Biguanide derivatives, manufacturing method thereof, and disinfectants containing the derivatives

Abstract

The invention presents a biguanide derivative or its salt expressed by a formula: ##STR1## (where R.sup.1 and R.sup.2 are as defined in Specification), or formula: ##STR2## (where A and R.sup.3 is as defined in specification). This biguanide derivative or its salt is preferably used as the effective amount of a disinfectant for humans, animals, medical appliances, etc.

Description

BACKGROUND OF THE INVENTION

The invention relates to novel biguanide derivatives used in disinfection of humans, animals, medical appliances, and others, a method of manufacturing the same, and disinfectants containing such derivatives.

It is known that various guanidine derivatives possess bactericidal actions, and biguanide derivatives of 1,4-dimethylene cyclohexane type are disclosed in the British Patent Specification No. 1,098,902.

SUMMARY OF THE INVENTION

It is a primary object of this invention to present novel biguanide derivatives or their salts possessing a higher activity than the conventional biguanide derivatives or their salts, and disinfectants containing such derivatives or their salts.

To achieve the above object, a monobiguanide derivative of the invention is a biguanide derivative or its salt, expressed by a general formula: ##STR3## (where R.sup.1 represents 3,4-dichlorobenzyl group, 4-chlorophenyl group, 3,4-dichlorophenyl group, benzyl group or 4-chlorobenzyl group, and R.sup.2 represents octyl group, 3,4-dichlorobenzyl group, dodecyl group, decyl group, 3-trifluoromethylphenyl group, 4-bromophenyl group, 4-iodophenyl group, 2,4-dichlorophenyl group, 3,4-dichlorophenyl group, 2,3,4-trichlorophenyl group, 3,4-dimethylphenyl group, 3,4-methylenedioxyphenyl group, 4-t-butylphenyl group, 4-ethylthiophenyl group, 1,1,3,3-tetramethylbutyl group, hexyl group, 2-ethoxyethyl group, 2-(2-hydroxyethoxy)ethyl group, 3-diethylaminopropyl group, 3-(2-ethylhexyloxy)-propyl group, (3-isopropoxy)propyl group, (2-diethylamino)-ethyl group, (3-butyl)-propyl group, 3(di-n-butylamino)propyl group, cyclohexylmethyl group, 3-trifluoromethylphenyl group, 4-ethylthiophenyl group, 4-chlorobenzyl group, 2,4-dichlorobenzyl group, 4-acetylaminophenyl group, 3,4-methylenedioxyphenyl group, 3,4-methylenedioxybenzyl group, octyl group, 4-chlorobenzyl group, decyl group, dodecyl group, isobutyl group, 3,4-dichlorophenyl group, or hexyl group), and the derivative being N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide,

N.sup.1 -(4-chlorophenyl)-N.sup.5 -(3,4-dichlorobenzyl)-biguanide,

N.sup.1 -(3,4-dichlorophenyl)-N.sup.5 -octyl-biguanide,

N.sup.1 -benzyl-N.sup.5 -dodecyl-biguanide,

N.sup.1 -benzyl-N.sup.5 -decyl-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(3-trifluoromethylphenyl)-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(4-bromophenyl)-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(4-iodophenyl)-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(2,4-dichlorophenyl)-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(3,4-dichlorophenyl)-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(2,3,4-trichlorophenyl)-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(3,4-dimethylphenyl)-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(3,4-methylenedioxyphenyl)-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(4-t-butylphenyl)-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(4-ethylthiophenyl)-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(n-decyl)-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(1,1,3,3-tetramethylbutyl)-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -hexyl-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(2-ethoxyethyl)-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -[2-(2-hydroxyethoxy) ethyl]-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(3-diethylaminopropyl)-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -[3-(2-ethylhexyloxy)propyl]-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -[(3-isopropoxy)propyl]-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -[(2-diethylamino)-ethyl]-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -[(3-butyl)-propyl]-biguanide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -[3(di-n-butylamino)propyl]-biguanide,

N.sup.1 -(4-chlorophenyl)-N.sup.5 -cyclohexylmethyl-biguanide,

N.sup.1 -(4-chlorophenyl)-N.sup.5 -(3-trifluoromethylphenyl)-biguanide,

N.sup.1 -(4-chlorophenyl)-N.sup.5 -(4-ethylthiophenyl)-biguanide,

N.sup.1 -(4-chlorophenyl)-N.sup.5 -(4-chlorobenzyl)-biguanide,

N.sup.1 -(4-chlorophenyl)-N.sup.5 -(2,4-dichlorobenzyl)-biguanide,

N.sup.1 -(4-chlorophenyl)-N.sup.5 -(4-acetylaminophenyl)-biguanide,

N.sup.1 -(4-chlorophenyl)-N.sup.5 -(3,4-methylenedioxyphenyl)-biguanide,

N.sup.1 -(4-chlorophenyl)-N.sup.5 -(3,4-methyleneoxydibenzyl)-biguanide,

N.sup.1 -(4-chlorobenzyl)-N.sup.5 -octyl-biguanide,

N.sup.1 -(4-chlorobenzyl)-N.sup.5 -(4-chlorobenzyl)-biguanide,

N.sup.1 -(4-chlorobenzyl)-N.sup.5 -decyl-biguanide,

N.sup.1 -(4-chlorobenzyl)-N.sup.5 -dodecyl-biguanide,

N.sup.1 -(4-chlorobenzyl)-N.sup.5 -isobutyl-biguanide,

N.sup.1 -(4-chlorobenzyl)-N.sup.5 -(3,4-dichlorophenyl)-biguanide,

N.sup.1 -(3,4-dichlorophenyl)-N.sup.5 -(3,4-methylenedioxybenzyl)-biguanide,

N.sup.1 -(3,4-dichlorophenyl)-N.sup.5 -hexyl-biguanide,

N.sup.1 -(3,4-dichlorophenyl)-N.sup.5 -decyl-biguanide.

The biguanide derivative (1) or its salt of the invention possesses a higher bactericidal action or anti-bacterial action than a known biguanide. Therefore, the disinfectant of the invention contains the biguanide derivative expressed by Formula (1) or its salt as an active ingredient.

This invention also presents a novel bis-biguanide derivative or its salt expressed by a general formula (2): ##STR4## (where R.sup.3 represents phenyl group which may posess one to three groups selected from a group consisting of halogen atom, lower alkyl group, halogen substituted lower alkyl group and lower alkylthio group as substituent; phenyl lower alkyl group which may possess a halogen atom as substituent on phenyl ring; or alkyl group, and A denotes a lower alkylene group).

The biguanide derivative (2) or its salt also possesses a high bactericidal action or antibacterial action same as the biguanide derivative (1). Therefore, the other disinifectant of this invention contains the bis-biguanide derivative expressed by Formula (2) or its salt as an active ingredient.

In the following explanation, the compound expressed by the general formula (1) is called as a monobiguanide derivative, and the compound expressed in Formula (2) is called a bis-biguanide derivative.

DETAILED DESCRIPTION OF THE INVENTION

The monobiguanide derivative expressed by Formula (1) or its salt of the invention is specifically represented in the Examples hereinafter.

The monobiguanide derivative (1) of the invention is a basic compound, and forms a corresponding acid-addition salt by reacting with an organic acid or an inorganic acid. Acids for forming such salt may include, for example, formic acid, acetic acid, lactic acid, butyric acid, isobutyric acid, .alpha.-mercaptopropionic acid, trifluoroacetic acid, malic acid, fumaric acid, succinic acid, succinic monoamide, glutamic acid, tartaric acid, oxalic acid, citric acid, glycolic acid, gluconic acid, saccharic acid, ascorbic acid, penicillin, benzoic acid, phthalic acid, salicylic acid, anthranilic acid, benzenesulfonic acid, p-toluenesulfonic acid, methansulfonic acid, hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid and carbonic acid, and are not particularly limited. Besides, the ratio of monobiguanide derivative and acid for forming acid-addition salt is not particularly limited, and salts of various ratios may be used such as 1:1, 1:2, or the like.

The acid-addition salt is manufactured in an ordinary salt forming method, such as direct mixing of acid and base, dissolving one or both in solvent such as water and mixing, and charging acid or base in solvent to dissolve and mix. After reaction, the obtained acid-addition salt is filtered if insoluble or hardly soluble in reaction medium, or recovered by evaporating the reaction medium if soluble in the reaction medium.

Such acid-addition salt itself exhibits a high bactericidal effect or antibacterial effect, and is also useful for refining or isolation of free base. Meanwhile, the acid-addition salt is preferred to be acceptable pharmaceutically.

The monobiguanide derivative (1) of this invention may also form a stable coordination compound with a metal salt. Such coordination compound also presents a bactericidal effect. The coordination compound is obtained by reaction of its derivative or its acid-adition salt with a specified quantity of metal salt, preferably a heavy metal salt such as V, Cr, Mn, Co, Ni, Cu, Zn, Pd, Re and Os.

