Patent Application
20190233427
The present invention relates to a compound of formula I,
wherein
Now it has been found that the present compounds of formula I are modulators of γ-secretase, they may be useful for the treatment or prevention of a disease associated with the deposition of β-amyloid in the brain, in particular Alzheimer's disease, and other diseases such as cerebral amyloid angiopathy, hereditary cerebral hemorrhage with amyloidosis, Dutch-type (HCHWA-D), multi-infarct dementia, dementia pugilistica and Down syndrome.
Alzheimer's disease (AD) is the most common cause of dementia in later life. Pathologically, AD is characterized by the deposition of amyloid in extracellular plaques and intracellular neurofibrillary tangles in the brain. The amyloid plaques are mainly composed of amyloid peptides (Aβ peptides) which originate from the β-Amyloid Precursor Protein (APP) by a series of proteolytic cleavage steps. Several forms of APP have been identified of which the most abundant are proteins of 695, 751 and 770 amino acids length. They all arise from a single gene through differential splicing. The Aβ peptides are derived from the same domain of the APP.
Aβ peptides are produced from APP through the sequential action of two proteolytic enzymes termed β- and γ-secretase. β-Secretase cleaves first in the extracellular domain of APP just outside of the trans-membrane domain (TM) to produce a C-terminal fragment of APP (CTFβ) containing the TM- and cytoplasmatic domain. CTFβ is the substrate for γ-secretase which cleaves at several adjacent positions within the TM to produce the Aβ peptides and the cytoplasmic fragment. Various proteolytic cleavages mediated by γ-secretase result in Aβ peptides of different chain length, e.g. Aβ38, Aβ40 and Aβ42. The latter one is regarded to be the more pathogenic amyloid peptide because of its strong tendency to form neurotoxic aggregates.
The β-secretase is a typical aspartyl protease. The γ-secretase is a high molecular weight complex that consists of four essential subunits: Presenilin (PS, including PS1 and PS2), nicastrin, anterior pharynx defective 1 (APH-1), and presenilin enhancer 2 (PEN-2). The atomic structure of human γ-secretase at 3.4 Å resolution has been published (X. Bai, C. Yan, G. Yang, P. Lu, D. Ma, L. Sun, R. Zhou, S. H. W. Scheres, Y. Shi, Nature 2015, 525, pages 212-217. The presenilins are bearing the catalytic site and represent a group of atypical aspartyl proteases which cleave their substrates within the TM of and which are themselves polytopic membrane proteins. The other essential components of γ-secretase, nicastrin and the products of the aph1 and pen-2 genes are believed to be responsible for substrate recognition and recruitment. Proven substrates for γ-secretase are APP and the proteins of the Notch receptor family, however, γ-secretase has a loose substrate specificity and many further membrane proteins unrelated to APP and Notch have been reported to be cleaved by the γ-secretase in vitro.
The γ-secretase activity is absolutely required for the production of Aβ peptides. This has been shown both by genetic means, i.e., ablation of the presenilin genes and by low-molecular-weight inhibitory compounds. According to the amyloid cascade hypothesis for AD the production and deposition of Aβ is the ultimate cause for the disease. Therefore, it was believed that selective and potent inhibition of γ-secretase might be useful for the prevention and treatment of AD.
An alternative mode of treatment is the modulation of the γ-secretase activity which results in a selective reduction of the Aβ42 production. This will lead in an increase of shorter Aβ isoforms, such as Aβ38, Aβ37 or others, which have no or reduced capability for aggregation and plaque formation, and are not or less neurotoxic. Compounds which show this effect on modulating γ-secretase activity include certain non-steroidal anti-inflammatory drugs (NSAIDs) and related analogues (Weggen et al., Nature, 414 (2001) 212-16).
Thus, the compounds of this invention will be useful for the treatment or prevention of a disease associated with the deposition of β-amyloid in the brain, in particular Alzheimer's disease, and other diseases such as cerebral amyloid angiopathy, hereditary cerebral hemorrhage with amyloidosis, Dutch-type (HCHWA-D), multi-infarct dementia, dementia pugilistica and Down syndrome.
Numerous documents describe the current knowledge on γ-secretase modulation, for example the following publications:
As used herein, the term “lower alkyl” denotes a saturated straight- or branched-chain group containing from 1 to 7 carbon atoms, for example, methyl, ethyl, propyl, isopropyl, n-butyl, i-butyl, 2-butyl, t-butyl and the like. Preferred alkyl groups are groups with 1-4 carbon atoms.
As used herein, the term “lower alkyl substituted by halogen” denotes an alkyl group as defined above, wherein at least one hydrogen atom is replaced by halogen, for example CF3, CHF2, CH2F, CHFCF3, CH2CHF2, CH2CH2F, CH2C(CH3)2CF3, CH2CF2CF3, CH(CF3)2, CH2CF3, (CH2)2CF3, (CH2)3CF3, CH(CH3)CF3, CF2CF3, and the like. The preferred group is CF3.
The term “lower alkoxy” denotes a lower alkyl group as defined above, which group is connected via an O atom.
The term “lower alkoxy substituted by halogen” denotes a lower alkoxy group as defined above, wherein at least one hydrogen atom is replaced by halogen.
The term “halogen” denotes chlorine, iodine, fluorine and bromine.
The term “pharmaceutically acceptable acid addition salts” embraces salts with inorganic and organic acids, such as hydrochloric acid, nitric acid, sulfuric acid, phosphoric acid, citric acid, formic acid, fumaric acid, maleic acid, acetic acid, succinic acid, tartaric acid, methane-sulfonic acid, p-toluenesulfonic acid and the like.
