestinal pseudo-obstruction, drug-induced delayed transit, etc.
Representative 5-HT4 receptor agonists disclosed in prior arts include tegaserod (an aminoguanidine derivative, U.S. Pat. No. 5,510,353), prucalopride (a benzofuran carboxamide derivative, EP0445862), cisapride (a benzamide derivative, U.S. Pat. No. 4,962,115), mosapride (EP 0243959), etc. These compounds are known as an agent stimulating gastrointestinal motility.
The present inventors found that various bicyclic derivatives comprising pyrimidine ring are useful for preventing or treating a dysfunction in gastrointestinal motility by acting as a 5-HT4 receptor agonist.
Therefore, the present invention provides the bicyclic derivative comprising pyrimidine ring or pharmaceutically acceptable salt thereof, a method for preparing thereof, a pharmaceutical composition comprising the same, and a use thereof.
According to one embodiment of the present invention, there is provided a bicyclic derivative comprising pyrimidine ring or pharmaceutically acceptable salt thereof.
According to another embodiment of the present invention, there is provided a method for preparing the bicyclic derivative comprising pyrimidine ring or pharmaceutically acceptable salt thereof.
According to still another embodiment of the present invention, there is provided a pharmaceutical composition for preventing or treating a dysfunction in gastrointestinal motility, which comprise the bicyclic derivative comprising pyrimidine ring or pharmaceutically acceptable salt thereof as an active ingredient.
According to still another embodiment of the present invention, there is provided a use of the bicyclic derivative comprising pyrimidine ring or pharmaceutically acceptable salt thereof for use in the preparation of a medicament for preventing or treating a dysfunction in gastrointestinal motility.
As used herein, the term, “alkyl” refers to a straight or branched hydrocarbon radical. For example, C1-C6 alkyl is an aliphatic hydrocarbon having 1 to 6 carbon atoms, such as methyl, ethyl, propyl, n-butyl, n-pentyl, n-hexyl, isopropyl, isobutyl, sec-butyl, tert-butyl, neopentyl, isopentyl, etc.
In addition, the term “alkoxy or alkyloxy” refers to a radical formed by substituting the hydrogen atom of a hydroxy group with an alkyl group. For example, C1-C6 alkoxy includes methoxy, ethoxy, propoxy, n-butoxy, n-pentyloxy, isopropoxy, sec-butoxy, tert-butoxy, neopentyloxy, isopentyloxy, etc.
Novel Compounds
Compounds of Formula 1 The present invention provides a compound of formula 1 as below, i.e. a bicyclic derivative comprising pyrimidine ring, or a pharmaceutically acceptable salt thereof:
wherein,
R1 is phenyl group; or pyridine group (wherein the phenyl group or pyridine group can be unsubstituted or substituted with one or more of substituents selected from the group consisting of halogen, amino, nitro, cyano, C1-5 alkyl, C1-5 alkyl substituted with halogen, C1-5 alkoxy, C1-5 alkoxy substituted with halogen, and hydroxy),
R2 is each independently hydrogen; halogen; amino; mono- or di-C1-5 alkyl amino; nitro; cyano; C1-5 alkyl; C1-5 alkyl substituted with halogen; C1-5 alkoxy; C1-5 alkoxy substituted with halogen; C1-5 alkoxy carbonyl; hydroxy; or hydroxycarbonyl,
R3 is a substituent selected from the group consisting of the below formulae I to III,
R4 is C1-5 alkyl; C1-5 alkyl substituted with phenyl, thiophene (wherein the phenyl group or thiophene group can be unsubstituted or substituted with one or more of substituents selected from the group consisting of halogen, amino, C1-5 alkyl, C1-5 alkoxy, and hydroxy), or di C1-5 alkyl amino group; or C1-5 alkoxy,
R5 and R5′ are each independently hydrogen; C1-8 alkyl; C1-8 alkyl substituted with phenyl or C3-8 cycloalkyl (wherein the phenyl group can be unsubstituted or substituted with one or more of substituents selected from the group consisting of halogen, amino, C1-5 alkyl, C1-5 alkoxy and hydroxy); or C3-8 cycloalkyl,
ring A is C5-6 cycloalkyl; phenyl; or 5- to 6-membered heteroaryl comprising nitrogen atom,
m is 1 or 2,
n is integer of 0 to 2.
In addition, according to the preferable embodiment of the present invention, in said formulae,
R1 is phenyl group; or pyridine group (wherein the phenyl group or pyridine group can be unsubstituted or substituted with one or more of substituents selected from the group consisting of halogen, amino, nitro, cyano, C1-5 alkyl, C1-5 alkyl substituted with halogen, and C1-5 alkoxy),
R2 is each independently hydrogen; halogen; C1-5 alkyl; C1-5 alkyl substituted with halogen; or C1-5 alkoxy,
R3 is a substituent selected from the group consisting of the below formulae I to III,
R4 is C1-5 alkyl; C1-5 alkyl substituted with phenyl, thiophene, or di C1-5 alkyl amino; or C1-5 alkoxy,
R5 and R5′ are each independently hydrogen; C1-8 alkyl; C1-8 alkyl substituted with phenyl or C3-8 cycloalkyl; or C3-8 cycloalkyl,
ring A is C5-6 cycloalkyl; phenyl; or 5- to 6-membered heteroaryl comprising nitrogen atom,
m is 1 or 2,
n is integer of 0 to 2.
In addition, in said formulae, it is preferable that C1-5 alkyl substituted with halogen of R1 or R2 is trifluoromethyl; it is preferable that C1-5 alkoxy of R2 is methoxy, and it is preferable that C1-5 alkyl of R4 is methyl.
The compound of formula 1 or pharmaceutically acceptable salt thereof may have substituents (for example, substituents of R3) comprising chiral carbon, and in this case the compound of the formula 1 or salt thereof can be present as optical isomers such as (R), (S), racemate (RS), and the like. Therefore, unless otherwise indicated, the compound of formula 1 or pharmaceutically acceptable salt thereof include all of optical isomers such as (R), (S), racemate (RS), and the like.
In addition, the compound of formula 1 or salt thereof can be present geometrical isomers with a double bond cis- or trans-form according to substituents. Therefore, unless otherwise indicated, the compound of formula 1 or salt thereof includes geometrical isomers of cis- and trans-forms.
In addition, the compound of formula 1 or salt thereof can be present as a diastereomer, and unless otherwise indicated, they include all of diastereomers or the mixture thereof.
The compound of formula 1 of the present invention may be a form of the pharmaceutically acceptable salt. The salt may be conventional acid additional salts, for example, salts derived from an inorganic acid such as hydrochloric acid, hydrobromic acid, sulfuric acid, sulfonic acid, sulphamic acid, phosphoric acid or nitric acid and salts derived from organic acid such as acetic acid, propionic acid, succinic acid, glycolic acid, stearic acid, citric acid, maleic acid, malonic acid, methane sulfonic acid, tartaric acid, hydroxymaleic acid, phenylacetic acid, glutamic acid, benzoic acid, salicyclic acid, 2-acetoxybenzoic acid, fumaric acid, toluenesulfonic acid, oxalic acid or trifluoroacetic acid. The said salts can be prepared by reacting the compound of the formula 1 in the form of free base with a stoichiometric amount or excess amount of the desired salt-forming inorganic acid or organic acid in suitable solvents or various mixtures of solvents.
Compounds of Formula 7
The present invention provides a compound of formula 7 as below or a pharmaceutically acceptable salt thereof which can be used as an intermediate for preparing the compound of formula 1.
wherein, R2, R3, A ring, m and n are as defined in the above, and X is halogen.
The compound of formula 7 can be reacted with R1—NH2 to prepare the compound of formula 1.
Methods for Preparing the Novel Compounds
The present invention provides a method for preparing the compound of formula 1 or pharmaceutically acceptable salt thereof, which is bicyclic derivative comprising pyrimidine ring.
Methods for Preparing the Compounds of Formula 1
The method for preparing the compound of formula 1 or pharmaceutically acceptable salt thereof of the present invention may comprise,
performing a halogenation a compound of formula 4 as below to prepare a compound of formula 5 as below;
reacting the compound of formula 5 with a compound of formula 6 as below to prepare a compound of formula 7 as below; and
reacting the compound of formula 7 with R1—NH2 to prepare the compound of formula 1:
wherein, R1, R2, R3, ring A, m and n are as defined in the above, and X is halogen.
The halogenation of the compound of formula 4 may be performed by using a halogenating agent such as phosphorous oxychloride, etc. The halogenation may be preferably performed by stirring at a temperature of between 100° C. and 120° C. overnight. And also, for improving reaction rate and/or yield, the halogenation may be performed in the presence of N,N-dimethylaniline, N,N-dimethylformamide, or diisopropylethylamine, etc. in a catalytic amount.
The reaction between the compound of formula 5 and the compound of formula 6 may be performed under an organic solvent such as tetrahydrofuran, alcohol, chloroform or N,N-dimethylformamide, and the like. The reaction may be performed in a condition of room temperature or elevated temperature (20° C. to 60° C.). And also, for improving reaction rate and/or yield, the reaction may be performed in the presence of a base such as triethylamine or diisopropylethylamine, etc.
The reaction between the compound of formula 7 and R1—NH1 may be performed under an organic solvent such as alcohol, toluene, 1,4-dioxane, N,N-dimethylformamide, etc. or without the solvent. The reaction may be preferably performed by stirring overnight under a condition of elevated temperature (120° C. to 140° C.). And also, for improving reaction rate and/or yield, the reaction may be performed in the presence of a metallic catalyst such as palladium, etc., a ligand and a base such as cesium carbonate, etc., or performed under a microwave (300 W to 600 W).
In addition, the compound of formula 4 may be prepared by reacting a compound of formula 2 as below and a compound of formula 3 as below.
wherein, R2, ring A and n are as defined in the above, and R is hydrogen or C1-5 alkyl.
The cyclization between the compound of formula 2 and the compound of formula 3 may be preferably performed at a temperature of 150° C. to 220° C.
In addition, the compound of formula 4 may be prepared by reacting a compound of formula 8 as below with a compound of formula 9 as below to prepare a compound of formula 10 as below, and then reacting the compound of formula 10 with an acid.
wherein, R2, ring A and n are as defined in the above, and R is hydrogen or C1-5 alkyl.
The reaction between the compound of formula 8 and the compound of formula 9 may be performed in the presence of a base and a solvent. The base may be potassium carbonate, sodium carbonate, etc., and the solvent can be an aqueous solvent such as water, etc. And also, the reaction may be performed at room temperature.
The reaction between the compound of formula 10 and the acid may be performed by using an organic or an inorganic acid, such as acetic acid, hydrochloric acid, etc. The reaction may be preferably performed in an aqueous solvent such as water in a condition of elevated temperature (110° C. to 120° C.).
Methods for Preparing Compounds of Formula 1b
According to one embodiment of the present invention, the present invention provides a method for preparing a compound of formula 1b as below or pharmaceutically acceptable salt thereof, which comprises reacting a compound of formula 1a with an organic acid or an acyl halide.
wherein, R1, R2, R4, ring A, m and n are as defined in the above.
The reaction between the compound of formula 1a and the organic acid may be performed through an amide condensation by using a binding agent such as (benzotriazole-1-yloxy)-tris-(dimethylamino)phosphonium hexafluorophosphate, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride or 1-hydroxybenzotriazole hydrate, etc. and a base such as diisopropylethylamine or triethylamine, etc. The condensation may be performed in an organic solvent such as dichloromethane, N,N-dimethylformamide, N,N-dimethylacetamide, etc. And also, the condensation may be preferably performed at room temperature.
Meanwhile, the reaction between the compound of formula 1a and the acyl halide may be performed through an amide condensation using an organic base such as diisopropylethylamine, triethylamine, etc., or an inorganic base such as sodium hydroxide, etc. The condensation can be performed by using an organic solvent such as dichloromethane, etc. or a mixed solvent of the organic solvent and water. And also, the condensation may be preferably performed at room temperature.
In addition, the compound of formula 1b may be prepared by reacting a compound of formula 7a as below with an organic acid or an acyl halide to prepare a compound of formula 7b, and then reacting the compound of formula 7b with R1—NH2.
wherein, R1, R2, R4, ring A, X, m and n are as defined in the above.
The reaction between the compound of formula 7a and the organic acid may be performed through an amide condensation by using a binding agent such as (benzotriazole-1-yloxy)-tris-(dimethylamino)phosphonium hexafluorophosphate, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride or 1-hydroxybenzotriazole hydrate, etc. and a base such as diisopropylethylamine, triethylamine, etc. The condensation may be performed in an organic solvent such as dichloromethane, N,N-dimethylformamide, N,N-dimethylacetamide, etc. In addition, the condensation may be preferably performed at room temperature.
Meanwhile, the reaction between the compound of formula 7a and the acyl halide may be performed through an amide condensation using an organic base such as diisopropylethylamine, triethylamine, etc. or an inorganic base such as sodium hydroxide, etc. The condensation may be performed by using an organic solvent such as dichloromethane, etc., or a mixed solvent of the organic solvent and water. And also, the condensation may be preferably performed at room temperature.
The reaction between the compound of formula 7b and R1—NH2 may be performed in an organic solvent such as alcohol, toluene, 1,4-dioxane, N,N-dimethylformamide, etc. or without the solvent. The reaction may be preferably performed by stirring overnight in a condition of elevated temperature (120° C. to 140° C.). And also, for improving reaction rate and/or yield, the reaction may be performed in the presence of a metallic catalyst such as palladium, etc., a ligand and a base such as cesium carbonate, etc, or performed under the microwave (300 W to 600 W).
Methods for Preparing Compounds of Formula 1c
According to another embodiment of the present invention, the present invention provides a method for preparing a compound of formula 1c or pharmaceutically acceptable salt thereof, which comprises performing a reductive amination of the compound of formula 1a with an aldehyde or a ketone compound.
wherein, R1, R2, R5, R5′, ring A, m and n are as defined in the above.
The reductive amination may be performed by using a reducing agent such as sodium borohydride, sodium triacetoxy borohydride, sodium cyanoborohydride, etc. The reductive amination may be performed in an organic solvent such as alcohol, etc, and may be performed at room temperature or at a low temperature of 0° C. or below. And also, for improving reaction rate and/or yield, acetic acid, etc. may be added.
Methods for Preparing Compounds of Formula 1d
According to one embodiment of the present invention, a compound of formula 1d may be prepared through introducing an amine-protecting group into a compound of formula 7a to prepare a compound of formula 7c; performing alkylation of the compound of formula 7c to prepare a compound of formula 7d; reacting the compound of formula 7d with R1—NH2, followed by removing the amine-protecting group.
wherein, R1, R2, R5, ring A, X, m and n are as defined in the above, R5′ is hydrogen, and P is an amine-protecting group.
The preferable amine-protecting agent is tert-butoxy carbonyl.
The reaction introducing the amine-protecting group into the compound of formula 7a may be performed in an organic solvent such as dichloromethane, chloroform, 1,4-dioxane, etc. and may be performed at room temperature or at 0° C. or below. And also, triethylamine, diisopropylethylamine, 4-dimethylaminopyridine, etc. may be added.
The alkylation of the compound of formula 7c may be performed by using an alkyl halide. The alkylation may be performed by using a base such as sodium hydride, potassium t-butoxide, etc. in an organic solvent such as N,N-dimethylformamide, etc. The alkylation may be performed at room temperature.
The reaction of the compound of formula 7d with R1—NH2 may be performed in an organic solvent such as alcohol, toluene, 1,4-dioxane, N,N-dimethylformamide, etc. or without the solvent. The reaction may be preferably performed by stirring overnight at the warming temperature condition (120° C. to 140° C.). And also, for improving reaction rate and/or yield, the reaction may be made in the presence of a metallic catalyst such as palladium, etc., a ligand and a base such as cesium carbonate, etc., or performed in the microwave (300 W to 600 W).
In addition, the reaction for removing the amine-protecting group may be performed by using an inorganic acid or an organic acid such as hydrochloric acid, trifluoroacetic acid, etc. in an organic solvent such as ethyl acetate, methanol, etc., and may be preferably performed at room temperature or at 0° C. or below.
In addition, the compound of formula 7d may be prepared through performing an reductive amination a compound of formula 7a as below to prepare a compound of formula 7e; and introducing an amine-protecting group into the compound of formula 7e.
wherein, R2, R5, ring A, X, m and n are as defined in the above, R5′ is hydrogen, and P is an amine protecting group.
The preferable amine-protecting group is tert-butoxycarbonyl.
The reductive amination of the compound of formula 7a may be performed by using a reductive agent such as sodium borohydride, sodium triacetoxy borohydride, sodium cyanoborohydride, etc. The reductive amination may be performed in an organic solvent such as alcohol, etc., and may be performed at room temperature, or at 0° C. or below. And also, for improving reaction rate and yield, acetic acid and the like may be added.
The reaction introducing the amine-protecting group into the compound of formula 7e may be performed in an organic solvent such as dichloromethane, chloroform, 1,4-dioxane, etc. and may be performed at room temperature, or at 0° C. or below. In addition, triethylamine, diisopropylethylamine, 4-dimethylaminopyridine, and the like may be added.
Methods for Preparing Compounds of Formula 1e
According to one embodiment of the present invention, the present invention provides a method for preparing a compound of formula 1e or pharmaceutically acceptable salt thereof, which comprises reacting a compound of formula 7f as below with an organic amine to prepare a compound of formula 7g as below; and reacting the compound of formula 7g with R1—NH2:
wherein, R1, R2, R4, ring A, X, m and n are as defined in the above.
The reaction of the compound of formula 7f with the organic amine may be performed through amide condensation using a binding agent such as (benzotriazole-1-yloxy)-tris-(dimethylamino)phosphonium hexafluorophosphate, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, 1-hydroxybenzotriazole hydrate, etc and a base such as diisopropylethylamine, triethylamine, etc. The condensation may be performed in an organic solvent such as dichloromethane, N,N-dimethylformamide, N,N-dimethylacetamide, etc. And also, the condensation may be preferably performed at room temperature.
The reaction of the compound of formula 7g with R1—NH2 may be performed in an organic solvent such as alcohol, toluene, 1,4-dioxane, N,N-dimethylformamide, etc. or without the solvent. The reaction may be preferably performed by stirring overnight in a condition of elevated temperature (120° C. to 140° C.). And also, for improving reaction rate and/or yield, the reaction may be performed in the presence of a metallic catalyst such as palladium, etc., a ligand and a base such as cesium carbonate, etc., or performed under the microwave (300 W to 600 W).
Methods for Preparing Compounds of Formula 7
According to one embodiment of the present invention, a compound of formula 7 may be prepared by reacting a compound of formula 5 as below with a compound of formula 6 to prepare a compound of formula 7:
wherein, R2, R3, ring A, m and n are as defined in the above, and X is halogen.
The reaction between the compound of formula 5 and the compound of formula 6 may be performed in an organic solvent such as tetrahydrofuran, alcohol, chloroform or N,N-dimethylformamide, etc. In addition, the reaction may be performed at room temperature or elevated temperature (20° C. to 60° C.). And also, for improving the reaction rate and/or yield, the reaction may be performed in the presence of a base such as triethylamine or diisopropylethylamine, etc.
Pharmaceutical Compositions Comprising the Novel Compounds
The present invention provides a pharmaceutical composition for preventing or treating a dysfunction in gastrointestinal motility, which comprise a therapeutically effective amount of the compound of formula 1 or pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier.
The dysfunction in gastrointestinal motility includes, for example, gastrointestinal diseases such as gastroesophageal reflux disease (GERD), constipation, irritable bowel syndrome (IBS), dyspepsia, post-operative ileus, delayed gastric emptying, gastroparesis, intestinal pseudo-obstruction, drug-induced delayed transit, diabetic gastric atony and the like. The constipation includes chronic constipation, chronic idiopathic constipation (CIO), opioid-induced constipation (OIC), etc. And also, the dyspepsia includes non-ulcerative dyspepsia and functional dyspepsia.
The pharmaceutical composition may comprises pharmaceutically acceptable carriers such as diluents, disintegrants, sweeteners, lubricants, flavoring agents, etc. as commonly used. The pharmaceutical composition and may be prepared as an oral dosage form such as tablets, capsules, powders, granules and suspensions, emulsions or syrups; or a parenteral dosage form such as injection, according to the conventional methods. The dosage form may be prepared into the various forms, for example, dosage forms for single administration or dosage forms for multiple administrations.
The pharmaceutical composition of the present invention may comprise a diluent such as lactose, corn starch, etc, a lubricant such as magnesium stearate, etc., an emulsifying agent, a suspending agent, a stabilizer, an isotonic agent, etc. If necessary, the composition further comprises a sweetener and/or a flavoring agent.
The pharmaceutical composition of the present invention may be administered orally or parenterally including intravenous, intraperitoneal, subcutaneous, rectal and topical routes of administration. Therefore, the composition of the present invention may be prepared into various dosage forms such as tablets, capsules, aqueous solutions or suspensions, etc. In the case of tablets for oral administration, a carrier such as lactose, corn starch, etc., and a lubricant such as magnesium stearate are commonly used. In the case of the capsules for oral administration, lactose and/or dried corn starch can be as a diluent. When an aqueous suspension is required for oral administration, an active ingredient may be combined with an emulsifying agent and/or a suspending agent. If necessary, certain sweetening agent and/or flavoring agent may be added. For intramuscular, intraperitoneal, subcutaneous and intravenous administrations, sterile solutions of the active ingredient are usually prepared, and the pH of the solutions should be suitably adjusted and buffered. For intravenous administration, the total concentration of solutes should be controlled in order to render the preparation isotonic. The composition of the present invention may be in the form of an aqueous solution comprising a pharmaceutically acceptable carrier such as brine of pH 7.4. The solutions may be introduced into a patient's intramuscular blood-stream by local bolus injection.
The compound of formula 1 or pharmaceutically acceptable salt thereof may be administered in a therapeutically effective amount ranging from about 0.001 mg/kg to about 10 mg/kg per day to a subject patient. Of course, the dosage may be changed according to age, weight, susceptibility, and symptom of the patient or activity of the compound.
In addition, the present invention provides a use of the compound of formula 1 or pharmaceutically acceptable salt thereof for preparing a medicament for the prevention or treatment of dysfunction in gastrointestinal motility, for example, gastroesophageal reflux disease (GERD), constipation, irritable bowel syndrome (IBS), dyspepsia, post-operative ileus, delayed gastric emptying, gastroparesis, intestinal pseudo-obstruction, drug-induced delayed transit, or diabetic gastric atony.
Method for Preventing or Treating Gastrointestinal Dysmotility
In addition, the present invention provides a method for preventing or treating dysfunction in gastrointestinal motility, for example, gastroesophageal reflux disease (GERD), constipation, irritable bowel syndrome (IBS), dyspepsia, post-operative ileus, delayed gastric emptying, gastroparesis, intestinal pseudo-obstruction, drug-induced delayed transit, or diabetic gastric atony, which comprises administering the composition comprising the compound of formula 1 or pharmaceutically acceptable salt thereof as an active ingredient to a subject in need of it.
The composition used in the prevention or treatment of the present invention includes the pharmaceutical composition disclosed in the present specification.
In addition, the subject needed the prevention or treatment method includes a mammal, in particular a human.
The compound according to the present invention, i.e., the bicyclic derivative comprising pyrimidine ring or pharmaceutically acceptable salt thereof act as a 5-HT4 receptor agonist, and thus can be usefully applied for the prevention or treatment of gastrointestinal diseases such as dysfunction in gastrointestinal motility, for example, gastroesophageal reflux disease (GERD), constipation, irritable bowel syndrome (IBS), dyspepsia, post-operative ileus, delayed gastric emptying, gastroparesis, intestinal pseudo-obstruction, drug-induced delayed transit, diabetic gastric atony, and the like.
Hereinafter, the present invention will be explained more specifically via reference examples, examples and experimental examples. However, such reference examples, examples and experimental examples merely exemplify the present invention, and are not intended to limit the present invention to them.
Nuclear Magnetic Resonance (NMR) spectrum analysis of the compounds prepared in the reference examples and examples was performed on Bruker 400 MHz spectrometer, a chemical shift was analyzed in ppm, a column chromatography was performed on silica gel (Merck, 70-230 mesh) (W. C. Still, J. Org. Chem., 43, 2923, 1978). And also, the starting materials in each example were synthesized from the known compounds according to the references, or were obtained from Sigma Aldrich.
(3S)-(−)-3-(tert-butoxycarbonylamino)pyrrolidine (1.08 g, 5.79 mmol) was added into a mixed solution of 2,4-dichloro-6,7-dimethoxyquinazoline (1 g, 3.86 mmol) in ethanol (15 mL), and then the reaction mixture was stirred at room temperature overnight. The solvent was concentrated under reduced pressure, and the resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1) to give the titled compound (970 mg) as a pale yellow solid.
1H-NMR (400 MHz, CD3OD) δ 7.37 (s, 1H), 7.14 (s, 1H), 4.76 (m, 1H), 4.38 (m, 1H), 4.19 (m, 1H), 4.07 (m, 1H), 3.97 (s, 3H), 3.96 (s, 3H), 3.80 (m, 1H), 2.29 (m, 1H), 2.05 (m, 1H), 1.45 (s, 9H).
(3S)-(−)-3-(methylamino)pyrrolidine (580 mg, 5.79 mmol) was added into a mixed solution of 2,4-dichloro-6,7-dimethoxyquinazoline (1 g, 3.86 mmol) in ethanol (15 mL), and then the reaction mixture was stirred at room temperature overnight. The solvent was concentrated under reduced pressure, and the resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1) to give the titled compound (950 mg) as a pale yellow solid.
1H-NMR (400 MHz, CD3OD) δ 7.48 (s, 1H), 6.93 (s, 1H), 4.11 (m, 2H), 3.99 (m, 1H), 3.93 (s, 3H), 3.92 (s, 3H), 3.75 (m, 1H), 3.40 (m, 1H), 2.46 (s, 3H), 2.28 (m, 1H), 1.99 (m, 1H).
(3S)-(−)-3-(ethylamino)pyrrolidine (580 mg, 5.79 mmol) was added into a mixed solution of 2,4-dichloro-6,7-dimethoxyquinazoline (1 g, 3.86 mmol) in ethanol (15 mL), and then the reaction mixture was stirred at room temperature overnight. The solvent was concentrated under reduced pressure, and the resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1) to give the titled compound (980 mg) as a pale yellow solid.
1H-NMR (400 MHz, CD3OD) δ 7.48 (s, 1H), 6.92 (s, 1H), 4.13 (m, 2H), 3.93 (m, 1H), 3.93 (s, 3H), 3.92 (s, 3H), 3.70 (m, 1H), 3.48 (m, 1H), 2.76 (m, 2H), 2.30 (m, 1H), 1.95 (m 1H), 1.17 (t, 3H).
(3S)-(−)-3-acetamidopyrrolidine (742 mg, 5.79 mmol) was added into a mixed solution of 2,4-dichloro-6,7-dimethoxyquinazoline (1 g, 3.86 mmol) in ethanol (15 mL), and then the reaction mixture was stirred at room temperature overnight. The solvent was concentrated under reduced pressure, and the resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1) to give the titled compound (940 mg) as a pale yellow solid.
1H-NMR (400 MHz, DMSO-d6) δ 8.17 (d, 1H), 7.50 (s, 1H), 7.11 (s, 1H), 4.35 (m, 1H), 4.11 (m, 1H), 4.07 (m, 2H), 3.98 (s, 3H), 3.90 (s, 3H), 3.70 (m, 1H), 2.18 (m, 1H), 2.11 (m, 1H), 1.81 (s, 3H).
(R)-3-aminopiperidine dihydrochloride (1 g, 5.79 mmol) and N,N-dimethylisopropylamine (2.7 mL, 15.44 mmol) were added into a mixed solution of 2,4-dichloro-6,7-dimethoxyquinazoline (1 g, 3.86 mmol) in ethanol (15 mL), and then the reaction mixture was stirred at room temperature overnight. The solvent was concentrated under reduced pressure, and the resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=20/1) to give the titled compound (1.1 g) as a pale yellow solid.
1H-NMR (400 MHz, DMSO) δ 7.22 (s, 1H), 7.12 (s, 1H), 4.21 (m, 1H), 4.00 (m, 1H), 3.98 (s, 6H), 3.62 (m, 3H), 2.21 (m, 1H), 1.98 (m, 1H), 1.82 (m, 2H).
Acetyl chloride (121 μL, 1.70 mmol) and triethylamine (544 μL, 3.86 mmol) were added into a mixed solution of (R)-1-(2-chloro-6,7-dimethoxyquinazolin-4-yl)piperidin-3-amine (500 mg, 1.55 mmol) prepared in Step 1 and dichloromethane (10 ml), and then they were stirred at room temperature overnight. The solvent was concentrated under reduced pressure, and the resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1) to give the titled compound (450 mg) as a pale yellow solid.
Urea (5.4 g, 89.7 mmol) was added to 2-amino-5-methoxybenzoic acid (5 g, 29.9 mmol), and then the reaction mixture was stirred at 200° C. for 1 hour, cooled to room temperature and stirred for 1 hour. Water (30 ml) was added into the reaction mixture, and the reaction mixture was stirred at room temperature for 1 hour. The resulting precipitate was filtered and dried in vacuo to give the titled compound (4.8 g) as a pale yellow solid.
N,N-dimethylamine (6.3 mL, 50 mmol) was added into a mixed solution of 6-methoxyquinazolin-2,4(1H,3H)-dione (4.8 g, 25.0 mmol) prepared in Step 1 in phosphorus oxychloride (50 mL), and then they were stirred at 110° C. overnight. After cooling to room temperature, the reaction mixture was added into ice water and then basified to pH 9 with sodium hydroxide. The aqueous layer was extracted with ethyl acetate, and the organic layer was dried on anhydrous sodium sulfate and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=5/1) to give the titled compound (3.6 g) as a yellow solid.