Next is explained a manufacturing method of the monobiguanide derivative (1) of the invention. ##STR5## (where R.sup.1 and R.sup.2 are same as defined above.)

As shown in the reaction formula above, the monobiguanide derivative (1) of the invention is obtained by reaction of the amine expressed by Formula (a) with N.sup.1 -cyanoguanidine compound expressed by Formula (b). At this time, the amine (a) is used in about equimolecular amounts of the compound (b). The amine (a) may be used in a form of proper acid-addition salt such as hydrochloric acid salt.

The both components (a) and (b) are caused to react by heating in the presence or absence of inert solvent, such as 2-ethoxyethanol, 2-methoxyethanol and o-dichlorobenzene. If the product is an acid-addition salt, it may be changed into a form of free base by causing an alkali such as sodium hydroxide to act thereon, or may be changed into other acid-addition salt by ion exchange or other process.

The starting material expressed by Formula (b) may be manufactured, for example, in the following reaction formula. ##STR6## (where M represents an alkali metal, and R.sup.2 is same as defined above.)

That is, the amine expressed by Formula (c) or its acid-addition salt (hydrochloride, etc.) is caused to react with the alkali metal salt (d) of dicyanamide (for example, sodium dicyanamide, potassium dicyanamide) in an inert solvent to obtain the material.

The monobiguanide derivative (1) of this invention may be also manufactured in the following reaction formula. ##STR7## (where R.sup.1 and R.sup.2 represent the same as above.)

This reaction is performed nearly in the same condition as in the above reaction.

The bis-biguanide derivative (2) of this invention is described below. Examples of halogen atom represented by R.sup.3 in Formula (3) may include chlorine, bromine, iodine and fluorine.

Examples of lower alkyl group include straight-chain or branched alkyl group having 1 to 6 carbon atoms, such as methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, n-amyl, n-hexyl group, and the like.

Examples of halogen substituted lower alkyl group include halogen substitued lower alkyl group having 1 to 6 carbon atoms and replaced by 1 to 3 halogen atoms, such as monochloromethyl, monobromomethyl, monofluoromethyl, monoiodomethyl, dichloromethyl, dibromomethyl, difluoromethyl, diiodomethyl, trichloromethyl, tribromomethyl, trifluoromethyl, triiodomethyl, 3-chloropropyl, 3-fluoropropyl, 2,3-dichloropropyl, 3,3,3-trichloropropyl, 3-chloro-2-methylpropyl, 4-chlorobutyl, 4-fluorobutyl, 5-chloropentyl, 6-chlorohexyl, 6-fluorohexyl group, and the like.

Examples of lower alkylthio group include lower alkylthio group having straight-chain or branched alkyl moiety having 1 to 6 carbon atoms, such as methylthio, ethylthio, n-propylthio, isopropylthio, n-butylthio, isobutylthio, tert-butylthio, n-amylthio, n-hexylthio group, and the like.

Examples of phenyl lower alkyl group which may possess a halogen atom on a phenyl group as a substituent may include phenyl lower alkyl group possessing one to three phenyl groups which may have one to three halogen atoms, and having 1 to 6 carbon atoms in the alkyl moiety, such as benzyl, .alpha.-phenethyl, .beta.-phenethyl, 3-phenylpropyl group, benzhydryl, trithyl, 4-chlorophenylmethyl, 3-chlorophenylmethyl, 2-chlorophenylmethyl, 3,4-dichlorophenylmethyl, 4-fluorophenylmethyl, 3,4-difluorophenylmethyl, 4-chlorophenylethyl, 3,4-dichlorophenylethyl group, and the like.

Examples of alkyl group used as subsituent R.sup.3 are straight-chain or branched alkyl groups having 6 to 16 carbon atoms, specifically including n-hexyl, n-heptyl, n-octyl, n-nonyl, n-decyl, n-undecyl, n-tetradecyl, n-hexadecyl, 2-ethyl-1-hexyl, 2-ethyl-1-heptyl, 2-heptyl, 2-octyl, and 1,1,3,3-tetramethylbutyl group.

Examples of lower alkylene group include alkylene group having 1 to 6 carbon atoms, such as methylene, methylmethylene, ethylene, dimethylmethylene, trimethylene, 1-methyl-1-trimethyl, 2-methyltrimethylene, 2,2-dimethyltrimethylene, tetramethylene, pentamethylene, hexamethylene, and the like.

Practical examples of the bis-biguanide derivative (2) of the invention are shown in the table below. ##STR8##

______________________________________R.sup.3 A______________________________________ CH.sub.2 ##STR9## " ##STR10## " ##STR11## " ##STR12## " ##STR13## " ##STR14## " ##STR15## " ##STR16## " ##STR17## " ##STR18## CH.sub.2 ##STR19## " ##STR20## " ##STR21## CH.sub.2 CH.sub.2 ##STR22## " ##STR23## CH.sub.2 ##STR24## " ##STR25## " ##STR26## (CH.sub.2 ) .sub.3H.sub.3 C CH.sub.2(H.sub.3 C).sub.3 C "______________________________________

The bis-biguanide derivative (2) of this invention is a basic compound, and forms, same as the monobiguanide derivative (1), a corresponding acid-addition salt by reaction with various organic acids or inorganic acids.

The acid-addition salt of the bis-biguanide derivative (2) itself, as well as its derivative (2), exhibits a high bactericidal effect or antibacterial effect, and it is also useful for refining or isolation of free base. The acid-addition salt is preferred to be acceptable pharmaceutically. The bis-biguanide derivative (2) of the invention, same as the monobiguanide derivative (1), may also form a stable coordination compound with a metal salt, and such coordination compound also presents an excellent bactericidal effect.

The bis-biguanide derivative (2) of the invention is manufactured, for example, in the following reaction formula. ##STR27## (where A and R.sup.3 represent same as defined above.)

As shown in the reaction formula above, the bis-biguanide derivative (2) of this invention is obtained by the reaction of 1,1'-[1,3-cyclohexanebis(alkylene)]-bis(3-cyanoguanidine) shown in Formula (e) with the amine shown in Formula (f). At this time, the amine (f) is used by about twice the tool equivalent of the compound (e). The amine (f) may be in a form of proper acid-addition salt such as hydrochloride.

The both components (e) and (f) are caused to react with each other by heating in the presence or absence of an inert solvent (e.g. 2-ethoxyethanol, 2-methoxyethanol, o-dichlorobenzene). If the reaction product is an acid-addition salt, it may be changed into a form of a free base by causing an alkali such as sodium hydroxide to act thereon, or changed into other acid-addition salt by ion exchange or other process.

The starting material expressed by Formula (e) may be manufactured, for example, in the following reaction formula. ##STR28## (where M denotes an alkali metal, and A is same as defined above.)

It is obtained, accordingly, by reaction of the diamine shown in Formula (g) or its acid-addition salt (hydrochloride, etc.) with the alkali metal salt (h) of dicyanamide (e.g. sodium dicyanamide, potassium dicyanamide) in an inert solvent.

The bis-biguanide derivative (2) of this invention may be also manufactured in the following reaction formula. ##STR29## (where A and R.sup.3 represent the same as above.)

As shown in the above reaction formula, the bis-biguanide derivative (2) is obtained by the reaction between the diamine expressed by Formula (i) or its acid-addition salt (hydrochloride, etc.) and the cyanoguanidine compound expressed by Formula (j), in the presence or absence of an inert solvent. The reaction is conducted under heating. The cyanoguanidine compound (j) is used at a rate of about twice the mol equivalent of the diamine (i).

The biguanide derivatives (1), (2) of this invention and their salts possess higher antibacterial activities (bactericidal action or antibacterial action) as compared with the hitherto known biguanide derivatives, and some of them also possess antiviral activity. Furthermore, the biguanide derivatives (1), (2) of the invention and their salts possess a broad antibacterial spectrum, and are also excellent in rapidity of action, stability, safety and others, and their activities last for a long time. In addition, they are stimulation-free, odor-free, and soluble also in water. In particular, the compounds in Examples 1 to 4 mentioned below and their salts are particularly excellent in these features, and above all the compound of Example 1 and its salt are excellent in, including the above features, broad antibacterial spectrum, high short-time bactericidal activity, long-lasting drug effect, no problem in color, odor and taste, low toxicity, and the like. The salt of the compound of this invention is excellent in various points, including the above features, such as high solubility, low stimulation and toxicity, high stability, and the like.

Therefore, the biguanide derivatives (1), (2) of this invention and their salts are useful as active ingredient for disinfectants for humans, animals, and medical appliances. The disinfectant of this invention is used in a form of solution, dispersion or suspension by dissolving, dispersing or suspending a specified amount of the biguanide derivatives (1), (2) or their salts in water or organic solvent. Typical examples are eye-drop, nose-drop, gargle, detergent, cleaning agent, and other external application liquids. In these cases, the content of the biguanide derivatives (1), (2) or their salts may be usually about 0.01 to 20% by weight of the total amount.