Objects of the present invention are compounds of formula I, the use of such compounds for the preparation of medicaments for the treatment of Alzheimer's disease, cerebral amyloid angiopathy, hereditary cerebral hemorrhage with amyloidosis, Dutch-type (HCHWA-D), multi-infarct dementia, dementia pugilistica or Down syndrome, their manufacture and medicaments based on a compound of formula I in accordance with the invention.
Further objects of the present invention are all forms of optically pure enantiomers, racemates or diastereometric mixtures for compounds of formula I.
One object of the present invention is a compound of formula I-1,
wherein
One further object of the present invention is a compound of formula I-2,
wherein
One object of the present invention is a compound of formula I-3,
wherein
One object of the present invention is a compound of formula I-4,
wherein
One object of the present invention is a compound of formula I-5,
wherein
One object of the present invention is a compound of formula I-6,
wherein
Further objects of the present invention are compounds of formulas
wherein
The present compounds of formula I and their pharmaceutically acceptable salts can be prepared by methods known in the art, for example, by processes described below, which processes comprise
with a compound of formula 10-b
to a compound of formula I
wherein the substituents have the meaning as described above, and,
if desired, converting the compounds obtained into pharmaceutically acceptable acid addition salts;
In more detail, compounds of formula I and their intermediates may be prepared by schemes 1-3 and by the description of 48 specific examples.
The preparation of derivatives of general formula Ia with (m=1), where made according to the scheme 1. The synthesis of target compounds commenced by the nucleophilic addition of a commercially available substituted anilines 2 onto the diphenyl cyanocarbonimidate 3 leading to the intermediates 4 in a good yield. Upon N-alkylation with an electrophile [2-(2-bromoethoxy)tetrahydropyran] containing a protected alcohol moiety the compounds 5 were obtained. Upon reaction with hydrazine the corresponding triazoles 6 were obtained. Deprotection of the alcohol protected by tetrahydropyran (THP) group under standard acidic conditions afforded 7 in nearly quantitative yield, the alcohol precursor for the Mitsunobu reaction forming 8. A Buchwald coupling of 8 with a bromo derivatives of formula 10-a gave final compounds Ia. Alternatively, a Sandmeyer reaction with 8 led to the versatiles intermediates bromotriazole 9, which can easily undergo a Buchwald type reaction with different anilines of type 10-b affording also final products of formula Ia.
The preparation of derivatives of general formula Ib with (m=2), where made according to the scheme 2. The synthesis of target compounds commenced by the nucleophilic addition of a commercially available substituted anilines 2 onto the diphenyl cyanocarbonimidate 3 leading to the intermediates 4 in a good yield. Upon N-alkylation with an electrophile [2-(3-bromopropoxy)tetrahydropyran] containing a protected alcohol moiety the compounds 11 were obtained. Upon reaction with hydrazine the corresponding triazoles 12 were obtained. Deprotection of the alcohol protected by tetrahydropyran (THP) group under standard acidic conditions afforded 13 in nearly quantitative yield, the alcohol precursor for the Mitsunobu reaction forming 14. A Buchwald coupling of 14 with a bromo derivatives of formula 10-a gave final compounds Ib. Alternatively, a Sandmeyer reaction of 14 led to the versatiles intermediates bromotriazole 15, which can easily undergo a Buchwald type reaction with different anilines of type 10-b affording also final products of formula Ib.
The preparation of derivatives of general formula Ic with (m=3), where made according to the scheme 3. The synthesis of target compounds commenced by the nucleophilic addition of a commercially available substituted anilines 2 onto the diphenyl cyanocarbonimidate 3 leading to the intermediates 4 in a good yield. Upon N-alkylation with an electrophile [2-(4-bromobutoxy)tetrahydropyran] containing a protected alcohol moiety the compounds 16 were obtained. Upon reaction with hydrazine the corresponding triazoles 17 were obtained. Deprotection of the alcohol protected by tetrahydropyran (THP) group under standard acidic conditions afforded 18 in nearly quantitative yield, the alcohol precursor for the Mitsunobu reaction forming 19. A Buchwald coupling of 19 with a bromo derivatives of formula 10-a gave final compounds Ic. Alternatively, a Sandmeyer reaction of 19 led to the versatiles intermediates bromotriazole 20, which can easily undergo a Buchwald type reaction with different anilines of type 10-b affording final products of formula Ic.
The heterocycles halides are either commercial available, known in the literature so they can be prepared by methods known in the art or alternatively could be prepared as described in the specification.
The compounds were investigated in accordance with the test given hereinafter.
Human neuroglioma H4 cells overexpressing human APP695 with the Swedish double mutation (K595N/M596L) were plated at 30,000 cells/well/100 μl in 96-well plates in IMDM media containing 10% FCS, 0.2 mg/l Hygromycin B and incubated at 37° C., 5% CO2.
3-4 hr post plating, compounds are a diluted in media and 50 μl is added as 1.5-fold concentrate to achieve the final concentration. Compound incubation is performed for 24 hr. Final doses typically range from 4 μm down to 0.0013 μm in half-log steps resulting in a eight point dose response curve.
Appropriate controls using vehicle only and reference compound were applied to this assay. The final concentration of Me2SO was 0.4%.