(3S)-(−)-3-acetamidopyrrolidine (419 mg, 3.27 mmol) was added into a reaction solution of 2,4-dichloro-6-methoxyquinazoline (500 mg, 2.18 mmol) prepared in Step 2 in ethanol (10 mL), and then the reaction mixture was stirred at room temperature overnight. The solvent was concentrated under reduced pressure, and the resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1) to give the titled compound (360 mg) as a yellow solid.
(3S)-(−)-3-(methylamino)pyrrolidine (327 mg, 3.27 mmol) was added into a mixed solution of 2,4-dichloro-6-methoxyquinazoline (500 mg, 2.18 mmol) prepared in Step 2 of Reference Example 6 in ethanol (10 mL), and then the reaction mixture was stirred at room temperature overnight. The solvent was concentrated under reduced pressure, and the resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1) to give the titled compound (400 mg) as a pale yellow solid.
(3S)-(−)-3-(ethylamino)pyrrolidine (373 mg, 3.27 mmol) was added into a mixed solution of 2,4-dichloro-6-methoxyquinazoline (500 mg, 2.18 mmol) prepared in Step 2 of Reference Example 6 in ethanol (10 mL), and then the reaction mixture was stirred at room temperature overnight. The solvent was concentrated under reduced pressure, and the resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1) to give the titled compound (420 mg) as a pale yellow solid.
(R)-3-aminopiperidine dihydrochloride (567 mg, 3.27 mmol) and N,N-dimethylisopropylamine (570 μL, 3.27 mmol) were added into a mixed solution of 2,4-dichloro-6-methoxyquinazoline (500 mg, 2.18 mmol) prepared in Step 2 of Reference Example 6 in ethanol (10 mL), and then the reaction mixture was stirred at room temperature overnight.
The solvent was concentrated under reduced pressure, and the resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=20/1) to give the titled compound (420 mg) as a pale yellow solid.
Acetyl chloride (101 μL, 1.43 mmol) and triethylamine (602 μL, 4.29 mmol) were added into a mixed solution of (R)-1-(2-chloro-6-methoxyquinazolin-4-yl)piperidin-3-amine (420 mg, 1.43 mmol) prepared in Step 1 in dichloromethane (10 mL), and then they were stirred at room temperature overnight. The solvent was concentrated under reduced pressure, and the resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1) to give the titled compound (400 mg) as a pale yellow solid.
Urea (4.4 g, 73.1 mmol) was added to 2-amino-4-(trifluoromethyl)benzoic acid (5 g, 24.4 mmol), and then the reaction mixture was stirred at 200° C. for 1 hour. After cooling to room temperature, the reaction mixture was stirred for 1 hour. Water (100 ml) was added thereto, and the reaction mixture was stirred at room temperature for 1 hour. The resulting precipitate was filtered and dried in vacuo to give the titled compound (5 g) as a pale green solid.
1H-NMR (400 MHz, DMSO-d6) δ 11.58 (s, 1H), 11.41 (s, 1H), 8.08 (d, 1H), 7.48 (d, 1H), 7.45 (s, 1H).
7-(trifluoromethyl)quinazolin-2,4(1H, 3H)-dione (5 g, 21.7 mmol) prepared in Step 1 was added into phosphorus oxychloride (30 mL), and then they were stirred at 110° C. overnight. After cooling to room temperature, the reaction mixture was added into ice water and then basified to pH 9 with sodium hydroxide. The aqueous layer was extracted with ethyl acetate, and the organic layer was dried on anhydrous sodium sulfate and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=5/1) to give the titled compound (4.5 g) as a yellow solid.
1H-NMR (400 MHz, CDCl3) δ 8.42 (d, 1H), 8.32 (s, 1H), 7.92 (d, 1H).
(S)-(−)-3-(methylamino)pyrrolidine (563 mg, 5.62 mmol) was added into a mixed solution of 2,4-dichloro-7-(trifluoromethyl)quinazoline (1 g, 3.74 mmol) prepared in Step 2 in ethanol (15 mL), and then the reaction mixture was stirred at room temperature overnight. The solvent was concentrated under reduced pressure, and the resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1) to give the titled compound (560 mg) as a yellow solid.
1H-NMR (400 MHz, CDCl3) δ 8.51 (d, 1H), 7.89 (s, 1H), 7.69 (d, 1H), 4.17 (m, 3H), 3.90 (m, 1H), 3.52 (m, 1H), 2.51 (s, 3H), 2.35 (m, 1H), 2.09 (m, 1H).
(S)-(−)-3-(ethylamino)pyrrolidine (642 mg, 5.62 mmol) was added into a mixed solution of 2,4-dichloro-7-(trifluoromethyl)quinazoline (1 g, 3.74 mmol) in ethanol (15 mL), and then the reaction mixture was stirred at room temperature overnight. The solvent was concentrated under reduced pressure, and the resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1) to give the titled compound (680 mg) as a yellow solid.
1H-NMR (400 MHz, CDCl3) δ 8.50 (m, 1H), 7.88 (m, 1H), 7.69 (m, 1H), 4.16 (m, 3H), 3.83 (m, 1H), 3.59 (m, 1H), 2.32 (m, 2H), 2.32 (m, 1H), 2.04 (m, 1H), 1.19 (t, 3H).
(S)-(−)-3-acetamidopyrrolidine (720 mg, 5.62 mmol) was added into a mixed solution of 2,4-dichloro-7-(trifluoromethyl)quinazoline (1 g, 3.74 mmol) in ethanol (15 mL), and then the reaction mixture was stirred at room temperature overnight. The solvent was concentrated under reduced pressure, and the resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1) to give the titled compound (600 mg) as a yellow solid.
1H-NMR (400 MHz, CDCl3) δ 8.48 (m, 1H), 7.88 (m, 1H), 7.69 (m, 1H), 4.51 (m, 1H), 4.22-4.09 (m, 3H), 3.88 (m, 1H), 2.30 (m, 1H), 2.11 (m, 1H), 1.95 (s, 3H).
A mixture of 2-aminobenzoic acid (3 g, 21.5 mmol) and urea (3.9 g, 64.5 mmol) was stirred at 200° C. for 2 hours. After cooling the reaction solution, water was added thereto and the reaction solution was stirred for 1 hour. The resulting yellow solid was filtered, washed with water and dried in vacuo to give the titled compound (2.5 g). This compound was used in the subsequent reaction without further purification.
A mixture of quinazolin-2,4-diol (2.3 g, 14.2 mmol) prepared in Step 1 and phosphorus oxychloride (10 ml) was stirred at reflux overnight. After cooling the reaction mixture to room temperature, the reaction mixture was added into ice water and then basified to pH 7-8 with sodium hydroxide. The resulting yellow precipitate was filtered, washed with water and dried in vacuo to give the titled compound (2.5 g).
1H NMR (400 MHz, CDCl3) δ 8.28 (d, 1H), 8.05-8.00 (m, 2H), 7.80-7.70 (m, 1H).
3-((S)-tert-butoxycarbonylamino)pyrrolidine (3.37 g, 18.1 mmol) was added into ethanol/chloroform (40/40 ml) solution of 2,4-dichloroquinazoline (3 g, 15.1 mmol) prepared in Step 2 and diisopropylethylamine (3.15 ml, 18.1 mmol), and then they were stirred at room temperature for 1 hour. The reaction mixture was concentrated, diluted in chloroform, and then washed with water, dried with anhydrous sodium sulfate and concentrated. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1) to give the titled compound (3.51 g) as a white solid.
1H NMR (400 MHz, CDCl3) δ 8.09 (d, 1H), 7.77 (d, 1H), 7.71 (t, 1H), 7.40 (t, 1H), 4.70 (m, 1H), 4.39 (m, 1H), 4.22 (m, 1H), 4.10-4.02 (m, 2H), 3.86 (m, 1H), 2.30 (m, 1H), 2.04 (m, 1H), 1.45 (s, 9H).
Sodium hydride (15.5 mg, 0.39 mmol, 60 wt %) was added into N,N-dimethylformamide (1.5 ml) solution of (S)-tert-butyl 1-(2-chloroquinazolin-4-yl)pyrrolidin-3-ylcarbamate (90 mg, 0.26 mmol) prepared in Reference Example 13 at 0° C., and they were stirred for 30 minutes. 1-Bromopropane (28 μl, 0.31 mmol) was added into the reaction solution, and then they were stirred at room temperature overnight. Water was added to terminate the reaction, and extracted with ethyl acetate. The extract was dried with anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=2/1) to give the titled compound (57.7 mg) as a colorless oil.
1H NMR (400 MHz, CDCl3) δ 8.09 (d, 1H), 7.56 (d, 1H), 7.70 (t, 1H), 7.39 (t, 1H), 4.60 (m, 1H), 4.12 (m, 2H), 3.93 (m, 2H), 3.21 (m, 1H), 3.10 (m, 1H), 2.22 (m, 2H), 1.70 (m, 2H), 1.48 (s, 9H), 0.91 (t, 3H).
The titled compound was prepared as a colorless oil in the same manner as Reference Example 14 by using (S)-tert-butyl 1-(2-chloroquinazolin-4-yl)pyrrolidin-3-ylcarbamate prepared in Reference Example 13 and 1-bromobutane.
1H NMR (400 MHz, CDCl3) δ 8.09 (d, 1H), 7.75 (d, 1H), 7.69 (t, 1H), 7.39 (t, 1H), 4.59 (m, 1H), 4.09 (m, 2H), 3.91 (m, 2H), 3.25 (m, 1H), 3.11 (m, 1H), 2.22 (m, 2H), 1.57 (m, 2H), 1.48 (s, 9H), 1.30 (m, 2H), 0.92 (t, 3H); (Yield: 50%).
The titled compound was prepared as a colorless oil in the same manner as Reference Example 14 by using (S)-tert-butyl 1-(2-chloroquinazolin-4-yl)pyrrolidin-3-ylcarbamate prepared in Reference Example 13 and 1-bromopentane.
1H NMR (400 MHz, CDCl3) δ 8.08 (d, 1H), 7.74 (d, 1H), 7.67 (m, 1H), 7.39 (t, 1H), 4.60 (m, 1H), 4.09 (t, 2H), 3.97-3.86 (m, 2H), 3.23 (m, 1H), 3.10 (m, 1H), 2.22 (m, 2H), 1.59 (m, 1H), 1.48 (m, 9H+1H), 1.32 (m, 4H), 0.91 (t, 3H); (Yield: 56%).
The titled compound was prepared as a colorless oil in the same manner as Reference Example 14 by using (S)-tert-butyl 1-(2-chloroquinazolin-4-yl)pyrrolidin-3-ylcarbamate prepared in Reference Example 13 and 1-iodohexane.
1H NMR (400 MHz, CDCl3) δ 8.09 (d, 1H), 7.74 (d, 1H), 7.67 (t, 1H), 7.38 (t, 1H), 4.60 (m, 1H), 4.11 (m, 2H), 3.90 (m, 2H), 3.23 (m, 1H), 3.11 (m, 1H), 2.22 (m, 2H), 1.59 (m, 2H), 1.48 (s, 9H), 1.30 (m, 6H), 0.89 (t, 3H); (Yield: 66%).
A mixture of 2-amino-3-methoxybenzoic acid (5 g, 29.9 mmol) and urea (8.98 g, 149.5 mmol) was stirred at 220° C. for 4 hours. After cooling the reaction solution, water was added thereto and the reaction solution was stirred for 1 hour. The resulting yellow solid was filtered, washed with water and dried in vacuo to give the titled compound (5.5 g). This compound was used in the subsequent reaction without further purification.
A mixture of 8-methoxyquinazoline-2,4-diol (5.5 g, 28.6 mmol) prepared in Step 1 and phosphorus oxychloride (25 ml) was stirred at reflux overnight. After cooling the reaction mixture to room temperature, the reaction mixture was added into ice water and then basified to pH 7-8 with sodium hydroxide. The aqueous layer was extracted with dichloromethane, and the organic layer was dried on anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=5/1) to give the titled compound (2.1 g) as a white solid.
1H NMR (400 MHz, CDCl3) δ 7.83 (d, 1H), 7.65 (t, 1H), 7.34 (d, 1H), 4.09 (s, 3H).
Diisopropylethylamine (0.23 ml, 1.31 mmol) was added into ethanol/chloroform (10/10 ml) solution of 2,4-dichloro-8-methoxyquinazoline (300 mg, 1.31 mmol) prepared in Reference Example 18 and (S)-3-acetamidopyrrolidine (201 mg, 1.57 mmol), and then they were stirred at room temperature overnight. The reaction solution was concentrated under reduced pressure, diluted with dichloromethane, washed with water, dried with anhydrous magnesium sulfate, and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=20/1) to give the titled compound (357.5 mg) as a yellow oil.
1H NMR (400 MHz, CDCl3) δ 7.65 (d, 1H), 7.06 (d, 1H), 6.65 (s, 1H), 4.68 (brs, 1H), 4.14-3.91 (m, 3H+5H), 2.30 (m, 1H), 2.17 (m, 1H), 1.98 (s, 3H).
The titled compound was prepared as a yellow oil in the same manner as Reference Example 19 by using 2,4-dichloro-8-methoxyquinazoline prepared in Reference Example 18 and (3S)-(−)-3-(ethylamino)pyrrolidine.
1H NMR (400 MHz, CDCl3) δ 7.69 (m, 1H), 7.29-7.23 (m, 1H), 7.09 (m, 1H), 4.13 (m, 2H), 3.97 (m, 3H+1H), 3.74 (m, 1H), 3.50 (m, 1H), 2.73 (m, 2H), 2.20 (m, 1H), 1.92 (m, 1H), 1.13 (m, 3H); (Yield: 98%).
(R)-(−)-3-aminopiperidine dihydrochloride (249 mg, 1.44 mmol) was added into ethanol/chloroform (10/10 ml) solution of 2,4-dichloro-8-methoxyquinazoline (300 mg, 1.31 mmol) prepared in Reference Example 18 and diisopropylethylamine (0.68 ml, 4.23 mmol), and then they were stirred at room temperature overnight. The reaction solution was concentrated under reduced pressure, diluted with dichloromethane, washed with water, dried with anhydrous magnesium sulfate, and concentrated under reduced pressure. The resulting residue was dissolved in dichloromethane (10 ml). Triethylamine (0.33 ml, 2.39 mmol) and acetyl chloride (0.13 ml, 1.75 mmol) were added thereto at 0° C., and they were stirred at room temperature overnight. The reaction solution was concentrated under reduced pressure, diluted with dichloromethane, washed with water, dried with anhydrous magnesium sulfate, and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=100/1) to give the titled compound (400 mg) as a white solid.
1H NMR (400 MHz, CDCl3) δ 7.51 (d, 1H), 7.41 (m, 1H), 7.11 (d, 1H), 6.73 (s, 1H), 4.17 (m, 1H), 4.03 (s, 3H), 3.87-3.79 (m, 4H), 2.02 (s, 3H), 1.93 (m, 2H), 1.74 (m, 1H), 1.71 (m, 1H).
A mixture of 2-amino-6-methylbenzoic acid (5 g, 33.1 mmol) and urea (9.93 g, 165 mmol) was stirred at 150° C. for 6 hours. Water was added thereto at 100° C. and they were stirred at room temperature overnight. After cooling the reaction mixture to room temperature, the filtered solid was dissolved in 0.2 N sodium hydroxide aqueous solution (100 ml). The reaction mixture was stirred at reflux for 4 hours and stirred at room temperature for 1 day. Conc. hydrochloric acid aqueous solution was added thereto to neutralize to pH 7 and the resulting solid was filtered and dried in vacuo to give the titled compound (3 g) as a white solid.
A mixture of the prepared white solid (3 g, 17.0 mmol), N,N-dimethylaniline (4.3 ml, 34.1 mmol) and phosphorus oxychloride (12 ml) was stirred at reflux for 4 hours. After cooling the reaction mixture to room temperature, the same was added into ice water and the aqueous layer was extracted with dichloromethane. The organic layer was dried on anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=50/1) to give the titled compound (2 g) as a yellow solid.
1H NMR (400 MHz, CDCl3) δ 7.88 (d, 1H), 7.83 (m, 1H), 7.50 (d, 1H), 3.03 (s, 3H).
The titled compound was prepared as a white solid in the same manner as Reference Example 13 by using 2,4-dichloro-5-methylquinazoline prepared in Reference Example 22 and (S)-3-acetamidopyrrolidine.
1H NMR (400 MHz, CDCl3) δ 7.53 (m, 1H), 7.43 (d, 1H), 7.26 (m, 1H), 6.03 (m, 1H), 4.51 (m, 1H), 3.95 (m, 2H), 3.78 (m, 1H), 3.59 (m, 1H), 2.63 (s, 3H), 2.29 (m, 1H), 1.92 (m, 4H); (Yield: 59%).
The titled compound was prepared as a pale yellow oil in the same manner as Reference Example 13 by using 2,4-dichloro-5-methylquinazoline prepared in Reference Example 22 and (3S)-(−)-3-(ethylamino)pyrrolidine.
1H NMR (400 MHz, CDCl3) δ 7.54 (m, 2H), 7.21 (m, 1H), 3.84-3.74 (m, 3H), 3.50 (m, 1H), 3.36 (m, 1H), 2.64 (m, 2H+3H), 2.09 (m, 1H), 1.78 (m, 1H), 1.10 (t, 3H); (Yield: 66%).
The titled compound was prepared as a pale yellow oil in the same manner as Reference Example 21 by using 2,4-dichloro-5-methylquinazoline prepared in Reference Example 22.
1H NMR (400 MHz, CDCl3) δ 7.64 (m, 2H), 7.27 (m, 1H), 4.11 (m, 1H), 3.90 (m, 1H), 3.57 (m, 2H), 3.43 (m, 1H), 2.70 (s, 3H), 2.00 (s, 3H), 1.78 (m, 2H), 1.60 (m, 2H); (Yield: 56%).
A mixture of 2-amino-3-methylbenzoic acid (5 g, 33.1 mmol) and urea (5.96 g, 99.2 mmol) was stirred at 190° C. for 4 hours. After cooling the reaction solution to room temperature, water (70 ml) was added thereto and the reaction solution was stirred for 1 hour. The resulting solid was filtered and dried in vacuo to give the titled compound (4.88 g) as a white solid.
1H NMR (400 MHz, DMSO-d6) δ 11.29 (brs, NH), 10.42 (brs, NH), 7.77 (d, 1H), 7.48 (d, 1H), 7.10 (t, 1H), 2.35 (s, 3H).
A mixture of 8-methylquinazoline-2,4(1H,3H)-dione (4.88 g, 27.7 mmol) prepared in Step 1, N,N-dimethylaniline (2.8 ml, 22.2 mmol) and phosphorus oxychloride (28 ml) was stirred at reflux for 4 hours. After cooling the reaction mixture to room temperature, the reaction mixture was added into ice water. The resulting solid was filtered, washed with water, and dried in vacuo to give the titled compound (5.28 g) as a white solid.
1H NMR (400 MHz, CDCl3) δ 8.12 (d, 1H), 7.83 (d, 1H), 7.62 (t, 1H), 2.75 (s, 3H).
The titled compound was prepared as a white solid in the same manner as Reference Example 13 by using 2,4-dichloro-8-methylquinazoline prepared in Reference Example 26 and (S)-3-acetamidopyrrolidine.
1H NMR (400 MHz, CD3OD) δ 7.87 (d, 1H), 7.50 (d, 1H), 7.22 (t, 1H), 7.10 (m, 1H), 4.57 (m, 1H), 4.03-3.80 (m, 4H), 2.56 (s, 3H), 2.20 (m, 1H), 2.10 (m, 1H), 2.03 (s, 3H); (Yield: 73%).
The titled compound was prepared as a white solid in the same manner as Reference Example 13 by using 2,4-dichloro-8-methylquinazoline prepared in Reference Example 26 and (3S)-(−)-3-(ethylamino)pyrrolidine.
1H NMR (400 MHz, CDCl3) δ 7.93 (d, 1H), 7.51 (d, 1H), 7.24 (t, 1H), 4.11 (m, 2H), 3.95 (m, 1H), 3.75 (m, 1H), 3.52 (m, 1H), 2.73 (m, 2H), 2.62 (s, 3H), 2.28-2.18 (m, 2H), 1.15 (m, 3H); (Yield: 78%).
The titled compound was prepared as a white solid in the same manner as Reference Example 21 by using 2,4-dichloro-8-methylquinazoline prepared in Reference Example 26.
1H NMR (400 MHz, CDCl3) δ 7.79 (d, 1H), 7.58 (d, 1H), 7.36 (t, 1H), 7.07 (brs, NH), 4.15 (m, 1H), 3.86-3.75 (m, 4H), 2.65 (s, 3H), 2.03 (s, 3H), 1.92 (m, 2H), 1.78-1.71 (m, 2H); (Yield: 61%).
The titled compound was prepared as a white solid in the same manner as Step 1 of Reference Example 18 by using methyl 2-amino-4-chlorobenzoate.
1H NMR (400 MHz, DMSO-d6) δ 11.34 (brs, 2NH), 7.87 (d, 1H), 7.18 (m, 2H); (Yield: 98%).
Diisopropylethylamine (9.21 ml, 52.9 mmol) was added to a mixture of 7-chloroquinazoline-2,4(1H,3H)-dione (5.2 g, 26.5 mmol) prepared in Step 1 and phosphorus oxychloride (26 ml), and they were stirred at reflux for 4 hours. After cooling the reaction mixture to room temperature, the same was added into ice water, and basified to pH 7-8 by using sodium bicarbonate. The aqueous layer was extracted with dichloromethane, and the organic layer was dried on anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane) to give the titled compound (3.88 g) as a white solid.
1H NMR (400 MHz, CDCl3) δ 8.22 (d, 1H), 8.01 (s, 1H), 7.68 (d, 1H).
The titled compound was prepared as a white solid in the same manner as Reference Example 13 by using 2,4,7-trichloroquinazoline prepared in Reference Example 30 and (3S)-(−)-3-(methylamino)pyrrolidine.
1H NMR (400 MHz, CD3OD) δ 8.32 (d, 1H), 7.67 (s, 1H), 7.51 (d, 1H), 4.30-4.15 (m, 4H), 4.00 (m, 1H), 2.83 (s, 3H), 2.58 (m, 1H), 2.37 (m, 1H); (Yield: 88%).
A mixture of 4-fluoroanthranilic acid (5 g, 32.2 mmol) and urea (5.8 g, 96.7 mmol) was stirred at 220° C. for 1 hour. After cooling the reaction solution, water was added thereto. The reaction solution was stirred at reflux for 1 hour, and stirred again at room temperature for 3 days. The resulting solid was filtered, washed with water and dried in vacuo to give the titled compound (5.26 g) as a pale yellow solid.
1H NMR (400 MHz, DMSO-d6) δ 11.29 (brs, 2NH), 7.95 (t, 1H), 7.03 (t, 1H), 6.91 (d, 1H).
A mixture of 7-fluoroquinazoline-2,4(1H, 3H)-dione (5.26 g, 29.2 mmol) prepared in Step 1 and phosphorus oxychloride (85 ml) was stirred at reflux for 3 days. After cooling the reaction mixture to room temperature, the same was added into ice water. The resulting solid was filtered and dried in vacuo to give the titled compound (3.82 g) as a yellow solid.
1H NMR (400 MHz, CDCl3) δ 8.32 (m, 1H), 7.63 (d, 1H), 7.49 (t, 1H).
The titled compound was prepared as a white solid in the same manner as Reference Example 19 by using 2,4,-dichloro-7-fluoroquinazoline prepared in Step 2.
1H NMR (400 MHz, CD3OD) δ 8.35 (m, 1H), 7.27 (m, 2H), 4.50 (m, 1H), 4.19-4.04 (m, 3H), 3.83 (m, 1H), 2.31 (m, 1H), 2.11 (m, 1H), 1.95 (s, 3H); (Yield: 33%).
An aqueous solution (110 ml) of ethyl 2-cyclohexanone carboxylate (10 ml, 62.9 mmol), 2-methyl-2-thiopseudourea (9.6 g, 69.1 mmol) and sodium carbonate (10.7 g, 101 mmol) was stirred at room temperature for 4 days. The resulting solid was filtered, dried in vacuo and used in the subsequent reaction without further purification.
A mixture of 2-(methylthio)-5,6,7,8-tetrahydro-quinazolin-4-ol prepared in Step 1, acetic acid (65 ml) and water (30 ml) was stirred at reflux for 3 days. After cooling the reaction solution to room temperature, the resulting solid was filtered, washed with ethyl acetate and dried in vacuo to give the titled compound (4.85 g) as a white solid
1H NMR (400 MHz, DMSO-d6) δ 10.84 (s, 1H), 10.56 (s, 1H), 2.30 (m, 2H), 2.14 (m, 2H), 1.61 (m, 4H).
A mixture of 5,6,7,8-tetrahydroquinazolin-2,4-diol (4.85 g, 23.9 mmol) prepared in Step 2 and phosphorus oxychloride (20 ml) was stirred at 130° C. for 3 hours. After cooling the reaction mixture to room temperature, the same was added into ice water and basified with sodium bicarbonate and sodium hydroxide. The aqueous layer was extracted with dichloromethane, and the organic layer was dried on anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=15/1) to give the titled compound (5.1 g) as a white solid.
1H NMR (400 MHz, CDCl3) δ 2.89 (m, 2H), 2.74 (m, 2H), 1.88 (m, 4H).
Diisopropylethylamine (0.86 ml, 4.92 mmol) was added into chloroform (18 ml) solution of 2,4-dichloro-5,6,7,8-tetrahydroquinazoline (1 g, 4.92 mmol) prepared in Reference Example 33 and (S)-3-acetamidopyrrolidine (0.69 g, 5.42 mmol), and then they were stirred at 50° C. overnight. Water was added to the reaction solution and extracted with dichloromethane. The extract was dried with anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=60/1) to give the titled compound (1.14 g) as a white solid.
1H NMR (400 MHz, CD3OD) δ 4.36 (m, 1H), 3.92 (m, 1H), 3.83 (m, 1H), 3.77 (m, 1H), 3.59 (m, 1H), 2.79 (m, 2H), 2.64 (m, 2H), 2.15 (m, 1H), 1.94 (s, 4H), 1.79-1.69 (m, 4H).
The titled compound was prepared as a white solid in the same manner as Reference Example 34 by using 2,4-dichloro-5,6,7,8-tetrahydroquinazoline prepared in Reference Example 33 and 3-((S)-tert-butoxycarbonylamino)pyrrolidine.
1H NMR (400 MHz, CDCl3) δ 4.63 (s, 1H), 4.25 (m, 1H), 3.91 (m, 1H), 3.79-3.70 (m, 2H), 3.54 (m, 1H), 2.72 (m, 4H), 2.17 (m, 1H), 1.88 (m, 1H), 1.78-1.72 (m, 4H), 1.45 (s, 9H); (Yield: 55%).
Diisopropylethylamine (2.7 ml, 15.5 mmol) was added into chloroform (40 ml) solution of 2,4-dichloro-5,6,7,8-tetrahydroquinazoline (1.5 g, 7.39 mmol) prepared in Reference Example 33 and (3S)-(−)-3-(methylamino)-pyrrolidine (0.87 ml, 8.13 mmol), and then they were stirred at 50° C. overnight. Di-tert-butyldicarbonate (1.69 ml, 7.39 mmol) was added thereto, and they were stirred at room temperature overnight. The reaction mixture was diluted in dichloromethane, washed with water, dried with anhydrous magnesium sulfate, and then concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=5/1) to give the titled compound (2.3 g) as a colorless oil.
1H NMR (400 MHz, CDCl3) δ 4.74 (s, 1H), 3.80 (m, 2H), 3.67 (m, 1H), 3.54 (m, 1H), 2.82 (s, 3H), 2.71 (m, 4H), 2.04 (m, 2H), 1.85 (m, 2H), 1.71 (m, 1H), 1.59 (m, 1H), 1.48 (s, 9H).
The titled compound was prepared as a colorless oil in the same manner as Reference Example 36 by using 2,4-dichloro-5,6,7,8-tetrahydroquinazoline prepared in Reference Example 33 and (3S)-(−)-3-(ethylamino)pyrrolidine.
1H NMR (400 MHz, CDCl3) δ 4.56 (s, 1H), 3.80 (m, 2H), 3.68 (m, 1H), 3.54 (m, 1H), 3.28-3.15 (m, 2H), 2.70 (m, 4H), 2.04 (m, 2H), 1.86 (m, 2H), 1.69 (m, 1H), 1.59 (m, 1H), 1.48 (s, 9H), 1.14 (m, 3H); (Yield: 83%).
(R)-(−)-3-aminopiperidine dihydrochloride (940 mg, 5.42 mmol) was added to chloroform (25 ml) solution of 2,4-dichloro-5,6,7,8-tetrahydroquinazoline (1 g, 4.92 mmol) prepared in Reference Example 33 and diisopropylethylamine (3.5 ml, 20.2 mmol), and then they were stirred at 60° C. overnight. Acetyl chloride (0.39 ml, 5.42 mmol) was added thereto at room temperature, and they were stirred for 2 days. The reaction solution was diluted with dichloromethane, washed with water, dried with anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=5/1) to give the titled compound (1.2 g) as a white solid.
1H NMR (400 MHz, CD3OD) δ 7.41 (m, 1H), 7.23 (m, 1H), 7.14 (m, 1H), 4.19-3.98 (m, 5H), 3.15 (m, 2H), 2.49 (m, 1H), 2.46 (m, 3H), 2.30 (s, 3H), 2.25 (m, 1H+3H), 1.36 (m, 3H).
Diisopropylethylamine (3.4 ml, 19.7 mmol) was added into chloroform (25 ml) solution of 2,4-dichloro-5,6,7,8-tetrahydroquinazoline (1 g, 4.92 mmol) prepared in Reference Example 33 and (R)-(−)-3-piperidinecarboxylic acid (0.7 g, 5.42 mmol), and then they were stirred at 60° C. for 2 days. After cooling the reaction solution to room temperature, methylamine hydrochloride (0.33 g, 4.92 mmol), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (0.94 g, 4.92 mmol) and 1-hydroxybenzotriazole hydrate (0.67 g, 4.92 mmol) were added thereto, they were stirred at room temperature overnight. The reaction solution was diluted with dichloromethane, washed with water, dried with anhydrous magnesium sulfate, and concentrated under reduced pressure. The resulting residue was crystallized by using ether/ethyl acetate to give the titled compound (436 mg) as a pale yellow solid.