Besides, the biguanide derivatives (1), (2) of this invention and their salts may be contained in various cosmetics, such as creams, lotions, powders, colors, makeups, toothpaste, shampoo, soap, depilatories, bleaches, hair-dyes, hair tonics, bath additives, manicure, antiperspiration agent, deodorant, aerosol cosmetics, and baby cosmetics, and the like.

Cosmetics are manufactured by dissolving, dispersing or suspending the specified amount of the biguanide derivatives (1), (2) or their salts, together with other ingredients, in water, other solvent, or various cosmetic bases. The content of the biguanide derivative (1), (2) or their salts in the cosmetic is usually about 0.001 to 1% by weight of the total amount.

EXAMPLES

Referring to examples, the biguanide derivative of this invention and the disinifectant containing the derivative are explained in detail below. It must be, however, noted that this invention is not limited to these examples.

EXAMPLE 1

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide hydrochloride

To 20 g of N.sup.1 -cyano-N.sup.3 -octyl-guanidine and 20.2 g of 3,4-dichlorobenzylamine hydrochloride, 200 ml of mesitylene was added, which was heated and refluxed for 1.5 hours. Returning to room temperature after reaction, mesitylene was removed. To the residue, 200 ml of 10% ethanol aqueous solution was added, which was heated, and stirred for 3 hours at room temperature to be solidified. It was filtered off, and washed with 10% ethanol solution, water and isopropylether in turn, and 28.1 g of rough product was obtained. It was recrystallized in ethyl acetate, and 22.1 g of the captioned compound was obtained.

White edged crystals mp. 177.degree..about.179.degree. C.

Elemental analysis C.sub.17 H.sub.28 Cl.sub.3 N.sub.5

______________________________________ C H N______________________________________Calcd. 49.95 6.90 17.13Found 49.67 7.18 17.01______________________________________

EXAMPLE 2

Preparation of N.sup.1 -(4-chlorophenyl)-N.sup.5 -(3,4-dichlorobenzyl)-biguanide hydrochloride

To 20 g of N.sup.1 -cyano-N.sup.3 -(3,4-dichlorobenzyl)-guanidine and 13.5 g of 4-chloroaniline hydrochloride, 200 ml of water was added, which was heated and refluxed for 2 hours. When cooled gradually after reaction, crystals was precipiated. After filtering the crystals, by drying the crystals obtained by heat treatment in 200 ml of ethyl acetate, 21.8 g of the captioned compound was obtained.

White edged crystals mp. 238.degree..about.240.degree. C.

Elemental analysis C.sub.15 H.sub.15 Cl.sub.4 N.sub.5

______________________________________ C H N______________________________________Calcd. 44.25 3.71 17.20Found 44.19 3.66 17.17______________________________________

EXAMPLE 3

Preparation of N.sup.1 -(3,4-dichlorophenyl)-N.sup.5 -octyl-biguanide hydrochloride

In the same procedure as in Example 1 except that 3,4-dichlorobenzylamine hydrochloride was replaced by the equimolecular amount of 3,4-dichloroaniline hydrochloride, 28.2 g of the captioned compound was obtained.

White edged crystals mp. 176.degree..about.177.degree. C.

Elemental analysis C.sub.16 H.sub.26 Cl.sub.3 N.sub.5

______________________________________ C H N______________________________________Calcd. 48.68 6.64 17.74Found 48.52 6.50 17.77______________________________________

EXAMPLE 4

Preparation of N.sup.1 -benzyl-N.sup.5 -dodecyl-biguanide hydrochloride

To 20 g of N.sup.1 -cyano-N.sup.3 -benzyl-guanidine and 25.4 g of dodecylamine hydrochloride, 200 ml of mesitylene was added, which was heated and refluxed for 2 hours. After reaction, by the same operation as in Example 1, 23.7 g of the captioned compound was obtained.

White edged crystals mp. 135.degree..about.137.degree. C.

Elemental analysis C.sub.21 H.sub.38 ClN.sub.5

______________________________________ C H N______________________________________Calcd. 63.69 9.67 17.68Found 63.47 9.41 17.73______________________________________

EXAMPLE 5

Preparation of N.sup.1 -benzyl-N.sup.5 -decyl-biguanide hydrochloride

In the same manner as in Example 4 except that dodecylamine hydrochloride was replaced by the equimolecular amount of decylamine hydrochloride, 20.3 g of the captioned compound was obtained.

White edged crystals mp. 133.degree..about.135.degree. C.

Elemental analysis C.sub.19 H.sub.34 ClN.sub.5

______________________________________ C H N______________________________________Calcd. 62.02 9.31 19.03Found 62.29 9.57 18.91______________________________________

EXAMPLE 6

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(3-trifluoromethylphenyl)-biguanide hydrochloride

In the same manner as in Example 2 except that 4-chloroaniline hydrochloride was replaced by the equimolecular amount of 3-trifluoromethylaniline hydrochloride, 23.2 g of the captioned compound was obtained.

White edged crystals mp. 185.degree..about.188.degree. C.

Elemental analysis C.sub.16 H.sub.15 Cl.sub.3 F.sub.3 N.sub.5

______________________________________ C H N______________________________________Calcd. 43.61 3.43 15.89Found 43.57 3.24 16.10______________________________________

EXAMPLE 7

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(4-bromophenyl)-biguanide hydrochloride

In the same manner as in Example 2 except that 4-chloroaniline hydrochloride was replaced by the equimolecular amount of 4-bromoaniline hydrochloride, 21.6 g of the captioned compound was obtained.

White edged crystals mp. 223.degree..about.225.degree. C.

Elemental analysis C.sub.15 H.sub.15 BrCl.sub.3 N.sub.5

______________________________________ C H N______________________________________Calcd. 39.90 3.35 15.51Found 40.34 3.16 15.82______________________________________

EXAMPLE 8

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(4-iodophenyl)-biguanide hydrochloride

In the same manner as in Example 2 except that 4-chloroaniline hydrochloride was replaced by the equimolecular amount of 4-iodoaniline hydrochloride, 22.6 g of the captioned compound was obtained.

White edged crystals mp. 215.degree..about.217.degree. C.

Elemental analysis C.sub.15 H.sub.15 Cl.sub.3 IN.sub.5

______________________________________ C H N______________________________________Calcd. 36.14 3.03 14.05Found 36.25 2.80 14.21______________________________________

EXAMPLE 9

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(2,4-dichlorophenyl)-biguanide hydrochloride

In the same manner as in Example 2 except that 4-chloroaniline hydrochloride was replaced by the equimolecular amount of 2,4-dichloroaniline hydrochloride, 20.4 g of the captioned compound was obtained.

White edged crystals mp. 217.degree..about.220.degree. C.

Elemental analysis C.sub.15 H.sub.15 Cl.sub.5 N.sub.5

______________________________________ C H N______________________________________Calcd. 40.80 3.20 15.86Found 41.25 2.93 15.79______________________________________

EXAMPLE 10

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(3,4-dichlorophenyl)-biguanide hydrochloride

In the same manner as in Example 2 except that 4-chloroaniline hydrochloride was replaced by the equimolecular amount of 3,4-dichloroaniline hydrochloride, 21.7 g of the captioned compound was obtained.

White edged crystals mp. 205.degree..about.208.degree. C.

Elemental analysis C.sub.15 H.sub.14 Cl.sub.5 N.sub.5

______________________________________ C H N______________________________________Calcd. 40.80 3.20 15.86Found 41.03 2.94 15.94______________________________________

EXAMPLE 11

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(2,3,4-trichlorophenyl)-biguanide hydrochloride

In the same manner as in Example 2 except that 4-chloroaniline hydrochloride was replaced by the equimolecular amount of 2,3,4-trichloroaniline hydrochloride, 23.2 g of the captioned compound was obtained.

White edged crystals mp. 227.degree..about.228.degree. C.

Elemental analysis C.sub.15 H.sub.13 Cl.sub.6 N.sub.5

______________________________________ C H N______________________________________Calcd. 37.85 2.75 14.71Found 38.09 2.55 14.81______________________________________

EXAMPLE 12

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(3,4-dimethylphenyl)-biguanide hydrochloride

In the same manner as in Example 2 except that 4-chloroaniline hydrochloride was replaced by the equimolecular amount of 3,4-dimethylaniline hydrochloride, 20.3 g of the captioned compound was obtained.

White edged crystals mp. 190.degree..about.191.degree. C.