After incubation at 37° C., 5% CO2, the supernatant was subjected to quantification of secreted Aβ42 by the means of an AlphaLisa assay kit (Human Amyloid beta 1-42 Kit: Cat # AL203C, Perkin Elmer). 20 μl of the cell culture supernatant was transferred to an assay plate. Then 10 μl of a mixture of the AlphaLisa coupled capture antibody and the biotinylated detection antibody was added and incubated for 3 hours at room temperature while softly shaking the assay plate. After a further addition of 20 μl of the Donor beads the assay plate was incubated for 30 min at room temperature and constant shaking without exposure to direct light. The assay plate was then read on a Paradigm AlphaLisa Reader using the build-in program with excitation at 680 nm and emission at 570 nm.
The measured signals were then used to calculate IC50 values for inhibition of Aβ42 secretion by nonlinear regression fit analysis using XLfit 5.3 software (IDBS).
The table below shows the data for all compounds for the inhibition of Aβ42 secretion (nM):
The compounds of formula I and the pharmaceutically acceptable salts of the compounds of formula I can be used as medicaments, e.g. in the form of pharmaceutical preparations. The pharmaceutical preparations can be administered orally, e.g. in the form of tablets, coated tablets, dragées, hard and soft gelatine capsules, solutions, emulsions or suspensions. The administration can, however, also be effected rectally, e.g. in the form of suppositories, parenterally, e.g. in the form of injection solutions. The administration can also be effected topically, e.g. transdermal administration, or in form of eye drops or ear drops.
The compounds of formula I can be processed with pharmaceutically inert, inorganic or organic carriers for the production of pharmaceutical preparations. Lactose, corn starch or derivatives thereof, talc, stearic acids or its salts and the like can be used, for example, as such carriers for tablets, coated tablets, dragées and hard gelatine capsules. Suitable carriers for soft gelatine capsules are, for example, vegetable oils, waxes, fats, semi-solid and liquid polyols and the like. Depending on the nature of the active substance no carriers are, however, usually required in the case of soft gelatine capsules. Suitable carriers for the production of solutions and syrups are, for example, water, polyols, glycerol, vegetable oil and the like. Suitable carriers for suppositories are, for example, natural or hardened oils, waxes, fats, semi-liquid or liquid polyols and the like.
The pharmaceutical preparations can, moreover, contain preservatives, solubilizers, stabilizers, wetting agents, emulsifiers, sweeteners, colorants, flavorants, salts for varying the osmotic pressure, buffers, masking agents or antioxidants. They can also contain still other therapeutically valuable substances.
Medicaments containing a compound of formula I or a pharmaceutically acceptable salt thereof and a therapeutically inert carrier are also an object of the present invention, as is a process for their production, which comprises bringing one or more compounds of formula I and/or pharmaceutically acceptable acid addition salts and, if desired, one or more other therapeutically valuable substances into a galenical administration form together with one or more therapeutically inert carriers.
In accordance with the invention compounds of formula I as well as their pharmaceutically acceptable salts are useful in the control or prevention of illnesses based on the inhibition of Aβ42 secretion, such as of Alzheimer's disease.
The dosage can vary within wide limits and will, of course, have to be adjusted to the individual requirements in each particular case. In the case of oral administration the dosage for adults can vary from about 0.01 mg to about 1000 mg per day of a compound of general formula I or of the corresponding amount of a pharmaceutically acceptable salt thereof. The daily dosage may be administered as single dose or in divided doses and, in addition, the upper limit can also be exceeded when this is found to be indicated.
The following examples are provided for illustration of the invention. They should not be considered as limiting the scope of the invention, but merely as being representative thereof.
HPLC (method LCMS_fastgradient)
Column: Agilent Zorbax Eclipse Plus C18, Rapid Resolution HT, 2.1×30 mm, 1.8 μm, Part.no. 959731-902
Solvent A: Water 0.01% Formic Acid; Solvent B: acetonitrile (MeCN)
The following abbreviations were used in the experimental part:
Step 1: 3,4-Difluoroaniline (3.00 g, 23.20 mmol) and diphenyl cyanocarbonimidate (5.53 g, 23.20 mmol) were dissolved in 2-propanol (64.00 mL). The reaction mixture was stirred at RT overnight. The crude was evaporated in vacuo and purified by column chromatography (Hept:EtOAc 100:0 to 50:50) to afford (Z)-phenyl N′-cyano-N-(3,4-difluorophenyl) carbamimidate as a white solid (5.00 g, 79%). MS (ES+) m/z: 274.1 [M+H+].
Step 2: A solution of 2-(2-bromoethoxy)tetrahydro-2H-pyran (5.74 g, 4.15 mL, 27.40 mmol), (Z)-phenyl N′-cyano-N-(3,4-difluorophenyl)carbamimidate (5.00 g, 18.30 mmol) and K2CO3 (5.06 g, 36.60 mmol) in DMF (180 mL) was heated at 85° C. overnight. An extra amount of 2-(2-bromoethoxy)tetrahydro-2H-pyran (5.74 g, 4.15 mL, 27.40 mmol) and K2CO3 (5.06 g, 36.60 mmol) were added and the reaction stirred at 100° C. for a further 8 hours and then at 75° C. overnight. The reaction mixture was cooled down to RT, poured onto a saturated aqueous solution of NH4Cl and the product extracted three times with EtOAc. The organic layers were combined, washed with water and then with brine, dried over sodium sulfate, filtered and evaporated in vacuo. The residue was then purified by column chromatography using (Hept:EtOAc 100:0 to 70:30) to afford (Z)-phenyl N′-cyano-N-(3,4-difluorophenyl)-N-(2-((tetrahydro-2H-pyran-2-yl)oxy)ethyl)carbamimidate as a yellow oil (1.75 g, 24%). MS (ES+) m/z: 402.2 [M+H]+.