1H NMR (400 MHz, CDCl3) δ 6.22 (m, 1H), 3.93 (m, 1H), 3.74 (m, 1H), 3.34 (m, 1H), 3.10 (m, 1H), 2.81 (m, 4H), 2.49 (m, 3H), 1.93-1.84 (m, 4H), 1.70-1.60 (m, 4H).
Ethanol/chloroform (15/10 ml) solution of 2,4-dichloroquinazoline (500 mg, 2.5 mmol) prepared in Step 2 of Reference Example 13 and (3S)-(−)-3-acetamidopyrrolidine (480 mg, 3.7 mmol) was stirred at room temperature overnight, and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (ethyl acetate) to give the titled compound (500 mg) as a yellow oil.
1H NMR (400 MHz, CD3OD) δ 8.30 (d, 1H), 7.80-7.70 (m, 1H), 7.63 (d, 1H), 7.55-7.45 (m, 1H), 4.50 (t, 1H), 4.30-4.00 (m, 3H), 3.90-3.80 (m, 1H), 2.35-2.25 (m, 1H), 2.15-2.05 (m, 1H), 1.95 (s, 3H).
Ethanol/chloroform (5/20 ml) solution of 2,4-dichloroquinazoline (500 mg, 2.5 mmol) prepared in Step 2 of Reference Example 13 and (3S)-(−)-3-(methylamino)-pyrrolidine (380 mg, 3.8 mmol) was stirred at room temperature overnight, and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=20/1) to give the titled compound (300 mg) as a yellow solid.
1H NMR (400 MHz, CDCl3) δ 8.12 (d, 1H), 7.80-7.60 (m, 2H), 7.37 (t, 1H), 4.15-3.95 (m, 3H), 3.85-3.75 (m, 1H), 3.45-3.35 (m, 1H), 2.51 (s, 3H), 2.25-2.15 (m, 1H), 2.05-1.95 (m, 1H).
Chloroform (20 ml) solution of 2,4-dichloroquinazoline (500 mg, 2.5 mmol) prepared in Step 2 of Reference Example 13 and (3S)-(−)-3-(ethylamino)pyrrolidine (420 mg, 3.8 mmol) was stirred at room temperature overnight, and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=20/1) to give the titled compound (500 mg) as a yellow solid.
1H NMR (400 MHz, CD3OD) δ 8.31 (d, 1H), 7.75 (t, 1H), 7.62 (d, 1H), 7.48 (t, 1H), 4.20-3.95 (m, 3H), 3.85-3.75 (m, 1H), 3.55-3.45 (m, 1H), 2.80-2.65 (m, 2H), 2.35-2.25 (m, 1H), 2.05-1.95 (m, 1H), 1.17 (t, 3H).
(R)-(−)-3-aminopiperidine dihydrochloride (480 mg, 2.75 mmol) was added into chloroform (20 ml) solution of 2,4-dichloroquinazoline (500 mg, 2.5 mmol) prepared in Step 2 of Reference Example 13 and diisopropylethylamine (1.3 ml, 7.5 mmol), and then the reaction solution was stirred at room temperature overnight and concentrated under reduced pressure. The resulting solution was diluted with dichloromethane, washed with water, dried with anhydrous magnesium sulfate, and concentrated under reduced pressure. The resulting residue was dissolved in dichloromethane (10 ml). Triethylamine (0.35 ml, 2.5 mmol) and acetyl chloride (0.18 ml, 2.5 mmol) were added thereto at 0° C., and the reaction solution was stirred at room temperature overnight and concentrated under reduced pressure. The resulting solution was diluted with dichloromethane, washed with water, dried with anhydrous magnesium sulfate, and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (ethyl acetate) to give the titled compound (110 mg) as a yellow solid.
1H NMR (400 MHz, CDCl3) δ 7.97 (d, 1H), 7.85-7.70 (m, 2H), 7.49 (t, 1H), 6.85 (brs, 1H), 4.20-4.10 (m, 1H), 4.00-3.85 (m, 2H), 3.85-3.70 (m, 2H), 2.03 (s, 3H), 2.00-1.65 (m, 4H).
A mixture of 2-amino-4-methoxybenzoic acid (5 g, 29.9 mmol) and urea (5.4 g, 89.7 mmol) was stirred at 200° C. for 2 hours. After cooling the reaction solution, water was added thereto and the reaction solution was stirred for 1 hour. The resulting brown solid was filtered, washed with water and dried in vacuo to prepare the titled compound (3 g). This compound was used in the subsequent reaction without further purification.
A mixture of 7-methoxyquinazolin-2,4-diol (3 g, 15.6 mmol) prepared in Step 1 and phosphorus oxychloride (10 ml) was stirred at reflux overnight. After cooling the reaction mixture to room temperature, the same was added into ice water, and basified to pH 7-8 by using sodium bicarbonate. The aqueous layer was extracted with dichloromethane, and the organic layer was dried on anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=10/1) to give the titled compound (0.64 g) as a white solid.
1H NMR (400 MHz, CDCl3) δ 8.12 (d, 1H), 7.37-7.20 (m, 2H), 3.99 (s, 3H).
Chloroform (5 ml) solution of 2,4-dichloro-7-methoxyquinazoline (300 mg, 1.3 mmol) prepared in Step 2 and (3S)-(−)-3-acetamidopyrrolidine (250 mg, 1.95 mmol) was stirred at room temperature overnight, and concentrated under reduced pressure. The resulting solution was diluted with ethyl acetate, washed with water, dried with anhydrous magnesium sulfate, and concentrated under reduced pressure to give the titled compound (350 mg) as a white solid.
1H NMR (400 MHz, CD3OD) δ 8.19 (d, 1H), 7.07 (d, 1H), 7.01 (d, 1H), 4.48 (t, 1H), 4.25-3.95 (m, 3H), 3.92 (s, 3H), 3.90-3.80 (m, 1H), 2.30-2.20 (m, 1H), 2.15-2.05 (m, 1H), 1.95 (s, 3H).
Ethanol/chloroform (15/10 ml) solution of 2,4-dichloropyrido[3,2-d]pyrimidine (500 mg, 2.5 mmol) and (3S)-(−)-3-acetamidopyrrolidine (480 mg, 3.7 mmol) was stirred at room temperature overnight, and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (ethyl acetate) to give the titled compound (100 mg) as a yellow oil.
1H NMR (400 MHz, DMSO-d6) δ 8.81 (s, 1H), 8.18 (d, 1H), 8.03 (d, 1H), 7.85-7.75 (m, 1H), 4.50-4.25 (m, 3H), 3.90-3.65 (m, 2H), 2.30-1.80 (m, 5H).
Diisopropylethylamine (2.6 ml, 15 mmol) was slowly added into chloroform (100 ml) solution of 2,4-dichloropyrido[3,2-d]pyrimidine (2 g, 10 mmol) and 3-((S)-tert-butoxycarbonylamino)pyrrolidine (2 g, 11 mmol), and the reaction solution was stirred at room temperature overnight. The resulting solution was washed with water, dried with anhydrous magnesium sulfate, and concentrated under reduced pressure. Ethyl acetate (10 ml) was added to the resulting pale yellow solid, and stirred at room temperature overnight. The resulting white solid was filtered, washed with ethyl acetate and dried in vacuo to give the titled compound (2.6 g).
1H NMR (400 MHz, CD3OD) δ 8.76 (s, 1H), 8.00-7.90 (m, 1H), 7.75-7.65 (m, 1H), 4.70-3.70 (m, 5H), 2.35-1.90 (m, 2H), 1.45 (s, 9H).
Sodium hydride (86 mg, 2.15 mmol, 60 wt %) was added into N,N-dimethylformamide (10 ml) solution of (S)-tert-butyl {1-(2-chloropyrido[3,2-d]pyrimidin-4-yl)pyrrolidin-3-yl}carbamate (0.5 g, 1.43 mmol) prepared in Reference Example 46 at 0° C., and they were stirred for 10 minutes. Methyl iodide (0.11 ml, 1.72 mmol) was added into the reaction solution, and they were stirred at room temperature overnight. Water was added to terminate the reaction, and extracted with ethyl acetate. The extract was dried with anhydrous magnesium sulfate and concentrated under reduced pressure to give the titled compound (0.6 g) as a yellow solid.
1H NMR (400 MHz, CD3OD) δ 8.74 (s, 1H), 7.90 (d, 1H), 7.70-7.65 (m, 1H), 4.80-4.65 (m, 2H), 4.40-4.20 (m, 1H), 4.15-3.95 (m, 1H), 3.80-3.70 (m, 1H), 2.87 (s, 3H), 2.30-2.10 (m, 2H), 1.49 (s, 9H).
Sodium hydride (80 mg, 1.71 mmol, 60 wt %) was added into N,N-dimethylformamide (10 ml) solution of (S)-tert-butyl {1-(2-chloropyrido[3,2-d]pyrimidin-4-yl)pyrrolidin-3-yl}carbamate (0.4 g, 1.14 mmol) prepared in Reference Example 46 at 0° C., and they were stirred for 10 minutes. 1-bromopropane (0.13 ml, 1.37 mmol) was added into the reaction solution, and they were stirred at room temperature for 2 hours. Water was added to terminate the reaction, and extracted with ethyl acetate. The extract was dried with anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=2/1) to give the titled compound (0.3 g) as a white solid.
1H NMR (400 MHz, CDCl3) δ 8.70 (s, 1H), 7.98 (d, 1H), 7.65-7.55 (m, 1H), 4.90-4.55 (m, 2H), 4.30-4.05 (m, 3H), 3.75-3.65 (m, 1H), 3.25-3.00 (m, 2H), 2.30-2.10 (m, 2H), 1.65-1.55 (m, 2H), 1.45 (s, 9H), 0.90 (t, 3H).
Sodium hydride (86 mg, 2.15 mmol, 60 wt %) was added into N,N-dimethylformamide (10 ml) solution of (S)-tert-butyl {1-(2-chloropyrido[3,2-d]pyrimidin-4-yl)pyrrolidin-3-yl}carbamate (0.4 g, 1.14 mmol) prepared in Reference Example 46 at 0° C., and they were stirred for 10 minutes. 1-bromopentane (0.22 ml, 1.72 mmol) was added into the reaction solution, and they were stirred at room temperature for 3 hours. Water was added to terminate the reaction, and extracted with ethyl acetate. The extract was dried with anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=4/1) to give the titled compound (0.4 g) as a white solid.
1H NMR (400 MHz, CDCl3) δ 8.70 (s, 1H), 7.98 (d, 1H), 7.65-7.55 (m, 1H), 4.90-4.55 (m, 2H), 4.30-4.05 (m, 2H), 3.75-3.65 (m, 1H), 3.25-3.05 (m, 2H), 2.25-2.05 (m, 2H), 1.70-1.48 (m, 2H), 1.48 (s, 9H), 1.40-1.20 (m, 4H), 0.90 (t, 3H).
(R)-(−)-3-aminopiperidine dihydrochloride (1.14 g, 6.6 mmol) was added into chloroform (30 ml) solution of 2,4-dichloropyrido[3,2-d]pyrimidine (1.2 g, 6 mmol) and diisopropylethylamine (3.2 ml, 18 mmol), and they were stirred at room temperature overnight. The reaction solution was diluted with dichloromethane, washed with water, dried with anhydrous magnesium sulfate, and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=20/1) to give the titled compound (1 g) as a pale yellow oil.
1H NMR (400 MHz, CD3OD) δ 8.75 (s, 1H), 8.00-7.90 (m, 1H), 7.75-7.65 (m, 1H), 5.50 (brs, 2H), 3.60-3.20 (m, 2H), 3.05-2.95 (m, 1H), 2.15-1.90 (m, 2H), 1.80-1.65 (m, 1H), 1.65-1.45 (m, 1H).
(R)-1-(2-chloropyrido[3,2-d]pyrimidin-4-yl)piperidin-3-amine (0.45 g, 1.7 mmol) prepared in Step 1 was dissolved in dichloromethane (10 ml). Triethylamine (0.48 ml, 3.4 mmol) and acetyl chloride (0.14 ml, 1.87 mmol) were added thereto at 0° C., and they were stirred at room temperature for 4 hours. Water was added to the reaction solution, and the reaction solution was extracted with dichloromethane, dried with anhydrous magnesium sulfate and concentrated under reduced pressure to give the titled compound (0.5 g) as a yellow solid.
1H NMR (400 MHz, CDCl3) δ 8.71 (s, 1H), 8.05 (d, 1H), 7.63 (t, 1H), 6.49 (brs, 1H), 4.67 (brs, 2H), 4.18 (brs, 2H), 3.94 (brs, 1H), 2.10-1.70 (m, 7H).
(R)-1-(2-chloropyrido[3,2-d]pyrimidin-4-yl)piperidin-3-amine (0.54 g, 2.1 mmol) prepared in Step 1 of Reference Example 50 was dissolved in 1,4-dioxane (20 ml). Triethylamine (0.35 ml, 2.5 mmol) and di-tert-butyl dicarbonate (0.53 g, 2.5 mmol) were added thereto at room temperature, and they were stirred overnight and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=2/1) to give the titled compound (0.6 g) as a white solid.
1H NMR (400 MHz, CDCl3) δ 8.70 (s, 1H), 8.10-8.00 (m, 1H), 7.65-7.55 (m, 1H), 5.20-3.70 (m, 5H), 2.05-1.60 (m, 4H), 1.41 (s, 9H).
An aqueous solution (400 ml) of ethyl cyclopentanone-2-carboxylate (30 g, 0.19 mol), 2-methyl-2-thiopseudourea (0.21 mol) and sodium carbonate (20 g, 0.3 mol) was stirred at room temperature for 2 days. The resulting solid was filtered and dried in vacuo to give the titled compound (28 g), and this compound was used in the subsequent reaction without further purification.
A mixture of 2-(methylthio)-6,7-dihydro-5H-cyclopenta-[d]pyrimidin-4-ol (27 g, 0.15 mol) prepared in Step 1, acetic acid (200 ml) and water (90 ml) was stirred at reflux for 3 days. After cooling the reaction mixture to room temperature, the resulting solid was filtered, washed with ethyl acetate and dried in vacuo to give the titled compound (13.3 g) as a pale yellow solid. This compound was used in the subsequent reaction without further purification.
A mixture of 6,7-dihydro-5H-cyclopenta[d]pyrimidin-2,4-diol (13.3 g, 87.4 mmol) prepared in Step 2 and phosphorus oxychloride (100 ml) was stirred at 130° C. overnight. After cooling the reaction mixture to room temperature, the same was added into ice water and basified with sodium bicarbonate. The aqueous layer was extracted with ethyl acetate, and the organic layer was dried on anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=10/1) to give the titled compound (13 g) as a pale yellow solid.
1H NMR (400 MHz, CDCl3) δ 3.09 (t, 2H), 3.00 (t, 2H), 2.23 (t, 2H).
Diisopropylethylamine (0.93 ml, 5.3 mmol) was added into chloroform (20 ml) solution of 2,4-dichloro-6,7-dihydro-5H-cyclopenta[d]pyrimidine (1 g, 5.3 mmol) prepared in Step 3 and (S)-3-acetamidopyrrolidine (0.75 g, 5.8 mmol), and they were stirred at 50° C. overnight. Water was added to the reaction solution, and extracted with dichloromethane. The extract was dried with anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=60/1) of the titled compound (1.3 g) as a white solid.
1H NMR (400 MHz, CDCl3) δ 6.92 (brs, 1H), 4.55 (brs, 1H), 3.85-3.65 (m, 4H), 3.14 (t, 2H), 2.79 (q, 2H), 2.20-2.03 (m, 4H), 2.03 (s, 3H).
Diisopropylethylamine (3.7 ml, 21.2 mmol) was added into chloroform (100 ml) solution of 2,4-dichloro-6,7-dihydro-5H-cyclopenta[d]pyrimidine (4 g, 21.2 mmol) prepared in Step 3 of Reference Example 52 and 3-((S)-tert-butoxycarbonylamino)pyrrolidine (4.3 g, 23.3 mmol), and they were stirred at 50° C. overnight and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=4/1) to give the titled compound (6.1 g) as a white solid.
1H NMR (400 MHz, CDCl3) δ 4.66 (brs, 1H), 4.27 (brs, 1H), 4.00-3.90 (m, 1H), 3.85-3.75 (m, 2H), 3.65-3.55 (m, 1H), 3.10 (t, 2H), 2.82 (t, 2H), 2.25-2.15 (m, 1H), 2.10-2.00 (m, 2H), 2.00-1.90 (m, 1H), 1.45 (s, 9H).
Sodium hydride (0.18 g, 4.43 mmol, 60 wt %) was added into N,N-dimethylformamide (15 ml) solution of (S)-tert-butyl {1-(2-chloro-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl)pyrrolidin-3-yl}carbamate (1.5 g, 2.95 mmol) prepared in Reference Example 53 at 0° C., and they were stirred for 10 minutes. Methyl iodide (0.22 ml, 3.54 mmol) was slowly added to the reaction solution, and they were stirred at room temperature overnight. Water was added to terminate the reaction, and extracted with ethyl acetate. The extract was dried with anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was by purified with silica gel column chromatography (n-hexane/ethyl acetate=4/1) to give the titled compound (1 g) as a pale yellow solid.
1H NMR (400 MHz, CDCl3) δ 4.78 (brs, 1H), 3.95-3.85 (m, 2H), 3.64 (q, 1H), 3.60-3.50 (m, 1H), 3.11 (t, 2H), 2.85-2.75 (m, 5H), 2.20-2.00 (m, 4H), 1.48 (s, 9H).
The titled compound was prepared as a pale yellow solid in the same manner as Reference Example 54 by using (S)-tert-butyl {1-(2-chloro-6,7-dihydro-5H-cyclopenta[d]-pyrimidin-4-yl)pyrrolidin-3-yl}carbamate prepared in Reference Example 53 and 1-bromopropane.
1H NMR (400 MHz, CDCl3) δ 4.53 (brs, 1H), 4.00-3.90 (m, 2H), 3.65-3.50 (m, 2H), 3.20-3.00 (m, 4H), 2.82 (t, 2H), 2.15-2.00 (m, 4H), 1.65-1.50 (m, 2H), 1.45 (s, 9H), 0.89 (s, 3H); (Yield: 90%).
(R)-(−)-3-aminopiperidine dihydrochloride (1 g, 5.82 mmol) was added into chloroform (30 ml) solution of 2,4-dichloro-6,7-dihydro-5H-cyclopenta[d]pyrimidine (1 g, 5.29 mmol) prepared in Step 3 of Reference Example 52 and diisopropylethylamine (3.7 ml, 21.2 mmol), and they were stirred at 60° C. overnight. After cooling to room temperature, acetyl chloride (0.39 ml, 5.42 mmol) was added thereto, and the reaction solution was stirred at room temperature overnight and concentrated under reduced pressure. The resulting residue was diluted with dichloromethane, washed with water, dried with anhydrous magnesium sulfate, and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=4/1) to give the titled compound (1 g) as a white solid.
1H NMR (400 MHz, CDCl3) δ 6.02 (brs, 1H), 4.05-3.85 (m, 3H), 3.55-3.45 (m, 2H), 3.10-3.00 (m, 1H), 2.95-2.80 (m, 3H), 2.07 (q, 2H), 1.98 (s, 3H), 1.97-1.90 (m, 1H), 1.65-1.55 (m, 1H).
(R)-(−)-3-aminopiperidine dihydrochloride (1 g, 5.82 mmol) was added into ethanol (20 ml) solution of 2,4-dichloro-6,7-dihydro-5H-cyclopenta[d]pyrimidine (1 g, 5.29 mmol) prepared in Step 3 of Reference Example 52 and diisopropylethylamine (3 ml, 21.2 mmol), and they were stirred at room temperature overnight and concentrated under reduced pressure. The resulting residue was diluted with dichloromethane, washed with water, dried with anhydrous magnesium sulfate, and concentrated under reduced pressure. The resulting residue was dissolved in chloroform (20 ml), and then diisopropylethylamine (1.4 ml, 8 mmol) and di-tert-butyl dicarbonate (1.35 g, 6.2 mmol) were added thereto, and they were stirred at room temperature overnight. The reaction solution was washed with water, dried with anhydrous magnesium sulfate, and concentrated under reduced pressure. Diethylether (20 ml) was added to the resulting residue, and they were stirred at room temperature for 1 hour. The resulting white solid was filtered to give the titled compound (0.7 g)
1H NMR (400 MHz, CDCl3) δ 4.68 (brs, 1H), 3.95-3.35 (m, 5H), 3.10-2.90 (m, 2H), 2.83 (t, 2H), 2.10-1.90 (m, 3H), 1.85-1.60 (m, 2H), 1.50-1.45 (m, 1H), 1.44 (s, 9H).
Sodium hydride (0.75 mg, 1.88 mmol, 60 wt %) was added into N,N-dimethylformamide (5 ml) solution of (R)-tert-butyl {1-(2-chloro-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl)piperidin-3-yl}carbamate (330 mg, 0.94 mmol) prepared in Reference Example 57 at room temperature, and they were stirred for 10 minutes. Methyl iodide (0.1 ml, 1.41 mmol) was slowly added to the reaction solution, and they were stirred at room temperature overnight. Water was added terminate the reaction, and extracted with ethyl acetate. The extract was dried with anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified silica gel column chromatography (n-hexane/ethyl acetate=2/1) to give the titled compound (280 mg) as a colorless oil.
1H NMR (400 MHz, CDCl3) δ 4.50 (d, 1H), 4.41 (brs, 1H), 3.93 (brs, 1H), 3.15-2.77 (m, 4H), 2.78 (s, 3H), 2.79-2.65 (m, 2H), 2.15-2.00 (m, 2H), 2.00-1.60 (m, 4H), 1.47 (s, 9H).
(R)-(−)-3-aminopiperidine dihydrochloride (1.14 g, 6.6 mmol) was added into chloroform (30 ml) solution of 2,4-dichloro-5,6,7,8-tetrahydroquinazoline (1.2 g, 6 mmol) prepared in Reference Example 33 and diisopropylethylamine (3.2 ml, 18 mmol), and they were stirred at room temperature overnight. The reaction solution was diluted with dichloromethane, washed with water, dried with anhydrous magnesium sulfate, and concentrated under reduced pressure to give the titled compound (1.5 g) as a yellow oil. This compound was used in the subsequent reaction without further purification.
(R)-1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)-piperidin-3-amine (1.5 g, 5.62 mmol) prepared in Step 1 was dissolved in chloroform (20 ml). Diisopropylethylamine (1.5 ml, 8.61 mmol) and di-tert-butyl dicarbonate (1.5 g, 6.87 mmol) were added thereto at room temperature, and the reaction solution was stirred overnight and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=4/1) to give the titled compound (0.6 g) as a white solid. This compound was used in the subsequent reaction without further purification.
Sodium hydride (78.6 mg, 5.46 mmol, 60 wt %) was added into N,N-dimethylformamide (10 ml) solution of (R)-tert-butyl (1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)-piperidin-3-yl)carbamate (0.5 g, 2.73 mmol) prepared in Step 2 at 0° C., and they were stirred for 10 minutes. Methyl iodide (0.25 ml, 4.1 mmol) was slowly added to the reaction solution, and they were stirred at room temperature overnight. Water was added to terminate the reaction, and extracted with ethyl acetate. The extract was dried with anhydrous magnesium sulfate, and then concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=2/1) to give the titled compound (220 mg) as a pale yellow oil.
1H NMR (400 MHz, CDCl3) δ 4.20-3.65 (m, 3H), 2.90-2.50 (m, 7H), 2.51 (brs, 2H), 2.00-1.60 (m, 8H), 1.47 (s, 9H).
Diisopropylethylamine (3.7 ml, 21.2 mmol) was added into chloroform (25 ml) solution of 2,4-dichloro-6,7-dihydro-5H-cyclopenta[d]pyrimidine (1 g, 5.29 mmol) prepared in Step 3 of Reference Example 52 and (R)-(−)-3-piperidincarboxylic acid (0.75 g, 5.82 mmol), and they were stirred at 70° C. overnight. After cooing the reaction solution to room temperature, methylamine hydrochloride (0.36 g, 5.29 mmol), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (1.1 g, 5.82 mmol) and 1-hydroxybenzotriazole hydrate (0.79 g, 5.82 mmol) were added thereto, and they were stirred at room temperature overnight. The reaction solution was diluted with dichloromethane, washed with water, dried with anhydrous magnesium sulfate, and concentrated under reduced pressure. The resulting residue was crystallized by using ethyl acetate to give the titled compound (1.1 g) as a white solid.
1H NMR (400 MHz, CDCl3) δ 6.31 (brs, 1H), 4.21 (d, 1H), 4.07 (d, 1H), 3.60 (t, 1H), 3.30 (t, 1H), 2.97 (t, 2H), 2.95-2.75 (m, 5H), 2.42 (brs, 1H), 2.15-2.00 (m, 3H), 2.00-1.90 (m, 1H), 1.80-1.70 (m, 1H), 1.55 (d, 1H).
A mixture of (S)-tert-butyl {1-(2-chloro-6,7-dimethoxy-quinazolin-4-yl)pyrrolidin-3-yl}carbamate (35 mg, 0.09 mmol) prepared in Reference Example 1, palladium acetate (1 mg, 5 mol %), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthine (5.2 mg, 10 mol %), cesium carbonate (59 mg, 0.18 mmol), 4-chloro-1,3-diaminobenzene (15.7 mg, 0.11 mmol) and 1,4-dioxane (1 ml) was stirred for 1 hour in a microwave (600 W). After cooling the reaction mixture to room temperature, the same was concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1→dichloromethane/methanol=20/1). The resulting residue was dissolved in ethyl acetate, and then hydrochloric acid gas was added thereto. The resulting solid was filtered, washed, dried to prepare the titled compound (1.2 mg) as a pale yellow solid.
1H-NMR (400 MHz, CD3OD) δ 7.62 (brs, 1H), 7.52 (brs, 1H), 7.15 (s, 1H), 6.90 (s, 1H), 6.86 (s, 1H), 4.41 (brs, 3H), 4.26 (brs, 2H), 3.98 (s, 3H), 3.90 (s, 3H), 2.66 (brs, 1H), 2.35 (brs, 1H).
A mixture of (S)-1-(2-chloro-6,7-dimethoxyquinazolin-4-yl)-N-methylpyrrolidin-3-amine (35 mg, 0.09 mmol) prepared in Reference Example 2, palladium acetate (1 mg, 5 mol %), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthine (5.2 mg, 10 mol %), cesium carbonate (59 mg, 0.18 mmol), 5-(trifluoromethyl)-1,3-phenylenediamine (19 mg, 0.11 mmol) and 1,4-dioxane (1 ml) was stirred for 1 hour in a microwave (600 W). After cooling the reaction mixture to room temperature, the same was concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1→dichloromethane/methanol=20/1) to prepare the titled compound (9.2 mg) as a pale yellow solid.
1H-NMR (400 MHz, CD3OD) δ 7.55 (s, 1H), 7.47 (s, 1H), 7.20 (s, 1H), 6.87 (s, 1H), 6.55 (s, 1H), 4.14 (m, 1H), 3.99 (m, 1H), 3.93 (s, 3H), 3.89 (s, 3H), 3.74 (m, 1H), 3.39 (m, 1H), 2.46 (s, 3H), 2.26 (m, 1H), 1.99 (m, 1H).
The titled compounds of Examples 3 and 4 were prepared in the same manner as Example 2 by reacting 3,5-diaminobenzonitrile or 5-(trifluoromethyl)-1,3-phenylenediamine respectively to (S)-1-(2-chloro-6,7-dimethoxy-quinazolin-4-yl)-N-ethylpyrrolidin-3-amine prepared in Reference Example 3.
1H-NMR (400 MHz, CD3OD) δ 7.51 (s, 1H), 7.42 (s, 1H), 7.25 (s, 1H), 6.87 (s, 1H), 6.53 (s, 1H), 4.10 (m, 2H), 3.97 (m, 1H), 3.92 (s, 3H), 3.88 (s, 3H), 3.70 (m, 1H), 3.50 (m, 1H), 2.79 (m, 2H), 2.30 (m, 1H), 1.94 (m, 1H), 1.70 (t, 3H); (Yield: 21%).
1H-NMR (400 MHz, CD3OD) δ 7.50 (s, 1H), 7.41 (s, 1H), 7.20 (s, 1H), 6.84 (s, 1H), 6.56 (s, 1H), 4.12 (m, 2H), 4.00 (m, 1H), 3.91 (s, 3H), 3.88 (s, 3H), 3.76 (m, 1H), 3.53 (m, 1H), 2.80 (m, 2H) 2.31 (m, 1H), 1.99 (m, 1H), 1.19 (t, 3H); (Yield: 20%).
The titled compounds of Examples 5 to 7 were prepared in the same manner as Example 2 by reacting 3,5-diaminobenzonitrile, 5-amino-2-methylbenzonitrile or 5-(trifluoromethyl)-1,3-phenylenediamine respectively with (S)—N-{1-(2-chloro-6,7-dimethoxyquinazolin-4-yl)pyrrolidin-3-yl}acetamide prepared in Reference Example 4.
1H-NMR (400 MHz, CD3OD) δ 7.44 (s, 1H), 7.37 (s, 1H), 7.29 (s, 1H), 6.88 (s, 1H), 6.52 (s, 1H), 4.45 (m, 1H), 4.14 (m, 1H), 4.04 (m, 1H), 3.96 (m, 1H), 3.92 (s, 3H), 3.86 (s, 3H), 3.78 (m, 1H), 2.28 (m, 1H), 2.05 (m, 1H), 1.96 (s, 3H); (Yield: 19%).