Elemental analysis C.sub.17 H.sub.20 Cl.sub.3 N.sub.5

______________________________________ C H N______________________________________Calcd. 50.95 5.03 17.48Found 50.66 4.78 17.56______________________________________

EXAMPLE 13

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(3,4-methylenedioxyphenyl)-biguanide hydrochloride

In the same manner as in Example 2 except that 4-chloroaniline hydrochloride was replaced by the equimolecular amount of 3,4-methylenedioxyaniline hydrochloride, 25.7 g of the captioned compound was obtained.

White edged crystals mp. 204.degree..about.206.degree. C.

Elemental analysis C.sub.16 H.sub.16 Cl.sub.3 N.sub.5 O.sub.2

______________________________________ C H N______________________________________Calcd. 46.12 3.87 16.81Found 45.83 3.69 16.97______________________________________

EXAMPLE 14

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(4-t-butylphenyl)-biguanide hydrochloride

In the same manner as in Example 2 except that 4-chloroaniline hydrochloride was replaced by the equimolecular amount of 4-t-butylaniline hydrochloride, 22.5 g of the captioned compound was obtained.

White edged crystals mp. 228.degree..about.230.degree. C.

Elemental analysis C.sub.19 H.sub.24 Cl.sub.3 N.sub.5

______________________________________ C H N______________________________________Calcd. 53.22 5.64 16.33Found 52.93 5.38 16.16______________________________________

EXAMPLE 15

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(4-ethylthiophenyl)-biguanide hydrochloride

In the same manner as in Example 2 except that 4-chloroaniline hydrochloride was replaced by the equimolecular amount of 4-ethylthioaniline hydrochloride, 20.7 g of the captioned compound was obtained.

White edged crystals mp. 199.degree..about.200.degree. C.

Elemental analysis C.sub.17 H.sub.20 Cl.sub.3 N.sub.5 S

______________________________________ C H N______________________________________Calcd. 47.18 4.66 16.18Found 47.12 4.46 16.04______________________________________

EXAMPLE 16

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(n-decyl)-biguanide hydrochloride

In the same manner as in Example 1 except that N.sup.1 -cyano-N.sup.3 -octyl-guanidine was replaced by the equimolecular amount of N.sup.1 -cyano-N.sup.3 -(n-decyl)-guanidine, 24.5 g of the captioned compound was obtained.

White edged crystals mp. 135.degree..about.137.degree. C.

Elemental analysis C.sub.19 H.sub.32 Cl.sub.3 N.sub.5

______________________________________ C H N______________________________________Calcd. 52.24 7.38 16.03Found 52.08 7.12 16.31______________________________________

EXAMPLE 17

Preparation of N.sup.1- -(3,4-dichlorobenzyl)-N.sup.5 -(1,1,3,3-tetramethylbutyl)-biguanide hydrochloride

In the same manner as in Example 1 except that N.sup.1 -cyano-N .sup.3 -oxtyl-guanidine was replaced by the equimolecular amount of N.sup.1 -cyano-N.sup.3 -(1,1,3,3-tetrabutyl)-guanidine, 23.1 g of the captioned compound was obtained.

White edged crystals mp. 196.degree..about.198.degree. C.

Elemental analysis C.sub.17 H.sub.28 Cl.sub.3 N.sub.5

______________________________________ C H N______________________________________Calcd. 49.95 6.90 17.13Found 49.78 6.81 17.01______________________________________

EXAMPLE 18

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -hexyl-biguanide hydrochloride

In the same manner as in Example 1 except that N.sup.1 -cyano-N.sup.3 -octyl-guanidine was replaced by the equimolecular amount of N.sup.1 -cyano-N.sup.3 -hexyl guanidine, 19.7 g of the captioned compound was obtained.

White edged crystals mp. 155.degree..about.157.degree. C.

Elemental analysis C.sub.15 H.sub.24 Cl.sub.3 N.sub.5

______________________________________ C H N______________________________________Calcd. 47.32 6.35 18.39Found 47.21 6.17 18.50______________________________________

EXAMPLE 19

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(2-ethoxyethyl)-biguanide hydrochloride

In the same manner as in Example 1 except that N.sup.1 -cyano-N.sup.3 -octyl-guanidine was replaced by the equimolecular amount of N.sup.1 -cyano-N.sup.3 -(2-ethoxyethyl)-guanidine, 23.1 g of the captioned compound was obtained.

White edged crystals mp. 170.degree..about.173.degree. C.

Elemental analysis C.sub.13 H.sub.20 Cl.sub.3 N.sub.5 O

______________________________________ C H N______________________________________Calcd. 42.35 5.47 18.99Found 42.09 5.18 18.73______________________________________

EXAMPLE 20

N.sup.1 -(3,4-dichlorobenzyl-N.sup.5 -[2-(2-hydroxyethoxy)ethyl]-biguanide hydrochloride

In the same manner as in Example 1 except that N.sup.1 -cyano-N.sup.3 -octyl-guanidine was replaced by the equimolecular amount of N.sup.1 -cyano-N.sup.3 -[2-(2-hydroxyethoxy)ethyl]-guanidine, 21.5 g of the captioned compound was obtained.

White edged crystals mp. 140.degree..about.142.degree. C.

Elemental analysis C.sub.13 H.sub.20 Cl.sub.3 N.sub.5 O.sub.2

______________________________________ C H N______________________________________Calcd. 40.59 5.24 18.20Found 40.30 5.01 18.03______________________________________

EXAMPLE 21

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(3-diethylaminopropyl)-biguanide

A rough product was obtained in the same manner as in Example 1 except that N.sup.1 -cyano-N.sup.3 -octyl-guanidine was replaced by the equimolecular amount of N.sup.1 -cyano-N.sup.3 -(3-diethylaminopropyl)-guanidine. The product was dissolved in 100 ml of methanol, and an equimolecular amount of 1N sodium hydroxide was added, which was concentrated at reduced pressure, and the residue was recrystallized in isopropanol, and 16.7 of the captioned compound was obtained.

White edged crystals mp. 156.degree..about.158.degree. C.

Elemental analysis C.sub.16 H.sub.26 Cl.sub.2 N.sub.6

______________________________________ C H N______________________________________Calcd. 51.48 7.02 22.51Found 51.21 6.83 22.76______________________________________

In the following Examples 22 to 26, using proper starting materials, the individual compounds were prepared in the same manner as in Example 1.

EXAMPLE 22

Preparation of N.sup.1 -(3,4-Dichlorobenzyl)-N.sup.5 -[3-(2-ethylhexyloxy)-propyl]-biguanide hydrochloride

White edged crystals mp. 200.degree..about.203.degree. C.

Elemental analysis C.sub.20 H.sub.34 Cl.sub.3 N.sub.5 O

______________________________________ C H N______________________________________Calcd. 51.45 7.34 15.00Found 51.12 7.62 14.82______________________________________

EXAMPLE 23

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -[(3-isopropoxy)propyl]-biguanide hydrochloride

White edged crystals mp. 158.degree..about.160.degree. C.

Elemental analysis C.sub.15 H.sub.24 Cl.sub.3 N.sub.5 O

______________________________________ C H N______________________________________Calcd. 45.41 6.10 17.65Found 45.17 6.02 17.53______________________________________

EXAMPLE 24

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -[(2-diethylamino)-ethyl]-biguanide

White edged crystals mp. 137.degree..about.140.degree. C.

Elemental analysis C.sub.15 H.sub.24 Cl.sub.2 N.sub.6

______________________________________ C H N______________________________________Calcd. 50.14 6.73 23.39Found 50.01 6.48 23.15______________________________________

EXAMPLE 25

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -[(3-butoxy)-propyl]-biguanide hydrochloride

White edged crystals mp. 181.degree..about.184.degree. C.

Elemental analysis C.sub.16 H.sub.26 Cl.sub.3 N.sub.5 O

______________________________________ C H N______________________________________Calcd. 46.78 6.38 17.05Found 46.49 6.27 17.21______________________________________

EXAMPLE 26

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -[3-(di-n-butylamino)propyl] -biguanide dihydrochloride

White edged crystals mp, 122.degree..about.124.degree. C.

Elemental analysis C.sub.20 H.sub.36 Cl.sub.4 N.sub.6

______________________________________ C H N______________________________________Calcd. 47.82 7.22 16.73Found 47.72 7.01 16.65______________________________________

EXAMPLE 27

Preparation of N.sup.1 -(4-chlorophenyl)-N.sup.5 -cyclohexylmethyl-biguanide hydrochloride

In the same manner as in Example 2 except that N.sup.1 -cyano-N.sup.3 -(3,4-dichlorobenzyl)-guanidine was replaced by the equimolecular amount of N.sup.1 -cyano-N.sup.3 -cyclohexylmethyl-guanidine, 20.4 g of the captioned compound was obtained.

White edged crystals mp. 240.degree..about.241.degree. C.