Step 3: To a solution of (Z)-Phenyl N′-cyano-N-(3,4-difluorophenyl)-N-(2-((tetrahydro-2H-pyran-2-yl)oxy)ethyl) carbamimidate (1.75 g, 4.36 mmol) in methanol (12 mL) was added hydrazine hydrate 25% in H2O (873 mg, 864.00 μl, 4.36 mmol). The reaction mixture was stirred at 35° C. for 1 hour and then evaporated in vacuo to give N3-(3,4-Difluorophenyl)-N3-(2-((tetrahydro-2H-pyran-2-yl)oxy)ethyl)-4H-1,2,4-triazole-3,5-diamine (1.79 g, 91%) which was used directly for the next step without further purification. (1.79 g, 91%). MS (ES+) m/z: 340.2 [M+H]+.
Step 4: The crude of N3-(3,4-difluorophenyl)-N3-(2-((tetrahydro-2H-pyran-2-yl)oxy)ethyl)-4H-1,2,4-triazole-3,5-diamine (1.79 g, 5.27 mmol) was dissolved in a mixture of aqueous HCl 2M (9 mL) an methanol (43 mL). The reaction mixture was stirred at RT for 1 hour and concentrated in vacuo. The residue was diluted with EtOAc and washed with an aqueous NaHCO3 solution. The organic layer was dried Na2SO4 and the product purified by combiflash chromatography (CH2Cl2:MeOH 100:0 to 90:10) to afford 2-((5-Amino-4H-1,2,4-triazol-3-yl)(3,4-difluorophenyl)amino)ethanol as a white solid (0.39 g, 29%). MS (ES+) m/z: 256.2 [M+H]+.
Step 5: To a THF (4 mL) solution of 2-((5-Amino-4H-1,2,4-triazol-3-yl)(3,4-difluorophenyl) amino)ethanol (50.00 mg, 196 μmol) and triphenylphosphine (154 mg, 588 μmol) was added DEAD (70 mg, 64 μl, 392 μmol) the reaction was stirred at RT for 24 hours. The reaction was stirred for further 7 hours at 50° C. and then one extra amount of triphenylphosphine (154 mg, 588 μmol), DEAD (70 mg, 64 μl, 392 μmol) and THF (4 mL) were added. The reaction mixture was left to stir at RT overnight. The mixture was diluted with EtOAc and washed three times with water and brine; the organic phase was dried over sodium sulfate, filtered and evaporated in vacuo. The crude was purified using combiflash chromatography (CH2Cl2:MeOH 100:0 to 90:10) to afford 4-(3,4-difluorophenyl)-5,6-dihydro-4H-imidazo[1,2-b][1,2,4]triazol-2-amine as a white solid (7.2 mg, 15%). MS (ES+) m/z: 238.1 [M+H]+.
Step 6: tert-Butyl nitrile (232 mg, 268 μl, 2.0 mmol) and cupric bromide (452 mg, 96 μl, 2.0 mmol) were combined in CH3CN (40 mL) and stirred at 60° C. before a solution of 4-(3,4-difluorophenyl)-5,6-dihydro-4H-imidazo[1,2-b][1,2,4]triazol-2-amine (140 mg, 590 μmol) in CH3CN (40 mL) was added drop wise. The reaction mixture was stirred at 75° C. for 2 hours, concentrated in vacuo, diluted with 1M HCl and extracted three times with EtOAc. The combined organic layer was dried over sodium sulfate, filtered and evaporated in vacuo. The crude was purified by combiflash chromatography (Hept:EtOAc 100:0 to 50:50) to afford 2-bromo-4-(3,4-difluorophenyl)-5,6-dihydro-4H-imidazo[1,2-b][1,2,4]triazole (0.12 g, 69%) as an off-white solid. MS (ES+) m/z: 301.0 and 303.0 [M+H]+. (Br isotopes).
In analogy to the preparation of the intermediate 9-1, starting from 4-(trifluoromethyl)aniline was prepared 0.12 g of the intermediate 2-bromo-4-[4-(trifluoromethyl)phenyl]-5,6-dihydroimidazo[1,2-b][1,2,4]triazole (9-2) as a white solid. MS (ES+) m/z: 333.0 and 335.0 [M+H+]. (Br isotopes)
In analogy to the preparation of the intermediate 9-1, starting from 2,3,4-trifluoroaniline was prepared 0.12 g of the intermediate 2-bromo-4-(2,3,4-trifluorophenyl)-5,6-dihydroimidazo[1,2-b][1,2,4]triazole (9-3) as a white solid. MS (ES+) m/z: 319.0 and 321.0 [M+H]+. (Br isotopes)
Step 1: 3,4-Difluoroaniline (3.00 g, 23.20 mmol) and diphenyl cyanocarbonimidate (5.53 g, 23.20 mmol) were dissolved in 2-propanol (64.00 mL). The reaction mixture was stirred at RT overnight. The crude was evaporated in vacuo and purified by column chromatography (Hept:EtOAc 100:0 to 50:50) to afford (Z)-phenyl N′-cyano-N-(3,4-difluorophenyl) carbamimidate as a white solid (5.00 g, 79%). MS (ES+) m/z: 274.1 [M+H]+.