1H-NMR (400 MHz, CD3OD) δ 8.23 (d, 1H), 7.72 (d, 1H), 7.43 (s, 1H), 7.26 (d, 1H), 6.91 (s, 1H), 4.88 (m, 1H), 4.18 (m, 1H), 4.10 (m, 1H), 4.07 (m, 1H), 4.00 (s, 3H), 3.96 (s, 3H), 3.82 (m, 1H), 2.45 (s, 3H), 2.28 (m, 1H), 2.07 (m, 1H), 1.93 (s, 3H); (Yield: 24%).
1H-NMR (400 MHz, CD3OD) δ 7.50 (s, 1H), 7.47 (s, 1H), 7.21 (s, 1H), 6.91 (s, 1H), 6.56 (s, 1H), 4.48 (m, 1H), 4.20 (m, 1H), 4.09 (m, 1H), 4.02 (m, 1H), 3.96 (s, 3H), 3.89 (s, 3H), 3.82 (m, 1H), 2.27 (m, 1H), 2.06 (m, 1H), 1.95 (s, 3H); (Yield: 21%).
The titled compounds of Examples 8 to 12 were prepared in the same manner as Example 2 by reacting 5-amino-2-fluorobenzonitrile, 5-amino-2-methylbenzonitrile, 3,5-diaminobenzonitrile, 2-(trifluoromethyl)-1,4-phenylenediamine or 5-(trifluoromethyl)-1,3-phenylenediamine respectively with (R)—N-{1-(2-chloro-6,7-dimethoxyquinazolin-4-yl)-piperidin-3-yl}acetamide prepared in Reference Example 5.
1H-NMR (400 MHz, CD3OD) δ 8.42 (m, 1H), 7.62 (m, 1H), 7.20 (brs, 1H), 7.12 (m, 2H), 7.03 (s, 1H), 5.96 (m, 1H), 4.26 (brs, 1H), 3.80 (m, 1H), 3.33 (m, 1H), 3.12 (m, 1H), 2.05 (m, 1H), 2.02 (s, 3H), 1.90 (m, 1H), 1.82 (m, 3H); (Yield: 24%).
1H-NMR (400 MHz, CD3OD) δ 8.36 (d, 1H), 7.50 (m, 1H), 7.22 (m, 2H), 7.10 (s, 1H), 7.01 (s, 1H), 6.13 (m, 1H), 4.25 (brs, 1H), 4.02 (s, 3H), 4.00 (s, 3H), 3.76 (m, 1H), 3.37 (m, 1H), 3.20 (m, 1H), 2.49 (s, 3H), 2.04 (s+m, 4H), 1.82 (m, 1H), 1.80 (m, 3H), 1.66 (m, 1H); (Yield: 23%).
1H-NMR (400 MHz, CD3OD) δ 7.59 (s, 1H), 7.44 (s, 1H), 7.19 (s, 1H), 7.11 (s, 1H), 7.02 (s, 1H), 6.54 (s, 1H), 6.03 (brs, 1H), 4.24 (brs, 1H), 4.12 (s+m, 7H), 3.91 (m, 1H), 3.77 (brs, 1H), 3.39 (m, 1H), 3.20 (m, 1H), 2.15 (s, 3H), 1.95 (m, 1H), 1.80 (m, 4H), 1.66 (m, 2H); (Yield: 34%).
1H-NMR (400 MHz, CD3OD) δ 7.96 (d, 1H), 7.41 (m, 1H), 7.06 (m, 2H), 6.93 (s, 1H), 6.72 (m, 1H), 6.37 (brs, 1H), 4.20 (brs, 1H), 4.00 (s, 3H), 3.97 (s, 3H), 3.81 (m, 1H), 3.54 (m, 1H), 3.51 (m, 1H), 3.42 (m, 1H), 1.93 (m, 2H), 1.88 (m, 5H), 1.75 (m, 2H); (Yield: 19%).
1H-NMR (400 MHz, CD3OD) δ 7.48 (s, 1H), 7.23 (brs, 1H), 7.14 (m, 1H), 7.09 (s, 1H), 6.98 (s, 1H), 6.54 (s, 1H), 6.19 (m, 1H), 4.24 (m, 1H), 4.01 (s, 3H), 3.99 (s, 3H), 3.88 (m, 1H), 3.75 (m, 1H), 3.47 (m, 1H), 3.32 (m, 1H), 1.99 (m, 1H), 1.88 (m, 6H), 1.75 (m, 2H); (Yield: 23%).
The titled compounds of Examples 13 and 14 were prepared in the same manner as Example 2 by reacting 3,5-diaminobenzonitrile and 5-(trifluoromethyl)-1,3-phenylenediamine respectively with (S)—N-{1-(2-chloro-6-methoxyquinazolin-4-yl)pyrrolidin-3-yl}acetamide prepared in Reference Example 6.
1H-NMR (400 MHz, CD3OD) δ 7.52 (s, 1H), 7.48 (s, 1H), 7.46 (s, 1H), 7.32 (s, 1H), 7.30 (s, 1H), 6.55 (s, 1H), 4.50 (m, 1H), 4.21 (m, 1H), 4.15 (m, 1H), 4.06 (m, 1H), 3.87 (s, 3H), 2.35 (m, 1H), 2.18 (m, 1H), 1.95 (s, 3H); (Yield: 20%).
1H-NMR (400 MHz, CD3OD) δ 7.50 (s, 1H), 7.42 (s, 1H), 7.39 (d, 1H), 7.26 (d, 1H), 7.18 (s, 1H), 6.55 (s, 1H), 4.49 (m, 1H), 4.19 (m, 1H), 4.10 (m, 1H), 3.98 (m, 1H), 3.89 (s, 3H), 2.27 (m, 1H), 2.07 (m, 1H), 1.95 (s, 3H); (Yield: 32%).
The titled compounds of Examples 15 and 16 were prepared in the same manner as Example 2 by reacting 5-(trifluoromethyl)-1,3-phenylenediamine or 3,5-diaminobenzonitrile respectively with (S)-1-(2-chloro-6-methoxy-quinazolin-4-yl)-N-methylpyrrolidin-3-amine prepared in Reference Example 7.
1H-NMR (400 MHz, CD3OD) δ 7.53 (s, 1H), 7.50 (s, 1H), 7.42 (d, 1H), 7.29 (d, 1H), 7.17 (s, 1H), 6.55 (s, 1H), 4.12 (m, 2H), 4.01 (m, 1H), 3.85 (s, 3H), 3.79 (m, 1H), 3.41 (m, 1H), 2.47 (s, 3H), 2.28 (m, 1H), 1.98 (m, 1H); (Yield: 31%).
1H-NMR (400 MHz, CD3OD) δ 7.49 (s, 1H), 7.42 (s, 1H), 7.41-7.39 (m, 1H), 7.26 (m, 1H), 7.24 (s, 1H), 6.52 (s, 1H), 4.08 (m, 2H), 3.92 (m, 1H), 3.83 (s, 3H), 3.73 (m, 1H), 3.39 (m, 1H), 2.47 (s, 3H), 2.26 (m, 1H), 1.96 (m, 1H); (Yield: 21%).
The titled compounds of Examples 17 to 19 were prepared in the same manner as Example 2 by reacting 3,5-diaminobenzonitrile, 5-(trifluoromethyl)-1,3-phenylenediamine or 2-(trifluoromethyl)-1,4-phenylenediamine respectively to (S)-1-(2-chloro-6-methoxyquinazolin-4-yl)-N-ethylpyrrolidin-3-amine prepared in Reference Example 8.
1H-NMR (400 MHz, CD3OD) δ 7.50 (s, 1H), 7.44 (s, 1H), 7.40 (m, 1H), 7.26 (m, 2H), 6.53 (s, 1H), 4.10 (m, 2H), 3.96 (m, 1H), 3.83 (s, 3H), 3.70 (m, 1H), 3.47 (m, 1H), 2.73 (m, 2H), 2.28 (m, 1H), 1.92 (m, 1H), 1.17 (t, 3H); (Yield: 27%).
1H-NMR (400 MHz, CD3OD) δ 7.53 (s, 1H), 7.47 (s, 1H), 7.41 (d, 1H), 7.27 (d, 1H), 7.18 (s, 1H), 6.55 (s, 1H), 4.13 (m, 2H), 3.98 (m, 1H), 3.84 (s, 3H), 3.74 (m, 1H), 3.49 (m, 1H), 2.74 (m, 2H), 2.28 (m, 1H), 1.94 (m, 1H), 1.17 (t, 3H); (Yield: 32%).
1H-NMR (400 MHz, CD3OD) δ 7.94 (s, 1H), 7.50 (s, 1H), 7.45 (d, 1H), 7.37 (d, 1H), 7.28 (d, 1H), 6.81 (d, 1H), 4.11 (m, 2H), 3.95 (m, 1H), 3.85 (s, 3H), 3.75 (m, 1H), 3.50 (m, 1H), 2.74 (m, 2H), 2.28 (m, 1H), 1.95 (m, 1H), 1.18 (t, 3H); (Yield: 34%).
The titled compounds of Examples 20 to 24 were prepared in the same manner as Example 2 by reacting 5-(trifluoromethyl)-1,3-phenylenediamine, 3,5-diaminobenzonitrile, 2,5-diaminobenzonitrile, 5-amino-2-methylbenzonitrile or 2-(trifluoromethyl)-1,4-phenylenediamine respectively with (R)—N-{1-(2-chloro-6-methoxyquinazolin-4-yl)piperidin-3-yl}acetamide prepared in Reference Example 9.
1H-NMR (400 MHz, CD3OD) δ 7.50 (m, 2H), 7.31 (m, 1H), 7.26 (s, 1H), 7.19 (s, 1H), 6.56 (s, 1H), 4.15 (m, 2H), 3.94 (m, 1H), 3.90 (s, 3H), 3.24 (m, 1H), 3.05 (m, 1H), 2.05 (m, 2H), 1.92 (s, 3H), 1.66 (m, 1H), 1.23 (m, 1H); (Yield: 24%).
1H-NMR (400 MHz, CD3OD) δ 7.54 (m, 2H), 7.35 (s, 1H), 7.30 (m, 1H), 7.20 (s, 1H), 6.55 (s, 1H), 4.14 (m, 2H), 3.97 (m, 1H), 3.91 (s, 3H), 3.24 (m, 1H), 3.02 (m, 1H), 2.03 (m, 2H), 1.87 (s, 3H), 1.63 (m, 1H), 1.24 (m, 1H); (Yield: 20%).
1H-NMR (400 MHz, CD3OD) δ 7.88 (s, 1H), 7.55 (d, 1H), 7.45 (d, 1H), 7.27 (d, 1H), 7.16 (s, 1H), 6.80 (d, 1H), 4.10 (m, 2H), 3.97 (m, 1H), 3.89 (s, 3H), 3.19 (m, 1H), 3.01 (m, 1H), 2.04 (m, 2H), 1.92 (s, 3H), 1.80 (m, 1H), 1.62 (m, 1H); (Yield: 34%).
1H-NMR (400 MHz, CD3OD) δ 8.31 (s, 1H), 7.78 (d, 1H), 7.52 (d, 1H), 7.33-7.27 (m, 2H), 7.21 (s, 1H), 4.17 (m, 2H), 3.98 (m, 1H), 3.91 (s, 3H), 3.23 (m, 1H), 3.01 (m, 1H), 2.46 (s, 3H), 2.04 (m, 2H), 1.94 (s, 3H), 1.87 (m, 1H), 1.64 (m, 1H); (Yield: 30%).
1H-NMR (400 MHz, CD3OD) δ 7.94 (s, 1H), 7.47 (d, 1H), 7.41 (m, 1H), 7.30 (d, 1H), 7.19 (s, 1H), 6.82 (d, 1H), 4.10 (m, 2H), 3.95 (m, 1H), 3.90 (s, 3H), 3.22 (m, 1H), 3.02 (m, 1H), 2.04 (m, 1H), 1.92 (s+m, 3+1H), 1.80 (m, 1H), 1.63 (m, 1H); (Yield: 28%).
A mixture of (S)-1-{2-chloro-7-(trifluoromethyl)-quinazolin-4-yl}-N-methylpyrrolidin-3-amine (30 mg, 0.09 mmol) prepared in Reference Example 10 and 5-(trifluoromethyl)-1,3-phenylenediamine (25 mg, 0.14 mmol) was stirred for 1 hour in a microwave (600 W). After cooling the reaction mixture to room temperature, the reaction mixture was concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=1/1→dichloromethane/methanol=20/1). The resulting residue was dissolved in ethyl acetate, and hydrochloric acid gas was added thereto. The resulting solid was filtered, washed and dried to prepare the titled compound (10.1 mg) as a pale yellow solid.
1H-NMR (400 MHz, CD3OD) δ 8.31 (d, 1H), 7.74 (s, 1H), 7.48 (s, 1H), 7.35 (d, 1H), 7.30 (s, 1H), 6.59 (s, 1H), 4.20 (m, 2H), 4.04 (m, 1H), 3.91 (m, 1H), 3.59 (m, 1H), 2.53 (s, 3H), 2.36 (m, 1H), 2.11 (m, 1H).
The titled compounds of Examples 26 and 27 were prepared in the same manner as Example 25 by reacting 5-(trifluoromethyl)-1,3-phenylenediamine or 3,5-diaminobenzonitrile respectively with (S)-1-{2-chloro-7-(trifluoromethyl)quinazolin-4-yl}-N-ethylpyrrolidin-3-amine prepared in Reference Example 11.
1H-NMR (400 MHz, CD3OD) δ 8.30 (d, 1H), 7.71 (s, 1H), 7.49 (s, 1H), 7.35 (d, 1H), 7.29 (s, 1H), 6.59 (s, 1H), 4.18 (m, 2H), 4.09 (m, 1H), 3.83 (m, 1H), 3.61 (m, 1H), 2.77 (m, 2H), 2.31 (m, 1H), 1.98 (m, 1H), 1.16 (t, 3H); (Yield: 19%).
1H-NMR (400 MHz, CD3OD) δ 8.30 (d, 1H), 7.74 (s, 1H), 7.47 (s, 1H), 7.41 (s, 1H), 7.34 (d, 1H), 6.58 (s, 1H), 4.16 (m, 1H), 4.07 (m, 1H), 3.79 (m, 1H), 3.59 (m, 1H), 2.76 (m, 2H), 2.29 (m, 1H), 2.01 (m, 1H), 1.23 (t, 3H); (Yield: 17%).
The titled compounds of Examples 28 and 29 were prepared in the same manner as Example 25 by reacting 3,5-diaminobenzonitrile or 5-(trifluoromethyl)-1,3-phenylenediamine respectively with (S)—N-[1-{2-chloro-7-(trifluoromethyl)quinazolin-4-yl}pyrrolidin-3-yl]acetamide prepared in Reference Example 12.
1H-NMR (400 MHz, CD3OD) δ 8.28 (d, 1H), 7.78 (s, 1H), 7.44 (d, 1H), 7.35 (d, 1H), 6.58 (s, 1H), 4.51 (m, 1H), 4.25 (m, 1H), 4.12 (m, 1H), 4.07 (m, 1H), 3.84 (m, 1H), 2.33 (m, 1H), 2.12 (m, 1H), 1.92 (s, 3H); (Yield: 20%).
1H-NMR (400 MHz, CD3OD) δ 8.29 (d, 1H), 7.73 (d, 1H), 7.48 (s, 1H), 7.35 (d, 1H), 7.32 (s, 1H), 6.59 (s, 1H), 6.28 (s, 1H), 4.52 (m, 1H), 4.26 (m, 1H), 4.15-4.05 (m, 2H), 3.84 (m, 1H), 2.33 (m, 1H), 2.09 (m, 1H), 1.92 (s, 3H); (Yield: 27%).
n-Butanol (1 ml) solution of (S)-tert-butyl 1-(2-chloro-quinazolin-4-yl)pyrrolidin-3-ylcarbamate (20 mg, 0.06 mmol) prepared in Reference Example 13 and 3,5-diaminobenzonitrile (9.2 mg, 0.07 mmol) was stirred for 1 hour in a microwave (400 W). After cooling, the solution was concentrated. The resulting solid was washed with dichloromethane, filtered and dried in vacuo to prepare the titled compound (18 mg) as a white solid.
1H NMR (400 MHz, DMSO-d6) δ 12.68 (brs, 1H), 10.29 (brs, 1H), 8.25 (s, 1H), 7.86 (t, 1H), 7.58 (d, 1H), 7.45 (m, 1H), 7.21 (t, 2H), 6.70 (s, 1H), 5.84 (brs, 2NH), 4.23-3.88 (m, 5H), 2.19 (m, 1H), 2.03 (m, 1H), 1.39 (s, 9H).
Hydrochloric acid gas was added to methanol (2 ml) solution of (S)-tert-butyl 1-{2-(3-amino-5-cyanophenylamino)quinazolin-4-yl}pyrrolidin-3-ylcarbamate hydrochloride (20 mg, 0.04 mmol) prepared in Example 30, and the solution was stirred at room temperature overnight. After concentrating the reaction mixture, it was crystallized with methanol and dichloromethane to prepare the titled compound (14 mg) as a pale yellow solid.
1H NMR (400 MHz, DMSO-d6) δ 12.91 (brs, 1H), 10.49 (brs, 1H), 8.47-8.34 (m, 2H), 7.91 (m, 1H), 7.64-7.52 (m, 1H), 7.17 (m, 2H), 6.74 (s, 1H), 4.55-3.92 (m, 5H), 2.40-2.27 (m, 2H).
The titled compound was prepared as a white solid in the same manner as Example 30 by using (S)-tert-butyl 1-(2-chloroquinazolin-4-yl)pyrrolidin-3-ylcarbamate prepared in Reference Example 13 and 3-(trifluoromethyl)-1,5-phenylenediamine.
1H NMR (400 MHz, DMSO-d6) δ 12.60 (brs, 1H), 10.27 (brs, 1H), 8.25 (s, 1H), 7.84 (m, 1H), 7.57 (m, 1H), 7.46 (m, 1H), 7.33 (m, 1H), 6.96 (s, 1H), 6.66 (s, 1H), 5.88 (brs, 2NH), 4.22-3.73 (m, 5H), 2.19 (m, 1H), 2.02 (m, 1H), 1.39 (s, 9H); (Yield: 56%).
The titled compound was prepared as a pale yellow solid in the same manner as Example 31 by using (S)-tert-butyl 1-[2-{3-amino-5-(trifluoromethyl)phenylamino}quinazolin-4-yl]pyrrolidin-3-ylcarbamate hydrochloride prepared in Step 1.
1H NMR (400 MHz, DMSO-d6) δ 12.91 (brs, 1H), 10.93 (brs, 1H), 8.36-8.16 (m, 2H), 7.92 (m, 1H), 7.64-7.54 (m, 1H), 7.32-7.14 (m, 2H), 6.69 (s, 1H), 4.13-4.06 (m, 5H), 2.41-2.14 (m, 2H); (Yield: 99%).
A mixture of (S)-3-amino-5-{4-(3-aminopyrrolidin-1-yl)-quinazolin-2-ylamino}benzonitrile dihydrochloride (500 mg, 1.20 mmol) prepared in Example 31 and 0.2 N sodium hydroxide solution (10 ml) was stirred at room temperature for 2 hours. The resulting solid was filtered and dried in vacuo at 50° C. to prepare the titled compound (343.5 mg) as a pale yellow solid.
1H NMR (400 MHz, DMSO-d6) δ 9.12 (s, 1H), 8.11 (d, 1H), 7.60 (m, 1H), 7.49 (m, 2H), 7.17 (m, 1H), 6.43 (s, 1H), 5.48 (s, 2NH), 4.03 (m, 2H), 3.90 (m, 1H), 3.60 (m, 2H), 2.06 (m, 1H), 1.72 (m, 1H).
A mixture of (S)-3-amino-5-{4-(3-aminopyrrolidin-1-yl)quinazolin-2-ylamino}benzonitrile (20 mg, 0.06 mmol) prepared in Step 1, propionic acid (4.8 μl, 0.06 mmol), diisopropylethylamine (22.2 μl, 0.13 mmol), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (15.9 mg, 0.08 mmol), 1-hydroxybenzotriazole hydrate (11.2 mg, 0.08 mmol) and N,N-dimethylacetamide (0.5 ml) was stirred at room temperature overnight. Water was added to the reaction mixture, and the resulting solid was washed with 1 N sodium hydroxide aqueous solution, dissolved in methanol, and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=20/1) and dissolved in ethyl acetate (1 ml), and then hydrochloric acid gas was added thereto. The resulting white solid was filtered to prepare the titled compound (4 mg).
1H NMR (400 MHz, CD3OD) δ 8.36 (m, 1H), 7.86 (m, 1H), 7.57 (m, 2H), 7.31 (m, 2H), 6.94 (m, 1H), 4.54-4.07 (m, 5H), 2.34 (m, 1H), 2.24 (m, 2H), 2.11 (m, 1H), 1.10 (m, 3H).
A mixture of (S)-3-amino-5-{4-(3-aminopyrrolidin-1-yl)-quinazolin-2-ylamino}benzonitrile (20 mg, 0.06 mmol) prepared in Step 1 of Example 33, valeric acid (6.9 μl, 0.06 mmol), diisopropylethylamine (22.2 μl, 0.13 mmol), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (15.9 mg, 0.08 mmol), 1-hydroxybenzotriazole hydrate (11.2 mg, 0.08 mmol) and N,N-dimethylacetamide (0.5 ml) was stirred at room temperature overnight. Water was added to the reaction mixture, and the resulting solid was washed with 1 N sodium hydroxide aqueous solution, dissolved in methanol, and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=20/1) to prepare the titled compound (15.2 mg) as a colorless oil.
1H NMR (400 MHz, CD3OD) δ 8.08 (d, 1H), 7.56 (m, 1H), 7.49 (m, 2H), 7.34 (s, 1H), 7.18 (t, 1H), 6.56 (s, 1H), 4.48 (m, 1H), 4.21-4.00 (m, 3H), 3.81 (m, 1H), 2.29 (m, 1H), 2.19 (m, 2H), 2.06 (m, 1H), 1.58 (m, 2H), 1.31 (m, 2H), 0.90 (t, 3H).
The titled compounds of Examples 35 to 38 were prepared in the same manner as Example 34 by reacting phenylacetic acid, 3-phenylpropionic acid, 2-(thiophen-2-yl)acetic acid or N,N-dimethylglycine respectively with (S)-3-amino-5-{4-(3-aminopyrrolidin-1-yl)quinazolin-2-ylamino}benzonitrile prepared in Step 1 of Example 33.
1H NMR (400 MHz, CD3OD) δ 8.08 (d, 1H), 7.58 (m, 1H), 7.50 (m, 2H), 7.33 (s, 1H), 7.24 (m, 4H), 7.18 (m, 2H), 6.56 (s, 1H), 4.47 (m, 1H), 4.18-4.00 (m, 3H), 3.85 (m, 1H), 3.50 (s, 2H), 2.28 (m, 1H), 2.09 (m, 1H); (Yield: 40%).
1H NMR (400 MHz, CD3OD) δ 8.03 (d, 1H), 7.59 (t, 1H), 7.49 (m, 2H), 7.32 (s, 1H), 7.18 (t, 1H), 7.12 (m, 4H), 7.02 (m, 1H), 6.56 (s, 1H), 4.40 (m, 1H), 4.09 (m, 1H), 3.88 (m, 2H), 3.70 (m, 1H), 2.89 (t, 2H), 2.47 (t, 2H), 2.17 (m, 1H), 1.96 (m, 1H); (Yield: 46%).
1H NMR (400 MHz, CD3OD) δ 8.11 (d, 1H), 7.58 (m, 1H), 7.50-7.47 (m, 2H), 7.34 (s, 1H), 7.21-7.18 (m, 2H), 6.90 (m, 2H), 6.56 (s, 1H), 4.48 (m, 1H), 4.22-3.86 (m, 4H), 3.71 (s, 2H), 2.29 (m, 1H), 2.11 (m, 1H); (Yield: 16%).
1H NMR (400 MHz, CD3OD) δ 8.07 (d, 1H), 7.55 (t, 1H), 7.47 (m, 2H), 7.30 (s, 1H), 7.15 (t, 1H), 6.55 (s, 1H), 4.54 (m, 1H), 4.18 (m, 1H), 4.07 (m, 1H), 3.84 (m, 1H), 3.81 (m, 1H), 3.01 (s, 2H), 2.28 (m, 6H+1H), 2.10 (m, 1H); (Yield: 47%).
Propionaldehyde (4.6 μl, 0.06 mmol) was added into methanol (1 ml) solution of (S)-3-amino-5-{4-(3-aminopyrrolidin-1-yl)quinazolin-2-ylamino}benzonitrile (20 mg, 0.06 mmol) prepared in Step 1 of Example 33, and they were stirred at room temperature for 1 hour, and sodium triacetoxyborohydride (24.5 mg, 0.12 mmol) was added thereto. The reaction solution was stirred at room temperature overnight, and then water was added to terminate the reaction. The reaction solution was extracted by adding chloroform and washed with saturated sodium bicarbonate aqueous solution. The extract was dried with anhydrous magnesium sulfate and filtered, and the solution was concentrated. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=20/1) and dissolved in ethyl acetate (1 ml), and hydrochloric acid gas was added thereto. The resulting white solid was filtered to prepare the titled compound (5 mg).
1H NMR (400 MHz, CD3OD) δ 8.36 (m, 1H), 7.90 (t, 1H), 7.62 (d, 1H), 7.56 (t, 1H), 7.49 (s, 1H), 7.36 (s, 1H), 7.03 (s, 1H), 4.53-4.12 (m, 5H), 3.14 (m, 2H), 2.64 (m, 1H), 2.44 (m, 1H), 1.79 (m, 2H), 1.07 (t, 3H).
Butyraldehyde (8.6 μl, 0.10 mmol) was added into methanol (1 ml) solution of (S)-3-amino-5-{4-(3-aminopyrrolidin-1-yl)quinazolin-2-ylamino}benzonitrile (30 mg, 0.09 mmol) prepared in Step 1 of Example 33, and they were stirred at room temperature for 1 hour, and sodium triacetoxyborohydride (24.5 mg, 0.12 mmol) was added thereto. The reaction solution was stirred at room temperature overnight, and water was added to terminate the reaction. The reaction solution was extracted by adding chloroform and washed with saturated sodium bicarbonate aqueous solution. The extract was dried with anhydrous magnesium sulfate and filtered, and the solution was concentrated. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=20/1) to prepare the titled compound (2.7 mg) as a colorless oil.
1H NMR (400 MHz, CD3OD) δ 8.12 (d, 1H), 7.58 (m, 1H), 7.52-7.47 (m, 2H), 7.29 (s, 1H), 7.18 (m, 1H), 6.56 (s, 1H), 4.14 (m, 2H), 4.01 (m, 1H), 3.79 (m, 1H), 3.54 (m, 1H), 2.75 (m, 2H), 2.31 (m, 1H), 1.99 (m, 1H), 1.56 (m, 2H), 1.40 (m, 2H), 0.96 (m, 3H).
The titled compounds of Examples 41 to 51 were prepared in the same manner as Example 40 by reacting valeraldehyde, isovaleraldehyde, cyclopropane carboxaldehyde, pivalaldehyde, benzaldehyde, acetone, methylethylketone, 2-pentanone, 2-hexanone, 5-methyl-2-hexanone or cyclohexanone respectively with (S)-3-amino-5-{4-(3-aminopyrrolidin-1-yl)quinazolin-2-ylamino}benzonitrile prepared in Step 1 of Example 33.
1H NMR (400 MHz, CD3OD) δ 8.17 (d, 1H), 7.57 (m, 1H), 7.52-7.47 (m, 2H), 7.30 (s, 1H), 7.21 (t, 1H), 6.56 (s, 1H), 4.15 (m, 2H), 4.03 (m, 1H), 3.77 (m, 1H), 3.48 (m, 1H), 2.71 (t, 2H), 2.30 (m, 1H), 1.97 (m, 1H), 1.57 (m, 2H), 1.36 (m, 4H), 0.93 (m, 3H); (Yield: 3%).
1H NMR (400 MHz, CD3OD) δ 8.13 (d, 1H), 7.58 (t, 1H), 7.51-7.47 (m, 2H), 7.31 (s, 1H), 7.19 (t, 1H), 6.56 (s, 1H), 4.14 (m, 2H), 4.01 (m, 1H), 3.78 (m, 1H), 3.54 (m, 1H), 2.74 (m, 2H), 2.31 (m, 1H), 1.98 (m, 1H), 1.67 (m, 1H), 1.46 (m, 2H), 0.94 (d, 6H); (Yield: 6%).
1H NMR (400 MHz, CD3OD) δ 8.13 (d, 1H), 7.58 (m, 1H), 7.52-7.49 (m, 2H), 7.30 (s, 1H), 7.19 (m, 1H), 6.56 (s, 1H), 4.15 (m, 2H), 4.02 (m, 1H), 3.80 (m, 1H), 3.58 (m, 1H), 2.61 (d, 2H), 2.31 (m, 1H), 2.01 (m, 1H), 1.01 (m, 1H), 0.56 (m, 2H), 0.24 (m, 2H); (Yield: 6%).