Elemental analysis C.sub.15 H.sub.23 Cl.sub.2 N.sub.5

______________________________________ C H N______________________________________Calcd. 52.33 6.73 20.34Found 52.24 6.61 20.48______________________________________

EXAMPLE 28

Preparation of N.sup.1 -(4-chlorophenyl)-N.sup.5 -(3-trifluoromethylphenyl)-biguanide hydrochloride

In the same manner as in Example 2 except that N.sup.1 -cyano-N.sup.3 -(3,4-dichlorobenzyl)-guanidine was replaced by the equimolecular amount of N.sup.1 -cyano-N.sup.3 -(3-trifluoromethylphenyl)-guanidine, 22.7 g of the captioned compound was obtained.

White edged crystals mp. 213.degree..about.215.degree. C.

Elemental analysis C.sub.15 H.sub.14 Cl.sub.2 F.sub.3 N.sub.5

______________________________________ C H N______________________________________Calcd. 45.93 3.60 17.86Found 46.03 3.55 18.04______________________________________

EXAMPLE 29

Preparation of N.sup.1 -(4-chlorophenyl)-N.sup.5 -(4-ethylthiophenyl)-biguanide hydrochloride

In the same manner as in Example 2 except that N.sup.1 -cyano-N.sup.3 -(3,4-dichlorobenzyl)-guanidine was replaced by the equimolecular amount of N.sup.1 -cyano-N.sup.3 -(4-ethylthiophenyl)-guanidine, 20.76 g of the captioned compound was obtained.

White edged crystals mp. 243.degree..about.245.degree. C.

Elemental analysis C.sub.16 H.sub.19 Cl.sub.2 N.sub.5 S

______________________________________ C H N______________________________________Calcd. 50.00 4.98 18.22Found 50.04 4.82 18.10______________________________________

EXAMPLE 30

Preparation of N.sup.1 -(4-chlorophenyl)-N.sup.5 -(4-chlorobenzyl)-biguanide hydrochloride

In the same manner as in Example 2 except that N.sup.1 -cyano-N.sup.3 -(3,4-dichlorobenzyl)-guanidine was replaced by the equimolecular amount of N.sup.1 -cyano-N.sup.3 -(4-chlorobenzyl)-guanidine, 21.6 g of the captioned compound was obtained.

White edged crystals mp. 225.degree..about.227.degree. C.

Elemental analysis C.sub.15 H.sub.16 Cl.sub.3 N.sub.5

______________________________________ C H N______________________________________Calcd. 48.34 4.33 18.79Found 48.32 4.19 18.86______________________________________

EXAMPLE 31

Preparation of N.sup.1 -(4-chlorophenyl)-N.sup.5 -(2,4-dichlorobenzyl)-biguanide hydrochloride

In the same manner as in Example 2 except that N.sup.1 -cyano-N.sup.3 -(3,4-dichlorobenzyl)-guanidine was replaced by the equimolecular amount of N.sup.1 -cyano-N.sup.3 -(2,4-dichlorobenzyl)-guanidine, 20.1 g of the captioned compound was obtained.

White edged crystals mp. 234.degree..about.236.degree. C.

Elemental analysis C.sub.15 H.sub.15 Cl.sub.4 N.sub.5

______________________________________ C H N______________________________________Calcd. 44.25 3.71 17.20Found 44.42 3.50 16.95______________________________________

EXAMPLE 32

Preparation of N.sup.1 -(4-chlorophenyl)-N.sup.5 -(4-acetylaminophenyl)-biguanide hydrochloride

In the same manner as in Example 2 except that N.sup.1 -cyano-N.sup.3 -(3,4-dichlorobenzyl)-guanidine was replaced by the equimolecular amount of N.sup.1 -cyano-N.sup.3 -(4-acetylaminophenyl)-guanidine, 18.6 g of the captioned compound was obtained.

White edged crystals mp. 253.degree..about.255.degree. C.

Elemental analysis C.sub.16 H.sub.18 Cl.sub.2 N.sub.6 O

______________________________________ C H N______________________________________Calcd. 50.40 4.76 22.04Found 50.63 4.51 21.92______________________________________

EXAMPLE 33

Preparation of N.sup.1 -(4-chlorophenyl)-N.sup.5 -(3,4-methylenedioxyphenyl)-biguanide hydrochloride

In the same manner as in Example 2 except that N.sup.1 -cyano-N.sup.3 -(3,4-dichlorobenzyl)-guanidine was replaced by the equimolecular amount of N.sup.1 -cyano-N.sup.3 -(3,4-methylenedioxyphenyl)-guanidine, 19.7 g of the captioned compound was obtained.

White edged crystals mp. 237.degree..about.239.degree. C.

Elemental analysis C.sub.15 H.sub.15 Cl.sub.2 N.sub.5 O.sub.2

______________________________________ C H N______________________________________Calcd. 48.93 4.11 8.69Found 48.76 3.95 8.47______________________________________

EXAMPLE 34

Preparation of N.sup.1 -(4-chlorophenyl)-N.sup.5 -(3,4-methylenedioxibenzyl)-biguanide hydrochloride

In the same manner as in Example 2 except that N.sup.1 -cyano-N.sup.3 -(3,4-di chlorobenzyl)-guanidine was replaced by the equimolecular amount of N.sup.1 -cyano-N.sup.3 -(3,4-methylenedioxybenzyl)-guanidine, 22.4 g of the captioned compound was obtained.

White edged crystals mp. 231.degree..about.233.degree. C.

Elemental analysis C.sub.16 H.sub.17 Cl.sub.2 N.sub.5 O.sub.2

______________________________________ C H N______________________________________Calcd. 50.27 4.48 18.32Found 50.44 4.29 18.31______________________________________

EXAMPLE 35

Preparation of N.sup.1 -(4-chlorobenzyl)-N.sup.5 -octyl-biguanide hydrochloride

In the same manner as in Example 1 except that 3,4-dichlorobenzylamine hydrochloride was replaced by the equimolecular amount of 2,4-chlorobenzylamine hydrochloride, 21.3 g of the captioned compound was obtained.

White edged crystals mp. 156.degree..about.158.degree. C.

Elemental analysis C.sub.17 H.sub.29 Cl.sub.2 N.sub.5

______________________________________ C H N______________________________________Calcd. 54.54 7.81 18.71Found 54.76 7.68 18.69______________________________________

EXAMPLE 36

Preparation of N.sup.1 -(4-chlorobenzyl)-N.sup.5 -(4-chlorobenzyl)-biguanide hydrochloride

Using 20.85 g of N.sup.1 -cyano-N.sup.5 -(4-chlorobenzyl)-guanidine and 17.8 g of 4-chlorobenzylamine hydrochloride, 21.4 g of the captioned compound was obtained by operating the same manner as in Example 1.

White edged crystals mp. 200.degree..about.202.degree. C.

Elemental analysis C.sub.16 H.sub.18 Cl.sub.3 N.sub.5

______________________________________ C H N______________________________________Calcd. 49.69 4.69 18.11Found 49.62 4.61 18.37______________________________________

EXAMPLE 37

Preparation of N.sup.1 -(4-chlorobenzyl)-N.sup.5 -decyl-biguanide hydrochloride

In the same manner as in Example 36 except that 4-chlorobenzylamine hydrochloride was replaced by the equimolecular amount of decylamine hydrochloride, 24.3 g of the captioned compound was obtained.

White edged crystals mp. 161.degree..about.163.degree. C.

Elemental analysis C.sub.19 H.sub.33 Cl.sub.2 N.sub.5

______________________________________ C H N______________________________________Calcd. 56.71 8.27 17.40Found 56.60 8.12 17.63______________________________________

EXAMPLE 38

Preparation of N.sup.1 -(4-chlorobenzyl)-N.sup.5 -dodecyl-biguanide hydrochloride

In the same manner as in Example 36 except that 4-chlorobenzylamine hydrochloride was replaced by the equimolecular amount of dodecylamine hydrochloride, 22.3 g of the captioned compound was obtained.

White edged crystals mp. 158.degree..about.160.degree. C.

Elemental analysis C.sub.21 H.sub.37 Cl.sub.2 N.sub.5

______________________________________ C H N______________________________________Calcd. 58.59 8.66 16.27Found 58.41 8.53 16.55______________________________________

EXAMPLE 39

Preparation of N.sup.1 -(4-chlorobenzyl)-N.sup.5 -isobutyl-biguanide hydrochloride

In the same manner as in Example 36 except that 4-chlorobenzylamine hydrochloride was replaced by the equimolecular amount of isobutylamine hydrochloride, 18.7 g of the captioned compound was obtained.

White edged crystals mp. 210.degree..about.221.degree. C.

Elemental analysis C.sub.13 H.sub.21 Cl.sub.2 N.sub.5

______________________________________ C H N______________________________________Calcd. 49.06 6.65 22.01Found 48.87 6.52 22.23______________________________________

EXAMPLE 40

Preparation of N.sup.1 -(4-chlorobenzyl)-N.sup.5 -(3,4-dichlorophenyl)-biguanide hydrochloride

In the same manner as in Example 36 except that 4-chlorobenzylamine hydrochloride was replaced by the equimolecular amount of 3,4-dichloraniline hydrochloride, 21.7 g of the captioned compound was obtained.