Step 2: A solution of (Z)-phenyl N′-cyano-N-(3,4-difluorophenyl)carbamimidate (17.69 g, 64.70 mmol), 2-(3-bromopropoxy)tetrahydro-2H-pyran (22.80 g, 17.10 mL, 97.10 mmol) and K2CO3 (17.90 g, 129.00 mmol) in DMF (200 mL) was heated overnight at 85° C. The reaction mixture was concentrated in vacuo and the residue was purified by column chromatography (Hept:EtOAc 90:10 to 50:50) to give 3-cyano-1-(3,4-difluorophenyl)-2-phenyl-1-(3-tetrahydropyran-2-yloxypropyl)isourea as a white solid (26.90 g, 49%). MS (ES+) m/z: 416.2 [M+H]+.
Step 3: To a solution of of (Z)-phenyl N′-cyano-N-(3,4-difluorophenyl)-N-(3-((tetrahydro-2H-pyran-2-yl)oxy)propyl)carbamimidate (10.20 g, 24.60 mmol) in MeOH (70 mL) was added hydrazine hydrate 25% in water (4.92 g, 4.87 mL, 24.60 mmol). The reaction mixture was stirred at RT overnight, evaporated and purified by column chromatography (CH2Cl2:MeOH 99:1 to 92.5:7.5) to afford N3-(3,4-difluorophenyl)-N3-(3-tetrahydropyran-2-yloxypropyl)-4H-1,2,4-triazole-3,5-diamine as a colourless foam (8.68 g, 78%). MS (ES+) m/z: 354.2 [M+H]+.
Step 4: To a solution of N3-(3,4-difluorophenyl)-N3-(3-((tetrahydro-2H-pyran-2-yl)oxy)propyl)-4H-1,2,4-triazole-3,5-diamine (6.75 g, 19.10 mmol) in MeOH (150 mL) followed by addition of aqueous HCl 2M (30 mL). The reaction mixture was stirred at RT for 90 minutes and concentrated in vacuo. The residue was diluted with EtOAc and washed with an aqueous NaHCO3 solution. The organic layer was dried on Na2SO4 and the product purified by combiflash chromatography to afford 3-(N-(5-Amino-4H-1,2,4-triazol-3-yl)-3,4-difluoro-anilino)propan-1-ol as a white solid (5.14 g, 95%). MS (ES+) m/z: 270.1 [M+H]+.
Step 5: To a solution of 3-((5-amino-4H-1,2,4-triazol-3-yl)(3,4-difluorophenyl)amino) propan-1-ol (4.55 g, 16.90 mmol) in DMF (90 mL) at −15/−20° C., was added triphenylphosphine (6.65 g, 25.30 mmol). The mixture was stirred at −15/−20° C. for 15 minutes, then at −30° C., DEAD (4.55 g, 4.10 ml, 25.30 mmol) was added drop wise over of 20 minutes. The mixture was stirred at −30° C. for 90 minutes. Water was added to the reaction mixture and then concentrated under vacuo, and the product extracted with EtOAc. The combined organic layers were washed once with brine, dried over sodium sulfate, filtered and evaporated in vacuo. The crude was purified using silica chromatography (CH2Cl2:MeOH 99:1 to 96:4) to afford 4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidin-2-amine as a white solid (3.41 g, 80%). MS (ES+) m/z: 252.1 [M+H]+.
Step 6: To a black solution of tert-butyl nitrite (1.01 g, 1.17 mL, 8.86 mmol) and cupric bromide (1.98 g, 420 μl, 8.86 mmol) in CH3CN (150 mL) at 60° C., 4-(3,4-difluorophenyl)-4,5,6,7-tetrahydro-[1,2,4]triazolo[1,5-a]pyrimidin-2-amine (1.63 g, 5.90 mmol,) in 50 mL CH3CN was added drop wise. After addition, the reaction mixture was heated to 75° C. and stirred for 30 minutes. The reaction mixture was concentrated in vacuo, diluted with 2 mL of 1M HCl and extracted 3 times with EtOAc. The organic layers were dried over sodium sulfate and concentrated. The crude was purified by chromatography (CH2Cl2:MeOH 99.5:0.5) to afford 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine as a light yellow solid (1.86 g, 82%). MS (ES+) m/z: 315.0 and 317.0 [M+H]+. (Br isotopes).
In analogy to the preparation of the intermediate 15-1, starting from 3,5-difluoroaniline was prepared 234 mg of the intermediate 2-bromo-4-(3,5-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-2) as a white solid. MS (ES+) m/z: 315.0 and 317.0 [M+H]+. (Br isotopes).
In analogy to the preparation of the intermediate 15-1, starting from 3-fluoro-4-methyl-aniline was prepared 662 mg of the intermediate 2-bromo-4-(3-fluoro-4-methyl-phenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-3) as a light yellow oil. MS (ES+) m/z: 311.0 and 313.0 [M+H]+. (Br isotopes).
In analogy to the preparation of the intermediate 15-1, starting from 2,3,4-trifluoroaniline was prepared 134 mg of the intermediate 2-bromo-4-(2,3,4-trifluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-4) as a light brown solid. MS (ES+) m/z: 333.0 and 335.0 [M+H]+. (Br isotopes).
In analogy to the preparation of the intermediate 15-1, starting from 3-fluoroaniline was prepared 210 mg of the intermediate 2-bromo-4-(3-fluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-5) as a light yellow solid. MS (ES+) m/z: 297.0 and 299.0 [M+H]+. (Br isotopes).
In analogy to the preparation of the intermediate 15-1, starting from 4-fluoroaniline was prepared 1.11 g of the intermediate 2-bromo-4-(4-fluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-6) as a light yellow solid. MS (ES+) m/z: 297.0 and 299.0 [M+H]+. (Br isotopes).