1H NMR (400 MHz, CD3OD) δ 8.16 (d, 1H), 7.58 (m, 2H), 7.47 (d, 1H), 7.30 (s, 1H), 7.19 (t, 1H), 6.56 (s, 1H), 4.15 (m, 2H), 3.97 (m, 1H), 3.76 (m, 1H), 3.44 (m, 1H), 2.48 (s, 2H), 2.24 (m, 1H), 1.95 (m, 1H), 0.95 (s, 9H); (Yield: 4%)
1H NMR (400 MHz, CD3OD) δ 8.08 (d, 1H), 7.60 (m, 1H), 7.52 (s, 1H), 7.46 (d, 1H), 7.39 (m, 2H), 7.31 (m, 3H), 7.25 (m, 1H), 7.17 (m, 1H), 6.57 (s, 1H), 4.14 (m, 2H), 3.99 (m, 1H), 3.87 (s, 2H), 3.80 (m, 1H), 3.49 (m, 1H), 2.30 (m, 1H), 2.03 (m, 1H); (Yield: 3%)
1H NMR (400 MHz, CD3OD) δ 8.16 (d, 1H), 7.63 (t, 1H), 7.55 (m, 2H), 7.23 (m, 2H), 6.59 (s, 1H), 4.29-4.12 (m, 3H), 4.12-3.94 (m, 2H), 2.50 (m, 1H), 2.15 (m, 1H), 1.32 (m, 6H), 0.90 (m, 1H); (Yield: 1%)
1H NMR (400 MHz, CD3OD) δ 8.14 (d, 1H), 7.57 (t, 1H), 7.50 (m, 2H), 7.27 (m, 1H), 7.20 (m, 1H), 6.57 (s, 1H), 4.19 (m, 2H), 4.04 (m, 1H), 3.77 (m, 2H), 2.91 (m, 1H), 2.36 (m, 1H), 2.00 (m, 1H), 1.68 (m, 1H), 1.42 (m, 1H), 1.17 (m, 3H), 0.98 (m, 3H); (Yield: 3%)
1H NMR (400 MHz, CD3OD) δ 8.12 (d, 1H), 7.56 (t, 1H), 7.49 (m, 2H), 7.29 (m, 1H), 7.18 (m, 1H), 6.56 (s, 1H), 4.14 (m, 2H), 4.01 (m, 1H), 3.74 (m, 2H), 2.94 (m, 1H), 2.34 (m, 1H), 1.95 (m, 1H), 1.58 (m, 1H), 1.38 (m, 3H), 1.17 (m, 3H), 0.95 (m, 3H); (Yield: 6%).
1H NMR (400 MHz, CD3OD) δ 8.10 (d, 1H), 7.57 (t, 1H), 7.47 (m, 2H), 7.29 (m, 1H), 7.16 (m, 1H), 6.55 (s, 1H), 4.11 (m, 2H), 3.97 (m, 1H), 3.69 (m, 2H), 2.87 (m, 1H), 2.31 (m, 1H), 1.93 (m, 1H), 1.60 (m, 1H), 1.35 (m, 5H), 1.15 (m, 3H), 0.94 (m, 3H); (Yield: 10%)
1H NMR (400 MHz, CD3OD) δ 8.11 (d, 1H), 7.52 (t, 1H), 7.47 (m, 2H), 7.30 (m, 1H), 7.18 (m, 1H), 6.56 (s, 1H), 4.14 (m, 2H), 3.99 (m, 1H), 3.73 (m, 2H), 2.87 (m, 1H), 2.33 (m, 1H), 1.96 (m, 1H), 1.58 (m, 2H), 1.36 (m, 1H), 1.25 (m, 2H), 1.16 (m, 3H), 0.91 (m, 6H); (Yield: 5%)
1H NMR (400 MHz, CD3OD) δ 8.10 (d, 1H), 7.55 (t, 1H), 7.49 (m, 2H), 7.28 (s, 1H), 7.16 (m, 1H), 6.55 (s, 1H), 4.12 (m, 2H), 3.96 (m, 1H), 3.70 (m, 2H), 2.70 (m, 1H), 2.31 (m, 1H), 2.02-1.92 (m, 3H), 1.77 (m, 2H), 1.66 (m, 1H), 1.37-1.13 (m, 5H); (Yield: 21%)
n-Butanol (1.5 ml) solution of (S)-tert-butyl 1-(2-chloroquinazolin-4-yl)pyrrolidin-3-yl(propyl)carbamate (57.7 mg, 0.15 mmol) prepared in Reference Example 14 and 3-(trifluoromethyl)-1,5-phenylenediamine (31.2 mg, 0.18 mmol) was stirred at 130° C. overnight. After cooling the reaction solution, the same was concentrated under reduced pressure. The resulting residue was crystallized with n-butanol/dichloromethane and dried under reduced pressure. The resulting solid was dissolved in methanol (2 ml), and then hydrochloric acid gas was added thereto. The resulting white solid was filtered to prepare the titled compound (33.4 mg).
1H NMR (400 MHz, DMSO-d6) δ 13.17 (brs, 1H), 10.69 (s, 1H), 9.77-9.54 (m, 2H), 8.29 (s, 1H), 7.90 (t, 1H), 7.61 (d, 1H), 7.53 (t, 1H), 7.40-7.18 (m, 2H), 6.78 (s, 1H), 4.51-4.01 (m, 5H), 2.98 (m, 2H), 2.45 (m, 2H), 1.71 (m, 2H), 1.04 (m, 3H)
The titled compound was prepared as a white solid in the same manner as Example 52 by using (S)-tert-butyl butyl{1-(2-chloroquinazolin-4-yl)pyrrolidin-3-yl}carbamate prepared in Reference Example 15.
1H NMR (400 MHz, DMSO-d6) δ 13.03 (brs, 1H), 10.66 (s, 1H), 9.76-9.33 (m, 2H), 8.50 (s, 1H), 7.91 (t, 1H), 7.60 (d, 1H), 7.53 (t, 1H), 7.40-7.14 (m, 2H), 6.74 (s, 1H), 4.29-4.01 (m, 5H), 3.01 (m, 2H), 2.40 (m, 2H), 1.66 (m, 2H), 1.36 (m, 2H), 0.92 (m, 3H); (Yield: 46%)
The titled compound was prepared as a white solid in the same manner as Example 52 by using (S)-tert-butyl 1-(2-chloroquinazolin-4-yl)pyrrolidin-3-yl(pentyl)carbamate prepared in Reference Example 16.
1H NMR (400 MHz, DMSO-d6) δ 13.15 (brs, 1H), 10.75 (s, 1H), 9.77-9.39 (m, 2H), 8.29 (s, 1H), 7.92 (t, 1H), 7.60 (d, 1H), 7.53 (t, 1H), 7.43-7.21 (m, 2H), 6.82 (s, 1H), 4.52-4.30 (m, 5H), 3.00 (m, 2H), 2.44 (m, 2H), 1.69 (m, 2H), 1.32 (m, 4H), 0.89 (m, 3H); (Yield: 43%)
The titled compound was prepared as a white solid in the same manner as Example 52 by using (S)-tert-butyl 1-(2-chloroquinazolin-4-yl)pyrrolidin-3-yl(pentyl)carbamate which was prepared in Reference Example 16 and 3,5-diaminobenzonitrile.
1H NMR (400 MHz, DMSO-d6) δ 13.09 (brs, 1H), 10.64 (s, 1H), 9.75-9.28 (m, 2H), 8.29 (s, 1H), 7.90 (t, 1H), 7.60 (d, 1H), 7.50 (t, 1H), 7.27-7.15 (m, 2H), 6.77 (s, 1H), 4.50-4.22 (m, 5H), 3.01 (m, 2H), 2.44 (m, 1H), 2.00 (m, 2H), 1.18 (m, 4H), 0.89 (m, 3H); (Yield: 74%)
The titled compound was prepared as a white solid in the same manner as Example 52 by using (S)-tert-butyl 1-(2-chloroquinazolin-4-yl)pyrrolidin-3-yl(hexyl)carbamate prepared in Reference Example 17 and 3,5-diaminobenzonitrile.
1H NMR (400 MHz, DMSO-d6) δ 10.85 (s, 1H), 9.80-9.54 (m, 2H), 8.29 (s, 1H), 7.90 (t, 1H), 7.59 (d, 1H), 7.40 (t, 1H), 7.27-7.12 (m, 2H), 6.77 (s, 1H), 4.35-4.06 (m, 5H), 3.01 (m, 2H), 2.46 (m, 2H), 1.68 (m, 2H), 1.34 (m, 6H), 0.88 (m, 3H); (Yield: 79%)
A mixture of (S)—N-{1-(2-chloro-8-methoxyquinazolin-4-yl)-pyrrolidin-3-yl}acetamide (30 mg, 0.09 mmol) prepared in Reference Example 19 and 2,5-diaminobenzonitrile (15 mg, 0.11 mmol) was stirred for 40 minutes in a microwave (400 W). After cooling the reaction solution to room temperature, the same was basified with sodium bicarbonate aqueous solution and extracted with dichloromethane. The extract was dried with anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=30/1) to prepare the titled compound (1.4 mg) as a pale yellow oil.
1H NMR (400 MHz, CD3OD) δ 7.95 (s, 1H), 7.71 (d, 1H), 7.49 (d, 1H), 7.16 (m, 2H), 6.80 (d, 1H), 4.56 (m, 1H), 4.21-4.00 (m, 3H+3H), 3.83 (m, 1H), 2.27 (m, 1H), 2.06 (m, 1H), 1.94 (s, 3H)
The titled compound was prepared as a pale yellow oil in the same manner as Example 57 by using (S)—N-{1-(2-chloro-8-methoxyquinazolin-4-yl)-pyrrolidin-3-yl}acetamide prepared in Reference Example 19 and 3-(trifluoromethyl)-1,5-phenylenediamine.
1H NMR (400 MHz, CDCl3) δ 7.75 (s, 1H), 7.56 (s, 1H), 7.11-7.00 (m, 2H), 6.87 (s, 1H), 6.51 (s, 1H), 5.85 (brs, 1H), 4.64 (m, 1H), 4.23 (m, 1H), 4.00 (3H+1H), 3.85 (m, 2H), 2.34 (m, 1H), 2.02 (m, 1H+3H); (Yield: 15%)
(3S)-(−)-3-(methylamino)pyrrolidine (0.17 ml, 1.57 mmol) was added to ethanol/chloroform (10/10 ml) solution of 2,4-dichloro-8-methoxyquinazoline (300 mg, 1.31 mmol) prepared in Reference Example 18, and they were stirred at room temperature overnight. The reaction solution was concentrated under reduced pressure, dissolved in dichloromethane, washed with water, dried with anhydrous magnesium sulfate, and concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=20/1) to prepare the titled compound (371 mg) as a yellow oil.
1H NMR (400 MHz, CDCl3) δ 7.70 (d, 1H), 7.28 (t, 1H), 7.08 (d, 1H), 4.13 (m, 2H), 4.05 (m, 1H+3H), 3.75 (m, 1H), 3.40 (m, 1H), 2.50 (s, 3H), 2.17 (m, 1H), 1.94 (m, 1H)
A mixture of (S)-{1-(2-chloro-8-methoxyquinazolin-4-yl)-pyrrolidin-3-yl}methylamine (20 mg, 0.07 mmol) prepared in Step 1, 3-(trifluoromethyl)-1,5-phenylenediamine (14 mg, 0.08 mmol), palladium acetate (0.77 mg, 0.003 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthine (4.0 mg, 0.01 mmol), cesium carbonate (44.5 mg, 0.14 mmol) and anhydrous 1,4-dioxane (1 ml) was stirred for 1 hour in a microwave (600 W). After cooling the reaction solution to room temperature, the same was filtered by celite. The filtrate was concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=10/1) to prepare the titled compound (16.7 mg) as a pale yellow oil.
1H NMR (400 MHz, CD3OD) δ 7.63 (m, 2H), 7.04 (m, 3H), 6.56 (m, 1H), 4.03-3.86 (m, 6H), 3.69 (m, 1H), 2.43 (s, 3H), 2.21 (m, 1H), 1.89 (m, 1H)
The titled compounds of Examples 60 to 62 were prepared in the same manner as Example 57 by reacting 2-nitro-1,4-phenylenediamine, 3,5-diaminobenzonitrile or 3-(trifluoromethyl)-1,5-phenylenediamine respectively with (S)-{1-(2-chloro-8-methoxyquinazolin-4-yl)-pyrrolidin-3-yl}ethylamine prepared in Reference Example 20.
1H NMR (400 MHz, CD3OD) δ 8.92 (s, 1H), 7.73 (d, 1H), 7.38 (d, 1H), 7.11 (m, 2H), 6.90 (d, 1H), 4.15 (m, 2H), 4.04-3.98 (m, 1H+3H), 3.84 (m, 1H), 3.55 (m, 1H), 2.79 (m, 2H), 2.33 (m, 1H), 1.98 (m, 1H), 1.19 (m, 3H); (Yield: 5%)
1H NMR (400 MHz, CD3OD) δ 7.65 (m, 2H), 7.13-7.10 (m, 3H), 6.54 (s, 1H), 4.08-3.96 (m, 6H), 3.70 (m, 1H), 3.47 (m, 1H), 2.74 (m, 2H), 2.27 (m, 1H), 1.90 (m, 1H), 1.17 (m, 3H); (Yield: 15%)
1H NMR (400 MHz, CD3OD) δ 7.67-7.63 (m, 1H+1H), 7.05 (m, 3H), 6.56 (s, 1H), 4.08 (m, 2H), 4.04-3.86 (m, 3H+1H), 3.67 (m, 1H), 3.42 (m, 1H), 2.71 (m, 2H), 2.24 (m, 1H), 1.87 (m, 1H), 1.15 (t, 3H); (Yield: 17%)
The titled compounds of Examples 63 to 65 were prepared in the same manner as Step 2 of Example 59 by reacting 3,5-diaminobenzonitrile, 3-(trifluoromethyl)-1,5-phenylenediamine or 2-(trifluoromethyl)-1,4-phenylenediamine respectively with (R)—N-{1-(2-chloro-8-methoxyquinazolin-4-yl)piperidin-3-yl}acetamide prepared in Reference Example 21.
1H NMR (400 MHz, CD3OD) δ 7.70 (s, 1H), 7.45 (d, 1H), 7.19-7.13 (m, 3H), 6.57 (s, 1H), 4.25 (d, 1H), 4.09 (m, 2H), 3.98 (s, 3H), 3.31 (m, 1H), 3.11 (m, 1H), 2.06 (m, 1H), 1.93 (m, 3H+1H), 1.82 (m, 1H), 1.60 (m, 1H); (Yield: 12%)
1H NMR (400 MHz, CD3OD) δ 7.72 (s, 1H), 7.46 (d, 1H), 7.16-7.10 (m, 3H), 6.58 (s, 1H), 4.24 (m, 1H), 4.09-3.98 (m, 5H), 3.50 (m, 1H), 3.14 (m, 1H), 2.02 (m, 1H), 1.91 (m, 3H+1H), 1.81 (m, 1H), 1.62 (m, 1H); (Yield: 15%)
1H NMR (400 MHz, CD3OD) δ 8.15 (m, 1H), 7.43 (m, 2H), 7.10 (m, 2H), 6.81 (m, 1H), 4.17 (m, 1H), 4.00-3.92 (m, 5H), 3.30 (m, 1H), 3.09 (m, 1H), 2.00 (m, 1H), 1.86 (m, 4H), 1.77 (m, 1H), 1.61 (m, 1H); (Yield: 17%)
The titled compounds of Examples 66 and 67 were prepared in the same manner as Step 2 of Example 59 by reacting 3,5-diaminobenzonitrile or 3-(trifluoromethyl)-1,5-phenylenediamine respectively with (S)—N-{1-(2-chloro-5-methylquinazolin-4-yl)-pyrrolidin-3-yl}acetamide prepared in Reference Example 23.
1H NMR (400 MHz, CDCl3) δ 7.44-7.27 (m, 3H), 7.27 (m, 1H), 7.01 (m, 1H), 6.51 (m, 1H), 5.87 (m, 1H), 4.47 (m, 1H), 3.92 (m, 3H), 3.78-3.54 (m, 3H), 2.62 (s, 3H), 2.24 (m, 1H), 1.91 (s, 3H+1H); (Yield: 36%)
1H NMR (400 MHz, CDCl3) δ 7.51-7.44 (m, 2H), 7.35 (m, 1H), 7.23 (m, 1H), 6.99 (m, 1H), 6.53 (m, 1H), 5.82 (m, 1H), 4.47 (m, 1H), 3.87 (m, 3H), 3.75-3.57 (m, 3H), 2.62 (m, 3H), 2.24 (m, 1H), 1.85 (m, 3H+1H); (Yield: 27%)
The titled compound was prepared as a yellow oil in the same manner as Step 3 of Reference Example 13 by using 2,4-dichloro-5-methylquinazoline prepared in Reference Example 22 and (3S)-(−)-3-(methylamino)pyrrolidine.
1H NMR (400 MHz, CDCl3) δ 7.56 (m, 2H), 7.24 (m, 1H), 4.08 (m, 1H), 4.04 (m, 2H), 3.83 (m, 1H), 3.55 (m, 1H), 2.65 (s, 3H), 2.62 (s, 3H), 2.22-2.12 (m, 2H); (Yield: 51%)
t-Butanol (0.5 ml) solution of (S)-1-(2-chloro-5-methylquinazolin-4-yl)-N-methylpyrrolidin-3-amine (20 mg, 0.07 mmol) prepared in Step 1 and 3,5-diaminobenzonitrile (19 mg, 0.14 mmol) was stirred for 1 hour in a microwave (300 W). After cooling the reaction solution to room temperature, diisopropylethylamine was added thereto and the reaction solution was concentrated. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=20/1) to prepare the titled compound (0.7 mg) as a yellow oil.
1H NMR (400 MHz, CD3OD) δ 7.49 (m, 2H), 7.29 (m, 2H), 7.09 (m, 1H), 6.59 (s, 1H), 3.93-3.42 (m, 5H), 2.65 (s, 3H), 2.46 (s, 3H), 2.23 (m, 1H), 1.84 (m, 1H)
The titled compounds of Examples 69 and 70 were prepared in the same manner as Step 2 of Example 68 by reacting 3-(trifluoromethyl)-1,5-phenylenediamine or 2-(trifluoromethyl)-1,4-phenylenediamine respectively with (S)-1-(2-chloro-5-methylquinazolin-4-yl)-N-ethylpyrrolidin-3-amine prepared in Reference Example 24.
1H NMR (400 MHz, CD3OD) δ 7.49 (m, 2H), 7.28 (m, 1H), 7.20 (s, 1H), 7.04 (m, 1H), 6.58 (s, 1H), 3.89-3.42 (m, 5H), 2.69-2.64 (m, 2H+3H), 2.19 (m, 1H), 1.78 (m, 1H), 1.13 (t, 3H); (Yield: 5%)
1H NMR (400 MHz, CD3OD) δ 7.92 (m, 1H), 7.45 (m, 2H), 7.19 (m, 1H), 6.99 (m, 1H), 6.83 (m, 1H), 3.83-3.48 (m, 5H), 2.61 (m, 2H+3H), 2.16 (m, 1H), 1.76 (m, 1H), 1.11 (m, 3H); (Yield: 13%)
t-Butanol (1 ml) solution of (R)—N-{1-(2-chloro-5-methylquinazolin-4-yl)piperidin-3-yl}acetamide (20 mg, 0.06 mmol) prepared in Reference Example 25 and 3,5-diaminobenzonitrile (21 mg, 0.15 mmol) was stirred under reflux overnight. After cooling the reaction solution to room temperature, diisopropylethylamine was added thereto and the reaction solution was concentrated. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=50/1) to prepare the titled compound (7.7 mg) as a pale red solid.
1H NMR (400 MHz, CD3OD) δ 7.52 (s, 1H), 7.48 (m, 1H), 7.37 (m, 1H), 7.31 (s, 1H), 7.05 (m, 1H), 6.58 (s, 1H), 4.12-3.64 (m, 3H), 3.13 (m, 1H), 2.90 (m, 1H), 2.76 (s, 3H), 1.95 (m, 2H), 1.82 (s, 3H), 1.68-1.51 (m, 2H)
The titled compound was prepared as a yellow oil in the same manner as Step 2 of Example 68 by using (R)—N-{1-(2-chloro-5-methylquinazolin-4-yl)piperidin-3-yl}acetamide prepared in Reference Example 25 and 3-(trifluoromethyl)-1,5-phenylenediamine.
1H NMR (400 MHz, CD3OD) δ 7.49 (m, 2H), 7.33 (m, 1H), 7.23-7.14 (m, 1H), 7.06 (m, 1H), 4.16-3.62 (m, 3H), 3.19-3.05 (m, 2H), 2.72 (s, 3H), 1.98 (m, 2H), 1.75 (s, 3H), 1.56 (m, 2H); (Yield: 71%)
t-Butanol (0.5 ml) solution of (S)—N-{1-(2-chloro-8-methylquinazolin-4-yl)pyrrolidin-3-yl}acetamide (20 mg, 0.07 mmol) prepared in Reference Example 27, 3,5-diaminobenzonitrile (17 mg, 0.13 mmol) and diisopropylethylamine (14 μl, 0.08 mmol) was stirred for 1 hour in a microwave (500 W). After cooling the reaction solution to room temperature, the same was concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=30/1) to prepare the titled compound (1.3 mg) as a pale yellow oil.
1H NMR (400 MHz, CD3OD) δ 7.96 (d, 1H), 7.83 (s, 1H), 7.49 (d, 1H), 7.34 (s, 1H), 7.08 (t, 1H), 6.56 (s, 1H), 4.47 (m, 1H), 4.22 (m, 1H), 4.11 (m, 1H), 4.04 (m, 1H), 3.80 (m, 1H), 2.58 (s, 3H), 2.27 (m, 1H), 2.07 (m, 1H), 1.95 (s, 3H)
The titled compound was prepared as a pale yellow oil in the same manner as Example 73 by using (S)—N-{1-(2-chloro-8-methylquinazolin-4-yl)pyrrolidin-3-yl}acetamide prepared in Reference Example 27 and 3-(trifluoromethyl)-1,5-phenylenediamine.
1H NMR (400 MHz, CD3OD) δ 7.96 (m, 2H), 7.46 (d, 1H), 7.15 (s, 1H), 7.05 (t, 1H), 6.56 (s, 1H), 4.47 (m, 1H), 4.19 (m, 1H), 4.11 (m, 1H), 4.01 (m, 1H), 3.80 (m, 1H), 2.57 (s, 3H), 2.24 (m, 1H), 2.07 (m, 1H), 1.95 (s, 3H); (Yield: 9%)
The titled compounds of Examples 75 and 76 were prepared in the same manner as Example 73 by reacting 3,5-diaminobenzonitrile or 3-(trifluoromethyl)-1,5-phenylenediamine respectively with (S)-1-(2-chloro-8-methylquinazolin-4-yl)-N-ethylpyrrolidin-3-amine prepared in Reference Example 28.
1H NMR (400 MHz, CD3OD) δ 8.00 (d, 1H), 7.83 (s, 1H), 7.47 (d, 1H), 7.31 (s, 1H), 7.08 (t, 1H), 6.56 (s, 1H), 4.22-3.98 (m, 3H), 3.79 (m, 1H), 3.48 (m, 1H), 2.79 (m, 2H), 2.58 (s, 3H), 2.29 (m, 1H), 1.97 (m, 1H), 1.17 (t, 3H); (Yield: 1%)
1H NMR (400 MHz, CD3OD) δ 7.99-7.95 (m, 2H), 7.46 (d, 1H), 7.14 (s, 1H), 7.08 (t, 1H), 6.56 (s, 1H), 4.15-4.11 (m, 2H), 3.99 (m, 1H), 3.77 (m, 1H), 3.48 (m, 1H), 2.76 (m, 2H), 2.57 (s, 3H), 2.28 (m, 1H), 1.95 (m, 1H) 1.18 (t, 3H); (Yield: 3%)
The titled compound was prepared as a pale yellow oil in the same manner as Step 2 of Example 68 by using (R)—N-{1-(2-chloro-8-methylquinazolin-4-yl)piperidin-3-yl}acetamide prepared in Reference Example 29 and 3-(trifluoromethyl)-1,5-phenylenediamine.
1H NMR (400 MHz, CD3OD) δ 7.99 (s, 1H), 7.72 (d, 1H), 7.47 (d, 1H), 7.17 (s, 1H), 7.11 (t, 1H), 6.58 (s, 1H), 4.17 (d, 1H), 4.08 (m, 1H), 3.99 (m, 1H), 3.24 (m, 1H), 3.05 (t, 1H), 2.59 (s, 3H), 2.04 (m, 1H), 1.94 (m, 3H+1H), 1.78 (m, 1H), 1.63 (m, 1H); (Yield: 25%)
t-Butanol (0.5 ml) solution of (R)—N-{1-(2-chloro-8-methylquinazolin-4-yl)piperidin-3-yl}acetamide (20 mg, 0.06 mmol) prepared in Reference Example 29 and 3,5-diaminobenzonitrile (10 mg, 0.08 mmol) was stirred for 1 hour in a microwave (300 W). After cooling the reaction solution to room temperature, the same was filtered. The filtrate was washed with dichloromethane and dried in vacuo to prepare the titled compound (16.7 mg) as a pale yellow solid.
1H NMR (400 MHz, CD3OD) δ 8.22 (m, 1H), 8.01 (s, 1H), 7.72 (d, 1H), 7.40 (t, 1H), 7.22 (brs, NH), 7.11 (s, 1H), 6.74 (s, 1H), 4.74 (m, 1H), 4.55 (m, 1H), 4.04 (m, 1H), 3.57 (m, 1H), 3.41 (m, 1H), 2.56 (s, 3H), 2.10 (m, 2H), 1.94 (s, 3H), 1.87 (m, 1H), 1.74 (m, 1H)
The titled compounds of Examples 79 and 80 were prepared in the same manner as Example 78 by reacting 2,5-diaminobenzonitrile or 2-(trifluoromethyl)-1,4-phenylenediamine respectively with (R)—N-{1-(2-chloro-8-methylquinazolin-4-yl)piperidin-3-yl}acetamide prepared in Reference Example 29.
1H NMR (400 MHz, CD3OD) δ 8.17 (d, 1H), 8.02 (m, 1H), 7.69 (m, 1H), 7.62 (brs, NH), 7.43-7.37 (m, 2H), 6.86 (d, 1H), 4.61 (m, 1H), 4.49 (m, 1H), 3.99 (m, 1H), 3.48 (m, 1H), 3.36 (m, 1H), 2.54 (s, 3H), 2.11 (m, 1H), 1.98 (m, 1H), 1.93 (s, 3H), 1.82-1.69 (m, 2H); (Yield: 63%)
1H NMR (400 MHz, CD3OD) δ 8.17 (m, 1H), 8.02 (m, 1H), 7.73 (brs, NH), 7.67 (d, 1H), 7.35 (m, 2H), 6.88 (d, 1H), 4.60-4.51 (m, 2H), 4.00 (m, 1H), 3.48 (m, 1H), 3.36 (m, 1H), 2.54 (s, 3H), 2.11 (m, 1H), 1.98 (m, 1H), 1.92 (s, 3H), 1.82-1.69 (m, 2H); (Yield: 55%)
The titled compound was prepared as a white solid in the same manner as Step 3 of Reference Example 13 by using 2,4,7-trichloroquinazoline prepared in Reference Example 30 and (S)-3-acetamidopyrrolidine.
1H NMR (400 MHz, CDCl3) δ 7.98 (d, 1H), 7.64 (s, 1H), 7.33 (d, 1H), 6.40 (m, 1H), 4.68 (m, 1H), 4.19-3.88 (m, 4H), 2.32 (m, 1H), 2.15 (m, 1H), 2.04 (s, 3H); (Yield: 96%)
The titled compound was prepared as a white solid in the same manner as Example 30 by using (S)—N-{1-(2,7-dichloroquinazolin-4-yl)pyrrolidin-3-yl}acetamide prepared in Step 1.
1H NMR (400 MHz, CD3OD) δ 8.29 (d, 1H), 7.60 (s, 1H), 7.47 (d, 1H), 7.14 (s, 1H), 7.08 (s, 1H), 6.81 (s, 1H), 4.52 (m, 1H), 4.33-4.18 (m, 3H), 3.96 (m, 1H), 2.36 (m, 1H), 2.16 (m, 1H), 1.96 (s, 3H); (Yield: 69%)
The titled compounds of Examples 82 and 83 were prepared in the same manner as Example 30 by reacting 3,5-diaminobenzonitrile or 3-(trifluoromethyl)-1,5-phenylenediamine respectively with (S)-1-(2,7-dichloroquinazolin-4-yl)-N-methylpyrrolidin-3-amine prepared in Reference Example 31.
1H NMR (400 MHz, CD3OD+DMSO-d6) δ 8.29 (d, 1H), 7.78-7.66 (d, 1H), 7.48 (m, 1H), 7.22-7.08 (d, 2H), 6.39 (s, 1H), 4.40-3.85 (m, 5H), 2.82 (s, 3H), 2.61 (m, 1H), 2.40 (m, 1H); (Yield: 69%)
1H NMR (400 MHz, CD3OD+DMSO-d6) δ 8.29 (d, 1H), 7.81-7.68 (d, 1H), 7.49 (d, 1H), 7.19 (s, 1H), 7.04 (s, 1H), 6.80 (s, 1H), 4.35-4.02 (m, 5H), 2.79 (s, 3H), 2.60 (m, 1H), 2.39 (m, 1H); (Yield: 66%)
The titled compound was prepared as a white solid in the same manner as Step 3 of Reference Example 13 by using 2,4,7-trichloroquinazoline prepared in Reference Example 30 and (3S)-(−)-3-(ethylamino)pyrrolidine.
1H NMR (400 MHz, CD3OD) δ 8.29 (d, 1H), 7.67 (s, 1H), 7.51 (d, 1H), 4.32-4.05 (m, 5H), 3.23 (q, 2H), 2.59 (m, 1H), 2.35 (m, 1H), 1.37 (t, 3H); (Yield: 71%)
The titled compound was prepared as a white solid in the same manner as Example 30 by using (S)-1-(2,7-dichloroquinazolin-4-yl)-N-ethylpyrrolidin-3-amine prepared in Step 1 and 3,5-diaminobenzonitrile.
1H NMR (400 MHz, CD3OD+DMSO-d6) δ 8.25 (d, 1H), 7.78-7.66 (d, 1H), 7.48 (m, 1H), 7.26 (s, 1H), 7.11 (s, 1H), 6.78 (s, 1H), 4.36-4.07 (m, 5H), 3.29 (m, 2H), 2.61 (m, 1H), 2.37 (m, 1H), 1.38 (m, 3H); (Yield: 42%)
The titled compounds of Examples 85 and 86 were prepared in the same manner as Example 30 by reacting 3,5-diaminobenzonitrile or 3-(trifluoromethyl)-1,5-phenylenediamine respectively with (S)—N-{1-(2-chloro-7-fluoroquinazolin-4-yl)pyrrolidin-3-yl}acetamide prepared in Reference Example 32.