White edged crystals mp. 183.degree..about.185.degree. C.

Elemental analysis C.sub.15 H.sub.15 Cl.sub.4 N.sub.5

______________________________________ C H N______________________________________Calcd. 44.25 3.71 17.20Found 44.37 3.66 17.42______________________________________

EXAMPLE 41

Preparation of N.sup.1 -(3,4-dichlorophenyl)-N.sup.5 -(3,4-methylenedioxybenzyl)-biguanide hydrochloride

Using 21.5 g of N.sup.1 -cyano-N.sup.3 -(3,4-dichlorophenyl)-guanidine and 18.8 g of 3,4-methylenedioxybenzylamine hydrochloride, 27.3 g of the captioned compound was obtained by operating the same manner as in Example 1.

White edged crystals mp. 175.degree..about.177.degree. C.

Elemental analysis C.sub.16 H.sub.16 Cl.sub.3 N.sub.5 O.sub.2

______________________________________ C H N______________________________________Calcd. 46.12 3.87 16.81Found 46.01 3.76 16.97______________________________________

EXAMPLE 42

Preparation of N.sup.1 -(3,4-dichlorophenyl)-N.sup.5 -hexyl-biguanide hydrochloride

In the same manner as in Example 41 except that 3,4-methylenedioxybenzylamine hydrochloride was replaced by the equimolecular amount of hexylamine hydrochloride, 22.3 g of the captioned compound was obtained.

White edged crystals mp. 154.degree.-156.degree. C.

Elemental analysis C.sub.14 H.sub.22 Cl.sub.3 N.sub.5

______________________________________ C H N______________________________________Calcd. 45.85 6.05 19.10Found 45.66 6.17 19.03______________________________________

EXAMPLE 43

Preparation of N.sup.1 -(3,4-dichlorophenyl)-N.sup.5 -decyl-biguanide hydrochloride

In the same manner as in Example 41 except that 3,4-methylenedioxybenzylamine hydrochloride was replaced by the equimolecular amount of decylamine hydrochloride, 24.2 g of the captioned compound was obtained.

White edged crystals mp. 131.degree..about.133.degree. C.

Elemental analysis C.sub.18 H.sub.30 Cl.sub.3 N.sub.5

______________________________________ C H N______________________________________Calcd. 51.13 7.15 6.56Found 51.02 7.32 16.37______________________________________

EXAMPLE 44

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide lactate

Dissolving 20 g of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide hydrochloride in 200 ml of methanol, 24.5 ml of 5N NaOH was added, and the solvent was distilled away. The residual white solid was washed with water, suspended in 200 ml of water off, and 13 g of lactic acid was added, and dissolved by heating. Chilled in ice, precipitating crystals were filtered, and 12.14 g of the captioned compound was obtained.

White edged crystals mp. 45.degree..about.46.degree. C.

EXAMPLE 45

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide glycolate

The captioned compound was obtained in the same manner as in Example 44 except that lactic acid was replaced by glycolic acid.

White edged crystals mp. 109.degree..about.110.degree. C.

EXAMPLE 46

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide monomethanesulfonate

The captioned compound was obtained in the smae manner as in Example 44 except that lactic acid was replaced by methanesulfonic acid.

White edged crystals mp. 174.degree..about.176.degree. C.

EXAMPLE 47

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide hydrobromade

The captioned compound was obtained in the same manner as in Example 44 except that lactic acid was replaced by 47% hydrobromic acid.

White edged crystals mp. 120.degree..about.121.degree. C.

EXAMPLE 48

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide phosphate

The captioned compound was obtained in the same manner as in Example 44 except that lactic acid was replaced by phosphoric acid.

White edged crystals mp. 96.degree..about.98.degree. C.

EXAMPLE 49

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide dimethanesulfonate

Dissolving 25 g of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide hydrochloride in 250 ml of methanol, 30.6 ml of 5N NaOH was added, and the solvent was distilled away. The residual white solid was washed with water, and dissolved in 500 ml of acetone, and 14.7 g of methanesulfonic acid was added. The precipitating crystals were filtered off, and by recrystallizing from ethanol-ether, 25.8 g of the captioned compound was obtained.

White edged crystals mp. 171.degree..about.172.degree. C.

Elemental analysis C.sub.17 H.sub.27 N.sub.5 Cl.sub.2.2CH.sub.3 SO.sub.3 H

______________________________________ C H N______________________________________Calcd. 40.42 6.25 12.41Found 40.40 6.55 12.31______________________________________

REFERENCE EXAMPLE

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.3 -cyanoguanidine

Putting 63.7 g (300 mM) of 3,4-dichlorobenzylamine hydrochloride and 30.9 g (1.1 eq.) of sodium dicyanamide in 950 ml (15 V) of acetonitrile, the mixture was heated and refluxed for 3.5 hours. Distilling away the solvent, 600 ml of water was added to the residue (white solid) to disperse and wash. Obtained crystals were filtered off, and dispersed and washed in 600 ml of dichloromethane. Since residue of amine was recognized, the crystals were dispersed and washed again in 600 ml of water and 500 ml of dichloromethane. Crystals were filtered off, and dried overnight at 60.degree. C., and 57.7 g (78.9%) of the captioned compound was obtained.

White crystals mp. 173.degree. C.

Elemental analysis C.sub.9 H.sub.8 Cl.sub.2 N.sub.4

______________________________________ C H N______________________________________Calcd. 44.47 3.32 23.05Found 44.32 3.08 23.30______________________________________

EXAMPLE 50

Preparation of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide dihydrochloride

Suspending 10.0 g (41.1 mM) of N.sup.1 -(3,4-dichlorobenzyl)-N.sup.3 -cyanoguanidine and 5.31 g (1.0 eq.) of n-octylamine in 100 ml of xylene, 3.5 ml (1.1 eq.) of concentrated hydrochloric acid was added and stirred for several minutes. The reaction was continued for 8 hours in reflux by using Dean-Stark trap. Distilling away the solvent, and by ethanol coboiling, xylene was removed. The residue was dissolved in 40 ml of 70% acetonitrile, and 6.8 ml (2.0 eq.) of concentrated hydrochloric acid was added. Further adding 90 ml of 70% acetonitrile, the mixture was recrystallized. Obtained cystals were filtered off, dried overnight at 60.degree. C., and 14.3 g (78.2%) of white crystalline product was obtained. The obtained rough crystals were recrytallized twice with 70% acetonitrile (6 to 7 V), and 7.16 g (50.1%) of the captioned compound was obtained.

mp. 176.degree..about.178.degree. C.

Elemental analysis C.sub.17 H.sub.29 Cl.sub.4 N.sub.5

______________________________________ C H N______________________________________Calcd. 45.86 6.56 15.73Found 45.54 6.66 15.79______________________________________

Meanwhile, when the produced material (dihydrochloride) was recrystallized in a solvent of high water content (such as CH.sub.3 CN, ethanol), it was converted into the same monohydrochloride as obtained in Example 1.

______________________________________Prescription 1 of disinfectantMonobiguanide derivative of Example 1 5 gNonionic surface active agent 3.75 g(Polyoxyethylene phenylether)Distilled water for injection properWhole volume 100 mlPrescription 2 of disinfectantMonobiguanide derivative of Example 1 0.5 gNonionic surface active agent 0.375 g(Polyoxyethylene phenylether)Ethanol 83 mlDistilled water for injection properWhole volume 100 ml______________________________________ Antibacterial activity test

In order to test the antibacterial action of the compounds obtained in the examples in various organisms, the minimum inhibitory concentration (MIC) was determined according to the standard method of Japan Society of Chemotherapy. The culture medium for sensitivity disc was used (Chemotherapy, vol. 29, No. 1, 76-79, 1981). As the control drug, gluconic chlorohexydine (tradename "Hibiten" commercially available from Sumitomo Pharmaceutical) was used and tested similarly. The results are shown in Table 1.

The inoculum size was adjusted to 1.times.10.sup.6 cells/ml (0.07 to 0.16 at O.D. 600 nm).