In analogy to the preparation of the intermediate 15-1, starting from aniline was prepared 240 mg of the intermediate 2-bromo-4-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-7) as a white solid. MS (ES+) m/z: 279.0 and 281.0 [M+H]+. (Br isotopes).
Step 1: 3,4-Difluoroaniline (3.00 g, 23.20 mmol) and diphenyl cyanocarbonimidate (5.53 g, 23.20 mmol) were dissolved in 2-propanol (64.00 mL). The reaction mixture was stirred at RT overnight. The crude was evaporated in vacuo and purified by column chromatography (Hept:EtOAc 100:0 to 50:50) to afford (Z)-phenyl N′-cyano-N-(3,4-difluorophenyl) carbamimidate as a white solid (5.00 g, 79%). MS (ES+) m/z: 274.1 [M+H]+.
Step 2: A solution of (Z)-phenyl N′-cyano-N-(3,4-difluorophenyl)carbamimidate (10.0 g, 36.6 mmol), 2-(3-bromobutoxy)tetrahydro-2H-pyran (13.30 g, 54.90 mmol) and K2CO3 (10.10 g, 73.20 mmol) in DMF (350 mL) was heated overnight at 85° C. The reaction mixture was concentrated in vacuo and the residue was purified by column chromatography (Hept:EtOAc 90:10 to 50:50) to give 3-cyano-1-(3,4-difluorophenyl)-2-phenyl-1-(4-tetrahydropyran-2-yloxybutyl)isourea as a colorless oil (5.31 g, 34%). MS (ES+) m/z: 430.2 [M+H]+.
Step 3: To a solution of 3-cyano-1-(3,4-difluorophenyl)-2-phenyl-1-(4-tetrahydropyran-2-yloxybutyl)isourea (5.29 g, 12.30 mmol) in MeOH (40 mL) was added hydrazine hydrate 25% in water (2.47 g, 2.44 mL, 12.30 mmol). The reaction mixture was stirred at RT for 7 hours, evaporated and purified by column chromatography (CH2Cl2:MeOH 99:1 to 92.5:7.5) to afford N3-(3,4-difluorophenyl)-N3-(3-tetrahydropyran-2-yloxybutyl)-4H-1,2,4-triazole-3,5-diamine as a colourless oil (3.54 g, 78%). MS (ES+) m/z: 368.2 [M+H]+.
Step 4: To a solution of N3-(3,4-difluorophenyl)-N3-(3-tetrahydropyran-2-yloxybutyl)-4H-1,2,4-triazole-3,5-diamine (3.50 g, 9.53 mmol) in MeOH (85 mL) was added an aqueous HCl 2M solution (17 mL). The reaction mixture was stirred at RT for 90 minutes and concentrated in vacuo. The residue was diluted with EtOAc and washed with an aqueous NaHCO3 solution. The organic layer was dried on Na2SO4 and the product purified by combiflash chromatography to afford 4-(N-(5-amino-4H-1,2,4-triazol-3-yl)-3,4-difluoro-anilino)butan-1-ol as a white solid (2.51 g, 93%). MS (ES+) m/z: 284.1 [M+H]+.
Step 5: At 0° C., cyanomethylenetrimethylphosphorane 0.5 M in THF (8.47 mL, 4.24 mmol) was added drop wise within 15 minutes to a solution of 4-((5-amino-4H-1,2,4-triazol-3-yl)(3,4-difluorophenyl)amino)butan-1-ol (1.00 g, 3.53 mmol) in THF (120 mL) at 0° C. and stirred at RT overnight. The mixture was concentrated under vacuo, and the crude diluted with EtOAc and washed with water. The organic layer was dried over sodium sulfate, filtered and evaporated in vacuo. The crude was purified using silica chromatography (CH2Cl2:MeOH 99:1 to 96:4) to afford 4-(3,4-difluorophenyl)-5,6,7,8-tetrahydro-[1,2,4]triazolo[1,5-a][1,3]diazepin-2-amine as a white solid (0.2 g, 21%). MS (ES+) m/z: 266.1 [M+H]+.
Step 6: To a black solution of tert-butyl nitrite (115 mg, 0.13 mL, 1.01 mmol) and cupric bromide (225 mg, 47.7 μl, 1.01 mmol) in CH3CN (15 mL) at 60° C., 4-(3,4-difluorophenyl)-5,6,7,8-tetrahydro-[1,2,4]triazolo[1,5-a][1,3]diazepin-2-amine (178 mg, 0.671 mmol,) in 25 mL CH3CN was added drop wise. After addition, the reaction mixture was heated to 75° C. and stirred for 30 minutes. The reaction mixture was concentrated in vacuo, diluted with 2 mL of 1M HCl and extracted 3 times with EtOAc. The organic layers were dried over sodium sulfate and concentrated. The crude was purified by chromatography (CH2Cl2:MeOH 99.5:0.5) to afford 2-bromo-4-(3,4-difluorophenyl)-5,6,7,8-tetrahydro-[1,2,4]triazolo[1,5-a][1,3]diazepine as a light yellow oil (0.13 g, 58%). MS (ES+) m/z: 329.0 and 331.0 [M+H]+. (Br isotopes).
To a solution of an intermediate 8, 9, 14, 15, 19 or 20 in 1,4-dioxane was added 1.5 equivalent of an intermediate 10. The reaction mixture was degased and a Pd(OAc)2 (0.2 eq.), Xantphos (0.25 eq.) and cesium carbonate (2.0 eq.) were added. The reaction mixture was heated at 100° C. until completion of the reaction (usually between 0.5 and 8 hours) and concentrated under vacuo. A purification was done either by column chromatography or reverse phase preparative HPLC to afford the desired product.