1H NMR (400 MHz, CD3OD) δ 8.38 (m, 1H), 7.30 (m, 2H), 7.15 (s, 1H), 7.09 (s, 1H), 6.81 (s, 1H), 4.52 (m, 1H), 4.33-4.18 (m, 3H), 3.94 (m, 1H), 2.35 (m, 1H), 2.15 (m, 1H), 1.96 (s, 3H); (Yield: 71%)
1H NMR (400 MHz, CD3OD) δ 8.37 (m, 1H), 7.30-7.26 (m, 2H), 7.20 (s, 1H), 7.01 (s, 1H), 6.81 (s, 1H), 4.52 (m, 1H), 4.31-4.17 (m, 3H), 3.94 (m, 1H), 2.37 (m, 1H), 2.14 (m, 1H), 1.95 (s, 3H); (Yield: 71%)
n-Butanol (0.3 ml) solution of (S)—N-{1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)pyrrolidin-3-yl}acetamide (30 mg, 0.10 mmol) prepared in Reference Example 34 and 5-amino-2-methylbenzonitrile (16.1 mg, 0.11 mmol) was stirred for 1 hour in a microwave (600 W). After cooling the reaction solution to room temperature, the same was concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=60/1) to prepare the titled compound (13.7 mg) as a white solid.
1H NMR (400 MHz, CDCl3) δ 8.18 (s, 1H), 7.44 (m, 1H), 7.15 (t, 1H), 7.02 (s, 1H), 5.89 (s, 1H), 4.51 (m, 1H), 3.96 (m, 1H), 3.79 (m, 1H), 3.73 (m, 2H), 3.56 (m, 1H), 2.66 (s, 4H), 2.45 (s, 3H), 2.21 (m, 1H), 1.95 (m, 4H), 1.73 (m, 4H)
The titled compounds of Examples 88 to 91 were prepared in the same manner as Example 87 by reacting 3,5-diaminobenzonitrile, 3-(trifluoromethyl)-1,5-phenylenediamine, 4-chloro-1,3-diaminobenzene or 4-methyl-3-(trifluoromethyl)aniline respectively with (S)—N-{1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)pyrrolidin-3-yl}acetamide prepared in Reference Example 34.
1H NMR (400 MHz, CDCl3) δ 7.43 (s, 1H), 7.11 (s, 1H), 6.88 (s, 1H), 6.48 (s, 1H), 5.81 (s, 1H), 4.48 (m, 1H), 4.00-3.59 (m, 4H+2NH), 2.65 (m, 4H), 2.24 (m, 1H), 2.20 (s, 3H), 1.94 (m, 1H), 1.77 (m, 4H); (Yield: 12%)
1H NMR (400 MHz, CDCl3) δ 7.70 (brs, 1H), 7.47 (s, 1H), 6.97 (s, 1H), 6.57-6.49 (brs+s, 2H), 4.52 (m, 1H), 4.00-3.92 (m, 4H), 3.75 (m, 2H), 2.59 (m, 4H), 2.21 (m, 1H), 2.04 (s, 3H), 2.00 (m, 1H), 1.72 (m, 4H); (Yield: 29%)
1H NMR (400 MHz, CDCl3) δ 8.92 (brs, 1H), 7.87 (s, 1H), 7.28 (m, 1H), 7.04 (m, 1H), 6.68 (m, 1H), 4.52 (m, 1H), 4.43 (s, 2H), 4.14 (m, 1H), 3.94 (m, 1H), 3.88 (m, 1H), 3.76 (m, 1H), 2.54 (m, 4H), 2.26 (m, 1H), 2.13 (m, 4H), 1.75 (m, 4H); (Yield: 86%)
1H NMR (400 MHz, CDCl3) δ 8.31 (s, 1H), 7.27 (s, 1H), 7.12 (m, 1H), 6.97 (s, 1H), 5.90 (m, 1H), 4.52 (m, 1H), 3.94 (m, 1H), 3.80-3.73 (m, 2H), 3.55 (m, 1H), 2.64 (m, 4H), 2.40 (s, 3H), 2.19 (m, 1H), 1.93 (m, 4H), 1.72 (m, 4H); (Yield: 33%)
n-Butanol (0.3 ml) solution of (S)—N-{1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)pyrrolidin-3-yl}acetamide (30 mg, 0.10 mmol) prepared in Reference Example 34 and 2-nitro-1,4-phenylenediamine (18.7 mg, 0.11 mmol) was stirred for 1 hour in a microwave (600 W). After cooling the reaction solution to room temperature, the resulting solid was washed with dichloromethane, filtered and dried in vacuo to prepare the titled compound (40.5 mg) as a red solid.
1H NMR (400 MHz, CD3OD) δ 8.50 (s, 1H), 7.38 (d, 1H), 6.98 (d, 1H), 4.39 (m, 1H), 4.11-3.96 (m, 3H), 3.72 (m, 1H), 2.79 (m, 2H), 2.66 (m, 2H), 2.24 (m, 1H), 1.95 (m, 3H+1H), 1.95-1.84 (m, 4H)
The titled compound was prepared as a white solid in the same manner as Example 92 by using (S)—N-{1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)pyrrolidin-3-yl}acetamide prepared in Reference Example 34 and 5-chloro-1,3-phenylenediamine.
1H NMR (400 MHz, CD3OD) δ 6.89 (s, 1H), 6.74 (s, 1H), 6.48 (s, 1H), 4.41 (m, 1H), 4.11-3.96 (m, 3H), 3.74 (m, 1H), 2.79 (m, 2H), 2.66 (m, 2H), 2.29 (m, 1H), 1.96 (m, 1H+3H), 1.83 (m, 4H); (Yield: 85%)
The titled compounds of Examples 94 and 95 were prepared in the same manner as Example 31 by reacting (S)—N-[1-{2-(3-amino-5-cyanophenylamino)-5,6,7,8-tetrahydroquinazolin-4-yl}pyrrolidin-3-yl]acetamide prepared in Example 88 or (S)—N-(1-[2-{3-amino-5-(trifluoromethyl)phenylamino}-5,6,7,8-tetrahydroquinazolin-4-yl]pyrrolidin-3-yl)acetamide prepared in Example 89.
1H NMR (400 MHz, CD3OD) δ 7.94 (s, 2H), 7.40 (m, 1H), 4.41 (m, 1H), 4.11-3.75 (m, 4H), 2.85 (m, 2H), 2.73 (m, 2H), 2.26 (m, 1H), 2.01 (m, 4H), 1.86 (m, 4H); (Yield: 90%)
1H NMR (400 MHz, CD3OD) δ 8.12 (m, 1H), 7.87 (s, 1H), 7.40 (s, 1H), 4.41-3.73 (m, 5H), 2.85 (m, 2H), 2.74 (m, 2H), 2.25 (m, 1H), 2.01 (m, 4H), 1.82 (m, 4H); (Yield: 90%)
n-Butanol (1 ml) solution of (S)-tert-butyl 1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)pyrrolidin-3-ylcarbamate (40 mg, 0.11 mmol) prepared in Reference Example 35 and 3,5-diaminobenzonitrile (18.1 mg, 0.14 mmol) was stirred for 1.5 hours in a microwave (450 W). After cooling the reaction solution to room temperature, the same was concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=60/1), dissolved in ethyl acetate (1 ml), and then hydrochloric acid gas was added thereto. The resulting white solid was filtered to prepare the titled compound (2.5 mg).
1H NMR (400 MHz, CD3OD) δ 7.46-7.17 (m, 2H), 6.95-6.85 (m, 1H), 4.15-4.03 (m, 5H), 2.84-2.73 (m, 4H), 2.46 (m, 1H), 2.22 (m, 1H), 1.79 (m, 4H)
The titled compounds of Examples 97 and 98 were prepared in the same manner as Example 96 by reacting 3-(trifluoromethyl)-1,5-phenylenediamine or 2-(trifluoromethyl)-1,4-phenylenediamine respectively with (S)-tert-butyl 1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)pyrrolidin-3-ylcarbamate prepared in Reference Example 35.
1H NMR (400 MHz, CD3OD) δ 7.91-7.72 (m, 1H), 7.25 (m, 1H), 6.81 (m, 1H), 4.15-4.05 (m, 5H), 2.86-2.76 (m, 4H), 2.45 (m, 1H), 2.21 (m, 1H), 1.79 (m, 4H); (Yield: 24%)
1H NMR (400 MHz, CD3OD) δ 7.68 (m, 1H), 7.43 (m, 1H), 6.9 (m, 1H), 4.09-4.00 (m, 5H), 2.82-2.70 (m, 4H), 2.42 (m, 1H), 2.18 (m, 1H), 1.77 (m, 4H); (Yield: 21%)
A mixture of (S)-tert-butyl 1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)pyrrolidin-3-yl(methyl)carbamate (50 mg, 0.14 mmol) prepared in Reference Example 36, 3,5-diaminobenzonitrile (21.8 mg, 0.16 mmol), palladium acetate (0.6 mg, 0.003 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthine (2.4 mg, 0.005 mmol), cesium carbonate (90.0 mg, 0.24 mmol) and anhydrous 1,4-dioxane (0.7 ml) was stirred at 130° C. for 3 hours. After cooling the reaction solution to room temperature, the same was filtered by celite and the filtrate was concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=100/1), dissolved in ethyl acetate (1 ml), and then hydrochloric acid gas was added thereto. The resulting white solid was filtered to prepare the titled compound (10.6 mg).
1H NMR (400 MHz, CD3OD) δ 7.85 (s, 1H), 7.69 (m, 1H), 7.27 (m, 1H), 4.25-3.96 (m, 5H), 2.86-2.82 (m, 5H), 2.75 (m, 2H), 2.49 (m, 1H), 2.31 (m, 1H), 1.82 (m, 4H)
The titled compound was prepared as a white solid in the same manner as Example 99 by using (S)-tert-butyl 1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)pyrrolidin-3-yl(methyl)carbamate prepared in Reference Example 36 and 3-(trifluoromethyl)-1,5-phenylenediamine.
1H NMR (400 MHz, CD3OD) δ 7.93 (s, 1H), 7.80 (m, 1H), 7.31 (m, 1H), 4.11-3.97 (m, 5H), 2.87-2.80 (m, 5H), 2.76 (m, 2H), 2.48 (m, 1H), 2.32 (m, 1H), 1.60 (m, 4H); (Yield: 30%)
The titled compound was prepared as a white solid in the same manner as Example 96 by using (S)-tert-butyl 1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)pyrrolidin-3-yl(methyl)carbamate prepared in Reference Example 36 and 2-(trifluoromethyl)-1,4-phenylenediamine.
1H NMR (400 MHz, CD3OD) δ 7.75 (s, 1H), 7.48 (s, 1H), 7.07 (m, 1H), 4.14-3.92 (m, 5H), 2.83-2.78 (m, 5H), 2.70 (m, 2H), 2.44 (m, 1H), 2.26 (m, 1H), 1.84 (m, 4H); (Yield: 32%)
The titled compounds of Examples 102 to 105 were prepared in the same manner as Example 96 by reacting 3-(trifluoromethyl)-1,5-phenylenediamine, 2-(trifluoromethyl)-1,4-phenylenediamine, 4-chloro-1,3-diaminobenzene or 3,5-diaminobenzonitrile respectively with (S)-tert-butyl 1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)pyrrolidin-3-yl(ethyl)carbamate prepared in Reference Example 37.
1H NMR (400 MHz, CD3OD) δ 7.92 (m, 2H), 7.37 (m, 1H), 4.23-4.00 (m, 5H), 3.18 (m, 2H), 2.87-2.76 (m, 4H), 2.48 (m, 1H), 2.30 (m, 1H), 1.86 (m, 4H), 1.38 (m, 3H); (Yield: 21%)
1H NMR (400 MHz, CD3OD) δ 7.93 (m, 1H), 7.67 (m, 1H), 7.30 (m, 1H), 4.17-3.99 (m, 5H), 3.16 (m, 2H), 2.85-2.72 (m, 4H), 2.46 (m, 1H), 2.28 (m, 1H), 1.82 (m, 4H), 1.38 (m, 3H); (Yield: 47%)
1H NMR (400 MHz, CD3OD) δ 7.62-7.20 (m, 3H), 4.22-3.98 (m, 5H), 3.18 (m, 2H), 2.84-2.72 (m, 4H), 2.46 (m, 1H), 2.28 (m, 1H), 1.81 (m, 4H), 1.38 (m, 3H); (Yield: 51%)
1H NMR (400 MHz, CD3OD) δ 7.76 (s, 1H), 7.59 (s, 1H), 7.19 (m, 1H), 4.26-4.00 (m, 5H), 3.20 (m, 2H), 2.86-2.75 (m, 4H), 2.49 (m, 1H), 2.29 (m, 1H), 1.86 (m, 4H), 1.38 (m, 3H); (Yield: 31%)
Propionaldehyde (19.6 μl, 0.273 mmol) was added into methanol (1.5 ml) solution of (S)—N1-{4-(3-aminopyrrolidin-1-yl)-5,6,7,8-tetrahydroquinazolin-2-yl}-5-(trifluoromethyl)benzene-1,3-diamine (107 mg, 0.273 mmol) prepared by treating (S)—N1-{4-(3-aminopyrrolidin-1-yl)-5,6,7,8-tetrahydroquinazolin-2-yl}-5-(trifluoromethyl)benzene-1,3-diamine dihydrochloride prepared in Example 97 with 2.0 N sodium hydroxide aqueous solution, and then they were stirred at room temperature for 1 hour and sodium triacetoxyborohydride (115.6 mg, 0.545 mmol) was added thereto. The reaction solution was stirred at room temperature overnight, and then water was added to terminate the reaction. The reaction mixture was extracted by adding chloroform, and the extract was washed with saturated sodium bicarbonate aqueous solution, dried by anhydrous magnesium sulfate and filtered. The solution was concentrated. The resulting residue was purified with silica gel column chromatography (ethyl acetate/methanol=100/1) to prepare the titled compound (7.5 mg) as a colorless oil.
1H NMR (400 MHz, CD3OD) δ 7.57 (s, 1H), 7.03 (s, 1H), 6.52 (s, 1H), 3.89 (m, 2H), 3.72 (m, 1H), 3.51 (m, 1H), 3.33 (m, 1H), 2.73 (m, 2H), 2.60 (m, 4H), 2.18 (m, 1H), 1.80 (m, 4H), 1.65 (m, 1H), 1.55 (m, 2H), 0.95 (m, 3H)
The titled compound was prepared as a white solid in the same manner as Reference Example 36 by using 2,4-dichloro-5,6,7,8-tetrahydroquinazoline prepared in Reference Example 33 and (R)-(−)-3-aminopiperidine dihydrochloride. This compound was used in the subsequent reaction without further purification.
The titled compound (441 mg) was prepared as a pale yellow oil in the same manner as Step 2 of Example 59 by using (R)-tert-butyl 1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)piperidin-3-ylcarbamate prepared in Step 1 and 3-(trifluoromethyl)-1,5-phenylenediamine.
1H NMR (400 MHz, CDCl3) δ 7.55 (s, 1H), 7.08 (s, 1H), 6.97 (s, 1H), 6.50 (s, 1H), 4.96 (m, 1H), 3.82 (s, 2H), 3.56 (m, 1H), 3.37-3.26 (m, 3H), 2.71 (m, 2H), 2.50 (m, 2H), 2.02 (m, 1H), 1.84 (m, 4H), 1.70-1.42 (m, 4H), 1.42 (s, 9H); (Yield: 40%)
The titled compound was prepared as a white solid in the same manner as Example 31 by using (R)-tert-butyl 1-[2-{3-amino-5-(trifluoromethyl)phenylamino}-5,6,7,8-tetrahydroquinazolin-4-yl]piperidin-3-ylcarbamate prepared in Step 2.
1H NMR (400 MHz, CD3OD) δ 8.02 (s, 1H), 7.90 (s, 1H), 4.42 (m, 1H), 4.12 (m, 1H), 3.61-3.31 (m, 3H), 2.84 (m, 2H), 2.70 (m, 2H), 2.22 (m, 1H), 1.99 (m, 3H), 1.82 (m, 4H); (Yield: 90%)
The titled compounds of Examples 108 to 117 were prepared in the same manner as Example 87 by reacting 3-aminobenzonitrile, 5-amino-2-methylbenzonitrile, 5-amino-2-fluorobenzonitrile, 3,5-diaminobenzonitrile, 3-(trifluoromethyl)-1,5-phenylenediamine, 2-(trifluoromethyl)-1,4-phenylenediamine, 4-fluoro-3-trifluoromethylphenylamine, 2-nitro-1,4-phenylenediamine, 4-chloro-1,3-diaminobenzene or 5-chloro-1,3-diaminobenzene respectively with (R)—N-{1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)piperidin-3-yl}acetamide prepared in Reference Example 38.
1H NMR (400 MHz, CDCl3) δ 8.37 (s, 1H), 7.50 (m, 1H), 7.36 (m, 1H), 7.20 (m, 1H), 6.97 (s, 1H), 5.93 (m, 1H), 4.08 (m, 1H), 3.67 (m, 1H), 3.45 (m, 1H), 3.16 (m, 2H), 2.73 (m, 2H), 2.51 (m, 2H), 1.90 (s, 3H), 1.85-1.74 (m, 8H); (Yield: 30%)
1H NMR (400 MHz, CDCl3) δ 8.28 (s, 1H), 7.38 (d, 1H), 7.17 (d, 1H), 6.89 (s, 1H), 6.01 (m, 1H), 4.07 (m, 1H), 3.67 (d, 1H), 3.43 (m, 1H), 3.17 (m, 2H), 2.71 (m, 2H), 2.49 (m, 2H+3H), 1.94 (s, 3H), 1.94-1.63 (m, 8H); (Yield: 28%)
1H NMR (400 MHz, CDCl3) δ 8.33 (m, 1H), 7.47 (m, 1H), 7.09 (m, 1H), 6.96 (s, 1H), 5.85 (m, 1H), 4.08 (m, 1H), 3.72 (m, 1H), 3.46 (m, 1H), 3.13-3.05 (m, 2H), 2.72 (m, 2H), 2.51 (m, 2H), 1.95 (s, 3H), 1.84-1.58 (m, 8H); (Yield: 29%)
1H NMR (400 MHz, CDCl3) δ 7.45 (s, 1H), 7.06 (s, 1H), 6.88 (s, 1H), 6.50 (s, 1H), 6.03 (m, 1H), 4.07 (m, 1H), 3.91 (s, 2H), 3.73 (m, 1H), 3.46 (m, 1H), 3.18 (m, 2H), 2.71 (m, 2H), 2.50 (m, 2H), 1.91 (s, 3H), 1.85-1.64 (m, 8H); (Yield: 23%)
1H NMR (400 MHz, CDCl3) δ 7.52 (s, 1H), 6.94 (s, 1H), 6.88 (s, 1H), 6.51 (s, 1H), 6.21 (m, 1H), 4.05 (m, 1H), 3.86 (s, 2H), 3.62 (m, 1H), 3.40-3.29 (m, 3H), 2.72 (m, 2H), 2.49 (m, 2H), 1.90-1.68 (m, 3H+8H); (Yield: 30%)
1H NMR (400 MHz, CDCl3) δ 7.99 (s, 1H), 7.19 (d, 1H), 6.80 (s, 1H), 6.68 (d, 1H), 6.43 (m, 1H), 4.02 (m, 1H+2H), 3.48 (m, 2H), 3.33 (m, 2H), 2.68 (m, 2H), 2.47 (m, 2H), 1.89-1.60 (m, 3H+8H); (Yield: 37%)
1H NMR (400 MHz, CDCl3) δ 8.31 (m, 1H), 7.38 (m, 1H), 7.11 (m, 1H), 6.89 (s, 1H), 5.94 (m, 1H), 4.08 (m, 1H), 3.61 (m, 1H), 3.49 (m, 1H), 3.18 (m, 2H), 2.71 (m, 2H), 2.50 (m, 2H), 1.89 (s, 3H), 1.78-1.68 (m, 8H); (Yield: 29%)
1H NMR (400 MHz, CDCl3) δ 8.91 (s, 1H), 7.23 (m, 1H), 6.74 (d, 2H), 6.14 (m, 1H), 5.89 (s, 2H), 4.07 (m, 1H), 3.72 (m, 1H), 3.32 (m, 1H), 3.29 (m, 1H), 3.18 (m, 1H), 2.68 (m, 2H), 2.48 (m, 2H), 1.93 (s, 3H), 1.89-1.63 (m, 8H); (Yield: 29%)
1H NMR (400 MHz, CDCl3) δ 7.24 (d, 1H), 7.09 (d, 1H), 6.83 (s, 1H), 6.73 (d, 1H), 6.39 (m, 1H), 4.08 (m, 1H+2H), 3.62 (m, 1H), 3.40-3.34 (m, 3H), 2.70 (t, 2H), 2.49 (m, 2H), 1.86-1.63 (m, 3H+8H); (Yield: 35%)
1H NMR (400 MHz, CDCl3) δ 7.12 (s, 1H), 6.95 (s, 1H), 6.76 (s, 1H), 6.36 (m, 1H), 6.29 (s, 1H), 4.07 (m, 1H), 3.75 (s, 2H), 3.58 (m, 1H), 3.43-3.36 (m, 3H), 2.70 (t, 2H), 2.49 (m, 2H), 1.88-1.74 (m, 3H+8H); (Yield: 30%)
The titled compound was prepared as a white solid in the same manner as Example 31 by using (R)—N-(1-[2-{4-amino-3-(trifluoromethyl)phenylamino}-5,6,7,8-tetrahydroquinazolin-4-yl]piperidin-3-yl)acetamide prepared in Example 113.
1H NMR (400 MHz, CD3OD) δ 7.77 (s, 1H), 7.48 (m, 1H), 7.10 (m, 1H), 4.31 (m, 1H), 4.11 (m, 1H), 3.82 (m, 1H), 3.22 (m, 2H), 2.74 (m, 2H), 2.59 (m, 2H), 1.87 (s, 3H), 1.87-1.61 (m, 8H); (Yield: 95%)
The titled compound was prepared as a white solid in the same manner as Example 99 by using (R)—N-{1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)piperidin-3-yl}acetamide prepared in Reference Example 38.
1H NMR (400 MHz, CD3OD) δ 7.97 (s, 1H), 7.90 (s, 1H), 7.46 (s, 1H), 4.57 (m, 1H), 4.16 (m, 1H), 3.86 (m, 1H), 3.43 (m, 1H), 3.22 (m, 1H), 2.79 (m, 2H), 2.65 (m, 2H), 2.05-1.68 (m, 3H+8H); (Yield: 29%)
Diisopropylethylamine (3.4 ml, 19.7 mmol) was added into chloroform (25 ml) solution of 2,4-dichloro-5,6,7,8-tetrahydroquinazoline (1 g, 4.92 mmol) prepared in Reference Example 33 and (S)-(+)-pyrrolidine-3-carboxylic acid (0.62 g, 5.42 mmol), and they were stirred at 60° C. for 2 days. After cooling the reaction solution to room temperature, methylamine hydrochloride (0.33 g, 4.92 mmol), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (0.94 g, 4.92 mmol) and 1-hydroxybenzotriazole hydrate (0.67 g, 4.92 mmol) were added thereto, and they were stirred at room temperature overnight. The reaction solution was diluted with dichloromethane, washed with water, dried with anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting residue was crystallized by using ether/ethyl acetate to prepare the titled compound (810 mg) as a pale yellow solid.
1H NMR (400 MHz, CDCl3) δ 5.65 (s, 1H), 3.91-3.68 (m, 4H), 2.89 (s, 3H), 2.72 (m, 4H), 2.16 (m, 2H), 1.78-1.43 (m, 4H)
The titled compound was prepared as a pale yellow oil in the same manner as Example 87 by using (S)-1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)-N-methylpyrrolidine-3-carboxamide prepared in Step 1 and 3,5-diaminobenzonitrile.
1H NMR (400 MHz, CDCl3) δ 7.50 (m, 1H+1H), 7.04 (s, 1H), 6.47 (s, 1H), 5.99 (s, 1H), 3.94-3.70 (m, 2H+4H), 2.96 (m, 1H), 2.85 (s, 3H), 2.65 (m, 4H), 2.22 (m, 2H), 1.73-1.60 (m, 4H); (Yield: 20%)
The titled compounds of Examples 121 to 126 were prepared in the same manner as Example 87 by reacting 5-amino-2-methylbenzonitrile, 5-amino-2-fluorobenzonitrile, 3-(trifluoromethyl)-1,5-phenylenediamine, 2-(trifluoromethyl)-1,4-phenylenediamine, 4-fluoro-3-trifluoromethylphenylamine or 4-chloro-1,3-diaminobenzene respectively with (S)-1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)-N-methylpiperidine-3-carboxamide prepared in Reference Example 39.
1H NMR (400 MHz, CDCl3) δ 8.07 (s, 1H), 7.81 (s, 1H), 7.53 (d, 1H), 7.18 (d, 1H), 6.08 (s, 1H), 4.06 (d, 1H), 3.85 (d, 1H), 3.24 (t, 1H), 3.07 (t, 1H), 2.80 (s, 3H), 2.70 (m, 2H), 2.47 (m, 6H), 1.99 (m, 1H), 1.83 (m, 4H), 1.68 (m, 3H); (Yield: 50%)
1H NMR (400 MHz, CDCl3) δ 8.17 (s, 1H), 7.60 (m, 1H), 7.10 (m, 1H), 6.94 (s, 1H), 5.82 (m, 1H), 3.96 (d, 1H), 3.74 (d, 1H), 3.16 (t, 1H), 2.99 (t, 1H), 2.82 (s, 3H), 2.72 (m, 2H), 2.49 (m, 3H), 2.01 (m, 1H), 1.83-1.71 (m, 7H); (Yield: 17%)
1H NMR (400 MHz, CDCl3) δ 7.29 (m, 1H), 7.17 (s, 1H), 6.90 (s, 1H), 6.49 (s, 1H), 5.87 (s, 1H), 4.08 (m, 1H), 3.99 (s, 2H), 3.74 (d, 1H), 3.20 (m, 1H), 2.95 (m, 1H), 2.77 (s, 3H), 2.70 (m, 2H), 2.56 (m, 1H), 2.48 (m, 2H), 1.97 (m, 1H), 1.78-1.61 (m, 7H); (Yield: 11%)
1H NMR (400 MHz, CDCl3) δ 7.86 (s, 1H), 7.31 (d, 1H), 6.71 (m, 2H), 5.98 (s, 1H), 3.97 (s, 2H), 3.84 (d, 1H), 3.64 (d, 1H), 3.27 (t, 1H), 3.07 (t, 1H), 2.73 (m, 5H), 2.47 (m, 3H), 1.89-1.83 (m, 4H), 1.69-1.60 (m, 4H); (Yield: 14%)
1H NMR (400 MHz, CDCl3) δ 8.17 (s, 1H), 7.51 (m, 1H), 7.09 (m, 1H), 6.92 (m, 1H), 5.82 (m, 1H), 3.92 (m, 1H), 3.71 (m, 1H), 3.19 (m, 1H), 3.01 (m, 1H), 2.80-2.71 (m, 5H), 2.49 (m, 3H), 1.96-1.71 (m, 8H); (Yield: 11%)
1H NMR (400 MHz, CDCl3) δ 7.45 (s, 1H), 7.07 (m, 2H), 6.61 (m, 1H), 5.93 (m, 1H), 4.25 (s, 2H), 4.14 (d, 1H), 3.76 (d, 1H), 3.17 (t, 1H), 3.01 (t, 1H), 2.79 (s, 3H), 2.69-2.46 (m, 3H), 2.46 (m, 2H), 1.95-1.53 (m, 8H); (Yield: 20%)
The titled compounds of Examples 127 to 129 were prepared in the same manner as Example 92 by reacting 3,5-diaminobenzonitrile, 2-nitro-1,4-phenylenediamine or 5-chloro-1,3-diaminobenzene respectively with (S)-1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)-N-methylpiperidine-3-carboxamide prepared in Reference Example 39.
1H NMR (400 MHz, CD3OD) δ 7.84 (s, 1H), 7.08 (s, 1H), 7.02 (s, 1H), 6.75 (s, 1H), 4.43 (d, 1H), 4.25 (d, 1H), 3.37 (m, 2H), 2.72 (m, 5H), 2.53 (m, 3H), 2.03 (m, 1H), 1.92-1.82 (m, 5H), 1.68-1.62 (m, 2H); (Yield: 63%)
1H NMR (400 MHz, CD3OD) δ 8.29 (s, 1H), 7.78 (m, 1H), 7.37 (d, 1H), 7.00 (d, 1H), 4.39 (d, 1H), 4.21 (d, 1H), 3.30 (m, 2H), 2.70 (m, 5H), 2.57 (m, 2H), 2.47 (m, 1H), 1.99-1.57 (m, 8H); (Yield: 72%)
1H NMR (400 MHz, CD3OD) δ 7.84 (s, 1H), 6.83 (s, 1H), 6.68 (s, 1H), 6.50 (s, 1H), 4.43 (d, 1H), 4.24 (d, 1H), 3.30 (m, 2H), 2.72 (m, 5H), 2.53 (m, 3H), 2.00-1.64 (m, 8H); (Yield: 65%)
The titled compound was prepared as a white solid in the same manner as Example 99 by using (S)-1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)-N-methylpiperidine-3-carboxamide prepared in Reference Example 39 and 3-(trifluoromethyl)-1,5-phenylenediamine.
1H NMR (400 MHz, CD3OD) δ 8.07 (s, 1H), 7.97 (s, 1H), 7.45 (s, 1H), 4.49 (m, 1H), 4.25 (m, 1H), 3.40 (m, 2H), 2.80 (m, 2H), 2.62 (s, 3H), 2.58 (m, 3H), 2.04-1.67 (m, 8H); (Yield: 52%)
A mixture of (S)—N-{1-(2-chloroquinazolin-4-yl)pyrrolidin-3-yl}acetamide (20 mg, 0.07 mmol) prepared in Reference Example 40 and 5-(trifluoromethyl)-1,3-phenylenediamine (15 mg, 0.08 mmol) was stirred for 40 minutes in a microwave (600 W). After cooling to room temperature, the resulting product was purified with silica gel column chromatography (dichloromethane/methanol=20/1) to prepare the titled compound (10 mg) as a pale brown solid.