TABLE 1__________________________________________________________________________ MIC .mu.g/ml (10.sup.6 cells/ml)__________________________________________________________________________ ExamplesStrain 1 2 3 4 5 6 7 8 9 10__________________________________________________________________________S. aureus FDA 209P 1.56 1.56 3.13 1.56 0.78 3.13 1.56 1.56 1.56 0.78S. aureus MRSA 57 1.56 3.13 3.13 1.56 1.56 3.13 1.56 1.56 1.56 0.39E. coli NIHJ JC-2 100 12.5 6.25 100 50 12.5 6.25 12.5 25 6.25K. pneumoniae NCTC 9632 -- -- -- -- -- -- -- -- -- --S. marcescens IFO 12648 100 25 100 100 50 25 25 25 25 6.25P. aeruginosa ATCC 10145 100 25 100 100 100 50 100 100 25A. calcoaceticus Ac-54 25 12.5 12.5 100 25 12.5 6.25 12.5 6.25 3.13__________________________________________________________________________ ExamplesStrain 11 12 13 14 15 16 17 18 19 20__________________________________________________________________________S. aureus FDA 209P 1.56 1.56 12.5 1.56 3.13 1.56 1.56 0.78 6.25 6.25S. aureus MRSA 57 0.78 1.56 12.5 1.56 3.13 1.56 1.56 0.78 6.25 6.25E. coli NIHJ JC-2 6.25 12.5 50 100 25 100 100 12.5 25 25K. pneumoniae NCTC 9632 -- -- -- -- -- -- -- -- -- --S. marcescens IFO 12648 12.5 25 50 50 100 100 100 12.5 50 50P. aeruginosa ATCC 10145 50 50 100 100 100 100 100 100 100 100A. calcoaceticus Ac-54 6.25 12.5 100 6.25 12.5 100 12.5 6.25 50 50__________________________________________________________________________ ExamplesStrain 21 22 23 24 25 26 27 28 29 30__________________________________________________________________________S. aureus FDA 209P 12.5 1.56 3.13 6.25 1.56 6.25 6.25 6.25 6.25 3.13S. aureus MRSA 57 12.5 1.56 3.13 6.25 1.56 6.25 6.25 6.25 6.25 0.78E. coli NIHJ JC-2 100 100 50 100 25 50 25 25 12.5 12.5K. pneumoniae NCTC 9632 -- -- -- -- -- 25 -- -- -- --S. marcescens IFO 12648 100 100 50 100 25 100 50 50 25 12.5P. aeruginosa ATCC 10145 100 100 100 100 100 100 100 100 100 25A. calcoaceticus Ac-54 100 100 50 100 25 100 12.5 12.5 12.5 6.25__________________________________________________________________________ ExamplesStrain 31 32 33 34 35 36 37 38 39 40__________________________________________________________________________S. aureus FDA 209P 1.56 25 50 25 3.13 3.13 1.56 6.25 12.5 1.56 S. aureus MRSA 57 0.78 12.5 50 25 3.13 3.13 1.56 6.25 12.5 1.56E. coli NIHJ JC-2 12.5 100 100 100 100 25 100 100 50 12.5K. pneumoniae NCTC 9632 -- -- -- -- -- 50 --S. marcescens IFO 12648 12.5 100 100 100 100 25 100 100 100 12.5P. aeruginosa ATCC 10145 25 100 100 100 100 100 100 100 100 100A. calcoaceticus Ac-54 6.25 100 100 100 25 25 100 100 50 6.25__________________________________________________________________________ Examples ControlStrain 41 42 43 44 45 46 49 agent__________________________________________________________________________S. aureus FDA 209P 12.5 3.13 6.25 1.56 1.56 1.56 1.56 1.56S. aureus MRSA 57 6.25 3.13 6.25 1.56 1.56 3.13 3.13 1.56E. coli NIHJ JC-2 25 12.5 100 100 100 100 100 1.56K. pneumoniae NCTC 9632 -- -- 50 -- -- -- -- --S. marcescens IFO 12648 25 6.25 100 100 100 100 100 25P. aeruginosa ATCC 10145 100 6.25 100 100 100 100 100 100A. calcoaceticus Ac-54 25 12.5 100 25 25 25 50 12.5__________________________________________________________________________ Bactericidal activity test

As to the compounds obtained in Examples 1 and 2, the bactericidal activity at room temperature (25.degree. C.) was measured by treating in a short time by reference to the phenol coefficient measurement method.

(1) Culture medium

For prior culture of test organism, Mueller Hinton Broth (DIFCD) was used. As growth culture medium of surviving cells in the test solution after bactericidal treatment, SCDLP culture medium inactivated by disinfectant was used ("Daigo" commercially available from Nippon Seiyaku).

(2) Test method

The compound obtained in each examples was dissolved in distilled water, and adjusted to a double concentration of the test concentration, and after aseptic filtering, the solution was diluted in steps of 1/2 in a range of Specified test concentration, and 50 .mu.l was dispensed in 96-well microplate. On the other hand, the test organism undergoing the prior culture for 16 hours twice was adjusted to 10.sup.8 cells/ml with sterilized distilled water in order to eliminate the effects of culture medium (0.3 at O.D. 660 nm), and diluted to 1/100 with 5 ml or 10 ml of sterilized distilled water to obtain 10.sup.6 cells/ml, and into 50 .mu.l of the test solution in the microplate, an equivalent 50 .mu.l was injected and mixed. After the lapse of 1 minute and 3 minutes, 5 .mu.l of the test solution was taken out from the microplate, and was suspended in 150 .mu.l of inactivated culture medium, and was cultivated for 16 to 18 hours at 37.degree. C. The survival of organisms in the test solution was judged by the turbidity, and the minimum bactericidal concentration in lapse of 1 minute and 3 minutes was determined. The result is shown in Table 2.

TABLE 2__________________________________________________________________________ Minimum Bactericidal Concentration .mu.g/ml (10.sup.6 cells/ml) Example 1 Example 2 Control agentStrain 1 min. 3 min. 1 min. 3 min. 1 min. 3 min.__________________________________________________________________________S. aureus FDA 209P 12.5 3.13 500 100 >1000 25S. aureus MRSA 57 6.25 .ltoreq.1.56 200 25 >1000 50E. coli NIHJ JC-2 3.13 .ltoreq.1.56 25 6.25 12.5 6.25K. pneumoniae NCTC 9632 3.13 3.13 12.5 12.5 3.13 .ltoreq.1.56S. marcescens IFO 12648 6.25 .ltoreq.1.56 25 6.25 12.5 6.25A. calcoaceticus Ac-54 3.13 3.13 12.5 6.25 6.25 6.25P. aeruginosa ATCC 10145 3.13 3.13 12.5 6.25 12.5 6.25P. cepacia 2315-C0010 200 50 200 50 >1000 >1000__________________________________________________________________________

Thus, the monobiguanide derivative (1) of the invention or its salt possesses a high bactericidal action and antibacterial action, and hence it may be preferably used as the disinfectant for the human or animal skin or the like.

Next, the examples of bis-biguanide derivative (2) of this invention are explained.

EXAMPLE 51

Preparation of 1,1'-[1,3-cyclohexanebis(methylene)]-bis[5-(4-chlorophenyl)biguanide]dihydrochloride

Refluxing 33 g (0.15 mols) of 1,3-di(aminomethyl) cyclohexane dihydrochloride and 26 g (0.3 mols) of dicyanamide sodium in buthanol for 12 hours at temperature of 140.degree. C., 51.3 g of 1,1'-[1,3-cyclohexanebis(methylene)]-bis(3-cyanoguanidine) was obtained.

Adding 5.52 g (0.02 mols) of this reaction product and 6.56 g (0.04 mols) of p-chloroaniline hydrochloride into .beta.-oxyethylether (tradename: Cellosolve), by refluxing for 3 hours at 140.degree. C. to react, 4.3 g of product was obtained. This product was recrystallized and refined in water and ethanol mixed solvent, and the captioned compound was obtained.

White edged crystals mp. 245.degree..about.248.degree. C. NMR (CDCl.sub.3 +DMSO-d6) .delta.: 1.1.about.1.9 (m, 8H), 2.8.about.3.5 (m, 6H), 6.95 (bs), 7.37 (dd, 4H, J=9 Hz 7.90 (bs)

EXAMPLE 52

Preparation of 1,1'-[1,3-cyclohexanebis(methylene)]-bis[5-(4-iodophenyl)biguanide]dihydrochloride

The captioned compound was obtained in the same manner as in Example 51 except that p-chloroaniline hydrochloride was replaced by the equimolecular amount of p-iodoaniline hydrochloride (Yield 5.1 g).

White edged crystals mp. 259.degree..about.261.degree. C.

EXAMPLE 53

Preparation of 1,1'-[1,3-cyclohexanebis(methylene)]-bis[5-(4-bromophenyl)biguanide]dihydrochloride

The captioned compound was obtained in the same manner as in Example 51 except that p-chloroaniline hydrochloride was replaced by the equimolecular amount of p-bromoaniline hydrochloride (Yield 4.7 g).

White edged crystals mp. 265.degree..about.267.degree. C.

EXAMPLE 54

Preparation of 1,1'-[1,3-cyclohexanebis(methylene)]-[5-(2,4-dichlorophenyl)biguanide]dihydrochloride

The captioned compound was obtained in the same manner as in Example 51 except that p-chloroaniline hydrochloride was replaced by the equimolecular amount of 2,4-dichloroaniline hydrochloride (Yield 5.7 g).