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 3-(difluoromethoxy)-4-(3-methyl-1,2,4-triazol-1-yl)aniline was prepared 80 mg of the title compound as a light yellow solid. MS (ES+) m/z: 475.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-51-1-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 3-methoxy-4-(3-methyl-1,2,4-triazol-1-yl)aniline was prepared 61 mg of the title compound as a light yellow solid. MS (ES+) m/z: 439.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 3-methoxy-4-[4-(trifluoromethyl)imidazol-1-yl] aniline was prepared 62 mg of the title compound as a white solid. MS (ES+) m/z: 492.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 3-methoxy-4-(2-methyloxazol-5-yl)aniline was prepared 35 mg of the title compound as a light yellow solid. MS (ES+) m/z: 438.4 [(M+H)+].
Using the general procedure 1, from 4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidin-2-amine (described as precursor of 15-1) and 5-bromo-2-(4-chloroimidazol-1-yl)-3-methoxy-pyridine was prepared 16 mg of the title compound as a white solid. MS (ES+) m/z: 459.1 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 4-(4-chloroimidazol-1-yl)-3-methoxy-aniline was prepared 10 mg of the title compound as a white solid. MS (ES+) m/z: 458.1 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-5,6,7,8-tetrahydro-[1,2,4]triazolo[1,5-a][1,3]diazepine (20-1) and 3-methoxy-4-(2-methyloxazol-5-yl)aniline was prepared 7.5 mg of the title compound as a white solid. MS (ES+) m/z: 453.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-5,6,7,8-tetrahydro-[1,2,4]triazolo[1,5-a][1,3]diazepine (20-1) and 3-methoxy-4-(3-methyl-1,2,4-triazol-1-yl)aniline was prepared 11 mg of the title compound as a white solid. MS (ES+) m/z: 453.3 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-5,6,7,8-tetrahydro-[1,2,4]triazolo[1,5-a][1,3]diazepine (20-1) and 4-(4-chloroimidazol-1-yl)-3-methoxy-aniline was prepared 7 mg of the title compound as a white solid. MS (ES+) m/z: 472.1 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-5,6,7,8-tetrahydro-[1,2,4]triazolo[1,5-a][1,3]diazepine (20-1) and 3-methoxy-4-[4-(trifluoromethyl)imidazol-1-yl] aniline was prepared 10 mg of the title compound as a white solid. MS (ES+) m/z: 505.4 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 3-fluoro-4-(3-methyl-1,2,4-triazol-1-yl)aniline was prepared 16 mg of the title compound as a white solid. MS (ES+) m/z: 427.3 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 4-(3-chloro-1,2,4-triazol-1-yl)-3-methoxy-aniline was prepared 9 mg of the title compound as a white solid. MS (ES+) m/z: 459.1 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo [1,5-a]pyrimidine (15-1) and 3-fluoro-4-(5-methyl-1,2,4-triazol-1-yl)aniline was prepared 6 mg of the title compound as a white solid. MS (ES+) m/z: 427.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 3-fluoro-4-(1,2,4-triazol-1-yl)aniline was prepared 4 mg of the title compound as a white solid. MS (ES+) m/z: 413.1 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(2,3,4-trifluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-4) and 3-methoxy-4-(2-methyloxazol-5-yl)aniline was prepared 10 mg of the title compound as a white solid. MS (ES+) m/z: 457.3 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(2,3,4-trifluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-4) and 3-methoxy-4-(3-methyl-1,2,4-triazol-1-yl)aniline was prepared 10 mg of the title compound as a white solid. MS (ES+) m/z: 457.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(2,3,4-trifluorophenyl)-6,7-dihydro-51-1-[1,2,4]triazolo[1,5-a]pyrimidine (15-4) and 4-(4-chloroimidazol-1-yl)-3-methoxy-aniline was prepared 7 mg of the title compound as a white solid. MS (ES+) m/z: 476.1 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 4-(4-chloroimidazol-1-yl)-3-fluoro-aniline was prepared 11 mg of the title compound as a white solid. MS (ES+) m/z: 446.1 [(M+H)+].
Using the general procedure 1, from 4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidin-2-amine (precursor of 15-1) and 5-(4-bromo-2-fluoro-phenyl)-2-methyl-oxazole was prepared 16 mg of the title compound as a white solid. MS (ES+) m/z: 427.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 3-methoxy-4-(1-methylpyrazol-4-yl)aniline was prepared 11 mg of the title compound as a white solid. MS (ES+) m/z: 438.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 4-(3-chloro-1,2,4-triazol-1-yl)-3-fluoro-aniline was prepared 6 mg of the title compound as a white solid. MS (ES+) m/z: 447.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 4-(2-methyloxazol-5-yl)aniline was prepared 6 mg of the title compound as a white solid. MS (ES+) m/z: 409.2 [(M+H)+].
Using the general procedure 1, from 4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidin-2-amine (precursor of 15-1) and 1-(4-bromophenyl)-4-chloro-imidazole was prepared 12 mg of the title compound as a white solid. MS (ES+) m/z: 428.2 [(M+H)+].
Using the general procedure 1, from 4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidin-2-amine (precursor of 15-1) and 5-bromo-2-(4-chloroimidazol-1-yl)benzonitrile was prepared 20 mg of the title compound as a white solid. MS (ES+) m/z: 453.2 [(M+H)+].
Using the general procedure 1, from 4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidin-2-amine (precursor of 15-1) and 5-(4-bromo-2-methoxy-phenyl)-3-methyl-1H-pyrazole was prepared 11 mg of the title compound as a white solid. MS (ES+) m/z: 438.3 [(M+H)+].