1H NMR (400 MHz, CD3OD) δ 8.15 (d, 1H), 7.62 (t, 1H), 7.55-7.45 (m, 2H), 7.23 (t, 1H), 7.17 (s, 1H), 6.61 (s, 1H), 4.55-4.45 (m, 1H), 4.30-4.00 (m, 3H), 3.86 (dd, 1H), 2.35-2.20 (m, 1H), 2.15-2.05 (m, 1H), 1.95 (s, 3H)
The titled compounds of Examples 132 to 134 were prepared in the same manner as Example 131 by reacting 4-fluoro-1,3-diaminobenzene, 4-chloro-1,3-diaminobenzene or 3,5-diaminobenzonitrile respectively with (S)—N-{1-(2-chloroquinazolin-4-yl)pyrrolidin-3-yl}acetamide prepared in Reference Example 40.
1H NMR (400 MHz, CD3OD) δ 8.16 (d, 1H), 7.64 (t, 1H), 7.47 (dd, 1H), 7.26 (t, 1H), 7.18 (dd, 1H), 6.95-6.75 (m, 2H), 4.48 (t, 1H), 4.30-4.00 (m, 3H), 3.88 (dd, 1H), 2.35-2.25 (m, 1H), 2.15-2.00 (m, 1H), 1.95 (s, 3H); (Yield: 21%)
1H NMR (400 MHz, CD3OD) δ 8.14 (d, 1H), 7.62 (t, 1H), 7.47 (d, 1H), 7.28 (d, 1H), 7.24 (t, 1H), 7.11 (d, 1H), 6.92 (dd, 1H), 4.48 (t, 1H), 4.30-4.00 (m, 3H), 3.86 (dd, 1H), 2.35-2.25 (m, 1H), 2.15-2.00 (m, 1H), 1.95 (s, 3H); (Yield: 15%)
1H NMR (400 MHz, CD3OD) δ 8.16 (d, 1H), 7.63 (t, 1H), 7.51 (d, 1H), 7.45 (s, 1H), 7.31 (s, 1H), 7.25 (t, 1H), 6.60 (s, 1H), 4.50 (t, 1H), 4.30-4.00 (m, 3H), 3.87 (dd, 1H), 2.35-2.25 (m, 1H), 2.15-2.00 (m, 1H), 1.97 (s, 3H); (Yield: 20%)
A mixture of (S)—N-{1-(2-chloroquinazolin-4-yl)pyrrolidin-3-yl}acetamide (20 mg, 0.07 mmol) prepared in Reference Example 40, 4-nitro-1,3-phenylenediamine (11.8 mg, 0.08 mmol), palladium acetate (0.77 mg, 0.003 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthine (4.0 mg, 0.01 mmol), cesium carbonate (44.5 mg, 0.14 mmol) and anhydrous 1,4-dioxane (1 ml) was stirred for 1 hour in a microwave (600 W). After cooling the reaction solution to room temperature, the same was filtered by celite, and then the filtrate was concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=20/1) to prepare the titled compound (5 mg) as a pale yellow solid.
1H NMR (400 MHz, CD3OD) δ 8.16 (d, 1H), 7.96 (d, 1H), 7.74 (s, 1H), 7.70-7.55 (m, 2H), 7.25 (t, 1H), 6.83 (d, 1H), 4.50-4.40 (m, 1H), 4.30-4.00 (m, 3H), 3.85 (d, 1H), 2.40-2.25 (m, 1H), 2.15-2.00 (m, 1H), 1.96 (s, 3H)
The titled compound was prepared as a pale yellow solid in the same manner as Example 135 by using (S)—N-{1-(2-chloroquinazolin-4-yl)pyrrolidin-3-yl}acetamide prepared in Reference Example 40 and 2-nitro-1,4-phenylenediamine.
1H NMR (400 MHz, CD3OD) δ 8.71 (s, 1H), 8.16 (d, 1H), 7.63 (t, 1H), 7.55-7.45 (m, 2H), 7.24 (t, 1H), 6.95 (d, 1H), 4.55-4.45 (m, 1H), 4.35-4.00 (m, 3H), 3.89 (d, 1H), 2.35-2.25 (m, 1H), 2.15-2.05 (m, 1H), 1.96 (s, 3H); (Yield: 11%)
A mixture of (S)-1-(2-chloroquinazolin-4-yl)-N-methylpyrrolidin-3-amine (25 mg, 0.1 mmol) prepared in Reference Example 41, 5-(trifluoromethyl)-1,3-phenylenediamine (21.3 mg, 0.12 mmol), palladium acetate (0.22 mg, 0.001 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthine (1.7 mg, 0.003 mmol), cesium carbonate (81.5 mg, 0.25 mmol) and anhydrous 1,4-dioxane (1 ml) was stirred for 1 hour in a microwave (600 W). After cooling the reaction solution to room temperature, the same was filtered by using celite and the filtrate was concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=20/1) to prepare the titled compound (5 mg) as a pale yellow solid.
1H NMR (400 MHz, CD3OD) δ 8.14 (d, 1H), 7.65-7.55 (m, 2H), 7.45 (d, 1H), 7.20-7.10 (m, 2H), 6.57 (s, 1H), 4.20-4.05 (m, 2H), 4.05-3.95 (m, 1H), 3.85-3.75 (m, 1H), 3.45-3.40 (m, 1H), 2.47 (s, 3H), 2.35-2.25 (m, 1H), 2.05-1.95 (m, 1H)
The titled compounds of Examples 138 and 139 were prepared in the same manner as Example 137 by reacting 4-chloro-1,3-diaminobenzene or 3,5-diaminobenzonitrile respectively with (S)-1-(2-chloroquinazolin-4-yl)-N-methylpyrrolidin-3-amine prepared in Reference Example 41.
1H NMR (400 MHz, CD3OD) δ 8.12 (d, 1H), 7.55 (d, 1H), 7.45 (d, 1H), 7.36 (d, 1H), 7.16 (t, 1H), 7.08 (d, 1H), 6.95 (d, 1H), 4.20-4.05 (m, 2H), 4.05-3.95 (m, 1H), 3.85-3.75 (m, 1H), 3.45-3.35 (m, 1H), 2.46 (s, 3H), 2.35-2.25 (m, 1H), 2.05-1.90 (m, 1H); (Yield: 22%)
1H NMR (400 MHz, CD3OD) δ 8.11 (d, 1H), 7.60-7.40 (m, 3H), 7.29 (d, 1H), 7.17 (t, 1H), 6.55 (d, 1H), 4.20-4.00 (m, 2H), 4.00-3.90 (m, 1H), 3.85-3.75 (m, 1H), 3.45-3.35 (m, 1H), 2.46 (s, 3H), 2.30-2.20 (m, 1H), 2.00-1.90 (m, 1H); (Yield: 20%)
The titled compounds of Examples 140 to 142 were prepared in the same manner as Example 137 by reacting 5-(trifluoromethyl)-1,3-phenylenediamine, 3,5-diaminobenzonitrile or 4-chloro-1,3-diaminobenzene respectively with (S)-1-(2-chloroquinazolin-4-yl)-N-ethylpyrrolidin-3-amine prepared in Reference Example 42.
1H NMR (400 MHz, CD3OD) δ 8.15 (d, 1H), 7.65-7.50 (m, 2H), 7.46 (d, 1H), 7.20-7.15 (m, 2H), 6.57 (s, 1H), 4.20-4.05 (m, 2H), 4.02 (q, 1H), 3.85-3.75 (m, 1H), 3.60-3.50 (m, 1H), 2.78 (q, 2H), 2.35-2.25 (m, 1H), 2.10-1.90 (m, 1H), 1.19 (t, 3H); (Yield: 25%)
1H NMR (400 MHz, CD3OD) δ 8.14 (d, 1H), 7.65-7.45 (m, 3H), 7.29 (s, 1H), 7.18 (t, 1H), 6.56 (s, 1H), 4.20-4.05 (m, 2H), 3.99 (q, 1H), 3.80-3.70 (m, 1H), 3.51 (t, 1H), 2.76 (q, 2H), 2.35-2.25 (m, 1H), 2.00-1.90 (m, 1H), 1.18 (t, 3H); (Yield: 21%)
1H NMR (400 MHz, CD3OD) δ 8.14 (d, 1H), 7.70-7.00 (m, 6H), 4.25-4.10 (m, 2H), 4.10-4.00 (m, 1H), 3.90-3.80 (m, 1H), 3.65-3.55 (m, 1H), 2.85-2.70 (m, 2H), 2.35-2.25 (m, 1H), 2.10-1.95 (m, 1H), 1.14 (t, 3H); (Yield: 12%)
The titled compounds of Examples 143 and 144 were prepared in the same manner as Example 137 by reacting 5-(trifluoromethyl)-1,3-phenylenediamine or 3,5-diaminobenzonitrile respectively with (R)—N-{1-(2-chloroquinazolin-4-yl)piperidin-3-yl}acetamide prepared in Reference Example 43.
1H NMR (400 MHz, CD3OD) δ 7.95 (d, 1H), 7.80 (d, 1H), 7.70-7.45 (m, 3H), 7.35-7.15 (m, 2H), 4.35-4.20 (m, 1H), 4.95-3.35 (m, 3H), 3.25-3.05 (m, 1H), 2.10-1.95 (m, 2H), 1.92 (s, 3H), 1.91-1.55 (m, 2H); (Yield: 21%)
1H NMR (400 MHz, CD3OD) δ 7.92 (d, 1H), 7.70-7.55 (m, 2H), 7.50 (d, 1H), 7.35 (d, 1H), 7.24 (s, 1H), 6.58 (d, 1H), 4.25 (d, 1H), 4.15-4.00 (m, 2H), 3.59 (d, 1H), 3.14 (t, 1H), 2.15-1.95 (m, 2H), 1.93 (s, 3H), 1.90-1.75 (m, 1H), 1.75-1.55 (m, 1H); (Yield: 18%)
The titled compounds of Examples 145 to 154 were prepared in the same manner as Example 137 by reacting 5-(trifluoromethyl)-1,3-phenylenediamine, 4-chloro-1,3-diaminobenzene, 3,5-diaminobenzonitrile, 2,5-diaminobenzonitrile, 3-methoxy-4-methylaniline, 4-methyl-3-(trifluoromethyl)aniline, 5-amino-2-methylpyridine, 4-amino-2-fluoropyridine, 6-amino-2-methylpyridine-3-carbonitrile or 6-amino-3-picholine respectively with (S)—N-{1-(2-chloro-7-methoxyquinazolin-4-yl)pyrrolidine-3-yl}acetamide prepared in Reference Example 44.
1H NMR (400 MHz, CD3OD) δ 7.98 (d, 1H), 7.50 (s, 1H), 7.19 (s, 1H), 6.83 (s, 1H), 6.77 (d, 1H), 6.58 (s, 1H), 4.47 (t, 1H), 4.20-3.90 (m, 3H), 3.87 (s, 3H), 3.77 (dd, 1H), 2.30-2.20 (m, 1H), 2.15-2.00 (m, 1H), 1.95 (s, 3H); (Yield: 23%)
1H NMR (400 MHz, CD3OD) δ 7.94 (d, 1H), 7.31 (s, 1H), 7.07 (d, 1H), 6.91 (d, 1H), 6.82 (s, 1H), 6.75 (d, 1H), 4.43 (t, 1H), 4.20-3.88 (m, 3H), 3.86 (s, 3H), 3.85-3.70 (m, 1H), 2.35-2.20 (m, 1H), 2.10-2.00 (m, 1H), 1.95 (s, 3H); (Yield: 15%)
1H NMR (400 MHz, CD3OD) δ 7.97 (d, 1H), 7.43 (s, 1H), 7.28 (s, 1H), 6.86 (s, 1H), 6.78 (dd, 1H), 6.56 (s, 1H), 4.46 (t, 1H), 4.20-3.90 (m, 3H), 3.88 (s, 3H), 3.76 (dd, 1H), 2.30-2.20 (m, 1H), 2.10-2.00 (m, 1H), 1.95 (s, 3H); (Yield: 20%)
1H NMR (400 MHz, CD3OD) δ 7.99 (d, 1H), 7.79 (s, 1H), 7.50 (d, 1H), 6.90-6.70 (m, 3H), 4.46 (t, 1H), 4.20-3.90 (m, 3H), 3.88 (s, 3H), 3.76 (dd, 1H), 2.35-2.20 (m, 1H), 2.15-2.00 (m, 1H), 1.95 (s, 3H); (Yield: 15%)
1H NMR (400 MHz, CD3OD) δ 8.02 (d, 1H), 7.38 (s, 1H), 7.02 (s, 2H), 6.85 (s, 1H), 6.81 (d, 1H), 4.46 (t, 1H), 4.30-4.00 (m, 3H), 3.88 (s, 3H), 3.84 (s, 3H), 3.84-3.75 (m, 1H), 2.35-2.20 (m, 1H), 2.14 (s, 3H), 2.10-2.00 (m, 1H), 1.95 (s, 3H); (Yield: 25%)
1H NMR (400 MHz, CD3OD) δ 8.25 (s, 1H), 8.04 (d, 1H), 7.66 (d, 1H), 7.27 (d, 1H), 6.87 (s, 1H), 6.82 (d, 1H), 4.49 (t, 1H), 4.30-4.00 (m, 3H), 3.89 (s, 3H), 3.85-3.80 (m, 1H), 2.42 (s, 3H), 2.35-2.25 (m, 1H), 2.15-2.00 (m, 1H), 1.95 (s, 3H); (Yield: 26%)
1H NMR (400 MHz, CD3OD) δ 8.79 (s, 1H), 8.07 (d, 1H), 8.02 (d, 1H), 7.23 (d, 1H), 6.88 (s, 1H), 6.81 (d, 1H), 4.47 (t, 1H), 4.25-3.90 (m, 3H), 3.89 (s, 3H), 3.85-3.80 (m, 1H), 2.48 (s, 3H), 2.35-2.20 (m, 1H), 2.15-2.00 (m, 1H), 1.95 (s, 3H); (Yield: 16%)
1H NMR (400 MHz, CD3OD) δ 8.01 (d, 1H), 7.88 (d, 1H), 7.75 (s, 1H), 7.45 (d, 1H), 6.94 (s, 1H), 6.83 (d, 1H), 4.48 (t, 1H), 4.20-3.95 (m, 3H), 3.89 (s, 3H), 3.85-3.75 (m, 1H), 2.35-2.25 (m, 1H), 2.15-2.00 (m, 1H), 1.96 (s, 3H); (Yield: 18%)
1H NMR (400 MHz, CDCl3) δ 8.52 (d, 1H), 7.90 (d, 1H), 7.78 (d, 1H), 6.99 (s, 1H), 6.82 (d, 1H), 6.01 (brs, 1H), 4.64 (brs, 1H), 4.20-3.92 (m, 2H), 3.92 (s, 3H), 3.90-3.70 (m, 2H), 2.62 (s, 3H), 2.35-2.20 (m, 1H), 2.20-2.05 (m, 1H), 2.03 (s, 3H); (Yield: 12%)
1H NMR (400 MHz, CD3OD) δ 8.09 (d, 1H), 7.41 (s, 1H), 7.22 (t, 1H), 7.13 (d, 1H), 6.91 (s, 1H), 6.64 (d, 1H), 4.49 (t, 1H), 4.30-4.00 (m, 3H), 3.91 (s, 3H), 3.91-3.84 (m, 1H), 3.84 (s, 3H), 2.35-2.25 (m, 1H), 2.15-2.00 (m, 1H), 1.95 (s, 3H); (Yield: 15%)
The titled compound was prepared as a pale yellow solid in the same manner as Example 137 by using (S)—N-{1-(2-chloropyrido[3,2-d]pyrimidin-4-yl)pyrrolidin-3-yl}acetamide prepared in Reference Example 45 and 3,5-diaminobenzonitrile.
1H NMR (400 MHz, CD3OD) δ 8.43 (d, 1H), 7.74 (d, 1H), 7.55-7.44 (m, 2H), 7.34 (s, 1H), 6.55 (s, 1H), 4.80-3.65 (m, 5H), 2.35-2.15 (m, 1H), 2.15-1.97 (m, 1H), 1.95 (s, 3H); (Yield: 29%)
A mixture of (S)-tert-butyl {1-(2-chloropyrido[3,2-d]pyrimidin-4-yl)pyrrolidin-3-yl}carbamate (20 mg, 0.06 mmol) prepared in Reference Example 46, 3,5-diaminobenzonitrile (8.8 mg, 0.07 mmol), palladium acetate (0.22 mg, 0.001 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthine (1.7 mg, 0.003 mmol), cesium carbonate (58.6 mg, 0.18 mmol) and anhydrous 1,4-dioxane (1 ml) was stirred for 1 hour in a microwave (600 W). After cooling the reaction solution to room temperature, the same was concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=20/1) and dissolved in ethyl acetate (1 ml), and then hydrochloric acid gas was added thereto. The resulting white solid was filtered to prepare the titled compound (3 mg).
1H NMR (400 MHz, CD3OD) δ 8.80 (s, 1H), 8.06 (s, 1H), 8.00-7.80 (m, 2H), 7.36 (s, 1H), 7.00-6.90 (m, 1H), 4.85-4.70 (m, 1H), 4.50-4.00 (m, 4H), 2.70-2.15 (m, 2H)
A mixture of (S)-tert-butyl {1-(2-chloropyrido[3,2-d]pyrimidin-4-yl)pyrrolidin-3-yl}(methyl)carbamate (20 mg, 0.06 mmol) prepared in Reference Example 47, 3,5-diaminobenzonitrile (8.8 mg, 0.07 mmol), palladium acetate (0.22 mg, 0.001 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthine (1.7 mg, 0.003 mmol), cesium carbonate (58.6 mg, 0.18 mmol) and anhydrous 1,4-dioxane (1 ml) was stirred for 1 hour in a microwave (600 W). After cooling the reaction solution to room temperature, the same was concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (dichloromethane/methanol=30/1) and dissolved in ethyl acetate (1 ml), and then hydrochloric acid gas was added thereto. The resulting white solid was filtered to prepare the titled compound (5 mg).
1H NMR (400 MHz, CD3OD) δ 8.81 (s, 1H), 8.09 (d, 2H), 7.87 (brs, 1H), 7.55 (s, 1H), 7.10-7.00 (m, 1H), 4.85-4.70 (m, 1H), 4.50-4.00 (m, 4H), 2.85 (s, 3H), 2.80-2.30 (m, 2H)
The titled compound was prepared as a pale yellow solid in the same manner as Example 157 by using (S)-tert-butyl {1-(2-chloropyrido[3,2-d]pyrimidin-4-yl)pyrrolidin-3-yl}(methyl)carbamate prepared in Reference Example 47 and 5-(trifluoromethyl)-1,3-phenylenediamine.
1H NMR (400 MHz, CD3OD) δ 8.80 (s, 1H), 8.15-7.85 (m, 4H), 7.38 (s, 1H), 5.10-4.90 (m, 1H), 4.50-3.80 (m, 4H), 2.95-2.75 (m, 3H), 2.70-2.15 (m, 2H); (Yield: 18%)
The titled compounds of Examples 159 to 163 were prepared in the same manner as Example 157 by reacting 3,5-diaminobenzonitrile, 5-(trifluoromethyl)-1,3-phenylenediamine, 2-(trifluoromethyl)-1,4-phenylenediamine, 4-chloro-1,3-diaminobenzene or 5-amino-2-methylbenzonitrile respectively with (S)-tert-butyl {1-(2-chloropyrido[3,2-d]pyrimidin-4-yl)pyrrolidin-3-yl}(propyl)carbamate prepared in Reference Example 48.
1H NMR (400 MHz, CD3OD) δ 8.80 (s, 1H), 8.05 (d, 1H), 7.86 (s, 2H), 7.79 (s, 1H), 7.35 (s, 1H), 5.00-4.90 (m, 1H), 4.80-4.00 (m, 4H), 3.14 (t, 2H), 2.75-2.25 (m, 2H), 1.81 (t, 2H), 1.07 (t, 3H); (Yield: 23%)
1H NMR (400 MHz, CD3OD) δ 8.80 (s, 1H), 8.20-7.80 (m, 4H), 7.45 (s, 1H), 4.95 (brs, 1H), 4.80-4.00 (m, 4H), 3.12 (q, 2H), 2.60-2.30 (m, 2H), 1.82 (t, 2H), 1.06 (t, 3H); (Yield: 21%)
1H NMR (400 MHz, CD3OD) δ 8.74 (s, 1H), 8.05-7.95 (m, 1H), 7.90-7.75 (m, 2H), 7.55 (brs, 1H), 7.12 (d, 1H), 5.00-4.90 (m, 1H), 4.75-4.00 (m, 4H), 3.11 (q, 2H), 2.75-2.30 (m, 2H), 1.79 (q, 2H), 1.06 (t, 3H); (Yield: 15%)
1H NMR (400 MHz, CD3OD) δ 8.76 (s, 1H), 8.01 (brs, 1H), 7.82 (brs, 1H), 7.50-7.30 (m, 2H), 7.25-7.10 (m, 1H), 5.00-4.90 (m, 1H), 4.75-4.00 (m, 4H), 3.12 (q, 2H), 2.70-2.25 (m, 2H), 1.78 (q, 2H), 1.07 (t, 3H); (Yield: 18%)
1H NMR (400 MHz, CD3OD) δ 8.77 (s, 1H), 8.10-8.00 (m, 2H), 7.83 (s, 1H), 7.73 (dd, 1H), 7.51 (s, 1H), 5.00-4.90 (m, 1H), 4.75-4.00 (m, 4H), 3.20-3.00 (m, 2H), 2.70-2.55 (m, 1H), 2.55 (s, 3H), 2.54-2.25 (m, 1H), 1.90-1.70 (m, 2H), 1.10-1.00 (m, 3H); (Yield: 28%)
The titled compound was prepared as a pale yellow solid in the same manner as Example 157 by using (S)-tert-butyl {1-(2-chloropyrido[3,2-d]pyrimidin-4-yl)pyrrolidin-3-yl}(pentyl)carbamate prepared in Reference Example 49 and 3,5-diaminobenzonitrile.
1H NMR (400 MHz, CD3OD) δ 8.80 (s, 1H), 8.07 (s, 1H), 7.99 (s, 2H), 7.85 (s, 1H), 7.45 (s, 1H), 4.80-4.00 (m, 5H), 3.17 (brs, 2H), 2.75-2.25 (m, 2H), 1.79 (brs, 2H), 1.43 (brs, 4H), 0.96 (brs, 3H); (Yield: 25%)
The titled compound was prepared as a pale yellow solid in the same manner as Example 157 by using (R)—N-{1-(2-chloropyrido[3,2-d]pyrimidin-4-yl)piperidin-3-yl}acetamide prepared in Reference Example 50 and 3,5-diaminobenzonitrile.
1H NMR (400 MHz, CD3OD) δ 8.45 (s, 1H), 7.83 (d, 1H), 7.60-7.50 (m, 1H), 7.44 (s, 1H), 7.33 (s, 1H), 6.58 (s, 1H), 5.00-4.90 (m, 1H), 4.20-3.70 (m, 4H), 2.10-1.90 (m, 2H), 1.89 (s, 3H), 1.85-1.65 (m, 2H); (Yield: 30%)
The titled compounds of Examples 166 and 167 were prepared in the same manner as Example 156 by reacting 3,5-diaminobenzonitrile and 5-(trifluoromethyl)-1,3-phenylenediamine respectively with (R)-tert-butyl {1-(2-chloropyrido[3,2-d]pyrimidin-4-yl)piperidin-3-yl}carbamate prepared in Reference Example 51.
1H NMR (400 MHz, CD3OD) δ 8.79 (s, 1H), 8.08 (d, 1H), 7.90-7.80 (m, 1H), 7.70-7.50 (m, 1H), 7.40-7.30 (m, 1H), 7.00-6.85 (m, 1H), 5.70-5.50 (m, 1H), 5.00-4.80 (m, 1H), 4.40-3.90 (m, 2H), 3.67 (brs, 1H), 2.27 (brs, 1H), 2.07 (brs, 1H), 2.00-1.85 (m, 2H); (Yield: 28%)
1H NMR (400 MHz, CD3OD) δ 8.80 (s, 1H), 8.10 (d, 1H), 8.00-7.80 (m, 2H), 7.68 (s, 1H), 7.40 (s, 1H), 5.54 (brs, 1H), 4.90-4.70 (m, 1H), 4.42 (brs, 1H), 4.15 (brs, 1H), 3.69 (brs, 1H), 2.27 (brs, 1H), 2.10-1.80 (m, 3H); (Yield: 18%)
n-Butanol (1 ml) solution of (S)—N-{1-(2-chloro-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl)pyrrolidin-3-yl}acetamide (30 mg, 0.11 mmol) prepared in Reference Example 52 and 3,5-diaminobenzonitrile (15.7 mg, 0.12 mmol) was stirred at 130° C. overnight. After cooling the reaction solution to room temperature, ethyl acetate (1 ml) solution was added thereto and they were stirred for 2 hours. The resulting white solid was filtered to prepare the titled compound (37 mg).
1H NMR (400 MHz, CD3OD) δ 7.16 (s, 1H), 7.07 (s, 1H), 6.74 (s, 1H), 4.42 (brs, 1H), 4.30-3.60 (m, 4H), 3.16 (brs, 2H), 2.91 (brs, 2H), 2.26 (brs, 1H), 2.16 (brs, 2H), 2.03 (brs, 1H), 1.95 (s, 3H)
The titled compounds of Examples 169 to 173 were prepared in the same manner as Example 168 by reacting 5-(trifluoromethyl)-1,3-phenylenediamine, 4-chloro-1,3-diaminobenzene, 5-amino-2-methylbenzonitrile, 4-methyl-3-(trifluoromethyl)aniline or 2-nitro-1,4-phenylenediamine respectively with (S)—N-{1-(2-chloro-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl)pyrrolidin-3-yl}acetamide prepared in Reference Example 52.
1H NMR (400 MHz, CD3OD) δ 7.25 (s, 1H), 6.99 (s, 1H), 6.73 (s, 1H), 4.43 (brs, 1H), 4.30-3.70 (m, 4H), 3.15 (brs, 2H), 2.90 (brs, 2H), 2.25 (brs, 1H), 2.16 (brs, 2H), 2.02 (brs, 1H), 1.95 (s, 3H); (Yield: 48%)
1H NMR (400 MHz, CD3OD) δ 7.19 (s, 1H), 7.02 (s, 1H), 6.75 (s, 1H), 4.40 (brs, 1H), 4.30-3.65 (m, 4H), 3.19 (brs, 2H), 2.88 (brs, 2H), 2.40-2.00 (m, 4H), 1.96 (s, 3H); (Yield: 36%)
1H NMR (400 MHz, CD3OD) δ 7.97 (s, 1H), 7.68 (s, 1H), 7.44 (s, 1H), 4.43 (brs, 1H), 4.30-3.60 (m, 4H), 3.17 (brs, 2H), 2.92 (brs, 2H), 2.52 (s, 3H), 2.35-2.00 (m, 4H), 1.95 (s, 3H); (Yield: 59%)
1H NMR (400 MHz, CD3OD) δ 8.15-8.00 (m, 1H), 7.61 (d, 1H), 7.39 (d, 1H), 4.44 (brs, 1H), 4.25-3.60 (m, 4H), 3.17 (brs, 2H), 2.93 (t, 2H), 2.46 (s, 3H), 2.35-2.00 (m, 4H), 1.96 (s, 3H); (Yield: 52%)
1H NMR (400 MHz, CD3OD) δ 8.51 (s, 1H), 7.40 (d, 1H), 7.01 (d, 1H), 4.42 (brs, 1H), 4.25-3.60 (m, 4H), 3.20-3.10 (m, 2H), 2.95-2.85 (m, 2H), 2.35-2.00 (m, 4H), 1.95 (s, 3H); (Yield: 30%)
A mixture of (S)-tert-butyl {1-(2-chloro-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl)pyrrolidin-3-yl}carbamate (34 mg, 0.1 mmol) prepared in Reference Example 53, 3,5-diaminobenzonitrile (16 mg, 0.12 mmol), palladium acetate (0.22 mg, 0.001 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthine (1.74 mg, 0.003 mmol), cesium carbonate (97.8 mg, 0.3 mmol) and anhydrous 1,4-dioxane (1 ml) was stirred at 120° C. overnight. After cooling the reaction solution to room temperature, the same was concentrated under reduced pressure. The resulting residue was purified with silica gel column chromatography (n-hexane/ethyl acetate=2/1) and dissolved in ethyl acetate (1 ml) and methanol (1 ml), and then hydrochloric acid gas was added thereto. The resulting white solid was filtered to prepare the titled compound (20 mg).
1H NMR (400 MHz, CD3OD) δ 7.90-7.60 (m, 2H), 7.23 (s, 1H), 4.40-3.80 (m, 5H), 3.25-3.15 (m, 2H), 2.98 (t, 2H), 2.60-2.40 (m, 1H), 2.30-2.10 (m, 3H)
The titled compounds of Examples 175 to 178 were prepared in the same manner as Example 174 by reacting 5-(trifluoromethyl)-1,3-phenylenediamine, 4-chloro-1,3-diaminobenzene, 5-amino-2-methylbenzonitrile or 4-methyl-3-(trifluoromethyl)aniline respectively with (S)-tert-butyl {1-(2-chloro-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl)pyrrolidin-3-yl}carbamate prepared in Reference Example 53.