White edged crystals mp. 261.degree..about.265.degree. C.

EXAMPLE 55

Preparation of 1,1'-[1,3-cyclohexanebis(methylene)]-[5-(2,4-difluorophenyl)biguanide]dihydrochloride

The captioned compound was obtained in the same manner as in Example 51 except that p-chloroaniline hydrochloride was replaced by the equimolecular amount of 2,4-difluoroaniline hydrochloride (Yield 4.1 g).

White edged crystals mp. 212.degree..about.215.degree. C.

EXAMPLE 56

Preparation of 1,1'-[1,3-cyclohexanebis(methylene)]-[5-(4-t-butylphenyl)biguanide]dihydrochloride

The captioned compound was obtained in the same manner as in Example 51 except that p-chloroaniline hydrochloride was replaced by the equimolecular amount of p-t-butylaniline hydrochloride (Yield 3.6 g).

White edged crystals mp. 194.degree..about.197.degree. C.

EXAMPLE 57

Preparation of 1,1'-[1,3-cyclohexanebis(methylene)]-[5-(3,4-dimethylphenyl)biguanide]dihydrochloride

The captioned compound was obtained in the same manner as in Example 51 except that p-chloroaniline hydrochloride was replaced by the equimolecular amount of 3,4-dimethylaniline hydrochloride (Yield 3.8 g).

Pale yellow edged crystals mp. 162.degree..about.166.degree. C.

EXAMPLE 58

Preparation of 1,1'-[1,3-cyclohexanebis(methylene)]-[5-(4-ethylthiophenyl)biguanide]dihydrochloride

The captioned compound was obtained in the same manner as in Example 51 except that p-chloroaniline hydrochloride was replaced by the equimolecular amount of p-ethylthioaniline hydrochloride (Yield 5.4 g).

mp. 110.degree..about.113.degree. C.

EXAMPLE 59

Preparation of 1,1'-[1,3-cyclohexanebis(methylene)]-[5-(3-trifluoromethylphenyl)biguanide]dihydrochloride

The captioned compound was obtained in the same manner as in Example 51 except that p-chloroaniline hydrochloride was replaced by the equimolecular amount of p-trifluoromethylaniline hydrochloride (Yield 2.5 g).

White edged crystals mp. 240.degree..about.242.degree. C.

EXAMPLE 60

Preparation of 1,1'-[1,3-cyclohexanebis(methylene)]-[5-(2-ethylhexyl)biguanide]dihydrochloride

The captioned compound was obtained in the same manner as in Example 51 except that p-chloroaniline hydrochloride was replaced by the equimolecular amount of p-(2-ethylhexyl)aniline hydrochloride (Yield 4.6 g).

White edged crystals mp. 105.degree..about.107.degree. C.

EXAMPLE 61

Preparation of 1,1'-[1,3-cyclohexanebis(methylene)]-[5-(3,4-dichlorobenzyl)biguanide]dihydrochloride

The captioned compound was obtained in the same manner as in Example 51 except that p-chloroaniline hydrochloride was replaced by the equimolecular amount of 3,4-dichlorobenzylamine hydrochloride (Yield 5.3 g).

White edged crystals mp. 231.degree..about.233.degree. C.

EXAMPLE 62

Preparation of 1,1'-[1,3-cyclohexanebis(methylene)]-[5-(3,4-dichlorophenyl)biguanide]dihydrochloride

The captioned compound was obtained in the same manner as in Example 51 except that p-chloroaniline hydrochloride was replaced by the equimolecular amount of 3,4-dichloraniline hydrochloride (Yield 3.7 g).

White edged crystals mp. 259.degree..about.261.degree. C.

EXAMPLE 63

Preparation of 1,1'-[1,3-cyclohexanebis(methylene)]-[5-benzylbiguanide]dihydrochloride

The captioned compound was obtained in the same manner as in Example 51 except that p-chloroaniline hydrochloride was replaced by the equimolecular amount of benzylamine hydrochloride (yield 5.9 g).

White edged crystals mp. 208.degree..about.211.degree. C.

EXAMPLE 64

Preparation of 1,1-'[1,3-cyclohexanebis(methylene)]-[5-(4-chlorobenzyl)biguanide]dihydrochloride

The captioned compound was obtained in the same manner as in Example 51 except that p-chloroaniline hydrochloride was replaced by the equimolecular amount of 4-chlorobenzylamine hydrochloride (Yield 4.4 g).

White edged crystals mp. 204.degree..about.207.degree. C.

______________________________________Prescription 3 of disinfectantGluconic acid salt of Example 51 5 gNonionic surface active agent 3.75 g(Polyoxyethylene phenylether)Distilled water for injection properWhole volume 100 mlPrescription 4 of disinfectantGluconic acid salt of Example 51 0.5 gNonionic surface active agent 0.375 g(Polyoxyethylene phenylether)Ethanol 8.3 mlDistilled water for injection properWhole volume 100 ml______________________________________ Antibacterial activity test

To investigate the antibacterial actions of the compounds obtained in Examples 51 to 64, the minimum inhibitory concentration (MIC) was determined by employing the standard method of Japan Society of Chemotherapysame as mentioned above. The results are shown in Table 3.

TABLE 3__________________________________________________________________________ MIC .mu.g/ml (10.sup.6 cells/ml)__________________________________________________________________________ ExamplesStrain 51 52 53 54 55 56 57__________________________________________________________________________S. aureus FDA 209P 0.39 0.39 0.39 0.78 3.13 3.13 1.56S. epidermidis ATCC 12228 0.39 0.39 0.39 0.39 3.13 1.56 1.56E. coli NIHJ JC-2 3.13 6.25 3.13 6.25 25 25 12.5S. typhi NCTC 8393 1.56 6.25 3.13 6.25 6.25 12.5 6.25S. marcescens IFO 12648 6.25 12.5 6.25 50 50 50 50P. aeruginosa ATCC 10145 12.5 100 25 50 100 >100 100C. alficans IFO 1385 3.13 6.25 6.25 6.25 25 50 100A. fumigatus IFM 4942 125 250 125 250 400 >400 >400__________________________________________________________________________ Examples ControlStrain 59 61 62 63 64 agent__________________________________________________________________________S. aureus FDA 209P 1.56 3.13 0.78 3.13 1.56 1.56S. epidermidis ATCC 12228 1.56 1.56 0.39 1.56 1.56 0.78E. coli NIHJ JC-2 12.5 12.5 6.25 25 25 1.56S. typhi NCTC 8393 6.25 12.5 6.25 25 6.25 1.56S. marcescens IFO 12648 25 50 25 50 25 25P. aeruginosa ATCC 10145 >100 >100 100 >100 >100 >100C. alficans IFO 1385 100 100 6.25 100 100 5A. fumigatus IFM 4942 >200 >200 125 >200 >200 >200__________________________________________________________________________

Claims

1. A biguanide derivative or its salt selected from:

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide or its salt,

N.sup.1 -(3,4-dichlorophenyl)-N.sup.5 -octyl-biguanide or its salt,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(n-decyl)-biguanide or its salt,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(1,1,3,3-tetramethylbutyl)-biguanide or its salt,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -hexyl-biguanide or its salt,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(2-ethoxyethyl-(biguanide or its salt,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -[2-(2-hydroxyethoxy)ethyl]-biguanide or its salt,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -(3-diethylaminopropyl)-biguanide or its salt,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -[(2-diethylamino)-ethyl]-biguanide or its salt,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -[3-(di-n-butylamino)propyl]-biguanide or its salt,

N.sup.1 -(3,4-dichlorophenyl)-N.sup.5 -hexyl-biguanide or its salt, and

N.sup.1 -(3,4-dichlorophenyl)-N.sup.5 -decyl-biguanide or its salt.

2. A biguanide derivative or its salt selected from:

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide or its salt, and

N.sup.1 -(3,4-dichlorophenyl)-N.sup.5 -octyl-biguanide or its salt.

3. N.sup.1 -(3,4-Dichlorobenzyl)-N.sup.5 -octyl-biguanide or its salt.

4. The biguanide or its salt of claim 3, selected from:

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide hydrochloride,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide lactate,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide glucolate,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide monomethaesulfonate,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide hydrobromide,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide phosphate,

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide dimethanesulfonate, and

N.sup.1 -(3,4-dichlorobenzyl)-N.sup.5 -octyl-biguanide dihydrochloride.

5. A disinfectant composition comprising an effective amount of the biguanide derivative or its salt of claim 1, and its carrier.

6. A disinfectant composition comprising an effective amount of the biguanide derivative or its salt of claim 2, and its carrier.

7. A disinfectant composition comprising an effective amount of the biguanide or its salt of claim 3, and its carrier.