Using the general procedure 1, from 4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidin-2-amine (precursor of 15-1) and 5-bromo-2-[4-(difluoromethyl)imidazol-1-yl]benzonitrile was prepared 25 mg of the title compound as a white solid. MS (ES+) m/z: 469.2 [(M+H)+].
Using the general procedure 1, from 4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidin-2-amine (precursor of 15-1) and 5-bromo-2-(2-methyloxazol-5-yl)benzonitrile was prepared 4 mg of the title compound as a white solid. MS (ES+) m/z: 434.2 [(M+H)+].
Using the general procedure 1, from 4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidin-2-amine (precursor of 15-1) and 1-(4-bromo-2-chloro-phenyl)-4-chloro-imidazole was prepared 23 mg of the title compound as a white solid. MS (ES+) m/z: 462.3 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 4-(3-methyl-1,2,4-triazol-1-yl)aniline was prepared 8 mg of the title compound as a white solid. MS (ES+) m/z: 409.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 5-amino-2-(3-methyl-1,2,4-triazol-1-yl)benzonitrile was prepared 10 mg of the title compound as a white solid. MS (ES+) m/z: 434.3 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3-fluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-5) and 3-methoxy-4-(3-methyl-1,2,4-triazol-1-yl)aniline was prepared 23 mg of the title compound as a white solid. MS (ES+) m/z: 421.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3-fluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-5) and 4-(4-chloroimidazol-1-yl)-3-methoxy-aniline was prepared 29 mg of the title compound as a white solid. MS (ES+) m/z: 440.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(4-fluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-6) and 3-methoxy-4-(3-methyl-1,2,4-triazol-1-yl)aniline was prepared 21 mg of the title compound as a white solid. MS (ES+) m/z: 421.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(4-fluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-6) and 3-methoxy-4-(3-methyl-1,2,4-triazol-1-yl)aniline was prepared 21 mg of the title compound as a white solid. MS (ES+) m/z: 421.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(4-fluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-6) and 4-(4-chloroimidazol-1-yl)-3-methoxy-aniline was prepared 34 mg of the title compound as a white solid. MS (ES+) m/z: 440.1 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(4-fluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (precursor of 15-6) and 5-bromo-2-(4-chloroimidazol-1-yl)benzonitrile was prepared 36 mg of the title compound as a white solid. MS (ES+) m/z: 435.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(4-fluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-6) and 4-(4-chloroimidazol-1-yl)-3-fluoro-aniline was prepared 36 mg of the title compound as a white solid. MS (ES+) m/z: 428.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(4-fluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-6) and 3-(difluoromethoxy)-4-(3-methyl-1,2,4-triazol-1-yl)aniline was prepared 30 mg of the title compound as a white solid. MS (ES+) m/z: 457.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(4-fluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-6) and 6-(4-chloroimidazol-1-yl)-5-methoxy-pyridin-3-amine was prepared 23 mg of the title compound as a white solid. MS (ES+) m/z: 441.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(4-fluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (precursor of 15-6) and 5-(5-bromo-3-fluoro-2-pyridyl)-2-methyl-oxazole was prepared 15 mg of the title compound as a white solid. MS (ES+) m/z: 409.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 4-(4-fluoroimidazol-1-yl)-3-methoxy-aniline was prepared 24 mg of the title compound as a white solid. MS (ES+) m/z: 442.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,5-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-2) and 3-methoxy-4-(3-methyl-1,2,4-triazol-1-yl)aniline was prepared 16 mg of the title compound as a white solid. MS (ES+) m/z: 439.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,5-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-2) and 4-(4-chloroimidazol-1-yl)-3-methoxy-aniline was prepared 15 mg of the title compound as a white solid. MS (ES+) m/z: 458.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(4-fluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-6) and 3-methoxy-4-(2-methyloxazol-5-yl)aniline was prepared 13 mg of the title compound as a white solid. MS (ES+) m/z: 421.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-[4-(trifluoromethyl)phenyl]-5,6-dihydroimidazo[1,2-b][1,2,4]triazole (9-2) and 4-(4-chloroimidazol-1-yl)-3-methoxy-aniline was prepared 17 mg of the title compound as a white solid. MS (ES+) m/z: 476.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(2,3,4-trifluorophenyl)-5,6-dihydroimidazo[1,2-b][1,2,4]triazole (9-3) and 4-(4-chloroimidazol-1-yl)-3-methoxy-aniline was prepared 32 mg of the title compound as a white solid. MS (ES+) m/z: 462.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-5,6-dihydroimidazo[1,2-b][1,2,4]triazole (9-1) and 4-(4-chloroimidazol-1-yl)-3-methoxy-aniline was prepared 11 mg of the title compound as a white solid. MS (ES+) m/z: 444.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-(3,4-difluorophenyl)-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-1) and 4-(4-methyltriazol-1-yl)aniline was prepared 20 mg of the title compound as a white solid. MS (ES+) m/z: 409.2 [(M+H)+].
Using the general procedure 1, from 2-bromo-4-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[1,5-a]pyrimidine (15-7) and 3-methoxy-4-(3-methyl-1,2,4-triazol-1-yl)aniline was prepared 11 mg of the title compound as a white solid. MS (ES+) m/z: 403.1 [(M+H)+].
| Number | Date | Country | Kind |
|---|---|---|---|
| 16192237.2 | Oct 2016 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2017/074927 | 10/2/2017 | WO | 00 |