1H NMR (400 MHz, CD3OD) δ 8.15-7.85 (m, 2H), 7.40 (s, 1H), 4.40-3.85 (m, 5H), 3.24 (brs, 2H), 3.00 (brs, 2H), 2.60-2.40 (m, 1H), 2.35-2.10 (m, 3H); (Yield: 35%)
1H NMR (400 MHz, CD3OD) δ 7.40-7.20 (m, 2H), 7.10-6.95 (m, 1H), 4.30-3.80 (m, 5H), 3.19 (brs, 2H), 3.00-2.90 (m, 2H), 2.55-2.30 (m, 1H), 2.30-2.10 (m, 3H); (Yield: 25%)
1H NMR (400 MHz, CD3OD) δ 7.95 (s, 1H), 7.69 (d, 1H), 7.45 (d, 1H), 4.30-3.90 (m, 5H), 3.21 (t, 2H), 2.96 (t, 2H), 2.52 (s, 3H), 2.52-2.50 (m, 1H), 2.30-2.10 (m, 3H); (Yield: 45%)
1H NMR (400 MHz, CD3OD) δ 8.01 (d, 1H), 7.70-7.60 (m, 1H), 7.41 (t, 1H), 4.40-3.80 (m, 5H), 3.25-3.10 (m, 2H), 3.00-2.90 (m, 2H), 2.55-2.40 (m, 1H), 2.47 (s, 3H), 2.35-2.10 (m, 3H); (Yield: 42%)
The titled compounds of Examples 179 to 183 were prepared in the same manner as Example 174 by reacting 3,5-diaminobenzonitrile, 5-(trifluoromethyl)-1,3-phenylenediamine, 4-chloro-1,3-diaminobenzene, 5-amino-2-methylbenzonitrile or 4-methyl-3-(trifluoromethyl)aniline respectively with (S)-tert-butyl {1-(2-chloro-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl)pyrrolidin-3-yl}(methyl)carbamate prepared in Reference Example 54.
1H NMR (400 MHz, CD3OD) δ 8.15-8.00 (m, 2H), 7.47 (s, 1H), 4.40-3.80 (m, 5H), 3.24 (t, 2H), 3.00 (t, 2H), 2.82 (s, 3H), 2.65-2.30 (m, 2H), 2.30-2.15 (m, 2H); (Yield: 45%)
1H NMR (400 MHz, CD3OD) δ 8.20-7.90 (m, 2H), 7.43 (s, 1H), 4.40-3.80 (m, 5H), 3.22 (brs, 2H), 3.00 (brs, 2H), 2.80 (s, 3H), 2.65-2.30 (m, 2H), 2.02 (brs, 2H); (Yield: 41%)
1H NMR (400 MHz, CD3OD) δ 8.15-7.85 (m, 1H), 7.85-7.50 (m, 2H), 4.40-3.80 (m, 5H), 3.22 (brs, 2H), 2.96 (brs, 2H), 2.78 (d, 3H), 2.54 (brs, 1H), 2.39 (brs, 1H), 2.20 (brs, 2H); (Yield: 25%)
1H NMR (400 MHz, CD3OD) δ 8.02 (s, 1H), 7.65 (s, 1H), 7.46 (d, 1H), 4.40-3.80 (m, 5H), 3.22 (brs, 2H), 2.97 (t, 2H), 2.81 (s, 3H), 2.52 (s, 3H), 2.52-2.40 (m, 1H), 2.40-2.30 (m, 1H), 2.21 (brs, 2H); (Yield: 52%)
1H NMR (400 MHz, CD3OD) δ 8.03 (d, 1H), 7.70-7.60 (m, 1H), 7.42 (t, 1H), 4.40-3.70 (m, 5H), 3.21 (brs, 2H), 2.96 (brs, 2H), 2.79 (d, 3H), 2.54 (brs, 1H), 2.47 (s, 3H), 2.35 (brs, 1H), 2.25-2.10 (m, 2H); (Yield: 45%)
The titled compounds of Examples 184 to 189 were prepared in the same manner as Example 174 by reacting 3,5-diaminobenzonitrile, 5-(trifluoromethyl)-1,3-phenylenediamine, 2-(trifluoromethyl)-1,4-phenylenediamine, 4-chloro-1,3-diaminobenzene, 4-methyl-3-(trifluoromethyl)aniline or 5-chloro-1,3-diaminobenzene respectively with (S)-tert-butyl {1-(2-chloro-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl)pyrrolidin-3-yl}(propyl)carbamate prepared in Reference Example 55.
1H NMR (400 MHz, CD3OD) δ 8.01 (s, 1H), 7.90 (s, 1H), 7.38 (s, 1H), 4.45-3.90 (m, 5H), 3.23 (brs, 2H), 3.11 (t, 2H), 2.99 (brs, 2H), 2.65-2.30 (m, 2H), 2.21 (brs, 2H), 1.80 (brs, 2H), 1.06 (t, 3H); (Yield: 42%)
1H NMR (400 MHz, CD3OD) δ 8.25-7.95 (m, 2H), 7.46 (s, 1H), 4.50-3.80 (m, 5H), 3.25 (brs, 2H), 3.10 (brs, 2H), 3.00 (brs, 2H), 2.65-2.30 (m, 2H), 2.21 (brs, 2H), 1.81 (brs, 2H), 1.06 (t, 3H); (Yield: 35%)
1H NMR (400 MHz, CD3OD) δ 8.15-7.25 (m, 3H), 4.50-3.70 (m, 5H), 3.21 (brs, 2H), 3.08 (brs, 2H), 2.94 (brs, 2H), 2.54 (brs, 1H), 2.38 (brs, 1H), 2.18 (brs, 2H), 1.80 (brs, 2H), 1.10-0.90 (m, 3H); (Yield: 29%)
1H NMR (400 MHz, CD3OD) δ 7.80 (s, 1H), 7.60-7.35 (m, 2H), 4.50-3.70 (m, 5H), 3.19 (brs, 2H), 3.09 (brs, 2H), 2.95 (brs, 2H), 2.54 (brs, 1H), 2.41 (brs, 1H), 2.18 (brs, 2H), 1.80 (brs, 2H), 1.06 (t, 3H); (Yield: 28%)
1H NMR (400 MHz, CD3OD) δ 8.10-8.00 (m, 1H), 7.59 (d, 1H), 7.42 (t, 1H), 4.50-3.70 (m, 5H), 3.21 (brs, 2H), 3.08 (brs, 2H), 2.96 (brs, 2H), 2.55-2.48 (m, 1H), 2.47 (s, 3H), 2.31 (brs, 1H), 2.20 (brs, 2H), 1.77 (brs, 2H), 1.05 (t, 3H); (Yield: 45%)
1H NMR (400 MHz, CD3OD) δ 7.85-7.60 (m, 2H), 7.16 (s, 1H), 4.50-3.70 (m, 5H), 3.22 (brs, 2H), 3.10 (brs, 2H), 2.97 (d, 2H), 2.54 (brs, 1H), 2.37 (brs, 1H), 2.20 (brs, 2H), 1.82 (t, 2H), 1.06 (t, 3H); (Yield: 32%)
The titled compounds of Examples 190 to 198 were prepared in the same manner as Example 168 by reacting 3,5-diaminobenzonitrile, 5-(trifluoromethyl)-1,3-phenylenediamine, 2-(trifluoromethyl)-1,4-phenylenediamine, 4-chloro-1,3-diaminobenzene, 5-amino-2-methylbenzonitrile, 4-methyl-3-(trifluoromethyl)aniline, 4-fluoro-3-trifluoromethylphenylamine, 5-chloro-1,3-diaminobenzene or 2-nitro-1,4-phenylenediamine respectively with (R)—N-{1-(2-chloro-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl)piperidin-3-yl}acetamide prepared in Reference Example 56.
1H NMR (400 MHz, CD3OD) δ 7.06 (s, 1H), 6.99 (s, 1H), 6.71 (s, 1H), 4.59 (d, 1H), 4.37 (d, 1H), 3.85 (brs, 1H), 3.37 (d, 1H), 3.19 (t, 2H), 3.10-3.00 (m, 1H), 2.91 (t, 2H), 2.19 (t, 2H), 2.03 (brs, 1H), 1.97 (s, 3H), 1.93 (brs, 1H), 1.68 (q, 2H); (Yield: 35%)
1H NMR (400 MHz, CD3OD) δ 7.09 (s, 1H), 6.93 (s, 1H), 6.73 (s, 1H), 4.54 (d, 1H), 4.38 (d, 1H), 3.85 (s, 1H), 3.37 (t, 1H), 3.30-3.15 (m, 2H), 3.10-3.00 (m, 1H), 2.91 (t, 2H), 2.18 (t, 2H), 2.03 (brs, 1H), 1.95 (s, 3H), 1.90 (brs, 1H), 1.67 (brs, 2H); (Yield: 40%)
1H NMR (400 MHz, CD3OD) δ 7.51 (s, 1H), 7.29 (d, 1H), 6.87 (d, 1H), 4.46 (d, 1H), 4.35 (d, 1H), 3.82 (s, 1H), 3.35-3.30 (m, 1H), 3.20 (t, 2H), 3.05-2.95 (m, 1H), 2.87 (t, 2H), 2.16 (t, 2H), 2.01 (brs, 1H), 1.94 (s, 3H), 1.87 (brs, 1H), 1.63 (t, 2H); (Yield: 25%)
1H NMR (400 MHz, CD3OD) δ 7.17 (d, 1H), 6.96 (s, 1H), 6.65 (d, 1H), 4.54 (brs, 1H), 4.35 (brs, 1H), 3.85 (brs, 1H), 3.36 (brs, 1H), 3.20 (t, 2H), 3.03 (brs, 1H), 2.88 (brs, 2H), 2.16 (t, 2H), 2.03 (brs, 1H), 1.96 (s, 3H), 1.91 (brs, 1H), 1.66 (brs, 2H); (Yield: 23%)
1H NMR (400 MHz, CD3OD) δ 7.89 (s, 1H), 7.60 (d, 1H), 7.43 (d, 1H), 4.51 (d, 1H), 4.35 (d, 1H), 3.84 (s, 1H), 3.36 (t, 1H), 3.25-3.15 (m, 2H), 3.10-3.00 (m, 1H), 2.93 (t, 2H), 2.52 (s, 3H), 2.18 (t, 2H), 2.03 (brs, 1H), 1.95 (s, 3H), 1.92 (brs, 1H), 1.66 (brs, 2H); (Yield: 46%)
1H NMR (400 MHz, CD3OD) δ 7.91 (s, 1H), 7.54 (d, 1H), 7.39 (d, 1H), 4.49 (d, 1H), 4.38 (d, 1H), 3.84 (brs, 1H), 3.35 (t, 1H), 3.30-3.15 (m, 2H), 3.10-3.00 (m, 1H), 2.93 (t, 2H), 2.47 (s, 3H), 2.18 (t, 2H), 2.02 (brs, 1H), 1.94 (s, 3H), 1.89 (brs, 1H), 1.65 (t, 2H); (Yield: 44%)
1H NMR (400 MHz, CD3OD) δ 7.95 (s, 1H), 7.71 (brs, 1H), 7.37 (t, 1H), 4.49 (d, 1H), 4.35 (d, 1H), 3.83 (brs, 1H), 3.40-3.30 (m, 1H), 3.30-3.15 (m, 2H), 3.10-3.00 (m, 1H), 2.94 (t, 2H), 2.19 (t, 2H), 2.02 (brs, 1H), 1.94 (s, 3H), 1.89 (brs, 1H), 1.65 (t, 2H); (Yield: 51%)
1H NMR (400 MHz, CD3OD) δ 6.76 (s, 1H), 6.67 (s, 1H), 6.50 (s, 1H), 4.56 (d, 1H), 4.39 (d, 1H), 3.86 (brs, 1H), 3.40-3.30 (m, 1H), 3.25-3.10 (m, 2H), 3.10-3.00 (m, 1H), 2.89 (t, 2H), 2.25-2.15 (m, 2H), 2.05 (brs, 1H), 1.95 (s, 3H), 1.95-1.90 (m, 1H), 1.80-1.60 (m, 2H); (Yield: 24%)
1H NMR (400 MHz, CD3OD) δ 8.33 (s, 1H), 7.38 (d, 1H), 7.00 (d, 1H), 4.55 (brs, 1H), 4.42 (d, 1H), 3.82 (brs, 1H), 3.40-3.30 (m, 1H), 3.30-3.10 (m, 2H), 3.10-3.00 (m, 1H), 2.89 (t, 2H), 2.17 (t, 2H), 2.03 (brs, 1H), 1.94 (s, 3H), 1.90 (brs, 1H), 1.67 (q, 2H); (Yield: 22%)
The titled compounds of Examples 199 to 203 were prepared in the same manner as Example 174 by reacting 3,5-diaminobenzonitrile, 5-(trifluoromethyl)-1,3-phenylenediamine, 2-(trifluoromethyl)-1,4-phenylenediamine, 4-chloro-1,3-diaminobenzene or 2-nitro-1,4-phenylenediamine respectively with (R)-tert-butyl {1-(2-chloro-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl)piperidin-3-yl}carbamate prepared in Reference Example 57.
1H NMR (400 MHz, CD3OD) δ 7.91 (s, 1H), 7.80 (s, 1H), 7.41 (s, 1H), 4.59 (d, 1H), 4.32 (brs, 1H), 3.75-3.40 (m, 3H), 3.16 (brs, 2H), 3.00 (brs, 2H), 2.30-2.15 (m, 3H), 2.05-1.95 (m, 1H), 1.90-1.75 (m, 2H); (Yield: 25%)
1H NMR (400 MHz, CD3OD) δ 8.07 (s, 1H), 7.89 (s, 1H), 7.42 (s, 1H), 4.56 (d, 1H), 4.35 (brs, 1H), 3.75-3.35 (m, 3H), 3.30-3.10 (m, 2H), 3.10-2.95 (m, 2H), 2.30-2.15 (m, 3H), 2.00-1.90 (m, 1H), 1.90-1.70 (m, 2H); (Yield: 26%)
1H NMR (400 MHz, CD3OD) δ 8.00 (s, 1H), 7.76 (d, 1H), 7.51 (d, 1H), 4.54 (d, 1H), 4.35 (brs, 1H), 3.75-3.30 (m, 3H), 3.30-3.05 (m, 2H), 3.05-2.90 (m, 2H), 2.30-2.10 (m, 3H), 2.00-1.60 (m, 3H); (Yield: 18%)
1H NMR (400 MHz, CD3OD) δ 7.72 (s, 1H), 7.62 (s, 1H), 7.52 (d, 1H), 4.54 (d, 1H), 4.24 (brs, 1H), 3.80-3.40 (m, 3H), 3.30-3.05 (m, 2H), 3.05-2.95 (m, 2H), 2.30-2.15 (m, 3H), 2.00-1.65 (m, 3H); (Yield: 20%)
1H NMR (400 MHz, CD3OD) δ 8.70 (s, 1H), 7.37 (d, 1H), 7.05 (d, 1H), 4.68 (brs, 1H), 4.35 (brs, 1H), 3.70-3.30 (m, 3H), 3.25-3.05 (m, 2H), 3.05-2.95 (m, 2H), 2.30-2.10 (m, 3H), 1.96 (brs, 1H), 1.76 (brs, 2H); (Yield: 15%)
The titled compounds of Examples 204 to 211 were prepared in the same manner as Example 174 by reacting 3,5-diaminobenzonitrile, 5-(trifluoromethyl)-1,3-phenylenediamine, 2-(trifluoromethyl)-1,4-phenylenediamine, 4-chloro-1,3-diaminobenzene, 5-chloro-1,3-diaminobenzene, 5-amino-2-methylbenzonitrile, 4-methyl-3-(trifluoromethyl)aniline or 2-nitro-1,4-phenylenediamine respectively with (R)-tert-butyl {1-(2-chloro-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl)piperidin-3-yl}(methyl)carbamate prepared in Reference Example 58.
1H NMR (400 MHz, CD3OD) δ 7.89 (s, 1H), 7.80 (s, 1H), 7.44 (s, 1H), 4.50 (d, 1H), 4.16 (brs, 1H), 3.86 (brs, 1H), 3.66 (brs, 1H), 3.43 (brs, 1H), 3.25-3.10 (m, 2H), 3.00 (brs, 2H), 2.69 (s, 3H), 2.30-2.15 (m, 3H), 2.05-1.65 (m, 3H); (Yield: 42%)
1H NMR (400 MHz, CD3OD) δ 7.96 (s, 1H), 7.77 (s, 1H), 7.41 (s, 1H), 4.45 (brs, 1H), 4.30-3.60 (m, 3H), 3.41 (brs, 1H), 3.25-3.10 (m, 2H), 3.05-2.95 (m, 2H), 2.64 (s, 3H), 2.23 (brs, 3H), 2.05-1.65 (m, 3H); (Yield: 40%)
1H NMR (400 MHz, CD3OD) δ 7.83 (s, 1H), 7.66 (d, 1H), 7.36 (d, 1H), 4.39 (brs, 1H), 4.20-3.50 (m, 3H), 3.35 (brs, 1H), 3.14 (brs, 2H), 2.00-2.90 (m, 2H), 2.62 (s, 3H), 2.30-2.10 (m, 3H), 2.05-1.65 (m, 3H); (Yield: 25%)
1H NMR (400 MHz, CD3OD) δ 7.58 (s, 2H), 7.41 (d, 1H), 4.41 (brs, 1H), 4.25-3.55 (m, 3H), 3.38 (brs, 1H), 3.14 (d, 2H), 2.97 (t, 2H), 2.64 (s, 3H), 2.30-2.10 (m, 3H), 2.05-1.65 (m, 3H); (Yield: 22%)
1H NMR (400 MHz, CD3OD) δ 7.65 (s, 1H), 7.48 (s, 1H), 7.19 (s, 1H), 4.48 (d, 1H), 4.16 (brs, 1H), 3.89 (brs, 1H), 3.63 (brs, 1H), 3.41 (brs, 1H), 3.16 (d, 2H), 2.98 (t, 2H), 2.67 (s, 3H), 2.30-2.15 (m, 3H), 2.05-1.65 (m, 3H); (Yield: 29%)
1H NMR (400 MHz, CD3OD) δ 7.85 (s, 1H), 7.63 (d, 1H), 7.49 (d, 1H), 4.44 (brs, 1H), 4.12 (brs, 1H), 3.81 (brs, 1H), 3.64 (brs, 1H), 3.35 (brs, 1H), 3.14 (d, 2H), 2.96 (t, 2H), 2.64 (s, 3H), 2.53 (s, 3H), 2.30-2.15 (m, 3H), 2.05-1.65 (m, 3H); (Yield: 40%)
1H NMR (400 MHz, CD3OD) δ 7.82 (s, 1H), 7.58 (s, 1H), 7.45 (d, 1H), 4.40 (brs, 1H), 4.12 (brs, 1H), 3.87 (brs, 1H), 3.63 (brs, 1H), 3.31 (brs, 1H), 3.14 (brs, 2H), 2.95 (d, 2H), 2.59 (s, 3H), 2.48 (s, 3H), 2.30-2.15 (m, 3H), 2.00-1.65 (m, 3H); (Yield: 38%)
1H NMR (400 MHz, CD3OD) δ 8.30 (s, 1H), 7.40 (d, 1H), 7.06 (d, 1H), 4.44 (brs, 1H), 4.30-3.50 (m, 3H), 3.35-3.30 (m, 1H), 3.20-3.05 (m, 2H), 3.05-2.90 (m, 2H), 2.65 (s, 3H), 2.30-2.10 (m, 3H), 2.05-1.65 (m, 3H); (Yield: 26%)
The titled compounds of Examples 212 to 218 were prepared in the same manner as Example 174 by reacting 3,5-diaminobenzonitrile, 5-(trifluoromethyl)-1,3-phenylenediamine, 4-chloro-1,3-diaminobenzene, 5-chloro-1,3-diaminobenzene, 5-amino-2-methylbenzonitrile, 4-methyl-3-(trifluoromethyl)aniline or 2-nitro-1,4-phenylenediamine respectively with (R)-tert-butyl {1-(2-chloro-5,6,7,8-tetrahydroquinazolin-4-yl)piperidin-3-yl}(methyl)carbamate prepared in Reference Example 59.
1H NMR (400 MHz, CD3OD) δ 7.72 (s, 1H), 7.59 (s, 1H), 7.26 (s, 1H), 4.45 (d, 1H), 4.08 (d, 1H), 3.70-3.35 (m, 3H), 2.82 (t, 2H), 2.80-2.60 (m, 5H), 2.30-2.20 (m, 1H), 2.05-1.70 (m, 5H); (Yield: 35%)
1H NMR (400 MHz, CD3OD) δ 7.91 (s, 1H), 7.80 (s, 1H), 7.39 (s, 1H), 4.40 (d, 1H), 4.08 (d, 1H), 3.68 (t, 1H), 3.50-3.30 (m, 2H), 2.83 (t, 2H), 2.80-2.60 (m, 5H), 2.25 (brs, 1H), 2.05-1.70 (m, 7H); (Yield: 30%)
1H NMR (400 MHz, CD3OD) δ 7.65-7.55 (m, 2H), 7.38 (t, 1H), 4.37 (d, 1H), 4.02 (d, 1H), 3.65 (t, 1H), 3.48 (t, 1H), 3.36 (brs, 1H), 2.80 (t, 2H), 2.75-2.55 (m, 5H), 2.23 (brs, 1H), 2.00-1.70 (m, 7H); (Yield: 20%)
1H NMR (400 MHz, CD3OD) δ 7.34 (s, 1H), 7.17 (s, 1H), 6.94 (s, 1H), 4.42 (d, 1H), 4.08 (d, 1H), 3.60 (t, 1H), 3.46 (t, 1H), 3.35 (brs, 1H), 2.80 (t, 2H), 2.75-2.55 (m, 5H), 2.30-2.20 (m, 1H), 2.00-1.70 (m, 7H); (Yield: 26%)
1H NMR (400 MHz, CD3OD) δ 7.91 (s, 1H), 7.64 (d, 1H), 7.48 (d, 1H), 4.40 (d, 1H), 4.08 (d, 1H), 3.55 (t, 1H), 3.43 (t, 1H), 3.34 (brs, 1H), 2.80 (t, 2H), 2.78-2.55 (m, 5H), 2.53 (s, 3H), 2.24 (brs, 1H), 2.00-1.70 (m, 7H); (Yield: 46%)
1H NMR (400 MHz, CD3OD) δ 7.85 (s, 1H), 7.60 (d, 1H), 7.45 (d, 1H), 4.35 (d, 1H), 4.08 (d, 1H), 3.59 (t, 1H), 3.45 (t, 1H), 3.31 (brs, 1H), 2.80 (t, 2H), 2.78-2.55 (m, 5H), 2.48 (s, 3H), 2.22 (brs, 1H), 2.00-1.70 (m, 7H); (Yield: 41%)
1H NMR (400 MHz, CD3OD) δ 8.37 (s, 1H), 7.39 (d, 1H), 7.05 (d, 1H), 4.40 (d, 1H), 4.08 (d, 1H), 3.52 (t, 1H), 3.45 (t, 1H), 3.33 (brs, 1H), 2.77 (t, 2H), 2.75-2.55 (m, 5H), 2.23 (brs, 1H), 2.00-1.65 (m, 7H); (Yield: 22%)
The titled compounds of Examples 219 to 224 were prepared in the same manner as Example 168 by reacting 3,5-diaminobenzonitrile, 5-(trifluoromethyl)-1,3-phenylenediamine, 2-(trifluoromethyl)-1,4-phenylenediamine, 5-chloro-1,3-diaminobenzene, 4-methyl-3-(trifluoromethyl)aniline or 2-nitro-1,4-phenylenediamine respectively with (R)-1-(2-chloro-6,7-dihydro-5H-cyclopenta[d]pyrimidin-4-yl)-N-methylpiperidine-3-carboxamide prepared in Reference Example 60.
1H NMR (400 MHz, CD3OD) δ 7.03 (d, 2H), 6.74 (s, 1H), 4.59 (brs, 1H), 4.38 (d, 1H), 3.39 (t, 2H), 3.08 (brs, 2H), 2.91 (brs, 2H), 2.72 (s, 3H), 2.52 (brs, 1H), 2.25-2.15 (m, 2H), 2.10-2.00 (m, 1H), 1.95-1.80 (m, 2H), 1.70-1.60 (m, 1H); (Yield: 47%)
1H NMR (400 MHz, CD3OD) δ 7.10 (s, 1H), 6.93 (s, 1H), 6.74 (s, 1H), 4.60 (brs, 1H), 4.40 (d, 1H), 3.50-3.30 (m, 2H), 3.07 (brs, 2H), 2.91 (t, 2H), 2.70 (s, 3H), 2.51 (brs, 1H), 2.17 (t, 2H), 2.01 (brs, 1H), 1.95-1.80 (m, 2H), 1.70-1.60 (m, 1H); (Yield: 45%)
1H NMR (400 MHz, CD3OD) δ 7.50 (s, 1H), 7.29 (d, 1H), 6.86 (d, 1H), 4.54 (brs, 1H), 4.39 (d, 1H), 3.45-3.30 (m, 2H), 3.05 (brs, 2H), 2.88 (brs, 2H), 2.71 (s, 3H), 2.47 (brs, 1H), 2.25-2.15 (m, 2H), 1.98 (brs, 1H), 1.95-1.80 (m, 2H), 1.70-1.50 (m, 1H); (Yield: 35%)
1H NMR (400 MHz, CD3OD) δ 6.80 (s, 1H), 6.67 (s, 1H), 6.48 (s, 1H), 4.65 (brs, 1H), 4.40 (d, 1H), 3.45-3.30 (m, 2H), 3.05 (brs, 2H), 2.89 (brs, 2H), 2.72 (s, 3H), 2.51 (brs, 1H), 2.16 (brs, 2H), 2.01 (brs, 1H), 2.00-1.80 (m, 2H), 1.64 (brs, 1H); (Yield: 38%)
1H NMR (400 MHz, CD3OD) δ 7.89 (s, 1H), 7.54 (d, 1H), 7.38 (d, 1H), 4.51 (brs, 1H), 4.37 (d, 1H), 3.55-3.30 (m, 2H), 3.09 (brs, 2H), 2.93 (t, 2H), 2.71 (s, 3H), 2.60-2.40 (m, 4H), 2.18 (t, 2H), 1.99 (brs, 1H), 1.95-1.80 (m, 2H), 1.70-1.60 (m, 1H); (Yield: 47%)
1H NMR (400 MHz, CD3OD) δ 8.29 (s, 1H), 7.38 (d, 1H), 7.00 (d, 1H), 4.55 (brs, 1H), 4.39 (d, 1H), 3.45-3.30 (m, 2H), 3.06 (brs, 2H), 2.89 (t, 2H), 2.71 (s, 3H), 2.47 (brs, 1H), 2.17 (t, 2H), 1.99 (d, 1H), 1.95-1.80 (m, 2H), 1.70-1.60 (m, 1H); (Yield: 25%)
As CHO-K1 cells stably expressing human 5-HT4(a), we used the GeneBlAzer HTR4-CRE-bla CHO-K1 cells (Invitrogen corp.). The cells were cultured in DMEM medium supplemented with 10% bovine fetal serum (FBS), 25 mM HEPES (pH 7.4), 600 μg/ml Hygromycin B, 0.1 mM non-essential amino acids, 100 unit/ml penicillin and 100 μg/ml streptomycin under the condition of 37° C. and 5% CO2. Subcultures were performed three times per a week, each being at less than 80% confluence. At the previous day before treating test compounds, the cells were collected by using 0.5% trypsin/EDTA, and then diluted with a DMEM supplemented with 1% FBS, 25 mM HEPES and 0.1 mM non-essential amino acids into 3.125×105 cells/ml. 32 μl of the diluted cells were added into 384-well plate (10,000 cells/well) and then incubated overnight. The compounds to be used as a test material and a control drug were prepared as 500× of the various final treating concentrations of the drug with 100% DMSO, and then treated to the medium after diluting them to 100-folds to be 1% of the final DMSO concentration equally. After culturing overnight, 8 μl of the medium having 1% of DMSO was added into the cell-free control well and the non-stimulating control well, respectively. 8 μl of the control drug or the test material (which had been prepared by diluting 100-folds with the medium as mentioned in the above) having 1% of DMSO, were added to the respective remaining wells. After culturing in an incubator for 5 hours, the wells of the 384-plate were treated with the substrate solution (8 μl/well) prepared according to the vendor's instruction (i.e. Invitrogen's instruction), and then incubated in the dark room for additional 2 hours. Agonistic activities on 5-HT4 receptor were evaluated, on the basis of fluorescent values of the cleavage-products, which is generated cAMP-concentration dependently per equal time by beta-lactamase. After exciting to 410 nm of wavelength by using Genios Pro Fluorescence Detector, we measured the fluorescence values at two emission wavelengths (first wavelength: 465 nm, second wavelength: 535 nm). Data were analyzed on the basis of the ratio of fluorescence intensities of each well at the respective wavelengths. For all plates, the concentrations-response curve (1 pM-100 nM) of the control drug (Tegaserod) was included. Each EC50 values of the test compounds were calculated by non-linear regression analysis using GraphPad Prism program, based on the concentration-reactivity values according to 8-different concentrations of the test compounds. The results were represented in Table 1 below.
As shown in Table 1, the compounds of the present invention have excellent activities as a 5-HT4 receptor agonist, and thus they can be usefully applied for preventing or treating of the dysfunction in gastrointestinal motility.
The compound according to the present invention, i.e., the bicyclic derivative comprising pyrimidine ring or pharmaceutically acceptable salt thereof act as a 5-HT4 receptor agonist, and thus can be usefully applied to the prevention or treatment of gastrointestinal diseases such as dysfunction in gastrointestinal motility, for example, gastroesophageal reflux disease (GERD), constipation, irritable bowel syndrome (IBS), dyspepsia, post-operative ileus, delayed gastric emptying, gastroparesis, intestinal pseudo-obstruction, drug-induced delayed transit, diabetic gastric atony and the like.
| Number | Date | Country | Kind |
|---|---|---|---|
| 10-2013-0058843 | May 2013 | KR | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/KR2014/004636 | 5/23/2014 | WO | 00 |
| Number | Date | Country | |
|---|---|---|---|
| 20160090374 A1 | Mar 2016 | US |
